CN109406782A - A kind of immunochromatography detector bar and preparation method thereof of quick diagnosis and monitoring lung cancer - Google Patents
A kind of immunochromatography detector bar and preparation method thereof of quick diagnosis and monitoring lung cancer Download PDFInfo
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- CN109406782A CN109406782A CN201811225079.2A CN201811225079A CN109406782A CN 109406782 A CN109406782 A CN 109406782A CN 201811225079 A CN201811225079 A CN 201811225079A CN 109406782 A CN109406782 A CN 109406782A
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- lung cancer
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- detector bar
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57423—Specifically defined cancers of lung
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/70—Mechanisms involved in disease identification
- G01N2800/7023—(Hyper)proliferation
- G01N2800/7028—Cancer
Abstract
The immunochromatography detector bar and preparation method thereof of a kind of quick diagnosis provided by the invention and monitoring lung cancer, using the tumor markers of double antibody sandwich method principle detection Small Cell Lung Cancer and/or the tumor markers (antigen) of non-small cell tumour, to judge whether lung cancer occurs.Immunochromatography detector bar provided by the invention is easy to carry and saves;Easy to operate, method is stablized, and tests in completion in 10 minutes.Have the characteristics that can to quantify, sxemiquantitative and qualitative detection, can reflect the concentration range and variation tendency of the tumor markers of lung cancer, hence it is evident that facilitate the use of clinical diagnosis and treatment detection.Test equipment also can be used in the present invention, the tumor markers (antigen) in test sample that can be simple and quick.
Description
It is on May 11st, 2016 that the application, which is the applying date, entitled " a kind of application No. is 201610307049.0
Quick diagnosis and monitoring lung cancer immunochromatography detector bar and preparation method thereof " divisional application, apply for artificial Lushi laboratory
Company;Nanjing Road eozoon Science and Technology Ltd..
Technical field
The invention belongs to biological medicine and technical field of medical examination more particularly to a kind of quick diagnosis and monitor lung cancer
Immunochromatography detector bar and preparation method thereof.
Background technique
Lung cancer is the first place of the current whole world cancer cause of the death, and China is also the first in the world lung cancer big country now.Lung cancer is
Cause the highest cancer of the death rate, in past 30 years, Chinese Lungs mortality of carcinoma increases 464.15%, non-small cell
The 85% of lung cancer (NSCLC) Zhan Suoyou lung cancer ratio.Squamous cell carcinoma, gland cancer and large cell carcinoma belong to non-small cell lung cancer
Subtype.Small Cell Lung Cancer proportion is not high, but pernicious intensity is very high.Chinese's lung cancer incidence average annual growth 20%.In mistake
The survival rate for the 20 years lung cancer sufferers gone is very low.
Early detection lung cancer is the best way of fight lung cancer.Have iconography currently on the market to detect, such as CT scan,
Nuclear-magnetism (MRI) scanning it can be seen that lung cancer happening part, size, and whether the information such as transferred, metastasis site, but this
Kind method cannot be used frequently, and expense larger to the personal radiation of patient is costly.In addition, lung tissue Puncture cytology
Inspection also has important clinical value to the early diagnosis of lung cancer and diagnosis rate, takes In vivo detection sample to pass through using operation lung puncture
Piece diagnosis is tested by veteran pathology doctor after fixed, embedding, slice, dyeing, cytology detects lung cancer largely
Diagnostic result could accurately be provided by relying on professional experiences, and it is cumbersome, obtain that the diagnostic result period is longer, while patient
Body & mind injury is larger, is not able to satisfy the needs that people quickly detect.
The detection of neoplastic hematologic disorder marker in the early diagnosis of lung cancer and early treatment there are important references to be worth, but by
In the lung carcinoma cell polymorphism the characteristics of, existing single lung cancer neoplastic hematologic disorder marker sensitivity is lower, for example, in recent years, inspection
The immunologic detection method for surveying marker for lung cancer has chemoluminescence method and enzyme linked immunosorbent assay, although these methods have quickly, accurately, specifically
Property high feature, but operation needs detection device and professional to operate, and sensitivity is also to be improved.
Summary of the invention
In view of this, the purpose of the present invention is to provide the immunochromatography detector bar of a kind of quick diagnosis and monitoring lung cancer,
Make detector bar sensitivity with higher and accurate testing result, while qualitative and quantitative detection can also be completed.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides the immunochromatography detector bar of a kind of quick diagnosis and monitoring lung cancer, including bottom plate, the bottom plates
On from bottom to top it is secondary be sample uptake zone, solid phase labelling antibody district, reaction zone and suction zones, the reaction zone be equipped with detection line T
Nature controlling line C, the detection line T are provided with one or two, the nature controlling line C setting one or more;The detection line packet
The corresponding antibody of tumor markers for being had detection Small Cell Lung Cancer and/or non-small cell lung cancer;The solid phase labelling antibody district
There is the corresponding labelled antibody of tumor markers of detection Small Cell Lung Cancer and/or non-small cell lung cancer.
Preferably, before the tumor markers SCLC neuralization enolase NEC, gastrin releasing peptide of the Small Cell Lung Cancer
Body pro-GRP.
Preferably, the tumor markers NSCLC antigen of the non-small cell lung cancer be cyokeratin antigens c YFRA 21-1,
Phosphorus shape cellular antigens SCC or value of tissue polypeptide specific antigen TPS.
Preferably, the marker of the labelled antibody is gold particle, enzyme, light or carbon granules.
Preferably, the coated concentration that coated tumor markers correspond to antibody in the detection line is 1~3mg/ml.
Preferably, the immunoassay strip further includes the plastic housing for containing detector bar.
Preferably, the sample that the sample uptake zone absorbs is blood, serum or blood plasma.
Preferably, the colour developing situation of the detection line T detects by an unaided eye or is quantified according to the data that reader measures
Testing result, or do not compareed with the intensity of check plot nature controlling line C, obtain half-quantitative detection result.
The present invention also provides the immunochromatography detector bars of quick diagnosis and monitoring lung cancer described in above-mentioned technical proposal
Preparation method, characterized in that it comprises the following steps:
1) labelled antibody is dissolved in buffer, obtains diluted labelled antibody, by the diluted labelled antibody
It is sprayed on immobilon-p, e.g., all-glass paper.Then it is dried in vacuum desiccator;
2) coated antibody solution is lined into the detection zone on reaction zone and forms T line, the reaction zone of scribing line is placed on 25~
It is dried 10~20 minutes under 37 DEG C of environment;
3) according to from top to bottom after after sample uptake zone, solid phase labelling antibody district, reaction zone and suction zones being cut
Sequence be sequentially fixed on bottom plate, suction zones are sticked in upper end again, are cut into item, obtain immunoassay strip;
The immunochromatography detector bar of a kind of quick diagnosis provided by the invention and monitoring lung cancer, including bottom plate, the bottom plate
On it is secondary from bottom to top be once provided with sample uptake zone, solid phase labelling antibody district, reaction zone and suction zones, the reaction zone is equipped with
Detection line T and nature controlling line C, the detection line T are provided with one or two, the nature controlling line C setting one or more;The inspection
Survey line is coated with the corresponding antibody of tumor markers of detection Small Cell Lung Cancer and/or non-small cell lung cancer;The solid phase labelling
Antibody district has the corresponding labelled antibody of tumor markers of detection Small Cell Lung Cancer and/or non-small cell lung cancer.The present invention uses
Double antibody sandwich method principle detects the tumor markers of lung cancer, and the present invention selects suitable lung cancer tumor marker, to adopt simultaneously
With U.S. JAJ international, the production of Inc.San Diego company has and each tumor markers antigen high special
Property antibody obtain with highly sensitive immunoassay strip.The immunoassay strip is easy to carry and saves;It is easy to operate, side
Method is stable, can individually test, and tests in completion in 10 minutes.Have the characteristics that can quantitative and semi-quantitative measurement, can reflect lung cancer
Tumor markers concentration range and variation tendency, hence it is evident that facilitate clinical diagnosis and treatment detection use.The present invention may be used also
It, can tumor markers in easy, quick test sample using test equipment.
Further, the tumor markers CYFRA 21-1 of immunoassay strip detection non-small cell lung cancer of the present invention,
The result shows that the sensitivity of the immunoassay strip is 5ng/ml;Immunoassay strip detection Small Cell Lung Cancer of the present invention swells
Tumor markers NEC, the results showed that the sensitivity of the immunoassay strip is 15ng/ml;Immunoassay strip detection of the present invention
The tumor markers Pro-GRP of Small Cell Lung Cancer, the results showed that the sensitivity of the immunoassay strip is 15ng/ml.
Detailed description of the invention
Fig. 1 is that the non-small cell lung cancer tumor markers CYFRA 21-1 qualitative detection that the embodiment of the present invention 1 obtains is positive
As a result;
Fig. 2 is that the non-small cell lung cancer tumor markers CYFRA 21-1 qualitative detection that the embodiment of the present invention 1 obtains is negative
As a result;
Fig. 3 is the non-small cell lung cancer tumor markers CYFRA 21-1 quantitative detection result that the embodiment of the present invention 1 obtains;
Fig. 4 is the tumor markers NSE qualitative detection positive findings for the Small Cell Lung Cancer that the embodiment of the present invention 2 obtains;
Fig. 5 is the tumor markers NSE qualitative detection negative findings for the Small Cell Lung Cancer that the embodiment of the present invention 2 obtains;
Fig. 6 is the tumor markers NSE quantitative detection result for the Small Cell Lung Cancer that the embodiment of the present invention 2 obtains;
Fig. 7 is the Small Cell Lung Cancer pro-GRP qualitative detection positive findings that the embodiment of the present invention 3 obtains;
Fig. 8 is the Small Cell Lung Cancer pro-GRP qualitative detection negative findings that the embodiment of the present invention 3 obtains;
Fig. 9 is the Small Cell Lung Cancer pro-GRP quantitative detection result that the embodiment of the present invention 3 obtains;
Figure 10 is the obtained bis- lung cancer tumor markers of CYFRA 21-1 and pro-GRP of the embodiment of the present invention 4 same
Qualitative detection result on a detector bar.
Specific embodiment
The present invention provides the immunochromatography detector bars of a kind of quick diagnosis and monitoring lung cancer, on the detector bar from it is lower to
Last time is disposed with sample uptake zone, solid phase labelling antibody district, reaction zone and suction zones, and the reaction zone is equipped with detection line T
Nature controlling line C, the detection line T are provided with one or two, the nature controlling line C setting one or more;The detection line packet
The corresponding antibody of tumor markers for being had detection Small Cell Lung Cancer and/or non-small cell lung cancer;The solid phase labelling antibody district
There is the corresponding labelled antibody of tumor markers of detection Small Cell Lung Cancer and/or non-small cell lung cancer.
Using the tumor markers of double antibody sandwich method principle detection lung cancer, the immunoassay strip has to be detected the present invention
As a result the characteristics of accurate, high sensitivity, and it is easy to carry and save;Easy to operate, method is stable, can individually test, and 10
Minute completes test.Have the characteristics that can quantitative and semi-quantitative measurement, can reflect the tumor markers of lung cancer concentration range and
Variation tendency, hence it is evident that facilitate the use of clinical diagnosis and treatment detection.Test equipment also can be used in the present invention, can quickly detect
Tumor markers in sample.
Immunoassay strip provided by the invention includes bottom plate.The present invention does not have the material, size and source of the bottom plate
Special limitation, using the bottom plate well known to those skilled in the art in immunoassay strip.In the present invention, described
The material of bottom plate is preferably plastics or film condensation material.Bottom plate used in the embodiment of the present invention is PVC board, is commercial goods.
Immunoassay strip provided by the invention includes the sample uptake zone being arranged on the bottom plate.In the present invention, institute
The material for stating sample uptake zone is preferably glass fibre.The present invention does not have any restrictions, ability to the source of the glass fibre
Glass fibre known to field technique personnel.The present invention pastes in the lower end of bottom plate the sample uptake zone, will be described
The lower edge of sample uptake zone is concordant with bottom plate lower edge.
In the present invention, the sample that the sample uptake zone absorbs is blood, serum or blood plasma.The source of the blood does not have
Limitation, using blood well-known to those skilled in the art or serum, blood plasma.The sample added in the embodiment of the present invention
It can be finger blood.
Immunoassay strip provided by the invention includes the solid phase labelling antibody district being arranged on bottom plate, and the solid phase labelling is anti-
Body area is located above sample uptake zone, and the overlapping 1.5mm of the solid phase labelling antibody district and sample uptake zone.In the present invention
In, the material of the solid phase labelling antibody district is preferably glass fibre.
Immunoassay strip provided by the invention includes the reaction zone being arranged on the bottom plate, and the reaction zone is close to described
Solid phase labelling antibody district, positioned at the top of the solid phase labelling antibody district, and the reaction zone and the solid phase labelling antibody
Area overlapping 1.5mm.In the present invention, the material of the reaction zone is preferably nitrocellulose filter.
In the present invention, the reaction zone is provided with detection line T and nature controlling line C.The detection line T is located at nature controlling line C's
In the following, the detection line T is close to solid phase labelling antibody district, the nature controlling line C is close to water suction section.
The present invention is one or two to the item number of the detection line T, when the detection line T is one, the detection
Line T is coated with the corresponding antibody of tumor markers of detection Small Cell Lung Cancer or non-small cell lung cancer;When the detection line is two
When, two detection line T are coated with antibody corresponding to the tumor markers of Small Cell Lung Cancer and non-small cell lung cancer respectively.Institute
It closes the upper and lower position for stating antibody corresponding to the tumor markers of two detection line T coating Small Cell Lung Cancer and non-small cell lung cancer
System is not particularly limited.In the present invention, the peridium concentration of the antibody is preferably 2~3mg/ml.
In the present invention, the tumor markers of the Small Cell Lung Cancer are preferably NEC, gastrin-releasing peptide precursor pro-
GRP。
In the present invention, the tumor markers antigen of the non-small cell lung cancer is preferably cyokeratin antigens c YFRA 21-
1, phosphorus shape cellular antigens SCC or value of tissue polypeptide specific antigen TPS.
In the embodiment of the present invention, an antibody sources of the corresponding antibody of the tumor markers and labelled antibody are all from
JAJ international,Inc.San Diego,CA,USA.The tumour of non-small cell lung cancer described in the embodiment of the present invention
The product type of the coated antibody of marker CYFRA 21-1 is #CY-0402, the tumor markers CYFRA of non-small cell lung cancer
The product type of the labelled antibody of 21-1 is #CY-0128;The coated antibody of the tumor markers NEC of the Small Cell Lung Cancer
Product type is #EC-0222, and the labelled antibody product type of the tumor markers NEC of the Small Cell Lung Cancer is #NEC-
0128;The coated antibody product type of the tumor markers Pro GRP of the Small Cell Lung Cancer is #GRP-0512, described
The labelled antibody product type of the tumor markers Pro GRP of Small Cell Lung Cancer is #GRP-0518.
In the present invention, the colour developing situation of the detection line T detects by an unaided eye or is determined according to the data that reader measures
Testing result is measured, or half-quantitative detection result is obtained to compare with the intensity of check plot nature controlling line C.
Immunoassay strip provided by the invention includes the suction zones being arranged on the bottom plate, and the suction zones are close to reaction
The top of the reaction zone is arranged in area, and the reaction zone is concordant with the top edge of bottom plate.The present invention is to the blotting paper
Source there is no any restrictions, blotting paper well-known to those skilled in the art.
The present invention provides immunoassay strip, and it is also preferable to include the plastic housings for containing detector bar.In the present invention, the plastics
Shell includes bottom cover and the top cover with bottom cover cooperation, and the bottom cover has width groove corresponding with detector bar, the length of detector bar
The size of degree and width and groove is coincide, and hole identical with reaction zone and sample addition area's size is provided on the top cover,
So that operator observes testing result.
The preparation method of immunoassay strip described in above-mentioned technical proposal provided by the invention, including the following steps:
1) labelled antibody is dissolved in buffer, the diluted labelled antibody is sprayed on immobilon-p, e.g., glass
Then fibrous paper is dried in vacuum desiccator;
2) antibody-solutions are lined into formation detection line T line on reaction zone, while rabbit anti-mouse igg solution is crossed and being reacted
Nature controlling line C line is formed in area, and the reaction zone of scribing line is placed under 25~37 DEG C of environment and is dried 10~20 minutes;
3) sample uptake zone, solid phase labelling antibody district, chromatographic film blotting paper are fixed on bottom plate, are cut into item, obtained
Immunoassay strip;
The present invention is not particularly limited the label " antibody gold particle ", using mark well-known to those skilled in the art
Remember " antibody gold particle " liquid.
One antibody-solutions are lined into formation detection line T line on reaction zone, rabbit anti-mouse igg solution is lined on reaction zone
Form nature controlling line C line, the reaction zone crossed.The present invention is not restricted to the mode of the scribing line, using this field skill
Labelled antibody spraying method known to art personnel.Scribing line is crossed using lining instrument in the embodiment of the present invention, and described stroke
Model the Biodot ZX-1000CA, USA of line instrument.In the present invention, the concentration of the coating buffer is preferably 2mg/ml;It is described
Coating buffer quantity for spray is preferably that every 20cm nitrocellulose filter sprays 80ul/ seconds
After the reaction zone crossed, the reaction zone of scribing line is placed under 25~37 DEG C of environment and dries 10~20 points by the present invention
Clock, the reaction zone dried.The present invention is not particularly limited the method for the drying, ripe using those skilled in the art institute
The furnace drying method known.
After obtaining solid phase labelling antibody district and reaction zone, the present invention is by sample uptake zone, solid phase labelling antibody district, reaction zone
It is fixed on bottom plate after being cut with suction zones, the bottom plate assembled.
The present invention is preferably first from bottom to top successively to paste sample uptake zone, solid phase labelling antibody in bottom plate to the assembling
Area, reaction zone, suction zones, it is described to paste the overlapping 1.5~2mm for making each area.
After the bottom plate assembled, the present invention cuts the bottom plate of assembling, obtains immunoassay strip.The present invention is to institute
The method for stating slitting is not particularly limited, using slitting method well-known to those skilled in the art.The embodiment of the present invention
Middle slitting is cut using cutting machine, cutting machine the model Kinemitic2360CA, USA.
In the present invention, the width of the slitting is preferably 2.5~5mm, more preferably 4mm.
Below with reference to embodiment to a kind of quick diagnosis provided by the invention and monitor lung cancer immunochromatography detector bar and
Preparation method is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Colloidal gold solution is added in the antibody that product type is #CY-0128, to the labelled antibody-colloidal gold without spy
Different limitation, using labelled antibody colloidal gold liquid well-known to those skilled in the art,
The diluted labelled antibody is impregnated into immobilon-p, the glass fibre such as all-glass paper 10 minutes, after immersion
In vacuum environment, 37 DEG C are dried film.The phosphate buffer that is dissolved in for being #CY-0402 by product type, forms coating buffer, will
Concentration is that the coating buffer of 2mg/ml lines on reaction zone formation detection line T, while rabbit anti-mouse igg solution being crossed and reaction zone
Upper formation nature controlling line C line, makes T line below C line, and the reaction zone of scribing line is placed under 37 DEG C of environment and is dried 15 minutes.By sample
Product uptake zone, solid phase labelling antibody district, reaction zone are fixed on bottom plate after being cut, and blotting paper is sticked in upper end again, is cut into
Item obtains immunoassay strip.
Preparatory gradient dilution standard antigen tumor markers CYFRA 21-1 concentration (ng/ml) to be checked be 0,2.5,5,10,
25,50, it drips the diluted liquid to be checked of 2-3 drop respectively on immunoassay strip, while using phosphate buffer as negative control, takes out
Immunoassay strip is laid flat on desktop, observation chromatographic solution migration, after ten minutes the result in observing response area.
Testing result detects by an unaided eye, and the liquid testing result of positive lung cancer is as shown in Figure 1, work as tumor markers CYFRA
T line and C line develop the color simultaneously when the concentration of 21-1 is greater than 5ng/ml, when the concentration of tumor markers CYFRA 21-1 is less than 5ng/ml
When there was only the colour developing of C line, and T line does not develop the color, it can thus be seen that the tumor markers of immune lung cancer detection item provided by the invention
The sensitivity of CYFRA 21-1 is 5ng/ml.
It will test result and be placed on QicKTech4000 on readout instrument (from JAJ international, Inc.San
Diego, CA, USA) under detected, testing result is as shown in table 1.Standard curve, which is drawn, according to 1 result data of table obtains Fig. 3,
Middle horizontal axis indicates the concentration (unit ng/ml) of measuring samples, and the longitudinal axis indicates color intensity.By equation y=- is calculated
0.00038x2+ 0.07088x+0.07792, R2=1.As can be seen that the invention immunoassay strip detects tumor markers
The quantitative detection result linearly dependent coefficient of CYFRA 21-1 is 1, shows that detected value and theoretical value can preferably coincide, detection knot
Fruit is accurately credible.
The tumor markers CYFRA 21-1 quantitative detection tables of data that 1 embodiment of the present invention 1 of table obtains
Embodiment 2
Colloidal gold solution is added in the antibody that product type is #NEC-0128, to the labelled antibody-colloidal gold without spy
Different limitation, using labelled antibody colloidal gold liquid well-known to those skilled in the art.It is #EC-0222's by product type
Antibody is dissolved in phosphate buffer, forms coating buffer, and the coating buffer that concentration is 2mg/ml is lined formation detection line T on reaction zone
Line, while nature controlling line C line will be formed on the scribing line of rabbit anti-mouse igg solution and reaction zone, make T line below C line, by the glass of scribing line
Glass tunica fibrosa is placed under 37 DEG C of environment and dries 15 minutes.By related component, cut after be fixed on bottom plate, upper end is sticked again
Blotting paper is cut into item, obtains immunoassay strip.Preparatory gradient dilution measuring samples, make the marker NSE concentration (ng/ml) be
0,5,15,40,100,200, it is dripped respectively on immunoassay strip in the diluted liquid to be checked of 2-3 drop, while being made with phosphate buffer
It for negative control, takes out immunoassay strip and lays flat on desktop, observation chromatographic solution migrates, after ten minutes the result in observing response area.
Testing result detects by an unaided eye, and the liquid testing result of positive lung cancer is as shown in figure 4, work as the dense of tumor markers NSE
T line and C line develop the color simultaneously when degree is greater than 15ng/ml, only have C line aobvious when the concentration of tumor markers NSE is less than 15ng/ml
Color, and T line does not develop the color, testing result is as shown in figure 5, only C line develops the color, and T line does not develop the color.It can thus be seen that of the invention
The sensitivity of the tumor markers NSE of immune lung cancer detection item is 15ng/ml.
It will test result and be placed under readout instrument and detected, testing result is as shown in table 2.It is drawn according to 2 result data of table
Standard curve processed obtains Fig. 6, and wherein horizontal axis indicates the concentration (unit ng/ml) of measuring samples, and the longitudinal axis indicates color intensity.By meter
Calculation obtains equation y=-0.00038x2+ 0.07088x+0.07792, R2=1.As can be seen that the invention immunoassay strip detection
The quantitative detection result linearly dependent coefficient of tumor markers NSE is 1, shows that detected value and theoretical value can preferably coincide, and is examined
It is accurately credible to survey result.
Claims (8)
1. a kind of quick diagnosis and monitoring lung cancer immunochromatography detector bar, which is characterized in that including bottom plate, on the bottom plate by
Be followed successively by sample uptake zone, solid phase labelling antibody district, reaction zone and suction zones upwards down, the reaction zone be equipped with detection line T and
Nature controlling line C, the detection line T are provided with one, and the nature controlling line C is arranged one;The detection line is coated with detection cellule
The corresponding antibody of the tumor markers of lung cancer and/or non-small cell lung cancer;The solid phase labelling antibody district has detection cellule lung
The corresponding labelled antibody of the tumor markers of cancer;
The tumor markers of the Small Cell Lung Cancer are gastrin-releasing peptide precursor Pro-GRP;The immunochromatography detector bar
Detection sensitivity is 15ng/ml;
The tumor markers NSCLC antigen of the non-small cell lung cancer is cyokeratin antigens c YFRA21-1;The immunochromatography
The detection sensitivity of detector bar is 5ng/ml.
2. immunochromatography detector bar according to claim 1, which is characterized in that test object blood samples uptake zone S is added,
By or do not push lung cancer specific antigen or tumour mark of the blood sample liquid in chromatographic film uplink, blood sample by a kind of buffer
Will object first forms " labelled antibody --- LuCA " immune complex with the labelled antibody of the solid phase labelling antibody district, via
Chromatographic theory continues uplink along immunochromatography item, adsorbs in the immobilised pairing specific antibody of the detection zone T and detection zone T,
Form " labelled antibody --- LuCA --- immobilized antibody " sediment;The concentration and LuCA of the sediment are in blood sample
Concentration it is in direct ratio.
3. immunochromatography detector bar according to claim 1, which is characterized in that the marker of the labelled antibody is gold
Grain, enzyme, light or carbon granules.
4. immunochromatography detector bar according to claim 1, which is characterized in that coated tumor markers are corresponding in detection line
Antibody peridium concentration be 1~3mg/ml.
5. the colour developing situation of immunochromatography detector bar according to claim 1, the detection line T detects by an unaided eye or according to reading
Number devices measurement data obtain quantitative detection as a result, or made comparisons with the intensity of check plot C, obtain half-quantitative detection result.
6. immunochromatography detector bar according to claim 1, the immunoassay strip further includes the plastic housing for containing detector bar.
7. immunochromatography detector bar according to claim 1, which is characterized in that sample to be tested is blood, serum or blood plasma.
8. immunochromatography detector bar according to claim 1, lung cancer detection item merges use with other Tumor invasions.
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