CN109507285A - A kind of device and method for realizing leakage blood monitoring with surface acoustic wave in blood purification system - Google Patents
A kind of device and method for realizing leakage blood monitoring with surface acoustic wave in blood purification system Download PDFInfo
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- CN109507285A CN109507285A CN201811245338.8A CN201811245338A CN109507285A CN 109507285 A CN109507285 A CN 109507285A CN 201811245338 A CN201811245338 A CN 201811245338A CN 109507285 A CN109507285 A CN 109507285A
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- microfluidic channel
- interdigital transducer
- blood
- acoustic wave
- surface acoustic
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/14—Dialysis systems; Artificial kidneys; Blood oxygenators ; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/22—Details, e.g. general constructional or apparatus details
- G01N29/222—Constructional or flow details for analysing fluids
Abstract
A kind of device and method for realizing leakage blood monitoring with surface acoustic wave in blood purification system, including waste liquid sample introduction hose, Murphy's burette, right drainage tube, first silica gel hose, first microfluidic channel entrance, left drainage tube, waste fluid bag, microfluidic platform, first microfluidic channel, first interdigital transducer, second interdigital transducer, second microfluidic channel, second microfluidic channel entrance, second silica gel hose, third microfluidic channel, first syringe pump, third interdigital transducer, third microfluidic channel entrance, third silica gel hose, second syringe pump, 4th interdigital transducer, driving testing circuit, microcontroller.This invention removes the influence of the uncertain factors such as bubble, environmental factor, the phenomenon that leaking blood false alarm is avoided.The high sensitivity of the reagent regulation and dependence surface acoustic wave itself injected by the second microfluidic channel meets different response demands in actual use so that leakage blood detection sensitivity can be adjusted arbitrarily.
Description
Technical field
The invention belongs to technical field of medical equipment, and specifically one kind is in blood purification system with surface acoustic wave reality
The now device and method of leakage blood monitoring.
Background technique
In the Medical Devices such as haemodialysis, washing, purification, the blood of patient is from internal outflow via hemodialysis equipment pipeline
The extracorporal circulatory system access of composition returns in vivo.It mainly include 3 bloody path, fluid path and monitoring system portions in this circulatory system
Point.Dialyzate enters dialyzer and the processes such as disperse, convection current, ultrafiltration occurs to blood samples of patients by dialysis membrane, removes patient's body
Extra harmful substance and moisture.
In blood purification process, if rupture of membranes occurs for filter, blood can be penetrated into waste liquid end by filter membrane, cause blood
Liquid leakage, to jeopardize patient vitals' safety.Therefore, any blood purification system requires leakage blood monitoring device to monitor in real time
Leakage blood situation in waste liquid is to guarantee safety when patient's treatment.
Traditional leakage blood monitoring method realizes that is, light emitting diode issues light beam using optical principle, passes through waste liquid
Optical signal transmissive is switched to electric signal by container, the light sensor circuit by being mounted on the waste fluid container other end, by comparing telecommunications
Number size to determine whether exist leakage blood phenomenon.Deep mixed due to ultrafiltrate color, light sensor circuit receives not
It is different with the light intensity signal of patient, easily lead to leakage blood false alarm.
Patent of invention " a kind of blood purification leakage blood monitoring system (CN201210257765.4) " is proposed using color
Sensor substitutes photosensitive sensor as transmission light receiving element.It is alleviated on certain procedures and uses photosensitive reception diode as connecing
The shortcomings that receiving transmission optical information, but the influence because of the uncertain factors such as presence of bubble in waste liquid can not be completely eliminated, and
It is influenced by sensor sensitivity response range itself, the blood in waste liquid, which will reach a certain amount of, can just be detected by a sensor.
Summary of the invention
The present invention provides a kind of device and method for realizing leakage blood monitoring with surface acoustic wave in blood purification system, to
Solve defect in the prior art.
The present invention is achieved by the following technical programs:
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system, including waste liquid sample introduction hose, not Fei Shi
Dropper, right drainage tube, the first silica gel hose, the first microfluidic channel entrance, left drainage tube, waste fluid bag, microfluidic platform,
One microfluidic channel, the first interdigital transducer, the second interdigital transducer, the second microfluidic channel, the second microfluidic channel enter and leave
Mouth, the second silica gel hose, third microfluidic channel, the first syringe pump, third interdigital transducer, third microfluidic channel enter and leave
Mouth, third silica gel hose, the second syringe pump, the 4th interdigital transducer, driving testing circuit, microcontroller, waste liquid sample introduction hose
It is connected with Murphy's burette, Murphy's burette is connected with right drainage tube, left drainage tube simultaneously, the other end of right drainage tube and first
One end of silica gel hose is connected, and the other end of the first silica gel hose is connected with the first microfluidic channel entrance, left drainage tube
The other end connects waste fluid bag, Murphy's burette is vertical and is higher than microfluidic platform placement, has in third microfluidic channel inlet
Where Three Faults Meet, third microfluidic channel is connected with the first microfluidic channel and the second microfluidic channel respectively by Where Three Faults Meet, three
The first interdigital transducer and the second interdigital transducer is arranged in the first microfluidic channel outlet both sides before prong, micro-fluidic in third
Before entrance and exit of the passage, the outlet both sides setting third interdigital transducer of third microfluidic channel and the 4th interdigital transducer, first
Microfluidic channel and third microfluidic channel are fixedly mounted on microfluidic platform, and the second microfluidic channel and third are micro-fluidic
Second microfluidic channel entrance, third microfluidic channel entrance corresponding to channel pass through the second silica gel hose, respectively
Three silica gel hoses are connected with the first syringe pump, the second syringe pump, and the first interdigital transducer, the second interdigital transducer, third are interdigital
Energy converter and the 4th interdigital transducer are connect with driving testing circuit, and driving testing circuit is connect with microcontroller.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, the miniflow
Control platform is divided into microchannel layers, piezoelectric layer and temperature control layer, and temperature sensor thermocouple is accompanied between piezoelectric layer and temperature control layer, temperature
Degree sensor thermocouple is located at below the first interdigital transducer.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, the piezoelectricity
Layer is lithium niobate (LiNbO3).
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, the temperature control
Layer is semiconductor chilling plate.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, the micro-control
Device processed controls constant-current source to drive temperature control layer, and thermocouple is connected with temperature measurement circuit, and temperature measurement circuit is connected with microcontroller.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, described first
Microfluidic channel, third microfluidic channel are snakelike.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, described second
Microfluidic channel is linear.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, described first
Surface acoustic wave direction caused by interdigital transducer and the second interdigital transducer, third interdigital transducer and the 4th interdigital transducer
It is vertical or angled with microfluidic channel liquid flow direction.
A kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, the driving
Detection circuit uses symmetric circuit, the first interdigital transducer, the second interdigital transducer, phase shifter, the first oscillation of amplifier composition
Circuit, third interdigital transducer and the 4th interdigital transducer, phase shifter, amplifier form the second oscillating circuit.
A kind of method for realizing leakage blood monitoring with surface acoustic wave in blood purification system as described above, including step packet
It includes:
A. 1:1 proportions ellagic acid reagent+0.025mol/L calcium chloride solution is pressed, and in 37 DEG C of water-bath pre-temperatures, will be matched molten
Liquid is packed into the first ejection of syringe pump syringe；
B. opening device power supply heats microfluidic platform, temperature is made to maintain 37 DEG C；
C. under the premise of blood purification system has worked, the second syringe pump is opened.Second injection pump work makes in " drawing " mode
Waste liquid flows into right drainage tube, the first microfluidic channel, third microfluidic channel from Murphy's burette, and finally flows into the second injection
Pump；The main function of second syringe pump is to prevent the insufficient pressure of blood purification system generation so that waste liquid passes through Murphy's burette
Into right drainage tube；Syringe volume used in second syringe pump regards waste liquid amount caused by a blood purification and determines；
D. the first syringe pump starts to inject ellagic acid+0.025mol/L calcium chloride solution proportion reagent to the second microfluidic channel,
Injection speed is adjustable, defaults flow velocity 3uL/s, and waste liquid flows through the reagent mixing of the first microfluidic channel and the second microfluidic channel
Third microfluidic channel is flowed into afterwards；
The frequency of oscillation for the first oscillating circuit that first interdigital transducer and the second interdigital transducer are constituted is as first is micro-fluidic
The variation of solution content in channel and change, the second oscillating circuit vibration that third interdigital transducer and the 4th interdigital transducer are constituted
Swinging frequency in addition to the influence of solution content, is also influenced by the second microfluidic channel reagent in by the first microfluidic channel；
When waste liquid is free of blood, because the solution content of the first microfluidic channel and the second microfluidic channel is stablized, the first oscillation
The frequency of oscillation of circuit and the second oscillating circuit is basicly stable, and fluctuation during which is cancelled out each other because of mixer action, therefore is mixed
Filtered frequency signal also tends towards stability；
When waste liquid when containing blood, the reagent phase that the solution of third microfluidic channel is flowed by blood and the second microfluidic channel is flowed into
Interaction causes the frequency of oscillation of the second oscillating circuit to change, so that the frequency signal after mixing and filtering is made to change,
The proportional relationship of frequency signal that blood content and microcontroller in waste liquid measure；
E. it by the signal frequency after measurement mixing and filtering, and is closed according to the ratio of blood content and frequency signal in waste liquid
System judges whether to be more than limit value, if being more than limit value, sending alarm signal.
The invention has the advantages that avoiding leakage blood this invention removes the influence of the uncertain factors such as bubble, environmental factor
The phenomenon that false alarm.The high sensitivity of the reagent regulation and dependence surface acoustic wave itself injected by the second microfluidic channel,
So that leakage blood detection sensitivity can be adjusted arbitrarily, meet different response demands in actual use.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is this hair
Bright some embodiments for those of ordinary skill in the art without any creative labor, can be with
It obtains other drawings based on these drawings.
Attached drawing 1 is that blood purification system leaks blood monitoring device figure；Attached drawing 2 is microfluidic platform sectional view；Attached drawing 3 is sound table
Surface wave driving testing circuit；Attached drawing 4 is microfluidic platform temperature control system.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
Every other embodiment obtained without creative efforts, shall fall within the protection scope of the present invention.
It is a kind of in blood purification system with surface acoustic wave realize leakage blood monitoring device, as shown, include waste liquid into
Sample hose 1, Murphy's burette 2, right drainage tube 3, the first silica gel hose 4, the first microfluidic channel entrance 5, left drainage tube 6,
Waste fluid bag 7, microfluidic platform 8, the first microfluidic channel 9, the first interdigital transducer 10, the second interdigital transducer 11, second are micro-
Flow control channel 12, the second microfluidic channel entrance 13, the second silica gel hose 14, third microfluidic channel 15, the first syringe pump
16, third interdigital transducer 17, third microfluidic channel entrance 18, third silica gel hose 19, the second syringe pump the 20, the 4th fork
Finger transducer 21, driving testing circuit 22, microcontroller 23, waste liquid sample introduction hose 1 and Murphy's burette 2 connect, not Fei Shi drop
Pipe 2 is connected with right drainage tube 3, left drainage tube 6 simultaneously, and the other end of right drainage tube 3 is connected with one end of the first silica gel hose 4,
The other end of first silica gel hose 4 is connected with the first microfluidic channel entrance 5, and the other end of left drainage tube 6 connects waste fluid bag
7, Murphy's burette 2 is vertical and is higher than the placement of microfluidic platform 8, has Where Three Faults Meet, third in 15 inlet of third microfluidic channel
Microfluidic channel 15 is connected with the first microfluidic channel 9 and the second microfluidic channel 12 respectively by Where Three Faults Meet, Where Three Faults Meet it
Preceding first microfluidic channel 9 exports both sides and the first interdigital transducer 10 and the second interdigital transducer 11 is arranged, micro-fluidic in third
Before entrance and exit of the passage 18, the outlet both sides setting third interdigital transducer 17 of third microfluidic channel 15 and the 4th interdigital transducer
21, the first microfluidic channel 9 is fixedly mounted on microfluidic platform 8 with third microfluidic channel 15, the second microfluidic channel
12 and third microfluidic channel 15 corresponding to the second microfluidic channel entrance 13, third microfluidic channel entrance 18 distinguish
It is connected by the second silica gel hose 14, third silica gel hose 19 with the first syringe pump 16, the second syringe pump 20, the first interdigital transducing
Device 10, the second interdigital transducer 11, third interdigital transducer 17 and the 4th interdigital transducer 21 are connect with driving testing circuit 22,
Driving testing circuit 22 is connect with microcontroller 23.This invention removes the influences of the uncertain factors such as bubble, environmental factor, keep away
The phenomenon that having exempted from leakage blood false alarm.The height of the reagent regulation and dependence surface acoustic wave itself injected by the second microfluidic channel
Sensitivity meets different response demands in actual use so that leakage blood detection sensitivity can be adjusted arbitrarily.
Specifically, as shown, microfluidic platform 8 described in the present embodiment divides for microchannel layers 30,31 and of piezoelectric layer
Temperature control layer 32, accompanies temperature sensor thermocouple 33 between piezoelectric layer 31 and temperature control layer 32, and temperature sensor thermocouple 33
In 10 lower section of the first interdigital transducer.
Specifically, as shown, piezoelectric layer 31 described in the present embodiment is lithium niobate (LiNbO3).
Further, as shown, temperature control layer 32 described in the present embodiment is semiconductor chilling plate.
Further, as shown, microcontroller 23 described in the present embodiment controls constant-current source 51 to drive temperature control layer
32, thermocouple 33 and temperature measurement circuit 50 connect, and temperature measurement circuit 50 and microcontroller 23 connect.The temperature of microfluidic platform 8 passes through
The temperature of the reading microfluidic platform 8 of temperature measurement circuit 50.
Further, as shown, the first microfluidic channel 9, third microfluidic channel 15 described in the present embodiment are
It is snakelike.
Further, as shown, the second microfluidic channel 12 described in the present embodiment is linear.
Further, as shown, the first interdigital transducer 10 and the second interdigital transducer 11 described in the present embodiment,
Surface acoustic wave direction caused by third interdigital transducer 17 and the 4th interdigital transducer 21 and microfluidic channel liquid flowing side
To vertical or angled.
Further, as shown, driving testing circuit 22 described in the present embodiment uses symmetric circuit, first is interdigital
Energy converter 10, the second interdigital transducer 11, phase shifter 40, amplifier 41 form the first oscillating circuit 46, third interdigital transducer
17 and the 4th interdigital transducer 21, phase shifter 42, amplifier 43 form the second oscillating circuit 47,.First oscillating circuit 46 and
Two oscillating circuits 47 are mixed by multiplier 44, and filter out high-frequency signal, the low frequency of output by low-pass filter (LPF) 45
Signal is obtained a result by microcontroller 23 by frequency measurement.
As shown in Figure 1, blood purification system leakage blood monitoring device of the invention includes: waste liquid sample introduction hose 1, not Fei Shi drop
It is pipe 2, right drainage tube 3, the first silica gel hose 4, the first microfluidic channel entrance 5, left drainage tube 6, waste fluid bag 7, micro-fluidic flat
Platform 8, the first microfluidic channel 9, the first interdigital transducer 10, the second interdigital transducer 11, the second microfluidic channel 12, second are micro-
Flow control entrance and exit of the passage 13, the second silica gel hose 14, third microfluidic channel 15, the first syringe pump 16, third interdigital transducer
17, third microfluidic channel entrance 18, third silica gel hose 19, the second syringe pump 20, the 4th interdigital transducer 21, driving inspection
Slowdown monitoring circuit 22, microcontroller 23.
The waste liquid sample introduction hose 1 and Murphy's burette 2 connect, Murphy's burette 2 while and right drainage tube 3, Zuo Yinliu
Pipe 6 connects.The waste liquid for flowing into Murphy's burette 2 from waste liquid sample introduction hose 1 separately flows into right drainage tube 3 and left drainage tube 6.It flows into
The waste liquid of left drainage tube 6 is introduced to waste fluid bag 7.It flows into the useless of right drainage tube 3 and microfluidic platform is entered by the first silica gel hose 4
The first microfluidic channel entrance 5 where 8.Murphy's burette 2 is vertical and is higher than the placement of microfluidic platform 8.
As shown in Fig. 2, the microfluidic platform 8 divides three layers: microchannel layers 30, piezoelectric layer 31 and temperature control layer 32.Piezoelectric layer
31 be lithium niobate (LiNbO3), but is not limited to lithium niobate.Temperature control layer 32 is semiconductor chilling plate.In piezoelectric layer 31 and temperature control layer 32
Between, temperature sensor thermocouple 33 is accompanied below the first interdigital transducer 10.
As shown in Figure 1, first microfluidic channel 9, third microfluidic channel 15 are snakelike but unlimited in microchannel layers 30
In snakelike.Second microfluidic channel 12 is linear, but is not limited to linear.First microfluidic channel 9 and third are micro-fluidic logical
15 diameter range of road is 1 μm~3mm.Have Where Three Faults Meet in 15 inlet of third microfluidic channel, respectively with the first microfluidic channel 9
It is connected with the second microfluidic channel 12.There is the first interdigital transducer 10 on the first microfluidic channel 9 outlet both sides before Where Three Faults Meet
With the second interdigital transducer 11.Before third microfluidic channel entrance 18, there is 17 He of third interdigital transducer on microchannel both sides
4th interdigital transducer 21.
Further, step described in the present embodiment includes:
A. 1:1 proportions ellagic acid reagent+0.025mol/L calcium chloride solution is pressed, and in 37 DEG C of water-bath pre-temperatures, will be matched molten
Liquid is packed into 16 injection needle tube of the first syringe pump；
B. opening device power supply heats microfluidic platform 8, temperature is made to maintain 37 DEG C；
C. under the premise of blood purification system has worked, the second syringe pump 20 is opened.Second syringe pump 20 works in " drawing " mould
Formula makes waste liquid flow into right drainage tube 3, the first microfluidic channel 9, third microfluidic channel 15 from Murphy's burette 2, and finally flows
Enter the second syringe pump 20；The main function of second syringe pump 20 is to prevent the insufficient pressure of blood purification system generation so that waste liquid
Enter right drainage tube 3 by Murphy's burette 2；Syringe volume used in second syringe pump 20 is produced depending on a blood purification
Raw waste liquid amount determines；
D. the first syringe pump 16 starts to inject ellagic acid+0.025mol/L calcium chloride solution with having a competition to the second microfluidic channel 12
Agent, injection speed is adjustable, defaults flow velocity 3uL/s, and waste liquid flows through the examination of the first microfluidic channel 9 and the second microfluidic channel 12
Third microfluidic channel 15 is flowed into after agent mixing；
The frequency of oscillation for the first oscillating circuit 46 that first interdigital transducer 10 and the second interdigital transducer 11 are constituted is with first
The variation of solution content in microfluidic channel 9 and change, third interdigital transducer 17 and the 4th interdigital transducer 21 constitute the
It is also micro-fluidic by second in addition to two oscillating circuits, 47 frequency of oscillation solution content in by the first microfluidic channel 9 is influenced
The influence of 12 reagent of channel；
When waste liquid is free of blood, because the solution content of the first microfluidic channel 9 and the second microfluidic channel 12 is stablized, the first vibration
The frequency of oscillation for swinging circuit 46 and the second oscillating circuit 47 is basicly stable, and fluctuation during which is cancelled out each other because of mixer action, because
Frequency signal after this mixing and filtering also tends towards stability；
When waste liquid when containing blood, the examination that the solution of third microfluidic channel 15 is flowed by blood and the second microfluidic channel 12 is flowed into
Agent interaction, causes the frequency of oscillation of the second oscillating circuit 47 to change, so that the frequency signal after mixing and filtering be made to send out
The proportional relationship of frequency signal raw to change, that blood content and microcontroller in waste liquid measure；
E. it by the signal frequency after measurement mixing and filtering, and is closed according to the ratio of blood content and frequency signal in waste liquid
System judges whether to be more than limit value, if being more than limit value, sending alarm signal.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: it still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features；
And these are modified or replaceed, technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution spirit and
Range.
Claims (10)
1. it is a kind of in blood purification system with surface acoustic wave realize leakage blood monitoring device, it is characterised in that: including waste liquid into
Sample hose (1), Murphy's burette (2), right drainage tube (3), the first silica gel hose (4), the first microfluidic channel entrance (5),
Left drainage tube (6), waste fluid bag (7), microfluidic platform (8), the first microfluidic channel (9), the first interdigital transducer (10), second
Interdigital transducer (11), the second microfluidic channel (12), the second microfluidic channel entrance (13), the second silica gel hose (14),
Third microfluidic channel (15), the first syringe pump (16), third interdigital transducer (17), third microfluidic channel entrance
(18), third silica gel hose (19), the second syringe pump (20), the 4th interdigital transducer (21), driving testing circuit (22), micro-control
Device (23) processed, waste liquid sample introduction hose (1) and Murphy's burette (2) connection, Murphy's burette (2) while and right drainage tube (3), a left side
Drainage tube (6) connection, the other end of right drainage tube (3) are connected with one end of the first silica gel hose (4), the first silica gel hose (4)
The other end be connected with the first microfluidic channel entrance (5), the other end of left drainage tube (6) connects waste fluid bag (7), Mo Fei
Family name's dropper (2) is vertical and is higher than microfluidic platform (8) placement, has Where Three Faults Meet, third in third microfluidic channel (15) inlet
Microfluidic channel (15) is connected with the first microfluidic channel (9) and the second microfluidic channel (12) respectively by Where Three Faults Meet, three
First microfluidic channel (9) outlet both sides setting the first interdigital transducer (10) and the second interdigital transducer (11) before prong,
Before third microfluidic channel entrance (18), third interdigital transducer is arranged in the outlet both sides of third microfluidic channel (15)
(17) and the 4th interdigital transducer (21), the first microfluidic channel (9) are fixedly mounted on micro- with third microfluidic channel (15)
On flow control platform (8), the second microfluidic channel corresponding to the second microfluidic channel (12) and third microfluidic channel (15) goes out
Entrance (13), third microfluidic channel entrance (18) respectively by the second silica gel hose (14), third silica gel hose (19) with
First syringe pump (16), the second syringe pump (20) are connected, the first interdigital transducer (10), the second interdigital transducer (11), third
Interdigital transducer (17) and the 4th interdigital transducer (21) are connect with driving testing circuit (22), driving testing circuit (22) with it is micro-
Controller (23) connection, microfluidic platform (8) is divided into microchannel layers (30), piezoelectric layer (31) and temperature control layer (32), in piezoelectric layer
(31) it is accompanied between temperature control layer (32) temperature sensor thermocouple (33), it is interdigital that temperature sensor thermocouple (33) is located at first
Below energy converter (10).
2. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: the piezoelectric layer (31) is lithium niobate (LiNbO3).
3. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: the temperature control layer (32) is semiconductor chilling plate.
4. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: the microcontroller (23) controls constant-current source (51) to drive temperature control layer (32), and thermocouple (33) and thermometric are electric
Road (50) connection, temperature measurement circuit (50) and microcontroller (23) connection.
5. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: first microfluidic channel (9), third microfluidic channel (15) are snakelike.
6. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: second microfluidic channel (12) is linear.
7. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
It is characterized in that: first interdigital transducer (10) and the second interdigital transducer (11), third interdigital transducer (17) and
Surface acoustic wave direction caused by four interdigital transducers (21) is vertical or at an angle with microfluidic channel liquid flow direction.
8. a kind of device for realizing leakage blood monitoring with surface acoustic wave in blood purification system according to claim 1,
Be characterized in that: the driving testing circuit (22) uses symmetric circuit, the first interdigital transducer (10), the second interdigital transducer
(11), phase shifter (40), amplifier (41) composition the first oscillating circuit (46), third interdigital transducer (17) and the 4th interdigital change
Energy device (21), phase shifter (42), amplifier (43) composition the second oscillating circuit (47),.
9. a kind of method for realizing leakage blood monitoring with surface acoustic wave in blood purification system, it is characterised in that: comprising steps of
A. 1:1 proportions ellagic acid reagent+0.025mol/L calcium chloride solution is pressed, and in 37 DEG C of water-bath pre-temperatures, will be matched molten
Liquid is packed into the first syringe pump (16) injection needle tube；
B. opening device power supply heats microfluidic platform (8), temperature is made to maintain 37 DEG C；
C. it under the premise of blood purification system has worked, opens the second syringe pump (20).
10. the second syringe pump (20) work makes waste liquid flow into right drainage tube (3), first from Murphy's burette (2) in " drawing " mode
Microfluidic channel (9), third microfluidic channel (15), and finally flow into the second syringe pump (20)；The master of second syringe pump (20)
Act on is the insufficient pressure for preventing blood purification system from generating so that waste liquid enters right drainage tube by Murphy's burette (2)
(3)；Syringe volume used in second syringe pump (20) regards waste liquid amount caused by a blood purification and determines；
D. the first syringe pump (16) to the second microfluidic channel (12) start inject ellagic acid+0.025mol/L calcium chloride solution match
Than reagent, injection speed is adjustable, defaults flow velocity 3uL/s, and waste liquid flows through the first microfluidic channel (9) and the second microfluidic channel
(12) third microfluidic channel (15) are flowed into after reagent mixing；
The frequency of oscillation for the first oscillating circuit (46) that first interdigital transducer (10) and the second interdigital transducer (11) are constituted with
In the first microfluidic channel (9) variation of solution content and change, third interdigital transducer (17) and the 4th interdigital transducer
(21) in addition to the second oscillating circuit (47) frequency of oscillation constituted solution content in by the first microfluidic channel (9) is influenced,
Also influenced by the second microfluidic channel (12) reagent；
When waste liquid is free of blood, because the solution content of the first microfluidic channel (9) and the second microfluidic channel (12) is stablized, the
The frequency of oscillation of one oscillating circuit (46) and the second oscillating circuit (47) is basicly stable, and fluctuation during which is mutual because of mixer action
It offsets, therefore the frequency signal after mixing and filtering also tends towards stability；
When waste liquid when containing blood, the solution of third microfluidic channel (15) is flowed into because blood and the second microfluidic channel (12) flow into
Reagent interaction, cause the frequency of oscillation of the second oscillating circuit (47) to change, to make the frequency after mixing and filtering
Signal changes, the proportional relationship of frequency signal that the blood content and microcontroller in waste liquid measure；
E. it by the signal frequency after measurement mixing and filtering, and is closed according to the ratio of blood content and frequency signal in waste liquid
System judges whether to be more than limit value, if being more than limit value, sending alarm signal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811245338.8A CN109507285B (en) | 2018-10-24 | 2018-10-24 | Device and method for monitoring blood leakage in blood purification system by using surface acoustic waves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN111815700A (en) * | 2020-01-11 | 2020-10-23 | 浙江师范大学 | Device and method for measuring mechanical characteristics of red blood cells |
| CN113318281A (en) * | 2021-04-28 | 2021-08-31 | 四川省肿瘤医院 | Drainage bottle alarm device |
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| CN109507285B (en) | 2021-01-26 |
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