CN109503413A - A kind of monocycle ring flores aurantii alkane type sesquiterpene derivative and its preparation and application - Google Patents
A kind of monocycle ring flores aurantii alkane type sesquiterpene derivative and its preparation and application Download PDFInfo
- Publication number
- CN109503413A CN109503413A CN201910007383.8A CN201910007383A CN109503413A CN 109503413 A CN109503413 A CN 109503413A CN 201910007383 A CN201910007383 A CN 201910007383A CN 109503413 A CN109503413 A CN 109503413A
- Authority
- CN
- China
- Prior art keywords
- alkane type
- flores aurantii
- type sesquiterpene
- sesquiterpene derivative
- monocycle ring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
- C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C235/28—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and unsaturated
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/07—Optical isomers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/06—Systems containing only non-condensed rings with a five-membered ring
- C07C2601/08—Systems containing only non-condensed rings with a five-membered ring the ring being saturated
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The present invention relates to algae-inhibiting agent fields, specifically the monocycle ring flores aurantii alkane type sesquiterpene derivative in a kind of seaweed endogenetic fungus source and preparation method thereof and the application in terms of algal control.Shown in the structure such as formula (I) of monocycle ring flores aurantii alkane type sesquiterpene derivative, preparation method is that seaweed endogenetic fungus trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 is inoculated in fermented and cultured in fungi culture medium, tunning is after isolating and purifying, as monocycle ring flores aurantii alkane type sesquiterpene derivative shown in formula (I).The monocycle ring flores aurantii alkane type sesquiterpene derivative that the present invention obtains obtains compound to the half-inhibitory concentration of microalgae up to 2.6 mcg/mls through suppression activity of microalgae experiment.
Description
Technical field
The present invention relates to algae-inhibiting agent fields, the specifically a kind of monocycle ring flores aurantii alkane type times in seaweed endogenetic fungus source
Half triterpene derivative and preparation method thereof and the application in terms of algal control.
Background technique
In world wide, coastal waters Disaster And Prevention Measures of Red Tides increasingly sharpens, and seriously hinders sea fishery, offshore tourism and marine economy
Development.China enters the red tide high-incidence season after 2000, and red tide occurrence frequency and the extent of injury obviously increase.The main danger of red tide
There are four types of evil forms, first is that destroying marine ecology balance；Second is that destroying marine fishery resources；Third is that endangering human health；Fourth is that
Influence the development of marine tourism.According to statistics, the economic loss as caused by red tide is up to 1,000,000,000 yuan or more every year.It can draw in ocean
The microalgae of red tide is sent out there are about 300 kinds, wherein about 80 kinds of toxic red tide algae.Scientificlly and effectively prevent and administer red tide to be the world today
One of property problem.
Currently, the Preventing Countermeasures of red tide other than preventing seawater eutrophication, will also contain the i.e. red tide algae of red tide plankton
Development, and contain that the method for red tide algae development is mainly that chemical agent is administered.Although chemical agent such as copper sulphate, chlorine etc. is fast
Speed effectively kills or inhibits harmful algae or inhibits its growth and breeding, but in the presence of the harm to non-red tide plankton, medicament residue
And the secondary pollution problems to environment.
Compared with administering traditional chemical agent used in red tide, marine organisms source natural drug have it is with strong points,
Highly-safe, active significant and environmentally friendly advantage, to solve the problems, such as that the prevention and treatment of the red tide caused at present by red tide algae mentions
New thinking is supplied
Summary of the invention
The object of the present invention is to provide a kind of monocycle ring flores aurantii alkane type sesquiterpene derivative and its preparations and application.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of monocycle ring flores aurantii alkane type sesquiterpene derivative, the structure such as formula of monocycle ring flores aurantii alkane type sesquiterpene derivative
(I) shown in
A kind of preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative, by trichoderma asperellum (Trichoderma
Asperellum) A-YMD-9-2 is inoculated in fermented and cultured in fungi culture medium, after tunning is purified, as shown in formula (I)
Monocycle ring flores aurantii alkane type sesquiterpene derivative；Described trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2
China typical culture collection center CCTCC is stored on June 27th, 2018, deposit number is CCTCC M 2018405.
Specifically:
1) trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 is inoculated in fungi culture medium and is fermented
10-60 days, and after extract and be concentrated through organic solvent, as crude extract；
2) it takes the crude extract in step 1) to carry out silica gel column chromatography, carries out gradient elution through eluent, collect elution fraction,
Elution fraction is detected through thin-layer chromatography；
3) collection step 2) in elution fraction again successively inverted silica gel column chromatography and half preparative high-performance liquid chromatographic separation
Purifying is to get the monocycle ring flores aurantii alkane type sesquiterpene derivative as shown in formula (I).
Fungi culture medium is rice solid medium, jerusalem artichoke dextrose broth or potato glucose in step 1)
Fluid nutrient medium.
Organic solvent is ethyl acetate, petroleum ether, n-hexane, hexamethylene, methylene chloride, methanol, ethyl alcohol, propyl alcohol or isopropyl
It is one or more of in alcohol.
Petroleum ether-ethyl acetate, the petroleum ether-ethyl alcohol, petroleum ether-that organic solvent in step 2) is volume ratio 50-0:1
Propyl alcohol, petroleum ether-isopropanol, dichloromethane-ethyl acetate, methylene chloride-methanol, dichloromethane-ethanol, methylene chloride-the third
One group in alcohol, methylene chloride-isopropanol or several groups.
Step 3) the reversed-phase silica gel column chromatography eluent is the water-methanol or water-ethanol of volume ratio 5-0:1；Half prepares
High performance liquid chromatography eluent gradient is the water-methanol or water-acetonitrile of volume ratio 10-0:1.
A kind of application of monocycle ring flores aurantii alkane type sesquiterpene derivative, the monocycle ring flores aurantii alkane type sesquiterpene derivative
It is used to prepare the algae-inhibiting agent of microalgae.
The microalgae is the Phaeocystis globosa for causing red tide to endanger.
The invention has the following advantages that the present invention is by being located away from the trichoderma asperellum (Trichoderma of marine red alga fragrant plant mentioned in ancient texts
Asperellum) A-YMD-9-2 ferment it is extracted, separation obtain monocycle ring flores aurantii alkane type sesquiterpene derivative, it is inhibited micro-
Algae activity experiment show that compound is 2.6 mcg/mls to the half-inhibitory concentration for the Phaeocystis globosa that can cause red tide.
Specific embodiment:
The present invention is further elaborated below with reference to embodiment.
Embodiment 1
Shown in the structure such as formula (I) of the monocycle ring flores aurantii alkane type sesquiterpene derivative in seaweed endogenetic fungus source.
The compound has following physical and chemical and spectral characteristic:
Colorless oil；Specific rotatory power [α]20 D-19(c 0.63,MeOH)；Nuclear magnetic resonance spectroscopy (solvent is deuterated chloroform) δH
1.01d(6.8),1.54m,1.66m,1.52m,1.82m,1.51m,1.82m,1.45m,1.63m,4.28td(7.9,6.3),
4.90s,4.84s,1.23s,1.11s,1.73s,5.67br s,2.31t(6.0),3.73t(6.0),2.11d(0.7),
6.12br d(8.2),；Carbon-13 nmr spectra (solvent is deuterated chloroform) δC 14.6CH3,44.5CH,81.4C,40.4CH2,
24.5CH2,54.9CH,74.8C,36.4CH2,27.6CH2,54.8CH,145.1C,111.7CH2,26.2CH3,24.9CH3,
19.5CH3,166.9C,120.7CH,149.9C,43.7CH2,60.0CH2,18.3CH3,；High resolution mass spectrum [M]+m/
Z367.2731, calculated value 367.2723.
Embodiment 2
The preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative as shown in formula (I):
It makes even well-grown trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 strain on plate, is cut into
Fritter is simultaneously inoculated in rice solid medium, puts 50 grams of rice solid mediums in every 1 liter of triangular flask, totally 200 bottles, room temperature is quiet
It only ferments 40 days, is then extracted with ethyl acetate three times, is concentrated under reduced pressure, 212.4 grams of crude extract are obtained after concentration.
The rice solid medium group become every liter contain 500 grams of rice, 6 grams of peptone, 500 milliliters of distilled water, Chen Hai
500 milliliters of water.
During trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 strain was stored on June 27th, 2018
State Type Tissue Collection CCTCC, address: Wuhan, China university, deposit number are CCTCC M 2018405, classification life
Entitled Trichoderma asperellum, strain number are A-YMD-9-2.
Silica gel column chromatography by crude extract through 100-200 mesh successively uses volume ratio 50:1,20:1,10:1,5:1,2:1,1:
1 arrives the methylene chloride-methanol progress gradient elution of the petroleum ether-ethyl acetate of 0:1 and 10:1,5:1,2:1,1:1 to 0:1, point
Not Shou Ji eluent, each component of collection use again thin-layer chromatography (TLC) detect (the methylene chloride-methanol exhibition of volume ratio 50-1:1
Open, anisaldehyde-sulfuric acid colour developing or the ultraviolet light detection of 254nm), judged according to Rf value, merge same or like part, obtains 8
A component (1-8).
It is the component 7 of 0.6-0.7 (methylene chloride-methanol of volume ratio 6:1 is unfolded, the ultraviolet light detection of 254nm) by Rf value,
I.e. with the successively inverted C again of the component under the methylene chloride-methanol gradient elution of volume ratio 5:118Silicagel column and half is prepared efficiently
Liquid chromatogram separation.Reverse phase C18Silica gel column chromatography eluent is the water-methanol of volume ratio 1:1, and TLC detects (the two of volume ratio 6:1
Chloromethanes-methanol expansion, the ultraviolet light detection of 254nm, anisaldehyde-sulfuric acid colour developing), collecting under 254nm ultraviolet light has fluorescence, fennel
Aldehyde-sulfuric acid shows red component；Component is collected again through half preparative high-performance liquid chromatographic, and gradient is volume ratio 4-1.5:1's
Water-acetonitrile, elution time are 30 minutes, collect the component of 21 minutes appearances, as compound shown in formula (I) (14.7 milligrams).
Through thin-layer chromatography detection (expansion of volume ratio 6:1 methylene chloride-methanol, the ultraviolet light detection of 254nm, anisaldehyde-sulfuric acid colour developing), it is in
Individually, uniformly fluorescence, aobvious punctation, are determined as pure compound.Through Spectrum Analysis, Structural Identification is a kind of monocycle ring flores aurantii
Alkane type sesquiterpene derivative, structural formula is such as shown in (I).
Embodiment 3
Difference from Example 2 is
It makes even well-grown trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 strain on plate, is cut into
Fritter is simultaneously inoculated in jerusalem artichoke dextrose broth, puts 300 milliliters of culture mediums in every 1 liter of triangular flask, and totally 200 bottles, room temperature
Static fermentation 40 days is then extracted three times with methylene chloride, is concentrated under reduced pressure, 200 grams of crude extract are obtained after concentration.
The jerusalem artichoke dextrose broth group becomes every liter of 500 milliliters of liquor for containing 200 grams of jerusalem artichoke stem tubers, glucose
20 grams, 5 grams of peptone, 500 milliliters of Chen Haishui.
Silica gel column chromatography by crude extract through 200-300 mesh, successively with volume ratio 50:1,30:1,15:1,10:1,5:1,
Petroleum ether-ethyl alcohol progress gradient elution of 2:1,1:1 to 0:1, collect eluent, then detect (volume ratio with thin-layer chromatography respectively
The petroleum ether of 50-0:1-ethyl alcohol expansion, anisaldehyde-sulfuric acid colour developing or the ultraviolet light detection of 254nm), judged according to Rf value, merged
Same or like part obtains 8 components (1-8).
It is 0.6-0.7 (petroleum ether of volume ratio 2:1-ethyl alcohol expansion, the ultraviolet light detection of 254nm, anisaldehyde-sulphur by Rf value
Acid colour developing) component 7, i.e., with the successively inverted C again of the component under petroleum ether-ethanol gradient elution of volume ratio 2:118Silicagel column
It is separated with half preparative high-performance liquid chromatographic.Reverse phase C18Silica gel column chromatography eluent is the water-ethanol of volume ratio 1:1, TLC detection
(petroleum ether of volume ratio 2:1-ethyl alcohol expansion, the ultraviolet light detection of 254nm, anisaldehyde-sulfuric acid colour developing), collects 254nm ultraviolet light
Under there is fluorescence and anisaldehyde-sulfuric acid to show red component；Component is collected again through half preparative high-performance liquid chromatographic, gradient is body
Water-methanol of the product than 4-1:1, elution time are 30 minutes, collect the component of 21 minutes appearances, obtain the orange of monocycle ring shown in formula (I)
Flower alkane type sesquiterpene derivative.
Embodiment 4
Press down activity of microalgae experiment:
Specifically: activation is diluted to certain for trying microalgae, the microalgae of logarithmic growth phase with sterilized f/2 culture medium
Frustule concentration (about 5 × 104A/milliliter), 96 orifice plates are taken, every hole adds 195 microlitres of algae solutions to be test cultures plate.Sample sets and
Control group respectively sets three in parallel, and sample sets add 5 microlitres of concentration to be the compound solution of 4 mg/mls, and solvent is dimethyl sulfoxide
(DMSO), blank control group adds 5 microlitres of solvents, and positive controls add 5 microlitres of concentration to be the K of 4 mg/mls2Cr2O7Solution.Training
Under the conditions of supporting 20 DEG C, intensity of illumination 2000Lux, Light To Dark Ratio 14:10 of temperature (hour), cultivate 24 hours.Wherein it is for examination microalgae
Phaeocystis globosa.
Using blood counting chamber, microalgae in each experimental group is counted under the microscope.To inhibiting rate greater than 50%
Experimental group further calculates its half-inhibitory concentration IC50Value.By the compound solution of configured final concentration of 4 mg/ml
Successively be diluted, finally obtain component that 15 groups of concentration successively reduce (1000,500,200,100,50,20,10,5,2,1,
0.5,0.25,0.125,0.0625,0.03125 mcg/ml).Observe and calculate the microalgae suppression of compound under each concentration gradient
Rate processed takes inhibiting rate at least five concentration gradient between 0-100, calculates IC50Value.
Experimental result: the monocycle ring flores aurantii alkane type sesquiterpene derivative of above-mentioned acquisition inhibits dense to the half of Phaeocystis globosa
Degree is 2.6 mcg/mls, has the function of inhibiting Phaeocystis globosa.
Claims (8)
1. a kind of monocycle ring flores aurantii alkane type sesquiterpene derivative, it is characterised in that: monocycle ring flores aurantii alkane type sesquiterpene derivative
Shown in structure such as formula (I)
2. a kind of preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative described in claim 1, it is characterised in that: by spine
Spore trichoderma (Trichoderma asperellum) A-YMD-9-2 is inoculated in fermented and cultured in fungi culture medium, tunning warp
After purification, as monocycle ring flores aurantii alkane type sesquiterpene derivative shown in formula (I)；Trichoderma asperellum (the Trichoderma
Asperellum) A-YMD-9-2 is stored in China typical culture collection center CCTCC, deposit number on June 27th, 2018
For CCTCC M 2018405.
3. the preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative as described in claim 2, it is characterised in that specific system
Standby step:
1) trichoderma asperellum (Trichoderma asperellum) A-YMD-9-2 is inoculated in fungi culture medium the 10-60 that ferments
It, and after extract and be concentrated through organic solvent, as crude extract；
2) it takes the crude extract in step 1) to carry out silica gel column chromatography, carries out gradient elution through eluent, collect elution fraction, elution
Group lease making thin-layer chromatography detection；
3) collection step 2) in elution fraction successively inverted silica gel column chromatography and half preparative high-performance liquid chromatographic isolate and purify again,
Up to the monocycle ring flores aurantii alkane type sesquiterpene derivative as shown in formula (I).
4. the preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative according to claim 3, it is characterised in that: step 1)
Middle fungi culture medium is rice solid medium, jerusalem artichoke dextrose broth or potato dextrose broth；
Organic solvent is in ethyl acetate, petroleum ether, n-hexane, hexamethylene, methylene chloride, methanol, ethyl alcohol, propyl alcohol or isopropanol
It is one or more of.
5. the preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative according to claim 3, it is characterised in that: step 2)
In organic solvent be volume ratio 50-0:1 petroleum ether-ethyl acetate, petroleum ether-ethyl alcohol, petroleum ether-propyl alcohol, petroleum ether-it is different
Propyl alcohol, dichloromethane-ethyl acetate, methylene chloride-methanol, dichloromethane-ethanol, methylene chloride-propyl alcohol, methylene chloride-are different
One group in propyl alcohol or several groups.
6. the preparation method of monocycle ring flores aurantii alkane type sesquiterpene derivative according to claim 3, it is characterised in that: step 3)
The reversed-phase silica gel column chromatography eluent is the water-methanol or water-ethanol of volume ratio 5-0:1；Half preparative high-performance liquid chromatographic is washed
The water-methanol or water-acetonitrile that de- liquid gradient is volume ratio 10-0:1.
7. a kind of application of monocycle ring flores aurantii alkane type sesquiterpene derivative described in claim 1, it is characterised in that: the list
Ring ring flores aurantii alkane type sesquiterpene derivative is used to prepare the algae-inhibiting agent of microalgae.
8. the application of monocycle ring flores aurantii alkane type sesquiterpene derivative according to claim 7, it is characterised in that: the microalgae is
The Phaeocystis globosa for causing red tide to endanger.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910007383.8A CN109503413B (en) | 2019-01-04 | 2019-01-04 | Monocyclic ring nerolidine type sesquiterpene derivative and preparation and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910007383.8A CN109503413B (en) | 2019-01-04 | 2019-01-04 | Monocyclic ring nerolidine type sesquiterpene derivative and preparation and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109503413A true CN109503413A (en) | 2019-03-22 |
| CN109503413B CN109503413B (en) | 2021-09-28 |
Family
ID=65756258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910007383.8A Active CN109503413B (en) | 2019-01-04 | 2019-01-04 | Monocyclic ring nerolidine type sesquiterpene derivative and preparation and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109503413B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988180A (en) * | 2019-04-26 | 2019-07-09 | 中国科学院烟台海岸带研究所 | A kind of Diketopiperazine derivative and its preparation and application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225078A (en) * | 2008-01-18 | 2008-07-23 | 中国科学院海洋研究所 | Sea weed sesquiterpenoids as well as preparation method and application thereof |
| CN101787008A (en) * | 2009-01-23 | 2010-07-28 | 中国科学院上海药物研究所 | N-acetyl neuraminic acid compounds, medicine compositions thereof and preparation methods and purposes thereof |
| CN102659521A (en) * | 2012-04-09 | 2012-09-12 | 中国科学院烟台海岸带研究所 | Alga bromosesquiterpene compound, preparation thereof and application thereof |
| FR3012725A1 (en) * | 2013-11-07 | 2015-05-08 | Ard Sa | COMPOSITIONS OF BIOLOGICAL CONTROL AGENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF |
| CN107668374A (en) * | 2017-10-03 | 2018-02-09 | 长沙仲善新能源科技有限公司 | A kind of single cell protein biological feedstuff and preparation method thereof |
| CN108409539A (en) * | 2018-04-23 | 2018-08-17 | 江苏省海洋资源开发研究院(连云港) | A kind of preparation method and purposes of Porphyra yezoensis sesquiterpenoids algistatic activity compound |
| CN108467337A (en) * | 2018-04-23 | 2018-08-31 | 中国科学院烟台海岸带研究所 | A kind of sesquiterpene alcohols compound and its preparation and application |
-
2019
- 2019-01-04 CN CN201910007383.8A patent/CN109503413B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225078A (en) * | 2008-01-18 | 2008-07-23 | 中国科学院海洋研究所 | Sea weed sesquiterpenoids as well as preparation method and application thereof |
| CN101787008A (en) * | 2009-01-23 | 2010-07-28 | 中国科学院上海药物研究所 | N-acetyl neuraminic acid compounds, medicine compositions thereof and preparation methods and purposes thereof |
| CN102659521A (en) * | 2012-04-09 | 2012-09-12 | 中国科学院烟台海岸带研究所 | Alga bromosesquiterpene compound, preparation thereof and application thereof |
| FR3012725A1 (en) * | 2013-11-07 | 2015-05-08 | Ard Sa | COMPOSITIONS OF BIOLOGICAL CONTROL AGENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF |
| CN107668374A (en) * | 2017-10-03 | 2018-02-09 | 长沙仲善新能源科技有限公司 | A kind of single cell protein biological feedstuff and preparation method thereof |
| CN108409539A (en) * | 2018-04-23 | 2018-08-17 | 江苏省海洋资源开发研究院(连云港) | A kind of preparation method and purposes of Porphyra yezoensis sesquiterpenoids algistatic activity compound |
| CN108467337A (en) * | 2018-04-23 | 2018-08-31 | 中国科学院烟台海岸带研究所 | A kind of sesquiterpene alcohols compound and its preparation and application |
Non-Patent Citations (3)
| Title |
|---|
| KOSHINO HIROYUKI ET AL: "New fungitoxic sesquiterpenoids, chokols A-G, from stromata of Epichloe typhina and the absolute configuration of chokol E", 《AGRICULTURAL AND BIOLOGICAL CHEMISTRY》 * |
| YIN PING SONG ET AL: "Tricholumin A, a Highly Transformed Ergosterol Derivative from the Alga-Endophytic Fungus Trichoderma asperellum", 《ORGANIC LETTERS》 * |
| 梁小蕊等: "海藻内生真菌棘孢木霉dl-34中的二萜和甾体化合物", 《化学与生物工程》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988180A (en) * | 2019-04-26 | 2019-07-09 | 中国科学院烟台海岸带研究所 | A kind of Diketopiperazine derivative and its preparation and application |
| CN109988180B (en) * | 2019-04-26 | 2021-09-28 | 中国科学院烟台海岸带研究所 | Diketopiperazine derivative and preparation and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109503413B (en) | 2021-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108467337A (en) | A kind of sesquiterpene alcohols compound and its preparation and application | |
| CN109503414A (en) | One kind alkane type sesquiterpene derivative of flores aurantii containing azo-cycle and its preparation and application | |
| CN106434783A (en) | Benzopyrone compound, benzopyrone compound preparation method and application of benzopyrone compound to preparation of antibacterial medicines | |
| CN109336873B (en) | Compound lithocarolsA-F, preparation method thereof and application thereof in preparation of antitumor drugs | |
| CN102174051A (en) | Strong algae activity inhibiting compound as well as preparation method and application thereof | |
| CN108383707A (en) | A kind of daucane sesquiterpenoids and its preparation and application | |
| CN108484363A (en) | A kind of three alcohol compound of sequiterpene and its preparation and application | |
| CN109503535A (en) | A kind of bicyclic ring flores aurantii alkane type sesquiterpene derivative and its preparation and application | |
| Sun et al. | Sesquiterpenoids with antialgal activity against the common red tide microalgae from marine macroalga Porphyra yezoensis | |
| CN109503413A (en) | A kind of monocycle ring flores aurantii alkane type sesquiterpene derivative and its preparation and application | |
| CN108373457A (en) | A kind of epoxy sesquiterpenoids and its preparation and application | |
| CN110862371B (en) | Polycyclic polyketone compound, preparation method thereof and application thereof in preparation of antibacterial drugs | |
| CN109503428A (en) | A kind of ring flores aurantii alkane type hydroxamic acid derivative and its preparation and application | |
| CN109956883A (en) | A kind of acetylation flores aurantii containing azo-cycle alkane type sesquiterpene derivative and its preparation and application | |
| CN109988180A (en) | A kind of Diketopiperazine derivative and its preparation and application | |
| CN112111409A (en) | Penicillium oxalicum and application thereof | |
| CN109776561B (en) | Compounds Cytorhizins B and C, preparation method thereof and application thereof in preparation of antitumor drugs | |
| CN108467398A (en) | A kind of diketopiperazine compound and its preparation and application | |
| CN115536645A (en) | Compound Phonolide B, preparation method thereof and application thereof in antibacterial drugs | |
| CN109678749A (en) | A kind of ring flores aurantii alkane type sequiterpene amide and its preparation and application | |
| CN109627192A (en) | A kind of ring flores aurantii alkane type sesquiterpene derivative and its preparation and application | |
| CN110003041A (en) | A kind of ring flores aurantii alkane acylated derivatives and its preparation and application | |
| CN102786528A (en) | Polyoxybiotic alkali compound as well as preparation method and application thereof | |
| CN113621526A (en) | Marine fungus Aspergillus versicolor M-7-SW9, mixed source terpenoid and extraction method and application thereof | |
| CN108586218A (en) | A kind of chloro-cyclopentene ketone compounds and its preparation and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |