CN109481477A - A kind of Preparation method and use of the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium - Google Patents
A kind of Preparation method and use of the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium Download PDFInfo
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- CN109481477A CN109481477A CN201811540536.7A CN201811540536A CN109481477A CN 109481477 A CN109481477 A CN 109481477A CN 201811540536 A CN201811540536 A CN 201811540536A CN 109481477 A CN109481477 A CN 109481477A
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- annesl
- mycoderma
- mycelia
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- alcohol extracting
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- 241000589220 Acetobacter Species 0.000 title claims abstract description 46
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 235000019441 ethanol Nutrition 0.000 claims abstract description 29
- 210000004881 tumor cell Anatomy 0.000 claims abstract description 20
- 230000000259 anti-tumor effect Effects 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims abstract description 10
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 9
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims abstract description 5
- 201000011510 cancer Diseases 0.000 claims abstract description 5
- 210000002919 epithelial cell Anatomy 0.000 claims abstract description 5
- 210000004907 gland Anatomy 0.000 claims abstract description 5
- 201000005296 lung carcinoma Diseases 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 5
- 239000000758 substrate Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims abstract description 3
- 239000000284 extract Substances 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 4
- 239000002246 antineoplastic agent Substances 0.000 claims description 3
- 230000001093 anti-cancer Effects 0.000 claims description 2
- 229940041181 antineoplastic drug Drugs 0.000 claims description 2
- 239000008194 pharmaceutical composition Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 229940079593 drug Drugs 0.000 abstract description 8
- 239000003814 drug Substances 0.000 abstract description 8
- 239000000469 ethanolic extract Substances 0.000 abstract description 8
- 240000000599 Lentinula edodes Species 0.000 abstract description 7
- 235000001715 Lentinula edodes Nutrition 0.000 abstract description 7
- 238000001914 filtration Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 244000251953 Agaricus brunnescens Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The present invention provides a kind of preparation methods of the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium, and lentinus edodes strain stick annesl mycelia mycoderma is removed from lentinus edodes strain stick, is then dried, crushed after being dried；Annesl mycelia mycoderma powder is weighed, ethyl alcohol is added, is extracted under 80 ~ 100 DEG C of water bath condition, repeats merging filtrate after extracting, then carries out filtering acquisition extracting solution；Extracting solution is placed under conditions of -90 ~ -50 DEG C and is freezed, then dry with vacuum freeze drier, concentration, obtain mushroom mycelium annesl mycelia mycoderma ethanol extract.The present invention also provides application of the ethanol extract of the annesl mycelia mycoderma of above-mentioned mushroom mycelium in preparation treatment antitumor cell drug or health-care products.Using human lung carcinoma cell spca-1 and gland cancer mankind's alveolar substrate epithelial cell A549 as target tumor, its antitumous effect is detected using external inhibition and kill tumour cell method, has and significantly inhibits effect.
Description
Technical field
The invention belongs to biomedicine fields, are related to a kind of mushroom, specifically a kind of annesl mycelia of mushroom mycelium
With the Preparation method and use of the ethanol extract of mycoderma.
Background technique
Antibacterials are the most wide a kind of drugs of clinical application, but there is many to ask for the application of this kind of drug at present
Topic, such as drug resistance, toxic side effect.Especially the resistance development of bacterium is than very fast, so as to serious sense caused by this kind of bacterium
Contaminating no effective drug can be used, and cause larger threat to human health.Therefore, people are developed from natural resources at present
An emphasis of the new antibacterials as antibacterials research.
Mushroom [Lentinula edodes (Berk.) Pegler.], is commonly called as dried mushroom, is second-biggest-in-the-world edible mushroom, plants
Stock is trained in China, has 800 years or more history so far.In 1989, Lentinus Edodes In China total output was more than Japan, a jump for the first time
As the first big country of world's Lentnus edodes.The anti-curing cancers of mushroom it is in extensive range, have been used for clinical treatment.There are many mushroom also contains
Vitamin, minerals, to human metabolism is promoted, improving organism adaptation power has great role.In actual production process, mushroom
Cultivation period can at least be divided into four important stages, and respectively vegetative hyphae growth phase, mycelia after-ripening and annesl forms rank
Section, former base formation stages, sporophore growth stage of development.So far, the bacteria stick surface layer of mushroom mycelium after-ripening and annesl formation stages
The specific effect of annesl mycoderma include having not been reported in terms of In Vitro Bacteriostatic.
Summary of the invention
The purpose of the present invention is to provide a kind of Preparation method and use of the annesl mycoderma alcohol extracting thing of mushroom mycelium, institutes
The Preparation method and use of the alcohol extracting thing of the annesl mycoderma for this mushroom mycelium stated will solve in the prior art for treating
The technical issues of drug of tumour has toxic side effect, while the annesl mycoderma of mushroom mycelium is not utilized effectively.
The present invention provides a kind of preparation methods of the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium, including walk as follows
It is rapid:
1) the annesl mycelia of mushroom mycelium and mycoderma are wrapped from Lenlinus edodes and is removed, is then dried, crushed after being dried
Cross 40-200 mesh；
2) weigh annesl mycelia mycoderma powder, in mass ratio the ratio for l:20~50 be added mass percent concentration be 60~
70% ethyl alcohol extracts 2~8 hours under 80~100 DEG C of water bath condition, repeats to extract 3~6 times, then merging filtrate carries out
It filters, obtains extracting solution；
3) it is freezed under conditions of extracting solution being placed in -90~-50 DEG C 12-24 hours, it is then dry with vacuum freeze drier
It is dry, concentration, obtain mushroom mycelium annesl mycelia mycoderma alcohol extracting thing.
The present invention also provides the annesl mycelia mycoderma alcohol extracting things of the mushroom mycelium obtained by above-mentioned method.
The present invention also provides the annesl mycelia mycoderma alcohol extracting things of above-mentioned mushroom mycelium to prepare anti-tumor drug, resist
Application in tumor reagent, Anticancer Food or anti-tumor healthcare product.
Further, the tumour cell is human lung carcinoma cell spca-1 or gland cancer mankind's alveolar substrate epithelial cell
A549。
The present invention also provides a kind of pharmaceutical compositions, the annesl mycelia mycoderma alcohol extracting containing above-mentioned mushroom mycelium
Object.
The present invention using the annesl mycelia mycoderma of discarded cultivating champignon bacterium bag as raw material, crushed by scraping, hot water extracts,
(suction filtration), drying and other steps is concentrated under reduced pressure, obtains a kind of second of mushroom mycelium annesl mycoderma with high anti-tumor activity effect
Alcohol extracting thing.With human lung carcinoma cell spca-1 and gland cancer mankind's alveolar substrate epithelial cell A549 for tested tumour cell, use
It is external that tumour growth and kill tumour cell method is inhibited to detect its antitumous effect, the results show that mushroom mycelium
Annesl mycoderma ethanol extract have stronger anti-human lung carcinoma cell spca-1 and gland cancer mankind's alveolar substrate epithelial cell
A549 effect.Its antitumor cell effect is compared with the mycelia of non-annesl of mushroom mycelium and the ethanol extract of mycoderma,
Have the effect of significantly inhibiting tumour cell.The annesl mycoderma extract of lentinus edodes strain stick of the invention can be used as improve immunity and
The raw material of antitumor class pharmaceutical preparation, is made tablet, capsule, compound preparation etc., is applied to the industries such as food, drug.
The present invention is compared with prior art, its technical effect is that actively and apparent.The present invention is with the annesl of mushroom mycelium
Mycelia and mycoderma are raw material, carry out the development of antineoplastic extract, can be used as natural food and drug antitumor agent, are realized
The annesl mycelia of mushroom and the comprehensive utilization of mycoderma.
Detailed description of the invention
Fig. 1 shows that mushroom mycelium annesl mycoderma and non-annesl mycelia alcohol extracting thing act on lower tumour cell A549 survival rate
With the comparative effectiveness of fatality rate.
Fig. 2 shows that mushroom mycelium annesl mycoderma and non-annesl mycelia alcohol extracting thing act on lower tumour cell spca-1 survival
The comparative effectiveness of rate and fatality rate.
Specific embodiment
Beneficial effects of the present invention are further proved below by way of activity of tumor cells experiment is inhibited:
Embodiment 1
1) the annesl mycelia of mushroom mycelium and mycoderma are wrapped from Lenlinus edodes and is removed, is then dried, crushed after being dried
Cross 40-200 mesh；
2) weigh 20g annesl mycelia mycoderma powder, be added 1000ml mass percent concentration be 70% ethyl alcohol, 80~
It is extracted 4 hours under 100 DEG C of water bath condition, repeats to extract 5 times, then merging filtrate is filtered, extracting solution is obtained；
3) it is freezed under conditions of extracting solution being placed in -90~-50 DEG C 12 hours, then with vacuum freeze drier dry 24
Hour, concentration, obtain mushroom mycelium annesl mycelia mycoderma alcohol extracting thing.
Inhibiting effect of the annesl mycelia mycoderma alcohol extracting thing to tumour cell of mushroom mycelium is measured using mtt assay.Wherein
The ethanol extract of annesl mycelia mycoderma, and the non-annesl as control mycelia mycoderma ethanol extract setting gradient
For 10,100,1000 μ g/ml.
Steps are as follows:
1) logarithmic phase cell is collected to be digested and be collected by centrifugation, removed when cell grows to density and is about 80-90%
2ml culture medium is added in supernatant, is uniformly mixed it；
2) adjusting concentration of cell suspension, (cell concentration is 5-10 × 104A/ml), 100ul (96 orifice plate rims are added in every hole
32 holes are filled with sterile PBS, because quickly, drug is easily concentrated for moisture evaporation in the hole at edge, are influenced on experiment big)；
3) 5%CO2, 37 DEG C are incubated for 24 hours, until cell monolayer is paved with bottom hole (96 hole flat underside)；
4) culture solution in hole is first sucked, the ethanol extract of the mushroom mycelium mycoderma of concentration gradient is added, extract
Concentration gradient: three repetitions, 5%CO is arranged in each concentration of 0 μ g, 10 μ g, 100 μ g, 1000 μ g2, 37 DEG C are incubated for 24 hours, are inverted
Microscopically observation；(before adding sample protein, albumen will be filtered degerming)
5) 10ulMTT solution (5mg/ml, i.e. 0.5%MTT) is added in every hole, continues to cultivate 4h；
6) culture is terminated, culture solution in hole is carefully sucked；
7) 150ulMTT is added in every hole, sets low-speed oscillation 3min on shaking table, dissolves crystal sufficiently；
8) in enzyme-linked immunosorbent assay instrument OD570Nm and OD630nmPlace measures the light absorption value in each hole.
Experimental result is as follows:
Inhibit the test result of tumour cell A549, survival rate and the fatality rate effect of tumour cell are respectively such as 1 He of table
Shown in Fig. 1.Inhibit the test result of tumour cell spca-1, survival rate and the fatality rate effect of tumour cell are respectively such as table 2
With shown in Fig. 2.
1 mushroom mycelium annesl mycoderma of table is compared with non-annesl mycelia alcohol extracting thing is to tumour cell A549 survival rate
By table 1 and Fig. 1 it is found that the alcohol extracting thing of relatively non-annesl mycelia, the alcohol extracting thing pair of lentinus edodes strain stick annesl mycoderma
Tumour cell A549 has preferable antitumous effect, is 1000ug/ml to the preferable concentration to tumour cell A549.
2 mushroom mycelium annesl mycoderma of table is compared with non-annesl mycelia alcohol extracting thing is to tumour cell spca-1 survival rate
By table 2 and Fig. 2 it is found that the alcohol extracting thing of relatively non-annesl mycelia, the alcohol extracting thing pair of lentinus edodes strain stick annesl mycoderma
Tumour cell spca-1 has preferable antitumous effect, is 1000ug/ml to the preferable concentration to tumour cell spca-1.
Claims (5)
1. a kind of preparation method of the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium, it is characterised in that include the following steps:
1) the annesl mycelia of mushroom mycelium and mycoderma are wrapped from Lenlinus edodes and is removed, is then dried, crushed after being dried crosses 40-
200 meshes；
2) annesl mycelia mycoderma powder is weighed, it is 60~70% that mass percent concentration, which is added, in the ratio for l:20~50 in mass ratio
Ethyl alcohol, extract 2~8 hours under 80~100 DEG C of water bath condition, repeat to extract 3~6 times, then merging filtrate is taken out
Filter obtains extracting solution；
3) it is freezed under conditions of extracting solution being placed in -90~-50 DEG C 12-24 hours, it is then dry with vacuum freeze drier, it is dense
Contracting, obtain mushroom mycelium annesl mycelia mycoderma alcohol extracting thing.
2. the annesl mycelia mycoderma alcohol extracting thing for passing through the mushroom mycelium that method of claim 1 obtains.
3. the annesl mycelia mycoderma alcohol extracting thing of mushroom mycelium as claimed in claim 2 is preparing anti-tumor drug, antitumor examination
Application in agent, Anticancer Food or anti-tumor healthcare product.
4. application according to claim 3, which is characterized in that the tumour cell be human lung carcinoma cell spca-1 or
Gland cancer mankind's alveolar substrate epithelial cell A549.
5. a kind of pharmaceutical composition, which is characterized in that the annesl mycelia mycoderma alcohol extracting containing mushroom mycelium as claimed in claim 2
Take object.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811540536.7A CN109481477B (en) | 2018-12-17 | 2018-12-17 | Preparation method and application of color-conversion hypha pericarp alcohol extract of mushroom hypha |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811540536.7A CN109481477B (en) | 2018-12-17 | 2018-12-17 | Preparation method and application of color-conversion hypha pericarp alcohol extract of mushroom hypha |
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| Publication Number | Publication Date |
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| CN109481477A true CN109481477A (en) | 2019-03-19 |
| CN109481477B CN109481477B (en) | 2021-08-06 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2119909B1 (en) * | 1970-12-30 | 1975-06-06 | Iizuka Chiyokichi | |
| CA2352512A1 (en) * | 1998-11-27 | 2000-06-08 | Kobayashi Pharmaceutical Co., Ltd. | Lak activity enhancers derived from extract of lentinus endodes mycelium and lak activity-enhancers formulations containing the extract |
| CN107823227A (en) * | 2016-10-11 | 2018-03-23 | 上海市农业科学院 | A kind of preparation method and applications of the annesl mycoderma extract of lentinus edodes strain stick |
-
2018
- 2018-12-17 CN CN201811540536.7A patent/CN109481477B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2119909B1 (en) * | 1970-12-30 | 1975-06-06 | Iizuka Chiyokichi | |
| CA2352512A1 (en) * | 1998-11-27 | 2000-06-08 | Kobayashi Pharmaceutical Co., Ltd. | Lak activity enhancers derived from extract of lentinus endodes mycelium and lak activity-enhancers formulations containing the extract |
| CN107823227A (en) * | 2016-10-11 | 2018-03-23 | 上海市农业科学院 | A kind of preparation method and applications of the annesl mycoderma extract of lentinus edodes strain stick |
Non-Patent Citations (1)
| Title |
|---|
| 李利华: "香菇不同部位醇提物的抗氧化活性", 《食品与发酵工业》 * |
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