CN109456931A - The directional proliferation method of probiotics preparation - Google Patents
The directional proliferation method of probiotics preparation Download PDFInfo
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- CN109456931A CN109456931A CN201811602287.XA CN201811602287A CN109456931A CN 109456931 A CN109456931 A CN 109456931A CN 201811602287 A CN201811602287 A CN 201811602287A CN 109456931 A CN109456931 A CN 109456931A
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- probiotics
- probiotics preparation
- pure natural
- bacillus
- proliferation method
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- Biomedical Technology (AREA)
- Virology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of directional proliferation methods of probiotics preparation, contain in the step of method: using the lignocellulosic hydrolysis nutritional agents rich in prebiotics as main nutrient composition, yeast powder and water are that auxiliary nutritional ingredient prepares pure natural microbiological culture media, with Bacillus subtillis, bacillus licheniformis and Bacillus acidi lactici in pure natural microbiological culture media, 3 kinds of aerobic probiotics are rised in value under aerobic conditions into probiotics preparation.
Description
Technical field
The present invention relates to a kind of directional proliferation methods of probiotics preparation, are related to technical field of microbe application.
Background technique
Currently, China produces 800,000,000 tons of crop materials every year, it is typically used for burning or burning in the ground, this is not only tight
Weight waste of resource, while environment is polluted again.Main component of the lignocellulosic as stalk, hydrolyzable are made rich in prebiotics
Lignocellulosic hydrolyzes nutritional agents, for aerobic water process, carries out the orientation fermentation proliferation of aerobic water process probiotics, fermentation benefit
Raw bacterium cell can be improved the function factor and nutrient of its water treatment efficiency by purification and concentration, cooperation, be made efficiently,
Green, environmentally friendly nutritional complexing agent product.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of directional proliferation method of probiotics preparation, its natural peaces
Entirely, low in cost, environmentally protective.
In order to solve the above technical problems, the technical scheme is that a kind of directional proliferation method of probiotics preparation,
Contain in the step of method:
Using the lignocellulosic hydrolysis nutritional agents rich in prebiotics as main nutrient composition, yeast powder and water are auxiliary nutritional ingredient
Pure natural microbiological culture media is prepared, with Bacillus subtillis, bacillus licheniformis and cream in pure natural microbiological culture media
Acidfast bacilli, 3 kinds of aerobic probiotics are rised in value under aerobic conditions into probiotics preparation.
Further, in the pure natural microbiological culture media, lignocellulosic hydrolyzes nutritional agents 20g, yeast powder 10g, water
2000ml。
Further, the pure natural microbiological culture media is nature PH, the steam high-voltage sterilizing 20min at 115 DEG C
Afterwards, 25 DEG C are cooled to.
Further, the Bacillus subtillis, bacillus licheniformis and Bacillus acidi lactici are to have activated probiotics.
Further, the Bacillus subtillis, bacillus licheniformis and Bacillus acidi lactici are seeded to described pure with 1% inoculum concentration
Natural microbial culture medium.
Further, 18h is cultivated on 37 DEG C of shaking tables after aerobic probiotics is seeded on natural microbial culture medium.
After above-mentioned technical proposal, pure natural microbiological culture media is using lignocellulosic as main nutrient composition, ferment
Female powder and water are auxiliary nutritional ingredient, other any chemical substances are not added, lignocellulosic and yeast powder are pure natural safety
Material, and containing abundant prebiotics in lignocellulosic, yeast powder contains good protein, complete B family vitamin, 14 kinds of rare mines
Material element and good dietary fiber.So culture medium of the invention is natural safety, full of nutrition, probiotics system obtained
Agent more safety and environmental protection, effect are more preferable.
Specific embodiment
In order that the present invention can be more clearly and readily understood, below according to specific embodiment, to the present invention make into
One step detailed description.
By Bacillus subtillis, bacillus licheniformis and Bacillus acidi lactici take lignocellulosic water by an activation acquirement kind liquid
Nutritional agents 20g is solved, yeast powder 10g, distilled water 2000ml prepare pure natural microbiological culture media, the vapor injection at 115 DEG C
Sterilize 20min, and Bacillus subtillis, bacillus licheniformis are then added after pure natural microbiological culture media is cooled to 25 DEG C
With lactobacillus species liquid, inoculum concentration is 1%, then under the conditions of temperature is 37 DEG C, cultivates 18h on shaking table, benefit can be made
Probiotics preparation, Bacillus subtillis bacteria concentration reach 2 × 10 9 cfu/mL, and Bacillus licheniformis concentration reaches 6 × 10 9
Cfu/mL, Bacillus acidi lactici bacteria concentration reach 3 × 108cfu/mL.
Aerobic proliferation of probiotics rate can be improved 30% by the method for the present embodiment, reach purpose of design.
Particular embodiments described above, pair present invention solves the technical problem that, technical scheme and beneficial effects carry out
It is further described, it should be understood that the above is only a specific embodiment of the present invention, is not limited to this
Invention, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this hair
Within bright protection scope.
Claims (6)
1. a kind of directional proliferation method of probiotics preparation, it is characterised in that contain in the step of method:
Using the lignocellulosic hydrolysis nutritional agents rich in prebiotics as main nutrient composition, yeast powder and water are auxiliary nutritional ingredient
Pure natural microbiological culture media is prepared, with Bacillus subtillis, bacillus licheniformis and cream in pure natural microbiological culture media
Acidfast bacilli, 3 kinds of aerobic probiotics are rised in value under aerobic conditions into probiotics preparation.
2. the directional proliferation method of probiotics preparation according to claim 1, it is characterised in that: the pure natural microorganism
In culture medium, lignocellulosic hydrolyzes nutritional agents 20g, yeast powder 10g, water 2000ml.
3. the directional proliferation method of probiotics preparation according to claim 1, it is characterised in that: the pure natural microorganism
Culture medium is nature PH, at 115 DEG C after steam high-voltage sterilizing 20min, is cooled to 25 DEG C.
4. the directional proliferation method of probiotics preparation according to claim 1, it is characterised in that: the Ko subtilis bar
Bacterium, bacillus licheniformis and Bacillus acidi lactici are to have activated probiotics.
5. the directional proliferation method of probiotics preparation according to claim 1, it is characterised in that: the Ko subtilis bar
Bacterium, bacillus licheniformis and Bacillus acidi lactici are seeded to the pure natural microbiological culture media with 1% inoculum concentration.
6. the directional proliferation method of probiotics preparation according to claim 1, it is characterised in that: aerobic probiotics is seeded to
18h is cultivated on 37 DEG C of shaking tables after on natural microbial culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811602287.XA CN109456931A (en) | 2018-12-26 | 2018-12-26 | The directional proliferation method of probiotics preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811602287.XA CN109456931A (en) | 2018-12-26 | 2018-12-26 | The directional proliferation method of probiotics preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109456931A true CN109456931A (en) | 2019-03-12 |
Family
ID=65614818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811602287.XA Pending CN109456931A (en) | 2018-12-26 | 2018-12-26 | The directional proliferation method of probiotics preparation |
Country Status (1)
| Country | Link |
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| CN (1) | CN109456931A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762250A (en) * | 2015-03-30 | 2015-07-08 | 华南师范大学 | Method for producing probiotics by utilizing lignocellulose hydrolysate |
| CN108497174A (en) * | 2017-02-23 | 2018-09-07 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus subtilis and produces fermented feed |
-
2018
- 2018-12-26 CN CN201811602287.XA patent/CN109456931A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104762250A (en) * | 2015-03-30 | 2015-07-08 | 华南师范大学 | Method for producing probiotics by utilizing lignocellulose hydrolysate |
| CN108497174A (en) * | 2017-02-23 | 2018-09-07 | 丽水学院 | A method of solid state fermentation being carried out to wood fibre hydrolysis raw material using bacillus subtilis and produces fermented feed |
Non-Patent Citations (1)
| Title |
|---|
| ELIZABETH M. PROSEN等: "Microbial utilization of levoglucosan in wood pyrolysate as a carbon and energy source", 《BIOTECHNOL. BIOENG.》 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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Application publication date: 20190312 |