CN109439690A - A kind of two-way solid fermentation process of monascus-Chinese medicine - Google Patents
A kind of two-way solid fermentation process of monascus-Chinese medicine Download PDFInfo
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- CN109439690A CN109439690A CN201811357192.6A CN201811357192A CN109439690A CN 109439690 A CN109439690 A CN 109439690A CN 201811357192 A CN201811357192 A CN 201811357192A CN 109439690 A CN109439690 A CN 109439690A
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Abstract
The invention belongs to Chinese traditional medicine biology technical fields, and in particular to a kind of two-way solid fermentation process of monascus-Chinese medicine.It comprises the steps of: (1) crushing the Chinese medicine of mass ratio 1:(1-3) and rice by pulverizer, then 20 meshes are crossed, again plus distilled water mixes after impregnating and obtains fermentation substrate, fermentation substrate is fitted into the culture bottle of 250mL, 2 hours of sterilizing at 115-120 DEG C are put into high-pressure sterilizing pot；(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in be protected from light in ventilated environment and is fermented；(3) tunning, 60 DEG C of drying are collected after fermentation time.The two-way solid fermentation process of monascus-Chinese medicine of the invention can improve active ingredients from traditional Chinese medicinal content, convert the new active constituent of generation, mitigate toxic side effect, have important social effect and economic value.
Description
Technical field
The invention belongs to Chinese traditional medicine biology technical fields, and in particular to a kind of two-way solid fermentation process of monascus-Chinese medicine.
Background technique
Monascus is China's traditional zymotic product --- the production strain of red yeast rice, monascus are inoculated in rice medium through sending out
Product after ferment is red yeast rice, has the applicating history of more than one thousand years in China, be mainly used for food color, preservative and in
Medicine compatibility.Bioconversion is that the various enzymes proposed using microorganism or from microorganism make substrate carry out certain as biocatalyst
Kind chemical reaction.
Chinese medicine active constituent is usually because content is low, resource is limited, structure is complicated, is unable to the disadvantages of chemical synthesis and difficult
To develop into new drug.Microorganism especially fungi has the ability of very strong decomposition and inversion substance, and can generate abundant secondary
Metabolite, therefore, with it is general either physically or chemically compared with, pharmacological property can be significantly changed using microorganism conversion Chinese herbal medicine,
Curative effect is improved, toxic side effect is reduced, expands indication.Professors Zhuan Yi etc. create " two-way solid fermentation " first and try out,
Medicinal fungi is seeded in Chinese medicine matrix, carries out solid fermentation under given conditions, generation has effects that new component, new
Medicinal fungal substance, since " pharmacological property matrix " can not only provide nutrition needed for fungi, while the enzyme of fungi also can decompose Chinese medicine or
The structural constituent of Chinese medicine slag to generate new ingredient and effect, therefore claims " bi-directional fermentation ", and it is novel that here it is medicinal fungis
(amphicheirality) solid fermentation, abbreviation bi-directional fermentation.The Chinese invention patent of Publication No. CN103908489A discloses a seed ginseng
Chinese medicine ginseng, Radix Astragali are crushed mixed preparing fermentation substrate by the two-way solid fermentation method of stilbene sesame medicinal fungal substance, this method；It will growth
And be stored in the lucidum strain in PDA culture medium and be respectively connected to ferment in culture bottle, obtained ginseng stilbene sesame medicinal fungal substance is anti-oxidant
Activity is higher than former Chinese medicine.The Chinese invention patent of Publication No. CN105176844A discloses a kind of Chinese medicine of Inonotus obliquus
Or Inonotus obliquus is seeded on Chinese medicine or Chinese medicine slag pharmacological property matrix and carries out by the two-way solid fermentation method of Chinese medicine slag, this method
The medicinal fungal substance of two-way solid fermentation, production is non-toxic, and there is good antioxidation, blood sugar reducing function and immunological regulation etc. to make
With.
Monascus can generate a variety of different enzymes in breeding, and certain ingredients of Chinese medicine are under the action of these enzymes
Biochemical reaction occurs, generates new active constituent.There is no literature reported on the two-way solid fermentation process of the Chinese medicine of monascus at present
And its pharmacotoxicological effect of medicinal fungal substance.
Summary of the invention
In view of the deficiencies of the prior art, the purpose of the present invention is to provide a kind of two-way solid fermentations of monascus-Chinese medicine
Technique can improve active ingredients from traditional Chinese medicinal content, conversion generates new active constituent, mitigates toxic side effect.
To achieve the above object, the present invention adopts the following technical scheme:
The present invention provides a kind of two-way solid fermentation process of monascus-Chinese medicine, comprise the steps of:
(1) Chinese medicine of mass ratio 1:(1-3) and rice are crushed by pulverizer, then cross 20 meshes, then plus distilled water it is mixed
Fermentation substrate is obtained after even immersion, fermentation substrate is fitted into the culture bottle of 250mL, is put into high-pressure sterilizing pot in 115-120
It sterilizes at DEG C 2 hours；
(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in and is protected from light ventilated environment
Middle fermentation；
(3) tunning, 60 DEG C of drying are collected after fermentation time.
Radix Salviae Miltiorrhizae is a kind of Chinese medicine that potential applicability in clinical practice is very wide, and step (1) described Chinese medicine is life in 2 years
Red sage root.
Since excessive Chinese medicine can inhibit the growth of monascus, the mass ratio of step (1) Chinese medicine and rice is excellent
It is more appropriate to be selected as 1:1.
When water content is lower, the phase is relatively dry after fermentation for matrix, can inhibit the speed of growth of monascus, therefore step (1)
The water content of the fermentation substrate is preferably within the scope of 110%-120%.
Preferably, the pH value of step (1) described fermentation substrate is 5.
The charge of matrix has larger impact for fermentation results, and confluent cultures bottom of bottle layer is unable to when matrix amount is less,
Monascus slow growth causes active component content lower, when matrix is excessive, although the growth of monascus incipient stage is rapidly,
But since the bed of material is thicker, it is difficult after fermentation the bed of material is long thoroughly, active component content is caused to receive influence, therefore step (1)
The quality of the fermentation substrate is preferably 18-22g.
Preferably, step (2) monascus expands culture processing method particularly includes: 250mL cone is added in 50g rice
In shape bottle, sterilize at 121 DEG C 30min after impregnating 30min with tap water；Test tube slant monascus specie one is taken, use is sterile
Sour water washes down monascus ruber spore, then with aseptic straw inoculating spores suspension 2.5mL, cultivates 6 days in 32 DEG C of incubators, will
Monascus ruber spore on the grain of rice, which is washed down, is made spore suspension.
Mycelium is less when inoculum concentration is very little, is not easy to cover with matrix, and mycelium is excessive, and mycoplasma stickiness increases, and monascus exists
Although early growth is rapid in matrix, there is aging in the later period, slow growth, and since fermentation process can generate heat, when
The very fast heat of rate of rise in temperature is not easy to remove when mycelium is excessive, and the inoculum concentration of step (2) described inoculation is preferably 35%-
42%。
Preferably, step (2) environment temperature for being protected from light ventilated environment controls within the scope of 27-29 DEG C.
Preferably, step (3) fermentation time is 23-25 days.
The invention has the following beneficial effects:
Monascus is seeded in greatly by the two-way solid fermentation process of monascus-Chinese medicine of the invention using two-way solid-fermented technique
On the composite interstitial substance of rice and Chinese medicine composition, while Fermentation Condition of Monascus spp aerial mycelium and metabolite, the enzyme of generation can be urged
The chemical component for changing Chinese medicine converts, and improves active ingredients from traditional Chinese medicinal content, conversion generates new active constituent, mitigates poison
Side effect.The present invention has determined that optimal Chinese medicine and rice quality ratio are 1:1 by a large number of experiments, and best fermentation substrate contains
Water is 110%-120%, and the quality of best fermentation substrate is 18-22g, optimum inoculation amount 35%-42%, optimal yeasting
Temperature is 27-29 DEG C, and optimum fermentation time is 23-25 days, and under the above conditions, the active component content of Chinese medicine can be with
Reach maximum value.
Specific embodiment
Present invention will be further explained below with reference to specific examples.These embodiments are merely to illustrate the present invention and do not have to
In limiting the scope of the invention.
Embodiment 1
A kind of two-way solid fermentation process of monascus-Chinese medicine is present embodiments provided, is comprised the steps of:
(1) 2 years of mass ratio 1:3 raw red sage roots and rice are crushed by pulverizer, then cross 20 meshes, then plus distilled water it is mixed
Fermentation substrate is obtained after even immersion, fermentation substrate is fitted into the culture bottle of 250mL, is put into high-pressure sterilizing pot at 115 DEG C
It sterilizes 2 hours；
(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in and is protected from light ventilated environment
Middle fermentation；
(3) tunning, 60 DEG C of drying are collected after fermenting 23 days.
The water content of step (1) described fermentation substrate is 110% in the present embodiment.
The pH value of step (1) described fermentation substrate is 5 in the present embodiment.
The quality of step (1) described fermentation substrate is 18g in the present embodiment.
Step (2) monascus expands culture processing in the present embodiment method particularly includes: 50g rice is added
In 250mL conical flask, sterilize at 121 DEG C 30min after impregnating 30min with tap water；Test tube slant monascus specie one is taken,
Monascus ruber spore is washed down with sterile sour water, then with aseptic straw inoculating spores suspension 2.5mL, is cultivated in 32 DEG C of incubators
6 days, the monascus ruber spore on the grain of rice is washed down, spore suspension is made.
The inoculum concentration of step (2) described inoculation is 35% in the present embodiment.
Step (2) environment temperature for being protected from light ventilated environment is controlled at 27 DEG C in the present embodiment.
Embodiment 2
A kind of two-way solid fermentation process of monascus-Chinese medicine is present embodiments provided, is comprised the steps of:
(1) 2 years of mass ratio 1:2 raw red sage roots and rice are crushed by pulverizer, then cross 20 meshes, then plus distilled water it is mixed
Fermentation substrate is obtained after even immersion, fermentation substrate is fitted into the culture bottle of 250mL, is put into high-pressure sterilizing pot at 118 DEG C
It sterilizes 2 hours；
(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in and is protected from light ventilated environment
Middle fermentation；
(3) tunning, 60 DEG C of drying are collected after fermenting 25 days.
The water content of step (1) described fermentation substrate is 115% in the present embodiment.
The pH value of step (1) described fermentation substrate is 5 in the present embodiment.
The quality of step (1) described fermentation substrate is 22g in the present embodiment.
Step (2) monascus expands culture processing in the present embodiment method particularly includes: 50g rice is added
In 250mL conical flask, sterilize at 121 DEG C 30min after impregnating 30min with tap water；Test tube slant monascus specie one is taken,
Monascus ruber spore is washed down with sterile sour water, then with aseptic straw inoculating spores suspension 2.5mL, is cultivated in 32 DEG C of incubators
6 days, the monascus ruber spore on the grain of rice is washed down, spore suspension is made.
The inoculum concentration of step (2) described inoculation is 42% in the present embodiment.
Step (2) environment temperature for being protected from light ventilated environment is controlled at 29 DEG C in the present embodiment.
Embodiment 3
A kind of two-way solid fermentation process of monascus-Chinese medicine is present embodiments provided, is comprised the steps of:
(1) 2 years of mass ratio 1:1 raw red sage roots and rice are crushed by pulverizer, then cross 20 meshes, then plus distilled water it is mixed
Fermentation substrate is obtained after even immersion, fermentation substrate is fitted into the culture bottle of 250mL, is put into high-pressure sterilizing pot at 120 DEG C
It sterilizes 2 hours；
(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in and is protected from light ventilated environment
Middle fermentation；
(3) tunning, 60 DEG C of drying are collected after fermenting 24 days.
The water content of step (1) described fermentation substrate is 120% in the present embodiment.
The pH value of step (1) described fermentation substrate is 5 in the present embodiment.
The quality of step (1) described fermentation substrate is 20g in the present embodiment.
Step (2) monascus expands culture processing in the present embodiment method particularly includes: 50g rice is added
In 250mL conical flask, sterilize at 121 DEG C 30min after impregnating 30min with tap water；Test tube slant monascus specie one is taken,
Monascus ruber spore is washed down with sterile sour water, then with aseptic straw inoculating spores suspension 2.5mL, is cultivated in 32 DEG C of incubators
6 days, the monascus ruber spore on the grain of rice is washed down, spore suspension is made.
The inoculum concentration of step (2) described inoculation is 40% in the present embodiment.
Step (2) environment temperature for being protected from light ventilated environment is controlled at 28 DEG C in the present embodiment.
Using Mo Nakelin K and tanshinone IIA content as index, the fermentation of measurement embodiment 1, embodiment 2 and embodiment 3 is produced
The measuring method of the content of active constituent in object, Mo Nakelin K content is as follows: taking 2g monascus-Radix Salviae Miltiorrhizae product by solid-state fermentation molten
In 40mL water, 2h, filtering, filter residue drying are centrifuged in 150rpm；Add 40mL petroleum ether, ultrasonic 1h sloughs pigment, centrifugation, in abandoning
Clearly, precipitating drying；Add 75% ethyl alcohol 30mL, 50 DEG C ultrasound 2 times, each 50min, filtering, merging filtrate, 75% ethyl alcohol is settled to
60mL；300 μ L filtrates are drawn, the dilution of 75% ethyl alcohol is settled to 10mL, obtains sample solution.Using dual-wavelength ultraviolet spectrophotometry
Absorbance (A) of the sample solution at 247nm and 253nm is measured, calculating difference A247-A253 finds out sample according to standard curve
The concentration of Mo Nakelin K in product solution, further calculates out the content of Mo Nakelin K in sample.The measurement of tanshinone IIA content
With the following method: take 2g monascus-Radix Salviae Miltiorrhizae product by solid-state fermentation to be dissolved in 10 times of volumes methanols, 50 DEG C ultrasonic extraction 2 times,
Each 40min, merging filtrate are settled to 20mL.1mL filtrate is taken to cross 0.22 μm of filter membrane, using high performance liquid chromatograph sample introduction point
Analysis, according to the content of calculated by peak area target substance out.Acquired results are as shown in table 1 below:
Table 1
| Test item | Mo Nakelin K content (mg/g) | Tanshinone IIA content (mg/g) |
| Embodiment 1 | 1.31 | 0.98 |
| Embodiment 2 | 1.45 | 1.19 |
| Embodiment 3 | 1.71 | 1.33 |
As can be seen from the above table, the content of Mo Nakelin K and the content of tanshinone IIA in embodiment 1, embodiment 2 and embodiment 3
Higher level is reached, wherein the Mo Nakelin K content of embodiment 3 and tanshinone IIA content are significantly higher than embodiment 1 and reality
Example 2 is applied, illustrates that the technological condition for fermentation of embodiment 3 is the optimal selection of the present invention.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (10)
1. a kind of two-way solid fermentation process of monascus-Chinese medicine, which is characterized in that comprise the steps of:
(1) Chinese medicine of mass ratio 1:(1-3) and rice are crushed by pulverizer, then cross 20 meshes, then plus distilled water it is mixed
Fermentation substrate is obtained after even immersion, fermentation substrate is fitted into the culture bottle of 250mL, is put into high-pressure sterilizing pot in 115-120
It sterilizes at DEG C 2 hours；
(2) it is inoculated into above-mentioned culture bottle after monascus being expanded culture processing, seals, culture bottle is placed in and is protected from light ventilated environment
Middle fermentation；
(3) tunning, 60 DEG C of drying are collected after fermentation time.
2. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (1)
The Chinese medicine is 2 years raw red sage roots.
3. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (1)
The mass ratio of the Chinese medicine and rice is 1:1.
4. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (1)
The water content of the fermentation substrate controls within the scope of 110%-120%.
5. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (1)
The pH value of the fermentation substrate is 5.
6. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (1)
The quality of the fermentation substrate is 18-22g.
7. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, which is characterized in that step (2)
The monascus expands culture processing method particularly includes: 50g rice is added in 250mL conical flask, is impregnated with tap water
Sterilize at 121 DEG C 30min after 30min；Test tube slant monascus specie one is taken, is washed monascus ruber spore with sterile sour water
Under, then with aseptic straw inoculating spores suspension 2.5mL, cultivated 6 days in 32 DEG C of incubators, by the monascus ruber spore on the grain of rice
It washes down and spore suspension is made.
8. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (2)
The inoculum concentration of the inoculation is 35%-42%.
9. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step (2)
The environment temperature for being protected from light ventilated environment controls within the scope of 27-29 DEG C.
10. a kind of two-way solid fermentation process of monascus-Chinese medicine according to claim 1, it is characterised in that: step
(3) fermentation time is 23-25 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111840397A (en) * | 2020-08-24 | 2020-10-30 | 广州三广生物科技有限公司 | Firming and bacteriostatic enzyme liquid and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840397A (en) * | 2020-08-24 | 2020-10-30 | 广州三广生物科技有限公司 | Firming and bacteriostatic enzyme liquid and preparation method thereof |
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Application publication date: 20190308 |