CN109456886A - A kind of full-automatic nucleic acid signal amplification detection automatic work station - Google Patents
A kind of full-automatic nucleic acid signal amplification detection automatic work station Download PDFInfo
- Publication number
- CN109456886A CN109456886A CN201811523158.1A CN201811523158A CN109456886A CN 109456886 A CN109456886 A CN 109456886A CN 201811523158 A CN201811523158 A CN 201811523158A CN 109456886 A CN109456886 A CN 109456886A
- Authority
- CN
- China
- Prior art keywords
- sample
- couveuse
- nucleic acid
- board
- microwell plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of full-automatic nucleic acid signal amplification detection automatic work station, including working station, the first couveuse is provided in the working station, first couveuse is used to carry out hybridization incubation to sample of nucleic acid；Second couveuse, second couveuse are used to carry out calculating to sample of nucleic acid signal amplification and are incubated for；Board-washing machine, the board-washing machine are used to carry out board-washing operation to by the sample of nucleic acid of hybridization incubation；Interpretoscope, the interpretoscope are used to analyze by calculating the sample of nucleic acid that signal amplification is incubated for；Sample arm, moving operation of the sample arm for realizing sample of nucleic acid between the first couveuse, the second couveuse, board-washing machine and interpretoscope；It further include the controller for controlling sample arm, the first couveuse, the second couveuse, board-washing machine, interpretoscope；The present invention has the advantages that easy to use, integrated height, detection efficiency are high, intelligentized.
Description
Technical field
The invention belongs to nucleic acid signal amplification detection technical fields, and in particular to a kind of full-automatic nucleic acid signal amplification detection
Automatic work station.
Background technique
The predecessor of QuantiMAT technology is branched chain DNA signal amplification technique (branched DNA, bDNA), and bDNA is
A kind of nucleic acid for not depending on PCR amplification that Chiron company (first being purchased after Bayer corporate buyout by Siemens Company) releases
Hybridization signal amplification detection technology.
The technology overcomes the defects of traditional real time round pcr and uncertain factor, is not necessarily to extracting and purifying
RNA is not necessarily to reverse transcription, is not necessarily to PCR amplification, as long as after mixing the sample with the cracking of specific cleavage liquid, amplifying through probe hybridization with signal
After can obtain gene quantification result rapidly.
BDNA technology has many advantages, such as that highly sensitive, detection range is big and accurate quantitative analysis, to various common samples and qPCR
Being difficult the blood sample of analysis, the FFPE sample of paraffin embedding is same has with the formaldehyde fixation that mRNA height after many years is degraded is saved
There are high accuracy and reproducibility.
BDNA technology has been developed to the third generation at present, and technology is continued to optimize, and the combination of electrochemical techniques, nanotechnology etc.
The development prospect of the technology is more extended, bDNA technology is applied not only to viral DNA or the detection of RNA at present, it can also be used to detect
The expression of genes within cells, using more and more extensive in clinical detection and research work.
However, the existing work for being all based on room temperature or 37 degree of reaction temperatures based on chemiluminescent Automatic Workstation
Make platform, and the reaction temperature condition based on nucleic acid signal amplifying technique is then 55 degree, conventional reaction work station can not
While guarantee meets reaction temperature, the liquid of reaction system does not evaporate；Also, current also not ready-made platform can be completed
Nucleic acid hybridization signal amplifies integrated work.
Summary of the invention
A kind of high, detection efficiency easy to use, integrated is provided the purpose of the present invention is overcome the deficiencies in the prior art
High, intelligentized full-automatic nucleic acid signal amplification detection automatic work station.
Technical scheme is as follows:
A kind of full-automatic nucleic acid signal amplification detection automatic work station, including working station, in the working station
It is provided with
First couveuse, first couveuse are used to carry out hybridization incubation to sample of nucleic acid；
Second couveuse, second couveuse are used to carry out calculating to sample of nucleic acid signal amplification and are incubated for；
Board-washing machine, the board-washing machine are used to carry out board-washing operation to by the sample of nucleic acid of hybridization incubation；
Interpretoscope, the interpretoscope are used to analyze by calculating the sample of nucleic acid that signal amplification is incubated for；
Sample arm, the sample arm in the first couveuse, the second couveuse, board-washing machine and are sentenced for realizing sample of nucleic acid
Read the moving operation between instrument；
It further include controller, the controller moves sample of nucleic acid according to the program of setting for controlling sample arm
Dynamic, for controlling the first couveuse, the second couveuse is incubated for sample of nucleic acid, for control board-washing machine to nucleic acid samples into
Row board-washing tests and analyzes sample of nucleic acid for controlling interpretoscope.
Further, the working station is provided with the groove for placing interpretoscope.
Further, the couveuse lid being used cooperatively with the second couveuse is placed on the working station.
Further, the sample arm includes: sample-adding arm body, and the sample-adding arm body is equipped with lateral displacement device, longitudinal
Gearshift, telescopic displacement device, for carrying out the position of all directions to sample arm arm body under the control of the controller
It moves.
Further, the sample-adding arm body is connect with the working station by rotary electric machine, and the sample arm is whole
The angle of inverted V shaped structure, the inverted V-shaped structure can change under the control of the controller.
Further, the end of the sample arm is equipped with clamping part, for clamping the luminous microwell plate for placing sample of nucleic acid.
Further, the end of the sample arm is equipped with electromagnet, correspondingly, for placing the luminous microwell plate of sample of nucleic acid
Corresponding position be equipped with magnetisable material, the controller controls the current switching of the electromagnet, to grab the luminous micropore
Plate.
Further, further includes:
Reagent area, the reagent area are provided with for putting auxiliary reagents, the reagent areas such as prevention macromolecular agent
Sensor, for incuding the reagent magnitude of the reagent area；
Sample needle area, for placing disposable sample needle, the end of the sample arm, which is provided with, to be used in the sample needle area
The feed head of disposable sample needle is installed.
Further, further includes: temperature detector, the temperature detector is for detecting the first couveuse and the second incubation
The incubation temperature of device.
The course of work at the full-automatic nucleic acid signal amplification detection automatic work station is as follows:
S1, sample arm grab the luminous microwell plate being loaded, and place it in the first couveuse pallet, in the temperature of setting
A period of time t1 is incubated under T1；
S2, it is incubated for and terminates in the first couveuse, luminous microwell plate is transferred to board-washing from the first couveuse by sample arm
On machine, board-washing operation is carried out；
After S3, board-washing, the board-washing microwell plate that shines is transferred to the position of the second couveuse by sample arm from board-washing machine
On, then configured pre-amplification molecular agents are added into luminous microwell plate for sample arm；
S4, prevent addition in the second couveuse the luminous microwell plate of macromolecular agent in setting temperature identical with step S1
It spends and is incubated for a period of time t2 under T1；
After S5, completion step S4, luminous microwell plate is transferred on board-washing machine by sample arm from the second couveuse, is washed
Plate operation；
S6, after completing step S5, the board-washing microwell plate that shines is transferred to the position of the second couveuse by sample arm from board-washing machine
It sets, then configured amplification molecule reagent is added into luminous microwell plate for sample arm；
S7, prevent addition in the second couveuse the luminous microwell plate of macromolecular agent in setting temperature identical with step S1
It spends and is incubated for a period of time t2 under T1；
After S8, completion step S7, luminous microwell plate is transferred on board-washing machine by sample arm from the second couveuse, is washed
Plate operation；
S9, after completing step S8, the board-washing microwell plate that shines is transferred to the position of the second couveuse by sample arm from board-washing machine
It sets, then configured probe marker reagent is added into luminous microwell plate for sample arm；
S10, one section is incubated at the temperature T2 of setting to the luminous microwell plate that probe marker reagent is added in step S9
Time t2；
S11, after completing step S10, step S5 is repeated；
S12, after completing step S11, the board-washing microwell plate that shines is transferred to the second couveuse by sample arm from board-washing machine
On position, then configured substrate mix reagent is added into luminous microwell plate for sample arm；
S13, in step S12 be added substrate mix reagent luminous microwell plate one section is incubated at the temperature T3 of setting when
Between t3；
S14, complete step S13 after, sample arm luminous microwell plate is transferred to from the second couveuse in interpretative instrument into
Row analysis；
S15, after completing step S14, that is, the detection to sample of nucleic acid is completed.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention by by working station by couveuse, board-washing machine, interpretoscope, sample arm, reagent, sample needle into
Row is integrated, and realizes automatic operating of sample arm during sample incubation by controller, starts building certainly to effectively improve
Make the integrated level and automation stood；
2, it is practical second according to the difference of addition after the present invention carries out place's incubation in the first couveuse to sample of nucleic acid
The multiple incubation of different temperature points and different time points is carried out in couveuse, to realize in same equipment under different temperature points
Sample of nucleic acid signal amplification detection；
3, the present invention realizes that sample of nucleic acid is being set by the mutual cooperation of controller, sample arm, sensor, temperature detector
Incubation operation orderly, smoothly is carried out in fixed program, effectively improves the work of nucleic acid signal amplification detection；
In short, the present invention has the advantages that easy to use, integrated height, detection efficiency are high, intelligentized.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention.
Wherein, 1, working station, the 2, first couveuse, the 3, second couveuse, 4, board-washing machine, 5, interpretoscope, 6, sample-adding
Arm, 7, couveuse lid, 8, reagent area, 9, sample needle area, 10, temperature detector, 11, quality control region.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Embodiment 1
A kind of full-automatic nucleic acid signal amplification detection automatic work station, including working station 1, the working station 1
Inside it is provided with
First couveuse 2, first couveuse 2 are used to carry out hybridization incubation to sample of nucleic acid；
Second couveuse 3, second couveuse 3 are used to carry out calculating to sample of nucleic acid signal amplification and are incubated for；
Board-washing machine 4, the board-washing machine 4 are used to carry out board-washing operation to by the sample of nucleic acid of hybridization incubation；
Interpretoscope 5, the interpretoscope 5 are used to analyze by calculating the sample of nucleic acid that signal amplification is incubated for；
Sample arm 6, the sample arm 6 is for realizing sample of nucleic acid in the first couveuse 2, the second couveuse 3, board-washing machine 4
And the moving operation between interpretoscope 5；
It further include controller, the controller moves sample of nucleic acid according to the program of setting for controlling sample arm 6
Dynamic, for controlling the first couveuse 2, the second couveuse 3 is incubated for sample of nucleic acid, for controlling board-washing machine 4 to nucleic acid sample
Product carry out board-washing, test and analyze for controlling interpretoscope 5 to sample of nucleic acid.
In the present embodiment, since the size of interpretoscope is higher, in order to save the space of work station, the working station 1
Be provided with the groove for placing interpretoscope 5, interpretoscope 5 at least 3/5 at be located in groove.
In the present embodiment, the couveuse lid 7 being used cooperatively with the second couveuse 3 is placed on the working station 1.
In the present embodiment, the sample arm 6 includes: sample-adding arm body, and the sample-adding arm body is equipped with lateral displacement device,
Length travel device, telescopic displacement device, for carrying out all directions to 6 arm body of sample arm under the control of the controller
Displacement.
In the present embodiment, the sample-adding arm body is connect with the working station by rotary electric machine, the sample arm 6
Whole inverted V shaped structure, the angle of the inverted V-shaped structure can change under the control of the controller.
In the present embodiment, the end of the sample arm 6 is equipped with clamping part, for clamping the luminous micropore for placing sample of nucleic acid
Plate.
In the present embodiment, the end of the sample arm 6 is equipped with electromagnet, correspondingly, for placing shining for sample of nucleic acid
The corresponding position of microwell plate is equipped with magnetisable material, and the controller controls the current switching of the electromagnet, to grab the hair
Light microwell plate.
In the present embodiment, further includes:
Reagent area 8, the reagent area 8 are arranged for putting the auxiliary reagents such as prevention macromolecular agent, the reagent area 8
There is sensor, for incuding the reagent magnitude of the reagent area 8；
Sample needle area 9, for placing disposable sample needle, the end setting of the sample arm 6 is useful in the sample needle area 9
In the feed head for installing disposable sample needle；
And quality control region 11, the quality control region 11 is for controlling the accurate of the amplified experimental result data of nucleic acid signal
Property.
In the present embodiment, further includes: temperature detector 10, the temperature detector 10 for detect the first couveuse 2 with
And second couveuse 3 incubation temperature, and the temperature feedback that will test of temperature detector 10 is to controller.
In order to avoid shining, the sample of nucleic acid in microwell plate evaporates during incubation with heat, in the upper of sample of nucleic acid solution
Side is sealed using sealing paraffin wax oil.
Embodiment 2
The present embodiment is the full-automatic nucleic acid signal amplification detection automatic work station that is provided based on embodiment 1 to sample of nucleic acid
Calculate the process of signal amplification detection.
The course of work at the full-automatic nucleic acid signal amplification detection automatic work station is as follows:
S1, sample arm 6 grab the luminous microwell plate being loaded, and place it in 2 pallet of the first couveuse, in the temperature of setting
It spends and is incubated for a period of time t1 under T1；
S2, it is incubated for and terminates in the first couveuse 2, luminous microwell plate is transferred to from the first couveuse 2 and washes by sample arm 6
On trigger 4, board-washing operation is carried out；
After S3, board-washing, the board-washing microwell plate that shines is transferred to the position of the second couveuse 3 by sample arm 6 from board-washing machine 4
It sets, then configured pre-amplification molecular agents are added into luminous microwell plate for sample arm；
S4, the luminous microwell plate of macromolecular agent is prevented in setting identical with step S1 to addition in the second couveuse 3
A period of time t2 is incubated under temperature T1；
After S5, completion step S4, luminous microwell plate is transferred on board-washing machine 4 by sample arm 6 from the second couveuse 3, into
The operation of row board-washing；
S6, after completing step S5, the board-washing microwell plate that shines is transferred to the second couveuse 3 by sample arm 6 from board-washing machine 4
On position, then configured amplification molecule reagent is added into luminous microwell plate for sample arm；
S7, the luminous microwell plate of macromolecular agent is prevented in setting identical with step S1 to addition in the second couveuse 3
A period of time t2 is incubated under temperature T1；
After S8, completion step S7, luminous microwell plate is transferred on board-washing machine 4 by sample arm 6 from the second couveuse 3, into
The operation of row board-washing；
S9, after completing step S8, the board-washing microwell plate that shines is transferred to the second couveuse 3 by sample arm 6 from board-washing machine 4
On position, then configured probe marker reagent is added into luminous microwell plate for sample arm；
S10, one section is incubated at the temperature T2 of setting to the luminous microwell plate that probe marker reagent is added in step S9
Time t2；
S11, after completing step S10, step s5 is repeated；
S12, after completing step S11, the board-washing microwell plate that shines is transferred to the second couveuse 3 by sample arm 6 from board-washing machine 4
Position on, then configured substrate mix reagent is added into luminous microwell plate for sample arm；
S13, in step S12 be added substrate mix reagent luminous microwell plate one section is incubated at the temperature T3 of setting when
Between t3:
After S14, completion step S13, luminous microwell plate is transferred in interpretative instrument by sample arm 6 from the second couveuse 3
It is analyzed；
S15, after completing step S14, that is, the detection to sample of nucleic acid is completed.
In the present embodiment, preferably, the temperature T1 is 55 DEG C, the time t1 is 3.5h, and the temperature T2 is
50 DEG C, the time t2 is 40min, and the temperature T2 is 46 DEG C, and the time t3 is 20min.
When carrying out the present embodiment:
Specifically the program of the temperature (T1, T2, T3) of the incubation of sample of nucleic acid, time (t1, t2, t3) and the course of work is equal
Carry out input setting by controller, and by controller to the first couveuse 2, the second couveuse 3, board-washing machine 4, interpretoscope 5,
Sample arm 6 is controlled by the operation program of setting, during carrying out every operation, when the first couveuse 2 is incubated with second
T1, T2, T3 that 3 temperature of device reaches setting are educated, incubation time reaches t1, t2, t3 of setting, and controller will control the first couveuse
2 and second couveuse 3 stop heating and it is out of service；
And controller controls sample arm 6 according to the needs for carrying out nucleic acid signal amplification incubation process to sample of nucleic acid
According to different accounting signal amplification incubation processes reagent area 8 draw suitable prevention macromolecular agent or amplification molecule reagent or
Probe marker reagent or substrate mix reagent are added in the sample of nucleic acid on luminous microwell plate；
Before sample arm 6 draws corresponding reagent in reagent area 8, controller controls sample arm 6 and carries out in sample needle area 9
The replacement of disposable sample needle, i.e., sample arm 6 is moved to reagent area 8 and draws corresponding reagent after to the replacement of disposable sample needle
It is added in the nucleic acid samples of luminous microwell plate, sensor sense during carrying out reagent absorption each time, in reagent area 8
The amount that reagent should be drawn every time, after reaching the amount of reagent of setting, sample arm 6 stops drawing corresponding reagent；
When using the second couveuse 3 to carry out nucleic acid signal amplification each time, the second couveuse 3 is in closed state,
That is 7 hamper of couveuse lid is on the second couveuse 3, and sample arm 6 is both needed to remove on couveuse lid 7 after being incubated for every time, removes
Afterwards, it is same to carry out board-washing operation, sample arm 6 is by 7 hamper of couveuse lid in the second couveuse 3 after each filling for completing reagent
On, the second couveuse 3 begins to warm up incubation after hamper.
For the ease of sample arm 6 in the first couveuse 2, the second couveuse 3, board-washing machine 4, interpretoscope 5, couveuse lid 7, examination
Accurate running fix between agent area 8, sample needle area 9, have on working station 1 with the first couveuse 2, the second couveuse 3,
Board-washing machine 4, interpretoscope 5, couveuse lid 7, reagent area 8, the corresponding preset areas in sample needle area 9, sample arm 6 according to it is above-mentioned it is complete from
The course of work at kinetonucleus acid signal amplification detection automatic work station orderly, accurately moves.
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art,
It is still possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is carried out etc.
With replacement, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this
Within the protection scope of invention.
Claims (10)
1. a kind of full-automatic nucleic acid signal amplification detection automatic work station, which is characterized in that described including working station (1)
It is provided in working station (1)
First couveuse (2), first couveuse (2) are used to carry out hybridization incubation to sample of nucleic acid；
Second couveuse (3), second couveuse (3) are used to carry out calculating to sample of nucleic acid signal amplification and are incubated for；
Board-washing machine (4), the board-washing machine (4) are used to carry out board-washing operation to by the sample of nucleic acid of hybridization incubation；
Interpretoscope (5), the interpretoscope (5) are used to analyze by calculating the sample of nucleic acid that signal amplification is incubated for；
Sample arm (6), the sample arm (6) is for realizing sample of nucleic acid in the first couveuse (2), the second couveuse (3), board-washing
Moving operation between machine (4) and interpretoscope (5)；
It further include controller, the controller moves sample of nucleic acid according to the program of setting for controlling sample arm (6),
For controlling the first couveuse (2), the second couveuse (3) is incubated for sample of nucleic acid, for controlling board-washing machine (4) to nucleic acid
Sample carries out board-washing, tests and analyzes for controlling interpretoscope (5) to sample of nucleic acid.
2. full-automatic nucleic acid signal amplification detection automatic work station as described in claim 1, it is characterised in that: the work station
Ontology (1) is provided with the groove for placing interpretoscope (5).
3. full-automatic nucleic acid signal amplification detection automatic work station as described in claim 1, it is characterised in that: the work station
The couveuse lid (7) being used cooperatively with the second couveuse (3) is placed on ontology (1).
4. full-automatic nucleic acid signal amplification detection automatic work station as described in claim 1, which is characterized in that the sample arm
(6) include: sample-adding arm body, the sample-adding arm body be equipped with lateral displacement device, length travel device, telescopic displacement device,
For the displacement of all directions to be carried out to sample arm (6) arm body under the control of the controller.
5. full-automatic nucleic acid signal amplification detection automatic work station as claimed in claim 4, it is characterised in that: the sample arm
Ontology is connect with the working station by rotary electric machine, the whole inverted V shaped structure of the sample arm (6), the inverted V-shaped knot
The angle of structure can change under the control of the controller.
6. full-automatic nucleic acid signal amplification detection automatic work station as described in claim 1, it is characterised in that: the sample arm
(6) end is equipped with clamping part, for clamping the luminous microwell plate for placing sample of nucleic acid.
7. full-automatic nucleic acid signal amplification detection automatic work station as described in claim 1, it is characterised in that: the sample arm
(6) end is equipped with electromagnet, correspondingly, is equipped with magnetic material for placing the corresponding position of luminous microwell plate of sample of nucleic acid
Matter, the controller control the current switching of the electromagnet, to grab the luminous microwell plate.
8. such as the described in any item full-automatic nucleic acid signal amplification detection automatic work stations claim 1-7, which is characterized in that also
Include:
Reagent area (8), the reagent area (8) set for putting auxiliary reagents, the reagent areas (8) such as prevention macromolecular agent
It is equipped with sensor, for incuding the reagent magnitude of the reagent area (8)；
Sample needle area (9), for placing disposable sample needle, the end of the sample arm (6) is provided with the sample needle area (9)
For installing the feed head of disposable sample needle.
9. full-automatic nucleic acid signal amplification detection automatic work station as claimed in claim 8, which is characterized in that further include: temperature
It spends detector (10), the temperature detector (10) is used to detect the incubation temperature of the first couveuse (2) and the second couveuse (3)
Degree.
10. full-automatic nucleic acid signal amplification detection automatic work station as claimed in claim 9, which is characterized in that its is worked
Journey is as follows:
S1, sample arm (6) grab the luminous microwell plate being loaded, and place it in the first couveuse (2) pallet, in the temperature of setting
It spends and is incubated for a period of time t1 under T1；
S2, incubation terminates in the first couveuse (2), and luminous microwell plate is transferred to by sample arm (6) from the first couveuse (2)
On board-washing machine (4), board-washing operation is carried out；
After S3, board-washing, the board-washing microwell plate that shines is transferred to the second couveuse (3) by sample arm (6) from board-washing machine (4)
On position, then configured pre-amplification molecular agents are added into luminous microwell plate for sample arm；
S4, prevent addition in the second couveuse (3) the luminous microwell plate of macromolecular agent in setting temperature identical with step S1
It spends and is incubated for a period of time t2 under T1；
After S5, completion step S4, luminous microwell plate is transferred on board-washing machine (4) by sample arm (6) from the second couveuse (3),
Carry out board-washing operation；
S6, after completing step S5, the board-washing microwell plate that shines is transferred to the second couveuse (3) by sample arm (6) from board-washing machine (4)
Position on, then configured amplification molecule reagent is added into luminous microwell plate for sample arm；
S7, prevent addition in the second couveuse (3) the luminous microwell plate of macromolecular agent in setting temperature identical with step S1
It spends and is incubated for a period of time t2 under T1；
After S8, completion step S7, luminous microwell plate is transferred on board-washing machine (4) by sample arm (6) from the second couveuse (3),
Carry out board-washing operation；
S9, after completing step S8, the board-washing microwell plate that shines is transferred to the second couveuse (3) by sample arm (6) from board-washing machine (4)
Position on, then configured probe marker reagent is added into luminous microwell plate for sample arm；
S10, is incubated at the temperature T2 of setting to the luminous microwell plate that probe marker reagent is added in step S9 a period of time
t2；
S11, after completing step S10, step S5 is repeated；
S12, after completing step S11, the board-washing microwell plate that shines is transferred to the second couveuse from board-washing machine (4) by sample arm (6)
(3) on position, then configured substrate mix reagent is added into luminous microwell plate for sample arm；
S13, is incubated at the temperature T3 of setting to the luminous microwell plate that substrate mix reagent is added in step S12 a period of time
T3:
After S14, completion step S13, luminous microwell plate is transferred in interpretative instrument by sample arm (6) from the second couveuse (3)
It is analyzed；
S15, after completing step S14, that is, the detection to sample of nucleic acid is completed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811523158.1A CN109456886A (en) | 2018-12-12 | 2018-12-12 | A kind of full-automatic nucleic acid signal amplification detection automatic work station |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811523158.1A CN109456886A (en) | 2018-12-12 | 2018-12-12 | A kind of full-automatic nucleic acid signal amplification detection automatic work station |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109456886A true CN109456886A (en) | 2019-03-12 |
Family
ID=65613319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811523158.1A Pending CN109456886A (en) | 2018-12-12 | 2018-12-12 | A kind of full-automatic nucleic acid signal amplification detection automatic work station |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109456886A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112831599A (en) * | 2020-12-07 | 2021-05-25 | 郑州科蒂亚生物技术有限公司 | CoVID-19 virus detection kit based on signal amplification technology |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030032029A1 (en) * | 1998-12-21 | 2003-02-13 | Nanogen, Inc. | Three dimensional apparatus and method for integrating sample preparation and multiplex assays |
| CN101387650A (en) * | 2007-12-20 | 2009-03-18 | 北京科美生物技术有限公司 | Full automatic plate type luminous instrument and luminous detecting method thereof |
| CN103674935A (en) * | 2013-12-05 | 2014-03-26 | 青岛科技大学 | Method for determining gibberellin based on hybridization chain-reaction signal amplification technology |
| CN103695551A (en) * | 2013-12-24 | 2014-04-02 | 华南师范大学 | Nucleic acid detection method based on polymer electrochemiluminescence signal amplification technology |
| WO2015127656A1 (en) * | 2014-02-28 | 2015-09-03 | 深圳市第二人民医院 | Human immunodeficiency virus testing device and testing method thereof |
| CN106970231A (en) * | 2017-03-31 | 2017-07-21 | 中国药科大学 | The high-sensibility chemical luminescence immunoassay method of Nano silver grain is marked based on analogue enztme |
| CN107831322A (en) * | 2017-12-08 | 2018-03-23 | 郑州科蒂亚生物技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter device |
| CN108796134A (en) * | 2018-07-12 | 2018-11-13 | 郑州科蒂亚生物技术有限公司 | A kind of cervical carcinoma detection probe sequence and its application |
-
2018
- 2018-12-12 CN CN201811523158.1A patent/CN109456886A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030032029A1 (en) * | 1998-12-21 | 2003-02-13 | Nanogen, Inc. | Three dimensional apparatus and method for integrating sample preparation and multiplex assays |
| CN101387650A (en) * | 2007-12-20 | 2009-03-18 | 北京科美生物技术有限公司 | Full automatic plate type luminous instrument and luminous detecting method thereof |
| CN103674935A (en) * | 2013-12-05 | 2014-03-26 | 青岛科技大学 | Method for determining gibberellin based on hybridization chain-reaction signal amplification technology |
| CN103695551A (en) * | 2013-12-24 | 2014-04-02 | 华南师范大学 | Nucleic acid detection method based on polymer electrochemiluminescence signal amplification technology |
| WO2015127656A1 (en) * | 2014-02-28 | 2015-09-03 | 深圳市第二人民医院 | Human immunodeficiency virus testing device and testing method thereof |
| CN106970231A (en) * | 2017-03-31 | 2017-07-21 | 中国药科大学 | The high-sensibility chemical luminescence immunoassay method of Nano silver grain is marked based on analogue enztme |
| CN107831322A (en) * | 2017-12-08 | 2018-03-23 | 郑州科蒂亚生物技术有限公司 | A kind of Full-automatic chemiluminescence immunoassay analysis meter device |
| CN108796134A (en) * | 2018-07-12 | 2018-11-13 | 郑州科蒂亚生物技术有限公司 | A kind of cervical carcinoma detection probe sequence and its application |
Non-Patent Citations (2)
| Title |
|---|
| MARK A. BAUMEISTER ET AL.: "A Sensitive Branched DNA HIV-1 Signal Amplification Viral Load Assay with Single Day Turnaround", 《PLOS ONE》 * |
| 莫志宏 等: "核酸信号放大检测及纳米技术的应用", 《中国卫生检验杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112831599A (en) * | 2020-12-07 | 2021-05-25 | 郑州科蒂亚生物技术有限公司 | CoVID-19 virus detection kit based on signal amplification technology |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4272351B2 (en) | Automatic execution device for chemical or biological methods | |
| US5436129A (en) | Process for specimen handling for analysis of nucleic acids | |
| AU2009201529B2 (en) | Apparatus For Polynucleotide Detection and Quantitation | |
| ATE118824T1 (en) | IMMOBILIZED OLIGONUCLEOTIDE PROBE AND USES THEREOF. | |
| US20220325272A1 (en) | Sample preparation apparatus and multi-well plate with pcr chip | |
| CN102660458B (en) | Nucleic acid extraction device capable of preventing cross-contamination | |
| DE3578349D1 (en) | HYBRIDIZATION TEST FOR NUCLEIC ACID USING DETECTABLE ANTI-HYBRID ANTIBODIES. | |
| ATE147792T1 (en) | NEW METHOD FOR DETECTING A SPECIFIC NUCLEIC ACID SEQUENCE IN A CELL SAMPLE | |
| CN105420086A (en) | Single-cell positioning microporous membrane, application and single-cell automatic acquisition device | |
| CN103290487A (en) | Chip of biological reaction device | |
| JP2010538672A (en) | Method, apparatus and molecular biology kit for extracting amplified genetic material | |
| CN109456886A (en) | A kind of full-automatic nucleic acid signal amplification detection automatic work station | |
| JP6374967B2 (en) | Detection of nucleic acid amplification in porous substrates | |
| Paba et al. | Performance evaluation of the Artus hepatitis C virus QS-RGQ assay | |
| WO2023155403A1 (en) | Nucleic acid test kit and test method | |
| CN208109771U (en) | A kind of laboratory COD automatically analyzes robot | |
| CN112921120B (en) | Reusable nucleic acid fluorescent probe and corresponding nucleic acid detection method | |
| CN110699438A (en) | Nucleic acid detection device and detection method | |
| CN205368351U (en) | Single cellular localization microporous membrane and unicellular automatic acquisition device | |
| CN109280663B (en) | Full-automatic nucleic acid single-strand preparation method | |
| CN110714054B (en) | Transgene detection method and kit based on electric field induced release detection technology combined with target gene fragment | |
| CN216738348U (en) | Nucleic acid multi-joint detection device suitable for isothermal amplification technology | |
| JP2013230146A (en) | Positive control concept | |
| CN108728579A (en) | A method of using the direct RT-PCR detections various plants virus of plant micro-assembly robot | |
| CN212340790U (en) | Automatic device for separating and detecting biomacromolecules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |