CN109432501A - Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic - Google Patents

Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic Download PDF

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Publication number
CN109432501A
CN109432501A CN201811276705.0A CN201811276705A CN109432501A CN 109432501 A CN109432501 A CN 109432501A CN 201811276705 A CN201811276705 A CN 201811276705A CN 109432501 A CN109432501 A CN 109432501A
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CN
China
Prior art keywords
composite
fibrous scaffold
chitosan
purposes
composite material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811276705.0A
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Chinese (zh)
Inventor
李青峰
高博闻
高鸣
高一鸣
谢峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
Original Assignee
Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine filed Critical Ninth Peoples Hospital Shanghai Jiaotong University School of Medicine
Priority to CN201811276705.0A priority Critical patent/CN109432501A/en
Publication of CN109432501A publication Critical patent/CN109432501A/en
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3612—Cartilage, synovial fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/20—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3616—Blood, e.g. platelet-rich plasma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3645—Connective tissue
    • A61L27/3654—Cartilage, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/52—Hydrogels or hydrocolloids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus

Abstract

It include cartilage particles in the composite material the invention proposes a kind of purposes of composite fibrous scaffold in the composite material for preparing nasal plastic.The composite fibrous scaffold is specifically chosen chitosan composite fiber bracket.It is compared compared to using self fascia package cartilage particles, the composite fibrous scaffold in the present invention provides suitable extracellular matrix to cartilage particles, therefore cartilage particles survival rate is higher；And composite fibrous scaffold is simple for production, the operating time is short, will not form additional donor site damage and scar because cutting human body fascia.The mixture of cartilage particles and chitosan composite fiber bracket composition has certain mobility, is injected to bridge of the nose from closure approach by syringe, easy to operate.

Description

Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic
Technical field
The present invention relates to shaping material field, a kind of composite fibrous scaffold is particularly related in the composite material for preparing nasal plastic In purposes.
Background technique
Nose occupies facial critical positions, is the important symbol that facial stereo coordinates beauty.Asians is short and low flat because of its It is nose-shaped, it usually needs to increase the height and length of bridge of the nose.Therefore augmentation rhinoplasty is art formula most commonly seen in nasal plastic.Plastic surgery Doctor increases bridge of the nose height, such as silica gel, varicosity material using non-autologous implantation material earliest.Since these implantation materials are raw Object compatibility is poor, therefore can bring many complication when in use, be even pierced by such as autoimmune response, implantation material shift, Infection etc..In recent years, self cartilage is because its histocompatbility is good, do not generate rejection, infection and exposing ratio are low, source is rich Richness, thus become augmentation rhinoplasty conventional material.Currently used cartilage augmentation rhinoplasty is after carving monolith costal cartilage, in open approach Under conditions of be placed on bridge of the nose, to increase bridge of the nose height.However the monolith costal cartilage item after carving is easy to occur bending and deformation, Cause bridge of the nose crooked, shape is bad, and absorptivity is higher；It is concurrent to will lead to nose pillar notch with open approach progress nasal plastic Disease, such as scar is broadening, recess, forms incisura, pigmentation.
The segmental defect using monolith costal cartilage can be improved using self cartilage particles augmentation rhinoplasty, be such as unlikely to deform, plasticity OK etc., but need for cartilage to be cut to tiny fragment, how to concentrate fixed and shape, then it is very difficult.Scholar attempts using self Fascia wraps up the self cartilage particles of diameter 0.5-1mm, the padded bridge of the nose under open approach.Since cartilage particles lack extracellularly Matrix provides growing environment, and new vessels can not all cartilage particles of nutrition, therefore the absorptivity of cartilage particles is still higher, Some patientss at a specified future date need to go operation to adjust again.
To sum up, it needs to find a kind of new material, as carrier, cartilage particles can be concentrated and be formed, while can mentioned again Cartilage cell epimatrix is supplied, vascularization quickening, survival rate raising are allowed to, to reduce the deformation of bridge of the nose graft.
Summary of the invention
The present invention proposes a kind of purposes of composite fibrous scaffold in the composite material for preparing nasal plastic, solves existing skill The new vessels occurred after nasal plastic in art are unable to satisfy cartilage particles nutrition, so that cartilage particles absorptivity is higher, Jin Erxu Want the problem of performing the operation again at a specified future date.
The technical scheme of the present invention is realized as follows:
A kind of purposes of composite fibrous scaffold in the composite material for preparing nasal plastic includes cartilage in the composite material Particle.
Wherein composite fibrous scaffold is to refer to composite fibrous scaffold in ZL2017106649584.
The composite fibrous scaffold is chitosan composite fiber bracket as a preferred technical solution,.
The mass ratio of the cartilage particles and chitosan composite fiber bracket is 1:1-2 as a preferred technical solution,.
The preparation method of chitosan composite fiber bracket as a preferred technical solution, comprising:
The water soluble chitosan of deacetylation is dissolved in physiological saline, chitosan gel rubber is obtained；
The chitosan gel rubber and venous blood are mixed, mixed liquor is obtained；
It is centrifuged the mixed liquor before the mixed liquor blood coagulation, obtains chitosan composite fiber bracket.
The chitosan is the water soluble chitosan that deacetylation is more than or equal to 90% as a preferred technical solution,.More The preferably water soluble chitosan of deacetylation 90%.
The mass fraction of the chitosan gel rubber is 7%-15% as a preferred technical solution,.More preferably quality The chitosan gel rubber that score is 10%.
The volume ratio of the chitosan gel rubber and venous blood is 1:1-2 as a preferred technical solution,.
The diameter of the cartilage particles is 0.5-1.2mm as a preferred technical solution,.
Beneficial effect
(1) it is compared compared to using self fascia package cartilage particles, the composite fibrous scaffold in the present invention is to cartilage particles Suitable extracellular matrix is provided, therefore cartilage particles survival rate is higher；And composite fibrous scaffold is simple for production, the operating time It is short, additional donor site damage and scar will not be formed because cutting human body fascia.
(2) mixture of cartilage particles and chitosan composite fiber bracket composition has certain mobility, passes through injection Device is injected to bridge of the nose from closure approach, easy to operate.
(3) composite fibrous scaffold that the present invention uses has reticular structure, pore diameter 20-30um, and new vessels hold It is easily grown into from hole, so that nutrition cartilage particles, increase the survival rate of cartilage, reduces operability at a specified future date.
(4) composite fibrous scaffold of the invention is rich in a variety of growth factors, gives the cartilage cell in cartilage particles tissue Proliferation, the good environment of differentiation.
(5) high polymer adjuvant of the chitosan as this composite fibrous scaffold has good biocompatibility, can be in human body Interior safety injection.
Detailed description of the invention
It, below will be to embodiment in order to illustrate more clearly of embodiment of the present invention or technical solution in the prior art Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is some embodiments of the invention, for those of ordinary skill in the art, in the premise of not making the creative labor property Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1: for the compound bio bracket electromicroscopic photograph (precision 100um) in the present invention；
Fig. 2: for the compound bio bracket electromicroscopic photograph (precision 50um) in the present invention；
Fig. 3: for the structural schematic diagram of the particle cartilage in the present invention；
Fig. 4: for the mixing schematic diagram of particle cartilage and chitosan compound biological bracket in the present invention；
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects It encloses.
Reagent used in following embodiments is commercially available.
Embodiment 1
A kind of purposes of composite fibrous scaffold in the composite material for preparing nasal plastic includes cartilage in the composite material Grain.
The composite fibrous scaffold selected in the present embodiment is chitosan composite fiber bracket.
The preparation method of the chitosan composite fiber bracket, specifically includes:
(1) the water soluble chitosan pulvis of deacetylation 90% is configured to mass fraction using physiological saline as solvent For 10% chitosan gel rubber；
(2) venous blood is taken, is uniformly mixed chitosan gel rubber by volume 1:1 with blood before blood coagulation, the time controls 2 In minute, mixed liquor is obtained；
(3) it is layered after being placed in centrifuge 400g centrifugation 10min.Lower layer's red blood cell is removed, stirs evenly, chitosan can be obtained Composite fibrous scaffold, such as Fig. 1-2.
(4) self cartilage is chopped into diameter is about 1mm cartilage particles, such as Fig. 3.
(5) chitosan composite fiber bracket is mixed with cartilage particles according to volume ratio 1:1 to get such as Fig. 4.
The water soluble chitosan that deacetylation is greater than 90% can be selected in chitosan in the present embodiment.Shell in the present embodiment The mass fraction of polysaccharide gel can select in 7%-15%.The volume ratio of chitosan gel rubber and venous blood can be adjusted in 1:1-2 It is whole.
Chitosan composite fiber bracket obtained above and cartilage particles can be used to bridge of the nose transplanting.Intranasal approach is selected, It moves under water separation along bridge of the nose cartilage surface, when separation to nasal bone separates nasal bone periosteum, and range is wide down toward nose up to nasion portion Degree is slightly larger than bridge of the nose width.Guide is inserted into from notch up to the nasion, level is nasal bone subperiosteum.By syringe along guide It is injected to bridge of the nose.Filled chitosan composite fibrous scaffold and cartilage particles in the syringe.
The present invention applies composite fibrous scaffold in the composite material of nasal plastic, is because the material has following spy Property: the natural extracellular matrix of 1 simulation；2 apertures are about 20-30um, are grown into convenient for new vessels；3 itself can secretory cells because Son promotes angiogenesis；4 good biological degradation rates；5 stickiness and mobility appropriate, can not only bond broken cartilage particles, but also Cartilage particles/composite fibrous scaffold composite graft can be enable by syringe, bridge of the nose is injected into and completes transfer operation.
Since composite fibrous scaffold provides suitable extracellular matrix to cartilage particles, cartilage particles survival rate compared with It is high；And composite fibrous scaffold is simple for production, the operating time is short, will not form additional donor site damage and scar because cutting human body fascia Trace.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.

Claims (8)

  1. It include cartilage in the composite material 1. a kind of purposes of composite fibrous scaffold in the composite material for preparing nasal plastic Grain.
  2. 2. a kind of purposes of the composite fibrous scaffold according to claim 1 in the composite material for preparing nasal plastic, special Sign is that the composite fibrous scaffold is chitosan composite fiber bracket.
  3. 3. a kind of purposes of the composite fibrous scaffold according to claim 2 in the composite material for preparing nasal plastic, special Sign is that the mass ratio of the cartilage particles and chitosan composite fiber bracket is 1:1-2.
  4. 4. a kind of purposes of the composite fibrous scaffold according to claim 2 in the composite material for preparing nasal plastic, special Sign is, the preparation method of chitosan composite fiber bracket, comprising:
    The water soluble chitosan of deacetylation is dissolved in physiological saline, chitosan gel rubber is obtained；
    The chitosan gel rubber and venous blood are mixed, mixed liquor is obtained；
    It is centrifuged the mixed liquor before the mixed liquor blood coagulation, obtains chitosan composite fiber bracket.
  5. 5. a kind of purposes of the composite fibrous scaffold according to claim 4 in the composite material for preparing nasal plastic, special Sign is that the chitosan is the water soluble chitosan that deacetylation is more than or equal to 90%.
  6. 6. a kind of purposes of the composite fibrous scaffold according to claim 4 in the composite material for preparing nasal plastic, special Sign is that the mass fraction of the chitosan gel rubber is 7%-15%.
  7. 7. a kind of purposes of the composite fibrous scaffold according to claim 4 in the composite material for preparing nasal plastic, special Sign is that the volume ratio of the chitosan gel rubber and venous blood is 1:1-2.
  8. 8. a kind of purposes of the composite fibrous scaffold according to claim 4 in the composite material for preparing nasal plastic, special Sign is that the diameter of the cartilage particles is 0.5-1.2mm.
CN201811276705.0A 2018-10-30 2018-10-30 Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic Pending CN109432501A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811276705.0A CN109432501A (en) 2018-10-30 2018-10-30 Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811276705.0A CN109432501A (en) 2018-10-30 2018-10-30 Purposes of the composite fibrous scaffold in the composite material for preparing nasal plastic

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CN109432501A true CN109432501A (en) 2019-03-08

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103877615A (en) * 2014-03-18 2014-06-25 北京大学第三医院 Cartilage tissue engineering bracket and preparation method thereof
CN105079879A (en) * 2015-01-22 2015-11-25 中国人民解放军第二军医大学 Method for constructing chondroid transplant by adopting broken cartilages
CN106492285A (en) * 2016-10-31 2017-03-15 广州昕生医学材料有限公司 Injectable Acellular cartilaginous matrix particulate and its application in implant
CN106999635A (en) * 2014-12-11 2017-08-01 苏黎世联邦理工学院 Repair of cartilage graft support and its manufacture method
CN107412878A (en) * 2017-08-07 2017-12-01 上海交通大学医学院附属第九人民医院 Composite fibrous scaffold and preparation method thereof
CN108024825A (en) * 2015-09-25 2018-05-11 斯贝洛克斯公司 Nasal implants and system and application method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103877615A (en) * 2014-03-18 2014-06-25 北京大学第三医院 Cartilage tissue engineering bracket and preparation method thereof
CN106999635A (en) * 2014-12-11 2017-08-01 苏黎世联邦理工学院 Repair of cartilage graft support and its manufacture method
CN105079879A (en) * 2015-01-22 2015-11-25 中国人民解放军第二军医大学 Method for constructing chondroid transplant by adopting broken cartilages
CN108024825A (en) * 2015-09-25 2018-05-11 斯贝洛克斯公司 Nasal implants and system and application method
CN106492285A (en) * 2016-10-31 2017-03-15 广州昕生医学材料有限公司 Injectable Acellular cartilaginous matrix particulate and its application in implant
CN107412878A (en) * 2017-08-07 2017-12-01 上海交通大学医学院附属第九人民医院 Composite fibrous scaffold and preparation method thereof

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Application publication date: 20190308

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