CN109438096A - Selenium-rich richness germanium broccoli sprout soilless culture nutrient fluid and its preparation method - Google Patents
Selenium-rich richness germanium broccoli sprout soilless culture nutrient fluid and its preparation method Download PDFInfo
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- CN109438096A CN109438096A CN201811634392.1A CN201811634392A CN109438096A CN 109438096 A CN109438096 A CN 109438096A CN 201811634392 A CN201811634392 A CN 201811634392A CN 109438096 A CN109438096 A CN 109438096A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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Abstract
The invention belongs to healthy food material production technical fields, and in particular to a kind of preparation method of broccoli sprout soilless culture nutrient fluid.Broccoli sprout rich in sulforaphane is completed by following soilless culture nutrient fluids: potassium dihydrogen phosphate 0-125mg/L, dipotassium hydrogen phosphate 0-150mg/L, zinc sulfate 10-200mg/L, L- selenomethionine 1-50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 1-50mg/L, germanite powder 100-1000mg/L, sodium salicylate 5-100mg/L, calcium propionate 250-2000mg/L, calcium nitrate tetrahydrate 900-1100mg/L, ammonium sulfate 100-250mg/L, potassium sulfate 50-260mg/L, remaining is water.Adjusting pH be 5.5-6.5, with before go supernatant mother liquor sterile water dilute 100 times.
Description
Technical field:
The invention belongs to healthy food material production technical fields, and in particular to a kind of selenium-rich richness germanium broccoli sprout is without soil
Cultivating nutrient solution.
Background technique:
Selenium is a kind of chemical element, and chemical symbol is Se, and period 4 VI A race is located in the periodic table of chemical element, is a kind of
Nonmetalloid.
Selenium is divided into vegetable active selenium and two kinds of inorganic selenium again, and inorganic selenium refers generally to sodium selenite and sodium selenate.Inorganic selenium has
Biggish toxicity, and be not easy to be absorbed, be not suitable for humans and animals and use.
Vegetable active selenium is combined by bioconversion and amino acid, is existed generally in the form of selenomethionine, plant
Active selenium be human and animal allow using selenium source.L- selenium-methyl selenium substituted aminothiopropionic and L- selenomethionine are natures
Only two kinds contain selenoaminoacid, while referred to as third generation selenium-supply kind.
The trophism of selenium
1. selenium has antitumaous effect.Human body selenium deficiency is susceptible to suffer from liver cancer, lung cancer, gastric cancer, the cancer of the esophagus, kidney, prostate cancer, bladder cancer, palace
Neck cancer, leukaemia etc..
2. selenium Wheat Protein.Selenium is best anti-aging substance, if human body lacked selenium will " no longer year
Gently ", it will lead to prematurely senile；
3. selenium can enhance human immunity.Selenium deficiency declines the immunocompetence of human body.
4. selenium plays the role of antagonism harmful heavy metal.Selenium deficiency easily causes the heavy metal poisonings symptom such as lead, arsenic, cadmium.
5. selenium can adjust the absorption and utilization of vitamin A, vitamin C, vitamin E, vitamin K.Selenium deficiency can cause closely
Depending on, cataract, retinopathy, fundus oculi disease, the diseases such as age-related macular degeneration.
6. selenium has the function of the synthesis of regulatory protein matter.Selenium deficiency can cause protein energy shortage property malnutrition, dyeing
Body damage etc..
7. selenium can enhance reproductive function.Selenium deficiency can cause ejaculation and be obstructed, and deformity, conception rate occur for low sperm activity
It reduces, hysteritis disease incidence increases
Etc. symptom.
Selenium element is the required composition of peroxidase in refining, when Se content lowers in sperm, this enzyme
Activity reduces, and spermatoblast membrane lipid peroxidation cannot be inhibited to react, cause Sperm lesion, and dead essence increases, vigor decline.
8. selenium is the important component of the function of muscle, selenium deficiency can make skeletal muscle atrophy and in gray-white stripes, and cardiac muscle occurs
Impaired, cardiac muscle cell's compactness variation, lipid increases, and calcium deposition leads to disease.
So male with greater need for selenium-supply, is largely focused in reproductive organs because supplying intracorporal selenium, and can be with sperm
It excretes together.The resident for especially living in high pollution area lives in the resident in poor selenium area, with greater need for Selenium Supplement.
Since the organ of long-term storage selenium is not present in selenium in human body, selenium needed for body should constantly obtain foot from diet
The balance of the selenium enough measured, selenium concentration has important protective effect and facilitation to the physiological function of many organs, tissue.
When selenium deficiency, human immunological competence's decline is just easily lead to, the 40 of human health and life are threatened
A variety of diseases are all related with human body selenium deficiency, such as cancer, cardiovascular disease, hepatopathy, cataract, pancreatic disease, diabetes, reproductive system
The symptoms such as disease.
For normal, everyone daily total requiremants more than the daily food of Chinese adult 25 microgram of outer selenium-supply has health care
Effect；It is more than outer 50 microgram of selenium-supply of the daily food of selenium deficiency adult or 75 micrograms.Due to selenium deficiency in the soil of China 72%, in day
In right food, the cereal crops Se content such as wheat, rice, corn as staple food crop is below 40 μ g/kg, compatriots
Daily selenium intake lower than world health organisation recommendations 50 μ g minimum intake, day selenium intake there was only 30 ~ 45 μ g,
Lower than countries such as Japan, Canada, the U.S..And selenium-rich rice, 100 micrograms (selenium-rich corn powder), pluck, fish, seafood,
Mushroom, egg, garlic, ginkgo etc. are relatively high containing selenium element, and the crowd of selenium deficiency can suitably increase the food of this respect.
Germanium (old Yi Zuo into Ri) is a kind of chemical element, its chemical symbol is Ge, and atomic number is 32, atomic weight
72.64.It is located at the 4th period, group IVA in the periodic table of chemical element.Natural inorganic germanium is insoluble in water, is not easy to absorb, and has
There is mild toxicity, the micro Germanium that the mankind are absorbed both is from organic germanium.
Organic germanium is divided into synthesis organic germanium, natural organic??, biological organic germanium three classes again.Synthesis organic germanium is ethoxy germanium
The one antivirals such as sesquichloride, i.e. Ge-132, spiral shell germanium, furans germanium derivative, anti-inflammatory, anticancer organic germanium are that have extensively
The compound of pharmacological action, but take and excessively easily cause calcium deficiency；Natural organic?? is edible by extracting in natural plants, or directly,
It is without any side effects to human body；Biological organic germanium be by germanium compound be implanted into organism in, as yeast, bacterium, macro fungi,
Vegetables etc..Organic germanium compounds inhibit the mechanism of tumor promotion to include enhancing immunity of organisms, remove free radical and dash forward with anti-
The many aspects such as change.Be usually oxygen with germanium atom coordination in the organic germanium compounds molecule of many bioactivity, sulphur and nitrogen it
The strong electronegativity atom of class, since they cause the electron cloud around germanium atom to deviate atomic nucleus and shape the absorption of electronics
At a positive center.But when organic germanium compounds encounter tumour cell, normal central can increase the energy of position of tumour cell,
Its mobility is reduced, to play the role of inhibition and kill tumour cell, this is to say organic germanium compounds inhibit tumour
Active biotic potential theory.
Natural organic?? is one of the ingredient of many medicinal plants, and the medicinal plants such as ginseng are rich in organic germanium, has uniqueness
Healthcare function.Since one man of virtue and ability of Japanese scholars shallow well in 1971 has synthesized Ge-132 (GeCH2COOH) 203,
Abbreviation Ge-132, and confirm that it, with anticancer activity, has opened research organic compound from this, it finds efficient, less toxic organic
The prelude of germanium drug.
Organic germanium participates in metabolism in vivo, containing multiple Ge-0 keys, has very strong oxidative dehydrogenation ability.With the blood red egg of human body
White combination guarantees the aerobic metabolism of cell.Since its contained negatively charged oxygen atom can capture the hydrogen in metabolite, part is played
Blood effect is purified, therefore is beneficial to the rehabilitation of the patients such as senile dementia.Organic germanium has green blood stimulation, shows
Increase red blood cell and amount of hemoglobin, stimulating platelet generates, eliminate the lipid attachment on vascular wall, thus to anaemia and
High cholesterol blood lipid and diabetes have certain curative effect.Organic germanium to certain cancerous swellings, immunologic hypofunction, osteoporosis, early ageing,
Vascular hypertension etc. has certain health care effectiveness.
The trophism of germanium
1. activating bioelectricity, promote blood circulation, improves and prevent the sense of discomfort of body
2. protecting red blood cell, the attack of external ray is resisted, is allowed to without damage
3. metabolism, immunity are restored and improve the natural curability of body
4. preventing and treating tumour, anti-inflammatory is antiviral
5. preventing and treating senile osteoporosis
Broccoli, also known as broccoli, general abbreviation broccoli, Xi Lanhua, also known as sprouting broccoli, Brassica oleracea is one
The Western vegetable variety of the newest introduction of kind.Its nutritive value is very high, according to FAO (Food and Agriculture Organization of the United Nation) and Chinese Academy of Medical Sciences's health
Research institute's assay, the every 100 grams of edible parts of broccoli containing vitamin C up to 110 milligrams, it is higher than cabbage and white flower cabbage
One times is tomato more than 4 times；The content of vitamin A is nearly 100 times higher than white flower cabbage up to 3800 international units；Other battalion
It forms point such as a vitamin B, protein and mineral elements content and is all higher than white flower cabbage, wild cabbage and Chinese cabbage.Grinding in recent years
Study carefully discovery, a kind of high-efficiency antioxidant, anti-cancer, anticancer isothiocyanic acid salt compound --- sulforaphane are contained in broccoli, and
And in all presently found brassicaceous vegetables, western indigo plant spends contained sulforaphane highest.The sulforaphane of broccoli seed
Content is 13~14g/kg, is approximately 125 times of sulforaphane highest content in peduncle-growing period for rapeseed and the fresh sample of bud.
Sulforaphane (Sulforaphane, abbreviation SF) is also known as " sulforaphen ", is glucosinolate through myrosin
A kind of isothiocyanate that enzymatic hydrolysis or sour water solution generate, sulforaphane are that the anticancer vigor that finds in vegetables so far is most strong
One of active material, relative molecular mass 177.29, molecular formula C6H11S2NO.It is thin to human breast carcinoma that some researches show that it
Born of the same parents, colon cancer cell, human bladder cancer cell etc. have good inhibiting effect and can induce cancer cell-apoptosis.Glucosinolate
(sulphur glycosides) is widely present in brassicaceous vegetable, especially broccoli sprout, the content of sulphur glycosides be its mature period 40 ~
100 times.
Selenium-rich richness germanium broccoli sprout can be used as bean sprout vegetables, can also dry dozen powder and be added in food, or packaging
At capsule as health food, for improving strengthen immunity, antioxidant and anti-aging, the effect of pre- preventing tumor, while body is supplemented
Body microelement.Main active in view of broccoli sprout is sulforaphane, and the research of the past is although radish sulphur can be improved
Alkane content, but content is atomic in terms of the selenium of sprout and Germanium.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without
It should be considered as recognizing or implying in any form that the information constitutes existing skill already known to those of ordinary skill in the art
Art.
Summary of the invention:
The purpose of the present invention is to provide a kind of selenium-rich richness germanium containing nutrient solution formulas of suitable soilless cultivation broccoli sprout, thus gram
Take above-mentioned defect in the prior art.
To achieve the above object, the present invention provides a kind of broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution,
Include following components in every liter of nutrient solution:
Potassium dihydrogen phosphate 0-125mg/L, dipotassium hydrogen phosphate 0-150mg/L, zinc sulfate 10-200mg/L, L- selenomethionine 1-
50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 1-50mg/L, germanite powder 100-1000mg/L, sodium salicylate 5-100mg/L, third
Sour calcium 250-2000mg/L, calcium nitrate tetrahydrate 500-1100mg/L, ammonium sulfate 100-250mg/L, potassium sulfate 50-260mg/
L, remaining is water.
The technical solution that the present invention further limits are as follows:
Preferably, include following components in every liter of nutrient solution in above-mentioned technical proposal:
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 50-200mg/L, L- selenomethionine
5-50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 5-50mg/L, germanite powder 200-800mg/L, sodium salicylate 10-80mg/L,
Calcium propionate 300-1800mg/L, calcium nitrate tetrahydrate 500-1100mg/L, ammonium sulfate 100-250mg/L, potassium sulfate 50-
260mg/L, remaining is water.
Preferably, include following components in every liter of nutrient solution in above-mentioned technical proposal:
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 100.55mg/L, L- selenomethionine
25mg/L, L- selenium-methyl selenium substituted aminothiopropionic 25mg/L, germanite powder 250mg/L, sodium salicylate 50.00mg/L, calcium propionate
1800.00mg/L, 500.45 mg/L of calcium nitrate tetrahydrate, 120.52 mg/L of ammonium sulfate, 60.44 mg/L of potassium sulfate, remaining is
Water.
Preferably, in above-mentioned technical proposal, the pH value of the nutrient solution is 5.5-6.5.Potassium dihydrogen phosphate in formula and
For dipotassium hydrogen phosphate because being the buffering pair of solution ph, can adjust its dosage according to actual measurement pH numerical value can carry out pH adjusting.
Preferably, in above-mentioned technical proposal, the paving amount of the nutrient solution is 1000mL/ square metres.
A kind of broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution preparation method: it is characterized by: weighing biphosphate
Potassium 0-125mg, dipotassium hydrogen phosphate 0-150mg, zinc sulfate 10-200mg, L- selenomethionine 1-50mg/L, L- selenium-methyl selenium selenium
For cysteine 1-50mg/L, germanite powder 100-1000mg/L, sodium salicylate 5-100mg, calcium propionate 250-2000mg, four hydrations
Calcium nitrate 500-1100mg, ammonium sulfate 100-250mg, potassium sulfate 50-260mg, until adding water to standard in 1L normal capacity device
After the graduation mark of Container mix mother liquor, in use, after taking predetermined volume mother liquor to be diluted with water 100 times mix.
Compared with prior art, the invention has the following beneficial effects:
1, according to plant seedling short cultivation, by measuring the selenium of broccoli sprout and the content of germanium, calculate on this basis and
Each compound amount is adjusted, nutrient solution of the invention is made to obtain effective mass control, ensures the nutrition of broccoli sprouting term growth
Balanced and sprout selenium and Germanium content.
2, a kind of nutrient solution prescription for increasing broccoli sprout selenium and Germanium content has been invented；By going germanium to seek with selenium is removed
The sprout cultivating comparative test of nutrient solution, under the conditions of determining soilless cultivation, broccoli when laying selenium-rich richness germanium containing nutrient solution of the invention
Selenium and Germanium content significantly improve in the broccoli sprout that selenium and Germanium content are cultivated than control in sprout, improve western orchid
The food value of bud seedling.
Specific embodiment:
Specific embodiments of the present invention will be described in detail below, it is to be understood that protection scope of the present invention not by
The limitation of specific embodiment.
Unless otherwise explicitly stated, otherwise in entire disclosure and claims, term " includes " or its change
It changes such as the step of "comprising" or " including " etc. will be understood to comprise statement or component part, and does not exclude other steps
Rapid or other component parts.
Case study on implementation 1,
A kind of broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution formula, in every liter of nutrient solution, component and content are as follows:
Scheme 1: wherein the dosage of potassium dihydrogen phosphate and dipotassium hydrogen phosphate can be adjusted according to actual measurement pH numerical value.
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 100.55mg/L, L- seleno egg
Propylhomoserin 25mg/L, L- selenium-methyl selenium substituted aminothiopropionic 25mg/L, germanite powder 200mg/L, sodium salicylate 50.00mg/L, propionic acid
Calcium 1800.00mg/L, 500.45 mg/L of calcium nitrate tetrahydrate, 120.20 mg/L of ammonium nitrate, 120.52 mg/L of ammonium sulfate, sulphur
Sour 60.44 mg/L of potassium, remaining is water, adjusts pH to 6.0, and as Concentrated stock liquid, after 0.45 μ membrane filtration, 4 DEG C are deposited in short term
It puts 1 week.With preceding take supernatant mother liquor with sterile water dilute 100 times, 1x nutrient solution.
Scheme 2: wherein the dosage of potassium dihydrogen phosphate and dipotassium hydrogen phosphate can be adjusted according to actual measurement pH numerical value.
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 50mg/L, L- selenomethionine
10mg/L, L- selenium-methyl selenium substituted aminothiopropionic 50mg/L, germanite powder 500mg/L sodium salicylate 25.00mg/L, calcium propionate
500.00mg/L, 800.00 mg/L of calcium nitrate tetrahydrate, ammonium nitrate 120.20 mg/L, ammonium sulfate 150mg/L, potassium sulfate
100mg/L, remaining is water, adjusts pH to 6.0, as Concentrated stock liquid, after 0.45 μ membrane filtration, 4 DEG C short-term storage 1 week.With
Before take supernatant mother liquor with sterile water dilute 100 times, 1x nutrient solution.
Scheme 3: wherein the dosage of potassium dihydrogen phosphate and dipotassium hydrogen phosphate can be adjusted according to actual measurement pH numerical value.
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 10-200mg/L, L- seleno egg
Propylhomoserin 50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 10mg/L, germanite powder 900mg/L, sodium salicylate 100mg/L, calcium propionate
1500mg/L, calcium nitrate tetrahydrate 1000mg/L, ammonium sulfate 200mg/L, potassium sulfate 220mg/L, remaining is water, adjusts pH and arrives
6.0, as Concentrated stock liquid, after 0.45 μ membrane filtration, 4 DEG C short-term storage 1 week.With preceding taking supernatant mother liquor to dilute with sterile water
100 times, 1x nutrient solution.
Embodiment 2, broccoli sprout cultivating:
(1) full seed is selected, purity and the higher CDBY-10 broccoli seed not gone mouldy of cleanliness are as bean sprout Miao Pei
The seed educated；
(2) it sterilizes: with the seed 2h of liquor natrii hypochloritis's soaking step (1) of 5g/L concentration, then with aseptic water washing 3-4
It is secondary, obtain disinfection seed；
(3) it takes cleaning non-toxic plastic pallet several, places double gauze or gross porosity paper thereon；
(4) 1 x nutrient solution on the seed yarn releasing cloth disinfected, will be added to soak gauze or paper, control, which adds, to be sought without selenium without germanium
Nutrient solution soaks gauze.No selenium is formulated as follows without germanium containing nutrient solution:
Potassium dihydrogen phosphate 20mg/L, dipotassium hydrogen phosphate 141.61 mg/L, zinc sulfate 100.55mg/L, sodium salicylate 50.00mg/
L, calcium propionate 1800.00mg/L, 500.45 mg/L of calcium nitrate tetrahydrate, 120.20 mg/L of ammonium nitrate, ammonium sulfate 120.52
Mg/L, 60.44 mg/L of potassium sulfate, remaining is water, adjusts pH to 6.0, as Concentrated stock liquid, after 0.45 μ membrane filtration, and 4 DEG C
Short-term storage 1 week.With preceding take mother liquor sterile water dilute 100 times, 1x nutrient solution.
(5) 16 hours/dark of illumination 8 hours, cultivation temperature is 25 DEG C, relative humidity 60% is kept, after continuous cultivation 7 days
Harvest.
Case study on implementation 3, the measurement of selenium element in broccoli sprout
According to the People's Republic of China (PRC) " measurement of national food safety standard selenium in food " (GB5009.93-2017) method
Measurement.Determining instrument used is Atomic Fluorescence Spectrometer (matching selenium hollow cathode lamp).
1) prepared by sample: broccoli bean sprout fresh sample is eluted with water, dries, taking edible part that homogenate is made.
2) sample digestion: wet digestion.
It weighs solid sample 0.5g ~ 3g (being accurate to 0.001g) or accurately pipettes liquor sample 1.00mL ~ 5.00mL, be placed in
In conical flask, add 10mL nitric acid-perchloric acid mixed acid (9+1) and several beades, the cold digestion of cap upper surface ware is overnight.Next day in
It is heated on electric hot plate, and adds nitric acid in time.When solution becomes Clear colourless and generates with white cigarette, it is further continued for being heated to remaining
Remaining volume is 2mL or so, can't be evaporated.It is cooling, then plus 5mL hydrochloric acid solution (6mol/L), continue to be heated to solution to become clear
It is bright colourless and occur with white cigarette.
It is transferred in 10mL volumetric flask, is added 2.5mL potassium ferricyanide solution (100g/L) after cooling, with water constant volume, mix
It is to be measured.Reagent blank test is done simultaneously.
3) it measures
Instrument performance is adjusted to optimum state.Reference conditions are as follows: 340 V of negative high voltage；100 mA of lamp current；Atomization temperature 800
℃；The high 8mm of furnace；Flow rate of carrier gas 500mL/min；Shield gas flow speed 1000mL/min；Measurement method calibration curve method；Reading mode
Peak area；Delay time 1s；Reading duration 15s；Liquid feeding time 8s；Sampling volume 2mL.
The production of standard curve: with hydrochloric acid solution (5+95) for current-carrying, sodium borohydride aqueous slkali (8g/L) is reducing agent, even
Zero pipe sample introduction of continuous standard series will mark selenium standard serial solution by mass concentration from low to high after stable reading
Sequence is directed respectively into instrument, measures its fluorescence intensity, and using mass concentration as abscissa, fluorescence intensity is ordinate, makes standard
Curve.
Specimen Determination: under experiment condition identical with measurement standard serial solution, by blank solution and sample solution point
Not Dao Ru instrument, survey its fluorescent value intensity, it is quantitative compared with standard series.
4) statement of the measurement analysis result of selenium in food
The content of selenium calculates as follows in sample:
,
In formula:
X --- the content of selenium in sample, unit be milligrams per kilogram or milligrams per liter (mg/kg or mg/L);
ρ --- the mass concentration of selenium in sample solution, unit are micro- gram per liter (μ g/L);
ρ 0 --- the mass concentration of selenium in blank solution, unit are micro- gram per liter (μ g/L)；
V --- sample digestive juice total volume, unit are milliliter (mL)；
M --- sample sample weighting amount pipettes volume, unit be gram or milliliter (g or mL)；
1000 --- conversion coefficient.
As Se content>=1.00 mg/kg (or mg/L), calculated result retains three effective digitals, when Se content<1.00
When mg/kg (or mg/L), calculated result retains two effective digitals.
5) absolute difference of measurement precision and other measurement results independent twice obtained under the conditions of repeatability must not
More than the 20% of arithmetic mean of instantaneous value.When sample weighting amount is 1g (or 1mL), and constant volume is 10mL, the detection of method is limited to
0.002mg/kg (or 0.002mg/L) is quantitatively limited to 0.006mg/kg (or 0.006mg/L).
6) test result: the broccoli sprout selenium-rich richness germanium soilless culture nutrient fluid, which has, significantly improves broccoli sprout
The organic selenium content of dry product, the results are shown in Table 1.
Case study on implementation 4, the measurement of Germanium content in broccoli sprout
The measurement of Ge content realizes (the first method atomic fluorescence according to standard method as defined in national standard GB/T 5009.151-2003
Spectroscopic methodology), concrete operations are as follows:
1) instrument
Dual channel atomic fluorescence photometers, electric hot plate.
2) analytical procedure
(1) prepared by sample
Fresh broccoli bean sprout takes edible part that homogenate is made.
(2) total germanium determination in sample
1. sample digests
Fresh sample 5.00g is weighed in 150m L conical flask, adds 3 beades, adds 10mL-15mL nitric acid, 2.5 mL sulfuric acid, lid table
Face ware is stood overnight.Next day is placed on electric hot plate and heats.During heating, as found, solution becomes brown, then needs taper
Bottle is removed, and a small amount of nitric acid is added.When solution starts to emit white cigarette, conical flask is removed, after slightly cold, slowly plus 1 mL peroxidating of people
Hydrogen, heating, is repeated twice, and to remove remaining nitric acid, and is heated to white cigarette appearance.Conical flask is removed, it is cooling.Solution is moved
In 25 mL volumetric flask of people, adds 5 mL phosphoric acid of people, be diluted with water to scale, shake up.Reagent blank test is done simultaneously.It is to be measured.
2. standard series is prepared: 500ng/mL germanium standard solution 1.00,2.00,4.00,6.00 is drawn respectively,
8.00 mL add 10 mL phosphoric acid of people, are diluted with water to scale in 50 mL volumetric flasks, mix.Respectively it is equivalent to germanium concentration
10.00, 20.00, 40.00, 60.00, 80.00 ng/mL。
3. instrument is referring to condition: negative high voltage: 410 V；Lamp current: 80 mA；Atomizer: temperature 8750C, height 8.5
mm；Hydrogen flow rate: 450 mL/min of carrier gas shields 1000 mL/min of gas；Measurement method: calibration curve method；Reading mode: peak
Area；Delay time: 1.0；Reading duration: 10.0 S；The solution of potassium borohydride liquid feeding time: 8.0 s；Standard liquid or sample liquid liquid feeding
Volume: 2 mL.
3) measuring method
Concentration mensuration mode measures: setting instrument optimum condition, after furnace temperature is gradually risen to required temperature, stablizes 10m in-
Start to measure after 20m in.The continuous zero pipe sample introduction for using standard series turns the measurement of people's standard series, draws after stable reading
Standard curve processed.Turn people's sample measurement, measures sample blank and sample digestive juice, every all Ying Qingxi before surveying different samples respectively
Sample injector.Specimen Determination result is calculated by formula (1).
4) result calculates
It is calculated by formula (1):
X=1000 x 1000 --- --- ----(1) of [(mono- A2 of A1) x V x 1000]/m x
In formula:
The content of germanium in X-sample, unit are milligrams per kilogram (milligrams per liter [mg/kg (mg/L)]；
A1-sample digestive juice measures concentration, and unit is nanograms per milliliter (ng/mL)；
A2-reagent blank liquid measures concentration, and unit is nanograms per milliliter (ng/mL)；
V-sample digestive juice total volume, unit are milliliter (mL)；
M-sample mass (volume), unit are gram (milliliter) [g/ (mL)].
Note 1: the content of total germanium and the content of inorganic Ge are calculated by formula (1), and the difference of the two is as organic multiplied by 2.338
The content of germanium (GE-132).
Note 2: dry weight is 75% calculating with weight in wet base water content.
5) precision
The absolute difference of the measurement result independent twice obtained under the conditions of repeatability must not exceed the 5% of arithmetic mean of instantaneous value.
6) test result: the broccoli sprout selenium-rich richness germanium soilless culture nutrient fluid, which has, significantly improves broccoli sprout
The organic germanium content of dry product, the results are shown in Table 1.
The measurement analysis result of table 1
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These are retouched
It states and is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can carry out very much
Change and changes.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and in fact
Border application, so that those skilled in the art can be realized and utilize a variety of different exemplary embodiment party of the invention
Case and various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (6)
1. a kind of broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution includes following components in every liter of nutrient solution:
Potassium dihydrogen phosphate 0-125mg/L, dipotassium hydrogen phosphate 0-150mg/L, zinc sulfate 10-200mg/L, L- selenomethionine 1-
50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 1-50mg/L, germanite powder 100-1000mg/L, sodium salicylate 5-100mg/L, third
Sour calcium 250-2000mg/L, calcium nitrate tetrahydrate 900-1100mg/L, ammonium sulfate 100-250mg/L, potassium sulfate 50-260mg/
L, remaining is water.
2. broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution according to claim 1, it is characterised in that: sought at every liter
Include following components in nutrient solution:
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 50-200mg/L, L- selenomethionine
5-50mg/L, L- selenium-methyl selenium substituted aminothiopropionic 5-50mg/L, germanite powder 200-800mg/L, sodium salicylate 10-80mg/L,
Calcium propionate 300-1800mg/L, calcium nitrate tetrahydrate 900-1100mg/L, ammonium sulfate 100-250mg/L, potassium sulfate 50-
260mg/L, remaining is water.
3. broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution according to claim 1, it is characterised in that: sought at every liter
Include following components in nutrient solution:
Potassium dihydrogen phosphate 10-125mg/L, dipotassium hydrogen phosphate 10-150mg/L, zinc sulfate 100.55mg/L, L- selenomethionine
25mg/L, L- selenium-methyl selenium substituted aminothiopropionic 25mg/L, germanite powder 250mg/L, sodium salicylate 50.00mg/L, calcium propionate
1800.00mg/L, 500.45 mg/L of calcium nitrate tetrahydrate, 120.52 mg/L of ammonium sulfate, 60.44 mg/L of potassium sulfate, remaining is
Water.
4. broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution described in -3 any claims according to claim 1, special
Sign is: the pH value of the nutrient solution is 5.5-6.5.
5. broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution described in -3 any claims according to claim 1, special
Sign is: the paving amount of the nutrient solution is 1000mL/ square metres.
6. a kind of broccoli sprout soilless cultivation selenium-rich richness germanium containing nutrient solution preparation method: it is characterized by: weighing potassium dihydrogen phosphate
0-125mg, dipotassium hydrogen phosphate 0-150mg, zinc sulfate 10-200mg, L- selenomethionine 1-50mg/L, L- selenium-methyl selenium seleno
Cysteine 1-50mg/L, germanite powder 100-1000mg/L, sodium salicylate 5-100mg, calcium propionate 250-2000mg, four hydration nitre
Sour calcium 900-1100mg, ammonium sulfate 100-250mg, potassium sulfate 50-260mg, until adding water to standard appearance in 1L normal capacity device
After the graduation mark of measuring device mix mother liquor, in use, after taking predetermined volume mother liquor to be diluted with water 100 times mix.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113812311A (en) * | 2021-08-25 | 2021-12-21 | 苏州虞景智田农业科技有限公司 | Preparation method of selenium-rich broccoli sprouting vegetable |
Citations (5)
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|---|---|---|---|---|
| CN102701844A (en) * | 2012-05-21 | 2012-10-03 | 成钢 | Rich-selenium-germanium trace element nanometer nutrition fertilizer for vegetable and fruit soilless culture |
| CN104496632A (en) * | 2014-11-17 | 2015-04-08 | 浙江大学 | Method for ensuring safe output of paddy planted in lead mild-moderate polluted soil and used preparation |
| CN105000939A (en) * | 2015-08-05 | 2015-10-28 | 天津农学院 | Soilless culture nutrient solution formula for blueberries |
| CN105103876A (en) * | 2015-08-20 | 2015-12-02 | 孙群 | Selenium-rich broccoli cultivating method |
| CN108530197A (en) * | 2018-06-25 | 2018-09-14 | 合肥雪立歆农业科技有限公司 | One kind is rich in microelement foliage fertilizer and preparation method thereof |
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2018
- 2018-12-29 CN CN201811634392.1A patent/CN109438096A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102701844A (en) * | 2012-05-21 | 2012-10-03 | 成钢 | Rich-selenium-germanium trace element nanometer nutrition fertilizer for vegetable and fruit soilless culture |
| CN104496632A (en) * | 2014-11-17 | 2015-04-08 | 浙江大学 | Method for ensuring safe output of paddy planted in lead mild-moderate polluted soil and used preparation |
| CN105000939A (en) * | 2015-08-05 | 2015-10-28 | 天津农学院 | Soilless culture nutrient solution formula for blueberries |
| CN105103876A (en) * | 2015-08-20 | 2015-12-02 | 孙群 | Selenium-rich broccoli cultivating method |
| CN108530197A (en) * | 2018-06-25 | 2018-09-14 | 合肥雪立歆农业科技有限公司 | One kind is rich in microelement foliage fertilizer and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812311A (en) * | 2021-08-25 | 2021-12-21 | 苏州虞景智田农业科技有限公司 | Preparation method of selenium-rich broccoli sprouting vegetable |
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