CN109457688B - Coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition - Google Patents
Coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition Download PDFInfo
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- CN109457688B CN109457688B CN201811304804.5A CN201811304804A CN109457688B CN 109457688 B CN109457688 B CN 109457688B CN 201811304804 A CN201811304804 A CN 201811304804A CN 109457688 B CN109457688 B CN 109457688B
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- 239000002689 soil Substances 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 26
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 title claims abstract description 24
- 244000005700 microbiome Species 0.000 title claims abstract description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 title claims abstract description 12
- 230000008021 deposition Effects 0.000 title claims abstract description 11
- 230000003014 reinforcing effect Effects 0.000 title claims description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 34
- 238000011065 in-situ storage Methods 0.000 claims abstract description 9
- 238000005728 strengthening Methods 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 42
- 230000000694 effects Effects 0.000 claims description 12
- 239000002562 thickening agent Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 10
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 7
- 229910001424 calcium ion Inorganic materials 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- 229920001817 Agar Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 239000002245 particle Substances 0.000 abstract description 9
- 230000002787 reinforcement Effects 0.000 abstract description 7
- 239000011148 porous material Substances 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000035699 permeability Effects 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 101000965313 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Aconitate hydratase A Proteins 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
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- Engineering & Computer Science (AREA)
- Structural Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Agronomy & Crop Science (AREA)
- Mining & Mineral Resources (AREA)
- Paleontology (AREA)
- Civil Engineering (AREA)
- General Engineering & Computer Science (AREA)
- Processing Of Solid Wastes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一种基于微生物诱导碳酸钙沉积的加固粗粒土方法，包括在粗粒土试样中加入粘稠细菌溶液进行细菌固定；加入含有尿素、钙离子的处理液对完成细菌固定的粗粒土进行胶结处理。本发明将MICP技术首次应用于大粒径、大孔隙的粗粒土，改善了粗粒土力学性能的同时还保持了良好的透水性，与传统的混凝土加固技术相比，该发明生态环保，工期较短，工艺简单，造价较低，且可实现原位处理，在工程领域具有广泛的应用前景。The invention discloses a method for strengthening coarse-grained soil based on microorganism-induced calcium carbonate deposition. The method includes adding a viscous bacterial solution to a coarse-grained soil sample for bacterial fixation; Coarse-grained soil is cemented. The invention applies the MICP technology to the coarse-grained soil with large particle size and large pores for the first time, which improves the mechanical properties of the coarse-grained soil and maintains good water permeability. Compared with the traditional concrete reinforcement technology, the invention is ecological and environmentally friendly. The construction period is short, the process is simple, the cost is low, and in-situ processing can be realized, and it has wide application prospects in the engineering field.
Description
Technical Field
The invention relates to a coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition (MICP), and belongs to the field of foundation reinforcement.
Background
Coarse-grained soil foundation in engineering practice often has the function of draining water, but because the particles are loose, the particle size is larger, the physical and mechanical properties are poor, the anti-scouring capability and the stability are poor, and manual reinforcement is often needed. However, most of the pores of the traditional concrete reinforcement can be filled, so that the drainage performance of the coarse-grained soil foundation is poor, and the engineering requirements cannot be met.
The solidification of soil by microbial cementing is a novel soil reinforcing mode which is formed in recent years. The current MICP technology makes great progress in the field of sandy soil and silty soil with good permeability, has good scour resistance and soil body strength, and can meet certain engineering requirements. However, the size of the pores of the soil particles influences the retention, adsorption and transmission movement of microorganisms among the soil particles, and thus the popularization of MICP in the field of coarse-grained soil with larger grain size is restricted. In the field of coarse-grained soil, microorganisms are difficult to fix in particle pores, most of the microorganisms are washed away along with the pouring of a treatment liquid, and calcium carbonate crystals generated by a large amount of reaction are concentrated on the upper surfaces of particles and cannot play a role in cementation, so that the cementation efficiency is greatly reduced, and at present, no perfect coarse-grained soil reinforcing method exists.
Disclosure of Invention
In order to solve the technical problems, the invention provides a coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition.
In order to achieve the purpose, the invention adopts the technical scheme that:
a coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition comprises the following steps,
adding a viscous bacterial solution into the coarse-grained soil sample to perform bacterial immobilization;
adding a treating fluid containing urea and calcium ions to perform cementation treatment on the coarse-grained soil subjected to bacterial immobilization.
A viscous agent which does not affect the activity of bacteria is added to the bacterial solution to form a viscous bacterial solution.
The bacteria are fixed by in-situ fixing or stirring.
The thick bacterial solution for bacterial immobilization should submerge the coarse-grained soil.
Standing for a period of time after the bacteria are fixed, and draining the redundant viscous bacteria solution.
After the cementation treatment, if the coarse-grained soil does not achieve the preset strengthening effect, the bacteria fixation and the cementation treatment are sequentially carried out again until the preset strengthening effect is achieved.
When the bacteria are fixed again, the content of the thickening agent in the viscous bacteria solution is lower than that in the previous viscous bacteria solution.
Except for the first bacterial fixation, the viscous bacterial solution does not submerge coarse-grained soil during the subsequent bacterial fixation, and only in-situ fixation can be adopted.
The concrete process of the cementing treatment is that,
1) leveling coarse-grained soil with fixed bacteria;
2) adding the treating fluid into the leveled coarse-grained soil, wherein the treating fluid does not submerge the coarse-grained soil;
3) standing for a period of time, discharging and collecting the treatment solution;
4) and (3) measuring whether the calcium ion concentration in all collected treatment liquid reaches a set threshold value, if not, turning to the step (2), otherwise, finishing the primary cementation treatment.
The invention achieves the following beneficial effects: the MICP technology is applied to coarse-grained soil with large grain diameter and large pore space for the first time, the mechanical property of the coarse-grained soil is improved, and meanwhile, good water permeability is kept.
Detailed Description
The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
A coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition comprises the following steps:
step 1, a viscous bacterial solution is prepared.
Adding a thickening agent which does not influence the activity of bacteria into the bacterial solution to form a viscous bacterial solution, wherein the content of the thickening agent is not higher than 20 g/L, the permeation speed of the bacterial solution in the coarse-grained soil after the thickening agent is added is 0.03-0.05 cm/s, the duration time of the permeation is not lower than 1h, and the specific addition amount of the thickening agent is comprehensively determined according to the permeation speed, the duration time of the permeation and the cementation degree of the coarse-grained soil.
The thickening agent has water solubility, if the thickening agent is soluble at high temperature, the thickening agent is added into the culture medium before the bacteria inoculation, and the time of high temperature and high pressure is not less than 30 minutes; if the thickening agent is soluble at normal temperature, it is added to the bacterial solution after completion of the bacterial culture.
The viscous bacteria solution is prepared by performing activity control on a blank control group without adding a viscous agent, wherein the influence of the viscous agent on the bacterial activity cannot exceed 10% of the bacterial activity, and agar, sodium alginate and the like can be used as the viscous agent.
And 2, adding a viscous bacteria solution into the coarse-grained soil sample for bacteria immobilization. The thick bacterial solution for bacterial immobilization should submerge the coarse-grained soil.
The particle size range of the coarse-grained soil is 5-20 mm, and the coarse-grained soil can be one or mixture of calcareous soil, siliceous soil or recycled aggregate.
The bacteria are fixed by in-situ fixing or stirring.
The in-situ fixation method can add the viscous bacteria solution into coarse-grained soil by grouting, guniting, spraying and other methods, make use of the low permeability of the viscous bacteria solution to realize retention and adsorption of the viscous bacteria solution in the slow permeation process, and collect the permeated viscous bacteria solution for increasing the fixation and adsorption effect, and perform circulation treatment.
The stirring method is to add coarse-grained soil to be reinforced into an open container, add viscous bacteria solution and stir to wrap the coarse-grained soil on the surface of coarse-grained soil particles, and stir for not less than 5 minutes.
And 3, standing for a period of time after the bacteria are fixed, and draining redundant viscous bacteria solution. The actual standing time is adjusted according to the fluidity of the viscous bacterial solution, and the bacterial solution is slowly drained before the fluidity of the viscous bacterial solution is reduced.
And 4, adding a treatment solution containing urea and calcium ions to perform cementation treatment on the coarse grained soil subjected to bacterial immobilization.
The concentration of the treatment liquid is not higher than 1.5 mol/L, and the time of primary cementing treatment is not lower than 12 hours.
The concrete process of the cementing treatment is as follows:
1) the coarse-grained soil with fixed bacteria is leveled, and the hollow phenomenon is avoided.
2) And adding the processing liquid into the leveled coarse-grained soil, wherein the coarse-grained soil is prevented from being directly washed in the adding process, and the processing liquid is not submerged in the coarse-grained soil and is preferably added to a position 3-5 mm below the surface coarse-grained soil.
3) Standing for a period of time, discharging and collecting the treatment solution;
4) all collected process fluids were measured (all collected process fluids here refer to: the treatment fluid is added for a plurality of times and then the collected treatment fluid is discharged altogether) whether the calcium ion concentration reaches the set threshold value, the threshold value is 80 percent of the calcium ion concentration added, if not, the step 2 is carried out, otherwise, the one-time cementation treatment is finished.
And 5, after the cementation treatment, measuring the reinforcement effect in situ by using instruments such as a surface penetrometer and the like, and if the coarse-grained soil does not reach the preset reinforcement effect, sequentially carrying out bacterial fixation and cementation treatment again until the preset reinforcement effect is reached.
When the bacteria are fixed again, the content of the thickening agent in the viscous bacteria solution is lower than that in the previous viscous bacteria solution. Except for the first time of bacterium fixation, when subsequent bacteria are fixed, the viscous bacterium solution does not submerge coarse-grained soil, and the viscous bacterium solution is preferably added to a position 3-5 mm below the surface layer coarse-grained soil, and meanwhile, in-situ fixation can be adopted only.
The method applies the MICP technology to coarse-grained soil with large grain diameter and large pore space for the first time, improves the mechanical property of the coarse-grained soil and keeps good water permeability.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A coarse-grained soil reinforcing method based on microorganism-induced calcium carbonate deposition is characterized by comprising the following steps: comprises the following steps of (a) carrying out,
adding a viscous bacterial solution into a coarse-grained soil sample with the grain size of 5-20 mm for bacterial immobilization; adding a thickening agent which does not influence the activity of bacteria into the bacteria solution to form a viscous bacteria solution, wherein the thickening agent is agar or sodium alginate;
adding a treating fluid containing urea and calcium ions to perform cementation treatment on the coarse-grained soil subjected to bacterial immobilization;
after the cementation treatment, if the coarse-grained soil does not achieve the preset strengthening effect, sequentially carrying out bacterial fixation and cementation treatment again until the preset strengthening effect is achieved; when the bacteria are fixed again, the content of the viscous agent of the viscous bacteria solution is lower than that of the viscous agent of the previous viscous bacteria solution; the thick bacterial solution should submerge the coarse-grained soil when the bacteria are fixed for the first time, except for the bacteria are fixed for the first time, the thick bacterial solution does not submerge the coarse-grained soil when the bacteria are fixed for the subsequent time, and meanwhile, the in-situ fixation can only be adopted.
2. The coarse soil reinforcing method based on microorganism-induced calcium carbonate deposition according to claim 1, wherein: the first bacterial fixation adopts in-situ fixation or stirring method.
3. The coarse soil reinforcing method based on microorganism-induced calcium carbonate deposition according to claim 1, wherein: standing for a period of time after the bacteria are fixed, and draining the redundant viscous bacteria solution.
4. The coarse soil reinforcing method based on microorganism-induced calcium carbonate deposition according to claim 1, wherein: the concrete process of the cementing treatment is that,
1) leveling coarse-grained soil with fixed bacteria;
2) adding the treating fluid into the leveled coarse-grained soil, wherein the treating fluid does not submerge the coarse-grained soil;
3) standing for a period of time, discharging and collecting the treatment solution;
4) and (3) measuring whether the calcium ion concentration in all collected treatment liquid reaches a set threshold value, if not, turning to the step (2), otherwise, finishing the primary cementation treatment.
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| CN109900880A (en) * | 2019-03-25 | 2019-06-18 | 贵州大学 | A kind of MICP test method using immobilized microorganism technology |
| CN110055950A (en) * | 2019-04-01 | 2019-07-26 | 中国科学院寒区旱区环境与工程研究所 | A kind of device and method using microorganism bubble processing seasonal frozen ground frost heave ground |
| CN111441337B (en) * | 2020-04-30 | 2022-03-18 | 华中科技大学 | A kind of microbe-induced mineralization reinforcement soil grouting method by introducing urease inhibitor |
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| JPH11241335A (en) * | 1998-02-25 | 1999-09-07 | Raito Kogyo Co Ltd | Construction method of horizontal vein and improvement method of soft ground |
| JP4981457B2 (en) * | 2007-01-09 | 2012-07-18 | 電気化学工業株式会社 | Cement composition, injection material using the same, and method of using the same |
| CN101759438A (en) * | 2010-01-15 | 2010-06-30 | 东南大学 | Method for laminating on surface of cement-based material by using sodium alginate-supported bacterial strain |
| CN101936151A (en) * | 2010-09-10 | 2011-01-05 | 天津亿利科石油技术发展有限公司 | Method for profile control and oil displacement by immobilizing microorganisms |
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