CN102221612A - Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen - Google Patents
Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen Download PDFInfo
- Publication number
- CN102221612A CN102221612A CN2011101544432A CN201110154443A CN102221612A CN 102221612 A CN102221612 A CN 102221612A CN 2011101544432 A CN2011101544432 A CN 2011101544432A CN 201110154443 A CN201110154443 A CN 201110154443A CN 102221612 A CN102221612 A CN 102221612A
- Authority
- CN
- China
- Prior art keywords
- line
- cea
- cancer
- detection
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a detection method in the technical field of biological engineering and relates to a method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen in particular. The carcinoembryonic antigen is a broad-spectrum tumor marker, can reflect the existences of multi-tumors to people and is a better tumor marker for curative effect judgment, disease development, monitoring and preoperative prognosis on colorectal cancer, breast cancer and lung cancer. Clinic detection on CEA (carcinoembryonic antigen) is usually served as a detection criterion for the early aided diagnosis and cancer postoperation recrudescence of colon cancer, gastric cancer, esophagus cancer, rectal cancer, etc. At present, common CEA detection methods on clinic detection comprise ELISA (enzyme-linked immuno sorbent assay), CLIA and so on, waste time and labor, is not beneficial to popularization and has high cost. The technology of immune collaurum develops rapidly and is applied widely to the detection on infectious diseases, early pregnancy, cancer and the like. The method adopts the collaurum immune chromatography to build a method for quickly detecting the CEA, and can detect the range of a CEA gray area from 5ng/mL-20ng/mL with semi quantity. The CEA can be taken as the basis for differential diagnosis of benign and malignant tumors.
Description
Technical field
The present invention relates to a kind of detection method of technical field of bioengineering, specifically is the method for the two index line immunochromatography sxemiquantitative diagnosis of a kind of usefulness carcinomebryonic antigen.
Background technology
Malignant tumour has become the principal disease of harm humans health, and wherein the morbidity height of alimentary system malignant tumour ranks first.Show according to domestic relevant clinical investigation, alimentary system malignant tumour incidences of disease in the crowd such as colorectal cancer, colon cancer, liver cancer and cancer of pancreas rise to some extent in recent years, alimentary system malignant tumour accounts for 60～70% of all tumor invasions, rank first place, on mortality ratio " ranking list ", also be " coming out at the top ", and be rejuvenation trend, the importance of therefore carrying out digestive system tumor diagnoses and treatment research is self-evident.This shows that following China is for high-quality digestive system tumor diagnostic products, and especially the demand of CEA diagnostic products is with increasing.
Colorectal Carcinoma can produce a kind of glycoprotein, causes patient's immune response as antigen.This kind antigen is called carcinomebryonic antigen (carcinoembryonic antigen CEA), can extensively be present in the digestive system cancer of endoderm origin.Also have on a small quantity synthetic in adult's intestines and stomach, but do not enter hematological system but discharge by intestines and stomach, therefore, have only the carcinomebryonic antigen of trace in the normal adult human serum, concentration is less than 5ng/mL.When gastroenteric tumor, the carcinomebryonic antigen in the serum can obviously raise, if the carcinomebryonic antigen in the serum surpasses every liter of blood 20 μ g, prompting has gastroenteric tumor.But other malignant tumour such as lung cancer, cancer of pancreas, breast cancer and some benign diseases such as nonspecific colonitis, collagenosis, angiocardiopathy etc. also can raise.
Carcinomebryonic antigen is a broad spectrum activity tumor markers, and it can reflect the existence of kinds of tumors to people, curative effect judgement, PD, monitoring and the prognosis of colorectal cancer, breast cancer and lung cancer are estimated, and be a tumor markers preferably.The detection index that clinically early stage auxiliary diagnosis such as the detection Chang Zuowei colon cancer of CEA, cancer of the stomach, the carcinoma of the rectum, cancer of the esophagus and cancer postoperative is had or not recurrence.Benign tumour, inflammation and degenerative disease are as polyp of colon, ulcerative colitis, pancreatitis and alcoholic cirrhosis patient CEA also have part to raise, but well below malignant tumour, be generally less than 20ng/mL, CEA has often pointed out tumor in digestive tract when surpassing 20ng/mL.So measuring CEA can be used as optimum and antidiastole foundation malignant tumour.
Some tumours such as early carcinoma of stomach 70% above non-evident sympton have brought difficulty to early diagnosis, in case occurred manifest symptom clinically, have often belonged to the late period of pathology.Therefore, early detection is also in time gone to a doctor and is become the focal issue that improves cure rate, and at present, concentrates on this specific proteins of carcinomebryonic antigen CEA for the early detection of digestive system tumor.Higher CEA level means that perhaps cancer can exist, and at this moment just need carry out biopsy and confirm diagnosis.At present, the method that CEA commonly used clinically detects is ELISA, methods such as CLIA.Said method is time-consuming, require great effort, be unfavorable for to popularize and expense expensive.Exploitation simply, the carcinomebryonic antigen diagnostic method has crucial economic worth and social effect fast.
Immune colloidal gold technique is to be firstly appeared by Faulk and Taylor phase early 1970s, is used for immunoelectronmicroscopy at first.Colloidal gold-labeled method is as tracer label thing or developer with collaurum, in a kind of novel immunolabelling technique of antigen-antibody reaction, successfully be used for the fields such as manufacturing of Electronic Speculum, flow cytometer, Western blotting, protein staining, in-vitro diagnosis preparation now.Present golden labelling technique often cooperates with membrane carrier, forms specific immune detection mode, as immunity percolation test and immunity-chromatography test etc.Immuno-chromatographic test paper strip is exactly the important development direction that this technology is used for external quick diagnosis, is the novel detection technique that grows up on modern monoclonal antibody technique, colloidal gold immunochromatographimethod technology and new material technology basis.This technical development in recent years is rapid, has particularly obtained widespread use in the bedside detection (POCT) in clinical diagnosis, as the detection of infectious disease, early pregnancy, cancer etc.
Utilize the detection method of collaurum to measure CEA in the serum and have or not the detection index of recurrence as early stage auxiliary diagnosis such as colon cancer, cancer of the stomach, the carcinoma of the rectum, cancer of the esophagus and cancer postoperative, be developed, it is limited to 5ng/mL under detecting.But for use separately clinically this standard of 5ng/mL can not be directly, distinguishing benign and malignant tumour fast, also be very necessary so exploitation can detect the sxemiquantitative colloid gold test paper of this CEA diagnosis gray area of CEA 5～20ng/mL.All not having relevant test strips at present both at home and abroad sells.
Summary of the invention
Purpose of the present invention: exploitation simply, the carcinomebryonic antigen diagnostic method has crucial economic worth and social value fast.Colloidal gold immunity chromatography is adopted in this experiment, sets up the method for fast detecting CEA.And can this CEA gray area scope of half-quantitative detection 5ng/mL～20ng/mL.
The present invention is achieved by the following technical solutions: adopt trisodium citrate reduction method to prepare colloid gold particle, the anti-CEA monoclonal antibody 1 of mark is also evenly coated on the glue gold pad, as the trace labelling thing.On nitrocellulose filter, the CEA monoclonal antibody 2 of two variable concentrations is fixed in two detection lines (being the T line), the sheep anti mouse two anti-nature controlling lines (being the C line) that are fixed in.Successively with sample pad, glue gold pad, nitrocellulose filter and absorbent filter assembling, be cut into colloid gold test paper and the test card of packing in.The gold labeling antibody combines with corresponding antigen, antibody generation specificity and is trapped and develops the color, and whether develops the color according to T line, C line and judges the yin and yang attribute result.
The inventive method comprises the steps:
One, colloidal gold solution preparation
1. prepare 1% chlorauric acid solution;
2. prepare 2% citric acid three sodium solution;
3. 0.02% chlorauric acid solution is heated to boiling, adds 2% trisodium citrate 2mL rapidly;
4. solution is by light blue, and dark blue, claret appears in heating again, continues to boil 10min;
Stop heating, continue to be stirred to room temperature.
Two, the preparation of colloid gold label thing
1. get two 1.5mL test tubes, add the 1mL colloidal gold solution respectively;
2. add an amount of borate buffer solution pH is adjusted into 8.8;
3. add 20 μ g/mL CEA monoclonal antibodies 1, make it reach minimum protein concentration, mixing, vibration 30min;
4. the 10%BSA that adds 20 μ L, mixing, vibration 30min;
5.12000rpm centrifugal 5min absorbs supernatant gently;
6. with the loose collaurum precipitation of 1mL wash buffer solution weight suspension;
7. repeat the operation of 5. steps;
8. repeat the operation of 6. steps;
9. repeat the operation of 5. steps;
10. being formulated in the OD of 540nm place (optical density) value with fix buffer is 0.5～1.0 colloid gold immune compound;
11. the plain film spot sample of glass fibre forms glue gold pad, vacuum freeze-drying 3 hours.
Three, on nitrocellulose filter, rule
1.T1 line concentration is CEA monoclonal antibody 2 line of 0.2mg/mL, is used to detect the CEA of 5ng/mL;
2.T2 line concentration is CEA monoclonal antibody 2 line of 0.1mg/mL, is used to detect the CEA of 20ng/mL;
3.C line is rule with 3.5mg/mL sheep anti mouse polyclonal antibody;
4. nitrocellulose filter was placed 40 ℃ of oven dryings 3 days.
Four, the assembling of colloidal gold strip
1. sample pad, glue gold pad, NC film and absorption pad 4 parts are assembled (seeing accompanying drawing 1) in order;
2. it is assembled and detect after the back is cut into the 4mm/ bar with scissors.
Five, detect
Test paper keeps flat, and sample is added on the sample pad of detector bar, because capillary effect, the chromatography direction of liquid combines and is trapped and develops the color with material on being fixed on T line, C line forward.Behind the 5min, judge the yin and yang attribute result according to the colour developing situation of T line, C line.The C line with take out of near the detection of C line existing redness be in the sample CEA level greater than 5ng/mL (seeing accompanying drawing 2), C line and two detect band all occurs red be in the sample CEA level greater than 20ng/mL (seeing accompanying drawing 3), have only the C line red negative (seeing accompanying drawing 4) to occur, T line and C line all do not develop the color, and then test strips is invalid.
Detected object of the present invention is single and with strong points, the accuracy rate height.Detection speed is fast, and required time is short, only needs 5min, does not need just can use the inventive method to detect through the professional of training, satisfies departments such as hospital, outpatient service and diagnoses the requirement of digestive system tumor quickly and accurately, and be convenient to basic unit's popularization and utilization.
Description of drawings
Fig. 1 is the diagram of embodiment of the invention test strips
Fig. 2 is the positive findings (the CEA level is greater than 5ng/mL) of embodiment of the invention test strips
Fig. 3 is the positive findings (the CEA level is greater than 20ng/mL) of embodiment of the invention test strips
Fig. 4 is the negative findings of embodiment of the invention test strips
Embodiment
Below in conjunction with accompanying drawing embodiments of the invention are elaborated: present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Among the following embodiment, the experimental technique of unreceipted actual conditions, usually according to normal condition, or the condition of advising according to manufacturer.
Jinsui River that this example prepares 20nm with 2% citric acid three sodium solution earlier, and mark CEA monoclonal antibody 1, down payment mark monoclonal antibody, CEA monoclonal antibody 2, sheep anti mouse polyclonal antibody metal spraying mark pad, detection line (T line), nature controlling line (C line), be assembled into test strips then, put 37 ℃ of oven for drying and be cut into the 4mm/ bar, drying at room temperature is preserved standby.
Embodiment
1. prepare CEA monoclonal antibody 1 and 2.And the required glassware of cleaning experiment.
2. the preparation of collaurum
With 0.02% chlorauric acid solution solution heated and boiled, add 2% trisodium citrate 2mL rapidly earlier;
Solution colour becomes dark bluely by light blue, continues heating and claret occurs, continues to boil 10min; Transparent claret occurs, stop heating, continue to be stirred to room temperature.The colloidal gold solution that has so just prepared 20nm.Use the Electronic Speculum microscopy then, guarantee that the gold grain for preparing makes its big or small consistent and uniform as far as possible, particle diameter is about 20nm, otherwise making again.
3. the preparation of colloid gold label thing
Get two 1.5mL test tubes, add the 1mL colloidal gold solution respectively; To wherein adding an amount of borate buffer solution pH is adjusted into 8.8; Add 20 μ g/mL CEA monoclonal antibodies 1, make it reach minimum protein concentration, mixing, quick oscillation 30min on shaker; The 10%BSA that adds 20 μ L, mixing, vibration 30min; 12000rpm in hydro-extractor, centrifugal 5min absorbs supernatant gently; With the loose collaurum precipitation of 1mL wash buffer solution weight suspension; 12000rpm in hydro-extractor, centrifugal 5min absorbs supernatant gently; With the loose collaurum precipitation of 1mL wash buffer solution weight suspension; 12000rpm in hydro-extractor, centrifugal 5min absorbs supernatant gently; Being formulated in the OD of 540nm place (optical density) value with fix buffer is 0.5～1.0 colloid gold immune compound; Point sample on the plain film of glass fibre forms glue gold pad, vacuum freeze-drying 3 hours.
4. the assembling of colloidal gold strip
On 2 strokes of detection lines of CEA monoclonal antibody (T line) with 0.2mg/mL and 0.1mg/mL, 3.5mg/mL sheep anti mouse polyclonal antibody is drawn on nature controlling line (C line) respectively at nitrocellulose filter.Then nitrocellulose filter was placed 40 ℃ of dryings of baking oven 3 days.Then various piece is assembled into test strips by accompanying drawing 1.
5. the use of colloidal gold strip and interpretation as a result
Before the detection, extract examinee's serum sample earlier.
Then the test strips test paper is kept flat, sample is added on the sample pad of detector bar, because capillary effect, the chromatography direction of liquid combines and is trapped and develops the color with material on being fixed on T line, C line forward.Behind the 5min, judge the yin and yang attribute result according to the colour developing situation of T line, C line.The C line with take out of near the detection of C line existing redness be in the sample CEA level greater than 5ng/mL (seeing accompanying drawing 2), C line and two detect band all occurs red be in the sample CEA level greater than 20ng/mL (seeing accompanying drawing 3), have only the C line red negative (seeing accompanying drawing 4) to occur, T line and C line all do not develop the color, and then test strips is invalid.
Embodiment is the CEA in the test sample directly, and using method does not need professional training, and is easy to operate, quick, and 5min can obtain the result, can reach quick, easy, detect the purpose of CEA in time.
Claims (3)
1. the method with two index line immunochromatography sxemiquantitative diagnosis carcinomebryonic antigens is characterized in that, adopts trisodium citrate reduction method to prepare colloid gold particle, and the anti-CEA monoclonal antibody 1 of mark is also evenly coated on the collaurum pad, as the trace labelling thing.On nitrocellulose filter, the CEA monoclonal antibody 2 of two variable concentrations is fixed in two detection lines (being the T line), the sheep anti mouse two anti-nature controlling lines (being the C line) that are fixed in.Successively with sample pad, glue gold pad, nitrocellulose filter and absorbent filter assembling, be cut into colloid gold test paper and the test card of packing in.The gold labeling antibody combines with corresponding antigen, antibody generation specificity and is trapped and develops the color, and whether develops the color according to T line, C line and judges the yin and yang attribute result.
2. the method for the two index line immunochromatography sxemiquantitative diagnosis of a kind of usefulness according to claim 1 carcinomebryonic antigen is characterized in that antibody line concentration is:
T1 line concentration is CEA monoclonal antibody 2 line of 0.2mg/mL, is used to detect the CEA of 5ng/mL;
T2 line concentration is CEA monoclonal antibody 2 line of 0.1mg/mL, is used to detect the CEA of 20ng/mL;
The C line is rule with 3.5mg/mL sheep anti mouse polyclone carrier;
Nitrocellulose filter was placed 40 ℃ of oven dryings 3 days.
3. the method for the two index line immunochromatography sxemiquantitative diagnosis of a kind of usefulness according to claim 1 carcinomebryonic antigen, it is characterized in that, the antibody-solutions of utilization variable concentrations is rule on identical matrix, detects the method for the horizontal material of a plurality of variable concentrations with the collaurum method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101544432A CN102221612A (en) | 2011-06-10 | 2011-06-10 | Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101544432A CN102221612A (en) | 2011-06-10 | 2011-06-10 | Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102221612A true CN102221612A (en) | 2011-10-19 |
Family
ID=44778219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101544432A Pending CN102221612A (en) | 2011-06-10 | 2011-06-10 | Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102221612A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539783A (en) * | 2011-12-22 | 2012-07-04 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitatively diagnosing myoglobin with double-index-line immunochromatography |
| CN102539769A (en) * | 2011-12-22 | 2012-07-04 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography |
| CN102565417A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing cardiac troponin I (cTn I) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN102565407A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitative diagnosis of clenbuterol hydrochloride through double-indicating line immunochromatography |
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN102565384A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing ractopamine in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN103217532A (en) * | 2012-01-18 | 2013-07-24 | 北京永瀚星港生物科技股份有限公司 | C-reactive protein detection test paper strip |
| CN106018807A (en) * | 2016-05-11 | 2016-10-12 | 卢氏实验室公司 | Immunochromatographic assay strip for rapidly diagnosing and monitoring lung cancer and preparation method of assay strip |
| CN106526201A (en) * | 2016-11-10 | 2017-03-22 | 陕西师范大学 | Method for detecting antigens in qualitative and semi-quantitative mode based on paper chip immunoreaction distance |
| CN106841621A (en) * | 2017-04-06 | 2017-06-13 | 黑龙江省科学院高技术研究院 | Tumor markers CEA quick detection test papers |
| CN116718775A (en) * | 2023-08-04 | 2023-09-08 | 天津迈基生物科技有限公司 | Composition, test paper and method for detecting colorectal cancer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260490A (en) * | 1998-12-30 | 2000-07-19 | 中国科学院上海药物研究所 | Semi-quantitative one-step immunologic diagnosis method |
| CN101261271A (en) * | 2008-04-17 | 2008-09-10 | 武汉工业学院 | Sudan red 1 immunity-chromatography test paper detection method |
| CN101609096A (en) * | 2009-07-21 | 2009-12-23 | 上海师范大学 | Lung cancer marker detection immunochromatographitest test paper and application |
| CN101750491A (en) * | 2008-12-16 | 2010-06-23 | 陈洁 | Cancer saliva rapid detection reagent |
-
2011
- 2011-06-10 CN CN2011101544432A patent/CN102221612A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260490A (en) * | 1998-12-30 | 2000-07-19 | 中国科学院上海药物研究所 | Semi-quantitative one-step immunologic diagnosis method |
| CN101261271A (en) * | 2008-04-17 | 2008-09-10 | 武汉工业学院 | Sudan red 1 immunity-chromatography test paper detection method |
| CN101750491A (en) * | 2008-12-16 | 2010-06-23 | 陈洁 | Cancer saliva rapid detection reagent |
| CN101609096A (en) * | 2009-07-21 | 2009-12-23 | 上海师范大学 | Lung cancer marker detection immunochromatographitest test paper and application |
Non-Patent Citations (1)
| Title |
|---|
| 王玉金等: "胶体金免疫层析法检测血清癌胚抗原", 《标记免疫分析与临床》, vol. 10, no. 3, 30 September 2003 (2003-09-30) * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539783A (en) * | 2011-12-22 | 2012-07-04 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitatively diagnosing myoglobin with double-index-line immunochromatography |
| CN102539769A (en) * | 2011-12-22 | 2012-07-04 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography |
| CN102565417A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing cardiac troponin I (cTn I) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN102565407A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitative diagnosis of clenbuterol hydrochloride through double-indicating line immunochromatography |
| CN102565426A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN102565384A (en) * | 2011-12-22 | 2012-07-11 | 正元盛邦(天津)生物科技有限公司 | Method for diagnosing ractopamine in semi-quantitative mode by employing double-indicatrix immunochromatography |
| CN103217532A (en) * | 2012-01-18 | 2013-07-24 | 北京永瀚星港生物科技股份有限公司 | C-reactive protein detection test paper strip |
| CN106018807A (en) * | 2016-05-11 | 2016-10-12 | 卢氏实验室公司 | Immunochromatographic assay strip for rapidly diagnosing and monitoring lung cancer and preparation method of assay strip |
| CN106526201A (en) * | 2016-11-10 | 2017-03-22 | 陕西师范大学 | Method for detecting antigens in qualitative and semi-quantitative mode based on paper chip immunoreaction distance |
| CN106526201B (en) * | 2016-11-10 | 2018-03-06 | 陕西师范大学 | A kind of method based on paper chip immune response apart from qualitative half-quantitative detection antigen |
| CN106841621A (en) * | 2017-04-06 | 2017-06-13 | 黑龙江省科学院高技术研究院 | Tumor markers CEA quick detection test papers |
| CN116718775A (en) * | 2023-08-04 | 2023-09-08 | 天津迈基生物科技有限公司 | Composition, test paper and method for detecting colorectal cancer |
| CN116718775B (en) * | 2023-08-04 | 2023-10-27 | 天津迈基生物科技有限公司 | Composition, test paper and method for detecting colorectal cancer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102221612A (en) | Method for employing double indicatrix immunochromatography by semi-quantity to diagnose carcinoembryonic antigen | |
| CN104142399B (en) | A kind ofly utilize colloidal gold immunochromatographimethod technology quantitatively test strips detecting Serum Pepsinogen and its preparation method and application | |
| CN101275954B (en) | Breast cancer early warning chip for easily rapid measuring human I type thymidine kinase gene protein | |
| CN104515859A (en) | Hemoglobin, hemoglobin-haptoglobin composite and transferrin joint examination kit and preparation method and detection method thereof | |
| CN102565426A (en) | Method for diagnosing luteinizing hormone (LH) in semi-quantitative mode by employing double-indicatrix immunochromatography | |
| CN107543932A (en) | The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of calcitonin | |
| CN207516379U (en) | A kind of colloidal gold immuno-chromatography test paper strip | |
| CN201477101U (en) | Transferrin and hemoglobin combined detection board | |
| CN101762700A (en) | Magnetic immuno-chromatographic test paper strip for quantitatively detecting carcinoembryonic antigen in blood and preparation method thereof | |
| CN103439511B (en) | A kind of liquid phase chip reagent box of detection of lung cancer | |
| CN106226518A (en) | Canine distemper virus colloidal gold immunochromatographydetection detection test paper bar and preparation method thereof | |
| CN201196651Y (en) | Gold mark detection test paper for double-tumor token | |
| CN108020666A (en) | It is a kind of can simultaneous quantitative detection blood in CEA and CA19-9 magnetic immuno-chromatographic test paper strip and preparation method | |
| CN103777026B (en) | A kind of for diagnosing the kit of hepatocellular carcinoma | |
| CN101672850A (en) | Kit for distinguishing and diagnosing hepatocirrhosis and early liver cancer and application of serum clusterin in preparing same | |
| CN101344526A (en) | Fast and accurate prostatic cancer detection test paper strip and its preparation and application | |
| CN106153934B (en) | A kind of kit of efficient quantitative detection golgiosome 73 | |
| CN103018461B (en) | Kit for early diagnosis of bladder cancer and preparation method of kit | |
| CN102565417A (en) | Method for diagnosing cardiac troponin I (cTn I) in semi-quantitative mode by employing double-indicatrix immunochromatography | |
| CN103389374B (en) | Micro whole blood application of sample method detects mycoplasma pneumoniae colloidal gold kit | |
| Shen et al. | Preparation of colloidal gold immunochromatographic test strips for the diagnosis of Toxoplasma gondii | |
| CN107271692A (en) | Fluorescent microsphere for marking specific high-affinity recombinant antibody and application thereof | |
| CN102221617A (en) | Early stage prostate cancer semi-quantitative diagnosis method utilizing double indication line immunity chromatography | |
| CN105259348A (en) | Secreting type Sema 4C protein and application thereof | |
| CN102565407A (en) | Method for semi-quantitative diagnosis of clenbuterol hydrochloride through double-indicating line immunochromatography |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20111019 |