CN109504625A - Microbial bacterial agent and the application of one plant of Bacillus cercus DIF1 and its production - Google Patents
Microbial bacterial agent and the application of one plant of Bacillus cercus DIF1 and its production Download PDFInfo
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- CN109504625A CN109504625A CN201811399378.8A CN201811399378A CN109504625A CN 109504625 A CN109504625 A CN 109504625A CN 201811399378 A CN201811399378 A CN 201811399378A CN 109504625 A CN109504625 A CN 109504625A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/085—Bacillus cereus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
Abstract
The invention discloses one plant of Bacillus cercus DIF1 and its microbial bacterial agent and the application of production, which is preserved in China typical culture collection center on May 15th, 2018, and deposit number is CCTCC NO:M2018274.Bacillus cercus DIF1 of the present invention has the restoring function of stronger dissimilatory reduction Fe (III), Dissolvable Fe (III) and solid-state Fe (III) can efficiently be restored, meanwhile this plant of Bacillus cercus DIF1 has stronger hexavalent chrome reduction ability and electricity generation ability.Present invention discloses the application studies in terms of the new property of Bacillus cercus and its function, and new material is provided in terms of the improvement of environmental contaminants for dissimilatory iron reduction bacterium.
Description
Technical field
The present invention relates to environmental organism reparation more particularly to one plant of Bacillus cercus DIF1 and its microbial bacteria of production
Agent and application.
Background technique
Iron is that most wide one of element is distributed on the earth, is the high element of abundance the 4th in the earth's crust, is that heavy metal transformation turns
One of the geological process and the key factor of environmental change such as change and the migration of other organic pollutants.Microorganism is earth's surface ferrikinetics
Core participant, by bacterium it is extracellular occur iron oxidation or reduction reaction characterized by dissimilation be ferrikinetics important driving
Power.Earliest microbial metabolism is likely to be by the microorganism alienation Fe (III) of terminal electron acceptor reduction of extracellular ferriferous oxide
Form.
Dissimilatory iron reduction bacterium refers to that one kind can be reduced, simultaneously using Fe (III) as sole electron acceptor, Fe (III)
Organic carbon source is aoxidized, and therefrom obtains energy for the general designation of a quasi-microorganism of own growth.Dissimilatory iron reduction bacterium exhales in bacterium
It inhales and various metals ion can be restored in growth course, remove organic matter and heavy metal, it will by extracellular electronics transfer mechanism
The electron transmission of organic metabolism intracellular oxidizes metal object reduction to extracellular electron acceptor, such as solid metal oxide.
Thus, dissimilatory iron reduction bacterium metallic element geochemical cycle and the energy in terms of play a significant role.
Pollution of chromium is presently the most universal one of heavy metal contaminants, and certain density Cr (VI) meeting life-threatening is strong
Health.Chromium is mainly used for the industries such as intermetallic composite coating, plating, process hides, and chromate is commonly incorporated into industrial processes to prevent recirculated water
Corrosion to equipment.And the waste water and exhaust gas of industrial department discharge, it is the artificial source of chromium in environment.Although in industrial wastewater
Chromium is mainly rendered as trivalent form, but is discharged into the trivalent chromium in natural environment to be transformed into toxicity under the influence of environment stronger
Cr VI.For chromium generally with the presence of the form of Cr (VI) and Cr (III), Cr (VI) has very strong mobility, long in water body environment
Phase is exposed in Cr VI environment can slow poisoning initiation cancer.By Cr, ((VI), which is reduced to Cr (III), can reduce toxicity.Reduction
The method of chromium has electronation, absorption, ion exchange, UF membrane, biological prosthetic and electrochemical rehabilitation.Wherein bioremediation technology
A kind of means without secondary pollution are provided for pollution of chromium area.
Summary of the invention
Goal of the invention: it is an object of that present invention to provide one plant of Bacillus cercus DIF1, have to iron ion and chromium ion
Have reduction, can be applied to iron recycling and remediating heavy metal ion caused by environmental pollution.Wax-like bud of the invention
Spore bacillus DIF1 has preferable electricity generation ability, can be used for microbiological fuel cell.
Purpose to realize the present invention, the invention adopts the following technical scheme:
In a first aspect, being preserved in China typical culture collection the present invention provides a kind of Bacillus cercus DIF1
The heart, preservation time are on May 15th, 2018, deposit number CCTCCNO:M2018274.
Bacillus cercus DIF1 of the present invention, is gram-positive bacteria, and bacterium is in the shape of a rod, end side, bacterium colony
In canescence circle, optimum growth temperature is 30 DEG C, and the most suitable growth pH value is 7.
Second aspect, the present invention provides application of the Bacillus cercus DIF1 in terms of dissimilatory reduction Fe (III).
The third aspect, the present invention provides application of the Bacillus cercus DIF1 in terms of hexavalent chrome reduction.
Fourth aspect, the present invention provides the microbial bacterial agent of Bacillus cercus DIF1 production, the microbial bacterial agents
It is fermented and is generated by Bacillus cercus DIF1.
Above-mentioned fermentation medium is LB liquid medium, and the LB liquid medium includes NaCl 8-12g/L, preferably
10g/L, yeast extract 4-6g/L, preferably 5g/L, peptone 8-12g/L, preferably 10g/L, pH 6.8-7.2, preferably
PH is 7.2.
5th aspect, the present invention provides the cultural method of Bacillus cercus DIF1, the method includes by wax-like bud
Spore bacillus DIF1 is inoculated in shaken cultivation in LB liquid medium.
The temperature of the shaken cultivation be 28-32 DEG C, preferably 30 DEG C, revolving speed 100-300rmp, preferably 120rpm.
6th aspect, the present invention provides microbial bacterias prepared by Bacillus cercus DIF1 or Bacillus cercus DIF1
Application of the agent in microbiological fuel cell.
The utility model has the advantages that compared with prior art, the isolated Bacillus cercus DIF1 of the present invention, which has stronger
The restoring function of dissimilatory reduction Fe (III)；Bacillus cercus DIF1 can also effectively restore Cr (VI) ion；Wax-like gemma bar
Bacterium DIF1 can be applied in microbiological fuel cell as electricity production bacterium；The present invention has widened people to Bacillus cercus
The application study thinking of (Bacillus cereus) in terms of its function is improvement of the dissimilatory iron reduction bacterium in environmental contaminants
Aspect provides new material, has stronger practical application value.
Detailed description of the invention
Fig. 1 is the bacterium colony electron microscopic picture of Bacillus cercus DIF1；
Fig. 2 is the 16SrDNA electrophoretogram of Bacillus cercus DIF1；
Fig. 3 is the bacterium colony figure of Bacillus cercus DIF1；
Fig. 4 is the phylogenetic tree of the Bacillus cercus DIF1 based on 16SrDNA sequence；
Fig. 5 is Bacillus cercus DIF1 iron reduction rate curve graph in the experiment of reduction ironic citrate；
Fig. 6 is Bacillus cercus DIF1 iron reduction rate curve graph in the experiment of reducing iron oxides；
Fig. 7 is curve graph of the Bacillus cercus DIF1 in reduction Cr (VI)；
Fig. 8 is temporal power curve of the Bacillus cercus DIF1 in microbiological fuel cell.
Specific embodiment
Technical solution of the present invention is further illustrated below with reference to embodiment and attached drawing.
Embodiment 1: separation, screening and the identification of bacterial strain
1.1 experimental article
Laboratory sample is derived from meishan iron mine Underground iron mine soil.
DM culture medium: NaHCO3 2.5g/L、CaCl2 0.08g/L、NH4Cl 1.0g/L、MgCl2·6H2O 0.2g/L、
NaCl 10g/L, HEPES 7.2g/L, yeast extract 0.5g/L, lactic acid 0.9g/L, Wolfe's vitamin solution (Wolfe's
Vitamin solution) 1mL/L, Wolfe's mineral solution (Wolfe's mineral solution) 10mL/L, pH
7.2, Wolfe's vitamin solutions and Wolfe's mineral solution pass through sterilizing filter membrane and the DM solution after high-temperature sterilization are added.
LB liquid medium: NaCl 10g/L, yeast extract 5g/L, peptone 10g/L, pH 7.2.
LB solid medium: NaCl 10g/L, yeast extract 5g/L, peptone 10g/L, agar 15g/L, pH7.2.
Cell cathode room electrolyte: potassium ferricyanide 50mmol/L, potassium dihydrogen phosphate 40mmol/L, dipotassium hydrogen phosphate
60mmol/L。
1.2 double-chamber microbiological fuel cells (MFC) assembling and starting
Bipolar chamber microbiological fuel cell is set up according to the following steps:
(1) determine what DM culture medium, the carbon plate electrode handled well and proton exchange membrane, newspaper that configuration finishes closely were wrapped
Bipolar chamber vial and rubber stopper processed are put into high-pressure sterilizing pot sterilizing, 121 DEG C of sterilizing 30min；
(2) experimental material that sterilizing finishes is put into superclean bench ultraviolet-sterilization 20min；
(3) two electrode chambers are connected with silicon rubber and proton exchange membrane, iron clamp, which is clamped, to be prevented from falling off；
(4) cathode chamber pours into 50mmol/L potassium ferricyanide solution 100mL, and filling 20g fine and closely woven iron ore soil in anode chamber simultaneously pours into
The DM culture solution of 100mL；
(5) anode chamber is packed into electrode, is sealed anode chamber with rubber stopper and sealed membrane, cathode chamber stays an aperture so as to oxygen
Gas is passed through, and battery is wrapped up with tinfoil；
(6) bipolar chamber that assembling finishes is put into constant incubator, and temperature is set as 25 DEG C.The external 5k Ω resistance of battery, is adopted
With the output voltage of the 30 channel usb data capture cards acquisition battery of Beijing Rui Bohua company.DM culture medium is regularly replaced weekly
And potassium ferricyanide solution, make battery stable operation, time interval is a cycle of operation.
The separation screening of 1.3 bacterial strains
On aseptic operating platform, take out electrode from the battery successfully started up, with choose bacteria stick from bacterium intensively from picking it is thin
Bacterium is coated with scribing line on configured solid LB media, is put into 30 DEG C of constant incubators, is incubated overnight.Choose the training of LB solid
The bacterium colony that feeding primary surface is individually grown is crossed on new LB solid medium, is incubated overnight.It is so repeated as many times, until thin
Bacterium growthform is stablized, as shown in Figure 3.The bacterial strain that purifying obtains is identified and (seen below), is Bacillus cercus, in
On May 15th, 2018 is preserved in China typical culture collection center, depositary institution address: Wuhan, China Wuhan University, preservation
Number be CCTCCNO:M2018274.
Stable culture dish is placed in 4 DEG C of refrigerators to save backup, meanwhile, with LB liquid medium shake bacterium (30 DEG C,
Bacterium solution 120rpm) obtained is mixed with 60% sterile glycerol 1:1, is put into minus 80 DEG C of refrigerators and is frozen.
1.4 strain characteristics and identification
(1) colonial morphology of bacterial strain are as follows: grown in LB solid medium, bacterium colony is in canescence, circle, Fig. 3 is seen, with leather
Blue Albert'stain Albert shows that bacterium is in the shape of a rod, and Fig. 1 sees in end side, is gram-positive bacteria, optimum growth temperature is 30 DEG C, most suitable
Growing pH value is 7.
(2) identification of 16SrDNA gene molecule is carried out to bacterial strain: using the core DNA of bacterium as template, with 16S rDNA gene
The universal primer of PCR amplification is primer, carries out PCR amplification, and obtaining length is the amplified band of 1418bp (with 1% Ago-Gel
Electrophoresis detection), as shown in Fig. 2, measuring its complete sequence after PCR product is purified.As a result as shown in SEQID No:1,16SrDNA
Gene order length is 1418bp, and 16SrDNA gene order is compared with GenBank database using BLAST analytic approach
Analysis, it is found that the affiliation of the bacterial strain and Bacillus cereus is closest, homology is up to 99% or more, is named as wax
Shape bacillus (Bacillus cereus) DIF1, the Bacillus cercus (Bacillus based on 16SrDNA gene order
Cereus) the phylogenetic evolution tree of DIF1 is as shown in Figure 4.
Embodiment 2: application of Bacillus cercus (Bacillus cereus) DIF1 in terms of restoring Fe (III)
2.1 Bacillus cercus (Bacillus cereus) DIF1 restores ironic citrate experiment:
DM solution is configured, 2mmol/L ironic citrate is added in solution, sterile 100ml serum bottle is added after high-temperature sterilization
In, according to the inoculum density of 2.1 parts, it is put into bacterium solution sealing, inoculum density 107/ L, control experiment are added without wax-like gemma
Bacillus (Bacillus cereus) DIF1 bacterium solution, is placed in dark culture in 30 DEG C of constant incubators.Every 12h, in aseptic operating platform
Sample of middle extraction utilizes the Fe of ultraviolet specrophotometer difference test sample according to the method for surveying iron reduction standard curve2+
Concentration variation and growth curve of bacteria (OD600), are as a result shown in Fig. 5, the results showed that Bacillus cercus (Bacillus cereus)
DIF1 is about 0.55mmol/L in 120h iron reducing power, and control experiment only has micro Fe2+It generates.
The experiment of 2.2 Bacillus cercus (Bacillus cereus) DIF1 reducing iron oxides:
DM solution is configured, 100mg/100ml iron oxide is added in solution.Sterile 100ml serum is added after high-temperature sterilization
In bottle, according to the inoculum density of 2.1 parts, it is put into bacterium solution sealing, control experiment is added without Bacillus cercus (Bacillus
Cereus) DIF1 bacterium solution is placed in half-light culture in 30 DEG C of constant incubators.Every 12h, a sample is extracted in aseptic operating platform
Product utilize the Fe of ultraviolet specrophotometer difference test sample2+Concentration variation and growth curve of bacteria (OD600), are as a result shown in figure
6, the results showed that Bacillus cercus (Bacillus cereus) DIF1 is about 0.62mmol/L in 96h iron reducing power, and right
According to the facts test only micro Fe2+It generates, illustrates that Bacillus cercus (Bacilluscereus) DIF1 has dissimilatory iron reduction ability.
It can be seen that from above experimental result, Bacillus cercus of the invention efficiently restores ironic citrate and iron oxide
In Fe (III), show Bacillus cercus of the invention (Bacillus cereus) DIF1 have stronger dissimilatory reduction Fe
(III) restoring function.
Embodiment 3: application of Bacillus cercus (Bacillus cereus) DIF1 in terms of hexavalent chrome reduction
Bacillus cercus (Bacillus cereus) DIF1 is to Cr (VI) reducing power confirmatory experiment:
DM solution is configured, 2mmol/L anhydrous sodium chromate is added in solution, sterile 100ml serum is added after high-temperature sterilization
In bottle, according to the inoculum concentration of 2.1 parts, it is put into the sealing of Bacillus cercus (Bacillus cereus) DIF1 bacterium solution, to according to the facts
It tests and is not put into bacterium solution, be placed in dark culture in 30 DEG C of constant incubators.Every 12h, a sample is extracted in aseptic operating platform, is pressed
According to the method for surveying chromium reduction standard curve, the Cr of ultraviolet specrophotometer difference test sample is utilized6+Concentration variation and bacterium are raw
Long curve (OD600), as a result as shown in Figure 7 display, 0~for 24 hours between, Cr6+Concentration reduces rapidly, Bacillus cercus
(Bacillus cereus) DIF1 is about 1.44mmol/L, control experiment Cr in 96h chromium reducing power6+Concentration is almost without change
Change, illustrates that Bacillus cercus (Bacillus cereus) DIF1 has preferable chromium reducing power.
Embodiment 4: application of Bacillus cercus (Bacillus cereus) DIF1 in microbiological fuel cell
Bacillus cercus (Bacillus cereus) DIF1 is inoculated with according to the concentration of 2.1 parts, is placed in the present invention
The double-chamber microbiological fuel cell anode of 1.2 parts building, measures power density curve, the output power of microbiological fuel cell
Density is the ratio between the device output power and electrode area of use, is the important finger for measuring microbiological fuel cell efficiency of fuel cell generation
Mark.This experiment acquires the closed circuit voltage of each MFC device using 30 channel usb data capture cards of Beijing Rui Bohua company, every
1h records the closed circuit voltage of primary each cell apparatus.The meter of output power density is obtained by Ohm's law and associated electrical formula
Calculate formula:
Wherein P closure indicates output power density, and E indicates the output voltage of cell apparatus, the external expression cell apparatus of R
Outer connecting resistance, S electrode indicate the area of carbon plate electrode.Known outer connecting resistance is 5k Ω, electrode area 4cm2.Gained power is close
Line write music as shown in figure 8, illustrate that Bacillus cercus (Bacillus cereus) DIF1 has preferable electricity generation ability, it can be with
For microbiological fuel cell.
As can be seen from the above results, the present invention isolated Bacillus cercus from meishan iron mine
(Bacilluscereus) DIF1, and find its stronger Fe (III) and Cr (VI) ion reduction function, and it is of the invention wax-like
Bacillus DIF1 is alternatively arranged as electricity production bacterium and is applied to microorganism battery, this has widened people to Bacillus cercus
(Bacilluscereus) application study thinking of the DIF1 in terms of its function, and be improvement of the iron-reducing bacterium in environmental contaminants
Aspect provides new material, has stronger practical application value.
Sequence table
<110>Southeast China University
Microbial bacterial agent and the application of<120>one plants of Bacillus cercus DIF1 and its production
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1418
<212> DNA
<213>Bacillus cercus DIF1 (Bacillus cereus DIF1)
<400> 1
gcagtcgagc gaatggatta agaagcttgc tcttatgaag ttagcggcgg acgggtgagt 60
aacacgtggg taacctgccc ataagactgg gataactccg ggaaaccggg gctaataccg 120
gataacattt tgaaccgcat ggttcgaaat tgaaaggcgg cttcggctgt cacttatgga 180
tggacccgcg tcgcattagc tagttggtga ggtaacggct caccaaggca acgatgcgta 240
gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga ctcctacggg 300
aggcagcagt agggaatctt ccgcaatgga cgaaagtctg acggagcaac gccgcgtgag 360
tgatgaaggc tttcgggtcg taaaactctg ttgttaggga agaacaagtg ctagttgaat 420
aagctggcac cttgacggta cctaaccaga aagccacggc taactacgtg ccagcagccg 480
cggtaatacg taggtggcaa gcgttatccg gaattattgg gcgtaaagcg cgcgcaggtg 540
gtttcttaag tctgatgtga aagcccacgg ctcaaccgtg gagggtcatt ggaaactggg 600
agacttgagt gcagaagagg aaagtggaat tccatgtgta gcggtgaaat gcgtagagat 660
atggaggaac accagtggcg aaggcgactt tctggtctgt aactgacact gaggcgcgaa 720
agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780
aagtgttaga gggtttccgc cctttagtgc tgaagttaac gcattaagca ctccgcctgg 840
ggagtacggc cgcaaggctg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 900
gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctctgaaa 960
accctagaga tagggcttct ccttcgggag cagagtgaca ggtggtgcat ggttgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gatcttagtt 1080
gccatcatta agttgggcac tctaaggtga ctgccggtga caaaccggag gaaggtgggg 1140
atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggacggt 1200
acaaagagct gcaagaccgc gaggtggagc taatctcata aaaccgttct cagttcggat 1260
tgtaggctgc aactcgccta catgaagctg gaatcgctag taatcgcgga tcagcatgcc 1320
gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag gagtttgtaa 1380
cacccggaaa gtcggtgggg taaacctttt ggagccag 1418
Claims (7)
1. one plant of Bacillus cercus DIF1 is preserved in China typical culture collection center, the preservation time is May 15 in 2018
Day, deposit number CCTCCNO:M2018274.
2. Bacillus cercus DIF1 according to claim 1, which is characterized in that it is gram-positive bacteria, and bacterium is in
Rod-shaped, end side, bacterium colony is in canescence circle.
3. application of the Bacillus cercus DIF1 according to claim 1 or 2 in terms of dissimilatory reduction Fe (III).
4. application of the Bacillus cercus DIF1 according to claim 1 or 2 in terms of hexavalent chrome reduction.
5. a kind of microbial bacterial agent produced using Bacillus cercus DIF1 described in as claimed in claim 1 or 22, which is characterized in that
The microbial bacterial agent is fermented by Bacillus cercus DIF1 to be generated.
6. the cultural method of Bacillus cercus DIF1 as claimed in claim 1 or 2, which is characterized in that the method includes
Bacillus cercus DIF1 is inoculated in shaken cultivation in LB liquid medium.
7. Bacillus cercus DIF1 according to claim 1 or 2 or microbial bacterial agent as claimed in claim 5 are micro-
Application in biological fuel cell.
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| CN110951642A (en) * | 2019-12-18 | 2020-04-03 | 东南大学 | Method for reducing and adsorbing heavy metal chromium by fixed microorganisms |
| CN114164139A (en) * | 2021-10-27 | 2022-03-11 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110669706A (en) * | 2019-11-26 | 2020-01-10 | 桂林理工大学 | Leersia hexandra endophytic bacterium capable of reducing hexavalent chromium as well as preparation method and application thereof |
| CN110669706B (en) * | 2019-11-26 | 2022-12-27 | 桂林理工大学 | Leersia hexandra endophytic bacterium capable of reducing hexavalent chromium and preparation method and application thereof |
| CN110951642A (en) * | 2019-12-18 | 2020-04-03 | 东南大学 | Method for reducing and adsorbing heavy metal chromium by fixed microorganisms |
| CN110951642B (en) * | 2019-12-18 | 2022-09-09 | 东南大学 | Method for reducing and adsorbing heavy metal chromium by fixed microorganisms |
| CN114164139A (en) * | 2021-10-27 | 2022-03-11 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
| CN114164139B (en) * | 2021-10-27 | 2023-03-10 | 福州大学 | Alkali-resistant chromium-resistant bacillus cereus and application thereof |
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