CN109504684A - The application of Ran gene and its dsRNA in control of insect - Google Patents
The application of Ran gene and its dsRNA in control of insect Download PDFInfo
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- CN109504684A CN109504684A CN201811563907.3A CN201811563907A CN109504684A CN 109504684 A CN109504684 A CN 109504684A CN 201811563907 A CN201811563907 A CN 201811563907A CN 109504684 A CN109504684 A CN 109504684A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
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Abstract
The present invention provides a kind of application of insect Ran full length gene cDNA sequence and its dsRNA in control of insect.Specifically by bioinformatics method, the Ran full length gene cDNA sequence that sequence is SEQ ID NO:1 is obtained from migratory locusts transcript profile database.According to SEQ ID NO:1, design and synthesize the dsRNA of the gene, inject after entering migratory locusts body cavity can specificity silencing target gene, so that so that migratory locusts growth and development is obstructed leads to death.Many experiments show that its lethality reaches 100%.It due to specific and efficient lethality of the invention, has important practical significance for control of insect, new approach can be provided for control of insect.
Description
Technical field
The present invention relates to biotechnology and Agricultural pest control fields.More particularly to migratory locusts Ran gene and its dsRNA in evil
Application in worm prevention and treatment.
Background technique
Migratory locustsLocusta migratoria, it is a kind of agricultural pests of ntercontinental, is distributed mainly on Asia, Europe, non-, Australia four
Continent.It has the characteristics that fulminant, gregariousness and migrating property, once occurring, is directed not only to wide, and breaks with tremendous force, cause
Calamity is serious.At present in locust control work, still using chemical insecticide as main means, a large amount of uses of chemical insecticide, no
Only insect is easy to cause to develop drug resistance, and brings serious pollution to ecological environment, also constituted a threat to the health of the mankind, because
This researches and develops green new pesticide and is of great significance to China's prevention and treatment locust work.
The phenomenon that RNA interference (RNAi) is gene silencing after a kind of specific transcriptional as caused by double stranded rna molecule, from
Since the acquisition Nobel Prizes in 2006, RNAi technology ranks among always Environment Science.RNAi is not only having for research gene function
Power tool, while also there is very high potential in terms of control of insect.It is had a characteristic that by RNAi progress control of insect and 1) is killed
Worm has specificity, to non-target organism without lethal effect；2) RNA is easily degraded in nature, noresidue；3) nontoxic to environment
It is harmless, it is comparatively safe.The control of insect based on RNAi forth generation insecticide has been known as in the world at present.Screening and identification are to evil
The vital gene of worm growth and development is using the key link of RNAi technology exploitation novel pesticide, because not every
DsRNA can effective silencing of target genes expression, nor all dsRNA can kill off the insect pests
Ran is one of small G protein family member, can be converted between two kinds of forms of RanGDP and RanGTP, in regulation core
It plays an important role in the assembling of nuclear membrane after matter transport, micro-pipe are formed, mitotic spindle is formed and cell division.It adopts
With RNA perturbation technique, appearance 100% is lethal existing after this seminar confirms injection migratory locusts Ran gene dsRNA to 5 age nymphs for the first time
As.Therefore, Ran gene can be used as the lethal target gene of height of the control of insect technology based on RNAi.
Summary of the invention
The answering in lethal migratory locusts it is an object of the invention to provide a kind of insect Ran full length gene cDNA sequence and its dsRNA
With.
The present invention provides a kind of migratory locusts Ran full length gene cDNA sequence, and nucleotides sequence is classified as SEQ ID NO:1.The base
Because full length cDNA sequence is obtained based on migratory locusts transcript profile database search, which is 648 bp, nucleotides sequence
It is classified as SEQ ID NO:1.
The present invention provides application of the migratory locusts Ran gene dsRNA in control of insect, is based on migratory locusts Ran gene cDNA sequence,
The upstream primer and downstream primer for containing T7 promoter by 5.0 software design of primer premier, are obtained by PCR amplification
Obtain the template that both ends are T7 promoter.According to T7 RiboMAX Express RNAi System after kits
(Promega) kit illustrates that synthesis dsRNA is transcribed in vitro.The dsRNA injection of synthesis is entered into migratory locusts by micro syringe
In body cavity.The result shows that: the mRNA of migratory locusts Ran gene is expressed and is significantly reduced after injection dsRNA, and migratory locusts growth and development occur and are obstructed
And cause 100% death.
Detailed description of the invention
Fig. 1: the dsRNA of injection Ran gene enters in 5 age migratory locusts bodies after 24 h, 48 h and 72 h to Ran gene mRNA
The influence of expression.β-actin is reference gene.Wherein *P< 0.05, * * * *P<0.001。
Fig. 2: the dsRNA of the injection Ran gene influence to the migratory locusts nymph growth and development of 5 ages.Inject the dsRNA of Ran gene
There is growth and development and are obstructed and lead to 100% death in migratory locusts.A: left side is the control of injection water, and right side is injection SEQ ID
The dsRNA, i.e. ds of NO:2 synthesisLmRan。
Specific embodiment
Embodiment one: the acquisition of migratory locusts Ran full length gene cDNA and genetic fragment
Transcript profile database based on migratory locusts scans for migratory locusts Ran gene using bioinformatics method, obtains migratory locusts
Ran gene (LmRan) full length cDNA sequence, using 5.0 software design upstream and downstream primer of primer premier for verifying
Full length cDNA sequence, and it is sent to the synthesis of Shanghai Invitrogen biology Co., Ltd.Choose healthy growth, half male and half female in the same size
5 age migratory locusts nymphs 3, by under its body wall fast anatomical under Stereo microscope, and be frozen in liquid nitrogen.According to TaKaRa
RNAiso Plus kit extracts RNA.Using Reverse Transcriptase M-MLV (RNase H-) specification by institute
RNA reverse transcription is proposed into the first chain cDNA, PCR amplification is passed through in conjunction with the upstream and downstream primer of design as template with thisLmRanBase
Because of full length sequence.Obtained product is purified, Cloning Transformation is into Escherichia coli, and be sent to Shanghai Invitrogen biology and have
Limit company is sequenced, and sequence is SEQ ID NO:1.
Embodiment two: migratory locusts Ran gene specific dsRNA synthesis
1) design of migratory locusts Ran gene dsRNA primer
Based on migratory locusts Ran full length gene cDNA sequence, using 5.0 software design dsRNA primer of primer premier, sequence
Column are respectively SEQ ID NO:3 and SEQ ID NO:4.Upstream and downstream primer carries T7 promoter sequence.All primers are by upper
The synthesis of extra large Invitrogen biology Co., Ltd.
2) synthesis of migratory locusts Ran gene dsRNA
Plasmid is extracted as template using the Ran gene that above-mentioned sequence is SEQ ID NO:1, and SEQ ID NO:3 and SEQ ID NO:4 is done
For upstream and downstream primer, PCR amplification is carried out.By the PCR product of amplification, sequence is SEQ ID NO:2, through FastPure Gel
DNA Extraction Mini Kit(Vazyme) after kits according to T7 RiboMAX Express RNAi
System(Promega) kit illustrates that synthesis dsRNA is transcribed in vitro.Use NANODROP 2000(Thermo
Scientific it) is quantified, its final concentration is made to reach 2 μ g/ μ L.It is spare to be stored in -80 DEG C of super low temperature refrigerators.
Embodiment three: the lethal migratory locusts experiment of migratory locusts Ran gene dsRNA
1, the injection of migratory locusts Ran gene dsRNA
Choosing healthy growth, in the same size, half male and half female, totally 60 2 days 5 ages nymphs carry out experimental group dsLmRanThe note of gene
It penetrates.By the ds of synthesisLmRanUsing micro syringe by 5 μ L(10 μ g dsLmRan) gently inject into nymph flank portion
Two, between three uromeres.Same volume ponding is injected to control group (60, half male and half female) simultaneously.Migratory locusts after injection are placed in 30 DEG C
Raised in constant temperature biochemical cultivation case (illumination: interlunation=14 h:10 h, 30 ± 2 DEG C of temperature, humidity 60%), feed daily
Fresh wheat seedling and wheat bran.
2, migratory locusts Ran gene silencing detects
Injection water and ds are collected respectivelyLmRan24 h, 48 h and 72 h teleonymph polypides carry out Total RNAs extraction, each time point
Control and injection dsLmRanEach 3 biology of group repeats, and each biology repeats 3 test worms, and reverse transcription is at the first chain
CDNA, using RT-qPCR method difference testing goal gene (LmRan) and house-keeping gene (β-actin) relative expression quantity, from
And its silence efficiency is calculated.The result shows that compared with the control group, injecting dsLmRanGroup test wormLmRanGene expression is aobvious
Writing reduces (as shown in Figure 1).
3,5 age nymph Phenotypic Observations after injection Ran gene dsRNA
5 age nymphs inject dsLmRanAfterwards, control group polypide was all successfully casted off a skin at 7 days to adult, and Adult Development shape after husking
State is good.Compared with the control group, ds is injectedLmRanMigratory locusts do not occur husking delay phenomenon afterwards, all dead at the 7th day, dead
Rate reaches 100%, as shown in Figure 2.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
It is familiar with those skilled in the art in the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of, should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Sequence table
<110>University Of Shanxi
<120>application of Ran gene and its dsRNA in control of insect
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 648
<212> DNA
<213>migratory locusts (Locusta migratoria)
<400> 1
atggctcaag aagctgatat gcctgcgttt aagtgtgtcc ttgttgggga tggaggcaca 60
ggaaaaacca catttgtgaa aaggcattta actggcgaat ttgaaaagaa atatgtcgcc 120
acacttggcg tggaagtaca tccgctggtg ttccacacta acagaggtgc aattcgcttt 180
aatgtttggg atactgctgg acaggaaaaa ttcggaggac tgagagatgg ttactatatt 240
cagggccaat gcgcaattat aatgtttgat gtcacatcac gtgttacata caagaatgta 300
cccaactggc atagagacct tgtacgtgtc tgtgaaaata tcccaatagt tctttgtgga 360
aataaagttg acattaagga ccgaaaagtt aaagcaaaaa gcatcgtttt ccataggaag 420
aagaatttgc agtattatga catcagtgca aagagcaact acaactttga aaagcctttc 480
ttgtggttag ctagaaagct aattggtgac cctaatctgg agtttgttgc tatgcctgct 540
cttgtgcctc ctgaggtgac aatggaccca acatggcaac aacaaataga aaatgatctg 600
aaggaagcat cagagacggc tttgcctgaa gacgatgaag acctgtaa 648
<210> 2
<211> 508
<212> DNA
<213>migratory locusts (Locusta migratoria)
<400> 2
atggctcaag aagctgatat gcctgcgttt aagtgtgtcc ttgttgggga tggaggcaca 60
ggaaaaacca catttgtgaa aaggcattta actggcgaat ttgaaaagaa atatgtcgcc 120
acacttggcg tggaagtaca tccgctggtg ttccacacta acagaggtgc aattcgcttt 180
aatgtttggg atactgctgg acaggaaaaa ttcggaggac tgagagatgg ttactatatt 240
cagggccaat gcgcaattat aatgtttgat gtcacatcac gtgttacata caagaatgta 300
cccaactggc atagagacct tgtacgtgtc tgtgaaaata tcccaatagt tctttgtgga 360
aataaagttg acattaagga ccgaaaagtt aaagcaaaaa gcatcgtttt ccataggaag 420
aagaatttgc agtattatga catcagtgca aagagcaact acaactttga aaagcctttc 480
ttgtggttag ctagaaagct aattggtg 508
<210> 3
<211> 38
<212> DNA
<213>migratory locusts (Locusta migratoria)
<400> 3
taatacgact cactataggg atggctcaag aagctgat 38
<210> 4
<211> 39
<212> DNA
<213>migratory locusts (Locusta migratoria)
<400> 4
taatacgact cactataggg caccaattag ctttctagc 39
Claims (6)
1. a kind of migratory locusts Ran gene, it is characterized in that nucleotides sequence is classified as the sequence of SEQ ID NO:1.
2. a kind of migratory locusts Ran genetic fragment, it is characterized in that nucleotides sequence is classified as the sequence of SEQ ID NO:2.
3. a kind of dsRNA of migratory locusts Ran genetic fragment SEQ ID NO:2 synthesis as claimed in claim 2.
4. application of the dsRNA of migratory locusts Ran gene as claimed in claim 3 in migratory locusts prevent and treat.
5. application of the dsRNA of migratory locusts Ran gene as claimed in claim 3 in migratory locusts prevent and treat, it is characterised in that: described
DsRNA is prepared into spray-type insecticide, and perhaps dsRNA is prepared into bait formulation or dsRNA is transferred to migratory locusts feeding plant body.
6. the preparation method of dsRNA as claimed in claim 3, step are as follows: the upstream primer SEQ designed according to SEQ ID NO:1
ID NO:3 and downstream primer SEQ ID NO:4 obtains SEQ ID NO:2 by PCR amplification, and product contains T7 promoter；It produces
According to T7 RiboMAX Express RNAi System(Promega after object is purified) kit illustrate be transcribed in vitro synthesis
dsRNA。
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| CN109504684B CN109504684B (en) | 2021-07-27 |
Family
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937822A (en) * | 2014-02-28 | 2014-07-23 | 山西大学 | Sequence of locust I type chitinase gene, and application of dsRNA of locust I chitinase gene |
| CN104630247A (en) * | 2015-02-13 | 2015-05-20 | 山西大学 | Insect chitin deacetylate enzyme genes 1 and application of insect chitin deacetylate enzyme genes 1 in pest control |
| CN104774843A (en) * | 2015-04-07 | 2015-07-15 | 山西大学 | Application of Knickkopf gene dsRNA in pest control |
| CN104789571A (en) * | 2015-04-07 | 2015-07-22 | 山西大学 | Retroactive gene and application of dsRNA (double-stranded ribonucleic acid) of Retroactive gene in pest control |
| CN105695476A (en) * | 2016-03-29 | 2016-06-22 | 山西大学 | Migratory locust wing epidermis protein gene and application thereof in pest control |
| CN105755006A (en) * | 2016-03-29 | 2016-07-13 | 山西大学 | Migratory locust wing specific cuticle protein gene and application of dsRNA thereof |
| WO2017176813A1 (en) * | 2016-04-04 | 2017-10-12 | University Of Florida Research Foundation, Incorporated | Manipulation of eif3 to modulate repeat associated non-atg (ran) translation |
| WO2018026992A1 (en) * | 2016-08-03 | 2018-02-08 | Monsanto Technology Llc | Compositions and methods for controlling mosquitoes |
| CN107699569A (en) * | 2017-11-26 | 2018-02-16 | 山西大学 | Migratory locusts coria epidermal protein gene 1 and its application in locust control |
| CN107988231A (en) * | 2017-11-26 | 2018-05-04 | 山西大学 | Migratory locusts coria epidermal protein gene 6 and its application in locust control |
| WO2018195110A1 (en) * | 2017-04-17 | 2018-10-25 | University Of Florida Research Foundation, Incorporated | Regolation of ran translation by pkr and eif2a-p pathways |
| CN110172463A (en) * | 2018-12-20 | 2019-08-27 | 山西大学 | Application of the migratory locusts Knickkopf 3-5 ' gene dsRNA in control of insect |
-
2018
- 2018-12-20 CN CN201811563907.3A patent/CN109504684B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937822A (en) * | 2014-02-28 | 2014-07-23 | 山西大学 | Sequence of locust I type chitinase gene, and application of dsRNA of locust I chitinase gene |
| CN104630247A (en) * | 2015-02-13 | 2015-05-20 | 山西大学 | Insect chitin deacetylate enzyme genes 1 and application of insect chitin deacetylate enzyme genes 1 in pest control |
| CN104774843A (en) * | 2015-04-07 | 2015-07-15 | 山西大学 | Application of Knickkopf gene dsRNA in pest control |
| CN104789571A (en) * | 2015-04-07 | 2015-07-22 | 山西大学 | Retroactive gene and application of dsRNA (double-stranded ribonucleic acid) of Retroactive gene in pest control |
| CN105695476A (en) * | 2016-03-29 | 2016-06-22 | 山西大学 | Migratory locust wing epidermis protein gene and application thereof in pest control |
| CN105755006A (en) * | 2016-03-29 | 2016-07-13 | 山西大学 | Migratory locust wing specific cuticle protein gene and application of dsRNA thereof |
| WO2017176813A1 (en) * | 2016-04-04 | 2017-10-12 | University Of Florida Research Foundation, Incorporated | Manipulation of eif3 to modulate repeat associated non-atg (ran) translation |
| WO2018026992A1 (en) * | 2016-08-03 | 2018-02-08 | Monsanto Technology Llc | Compositions and methods for controlling mosquitoes |
| WO2018195110A1 (en) * | 2017-04-17 | 2018-10-25 | University Of Florida Research Foundation, Incorporated | Regolation of ran translation by pkr and eif2a-p pathways |
| CN107699569A (en) * | 2017-11-26 | 2018-02-16 | 山西大学 | Migratory locusts coria epidermal protein gene 1 and its application in locust control |
| CN107988231A (en) * | 2017-11-26 | 2018-05-04 | 山西大学 | Migratory locusts coria epidermal protein gene 6 and its application in locust control |
| CN110172463A (en) * | 2018-12-20 | 2019-08-27 | 山西大学 | Application of the migratory locusts Knickkopf 3-5 ' gene dsRNA in control of insect |
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