CN109402138A - Sedum lineare resistant gene of salt SLOACPS and its application - Google Patents
Sedum lineare resistant gene of salt SLOACPS and its application Download PDFInfo
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- CN109402138A CN109402138A CN201710712875.8A CN201710712875A CN109402138A CN 109402138 A CN109402138 A CN 109402138A CN 201710712875 A CN201710712875 A CN 201710712875A CN 109402138 A CN109402138 A CN 109402138A
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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Abstract
The invention discloses sedum lineare resistant gene of salt SLOACPS and its application, sedum lineare resistant gene of salt SLOACPS is nucleotide sequence shown in SEQ ID NO.1 in sequence table, it is demonstrated experimentally that sedum lineare resistant gene of salt SLOACPS enhancing arabidopsis or Tobacco Salt performance.
Description
Technical field
The present invention relates to a kind of sedum lineare (Sedum lineare) resistant gene of salt and its application, belong to molecular biology and
Field of biotechnology.
Background technique
As China human mortality increases, the decrease of cultivated land, the development and utilization of saline alkali land resource have extremely important realistic meaning.
And Genes For Plant Tolerance is saline and alkaline, the raising of Drought resistance and be suitable for saline and alkaline aerial and with higher economy and the ecological value plant
The breeding of species or strain is then to utilize the economical and effective measure in salt-soda soil.However most plants are to saline and alkaline, arid resistance to
Poor by property, can only be grown in sodium chloride content is on 0.3% soil below, this just significantly limits these plants saline and alkaline
Growth on beach.So far plant anti-salt, Drought-tolerant gene engineering research have achieved significant progress, and having more research will resist
It is integrated into target plant after salt, Drought-tolerant gene clone, to open the new way of breeding salt-tolerant plant kind.Currently,
It needs to obtain the resistant gene of salt that preferably can increase plant salt resistant character.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of sedum lineare resistant gene of salt SLOACPS.
A second object of the present invention is to provide the cloning vectors containing sedum lineare resistant gene of salt SLOACPS.
Third object of the present invention is to provide the host cells for containing above-mentioned cloning vector.
Fourth object of the present invention is to provide the expression vector containing sedum lineare resistant gene of salt SLOACPS.
Fifth object of the present invention is to provide the host cells for containing above-mentioned expression vector.
Sixth object of the present invention is to provide sedum lineare resistant gene of salt SLOACPS enhancing arabidopsis or Tobacco Salt performances
Purposes.
Technical solution of the present invention is summarized as follows:
Sedum lineare resistant gene of salt SLOACPS is nucleotide sequence shown in SEQ ID NO.1 in sequence table.
The cloning vector pJET1.2_SLOACPS of the SLOACPS of resistant gene of salt containing sedum lineare.
Host cell containing cloning vector pJET1.2_SLOACPS.
The expression vector pBI121_SLOACPS of the SLOACPS of resistant gene of salt containing sedum lineare.
Host cell containing expression vector pBI121_SLOACPS.
Sedum lineare resistant gene of salt SLOACPS enhances the purposes of arabidopsis or Tobacco Salt performance.
Advantages of the present invention:
It is demonstrated experimentally that sedum lineare resistant gene of salt SLOACPS enhancing arabidopsis or Tobacco Salt performance.
Detailed description of the invention
Fig. 1 is SLOACPS gene cloning electrophoresis schematic diagram.
Fig. 2 is that SLOACPS is inserted into schematic diagram after expression vector.
Fig. 3 is transformant Genomic PCR the selection result after pBI121_SLOACPS arabidopsis thaliana transformation.
Fig. 4 is T3 homozygote semiquantitive PCR measurement expression result after pBI121_SLOACPS arabidopsis thaliana transformation.
Fig. 5 is SLOACPS transgenic arabidopsis T3 homozygote salt resistance experiment effect photo.
Fig. 6 is transformant Genomic PCR the selection result after pBI121_SLOACPS transformation of tobacco.
Fig. 7 is semiquantitive PCR measurement expression result after pBI121_SLOACPS transformation of tobacco.
Fig. 8 is SLOACPS transgene tobacco salt resistance experiment effect photo.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Test method without specific conditions in embodiment, usually according to normal condition and item described in handbook
Part, or according to the normal condition proposed by manufacturer.
Carrier pJET1.2:Thermo, Clone JET PCR Cloning Kit#K1231
Carrier pBIl21 is purchased from Chinese plasmid vector strain cell pnca gene collection, http: //
Biovector.blog.163.com/ embodiment 1
The clone of sedum lineare 1. (Sedum lineare Thunb, abbreviation SlT) SLOACPS gene
From the sedum lineare (being derived from Pests in Tianjin Binhai New Area) that 150mM NaCl aqueous solution is handled, plant RNeasy is used
Plant Mini Kit (Transgene Code#E101-0150rxns) extracts total serum IgE, and utilizes EasyScript
Frist-Strand cDNA SynSgesis SuperMix (Transgene Code#AE301-03100rxns) reverse transcription goes out
cDNA.3 ' the end sequences that high-flux sequence (source Nuo Hezhi company carries out high-flux sequence) obtains SLOACPS gene are carried out to cDNA
Column are obtained amplification using the full length cDNA sequence that RACE technology (Takara-RACE kit) obtains SLOACPS gene
SLOACPS gene carries out sequencing analysis, and obtaining complete SLOACPS full length gene is 579bp.When constructing overexpression vector, then
Respectively in 5 ' end addition pBI121 recombination sites of specific primer, upstream 5'- ACGGGGGACTCTAGAGGATCC-3'(SEQ
ID No.3), downstream 5'-CGATCGGGGAAATTCGAGCTC-3'(SEQ ID No.4), in favor of the structure of later stage experssion carrier
It builds.The specific steps of which are as follows:
1) synthesis of the first chain of .cDNA
With reverse transcription reagent box TaKaRaRNAPCR Kit (AMV) Ver.3.0, using total serum IgE as template, Oligo (dT) is
Primer synthesizes the first chain of cDNA under the action of AMV reverse transcriptase, and reverse transcription system is as follows:
Reaction condition: 42 DEG C of 60min, 99 DEG C of 5min.
2) sedum lineare SLOACPS gene reverse transcription quality PCR amplification detects
With sedum lineare Actin gene specific primer SEQ ID No.5:5'-GAACTTACTAGCCGACTG-3', SEQ ID
No.6:5'-CCTCAAGCCTTATACGCAA-3', PCR amplification, to verify reverse transcription reaction and RNA mass.PCR reaction system
It is as follows:
Reaction condition: 94 DEG C of 3min；94 DEG C of 30s, 40 DEG C of 30s, 72 DEG C of 50s, 35cycles；72℃5min.
3) sedum lineare SLOACPS genetic fragment PCR amplification
Sequencing analysis is carried out using the SLOACPS gene that Takara RACE kit is expanded, obtains complete sedum lineare
SLOACPS full length gene is 579bp (SEQ ID No.1).The protein encoded by sedum lineare SLOACPS gene, is SEQ ID
Amino acid sequence shown in No.2.Primer software design SLOACPS gene upstream and downstream primer is utilized according to known cDNA sequence:
SEQ ID No.7:5'-ATGTCTAGGTCGAAACTACCATTC-3',
SEQ ID No.8:5'-TTATACTGTTTTCCTGTCACACC-3', PCR reaction system is as follows:
PCR reaction condition are as follows: 95 DEG C, 5min；95 DEG C, 30s, 58 DEG C, 30s, 72 DEG C, 90s, 35cycles；72 DEG C,
10min；4 DEG C, ∞.
PCR after reaction, takes 1 μ LPCR product to carry out 1.0% agarose gel electrophoresis, detects the quality of PCR product
(see Fig. 1), remaining is used as the purification and recovery of product.
4) constructs the cloning vector containing sedum lineare SLOACPS gene
Construct the carrier pJET1.2_SLOACPS containing sedum lineare SLOACPS gene
Sedum lineare SLOACPS gene target fragment after glue recovery purifying utilizes Clone JET PCR Cloning Kit
In (pJET1.2:Thermo, Clone JET PCR Cloning Kit#K1231) recombination to carrier pJET1.2, carrier is obtained
pJET1.2_SLOACPS。
Its response procedures is as follows:
24 DEG C of reaction condition, 10min static 30min on ice, 42 DEG C of heat shock 1min30s static 2min30s on ice are transferred to sense
By state cell DH5 α, 37 DEG C, 180rpm, 45min, bacterium solution is coated onto LB (addition antibiotic Amp100uM) admittedly after this EP (end of program)
In body culture medium, 37 DEG C are incubated overnight.
Be utilized respectively the upstream and downstream primer (SEQ ID No.7 and SEQ ID No.8) of target fragment to different bacterium colonies into
Row bacterium colony PCR verifying screens positive bacterium colony sequencing, obtains the host cell containing cloning vector pJET1.2_SLOACPS.
Note: pJET1.2:Thermo, Clone JET PCR Cloning Kit#K1231 carrier are purchased from invitrogen；
Escherichia coli used are DH5 α competent cell, TIANGEN, CB101-2.
5) constructs the expression vector containing sedum lineare SLOACPS gene
Construct the expression vector pBI121_SLOACPS containing sedum lineare SLOACPS gene
When constructing overexpression vector, then pBI121 recombination sites are added at 5 ' ends of specific primer and 3 ' respectively,
SEQ ID No.3:5'–ACGGGGGACTCTAGAGGATCC-3',
SEQ ID No.4:5'-CGATCGGGGAAATTCGAGCTC-3'
It obtains:
SEQ ID No.9:5'-ACGGGGGACTCTAGAGGATCCATGTCTAGGTCGAAACTACCATTC-3'
SEQ ID No.10:5'-CGATCGGGGAAATTCGAGCTCTTATACTGTTTTCCTGTCACACC-3'
The plasmid that correct pJET1.2- gene is sequenced is extracted, as template, using containing recombination site SEQ ID
No.9, SEQ ID No.10 are that primer carries out PCR amplification, and reaction system is as follows.
PCR reaction condition are as follows: 95 DEG C, 5min；95 DEG C, 30s, 58 DEG C, 30s, 72 DEG C, 90s, 35cycles；72 DEG C,
10min；4 DEG C, ∞.
PCR after reaction, takes 1 μ LPCR product to carry out 1.0% agarose gel electrophoresis, detects the quality of PCR product
(see Fig. 1), remaining is used as the purification and recovery of product.
PBI121 plasmid is extracted, Vector map (see Fig. 2) carries out double digestion linearisation to it, and program is as follows:
Reaction condition: 37 DEG C, 12h, 80 DEG C 20min inactivations.
Gene and the pBI121 plasmid of linearisation are used into Clone Express Entry One Step Cloning
Kit kit carries out recombination to construct, and response procedures are as follows:
Response procedures: 37 DEG C, 30min, on ice 5min, 42 DEG C of heat shock 1min30s static 2min30s on ice are transferred to impression
State cell DH5 α, is coated onto LB (addition antibiotic kan 50uM) solid for bacterium solution after this EP (end of program) and trains by 37 DEG C, 180r, 45min
It supports in base, 37 DEG C are incubated overnight.
The upstream and downstream primer (see SEQ ID No.9 and SEQ ID No.10) of carrier and target fragment is utilized respectively to same
A bacterium colony carries out bacterium colony PCR double verification, and screening positive bacteria drops into row sequencing (see SEQ ID No.11), contains Fo Jia to obtain
The expression vector pBI121_SLOACPS of careless resistant gene of salt SLOACPS.
Note: this step is purchased from vazyme using Clon Express Entry One Step Cloning Kit,
6) contains the recombinant vector conversion Agrobacterium competent cell of sedum lineare SLOACPS gene
Experiment agrobacterium strains used be C58 (be purchased from Chinese plasmid vector strain cell pnca gene collection,
Http:// biovector.blog.163.com/), C58 has rifampicin resistance (Rif), and helper plasmid has celebrating
Big chloramphenicol resistance (Gen).
Using electric shock Agrobacterium-mediated Transformation method, by the coli expression carrier pBI121_ containing sedum lineare SLOACPS gene
SLOACPS is transformed into Agrobacterium strain C58 (pMP90) competent cell, 28 DEG C, cultivates 36h, bacterium colony PCR selects positive colony
Bacterium colony.
Embodiment 2
1. arabidopsis thaliana transformation
(1) arabidopsis thaliana transformation.
The concrete operation step of arabidopsis thaliana transformation:
1. the activation of positive colony bacterium colony and expansion that embodiment 1 obtains are cultivated
Activation: the positive colony bacterium colony for choosing preservation is placed in 3mLYEB fluid nutrient medium that (addition antibiotic makes Gen concentration
30mg/L, addition antibiotic Rift make concentration 25mg/L and addition antibiotic Sp make concentration 50mg/L) culture 15 hours
Left and right (to OD600=0.8 or so), 180rpm, 28 DEG C.
The expansion culture of positive colony bacterium: (the addition of suitable antibiotic is added in fresh 10Ml YEB fluid nutrient medium
Antibiotic Gen concentration is 30mg/L, antibiotic Rift concentration is 25mg/L and antibiotic Sp concentration is 50mg/L), then connect
The appropriate positive colony bacterium solution of kind is cultivated into YEB fluid nutrient medium, 180rpm, in 28 DEG C of cultures to OD600=0.6.
2. converting
Supernatant is abandoned into bacterium solution centrifugation (3000rpm, 15 DEG C, 10min) afterwards, with the mass concentration of twice the taken bacterium solution of volume
Thallus (slowly operation is to guarantee thallus vigor) is resuspended for 5% aqueous sucrose solution, so that thallus is scattered, adjusts OD600=0.8.
The wildtype Arabidopsis thaliana for choosing 3-4 weeks bolting 5-7cm of culture, is inverted in the container equipped with conversion fluid, makes entire
Inflorescence is immersed in bacterium solution 15 seconds, is taken out arabidopsis and is couched in pallet, covers moisturizing, and dark treatment 12h with plastic film, make
Arabidopsis stands upright on 25 DEG C of temperature, and photoperiod 16h illumination/8h is dark, grows under the condition of culture that relative humidity is 70%, until
Seed is mature.It is put into 37 DEG C of baking ovens after seed collection to dry two weeks, in case follow-up test uses.
(2) the homozygotic screening of transgenic arabidopsis positive transformant
By the T1 collected for seed after disinfection, it is placed on 4 DEG C of refrigerator three days, then on the super-clean bench by transgenosis
Arabidopsis seed is uniformly sowed on the 1/2MS solid screening and culturing medium containing 50 μ g/mL kanamycins, in 1800Lux, light week
Phase 16h illumination/8h is dark, grows 8-10 days, and leaf is that bottle green is the T1 of transgenic arabidopsis for positive transformant.Work as T1
When growing to 3-4 piece true leaf for positive transformant plant, being transplanted to soil, (German import peat soil 422#, is purchased from
Klasmann-Deilmann GmbH, Germany, http://www.klasmann-deilmann.com) in, at 25 DEG C of temperature,
1800Lux, photoperiod 16h illumination/8h is dark, continued growth 14 days under the condition of culture that relative humidity is 70%, first makees positive
The identification (see Fig. 3) of transformant, then first the expression of its transgenosis is identified (see Fig. 4) by semiquantitive PCR, it chooses
Independent transformation strain 2 of highly expressed independent transformation strain 6 and low expression.Continued growth under the above conditions, about one
Collecting seed after half a month is T2 for transformed the seed.Repeat the above steps to obtain No. 6 and No. 2 T3 for homozygote seed.
(3) salt stress processing is carried out to transgenic arabidopsis
No. 6 T3 are planted in the soil for homozygote seed, No. 2 T3 for homozygote seed, wild arabidopsis seed respectively,
At 25 DEG C of temperature, 1800Lux, photoperiod 16h illumination/8h is dark, grows 21 days under the condition of culture that relative humidity is 70%, often
Kind of plant retains 21 plants of consistent seedling of growing way, is randomly divided into three groups of parallel laboratory tests, every group of each 7 plants of different types of plant,
Pouring processing is carried out with salinity treatment fluid (150mM NaCl aqueous solution).It pours within 3 days once, each irrigation amount is soil quality
0.5 times, to keep constant, the plant photograph after coprocessing 15 days for the treatment of fluid concentration in basin (see Fig. 5).
Embodiment 3
1. transformation of tobacco
Tobacco for positive colony bacterium conversion is NC89 (6855-2 × 6772) tissue-cultured seedling.
(1) concrete operation step of transformation of tobacco:
1. the activation of positive colony bacterium colony and expansion that embodiment 1 obtains are cultivated
Activation: the positive colony bacterium colony for choosing preservation is placed in 3mLYEB fluid nutrient medium (addition Gen, Rift, Sp antibiosis
Element, making concentration is respectively 30mg/L, 25mg/L, 50mg/L) culture 15 hours or so (to OD600=0.8 or so), 180rpm,
28℃。
The expansion culture of positive colony bacterium: (the addition of suitable antibiotic is added in fresh 10Ml YEB fluid nutrient medium
Antibiotic Gen concentration is 30mg/L, antibiotic Rift concentration is 25mg/L and antibiotic Sp concentration is 50mg/L), then connect
The appropriate positive colony bacterium solution of kind is cultivated into YEB fluid nutrient medium, 180rpm, in 28 DEG C of cultures to OD600=0.6.
2. converting
Supernatant is abandoned into bacterium solution centrifugation (3000rpm, 15 DEG C, 10min) afterwards, with the mass concentration of twice the taken bacterium solution of volume
Thallus (slowly operation is to guarantee thallus vigor) is resuspended for 5% aqueous sucrose solution, so that thallus is scattered, adjusts OD600=0.8.
The tobacco tissue-cultured seedling for choosing growth 30 days in order, selects thick and solid blade, subtracts limb edge, blade is cut into
The explant fragment of 1cm × 1cm.The blade material sheared is immersed to the sucrose water of the mass concentration 5% containing Agrobacterium thallus
In solution, shake culture 10min under the conditions of 24 DEG C.After infecting, be placed on after explant is blotted on MS culture medium (MS salt 4.4g,
Sucrose 30g is settled to 1L, pH=5.7, agar 7.2g), dark culturing 3 days.By the explant Jing Guo dark culture with sterilized
Distilled water cleans 2-3 times, and after being blotted with filter paper, explant is placed on MS salt 4.4g on Selective agar medium, sucrose 30g, auxin
NAA1.86mg, basic element of cell division 2ip1.02mg, kanamycins 500mg, cephalosporin 500mg, agar 7.2g are settled to 1L,
PH=5.7；Condition of culture: cultivating 14 days under 2000Lux light, and illumination/dark is 16h/8h)
(2) pass through Selective agar medium, when explant grows small young plant, whole small young plant is cut, root restriction is erected
Straight cutting is into culture medium on MS culture medium, root induction.After the completion of under growth root, positive mirror is carried out to each independent transformation
It is fixed.(see Fig. 6)
The independent transformation strain 5 of high expression level and the independent transformants of low expression level are chosen by semiquantitive PCR
It is No. 3 progress salt resistance experiments.(see Fig. 7)
(3) tobacco is subjected to salt treatment
The uniform No. 5 transgenosis height of growing way for growing 7 days are expressed into tobacco, No. 3 transgenosis low expression tobaccos, wild type cigarettes
Grass transplanting is into native basin, and after native basin seedling is grown 5 days, every kind of plant retains 21 plants of consistent seedling of growing way, is randomly divided into three groups,
Every group of each 7 plants of different types of plant carries out pouring processing with salinity treatment fluid (150mM NaCl aqueous solution aqueous solution).3 days
Pour primary, each irrigation amount is 0.5 times of soil quality, and to keep the constant for the treatment of fluid concentration in basin, coprocessing is seen after 30 days
It examines plant and takes a picture (see Fig. 8).
SEQUENCE LISTING
<110>University Of Tianjin
<120>sedum lineare resistant gene of salt SLOACPS and its application
<130>
<160> 11
<170> PatentIn version 3.3
<210> 1
<211> 579
<212> DNA
<213> Sedum lineare Thunb
<400> 1
atgtctaggt cgaaactacc attcactgtc tcaccttttg cccacgaacg ctcctctatg 60
gccccttcct gttctccacg cacgtatgca gtggaaacat gtacacagac catcaaagca 120
catttcttag caaactccag agaatgtaat ggtcctgatg tgttgatagc caaagcaaca 180
tcatatcttt catcgaagtt ggtggttcca gctacattaa tcaccacctc aatgtcattc 240
cacatttcct tctttaagtt tgcatcttca attcccaaat ccttgaaaga tacatcccca 300
gggacaggaa ctaggactct gttgttgaca aggttactca gatctgagcc ccgctgttct 360
ttcagtaatc caaatagttc tttcccgagt atctcacttt gtaggcgttt agtagcggca 420
atagaatcct gtgctcttac tagaagaagc ctgcggatct ttggcactct cagtaacttc 480
tccacaagaa ctatatatat tacagcactt tctgtcagat tatctaagaa tatgatatgt 540
gaaactgcgt atgtaaggtg tgacaggaaa acagtataa 579
<210> 2
<211> 192
<212> PRT
<213> Sedum lineare Thunb
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Met Ser Arg Ser Lys Leu Pro Phe Thr Val Ser Pro Phe Ala His Glu
1 5 10 15
Arg Ser Ser Met Ala Pro Ser Cys Ser Pro Arg Thr Tyr Ala Val Glu
20 25 30
Thr Cys Thr Gln Thr Ile Lys Ala His Phe Leu Ala Asn Ser Arg Glu
35 40 45
Cys Asn Gly Pro Asp Val Leu Ile Ala Lys Ala Thr Ser Tyr Leu Ser
50 55 60
Ser Lys Leu Val Val Pro Ala Thr Leu Ile Thr Thr Ser Met Ser Phe
65 70 75 80
His Ile Ser Phe Phe Lys Phe Ala Ser Ser Ile Pro Lys Ser Leu Lys
85 90 95
Asp Thr Ser Pro Gly Thr Gly Thr Arg Thr Leu Leu Leu Thr Arg Leu
100 105 110
Leu Arg Ser Glu Pro Arg Cys Ser Phe Ser Asn Pro Asn Ser Ser Phe
115 120 125
Pro Ser Ile Ser Leu Cys Arg Arg Leu Val Ala Ala Ile Glu Ser Cys
130 135 140
Ala Leu Thr Arg Arg Ser Leu Arg Ile Phe Gly Thr Leu Ser Asn Phe
145 150 155 160
Ser Thr Arg Thr Ile Tyr Ile Thr Ala Leu Ser Val Arg Leu Ser Lys
165 170 175
Asn Met Ile Cys Glu Thr Ala Tyr Val Arg Cys Asp Arg Lys Thr Val
180 185 190
<210> 3
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acgggggact ctagaggatc c 21
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cgatcgggga aattcgagct c 21
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gaacttacta gccgactg 18
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cctcaagcct tatacgcaa 19
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<210> 8
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ttatactgtt ttcctgtcac acc 23
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acgggggact ctagaggatc catgtctagg tcgaaactac cattc 45
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cgatcgggga aattcgagct cttatactgt tttcctgtca cacc 44
<210> 11
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<400> 11
atgtctaggt cgaaactacc attcactgtc tcaccttttg cccacgaacg ctcctctatg 60
gccccttcct gttctccacg cacgtatgca gtggaaacat gtacacagac catcaaagca 120
catttcttag caaactccag agaatgtaat ggtcctgatg tgttgatagc caaagcaaca 180
tcatatcttt catcgaagtt ggtggttcca gctacattaa tcaccacctc aatgtcattc 240
cacatttcct tctttaagtt tgcatcttca attcccaaat ccttgaaaga tacatcccca 300
gggacaggaa ctaggactct gttgttgaca aggttactca gatctgagcc ccgctgttct 360
ttcagtaatc caaatagttc tttcccgagt atctcacttt gtaggcgttt agtagcggca 420
atagaatcct gtgctcttac tagaagaagc ctgcggatct ttggcactct cagtaacttc 480
tccacaagaa ctatatatat tacagcactt tctgtcagat tatctaagaa tatgatatgt 540
gaaactgcgt atgtaaggtg tgacaggaaa acagtataa 579
Claims (6)
1. sedum lineare resistant gene of salt SLOACPS, it is characterized in that nucleotide sequence shown in SEQ ID NO.1 in sequence table.
2. the cloning vector pJET1.2_SLOACPS of sedum lineare resistant gene of salt SLOACPS containing claim 1 a kind of.
3. the host cell containing cloning vector pJET1.2_SLOACPS.
4. the expression vector pBI121_SLOACPS of sedum lineare resistant gene of salt SLOACPS containing claim 1 a kind of.
5. the host cell containing expression vector pBI121_SLOACPS.
6. the purposes that sedum lineare resistant gene of salt SLOACPS enhances arabidopsis or Tobacco Salt performance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710712875.8A CN109402138A (en) | 2017-08-18 | 2017-08-18 | Sedum lineare resistant gene of salt SLOACPS and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710712875.8A CN109402138A (en) | 2017-08-18 | 2017-08-18 | Sedum lineare resistant gene of salt SLOACPS and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109402138A true CN109402138A (en) | 2019-03-01 |
Family
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| CN201710712875.8A Pending CN109402138A (en) | 2017-08-18 | 2017-08-18 | Sedum lineare resistant gene of salt SLOACPS and its application |
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| CN101747419A (en) * | 2008-12-08 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Protein related to salt tolerance, coding gene thereof and application thereof |
| CN103387994A (en) * | 2013-07-12 | 2013-11-13 | 南京农业大学 | Malus hupehensis MhWRKY40a gene and applications thereof |
| CN104611346A (en) * | 2015-02-16 | 2015-05-13 | 天津大学 | Salt-tolerant group and recombinant vector comprising same |
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| CN101747419A (en) * | 2008-12-08 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Protein related to salt tolerance, coding gene thereof and application thereof |
| CN103387994A (en) * | 2013-07-12 | 2013-11-13 | 南京农业大学 | Malus hupehensis MhWRKY40a gene and applications thereof |
| CN104611346A (en) * | 2015-02-16 | 2015-05-13 | 天津大学 | Salt-tolerant group and recombinant vector comprising same |
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