CN109511674A - Application of the NaHS as plant stress-resistance agent - Google Patents
Application of the NaHS as plant stress-resistance agent Download PDFInfo
- Publication number
- CN109511674A CN109511674A CN201811562357.3A CN201811562357A CN109511674A CN 109511674 A CN109511674 A CN 109511674A CN 201811562357 A CN201811562357 A CN 201811562357A CN 109511674 A CN109511674 A CN 109511674A
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- Prior art keywords
- nahs
- plant
- stress
- resistance agent
- aqueous solution
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
Application the invention discloses NaHS as plant stress-resistance agent.Matching while using when use, it is 50-100 μm of olL that NaHS, which is first configured to concentration,‑1Aqueous solution, be sprayed on the mature leaf of plant.Above-mentioned NaHS aqueous solution is NaHS by slowly releasing H in water2S/HS‑, exercise its function of inhibiting guard cell's development, promote the synthesis of plant surface wax.Effect of the invention is obvious, and good water solubility is at low cost, safe and non-toxic, and preparation is simple.Plant stress-resistance agent of the present invention can also supplement sulphur required for plant (S) and sodium (Na) simultaneously, have the function of foliar fertilizer.
Description
Technical field
A kind of a kind of application the present invention relates to NaHS as plant stress-resistance agent, and in particular to NaHS (NaHS)
The new application and its application method of aqueous solution enhancing stress resistance of plant.
Background technique
Environment-stress (arid, waterlogging, salt damage, low temperature, pest and disease damage etc.) be influence and limitation production estimation it is main because
One of element.In crop production, by creating good agroecological environment, cultivating to have and resist the excellent of poor environment character
Kind etc. is always that scientists make great efforts the research field explored to improve the yield and quality of crop.
Blade is that aerial nutrition organs is uniquely completely exposed in plant, plays what plant mutually exchanged with environment
Key player is substantially carried out transpiration and photosynthesis.The transpiration of plant includes transpiration and Cuticular Transpiration, maturation
The 95% of leaf transpiration amount is carried out by stomata, but under conditions of serious water shortage, and blade epidermis stomata is closed, under stomatal conductance
Drop, Cuticular Transpiration become the important channel of plant dehydration.The raw organ surface of the gas of terrestrial plant is covered with one layer and is formed by wax
Adipose membrane, be the first barrier of plant resistant external environment stimulation, it is anti-some researches show that, Plant cuticle wax and plant
There is extremely close relationship between drought, i.e., the content of wax, thickness and ultra microstructure will affect the penetrating of moisture and
Transport, makes Cuticular Transpiration be affected.Therefore, stomata development, distribution density and the wax content on leaf epidermis are plant replies
The importance of environment drought stress.Furthermore current research also found, epicutile wax in addition to participate in control moisture loss process other than,
Can also reflect in visible light UV radiation, slow down temperature stress sensibility, prevent thallus infringement etc..
NaHS (NaHS) is typically used as industrial chemicals, generates hydrogen sulfide (H after being dissolved in water2S) gas, therefore become
Most common H2S donor.H2S is a kind of completely new gaseous signal molecule of discovery after plant relaying NO and CO, is had in recent years big
Amount report proves H2S can be participated in adjusting the intracorporal a variety of physiological metabolism processes of plant and be improved stress resistance of plant, wherein H2S can
Enhance the drought resistance of plant to close by induction stomata.However plant is during being subjected to drought stress, except through
Stomatal movement is resisted outside drought stress, there are also Nonsto-matal factor while in action, including the cutin and edge as blade framework
Embedded in wax therein.So far, H2S is during enhancing plant drought resistance, other than induction stomata is closed, if to guarantor
The development of guard cell and surface wax are synthetically produced influence, have not been reported.
Summary of the invention
The present invention is intended to provide a kind of application of NaHS as plant stress-resistance agent, NaHS is by promoting plant surface wax
Matter synthesis inhibits guard cell's development to enhance the new application of stress resistance of plant.
The present invention is realized by following technical solution: NaHS being configured to certain density aqueous solution, is sprayed
Plant leaf blade surface has the function of enhancing plant drought resistance.It is in particular in that reduction guard cell's volume, reduction stomata are close
Degree, promote plant surface wax synthesis etc., thus prediction reach in agricultural production improve efficiency of water application, reflection it is visible
In light UV radiation, slow down temperature stress sensibility, prevent thallus infringement etc. enhancing stress resistance of plant purpose.
The present invention is according to the natural growth of plant seedlings and physiological metabolism feature, by screening the condition with system extensively
What test was completed.Find that NaHS is configured to certain density aqueous solution is sprayed on blade face, there is inhibition guard cell under study for action
It develops, promote the synthesis of plant surface wax, reduction plant moisture is lost to wait the work for resisting biology and abiotic stress to reach
With.
Application of the NaHS provided by the invention as plant stress-resistance agent, it is 5-500 μm of olL that NaHS, which is configured to concentration,-1
Aqueous solution.
Preferably, in above-mentioned application, it is 50-100 μm of olL that NaHS, which is configured to concentration,-1Aqueous solution.
Application method the present invention provides NaHS as plant stress-resistance agent: it using NaHS as when plant stress-resistance agent, uses
When matching while using, NaHS is first configured to aqueous solution, is then sprayed on the mature leaf upper surface of plant.
The present invention is using tomato, cucumber, tobacco and arabidopsis as experimental material, research shows that above-mentioned NaHS aqueous solution can be shown
It writes and reduces guard cell's volume, reduce stomatal frequency, promote leaf surface waxes synthesis, so as to increase the water conservation energy of plant
Power and the ability for resisting other environment-stress.
Studies have shown that above-mentioned NaHS aqueous solution is NaHS by slowly releasing H in water2S/HS-, exercise it and inhibit to protect
Guard cell development, the function of promoting the synthesis of plant surface wax.
Beneficial effects of the present invention:
Effect of the invention is obvious, and good water solubility is at low cost, safe and non-toxic, and preparation is simple.Plant stress-resistance agent of the present invention is gone back simultaneously
Sulphur required for plant (S) and sodium (Na) can be supplemented, has the function of foliar fertilizer.
Detailed description of the invention
Fig. 1 is the generation situation that embodiment 1 compares two groups of leaf surface waxes.
Fig. 2 is the volume diagram that NaHS reduces blade guard cell in embodiment 2.
Fig. 3 is the length and width and aperture figure that NaHS is substantially reduced guard cell in embodiment 2.
Fig. 4 is the stomatal frequency microgram for comparing processing group and control group in embodiment 3 under scanning electron microscope.
Fig. 5 is the stomatal frequency comparison diagram of processing group and control group in embodiment 3.
Specific embodiment
The present invention is further illustrated below by embodiment, but is not limited to following embodiment.
Embodiment 1:
Weigh a water NaHS (NaHSH2O) plus water prepares 50 μm of olL-1Aqueous solution, the treatment fluid be it is current existing
Match.Tomato, cucumber, tobacco and the arabidopsis of growth 4 weeks are watered with continuous drought after water, in the process by prepared NaHS water
Solution is stifling, smokes every other day once, 6 hours every time.The first most tender leaf is taken to fix after 10 days, film-making, scanning electron microscopic observation.
As shown in Figure 1, A is the upper lower epidermis of tomato；B is the upper lower epidermis of cucumber；C is the upper lower epidermis of tobacco；D is quasi-
The upper lower epidermis of southern mustard.Four kinds of materials divide control group (nature is handled without NaHS) and processing group, hold after being watered with water
Continuous arid.The NaHS of period processing group physiological concentration is stifling.The first leaf is taken to fix after 10 days, film-making, scanning electron microscopic observation.Knot
Fruit shows: obviously increasing by the processing group leaf surface waxes that NaHS is fumigated.
Embodiment 2.
Weigh a water NaHS (NaHSH2O) plus water prepares 80 μm of olL-1Aqueous solution, the treatment fluid be it is current existing
Match.Tomato, cucumber, tobacco and the arabidopsis of growth 4 weeks are watered with continuous drought after water, in the process by prepared NaHS water
Solution is stifling, smokes every other day once, 6 hours every time.The first most tender leaf is taken to fix after 10 days, film-making, scanning electron microscopic observation.
As shown in Fig. 2, A is the upper lower epidermis of tomato；B is the upper lower epidermis of cucumber；C is the upper lower epidermis of tobacco；D is quasi-
The upper lower epidermis of southern mustard.It was found that obviously becoming smaller by the processing group blade guard cell that NaHS is fumigated.
As shown in figure 3, A is tomato；B is cucumber；C is tobacco；D is arabidopsis, measures statistical by IMAGEJ software
Analysis, the results showed that in addition to tobacco, length and width, the air vent aperture of the guard cell of other three kinds of materials is significant after NaHS is stifling
Reduce.
Embodiment 3. weighs a water NaHS (NaHSH2O) plus water is configured to 100 μm of olL-1Aqueous solution, should
Treatment fluid is matching while using.Tomato, cucumber, tobacco and the arabidopsis of growth 4 weeks are watered with continuous drought after water, in the process will
Prepared NaHS aqueous solution is stifling, smokes every other day once, 6 hours every time.The first most tender leaf is taken to fix after 10 days, film-making is swept
Retouch Electronic Speculum observation.
As shown in figure 4, being the upper lower epidermis of four kinds of materials: A is epicuticle；B is lower epidermis；C is epicuticle；D is following table
Skin.It was found that being obviously reduced by the processing group Stoma of Leaves density that NaHS is fumigated.Statistical analysis, the results showed that, smoke abatement
Guard cell's number of three kinds of materials outside grass is substantially reduced after NaHS is stifling.
It can be seen from the above result that plant stress-resistance agent of the present invention can significantly inhibit the development of guard cell, promote blade table
The synthesis of skin wax achievees the effect that good enhancing plant resistant biology and abiotic stress.
Claims (4)
1. application of the NaHS as plant stress-resistance agent.
2. application according to claim 1, it is characterised in that: it is 5-500 μm of olL that NaHS, which is configured to concentration,-1Water
Solution.
3. application according to claim 2, it is characterised in that: it is 50-100 μm of olL that NaHS, which is configured to concentration,-1's
Aqueous solution.
4. application according to claim 2 or 3, it is characterised in that: NaHS is first configured to water-soluble by matching while using when use
Then liquid is sprayed on the mature leaf upper surface of plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811562357.3A CN109511674A (en) | 2018-12-20 | 2018-12-20 | Application of the NaHS as plant stress-resistance agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811562357.3A CN109511674A (en) | 2018-12-20 | 2018-12-20 | Application of the NaHS as plant stress-resistance agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109511674A true CN109511674A (en) | 2019-03-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811562357.3A Pending CN109511674A (en) | 2018-12-20 | 2018-12-20 | Application of the NaHS as plant stress-resistance agent |
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| Country | Link |
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| CN (1) | CN109511674A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447658A (en) * | 2019-09-11 | 2019-11-15 | 陕西科技大学 | NaHS is improving the application in corn seedling salt-resistance |
| CN111226983A (en) * | 2020-03-13 | 2020-06-05 | 陕西科技大学 | Application of NaHS in stabilizing monosaccharide content in cell wall of tomato seedling under cadmium ion stress |
| CN115363046A (en) * | 2022-07-22 | 2022-11-22 | 三亚南京农业大学研究院 | Application of sodium sulfide in crop weight-reducing and yield-increasing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210297A (en) * | 2011-06-04 | 2011-10-12 | 山西大学 | Plant antitranspirant and using method thereof |
| CN104584727A (en) * | 2015-01-22 | 2015-05-06 | 河南农业大学 | Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor |
-
2018
- 2018-12-20 CN CN201811562357.3A patent/CN109511674A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210297A (en) * | 2011-06-04 | 2011-10-12 | 山西大学 | Plant antitranspirant and using method thereof |
| CN104584727A (en) * | 2015-01-22 | 2015-05-06 | 河南农业大学 | Method for improving wheat drought resistance and salt stress resistance by taking sodium hydrosulfide as hydrogen sulfide donor |
Non-Patent Citations (1)
| Title |
|---|
| 王春语 等: ""植物蜡质研究进展"", 《辽宁农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447658A (en) * | 2019-09-11 | 2019-11-15 | 陕西科技大学 | NaHS is improving the application in corn seedling salt-resistance |
| CN111226983A (en) * | 2020-03-13 | 2020-06-05 | 陕西科技大学 | Application of NaHS in stabilizing monosaccharide content in cell wall of tomato seedling under cadmium ion stress |
| CN115363046A (en) * | 2022-07-22 | 2022-11-22 | 三亚南京农业大学研究院 | Application of sodium sulfide in crop weight-reducing and yield-increasing |
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Application publication date: 20190326 |
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