CN109479903A - A kind of Java Isaria bacterium conidium oil-suspending agent and its application - Google Patents
A kind of Java Isaria bacterium conidium oil-suspending agent and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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Abstract
The invention belongs to biological pesticide and biological control of insect pests technical field, it is related to conidium oil-suspending agent and its application of a kind of Java Isaria Isariajavanica.Java Isaria bacterium conidium oil-suspending agent of the invention includes the component of following mass percent: the pure powder 3~10% of the conidium of Java Isaria bacterium, dispersing agent 3~10%, thickener 3~10%, oil base 80~90%.Java Isaria bacterium of the invention belongs to mycota Fungi on taxology, double-core suberathem Dikarya, Ascomycota Ascomycota, cup fungi subphylum Pezizomycotina, excrement shell Gammaproteobacteria Sordariomycetes, meat seat bacterium subclass Hypocreomycetidae, Hypocreales Hypocreales, cordyceps sinensis Cordycepps Cordycipitaceae, Isaria Pseudomonas Isaria.Oil-suspending agent provided by the invention is up to 80% or more to the pathogenicity of the third-instar larvae of prodenia litura for preventing and treating prodenia litura.
Description
Technical field
The invention belongs to biological pesticides and biological control of insect pests technical field, are related to a kind of fungus insecticide, and in particular to
The conidium oil-suspending agent of Java Isaria Isariajavanica a kind of and its application.
Background technique
Java Isaria Isariajavanica is a kind of common insect pathogenic fungus, extensive in distributed in nature, to people,
Poultry not will cause environmental pollution without safety, causes insect to fall ill and dead from the intrusion of pest epidermis, is to have " similar action of contace poison "
Biological pesticide, insect resistance will not be caused, thus this bacterium has obtained more and more concerns, have and develop into fungi desinsection
The potentiality of agent.However, fungus insecticide is because itself be life entity, field sensitive to environmental factors such as high temperature, arid and ultraviolet lights
Between using being restricted, it is therefore desirable to the conidium of fungi is processed into dosage form appropriate, to improve its Field information effect.
Oil-suspending agent is that a kind of insoluble soild pesticidal active ingredient in oil based solvent is dispersed in non-aqueous media i.e. oils
In, the stable suspension liquid dosage form of high degree of dispersion is formed by surfactant, main component includes pesticide activity component, oil
Base, dispersing agent, emulsifier and thickener etc..Fungus conidium oil-suspending agent has following advantages: medium is generally natural
Oils, non-environmental-pollution, will not generate dust at the problems such as can be avoided inflammable and explosive and poisoning in production, than great, packaging
Small in size, dispersibility and plate-out ability are all relatively good, are easier to be adhered to insect and plant surface, resistance of rainwater washing against, and drug effect is higher.Cause
And fungus conidium oil-suspending agent is increasingly taken seriously.The some patents of invention disclosed at present are related to the mitogenetic spore of fungi
Seed oil suspending agent:
Chinese invention patent: a kind of fungal spore oil suspending agent (application number 200810069704.9) discloses including white
The preparation method of stiff bacterium, green muscardine fungus, Lecanicillium lecanii, Trichoderma etc..
A kind of Chinese invention patent: Paecilomyces lilacinus fungal spore oil suspending agent and its preparation method and application (application number
201210256521.4)。
A kind of Chinese invention patent: Trichoderma oil-suspending agent and preparation method thereof (application number 201610905079.1).
Although oil-suspending agent has a variety of advantages, also successfully overcome fungal spore be difficult to use in dry environment,
The defect that insecticidal effect is influenced by humidity in environment, but oil-suspending agent has the following aspects: (1) formulation oils at
This is higher, and when field rotating medicine need to be not suitable for industrial production with spraying after oil or dedicated diluted；(2) entomogenous fungi spore
Germination rate in formulation oils is low, and storage stability is poor；These greatly constrain the use of entomogenous fungi.Therefore, it is developed into
This low, excellent storage stability, small fungal oil mafic novel form affected by environment become the hot spot of fungi preparation research.
Summary of the invention
The purpose of the present invention is combining entomogenous fungi and oil-suspending agent, provide a kind of application at low cost, user
Just entomogenous fungi conidium oil-suspending agent.
It is a further object of the present invention to provide the preparation methods of above-mentioned oil-suspending agent.
Another object of the present invention is to provide application of the oil-suspending agent in terms of preventing and treating crop pests, specifically,
Application of the oil-suspending agent in terms of preventing and treating prodenia litura is provided.
To achieve the above object, the technical scheme adopted by the invention is that:
A kind of Java Isaria bacterium conidium oil-suspending agent, the component including following mass percent: Java cluster
The pure powder 3~10% of the conidium of spore bacterium, dispersing agent 3~10%, thickener 3~10%, oil base 80~90%.
Preferably, the pure powder 5% of the conidium of Java Isaria bacterium, dispersing agent 5%, thickener 5%, oil base 85%.
Preferably, the pure powder spore content of the Java Isaria conidium is >=1.5 × 1010A spore/g, water content≤
3%, spore rate >=95% living.
Preferably, the ratio of the dispersing agent and thickener is 1:1.
Preferably, the dispersing agent is selected from fat alcohol polyethylene ether, sodium methylene bis-naphthalene sulfonate, rosin acid, dodecyl
One or more of sodium sarcosinate, sodium butylnaphthalenesulfonate.
Preferably, the thickener is selected from one or both of xanthan gum, aluminum stearate.
Preferably, the oil base is selected from one or more of ready-mixed oil, peanut oil, soybean oil, corn oil.
It is highly preferred that the dispersing agent is fat alcohol polyethylene ether；The thickener is xanthan gum or aluminum stearate；It is described
Oil base is corn oil.
The preparation method of the oil-suspending agent: by the pure powder of conidium of above-mentioned Java Isaria bacterium and dispersing agent, thickening
Agent mixing, is then added oil base, mixed mixed liquor is placed in concussion instrument and is sufficiently shaken up, so that the dispersion of conidia powder and addition
Agent, thickener are fully dispersed in oil base to be opened, and the conidium oil-suspending agent of Java Isaria bacterium is made.
Application of the above-mentioned Java Isaria bacterium conidium oil-suspending agent in terms of preventing and treating prodenia litura is provided.
Compared with prior art, beneficial effects of the present invention are as follows:
(1) Java Isaria bacterium conidium oil-suspending agent provided by the invention is store under room temperature and -4 DEG C of low temperature environments
Deposit that stability is good, storage period is long；Particularly preferably it is formulated the pure powder 5% of conidium, the fatty alcohols polyethylene of Java Isaria bacterium
Ether 5%, xanthan gum 5%, corn oil 85%, three months stability of storage is best at normal temperature, another optimization formula Isaria
The pure powder 5% of the conidium of bacterium, fat alcohol polyethylene ether 5%, aluminum stearate 5%, corn oil 85%, under -4 DEG C of low temperature environments
It is best to store three months stability.
(2) oil-suspending agent activity provided by the invention is good, and toxic effect is high；For preventing and treating prodenia litura, indoor virulence
After the third-instar larvae of processing prodenia litura, the death rate increases as time increases, and preventive effect is up to 80% or more.
(3) oil-suspending agent preparation method of the invention is simple, and products material is from a wealth of sources, is suitble to industrial production.
(4) Java Isaria bacterium conidium oil-suspending agent provided by the present invention can be used for field control evil on a large scale
Worm.
Detailed description of the invention
The 1-28 conidium oil-suspending agent after 100d is stored under Fig. 1 room temperature；
The 1-8 conidium oil-suspending agent after 100d is stored under Fig. 2-4 DEG C of low temperature；
Specific embodiment
In order to keep the objectives, technical solutions, and advantages of the present invention more concise, present invention following specific embodiments
It is illustrated, however, the present invention is by no means limited to these examples.The following is only the preferred embodiment of the present invention, is used only for
The description present invention, should not be understood as limiting the scope of the present invention.It should be pointed out that all in the spirit and principles in the present invention
Within any modifications, equivalent replacements, and improvements etc. done, should all be included in the protection scope of the present invention.Therefore, this hair
The scope of protection shall be subject to the appended claims for bright patent.
Embodiment 1: the preparation and quality testing of the pure powder of Java Isaria bacterium conidium
1, the pure powder, preparation method thereof of Java Isaria bacterium conidium
(1) level-one solid culture, from Java, Isaria slant strains take conidium to be inoculated in sterilized PDA (Ma Ling
Potato 200g, glucose 20g, agar 20g, water 1000mL) on plate, is cultivated 2 weeks or so in 25 DEG C of incubators, scrape mitogenetic spore
Son, it is spare.
(2) secondary liquid culture takes the conidium of above-mentioned culture, and it is molten to be suspended in sterile 0.02% (v/v) Tween-80
In liquid, it is inoculated in equipped in sterilized PD fluid nutrient medium (potato 200g, glucose 20g, water 1000mL), 25 ±
1 DEG C, 170r/min shake culture 48h is spare as seed liquor.
(3) three-level rice fermentation produces spore culture, after rice is impregnated 8h with clear water, places into polypropylene plastics pocket, often
Bag 400g, autoclave sterilization, every bag of above-mentioned seed liquor 50mL of inoculation, dark condition cultivate 14d at 25 ± 1 DEG C, then use spore
Molecular separator collects spore to obtain the pure powder of Java Isaria bacterium conidium, and 4 DEG C of refrigerator shorts are spare.
2, the quality testing of the pure powder of Java Isaria bacterium conidium
(1) determination of spore content
The pure powder of conidium of 10mg is accurately weighed in 1mL 0.02% (v/v) Tween-80 solution, is sufficiently mixed
It is even, certain multiple is diluted, spore concentration is measured using blood counting chamber, is repeated 3 times, calculates the spore content of conidia powder, as a result
Such as table 1.
(2) measurement of spore water content
Spore water content is measured using oven drying method: taking a clean weighing bottle dry in 120 DEG C of drying boxes
10min, weigh to obtain bottle weight m1 after its cooling；It weighs 1g conidia powder and is placed in weighing bottle the m2 that weighs to obtain；120 DEG C are placed it in again
2h is dried in drying box, is covered bottle cap and is weighed to obtain m3, calculates spore water content, be averaged after being repeated 5 times, it is aqueous to calculate spore
Amount, as a result such as table 1.
(3) measurement of conidia germination rate
Weigh 1mg conidia powder, be added the autoclaved 0.05%Tween-80 aqueous solution eddy mixer of 10mL by its
It is sufficiently mixed uniformly, records spore count under microscope, spore suspension is diluted, so that containing no more than 100 in every 10 μ l
10 μ l are connected on PDA plate by spore count, and the number that fungi sprouts on PDA is observed after three days, calculate spore germination rate, are repeated
Three times, it is averaged, as a result such as table 1.
The quality testing of the 1 pure powder of Java Isaria bacterium conidium of table
The data of table 1 are shown, are 1.73 × 10 by the spore powder content that rice fermentation method obtains10Spore/g, it is aqueous
Amount is that 3.03%, 72h spore rate living is 95.46%, can carry out subsequent oil-suspending agent research
Embodiment 2: the screening of Java Isaria bacterium conidium oil-suspending agent auxiliary agent
For trying conidia powder: the pure powder of conidium prepared by embodiment 1.
For trying dispersing agent: sodium methylene bis-naphthalene sulfonate, rosin acid, sarcosyl, sodium butylnaphthalenesulfonate, fat
Alcohol polyvinylether is purchased from Guangzhou Ke Hao Biotechnology Co., Ltd.
For trying thickener: xanthan gum, aluminum stearate are purchased from Guangzhou Ke Hao Biotechnology Co., Ltd.
For formation testing base: ready-mixed oil, peanut oil, soybean oil, corn oil.
1, the preparation of oil-suspending agent
1.5mL centrifuge tube is taken, the 5% pure powder of Java Isaria bacterium conidium gathered is added, 5% dispersion is then added
Agent and 5% thickener are eventually adding 85% oil base polishing to 1mL, are placed in concussion instrument and sufficiently shake up, so that conidia powder and addition
Auxiliary agent it is fully dispersed in oil base open, share 28 kinds of formulas, each formula is arranged six repetitions, under room temperature and -4 DEG C of each three pipes
It places respectively, in 28 kinds of formulas, the selection of dispersing agent, thickener, oil base asks that see Table 2 for details and table 3.
2, the character detection of oil-suspending agent
(2) storage stability
The oil-suspending agent hoverheight that above-mentioned 28 kinds of formulas have stored 100d under room temperature and -4 DEG C of environment is measured, is determined high
It is " +++ " that degree, which is less than its storage stability of 1.00cm, and storage stability is " ++++" highly within the scope of 1.0cm-1.50cm, high
Storage stability of the degree greater than 1.50cm is " +++ ++ ", wherein "+" more multilist shows that storage stability is better.28 kinds formula in
The results are shown in attached figure 1-2 for storage stability at room temperature and-4 DEG C, wherein 1-28 indicates to store 100d under room temperature；1 ' -28 ' indicate -4
100d is stored under DEG C environment.Converting "+" for the storage stability of attached drawing 1-2 indicates, the results are shown in Table 2 and table 3.
(1) measurement of conidia germination rate
The above-mentioned oily suspension formulation prepared is shaken uniformly again, takes 1mL finish to dilute 1000 times, uses eddy mixer
It is sufficiently mixed uniformly, records spore count under microscope, spore suspension is diluted, be no more than so that containing in every 10 μ l
10 μ l are connected on PDA plate by 100 spore counts, and the number of conidia germination on PDA is observed after three days, are calculated spore and are sprouted
Hair rate is averaged in triplicate, and the pure conidia powder any auxiliary agent is not added the results are shown in Table 4 as blank control.
The storage stability of each group oil-suspending agent storage 100d under 2 room temperature of table
The storage stability of (- 4 DEG C) each group oil-suspending agent storage 100d under 3 low temperature of table
The storage stability of oil-suspending agent determines by measuring its hoverheight, attached drawing 1-2's the results show that in room temperature
In 0.849-1.801 etc., the oil-suspending agent height stored under -4 DEG C of low temperature exists the oil-suspending agent hoverheight of lower storage
1.079-1.838 etc., determining that height is less than its storage stability of 1.00cm is " +++ ", highly in 1.0cm-1.50cm range
Interior storage stability is " ++++", and highly the storage stability greater than 1.50cm is " +++ ++ ".
Show the better conclusion of storage stability according to "+" more multilist, by Java cluster it can be seen from the result of table 2 and table 3
In 28 kinds of formulas of spore bacterium conidium oil-suspending agent, the storage stability that 100d is stored under room temperature and -4 DEG C of environment is relatively good
Have 10 kinds of formulas, be the pure powder of the Java number 2:5% Isaria bacterium conidium, 85% ready-mixed oil, 5% rosin acid, 5% respectively
Xanthan gum；The pure powder of the Java number 6:5% Isaria bacterium conidium, 85% peanut oil, 5% sarcosyl, 5% Huang
Virgin rubber；The pure powder of the Java number 6:5% Isaria bacterium conidium, 85% ready-mixed oil, 5% rosin acid, 5% xanthan gum；Number 8:
The pure powder of 5% Java Isaria bacterium conidium, 85% soybean oil, 5% rosin acid, 5% xanthan gum；The Java number 14:5% cluster
The pure powder of spore bacterium conidium, 85% ready-mixed oil, 5% fat alcohol polyethylene ether, 5% xanthan gum；The Java number 16:5% Isaria
The pure powder of bacterium conidium, 85% ready-mixed oil, 5% fat alcohol polyethylene ether, 5% aluminum stearate；The Java number 18:5% Isaria
The pure powder of bacterium conidium, 85% peanut oil, 5% fat alcohol polyethylene ether, 5% xanthan gum；The Java number 20:5% Isaria bacterium
The pure powder of conidium, 85% peanut oil, 5% fat alcohol polyethylene ether, 5% aluminum stearate；The Java number 24:5% Isaria bacterium
The pure powder of conidium, 85% soybean oil, 5% fat alcohol polyethylene ether, 5% aluminum stearate；The Java number 26:5% Isaria bacterium
The pure powder of conidium, 85% corn oil, 5% fat alcohol polyethylene ether, 5% xanthan gum；The Java number 28:5% Isaria bacterium point
The pure powder of life spore, 85% corn oil, 5% fat alcohol polyethylene ether, 5% aluminum stearate.
The conidia germination rate of 4 each group oil-suspending agent of table
The conidia germination rate of Java Isaria bacterium conidium oil-suspending agent in 28 kinds of formulas, can by the result of table 4
Know, the conidia germination rate for the Java Isaria bacterium conidium oil-suspending agent stored at normal temperature, compared with the control, sprouts
The higher formula of rate is the pure powder of the Java number 26:5% Isaria bacterium conidium, 85% corn oil, 5% fatty alcohols polyethylene
Ether, 5% xanthan gum, under the formula, conidial germination rate is up to 94.27%, higher than the 86.38% of control；At -4 DEG C
The conidia germination rate for the Java Isaria bacterium conidium oil-suspending agent stored under environment, compared with the control, germination rate compared with
High formula is the pure powder of the Java number 28:5% Isaria bacterium conidium, 85% corn oil, 5% fat alcohol polyethylene ether, 5%
Aluminum stearate, under the formula, conidial germination rate is up to 87.78%, the germination rate 89.52% slightly less than compareed.
In conjunction with table 2 and table 4 as a result, storing that 100d rear stability is good, and Java Isaria bacterium conidium oil is outstanding under room temperature
The high formula of the conidia germination rate of floating agent be the pure powder of the Java number 26:5% Isaria bacterium conidium, 85% corn oil,
5% fat alcohol polyethylene ether, 5% xanthan gum.
In conjunction with table 3 and table 4 as a result, storing that 100d rear stability is good, and Java Isaria bacterium conidium oil is outstanding at -4 DEG C
The high formula of the conidia germination rate of floating agent be the pure powder of the Java number 28:5% Isaria bacterium conidium, 85% corn oil,
5% fat alcohol polyethylene ether, 5% aluminum stearate.
Embodiment 3: the experiment of Java Isaria bacterium conidium oil-suspending agent indoor virulence and field control effectiveness test
1, for trying insect:
Prodenia litura, adult pick up from Guangzhou Agricultural University Of South China farm, are raised and are laid eggs with mulberry leaf, select after its hatching
It grows consistent 3 instar larvae and carries out indoor toxicity test.
2, for test preparation:
Processing group: being formulated according to 28 kinds, prepares 5% Java Isaria bacterium conidium oil-suspending agent 1mL, is added
100mL0.02%Tween-80 aqueous solution is mixed well with whirlpool concussion instrument, is configured to 1.0 × 10 with blood counting chamber adjusting7
The conidial suspension of a/mL；Control group: pure conidia powder concentration is 1.0 × 107The suspension of a/mL；Using clear water as blank
Control.
3, processing method:
It is equal that the Java Isaria bacterium conidium oil-suspending agent for being placed on 100d under the conditions of room temperature and -4 DEG C is taken out into concussion
It is even, it is configured to 1 × 107A/mL conidial suspension concentration, every cephalont, which is put into configured corresponding bacterium solution, impregnates 10s,
It is put into 5 third-instar larvaes in each culture dish, is put into 14:10 (L:D), 25 ± 1 DEG C, 70 ± 10% illumination box of relative humidity
Middle culture.Polypide situation is checked under anatomical lens every 12h, nymph soma is flat, color becomes withered and yellow person and is considered as death, each processing
It is repeated 3 times, the larva for impregnating tween is blank control group, calculates the death rate of prodenia litura, the results are shown in Table 5 and table 6.
The case fatality rate of prodenia litura after each group oil-suspending agent is handled under 5 room temperature of table
The cause of 28 kinds of formula Java Isaria bacterium conidium oil-suspending agent processing prodenia lituras of 100d is stored under room temperature
Dead rate is as shown in table 5, and data result shows the 5d to 9d after processing, and the third-instar larvae death rate of prodenia litura is with the time
Increase and increase, 9d after processing, the death rate of control group is 86.67%, and processing group is higher than or is equivalent to the dead of control group
The formula for dying rate has a number 13,14,15,16,17,26,28, the death rate is 86.67% respectively, 100%, 93.33%,
86.67%, 86.67%, 86.67%, 93.33%.
The death rate of prodenia litura after each group oil-suspending agent is handled at 6-4 DEG C of table
The cause of 28 kinds of formula Java Isaria bacterium conidium oil-suspending agent processing prodenia lituras of 100d is stored at -4 DEG C
Dead rate is as shown in table 6, and data result shows the 5d to 9d after processing, and the third-instar larvae death rate of prodenia litura is with the time
Increase and increase, 9d after processing, the death rate of control group is 86.67%, and processing group only has the death rate of number 26,28 to reach
To 80%.
In summary: having prepared 28 kinds of different Java Isaria bacterium oil-suspending agents, room temperature and -4 DEG C of each three weights herein
It is multiple, its suspension situation of observation is taken out after being placed 100d, it is found that different formula spore suspension situations has notable difference, low temperature
Condition storage stability is higher.In conjunction with the storage stability, spore germination rate and bioactivity of each group oil-suspending agent, two groups are obtained
The best oil-suspending agent of effect is number 26 and 28 respectively, and formula is respectively: Java Isaria bacterium conidium 5%, corn
Oily 85%, fat alcohol polyethylene ether 5%, xanthan gum 5%；Java Isaria bacterium conidium 5%, corn oil 85%, fatty alcohol
Polyvinylether 5%, aluminum stearate 5%.
Based on the above description of the preferred embodiments of the present invention, through the above description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
It is not limited to the contents of the specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (9)
1. a kind of Java Isaria bacterium conidium oil-suspending agent, which is characterized in that the component including following mass percent:
The pure powder 3~10% of the conidium of Java Isaria bacterium, dispersing agent 3~10%, thickener 3~10%, oil base 80~90%；Its
In, the Java Isaria bacterium belongs to mycota Fungi, double-core suberathem Dikarya, Ascomycota on taxology
Ascomycota, cup fungi subphylum Pezizomycotina, excrement shell Gammaproteobacteria Sordariomycetes, meat seat bacterium subclass
Hypocreomycetidae, Hypocreales Hypocreales, cordyceps sinensis Cordycepps Cordycipitaceae, Isaria Pseudomonas
Isaria。
2. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the Java cluster
The pure powder 5% of the conidium of spore bacterium, dispersing agent 5%, thickener 5%, oil base 85%.
3. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the Java cluster
The pure powder spore content of the conidium of spore bacterium is >=1.5 × 1010A spore/g, percentage of impurity≤0.1% water content≤3%, spore rate living
>=95%.
4. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the dispersing agent with
The ratio of thickener is 1:1.
5. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the dispersing agent choosing
One from fat alcohol polyethylene ether, sodium methylene bis-naphthalene sulfonate, rosin acid, sarcosyl, sodium butylnaphthalenesulfonate
Kind is several.
6. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the thickener choosing
From one or both of xanthan gum, aluminum stearate.
7. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the oil base is selected from
One or more of ready-mixed oil, peanut oil, soybean oil, corn oil.
8. Java Isaria bacterium conidium oil-suspending agent according to claim 1, it is characterised in that: the dispersing agent is
Fat alcohol polyethylene ether；The thickener is xanthan gum or aluminum stearate；The oil base is corn oil.
9. Java Isaria bacterium conidium oil-suspending agent described in any one claim is being prevented and treated according to claim 1~8
Application in terms of prodenia litura.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110839652A (en) * | 2019-11-15 | 2020-02-28 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof |
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| CN106489932A (en) * | 2016-10-17 | 2017-03-15 | 上海万力华生物科技有限公司 | A kind of trichoderma oil-suspending agent and preparation method thereof |
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| CN110839652A (en) * | 2019-11-15 | 2020-02-28 | 韶关市华实现代农业创新研究院 | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof |
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