CN109452494A - A kind of Cynoglossus semilaevis feed addictive and preparation method thereof and application method - Google Patents
A kind of Cynoglossus semilaevis feed addictive and preparation method thereof and application method Download PDFInfo
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- CN109452494A CN109452494A CN201811167082.3A CN201811167082A CN109452494A CN 109452494 A CN109452494 A CN 109452494A CN 201811167082 A CN201811167082 A CN 201811167082A CN 109452494 A CN109452494 A CN 109452494A
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- 241001014350 Cynoglossus semilaevis Species 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 8
- 239000000843 powder Substances 0.000 claims abstract description 43
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 27
- 241000233671 Schizochytrium Species 0.000 claims abstract description 26
- 241000223252 Rhodotorula Species 0.000 claims abstract description 13
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 13
- 241000223253 Rhodotorula glutinis Species 0.000 claims abstract description 12
- 239000004615 ingredient Substances 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 239000000470 constituent Substances 0.000 claims abstract description 3
- 210000002421 cell wall Anatomy 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 230000037396 body weight Effects 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 5
- 230000003116 impacting effect Effects 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 235000021401 pellet diet Nutrition 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 claims 1
- 238000013467 fragmentation Methods 0.000 claims 1
- 238000006062 fragmentation reaction Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 11
- 230000036039 immunity Effects 0.000 abstract description 9
- 244000144972 livestock Species 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 7
- 230000003834 intracellular effect Effects 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 5
- 235000019786 weight gain Nutrition 0.000 description 4
- 102000016938 Catalase Human genes 0.000 description 3
- 108010053835 Catalase Proteins 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 1
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 1
- 206010001557 Albinism Diseases 0.000 description 1
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 1
- -1 Bu Su Substances 0.000 description 1
- 241001609213 Carassius carassius Species 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 241001417984 Cynoglossidae Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 241000237509 Patinopecten sp. Species 0.000 description 1
- 241000269978 Pleuronectiformes Species 0.000 description 1
- 241000157468 Reinhardtius hippoglossoides Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 244000111146 Sonchus arvensis Species 0.000 description 1
- 235000008135 Sonchus arvensis ssp. arvensis Nutrition 0.000 description 1
- 235000008132 Sonchus arvensis ssp. uliginosus Nutrition 0.000 description 1
- 241001595453 Symphurus nigrescens Species 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 235000013793 astaxanthin Nutrition 0.000 description 1
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 1
- 229940022405 astaxanthin Drugs 0.000 description 1
- 239000001168 astaxanthin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 1
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a kind of Cynoglossus semilaevis feed addictive, constituent and parts by weight are as follows: 50~70 parts of chlorella powder, 10~15 parts of ocean rhodotorula glutinis powder, 20~30 parts of schizochytrium powder, 0.01~0.05 part of chitosan oligosaccharide；The sum of parts by weight of composition described above ingredient are 100 parts.Feed addictive of the present invention, which has, promotes Cynoglossus semilaevis growth, improve Cynoglossus semilaevis non-specific immunity and survival rate, enhance the effect of premunition, chlorella powder, ocean rhodotorula glutinis powder, schizochytrium powder in the additive are broken wall powder, and intracellular nutritional ingredient is easier to be digested and assimilated by aquatic livestock；The intracellular nutritional ingredient of chlorella, ocean rhodotorula, schizochytrium complements one another, and adds with after the chitosan oligosaccharide for improving archenteric flora structure, feed addictive of the present invention shows stronger synergistic effect to the growth of aquatic livestock and immunity.
Description
Technical field
The invention belongs to aquatic feeds technical field, especially a kind of Cynoglossus semilaevis feed addictive and preparation method thereof and
Application method.
Background technique
Cynoglossus semilaevis belongs to Pleuronectiformes, Cynoglossidae, and tongue sole category is commonly called as field sowthistle herb, sole mesh, sole rice, equal in China Coast sea area
It is distributed；Cynoglossus semilaevis individual is big, growth is fast, meat flavour is delicious, and economic value is very high, is the rare seawater fish of China, in recent years
It is increasing to carry out its intensive culture scale.With the raising of Cynoglossus semilaevis intensive culture scale, disease problem is increasingly tight
Weight.Exploitation can promote Cynoglossus semilaevis to grow and the functional feedstuff additive of strengthen immunity and premunition, double of sliding-tongue
Sole healthy aquaculture industry has important impetus.
Chlorella is rich in protein and somatomedin, substitutes the fish meal of 47.14-49.88% in feed, can improve crucian carp
Rate of body weight gain reduces feed coefficient；Chlorella also promotes Growth of Litopenaeus vannamei, improves its immunity.
Ocean rhodotorula cell is rich in protein, unsaturated fatty acid, essential amino acid, and metabolite contains class Hu trailing plants
The various actives substance such as Bu Su, vitamin, astaxanthin is usually used in as the fresh and alive of aquatic livestock juvenile stage in culture fishery
Bait can promote aquatic animal growth as feed addictive, improve immunity.
Lipid content accounts for 50% of dry cell weight or more in schizochytrium powder, and docosahexaenoic acid (DHA) content is up to
20% or more.Schizochytrium powder both can directly feed the seedling such as scallop, oyster, be alternatively arranged as the nutrition addition of aquatic animal feed
Agent is remarkably improved survival rate, the anti-stress ability of fry, moreover it is possible to effectively prevent young turbot " albinism ".
Chitosan oligosaccharide is the monomer glycan after chitosan is digested.Chitosan oligosaccharide is applied to animal husbandry and fishery animal, can improve digestion
Road flora improves immunity of organisms, can be used for disease prevention and cure.
In the document delivered, the cell wall of chlorella, ocean rhodotorula and schizochytrium is not crushed, intact cell
The presence of wall makes chlorella, ocean rhodotorula and schizochytrium be difficult to be digested completely by aquatic livestock, intracellular nutrients
Matter is more difficult to be fully absorbed by aquatic livestock；In addition, chlorella, ocean rhodotorula, schizochytrium and chitosan oligosaccharide are individually to add
It is added in aquatic animal feed, it may be not as good as the effect after compounding to the beneficial effect of aquatic livestock.
By retrieval, patent publication us relevant to present patent application is not yet found.
Summary of the invention
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of Cynoglossus semilaevis feed addictive and its
Preparation method and application method, which, which has, promotes Cynoglossus semilaevis growth, improves Cynoglossus semilaevis non-specific immunity
And survival rate, enhance the effect of premunition.
The present invention solves its technical problem and adopts the following technical solutions to achieve:
A kind of Cynoglossus semilaevis feed addictive, constituent and parts by weight are as follows:
50~70 parts of chlorella powder after broken wall crushing, 10~15 parts of ocean rhodotorula glutinis powder after broken wall crushing, after broken wall crushes
20~30 parts of schizochytrium powder, 0.01~0.05 part of chitosan oligosaccharide；The sum of parts by weight of composition described above ingredient are 100 parts.
A kind of preparation method of Cynoglossus semilaevis feed addictive as described above, steps are as follows:
After the weighing of each raw material, crushed after mixing, to get Cynoglossus semilaevis feed addictive after sieving with 100 mesh sieve.
Moreover, schizochytrium after ocean rhodotorula glutinis powder, broken wall crush after chlorella powder, broken wall crush after the broken wall crushing
Powder be respectively will chlorella, ocean rhodotorula, schizochytrium broken wall crush after obtain, that is, require by chlorella, ocean rhodotorula,
The cell wall of schizochytrium is broken.
Moreover, chlorella powder is to utilize high pressure homogenizer by the chlorella concentrate after heterotrophic fermentation after the broken wall crushes
Break its cell wall, then spray-dried acquisition through impacting with high pressure；
Schizochytrium powder is to utilize high pressure homogenizer by the schizochytrium concentrate after heterotrophic fermentation after the broken wall crushes
Break its cell wall, then spray-dried acquisition through impacting with high pressure；
Ocean rhodotorula glutinis powder is by the ocean rhodotorula of heterotrophic fermentation through high pressure homogenizer broken wall, spraying after the broken wall crushes
It is dry to obtain.
The application method of Cynoglossus semilaevis feed addictive as described above, steps are as follows:
It is by mass percentage 5 ‰~15 ‰ to be added to Cynoglossus semilaevis artifical compound feed by Cynoglossus semilaevis feed addictive
In, sinking pellet diet is made, continuous feeding Cynoglossus semilaevis 30 days or more, day feeding volume accounted for the 1 ± 0.5% of fish body weight, daily
Divide 2-3 times and feeds.
The advantages of present invention obtains and good effect are:
1, the chlorella powder in feed addictive of the present invention, ocean rhodotorula glutinis powder, schizochytrium powder are broken wall powder, carefully
Nutritional ingredient intracellular is easier to be digested and assimilated by aquatic livestock；The intracellular nutrition of chlorella, ocean rhodotorula, schizochytrium at
Divide and complement one another, adds with after the chitosan oligosaccharide for improving archenteric flora structure, feed addictive of the present invention is to aquatic livestock
Growth and immunity show stronger synergistic effect.
2, feed addictive of the present invention can promote the growth of Cynoglossus semilaevis, improve Cynoglossus semilaevis non-specific immunity
And survival rate, enhance the disease resistance of Cynoglossus semilaevis.All raw materials of the feed addictive are the natural material of green, health,
It has no toxic side effect to Cynoglossus semilaevis, it is pollution-free to water environment.
Specific embodiment
Below with reference to the invention will be further described by specific embodiment, following embodiment be it is descriptive, no
It is restrictive, this does not limit the scope of protection of the present invention.
Raw material used in the present invention is unless otherwise specified conventional commercial product；Used in the present invention
Method is unless otherwise specified the conventional method of this field.
The raw materials used in the present invention breaking wall of chlorella powder can use high pressure homogenizer and the chlorella after heterotrophic fermentation be concentrated
Liquid breaks its cell wall, then spray-dried acquisition through impacting with high pressure.Schizochytrium powder can use high pressure homogenizer for heterotrophism
Schizochytrium concentrate after fermentation breaks its cell wall, then spray-dried acquisition through impacting with high pressure.Chitosan oligosaccharide is gathered by shell
Carbohydrase solution into (preparation method of chitosan oligosaccharide uses customary preparation methods in the art), sent out by heterotrophism by ocean rhodotorula glutinis powder
The ocean rhodotorula of ferment is obtained through high pressure homogenizer broken wall, spray drying.
Embodiment 1:
A kind of Cynoglossus semilaevis feed addictive, the feed addictive include following composition by weight: chlorella powder 55%, ocean
Rhodotorula glutinis powder 15%, schizochytrium powder 29.95%, chitosan oligosaccharide 0.05%；The above raw material is weighed, crush after mixing, crosses 100 mesh
It after sieve, is added in Cynoglossus semilaevis feed according to 5 ‰ ratio, the continuous initial weight of feeding is the Cynoglossus semilaevis 60 of 150g ± 5g
Found after it, compared with the control group for not adding the feed addictive, 5 ‰ groups of Cynoglossus semilaevis survival rate of addition improve to
98.7% or more, the death rate is reduced to 1.8% or less.Rate of body weight gain improves 8.79%, and specific growth rate improves 18.57%, blood plasma
Middle total number born activity improves 5.50%, and total antioxidant capacity improves 13.16%, and catalase activity improves
204.58%, mda content reduces by 30.42%, specifically see the table below.
Embodiment 2:
A kind of Cynoglossus semilaevis feed addictive, the feed addictive include following composition by weight: chlorella powder 60%, ocean
Rhodotorula glutinis powder 13%, schizochytrium powder 26.99%, chitosan oligosaccharide 0.01%；The above raw material is weighed, crush after mixing, crosses 100 mesh
After sieve, it is added in Cynoglossus semilaevis feed according to 10 ‰ ratio.The Cynoglossus semilaevis that the continuous initial weight of feeding is 230g ± 10g
It is found after 60 days, compared with the control group, survival rate is improved to 98.8% or more, and the death rate is reduced to 1.2% or less.Rate of body weight gain mentions
High by 7.42%, specific growth rate improves 23.43%, and total number born activity improves 13.51% in blood plasma, total antioxidation
Ability improves 53.15%, and catalase activity improves 369.44%, and mda content reduces by 36.57%, specifically see the table below.
Embodiment 3:
A kind of Cynoglossus semilaevis feed addictive, the feed include following composition by weight: chlorella powder 65%, ocean rhodotorula
Powder 10%, schizochytrium powder 24.97%, chitosan oligosaccharide 0.03%；The above raw material is weighed, crush, sieve with 100 mesh sieve after mixing after,
It is added in Cynoglossus semilaevis feed according to 15 ‰ ratio.The continuous initial weight of feeding is Cynoglossus semilaevis 60 days of 327g ± 50g
After find, compared with the control, survival rate is improved to 98.3% or more, and the death rate is reduced to 2.0% or less.Rate of body weight gain improves
10.35%, specific growth rate improves 17.81%, and total number born activity improves 6.44% in blood plasma, total antioxidation energy
Power improves 28.17%, and catalase activity improves 96.52%, and mda content reduces by 26.76%, specifically see the table below.
Although disclosing the embodiment of the present invention for the purpose of illustration, it will be appreciated by those skilled in the art that: not
Be detached from the present invention and spirit and scope of the appended claims in, various substitutions, changes and modifications be all it is possible, therefore, this
The range of invention is not limited to the embodiment disclosure of that.
Claims (5)
1. a kind of Cynoglossus semilaevis feed addictive, it is characterised in that: its constituent and parts by weight are as follows:
50~70 parts of chlorella powder after broken wall crushing, 10~15 parts of ocean rhodotorula glutinis powder after broken wall crushing, fragmentation after broken wall crushes
20~30 parts of pot algae powder, 0.01~0.05 part of chitosan oligosaccharide；The sum of parts by weight of composition described above ingredient are 100 parts.
2. a kind of preparation method of Cynoglossus semilaevis feed addictive as described in claim 1, it is characterised in that: steps are as follows:
After the weighing of each raw material, crushed after mixing, to get Cynoglossus semilaevis feed addictive after sieving with 100 mesh sieve.
3. the preparation method of Cynoglossus semilaevis feed addictive according to claim 2, it is characterised in that: the broken wall crushes
Schizochytrium powder is by chlorella, the red ferment in ocean respectively after ocean rhodotorula glutinis powder, broken wall crushing after chlorella powder, broken wall crush afterwards
Female, schizochytrium broken wall obtains after crushing, that is, requires for the cell wall of chlorella, ocean rhodotorula, schizochytrium to be crushed.
4. the preparation method of Cynoglossus semilaevis feed addictive according to claim 3, it is characterised in that: the broken wall crushes
Afterwards chlorella powder be the chlorella concentrate after heterotrophic fermentation is broken into its cell wall through impacting with high pressure using high pressure homogenizer, then
Spray-dried acquisition；
Schizochytrium powder is by the schizochytrium concentrate after heterotrophic fermentation using high pressure homogenizer through height after the broken wall crushes
Its cell wall, then spray-dried acquisition are broken in pressure impact；
Ocean rhodotorula glutinis powder is by the ocean rhodotorula of heterotrophic fermentation through high pressure homogenizer broken wall, spray drying after the broken wall crushes
It obtains.
5. the application method of Cynoglossus semilaevis feed addictive as described in claim 1, it is characterised in that: steps are as follows:
It is by mass percentage 5 ‰~15 ‰ to be added in Cynoglossus semilaevis artifical compound feed by Cynoglossus semilaevis feed addictive,
Sinking pellet diet is made, continuous feeding Cynoglossus semilaevis 30 days or more, day feeding volume accounted for the 1 ± 0.5% of fish body weight, every bu 2-
It feeds for 3 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811167082.3A CN109452494A (en) | 2018-10-08 | 2018-10-08 | A kind of Cynoglossus semilaevis feed addictive and preparation method thereof and application method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811167082.3A CN109452494A (en) | 2018-10-08 | 2018-10-08 | A kind of Cynoglossus semilaevis feed addictive and preparation method thereof and application method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102132765A (en) * | 2011-03-01 | 2011-07-27 | 厦门汇盛生物有限公司 | Nutrition enhancer compounding schizochytrium aggregatum powder and phaffia rhodozyma powder, and application thereof on cultivation |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN104543528A (en) * | 2014-12-30 | 2015-04-29 | 青岛农业大学 | Cynoglossus semilaevis larva pellet feed and preparation method |
| CN106212854A (en) * | 2016-08-28 | 2016-12-14 | 北海生巴达生物科技有限公司 | A kind of feedstuff chlorella and probiotic bacteria mixed powder production method |
| CN108125071A (en) * | 2018-01-25 | 2018-06-08 | 青岛科海生物有限公司 | A kind of feed for litopenaeus vannamei additive and its application method |
-
2018
- 2018-10-08 CN CN201811167082.3A patent/CN109452494A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102132765A (en) * | 2011-03-01 | 2011-07-27 | 厦门汇盛生物有限公司 | Nutrition enhancer compounding schizochytrium aggregatum powder and phaffia rhodozyma powder, and application thereof on cultivation |
| CN104522425A (en) * | 2014-12-30 | 2015-04-22 | 青岛农业大学 | Granular feed for adult fish of cynoglossus semilaevis and preparation method of granular feed |
| CN104543528A (en) * | 2014-12-30 | 2015-04-29 | 青岛农业大学 | Cynoglossus semilaevis larva pellet feed and preparation method |
| CN106212854A (en) * | 2016-08-28 | 2016-12-14 | 北海生巴达生物科技有限公司 | A kind of feedstuff chlorella and probiotic bacteria mixed powder production method |
| CN108125071A (en) * | 2018-01-25 | 2018-06-08 | 青岛科海生物有限公司 | A kind of feed for litopenaeus vannamei additive and its application method |
Non-Patent Citations (3)
| Title |
|---|
| 田相利等: "《半滑舌鳎健康养殖实用新技术》", 31 January 2010, 海洋出版社 * |
| 赵书林等: "裂殖壶菌不同破壁方法的研究", 《中国油脂》 * |
| 钟韵山等: "小球藻破壁技术研究进展", 《食品研究与开发》 * |
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