CN109497077A - Application of the Ke Buti rhzomorph B in preparation bacterium or fungistat - Google Patents
Application of the Ke Buti rhzomorph B in preparation bacterium or fungistat Download PDFInfo
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- CN109497077A CN109497077A CN201811421548.8A CN201811421548A CN109497077A CN 109497077 A CN109497077 A CN 109497077A CN 201811421548 A CN201811421548 A CN 201811421548A CN 109497077 A CN109497077 A CN 109497077A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses application of the Ke Buti rhzomorph B in preparation bacterium or fungistat, applicant has found Ke Buti rhzomorph B(Kobutimycin B for the first time) there is inhibiting effect to various bacteria and fungi, bacterium includes Solanaceae Ralstonia solanacearum, rice Xanthomonas, pseudomonas syringae, staphylococcus aureus, citrus Xanthomonas campestris；Fungi includes eggplant V. albo-altrum, Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, botrytis cinerea, gibberella saubinetii, rape endophytic bacterial, citrus Penicillium notatum.The substance especially has stronger antagonism to Ke Buti rhzomorph B, provides selectivity to prevent and treat the preparation of bacterial wilt biological agent.
Description
Technical field
The invention belongs to technical field of agricultural microbiology, and in particular to Ke Buti rhzomorph B is in preparation bacterium or fungi suppression
Application in preparation.
Background technique
Bacterial wilt (Bacterial wilt disease) is Ragnarok soil-borne disease, and it is big to occupy the world ten
Bacteriosis second because of its rapid onset, lacks cost-effective control measure, referred to as " cancer " of plant.Its cause of disease is
Ralstonia solanacearum (Ralstonia solanacearum, abbreviation Ralstonia solanacearum), the bacterium generally invade from plant root or wound,
The mass propagation in vascular bundle, leads to vascular bundle blocking, necrosis, and here dead plant hydropenia withers.According to statistics, which can infect 45
300 various plants of section, aggrieved the most serious with solanaceous crops, including tomato, eggplant, capsicum, tobacco, potato etc., grave illness field
The death rate is up to 90% or more, brings crushing loss to agricultural production.
The control method of Solanaceae bacterial wilt includes chemical prevention, cultural control, soil disinfection and biological control etc..(1) change
Learn prevention and treatment: also referred to as pesticide control, is the most common method of controlling plant diseases.Currently, prevention and treatment bacterial wilt is the most commonly used
Chemical agent is agricultural streptomycin, because of its residual, toxicity and the problems such as Environmental security, is completely forbidden by the Ministry of Agriculture and is used as agriculture
Medicine uses.(2) cultural control: using rice field-upland field rotation, crop rotation etc., changing cropping pattern or tillage method etc. avoid continuous cropping
With reduction soil bacteria containing amount.(3) soil disinfection: carrying out disinfection to soil using chemical agent, thoroughly kill soil surface characters,
In it is best with bromomethane Disinfection Effect, but because of its destruction to ozone layer, and whole world disabling.(4) biological control: biological control tool
There is the features such as environment compatibility is good, highly-safe, is Solanaceae bacterial wilt study on prevention emphasis.
Ke Buti rhzomorph B (Kobutimycin B) is a kind of microbe-derived antibiotic, and report is to bacillus subtilis at present
Bacterium (Bacillus subtilis), Escherichia coli (Escherichia coli), saccharomycete (Saccharomyces
) etc. cerevisiae there is antagonism.However, inhibiting effect of the Ke Buti rhzomorph B to Ralstonia solanacearum, there is no any report.
Summary of the invention
It is an object of the present invention to provide application of the Ke Buti rhzomorph B in preparation bacterium or fungistat, institutes
The bacterium stated includes Solanaceae Ralstonia solanacearum, rice Xanthomonas, pseudomonas syringae, staphylococcus aureus, citrus Xanthomonas campestris；
The fungi includes eggplant V. albo-altrum, Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, botrytis cinerea, and wheat is red
Mould, rape endophytic bacterial, citrus Penicillium notatum；Ke Buti rhzomorph B especially has preferable Solanaceae Ralstonia solanacearum to Ke Buti rhzomorph B
Inhibitory activity provides selectivity to prevent and treat the preparation of bacterial wilt biological agent.
In order to reach the goals above, the present invention uses following technical measures:
Ke Buti rhzomorph B (5-Methyl-7- (2'- (2 "-methylbutyryloxy) -3'- of the present invention
Acetoxy) butylidene-1a, 2,3,7-tetrahydrocyclopent (b) oxireno (c) pyridine), molecular weight
It is 361.43, molecular formula is C20H27NO5, chemical formula is as follows:
Application of the Ke Buti rhzomorph B in preparation bacterium or fungistat, including using Ke Buti rhzomorph B as uniquely having
Effect ingredient is used to prepare bacterium or fungistat as one of effective component, and the bacterium includes Solanaceae Ralstonia solanacearum, water
Rice Xanthomonas campestris, pseudomonas syringae, staphylococcus aureus, citrus Xanthomonas campestris；The fungi includes eggplant V. albo-altrum,
Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, botrytis cinerea, gibberella saubinetii, rape endophytic bacterial, citrus mould
Bacterium.
Ke Buti rhzomorph B is treated or prevented in preparation by the application in the drug of bacterial disease or fungus-caused disease；Institute
The bacterial disease stated includes that rice Xanthomonas, pseudomonas syringae, staphylococcus aureus disease, citrus Xanthomonas campestris and Solanaceae are green
Withered bacterium；The fungi includes eggplant V. albo-altrum, Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, botrytis cinerea,
Gibberella saubinetii, rape endophytic bacterial, citrus Penicillium notatum.
Compared with prior art, the invention has the following advantages that
First: present invention firstly discovers that it is with anti-Solanaceae germ activity；
Second: the compound is strong to Solanaceae Ralstonia solanacearum antagonistic ability, and minimal inhibitory concentration only has 14.2 μ g/ml；It is new
The preparation of type Solanaceae Ralstonia solanacearum disease biological prevention and control agent provides new selectivity.
Detailed description of the invention
Fig. 1 is the antibacterial figure of the anti-Solanaceae Ralstonia solanacearum compound of Ke Buti rhzomorph B.
Specific embodiment
Experimental method in following embodiments is unless otherwise instructed the microbiology conventional practices of report.City
Purchase or synthesis all achievable present invention of Ke Buti rhzomorph B.Ke Buti rhzomorph B used in the embodiment of the present invention, by public
The usual manner synthesis opened, chemical formula are as follows:
Embodiment 1:
Cylinder-plate method measures the anti-Solanaceae Ralstonia solanacearum minimal inhibitory concentration of Ke Buti rhzomorph B
Principle: deriving according to diffusion law, the logarithm of antibiotic total amount and antibacterial radius it is square linear.When
When antibiotic concentration reaches at or above MIC value, test organisms is just suppressed and irreproducible, so that transparent inhibition zone be presented.
LogY=kX2+ b (Y is concentration, and X is inhibition zone radius)
1) Solanaceae Ralstonia solanacearum scribing line activation obtains single colonie, and picking single colonie is inoculated with 28 DEG C of SPA culture medium, 150rpm culture
To logarithmic growth phase.
2) compound concentration 7.1, the Ke Buti rhzomorph B of 14.2,28.4,56.8,113.6 μ g/mL are right using water as feminine gender
According to.
3) 1% pathogen is added to the solid medium (being added when shaking flask non-scald on hand) melted, is put in good plate
Set Oxford cup (internal diameter 6mm, outer diameter 7.8mm, high 10mm, the same below) and be added 100ul various concentration Ke Buti rhzomorph B it is molten
Result is observed in liquid, 28 DEG C of incubator cultures.
Using above-mentioned experiment, the equation of acquisition is LogY=0.0005X2+ 1.1739, measuring minimal inhibitory concentration is 14.2
μg/ml。
Experimental example 2:
Cylinder-plate method measures the anti-encountered pathogenic bacterial activity of Ke Buti rhzomorph B:
1) pathogen Xanthomonas oryzae pv oryzae (Xanthomonas oryzae), pseudomonas syringae (Pseudomonas
Syringa e), citrus Xanthomonas campestris (Xanthomonascitri) activation after, picking single colonie be inoculated with 28 DEG C of LB culture medium,
150rp m is cultivated to logarithmic growth phase.Pathogen staphylococcus aureus (Staphylococcus aureus) at 28 DEG C,
150rpm is cultivated to logarithmic growth phase.
2) the Ke Buti rhzomorph B of 100 μ g/mL of compound concentration, using water as negative control.
3) solid medium (1ml bacterium solution+99ml culture medium) melted is added in 1% pathogen (to add when shaking flask non-scald on hand
Enter), Oxford cup is placed in good plate and 100ul cloth is added mentions rhzomorph B, 3 repetitions, and staphylococcus aureus is placed in 37
DEG C culture, water Xanthomonas oryzae pv.oryzae, avenae subsp.citrull, citrus processing be placed in 28 DEG C of cultures, and the results are shown in Table 1.
1: Ke Buti rhzomorph B antibacterium of table spectrum
+: indicate effective ,-: it indicates to no effect
Experimental example 3:
Punch method measures the anti-encountered pathogenic fungi activity of Ke Buti rhzomorph B
1) disease fungus eggplant V. albo-altrum (Fusariumjsolani), Fusarium oxysporum (Fusarium oxysporum),
Verticillium dahliae (Verticillium dahliae), rice rice blast fungus (Magnaportheoryzae), botrytis cinerea pers
(Borrytisciner ea), fusarium graminearum (Fusarium graminearum), rape endophytic bacterial (sclerotinia sclerotiorum
Bacterium), Penicillium italicum bacterium (Penicillium italicum) activation, 28 DEG C of picking mycelium inoculation PDA culture medium cultures.
2) the Ke Buti rhzomorph B of 100 μ g/mL of compound concentration, using water as negative control.
3) picking fungus block is in PDA solid medium tablets center, is made a call to 6 holes with punch is equidistant (diameter in hole is
4mm), 20 μ l Ke Buti rhzomorph B are added, 3 repetitions are placed in 28 DEG C of cultures, and the results are shown in Table 2 for observation.
2: Ke Buti rhzomorph B anti-fungus spectra of table
+: indicate effective ,-: it indicates to no effect.
Claims (2)
1. application of the Ke Buti rhzomorph B in preparation bacterium or fungistat, the bacterium includes Solanaceae Ralstonia solanacearum, rice
Xanthomonas campestris, pseudomonas syringae, staphylococcus aureus, citrus Xanthomonas campestris；The fungi includes eggplant V. albo-altrum, point
Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, botrytis cinerea, gibberella saubinetii, rape endophytic bacterial, citrus Penicillium notatum.
2. Ke Buti rhzomorph B is treated or prevented in preparation by the application in the drug of bacterial disease or fungus-caused disease, described
Bacterial disease include rice Xanthomonas, pseudomonas syringae, staphylococcus aureus disease, citrus Xanthomonas campestris and Solanaceae blueness it is withered
Bacterium；The fungi includes eggplant V. albo-altrum, Fusarium oxysporum, verticillium dahliae, rice rice blast fungus, and botrytis cinerea is small
Wheat gibberella, rape endophytic bacterial, citrus Penicillium notatum.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811421548.8A CN109497077B (en) | 2018-11-26 | 2018-11-26 | Application of corybuterol B in preparation of bacterial or fungal inhibitor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811421548.8A CN109497077B (en) | 2018-11-26 | 2018-11-26 | Application of corybuterol B in preparation of bacterial or fungal inhibitor |
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| CN109497077A true CN109497077A (en) | 2019-03-22 |
| CN109497077B CN109497077B (en) | 2020-11-24 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05262776A (en) * | 1990-11-20 | 1993-10-12 | Hokko Chem Ind Co Ltd | New antibiotic ta3341-a and ta3341-b and their production |
| WO2015017866A1 (en) * | 2013-08-02 | 2015-02-05 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
-
2018
- 2018-11-26 CN CN201811421548.8A patent/CN109497077B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05262776A (en) * | 1990-11-20 | 1993-10-12 | Hokko Chem Ind Co Ltd | New antibiotic ta3341-a and ta3341-b and their production |
| WO2015017866A1 (en) * | 2013-08-02 | 2015-02-05 | Enevolv, Inc. | Processes and host cells for genome, pathway, and biomolecular engineering |
Non-Patent Citations (1)
| Title |
|---|
| KENJI KANBE等: ""KOBUTIMYCINS A AND B, NEW ALKALOID ANTIBIOTICS PRODUCED BY A Streptomyces STRAIN"", 《THE JOURNAL OF ANTIBIOTICS》 * |
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| CN109497077B (en) | 2020-11-24 |
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