CN109517757B

CN109517757B - Manganese-oxidizing aeromonas hydrophila DS02 and application thereof

Info

Publication number: CN109517757B
Application number: CN201811428854.4A
Authority: CN
Inventors: 张越; 唐艳葵; 秦芷怡; 罗鹏宏; 康厚尧
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2018-11-27
Filing date: 2018-11-27
Publication date: 2020-08-07
Anticipated expiration: 2038-11-27
Also published as: CN109517757A

Abstract

The invention discloses a manganese-oxidized Aeromonas hydrophila DS02, which is deposited in the microbial culture collection (GDMCC) of Guangdong province with the preservation number of GDMCC NO.60416, and the manganese-oxidized Aeromonas hydrophila DS02 strain can normally grow in a Mn (II) concentration 10 mmol/L matrix, and Mn (II) is oxidized and can survive and synthesize biological manganese oxide under the condition of higher Mn (II).

Description

Manganese-oxidizing aeromonas hydrophila DS02 and application thereof

Technical Field

The invention relates to the technical field of biology, and particularly relates to a strain of manganese-oxidizing aeromonas hydrophila DS02 and application thereof.

Background

Manganese is a tenth element contained in the earth's crust, is second only to iron in metal elements, and is widely present in nature. Manganese oxide (MnO)₂) The catalyst has strong adsorption and catalytic oxidation capabilities, so that the catalyst is widely applied to the field of water purification and is mainly used for removing heavy metal ions and organic pollutants in water. Research in recent years has found that manganese oxides, which are widely present in the natural environment, are mainly formed by the action of microorganisms. The microorganism can catalyze and oxidize Mn (II) to generate oxide and can increase the oxidation rate of Mn (II) by several orders of magnitude (first order rate constant: biological oxidation 10-10)^-2Chemical oxidation of 10^-3-10^-5H). It has further been found that oxides of manganese formed by microbial conversion (i.e. biomasses) generally have higher adsorption properties than chemically synthesised oxides of manganese and that the primary products of biomasses have catalytic activity for the oxidation of Mn (ii). Due to the fact thatTherefore, the characteristics and applications of biological oxides of manganese are becoming a current research focus.

Typical p-Mn which has been isolated so far²⁺The bacteria with oxidizing ability are mainly classified into Pseudomonas (Pseudomonas), Bacillus (Bacillus), chrysosporium (L epothilone), chrysogenum (Metallogenin), geobacter (Pedomicrobium), Vibrio (Vibrio), Microdendrobium (Clothrix), Arthrobacter (Arthrobacter), Microbacterium hyphominis (Hyphomicrobium), and Spirospirium (Oceanospirillum), wherein few studies are reported about the manganese oxidizing property of Aeromonas hydrophila.

Azo dyes (organic compounds with aryl groups connected with two ends of azo groups) are the most widely applied synthetic dyes in textile and clothing printing and dyeing processes, the dyes can generate a plurality of carcinogenic aromatic amine substances in chemical reaction and decomposition, and aniline azo dyes are one of the most common products. At present, there are various methods for treating azo wastewater, and among them, the biological decolorization method is considered to be the most effective and environmentally friendly method.

The manganese oxide is a common mineral component in natural environments such as soil, sediment, lake, ocean and the like, has larger surface area and higher oxidation-reduction potential, and is one of reaction systems with higher activity in the environment. A large number of studies have shown that MnO₂Can catalyze single persulfate to decompose sulfate radicals, and has degradation effect on various pollutants entering the environment, such as phenols, aniline and the like. Aiming at the characteristic, the invention selects the widely used 2, 4-dimethylaniline in aniline azo dyes as an object to research the degradation characteristic and the reaction kinetics of the biological oxidation-reduction reaction of the aniline azo dyes under the oxidation action of biological manganese oxide.

The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Disclosure of Invention

Aiming at the technical problems, the invention provides a strain of manganese-oxidizing aeromonas hydrophila DS02 and application thereof, aiming at obtaining a manganese oxidation product generated under higher concentration and having brain-type and layer-type flaky manganese oxide morphological structures at different periods, and simultaneously the strain has higher manganese removal rate and manganese oxidation rate.

In order to achieve the purpose, the technical scheme provided by the invention is as follows:

a strain of manganese-oxidizing Aeromonas hydrophila DS02, wherein 16S rDNA of the Aeromonas hydrophila DS02 strain is shown as SEQ ID NO.1, the strain is stored in Guangdong province microbial culture collection (GDMCC) in 2018, 13 months, the storage address is Michelia intermedia 100, large institute 59, building 5, Guangdong province microbial research institute, and the storage number is GDMCC NO. 60416.

Wherein, the Aeromonas hydrophila DS02 synthesizes biological manganese oxide, and the operation method comprises the following steps: inoculating Aeromonas hydrophila DS02 into a liquid culture medium containing Mn (II) and having a constant pH value of 7.0-7.2, placing the liquid culture medium in a constant-temperature shaking table at 35 ℃ and a rotating speed of 200r/min for shake culture, after culturing for 15 days, centrifugally separating the liquid culture medium to obtain black precipitates, after vacuum freezing and drying the precipitates, grinding the precipitates into powder, and storing the powder at 4 ℃ to obtain the biological manganese oxide product.

Wherein the concentration of Mn (II) in the liquid culture medium containing Mn (II) is 1-50 mmol/L.

Wherein the concentration of Mn (II) in the liquid culture medium containing Mn (II) is 10-20 mmol/L, and the optimal concentration is 10 mmol/L.

Wherein Mn (II) is MnCl₂Or MnSO₄。

Wherein the liquid culture medium contains 2.0g of peptone, 0.5g of yeast powder and K per 1L of liquid culture medium₂HPO₄0.1g、MgSO₄·7H₂O 0.2g、NaNO₃0.2g、CaCl₂0.1g、(NH₄)₂CO₃0.1g, 2.38g of hydroxyethylpiperazine ethanethiosulfonic acid (HEPES), pH: 7.0-7.2.

The application of manganese-oxidized aeromonas hydrophila DS02 in degrading azo dyes comprises the following steps: adding the obtained biological manganese oxide synthesized by Aeromonas hydrophila DS02 into reaction solution containing 2, 4-dimethylaniline, oscillating in a shaker at the rotation speed of 200r/min for 30 min, stopping oscillation, and adding single persulfate (KHSO)₅·0.5KHSO₄·0.5K₂SO₄) And degrading at 25 ℃ for 80-120 minutes.

The reaction solution containing 2, 4-dimethylaniline is a solution formed by dissolving 2, 4-dimethylaniline in methanol, the concentration of the 2, 4-dimethylaniline in the reaction solution containing 2, 4-dimethylaniline is 50 mg/L, and the pH value of the reaction solution containing 2, 4-dimethylaniline is 6.75, wherein the volume concentration of the methanol is 20%.

Wherein the adding amount of the biological manganese oxide is 0.4 g/L, namely 0.4g of biological manganese oxide is added into the reaction solution containing 2, 4-dimethylaniline of 1L.

Wherein the single persulfate is added in an amount of 0.4 g/L, that is, 0.4g of the single persulfate is added into the reaction solution containing 2, 4-dimethylaniline in 1L.

The Aeromonas Hydrophila DS02 is obtained by screening, separating and purifying stream sediments near manganese ore in Daxin county, Chongxi city, Guangxi province, and the Aeromonas Hydrophila DS02 is gram-negative bacillus. In the solid culture medium, the small white round colonies with smooth surfaces are formed. The scanning electron microscope shows the short rod ellipsoid shaped bacteria, the length is 1.2-1.4 μm, the width is 0.4-0.6 μm.

Compared with the prior art, the invention has the following beneficial effects:

the manganese-oxidized Aeromonas hydrophila DS02 strain can normally grow in a matrix with the concentration of Mn (II) being 10 mmol/L, Mn (II) is oxidized, and the manganese-oxidized Aeromonas hydrophila DS02 strain can survive and synthesize biological manganese oxide under the condition of high-concentration Mn (II), so that Mn (II) in a water body is removed, and the application prospect of the biological manganese oxide is wide.

Drawings

FIG. 1 is a phylogenetic tree diagram of Aeromonas hydrophila DS 02.

FIG. 2 is a scanning electron microscope image of Aeromonas hydrophila DS02 synthesized biological manganese oxide in different liquid culture media; wherein, FIG. 2A and FIG. 2B are scanning electron micrographs of Aeromonas hydrophila DS02 in the stationary phase of growth in a liquid culture medium without Mn (II) added; FIGS. 2C and 2D are scanning electron micrographs of Aeromonas hydrophila DS02 synthesized biological manganese oxide in Mn (II) -containing liquid medium.

FIG. 3 is an OD600 of Aeromonas hydrophila DS02 at various concentrations of Mn (II) when cultured for 24 h.

FIG. 4 is a graph of Aeromonas hydrophila DS02 for Mn (II) removal and biomanganese oxide synthesis at various concentrations of Mn (II).

FIG. 5 is a graph showing the growth of Aeromonas hydrophila DS02 at a concentration of 10 mmol/L Mn (II).

FIG. 6 is an XRD pattern of the biological manganese oxide product produced by Aeromonas hydrophila DS 02.

FIG. 7 is a graph showing the change of the concentration of 2, 4-dimethylaniline in the reaction of catalyzing single persulfate to degrade 2, 4-dimethylaniline by a biological manganese oxide product generated by Aeromonas hydrophila DS 02.

Detailed Description

The following detailed description is to be read in connection with the accompanying drawings, but it is to be understood that the scope of the invention is not limited to the specific embodiments. Mn (II) used in the examples is commercially available AR-grade MnCl₂Or MnSO₄The unit r/min used represents 1 revolution per minute.

The liquid medium, solid medium and liquid medium containing Mn (II) used in the following examples were all the media shown below:

the liquid medium (g/L) comprises peptone 2.0g, yeast powder 0.5g, and K₂HP O₄0.1g，MgSO₄·7H₂O0.2g，NaNO₃0.2g，CaCl₂0.1g，(NH₄)₂CO₃0.1g, HEPES 2.38g, pH: 7.0-7.2. Adding MnCl into the liquid culture medium₂Or MnSO₄Mn (II) is formed and Mn (II) is brought to final concentrations of 1.0 mmol/L, 10.0 mmol/L, 20.0 mmol/L0, 30.0 mmol/L1, 40.0 mmol/L and 50.0 mmol/L respectively, that is to say, liquid media (for preventing MnCl) containing Mn (II) at concentrations of 1.0 mmol/L, 10.0 mmol/L, 20.0 mmol/L, 30.0 mmol/L, 40.0 mmol/L and 50.0 mmol/L are obtained respectively₂Or MnSO₄High temperature sterilization for oxidation, and filtering and sterilizing with 0.22 μm microporous membrane).

Solid Medium (g/L) to the liquid medium obtained above, 2.0% agar was added, and MnCl was further added₂Or MnSO₄Mn (II) was formed and Mn (II) was sterilized at a final concentration of 2 mmol/L at 121 ℃ for 20 minutes, and the sterilized medium was poured into a sterilized petri dish in a clean bench, thereby preparing a flat solid medium and a slant solid medium for seed preservation (for prevention of MnCl)₂Or MnSO₄High temperature sterilization for oxidation, and filtering and sterilizing with 0.22 μm microporous membrane).

Example 1

Screening, isolation and characterization of Aeromonas hydrophila DS02

1. Screening, domestication and separation of Aeromonas hydrophila DS02

Aeromonas hydrophila hydrophylla DS02 is screened from a stream sediment sample near manganese ore in New county, Chong, left and city, Guangxi province, and is screened, separated and purified to obtain a gram-negative bacterium with the performance of synthesizing biological manganese oxide, and the specific steps are as follows:

taking a sample of stream sediment near manganese ore in Chonguo city, Chongxi province of Guangxi province, adding 1.0g of soil into 9.0m L sterile water, and oscillating for 30 minutes (150)r/min, 25 deg.C, standing for 10 minutes, sucking 1.0m L supernatant, adding into 100m L liquid culture medium containing 1.0 mmol/L Mn (II), culturing for 10 days (150r/min, 25 deg.C), shaking, culturing for 10 days, sucking 1.0m L culture medium, adding into screening culture medium containing 10 mmol/L Mn (II), culturing and acclimating for 10 days, diluting the culture medium with sterile water to 10 of the original solution^-1And 10^-2And (3) sucking the original solution and the diluent, respectively 0.1m L, coating the original solution and the diluent on a solid culture medium plate with the diameter of 90mm, wherein the plate contains 10 mmol/L Mn (II), selecting colonies showing brown or black after culturing for 7-20 days, detecting the colonies by using a L BB method, and determining strains capable of changing colorless L BB (L leucoBerkelin Blue) into Blue as positive strains and separately plating and storing the positive strains.

The DS02 obtained by the above screening, acclimatization and separation was gram-negative bacteria. The medium is white round microcolony with smooth surface in solid culture medium, and scanning electron microscope shows short rod ellipsoid shaped bacteria with length of 1.2-1.4 μm and width of 0.4-0.6 μm, and the colony morphology of DS02 is shown in Table 1. Scanning electron microscope pictures of the medium without Mn (II) added to reach the growth stationary phase are shown in FIGS. 2A and B, which show that the strain has the morphological characteristics of ellipsoid and is provided with grooves; scanning electron micrographs of the biomassic oxide synthesized in the Mn (II) -containing medium are shown in FIGS. 2C and D, which show that the biomassic oxide is tightly wrapped around the thalli and forms a 'brain-type' and layer-type sheet-like structure. FIG. 6 is an XRD pattern of a manganese oxide product showing the composition of MnCO₃And Mn₂O₃。

TABLE 1

According to the methods of GBT18652-2002 'pathogenic aeromonas hydrophila test method', 'Manual of general bacteria System identification' and 'microbiology experiment' (second edition), experiments such as morphological observation, gram staining, oxidase, catalase, glucose hydrolysis, denitrification and the like are carried out on the strain DS 02. The DS02 is determined to belong to gram-negative bacteria, the DS02 is determined to belong to facultative aerobic bacteria as a result of the motility test, the acidic or non-acidic products can be generated by utilizing glucose, indole and amylase are generated, the specific results are shown in Table 2, the results of the DS02 gram staining, oxidase test and denitrification test are negative, and the results of the catalase test and the glucose hydrolase test are positive. The physiological and biochemical results are consistent with those of aeromonas hydrophila, and further prove the 16S rDNA sequencing and the homology comparison conclusion of the evolutionary tree.

TABLE 2

Physiological index	Results
		Strain Length (μm)	1.4-1.5
Gram stain	-
		Motility test	+
Indole test	+
		Starch hydrolysis test	+
Hydrolysis test of glucose	-
		Oxidase test	+
Catalase assay	-

+ positive; negative.

2. Construction of 16S rDNA phylogenetic tree constructed based on Aeromonas hydrophila DS02

The specific steps of 16S rDNA identification are as follows:

the reported 16S rDNA universal primer 27F (5'-AGA GTT TGA TCC TGG CTC AG-3') is shown in SEQ ID NO.2, and 492R (5'-AAG GAG GTG ATC CAG CCG CA-3') is shown in SEQ ID NO.3, and the amplification of the target gene is carried out. The PCR reaction conditions are as follows: 2 minutes at 98 ℃; 10s at 98 ℃; 30s at 57 ℃; 40s at 72 ℃; 32 cycles; 10 minutes at 72 ℃. The PCR products were recovered and sequenced (by Meiji Biotech, Shanghai).

The 16S rDNA sequence of DS02 was introduced into GenBank for homology alignment, as shown in fig. 1. The result shows that the most similar strain is Aeromonas hydrophila, and the similarity is 99%. The strain can be judged to belong to Aeromonas hydrophila by combining morphological characteristics and physiological and biochemical characteristics, and is named as Aeromonas hydrophila DS 02.

Example 2

Characteristic study of Aeromonas hydrophila DS02 synthesized biological manganese oxide

1. Growth and synthesis of biological manganese oxide by Aeromonas hydrophila DS02 at various Mn (II) concentrations;

inoculating 10% of Aeromonas hydrophila DS02 seed solution into liquid culture medium containing different Mn (II) concentrations, wherein Mn (II) concentrations are 1.0 mmol/L, 10.0 mmol/L, 20.0 mmol/L, 30.0 mmol/L, 40.0 mmol/L and 50.0 mmol/L respectively, and the culture medium pH is 7.0-7.2. after inoculation, shaking and culturing for 1 day (150r/min, 35 ℃), measuring the bacteria concentration (OD600), and after 6 days, measuring the manganese removal rate and manganese oxidation rate, centrifuging supernatant manganese content with atomic absorption assay bacteria solution, measuring the biological manganese oxide concentration with L BB method, as shown in FIG. 3 (OD600 of DS02 at different Mn (II) concentrations), the Aeromonas hydrophila DS02 shows that the manganese removal rate is slightly higher than that of Mn (Mn) at Mn (II) concentration, and the Mn (Mn) concentration of Mn (Mn) is slightly higher than that of Mn (Mn) at Mn (II) concentration, and the Mn (Mn) concentration of H5) is slightly higher than that of Mn (Mn) of H, and Mn (Mn) is slightly higher than that of Mn (Mn) of H, and Mn (II) is shown in FIG. 3, and (III) shows a similar to be increased (L).

2. Growth curves of Aeromonas hydrophila DS02 at optimal Mn (II) concentrations.

The Aeromonas hydrophila DS02 seed solution 1/10 was inoculated into a liquid culture medium containing 10 Mmol/L Mn (II) for 6 days, wherein the bacterial concentration (OD600) was periodically measured, as shown in FIG. 5 (growth curve of Aeromonas hydrophila DS02 at 10 Mmol/L Mn (II)) the results show that the growth of Aeromonas hydrophila DS02 is only slightly affected by the production of biological manganese oxide.

In conclusion, Aeromonas hydrophila DS02 has Mn (II) oxidation activity at pH7.0-7.2 and 35 ℃, can grow and synthesize biological manganese oxide at the Mn (II) concentration of not more than 50 mmol/L, and obtains the optimal biological manganese oxide generation rate at the Mn (II) concentration of 10 mmol/L.

Example 3

An application of manganese oxidizing strain Aeromonas hydrophila DS02 in degrading 2, 4-dimethylaniline comprises the following steps:

1. synthesis method of Aeromonas hydrophila DS02 biological manganese oxide

Inoculating 10% of Aeromonas hydrophila DS02 seed liquid into a liquid culture medium containing Mn (II) with the concentration of 10.0 mmol/L, enabling the pH of the culture medium to be 7.0-7.2, after inoculation, carrying out shake culture in a constant-temperature shaking table at the temperature of 35 ℃ and the rotating speed of 200r/min for 15 days, collecting biological manganese oxide, carrying out vacuum freezing and drying for 24h, grinding the biological manganese oxide into powder, and storing the powder in a refrigerator at the temperature of 4 ℃ for later use.

2. An application of manganese oxidizing strain Aeromonas hydrophila DS02 in degrading 2, 4-dimethylaniline is as follows:

adding the spare biological manganese oxide into 50m L reaction liquid containing 2, 4-dimethylaniline, wherein the adding amount of the biological manganese oxide is 0.4 g/L, placing the mixture in a shaking table at the rotating speed of 200r/min, oscillating for 30 minutes, then uniformly distributing the biological manganese oxide, stopping oscillating, adding single persulfate solid, the adding amount of the single persulfate is 0.4 g/L, oscillating at the temperature of 25 ℃ for degradation, wherein the pH value of the reaction liquid containing the 2, 4-dimethylaniline is 6.75, the concentration of the 2, 4-dimethylaniline in the reaction liquid containing the 2, 4-dimethylaniline is 50 mg/L, and the reaction liquid containing the 2, 4-dimethylaniline is obtained by dissolving the 2, 4-dimethylaniline by adopting methanol with the volume concentration of 20%.

The sampling time was 5, 10, 15, 30, 45, 60, 80, 100, 120 minutes after the start of the reaction degradation, the sampling volume was 2M L, and 0.1M L0.1.0.1M Na was added simultaneously₂SO₃After the obtained sample passes through an organic filter membrane of 0.22 mu m, the obtained sample is placed in a sample injector of a high performance liquid chromatography (HP L C) instrument (the parameters are shown in a table 3) to measure the concentration of 2, 4-dimethylaniline, and the result is obtained after measurement, the Aeromonas hydrophila hydrophylla DS02 can catalyze a single persulfate to degrade a certain amount of 2, 4-dimethylaniline (shown in a figure 7). As shown in a figure 7, the degradation rate of the 2,4-DMA can reach more than 99 percent when the reaction is carried out for 80 minutes, and the substrate is completely degraded when the reaction is carried out for 120 minutes.

TABLE 3 high performance liquid chromatography parameter settings

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

SEQUENCE LISTING

<110> Guangxi university

<120> manganese-oxidizing aeromonas hydrophila DS02 and application thereof

<130> intellectual Property agency of West holy, Beijing

<160>3

<170>PatentIn version 3.3

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<211>1292

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<213>Aeromonas hydrophila

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cgggcggtgt gtacaaggcc cgggaacgta ttcaccgcaa cattctgatt tgcgattact 60

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tagccctggc cgtaagggcc atgatgactt gacgtcatcc ccaccttcct ccggtttatc 240

accggcagtc tcccttgagt tcccaccatt acgtgctggc aacaaaggac aggggttgcg 300

ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacagcc atgcagcacc 360

tgtgttctga ttcccgaagg cactcccgta tctctacagg attccagaca tgtcaaggcc 420

aggtaaggtt cttcgcgttg catcgaatta aaccacatgc tccaccgctt gtgcgggccc 480

ccgtcaattc atttgagttt taaccttgcg gccgtactcc ccaggcggtc gatttaacgc 540

gttagctccg gaagccacgt ctcaaggaca cagcctccaa atcgacatcg tttacggcgt 600

ggactaccag ggtatctaat cctgtttgct ccccacgctt tcgcacctga gcgtcagtct 660

ttgtccaggg ggccgccttc gccaccggta ttcctccaga tctctacgca tttcaccgct 720

acacctggaa ttctaccccc ctctacaaga ctctagctgg acagttttaa atgcaattcc 780

caggttgagc ccggggcttt cacatctaac ttatccaacc gcctgcgtgc gctttacgcc 840

cagtaattcc gattaacgct tgcaccctcc gtattaccgc ggctgctggc acggagttag 900

ccggtgcttc ttctgcgagt aacgtcacag ttgatacgta ttaggcatca acctttcctc 960

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ccattacctc accaactagc taatcccacc tgggcatatc caatcgcgca aggcccgaag 1200

gtcccctgct ttcccccgta gggcgtatgc ggtattagca gtcgtttcca actgttatcc 1260

ccctcgactg ggcaatttcc caggcattac tc 1292

<210>2

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Claims

1. Manganese-oxidizing aeromonas hydrophila (Aeromonas hydrophila) DS02, characterized in that the Aeromonas hydrophila (A)Aeromonas hydrophila) The 16S rDNA of the DS02 strain is shown in SEQ ID NO.1, and the strain is preserved in Guangdong province microbial culture collection center (GDMCC) in 2018, 7 and 13 months, and the preservation address is Guangzhou city Michelia Tokyo 100 # louse 59-floor 5-floor Guangdong province microbial research institute, and the preservation number is GDMCC number 60416.

2. The manganese-oxidizing aeromonas hydrophila of claim 1 (f)Aeromonas hydrophila) The application of DS02 in the synthesis of biological manganese oxide is characterized in that the operation method comprises the following steps: aeromonas hydrophila (f) (Aeromonas hydrophila) Inoculating DS02 into liquid culture medium containing Mn (II) and having pH value of 7.0-7.2, culturing at 35 deg.C under shaking at 200r/min for 15 days, separating the liquid culture medium to obtain black precipitate, and oven drying the precipitate to obtain biological manganese oxide product.

3. The use according to claim 2, wherein the concentration of Mn (II) in the liquid culture medium containing Mn (II) is 1-50 mmol/L.

4. The use according to claim 2 or 3, wherein the concentration of Mn (II) in the liquid culture medium containing Mn (II) is 10-20 mmol/L.

5. Use according to claim 2, characterized in that: mn (II) is MnCl₂Or MnSO₄。

6. Use according to claim 2, characterized in that: what is needed isThe liquid culture medium contains peptone 2.0g, yeast powder 0.5g, and K per 1L liquid culture medium₂HPO₄0.1 g、MgSO₄·7H₂O 0.2 g、NaNO₃0.2 g、CaCl₂0.1g、(NH₄)₂CO₃0.1g, 2.38g of hydroxyethylpiperazine ethanethiosulfonic acid, pH: 7.0-7.2.

7. Use of the manganese oxidizing aeromonas hydrophila DS02 of claim 1 for degrading azo dyes, wherein: adding the biological manganese oxide synthesized in the application of claim 2 into a reaction solution containing 2, 4-dimethylaniline, oscillating at the rotating speed of 200r/min for 30 minutes, stopping oscillating, adding single persulfate, and degrading at 25 ℃ for 80-120 minutes.

8. The use according to claim 7, wherein the reaction solution containing 2, 4-dimethylaniline is a solution obtained by dissolving 2, 4-dimethylaniline in methanol, the concentration of 2, 4-dimethylaniline in the reaction solution containing 2, 4-dimethylaniline is 50 mg/L, and the pH of the reaction solution containing 2, 4-dimethylaniline is 6.75.

9. The use according to claim 7, wherein the amount of the biological manganese oxide added is 0.4 g/L.

10. The use according to claim 7, wherein the amount of the persulfate salt added is 0.4 g/L.