CN109486894A - A kind of production method of Valine - Google Patents
A kind of production method of Valine Download PDFInfo
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- CN109486894A CN109486894A CN201910028347.XA CN201910028347A CN109486894A CN 109486894 A CN109486894 A CN 109486894A CN 201910028347 A CN201910028347 A CN 201910028347A CN 109486894 A CN109486894 A CN 109486894A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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Abstract
The invention discloses a kind of production methods of Valine, comprising: S1, fermentation procedure: composite bacteria being accessed in fermentation medium, continuously ferments, obtains valine fermentation liquid；S2, filtering；S3, ultrafiltration, concentration, crystallization: the filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is evaporated concentration, obtains concentrate；Dissolved agent is added into obtained concentrate and crystal grain is crystallized, filters drying, obtains valine crude product；S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization: the valine crude product that step S3 is obtained is dissolved in distilled water, high-purity valine is obtained through ion-exchange purification, decoloration, refined filtration, crystallization, the present invention is compared with prior art, fermentation period is short, the content of Valine is high, the yield of Valine is improved simultaneously, and product purity is high, provides technical support for China's Valine industrialized development.
Description
Technical field
The present invention relates to field of microbial fermentation, are related to a kind of production method of Valine, more specifically to one
The fermentation of kind Valine and the method isolated and purified.
Background technique
Valine (L-val), scientific name 2- amino -3 Methylbutanoic acid are one of big essential amino acids of human body eight, bright with L-
Propylhomoserin, l-Isoleucine are referred to as branched-chain amino acid, in medicine, flavouring, cosmetics, nutritional additive, feed addictive etc.
Aspect has a wide range of applications.
The production method of Valine mainly has extraction method, synthetic method and fermentation method, wherein microbe fermentation method has
Raw material cost is low, and reaction condition is mild and easily realizes the advantages that large-scale production, becomes the production method being widely used.But
It is that, currently, China is still immature with the technology of Production by Microorganism Fermentation Valine, fermentation level is not high, leads to fermentation week
Phase is long, and production acid is relatively low, and heteroacid is more, becomes the primary factor for influencing China's Valine industrial application.
CN101962664A discloses a kind of zymotechnique of efficiently production Valine, with auxotroph and figured silk fabrics ammonia
Sour leakage type bacterial strain is production bacterial strain, is configured to culture medium full of nutrition and balanced by a certain percentage with some nutrition compositions and makees
For fermentation medium, in fermentation process, dissolved oxygen is controlled in level appropriate by adjusting fermentor speed of agitator and air quantity, is led to
Overcurrent liquid feeding ammonia controls pH, and is controlled residual sugar in reduced levels by stream plus certain density glucose solution, after the 60h that ferments
The yield and conversion ratio of Valine increase substantially.
CN105695526A discloses a kind of zymotechnique that Valine production acid is improved by substep feed supplement, by training
The adjustment of based formulas is supported, and takes the mode of supplying technics at times and Discrete control dissolved oxygen simultaneously, promotes the life of valine
Object synthesis avoids the feedback regulation effect in amino acid biosynthetic pathway, reduces the generation of secondary acid, the zymotechnique energy
So that Valine is produced acid and the 50-55h production acid 7.0-8.0% of acid 4.5-5.5% raising till now, conversion are produced by original 70-80h
Rate is increased to 30-32% by original 20-25%.
Isolate and purify be Valine industrialized production another important procedure, in general, the cost Zhan isolated and purified is total
50% or more of production cost.Currently, the extracting method of Valine has the precipitation method, full embrane method and ion-exchange.The precipitation method
It is to be formed to precipitate according to the specific binding of precipitating reagent and Valine, then separation and Extraction, this method have high income, operation
The advantages that easy, but the technical problem high there are environmental pollution；Although full embrane method wastewater flow rate is few, membrane filter technique can only be removed largely
Inorganic ion and macromolecular foreign protein, can not root with amino acid similar in Valine property present in fermentation liquid
It removes, obtained Valine impurity is higher；Ion-exchange is that will remove the fermentation liquid tune pH of thallus to be acid, with highly acid sun
Ion exchange resin separates Valine and impurity amino acid, isolates Valine finally by ammonium hydroxide elution.If improving L-
Valine purity need to be extracted using the series connection of multipole ion exchange column, and it is larger that this will lead to production wastewater flow rate in production process, and adopts
It is relatively low with ion-exchange extract yield.
CN105274182A discloses a kind of technique of high efficiency extraction Valine from fermentation liquid, includes the following steps:
1) it ferments: by brevibacterium lactofermentus ATCC13869 seed liquor, Corynebacterium glutamicum ATCC21670 seed liquor and withered grass bud
Spore bacillus ATCC21616 seed liquor is mixed according to 2: 1: 1 volume ratio, is then linked into fermentation medium, is continuously fermented 60
Hour, obtain fermentation liquid；2) micro-filtrate membrane filtration: fermentation liquid uses micro-filtrate membrane filtration, collects filtered solution and trapped substance；The micro-filtration
Retaining molecular weight 5000MV；3) it is concentrated by evaporation: the filtered solution of step 2) being evaporated concentration, is concentrated into volume of the raw material liquid
One third collects concentrate；4) it crystallizes: the concentrate of step 3) being crystallized, Valine crude product is obtained；5) it dissolves:
Valine crude product addition deionized water toward step 4) re-dissolves to obtain Valine solution, and amount of water is crude product quality
40%-120%；6) ion exchange: by the Valine solution tune pH to 4.5 of step 5), 20-25 DEG C of temperature, strong acid ion is handed over
It changes resin and carries out ion exchange, then the weak aqua ammonia for being 2% with mass number parses, and collects efflux；7) ultrafiltration membrance filter: by step
6) gained efflux carries out ultrafiltration membrance filter, and molecular cut off 800MW collects filtrate；8) it is concentrated in vacuo: by the step 7) filter
Liquid is added in four-effect evaporator and is concentrated in vacuo, and is concentrated into 1/8th of filtrate volume；9) centrifugation drying: by step 8) institute
It obtains concentrate and carries out crystallisation by cooling, obtained Valine finished product is dried in centrifugation.
CN108285912 discloses a kind of method that pharmaceutical grade valine is extracted in fermentation preparation comprising: step 1) fermentation
Process: (1) continuously fermenting brevibacterium lactofermentus 50-55 hours according in the inoculum concentration access fermentation medium of 6-8%,
Obtain valine fermentation liquid；Temperature control in fermentation process is at 30 DEG C, and 6.5, control concentration of glucose is not less than for pH control
10g/L；(2) filtrate A and wet thallus are obtained using ceramic membrane filter valine fermentation liquid；1-3wt% electricity is added into wet thallus
Gas mountain flour, 100rpm stir 30min, then stop stirring, are heated to 50-55 DEG C, keep the temperature 60-90s, cooled to room temperature,
It is then added in the Dialysis culture base of three times weight, temperature control is at 30 DEG C, 100rpm stir culture 4-6h, ceramic membrane filter
Collect thallus and liquor B；Step 2) ultrafiltration and concentration prepare valine crude product: merging filtrate A obtained by step 1) and liquor B, lead to
It crosses ultrafiltration membrane and carries out ultrafiltration, collect ultrafiltrate, by ultrafiltrate by being concentrated in vacuo to valine content as 30wt%, cooling is tied
Crystalline substance, centrifugation obtain valine crude product；Step 3) acid is molten, crystallizes and is centrifuged: using the 7mol/L hydrochloric acid pair of 1.0-1.5 times of volume
It is molten that valine crude product carries out a hypo acid, then through decrease temperature crystalline, centrifugation, obtains valine solid A and mother liquor；Using 0.5-0.8 times
It is molten that the 1mol/L hydrochloric acid of volume carries out quadratic acid to valine solid A, then through decrease temperature crystalline, centrifugation, it is solid to obtain valine again
Body B and mother liquor；Step 4) is water-soluble, ion exchange, decoloration, refined filtration and crystallization: using the distilled water of 8-10 times of volume to figured silk fabrics ammonia
After sour solid B progress is water-soluble, pharmaceutical grade valine is obtained through ion exchange, decoloration, refined filtration, crystallization.But since valine exists
Solubility with temperature variation in pure water less when the present invention uses decrease temperature crystalline, still has a large amount of solutes molten when crystallizing and ending
In solvent, cause crystalline product yield low, the yield so as to cause valine product is lower.
Currently, China is still immature with the technology of Production by Microorganism Fermentation Valine, strain selection and breeding condition
It is unreasonable, cause fermentation period long, conversion ratio and production acid are relatively low, while existing separating and purifying technology falls behind, in crystallization process
Still there are a large amount of solutes to be dissolved in solvent, cause crystalline product yield low, leverages the yield of Valine, lead to China
The market competitiveness of domestic Valine is poor, cannot achieve large-scale production.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide a kind of production methods of Valine, from fermentation
It is optimized with two aspects are isolated and purified, while increasing Valine yield, improves yield and the production of Valine
Quality provides technical support for the production domesticization of Valine.
To achieve the goals above, the invention adopts the following technical scheme:
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
By composite bacteria brevibacterium flavum HL41 (CICC10135), candida tropicalis (Candida tropicalis)
DLPU-IL-In brevibacterium flavum JHV2-58 access fermentation medium, continuously ferments, obtain valine fermentation liquid；
S2, filtering:
It is added cycloheptaamylose in the valine fermentation liquid obtained to step S1, after stirring and dissolving, using ceramic membrane filter,
Obtain filtrate and wet thallus；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is evaporated concentration, is obtained
Concentrate；Dissolved agent is added into obtained concentrate and crystal grain is crystallized, filters drying, obtains valine crude product；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
The valine crude product that step S3 is obtained is dissolved in distilled water, is obtained through ion-exchange purification, decoloration, refined filtration, crystallization
Obtain high-purity valine.
Preferably, the inoculum concentration of composite bacteria is 4.0-6.0% in step S1.
Preferably, brevibacterium flavum HL41 (CICC10135), candida tropicalis in composite bacteria described in step S1
(Candida tropicalis)DLPU-IL-Mass ratio with brevibacterium flavum JHV 2-58 is 3:1:1.5.
Preferably, the component of fermentation medium described in step S1 include: glucose 10-15g/L, seawood meal 1-5g/L,
Corn pulp 5-10g/L, ammonium sulfate 15-35g/L, epsom salt 0.1-0.5g/L and potassium dihydrogen phosphate 0.5-1.2g/L, sterilizing
Preceding pH is 6.8-7.0.
Preferably, the condition of fermentation described in step S1 is that fermentation temperature is 32 ± 2 DEG C, pressure 0.103MPa, fermentation
Time is 40-50h.
Preferably, fermentation described in step S1 uses intermittent aeration, controls the dissolved oxygen content in culture medium, specifically
Are as follows:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated.
It is further preferred that the time for stopping aeration in step (2) is 20-90min.
Preferably, the mass ratio of valine fermentation liquid and cycloheptaamylose is 100:3-6 in step S2.
Preferably, the volume ratio of concentrate and former ultrafiltrate is 1:3-4 in step S3.
Preferably, crystallization described in step S3 are as follows: in 20-50min, with the rate of 1-10mL/min to 20-30 DEG C
Concentrate in be added and dissolve and wash away agent, while crystal grain is added, stirs, precipitate crystal.
It is further preferred that the agent that dissolves and washes away is at least two in methanol, ethyl alcohol, acetone and glycerine；More into one
Step is preferably the glycerine of volume ratio 2:1 and the mixed liquor of ethyl alcohol.
It is further preferred that the Valine that the crystal grain is 10-30 μm.
It is further preferred that the mass ratio of the concentrate and Valine is 500-1000:1.
Preferably, the mass ratio of valine crude product and distilled water is 1:3-5 in step S4.
The invention has the benefit that
(1) present invention optimizes in terms of fermenting and isolating and purifying two, shortens fermentation period, increases Valine
Content, while improving the yield of Valine, improve product quality, provided for China's Valine industrialized development
Technical support；
(2) present invention improves Valine by the compounding of different strain, the control of the optimization of culture medium and dissolved oxygen
Conversion ratio, shorten fermentation period, reduce the generation of miscellaneous amino acid；Pass through the intermittent aeration in fermentation process simultaneously,
So that strain is in the state of aerobic and micro- oxygen, promote the generation of valine, improves the conversion ratio of valine, while effectively keeping away
The generation of foam in fermentation process is exempted from.
(3) of the invention to isolate and purify that process is simple, and relative cost is low, meanwhile, by improving crystallization processes, avoid drop
The residual of valine in a solvent in temperature crystallization, improves the yield of valine product.
Specific embodiment
The present invention provides a kind of production methods of Valine, comprising the following steps:
S1, fermentation procedure:
By composite bacteria brevibacterium flavum HL41 (CICC10135), candida tropicalis (Candida tropicalis)
DLPU-IL-In brevibacterium flavum JHV2-58 access fermentation medium, continuously ferments, obtain valine fermentation liquid；
S2, filtering:
It is added cycloheptaamylose in the valine fermentation liquid obtained to step S1, after stirring and dissolving, using ceramic membrane filter,
Obtain filtrate and wet thallus；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is evaporated concentration, is obtained
Concentrate；Dissolved agent is added into obtained concentrate and crystal grain is crystallized, filters drying, obtains valine crude product；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
The valine crude product that step S3 is obtained, is dissolved in distilled water, obtains through ion-exchange purification, decoloration, refined filtration, crystallization
Obtain high-purity valine.
The present invention is not particularly limited the source of all raw materials, is commercially available.
The composite bacteria is brevibacterium flavum HL41 (CICC10135), candida tropicalis (Candida
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58, it is preferable that the brevibacterium flavum HL41 (CICC10135),
Candida tropicalis (Candida tropicalis) DLPU-IL-Mass ratio with brevibacterium flavum JHV 2-58 is 3:1:
1.5.The cut-in method of the composite bacteria is inoculation method well known to those skilled in the art, has no special limitation, institute
The inoculum concentration for stating composite bacteria is preferably 4.0-6.0%.The present invention utilizes the compounding of three kinds of strains, is improving fermentation and acid ability
While, the purity of Valine in fermentation liquid is improved, the production of high-purity Valine product is conducive to.
The fermentation medium includes 10-15g/L glucose, 1-5g/L seawood meal, 5-10g/L corn pulp, 15-35g/
L ammonium sulfate, 0.1-0.5g/L epsom salt and 0.5-1.2g/L potassium dihydrogen phosphate, pH is 6.8-7.0 before sterilizing, and is more preferably wrapped
Include 11-14g/L glucose, 1.5-4.5g/L seawood meal, 5.5-9g/L corn pulp, 14-34g/L ammonium sulfate, 0.15-0.45g/L
Epsom salt and 0.55-1.1g/L potassium dihydrogen phosphate, pH is 6.8-7.0 before sterilizing, and further preferably includes the Portugal 11.5-13.5g/L
Grape sugar, 1.6-4.4g/L seawood meal, 5.6-8.5g/L corn pulp, 14.5-33g/L ammonium sulfate, seven water sulfuric acid of 0.16-0.44g/L
Magnesium and 0.56-1.0g/L potassium dihydrogen phosphate, pH is 6.8-7.0 before sterilizing, and most preferably includes 12.5g/L glucose, the sea 2.8g/L
Algae powder, 8.4g/L corn pulp, 16.8g/L ammonium sulfate, 0.32g/L epsom salt and 0.76g/L potassium dihydrogen phosphate, pH before sterilizing
For 6.8-7.0.Above-described raw material is raw material well known to those skilled in the art, is had no specifically limited.
The fermentation use method well known to those skilled in the art, have no it is specifically limited, it is heretofore described
The temperature of fermentation is preferably 30-34 DEG C, and more preferably 30.5-33.5 DEG C, be further preferably 30.8-33.2 DEG C, the pressure of the fermentation
Power is 0.103MPa, and the time of the fermentation is preferably 40-50h, more preferably 41-49h, is further preferably 42-48h, most preferably
For 44h.
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated.
The present invention improves composite bacteria fermentation and produces by the way that composite bacteria culture medium and zymotechnique are optimized
Amount shortens fermentation period, while by the intermittent aeration in fermentation process, strain being made to be in the state of aerobic and micro- oxygen,
The generation for promoting valine reduces the generation of miscellaneous amino acid, improves the conversion ratio of valine, while effectively preventing sending out
The generation of foam during ferment.
Filtering of the present invention is specifically limited using having no well known to a person skilled in the art ceramic membrane filter,
Cycloheptaamylose is added in valine fermentation liquid before filtration, the mass ratio of preferably valine fermentation liquid and cycloheptaamylose is
100:3-6；More preferably 100:3.2-5.5；It is further preferably 100:3.5-5.0；Most preferably 100:4.2.
Using well known to a person skilled in the art technologies, it is special to have no for ultrafiltration, concentration and crystallization of the present invention
Limitation, crystallization optimum condition of the present invention are as follows: in 20-50min, with the rate of 1-10mL/min to 20-30 DEG C of concentration
It is added in liquid and dissolves and washes away agent, while crystal grain is added, stirred, precipitate crystal.The dissolved agent be preferably methanol, ethyl alcohol, acetone and
At least one of glycerine, more preferably glycerine and ethyl alcohol；The volume ratio of the glycerine and ethyl alcohol is preferably 2:1；Institute
Stating crystal grain is preferably Valine, and the partial size of the Valine is preferably 10-30 μm, more preferably 12-28 μm, further preferably for
14-26 μm, most preferably 18 μm；The mass ratio of the concentrate and Valine is 500-1000:1；More preferably
550-950:1 is further preferably 580-920:1, most preferably 678:1.The present invention adds dissolved agent and crystal grain in crystallization process,
Keep the crystal particle diameter being precipitated more uniform, avoids the residual of valine in a solvent in decrease temperature crystalline, can effectively improve figured silk fabrics
The yield of propylhomoserin product.
Water-soluble, ion exchange of the present invention, decoloration, refined filtration and crystallization be all made of well known to a person skilled in the art
Technology, has no specifically limited, and the mass ratio of valine crude product and distilled water is preferably 1:3- in step S4 of the present invention
5；More preferably 1:3.2-4.8 is further preferably 1:3.4-4.6, most preferably 1:5.3.
In order to further illustrate the present invention, with reference to embodiments to a kind of producer of Valine provided by the invention
Method is described in detail.
Reagent used in following embodiment is commercially available.
Embodiment 1
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 4.0% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium include 10g/L glucose, 1g/L seawood meal, 5g/L corn pulp, 15g/L ammonium sulfate,
0.1g/L epsom salt and 0.5g/L potassium dihydrogen phosphate, pH is 6.8 before sterilizing；The temperature of the fermentation is 30 DEG C, the hair
The pressure of ferment is 0.103MPa, and the time of the fermentation is 40h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:3, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/3 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 20min, it is added with the rate of 10mL/min into 20 DEG C of concentrate and dissolves and washes away agent,
Valine is added simultaneously as crystal grain, stirring precipitates crystal.The dissolved agent is the glycerine and second that volume ratio is 2:1
Alcohol；The partial size of the Valine is 10 μm, and the mass ratio of the concentrate and Valine is 500:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:3, the valine crude product that step S3 is obtained is dissolved in distilled water, through ion-exchange purification,
Decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.0% in fermentation liquid, and sugared conversion ratio is 46.5%, obtains the purity of Valine finished product
It is 99.92%, yield 78.5%.
Embodiment 2
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 6.0% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium include 15g/L glucose, 5g/L seawood meal, 10g/L corn pulp, 35g/L ammonium sulfate,
0.5g/L epsom salt and 1.2g/L potassium dihydrogen phosphate, pH is 7.0 before sterilizing；The temperature of the fermentation is 34 DEG C, the hair
The pressure of ferment is 0.103MPa, and the time of the fermentation is 50h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:6, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/4 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 50min, be added with the rate of 1mL/min into 30 DEG C of concentrate and dissolve and wash away agent, together
When be added Valine be used as crystal grain, stir, precipitate crystal.The dissolved agent is the glycerine and ethyl alcohol that volume ratio is 2:1；
The partial size of the Valine is 30 μm, and the mass ratio of the concentrate and Valine is 1000:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:5, the valine crude product that step S3 is obtained is dissolved in distilled water, through ion-exchange purification,
Decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.2% in fermentation liquid, and sugared conversion ratio is 47.8%, obtains the purity of Valine finished product
It is 99.90%, yield 79.5%.
Embodiment 3
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 4.5% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium includes 11g/L glucose, 1.5g/L seawood meal, 5.5g/L corn pulp, 14g/L sulfuric acid
Ammonium, 0.15g/L epsom salt and 0.55g/L potassium dihydrogen phosphate, pH is 6.9 before sterilizing；The temperature of the fermentation is 33.5 DEG C,
The pressure of the fermentation is 0.103MPa, and the time of the fermentation is 41h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:3.2, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/3 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 25min, be added with the rate of 8mL/min into 25 DEG C of concentrate and dissolve and wash away agent, together
When be added Valine be used as crystal grain, stir, precipitate crystal.The dissolved agent is the glycerine and ethyl alcohol that volume ratio is 2:1；
The partial size of the Valine is 12 μm, and the mass ratio of the concentrate and Valine is 550:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:3.2, the valine crude product that step S3 is obtained is dissolved in distilled water, pure through ion exchange
Change, decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.5% in fermentation liquid, and sugared conversion ratio is 48.5%, obtains the purity of Valine product
It is 99.95%, yield 80.1%.
Embodiment 4
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 5.8% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium include 14g/L glucose, 5.5g/L seawood meal, 9g/L corn pulp, 34g/L ammonium sulfate,
0.45g/L epsom salt and 1.1g/L potassium dihydrogen phosphate, pH is 7.0 before sterilizing；The temperature of the fermentation is 30.5 DEG C, described
The pressure of fermentation is 0.103MPa, and the time of the fermentation is 49h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:5.5, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/4 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 45min, be added with the rate of 3mL/min into 28 DEG C of concentrate and dissolve and wash away agent, together
When be added Valine be used as crystal grain, stir, precipitate crystal.The dissolved agent is the glycerine and ethyl alcohol that volume ratio is 2:1；
The partial size of the Valine is 28 μm, and the mass ratio of the concentrate and Valine is 950:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:4.8, the valine crude product that step S3 is obtained is dissolved in distilled water, it is pure through ion exchange
Change, decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.7% in fermentation liquid, and sugared conversion ratio is 48.7%, obtains the purity of Valine finished product
It is 99.94%, yield 85.1%.
Embodiment 5
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 5.5% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium includes 11.5g/L glucose, 1.6g/L seawood meal, 5.6g/L corn pulp, 14.5g/L sulphur
Sour ammonium, 0.16g/L epsom salt and 0.56g/L potassium dihydrogen phosphate, it is 30.8 that pH, which is the temperature of 6.8 fermentations, before sterilizing
DEG C, the pressure of the fermentation is 0.103MPa, and the time of the fermentation is 42h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:3.5, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/3 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 27min, it is added with the rate of 6.5mL/min into 24 DEG C of concentrate and dissolves and washes away agent,
Valine is added simultaneously as crystal grain, stirring precipitates crystal.The dissolved agent is the glycerine and second that volume ratio is 2:1
Alcohol；The partial size of the Valine is 14 μm, and the mass ratio of the concentrate and Valine is 580:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:3.4, the valine crude product that step S3 is obtained is dissolved in distilled water, it is pure through ion exchange
Change, decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.8% in fermentation liquid, and sugared conversion ratio is 48.9%, obtains the purity of Valine finished product
It is 99.93%, yield 86.5%.
Embodiment 6
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 4.4% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium includes 13.5g/L glucose, 4.4g/L seawood meal, 8.5g/L corn pulp, 33g/L sulfuric acid
Ammonium, 0.44g/L epsom salt and 1.0g/L potassium dihydrogen phosphate, pH is 6.9 before sterilizing, and the temperature of the fermentation is 33.2 DEG C,
The pressure of the fermentation is 0.103MPa, and the time of the fermentation is 48h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:5.0, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/4 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 43min, it is added with the rate of 4.7mL/min into 28 DEG C of concentrate and dissolves and washes away agent,
Valine is added simultaneously as crystal grain, stirring precipitates crystal.The dissolved agent is the glycerine and second that volume ratio is 2:1
Alcohol；The partial size of the Valine is 26 μm, and the mass ratio of the concentrate and Valine is 920:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:4.6, the valine crude product that step S3 is obtained is dissolved in distilled water, it is pure through ion exchange
Change, decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 9.9% in fermentation liquid, and sugared conversion ratio is 49.2%, obtains the purity of Valine product
It is 99.96%, yield 89.8%.
Embodiment 7
A kind of production method of Valine, comprising the following steps:
S1, fermentation procedure:
Brevibacterium flavum HL41 (CICC10135), the candida tropicalis (Candida for being 3:1:1.5 by mass ratio
tropicalis)DLPU-IL-With brevibacterium flavum JHV2-58 composite bacteria by 5.1% access fermentation medium, continuously send out
Ferment obtains valine fermentation liquid；
The fermentation medium includes 12.5g/L glucose, 2.8g/L seawood meal, 8.4g/L corn pulp, 16.8g/L sulphur
Sour ammonium, 0.32g/L epsom salt and 0.75g/L potassium dihydrogen phosphate, pH is 7.0 before sterilizing, and the temperature of the fermentation is 32.5
DEG C, the pressure of the fermentation is 0.103MPa, and the time of the fermentation is 44h；
Intermittent aeration is used in the fermentation process, controls the dissolved oxygen content in culture medium, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated；
S2, filtering:
By the mass ratio of 100:4.0, cycloheptaamylose, stirring and dissolving is added in the valine fermentation liquid that obtains to step S1
Afterwards, using ceramic membrane filter, filtrate and wet thallus are obtained；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is concentrated by evaporation to former ultrafiltration
The 1/4 of liquid product, obtains concentrate；Dissolved agent is added into obtained concentrate and Valine is crystallized, filters drying,
Obtain valine crude product；
The crystallization condition are as follows: in 38min, it is added with the rate of 5.3mL/min into 26 DEG C of concentrate and dissolves and washes away agent,
Valine is added simultaneously as crystal grain, stirring precipitates crystal.The dissolved agent is the glycerine and second that volume ratio is 2:1
Alcohol；The partial size of the Valine is 18 μm, and the mass ratio of the concentrate and Valine is 678:1；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
By the mass ratio of 1:5.3, the valine crude product that step S3 is obtained is dissolved in distilled water, it is pure through ion exchange
Change, decoloration, refined filtration, crystallization obtain high-purity valine.
The content of Valine is 10.5% in fermentation liquid, and sugared conversion ratio is 49.9%, obtains the pure of Valine finished product
Degree is 99.97%, yield 95.7%.
Comparative example 1
This comparative example and embodiment 7 the difference is that: the seawood meal in fermentation medium is replaced by soybean-cake flour.
The content of Valine is 6.5% in fermentation liquid, and sugared conversion ratio is 28.0%, obtains the purity of Valine finished product
It is 99.90%, yield 66.5%.
Comparative example 2
This comparative example and embodiment 7 the difference is that: dissolved oxygen is controlled using different oxygen supply in the fermentation process:
0-18h is 5-10% after being 20%, 18h.
The content of Valine is 6.8% in fermentation liquid, and sugared conversion ratio is 26.7%, obtains the purity of Valine finished product
It is 99.85%, yield 65.8%.
Comparative example 3
This comparative example and embodiment 7 the difference is that: do not add cycloheptaamylose in step S2.
The content of Valine is 10.3% in fermentation liquid, and sugared conversion ratio is 49.5%, obtains the pure of Valine finished product
Degree is 96.95%, yield 66.7%.
Comparative example 4
This comparative example and embodiment 7 the difference is that: in step S3 in crystallization process crystal grain Valine partial size
It is 8 μm, the mass ratio of concentrate and Valine is 450:1.
The content of Valine is 10.6% in fermentation liquid, and sugared conversion ratio is 49.8%, obtains the pure of Valine finished product
Degree is 96.22%, yield 65.2%.
Comparative example 5
This comparative example and embodiment 7 the difference is that: dissolved agent is ethyl alcohol in crystallization process in step S3.
The content of Valine is 10.7% in fermentation liquid, and sugared conversion ratio is 50.0%, and the purity for obtaining Valine is
95.98%, yield 62.9%.
Comparative example 6
This comparative example and embodiment 7 the difference is that: dissolved agent is glycerine in crystallization process in step S3.
The content of Valine is 10.3% in fermentation liquid, and sugared conversion ratio is 49.8%, obtains the pure of Valine finished product
Degree is 96.12%, yield 61.5%.
Comparative example 7
This comparative example and embodiment 7 the difference is that: the fermentation liquid that embodiment 7 is obtained uses CN108285912
The technique of embodiment 2 carries out separating-purifying, and Valine is made.
The content of Valine is 10.4% in fermentation liquid, and sugared conversion ratio is 49.5%, obtains the pure of Valine finished product
Degree is 99.87%, yield 63.2%.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art within the technical scope of the present disclosure, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of production method of Valine, which comprises the following steps:
S1, fermentation procedure:
By composite bacteria brevibacterium flavum HL41 (CICC10135), candida tropicalis (Candida tropicalis) DLPU-
IL-In brevibacterium flavum JHV 2-58 access fermentation medium, continuously ferments, obtain valine fermentation liquid；
S2, filtering:
It is added cycloheptaamylose in the valine fermentation liquid obtained to step S1, after stirring and dissolving, using ceramic membrane filter, obtains
Filtrate and wet thallus；
S3, ultrafiltration, concentration, crystallization:
The filtrate that step S2 is obtained carries out ultrafiltration, obtains ultrafiltrate；Obtained ultrafiltrate is evaporated concentration, is concentrated
Liquid；Dissolved agent is added into obtained concentrate and crystal grain is crystallized, filters drying, obtains valine crude product；
S4, water-soluble, ion exchange, decoloration, refined filtration and crystallization:
The valine crude product that step S3 is obtained, is dissolved in distilled water, obtains through ion-exchange purification, decoloration, refined filtration, crystallization high
Purity valine.
2. production method according to claim 1, which is characterized in that composite bacteria brevibacterium flavum described in step S1
HL41 (CICC10135), candida tropicalis (Candida tropicalis) DLPU-IL-With brevibacterium flavum JHV 2-58
Mass ratio be 3:1:1.5.
3. production method according to claim 2, which is characterized in that the group subpackage of fermentation medium described in step S1
It includes: glucose 10-15g/L, seawood meal 1-5g/L, corn pulp 5-10g/L, ammonium sulfate 15-35g/L, epsom salt 0.1-
0.5g/L and potassium dihydrogen phosphate 0.5-1.2g/L, pH is 6.8-7.0 before sterilizing.
4. production method according to claim 3, which is characterized in that the condition of fermentation described in step S1 includes fermenting
Temperature is 32+2 DEG C, pressure 0.103MPa, fermentation time 40-50h.
5. production method according to claim 4, which is characterized in that fermentation described in step S1 uses intermittent aeration,
The dissolved oxygen content in culture medium is controlled, specifically:
(1) it is aerated 2h using Roots blower, controls the oxygen degree of dissolution saturation in fermentation medium in 50-60%；
(2) Roots blower is closed, aeration is stopped, monitoring the dissolved oxygen in culture solution；
(3) when the oxygen degree of dissolution saturation in culture medium is down to 0, step (1)-(2) are repeated.
6. production method according to claim 1, which is characterized in that valine fermentation liquid and cycloheptaamylose in step S2
Mass ratio be 100:3-6.
7. production method according to claim 1-6, which is characterized in that crystallization described in step S3 are as follows:
It in 20-50min, is added with the rate of 1-10mL/min into 20-30 DEG C of concentrate and dissolves and washes away agent, while crystal grain is added, stirred,
It precipitates crystal.
8. production method according to claim 7, which is characterized in that the agent that dissolves and washes away is ethyl alcohol and glycerine.
9. production method according to claim 8, which is characterized in that the Valine that the crystal grain is 10-30 μm.
10. production method according to claim 9, which is characterized in that the mass ratio of the concentrate and Valine is
500-1000:1.
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| CN109943511A (en) * | 2019-04-28 | 2019-06-28 | 江南大学 | One plant of brevibacterium flavum for producing Valine and its application |
| CN110372528A (en) * | 2019-08-19 | 2019-10-25 | 精晶药业股份有限公司 | A kind of method of purification of valine |
| CN110643547A (en) * | 2019-11-07 | 2020-01-03 | 巴彦淖尔华恒生物科技有限公司 | Brevibacterium flavum for producing L-valine and method for producing L-valine by using same |
| CN113025667A (en) * | 2020-12-31 | 2021-06-25 | 安徽华恒生物科技股份有限公司 | Preparation method and application of efficient and environment-friendly amino acid fermentation medium |
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
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| CN109943511A (en) * | 2019-04-28 | 2019-06-28 | 江南大学 | One plant of brevibacterium flavum for producing Valine and its application |
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| CN110643547B (en) * | 2019-11-07 | 2022-11-08 | 巴彦淖尔华恒生物科技有限公司 | Brevibacterium flavum for producing L-valine and method for producing L-valine by using same |
| CN113025667A (en) * | 2020-12-31 | 2021-06-25 | 安徽华恒生物科技股份有限公司 | Preparation method and application of efficient and environment-friendly amino acid fermentation medium |
| CN113025667B (en) * | 2020-12-31 | 2022-10-25 | 安徽华恒生物科技股份有限公司 | Preparation method and application of amino acid fermentation medium |
| CN114015559A (en) * | 2021-09-08 | 2022-02-08 | 安徽华恒生物科技股份有限公司 | High-efficiency valine semi-continuous fermentation method and complete equipment thereof |
| CN114015559B (en) * | 2021-09-08 | 2023-06-23 | 安徽华恒生物科技股份有限公司 | Efficient valine semi-continuous fermentation method and complete equipment thereof |
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