CN109401941A - A kind of genetic chip - Google Patents
A kind of genetic chip Download PDFInfo
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- CN109401941A CN109401941A CN201811357150.2A CN201811357150A CN109401941A CN 109401941 A CN109401941 A CN 109401941A CN 201811357150 A CN201811357150 A CN 201811357150A CN 109401941 A CN109401941 A CN 109401941A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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Abstract
The invention discloses a kind of genetic chips, including the first chip region, the first chip, the second chip and the second chip region successively to connect on thickness direction；Wherein, first chip is located on the first chip region, second chip is located on the second chip region, and first chip region one end and the rotation connection of the second chip region, the other end are equipped with first handle, and second chip region one end and the rotation connection of the first chip region, the other end are equipped with second handle.A kind of genetic chip of the present invention, does not need special Storage Box, just can effectively prevent pollution；And this genetic chip does not need to hybridize after being shifted amplified production when being hybridized, and can directly be hybridized in amplification system, and pollution of the sample in transfer process is avoided；It is simple for structure, it is easy to use, convenient for storage.
Description
Technical field
The present invention relates to a kind of genetic chips, belong to genetic chip field.
Background technique
With the implementation of human genome (sequencing) plan and the fast development of molecular biology related discipline, increasingly
More animals and plants, microbial genome sequence are measured, and gene sequence data increases rapidly at an unprecedented rate.
Genechip detection is because it is high-throughput, the feature of detection rapidly and efficiently is widely used to the Clinics and Practices of disease, drug sieve
The fields such as choosing, judicial expertise, environment measuring, the technology are that the nucleic acid probe molecules of a large amount of known arrays are fixed on support
Hybridized afterwards with the sample molecule of label, the hybridization signal intensities by detecting each probe molecule obtain sample molecule
Quantity and sequence information.
Existing conventional gene chip detecting technique needs first to carry out inhereditary material in nucleic acid amplification reaction container
Amplified production is sucked out after reaching certain enriching quantity for PCR amplification, is added on genetic chip and carries out chip probe hybridization point
Analysis.It polluting in order to prevent, existing genetic chip needs storage in special holder, the increase of cost is not only resulted in, and
And need to occupy a large amount of space；Further, when detection, the multiple transfer for expanding sample is easy to bring pollution, influences detection knot
Fruit.
Summary of the invention
The problems such as in order to solve cost caused by genetic chip in the prior art needs to store in special holder, this
Invention provides a kind of genetic chip.
In order to solve the above technical problems, the technical solution adopted in the present invention is as follows:
A kind of genetic chip, including successively connect on thickness direction the first chip region, the first chip, the second chip and
Two chip regions；Wherein, the first chip is located on the first chip region, and the second chip is located on the second chip region, the first chip region one
End with the second chip region rotation connection, the other end be equipped with first handle, second chip region one end and the first chip region rotation connection,
The other end is equipped with second handle.
Described herein is that the first chip region of gene piece and the second chip region are in folded state and place are not used
In the structure for the state of sealing up for safekeeping, at this point, the first chip and the second chip be between the first chip region and the second chip region, it can be to
One chip and the second chip play a very good protection, and without special holder, reduce costs, and save space, and
It is easy to use.
Between above-mentioned first chip region and the second chip region for 360 ° rotation connection, when genetic chip when not in use, pass through
Handle rotates chip region, closes the first chip and the second chip lid between the first chip region and the second chip region, is saved；
When needing using genetic chip, chip region is rotated by handle, is made the first chip and the second chip (namely thickness side outwardly
To being successively the first chip, the first chip region, the second chip region and the second chip), after PCR, handle is held, by this base
Hybridization reaction can directly be started after being inserted into PCR reaction system because of chip, when after reaction, upward handle takes chip
Out, after the first chip region and the second chip region being deployed in same level, it is put into detector read output signal.
In order to improve the stability that genetic chip is used and saved, the first chip region is being equipped with one end of first handle just
Reverse side is equipped with buckle, and the front and back sides that the second chip region is equipped with one end of second handle are equipped with button hole, buckle and button hole
Position is opposite and is mutually matched.
When the first chip region and the second chip region both turn to fitting, no matter the first chip and the second chip are inwardly
Still outwardly, buckle can be all caught in button hole and guarantee stability.The application is by the working face of the first chip region and the second chip region
Between be defined as in.
As another scheme of the application, the front and back sides that the first chip region is equipped with one end of first handle are equipped with evil spirit
Art pastes hair side, and the front and back sides that the second chip region is equipped with one end of second handle are equipped with velcro hook surface, velcro hair side with
Velcro hook surface shape is identical, equal in magnitude, position is opposite and is mutually matched.When the first chip region and the second chip region rotate
To when the two fitting, no matter the first chip and the second chip are that inwardly or outwardly, all velcro hair side can be attached in hook surface
Guarantee stability.
In order to facilitate use, and preventing from polluting when sealing up for safekeeping, first handle and second handle are strip structure, and the
One handle is identical with second handle shape, equal in magnitude, position is opposite.Namely when the first chip region and the second chip region are in folding
When overlapping state, first handle and second handle can be completely coincident.
In order to prevent polluting when not saving, the first chip region identical, equal in magnitude, position phase with the second chip region shape
It is right.
The pollution caused by the transfer of amplified matter in order to prevent, the first chip and the second chip structure are identical, and first
It is equipped with probe array on chip and the second chip, includes the hybridization column of an at least row and a column in probe array, by probe battle array
Column are divided into different detection zones, and hybridization column is matched by complementary gene and formed, for different pattern detection areas carry out every
It is disconnected；Each detection zone includes negative control probe, positive control probe and detection probe.Mutual interference is avoided, and logical
The division for crossing hybridization column, can be used to detect multiple and different genes.
When genetic chip when not in use, chip region is rotated by handle, closes the first chip and the second chip lid first
Between chip region and the second chip region, button hole is buckled into, is saved；When needing using genetic chip, turned by handle
Dynamic chip region, makes that (namely thickness direction is successively the first chip, the first chip region, the outwardly by the first chip and the second chip
Two chip regions and the second chip), button hole is buckled into, Gai He holds handle after PCR, this genetic chip is inserted into
Hybridization reaction can directly be started after PCR reaction system, when after reaction, upward handle takes out chip, by the first core
After section and the second chip region are deployed in same level, it is put into detector read output signal.
It include the hybridization column of a row and two column in order to reduce volume and be able to satisfy primary demand, in probe array, by probe
Array is divided into 6 different detection zones.
It is suitable for gene-detecting apparatus for the ease of this genetic chip, first handle is detachably connected on the first chip region,
Second handle is detachably connected on the second chip region.Handle can be dismounted as needed in this way.
As another preferred embodiment, the first chip is detachably connected on the first chip region, and the second chip detachably connects
It connects on the second chip region.Chip can be dismounted as needed in this way.
In order to facilitate rotating, reducing cost, the first chip region and the second chip region are rotatably connected by webbing.
In order to preferably prevent polluting, webbing covers the width direction of the first chip region and the second chip region.
It is polluted to not only can guarantee the patency of rotation but also can prevent, knitting between the first chip region and the second chip region
The length of object band is 1-2mm.
The unmentioned technology of the present invention is referring to the prior art.
A kind of genetic chip of the present invention, does not need special Storage Box, just can effectively prevent pollution；And this genetic chip exists
It when being hybridized, does not need to hybridize after being shifted amplified production, can directly be hybridized in amplification system, be avoided
Pollution of the sample in transfer process；It is simple for structure, it is easy to use, convenient for storage.
Detailed description of the invention
Fig. 1 is genetic chip structural schematic diagram of the present invention；
Fig. 2 is genetic chip unfolded state schematic diagram of the present invention；
Fig. 3 is the first chip (or second chip) structural schematic diagram；
In figure, 1 is the first chip region, and 2 be the second chip region, and 3 be the first chip, and 4 be the second chip, and 5 be buckle, and 6 are
Button hole, 7 be first handle, and 8 be second handle, and 9 be webbing, and 10 be hybridization column, and 11 be negative control probe, and 12 be positive right
According to probe, 13 be detection probe.
Specific embodiment
For a better understanding of the present invention, below with reference to the embodiment content that the present invention is furture elucidated, but it is of the invention
Content is not limited solely to the following examples.
Embodiment 1
A kind of genetic chip, including successively connect on thickness direction the first chip region, the first chip, the second chip and
Two chip regions；Wherein, the first chip is located on the first chip region, and the second chip is located on the second chip region, the first chip region one
End with the second chip region rotation connection, the other end be equipped with first handle, second chip region one end and the first chip region rotation connection,
The other end is equipped with second handle.
It is above-mentioned described to be the first chip region of gene piece and the second chip region be in folded state and unused is in
Seal the structure of state up for safekeeping, at this point, the first chip and the second chip be between the first chip region and the second chip region, it can be to first
Chip and the second chip play a very good protection, and without special holder, reduce costs, and save space, and make
With conveniently.
Between above-mentioned first chip region and the second chip region for 360 ° rotation connection, when genetic chip when not in use, pass through
Handle rotates chip region, closes the first chip and the second chip lid between the first chip region and the second chip region, is saved；
When needing using genetic chip, chip region is rotated by handle, is made the first chip and the second chip (namely thickness side outwardly
To being successively the first chip, the first chip region, the second chip region and the second chip), after PCR, handle is held, by this base
Hybridization reaction can directly be started after being inserted into PCR reaction system because of chip, when after reaction, upward handle takes chip
Out, after the first chip region and the second chip region being deployed in same level, it is put into detector read output signal.
Embodiment 2
It is substantially the same manner as Example 1, except that: in order to improve the stability that genetic chip is used and saved, first
The front and back sides that chip region is equipped with one end of first handle are equipped with buckle, and the second chip region is equipped with one end of second handle
Front and back sides are equipped with button hole, buckle opposite with the position of button hole and are mutually matched.When the first chip region and the second chip region rotate
To when the two fitting, no matter the first chip and the second chip are inwardly or outwardly, buckle can be all caught in button hole and guarantee to stablize
Property.
Embodiment 3
It is substantially the same manner as Example 1, except that: the front and back sides that the first chip region is equipped with one end of first handle are equal
Equipped with velcro hair side, the front and back sides that the second chip region is equipped with one end of second handle are equipped with velcro hook surface, velcro
Hair side is identical as velcro hook surface shape, equal in magnitude, position is opposite and is mutually matched.When the first chip region and the second chip
When area both turns to fitting, no matter the first chip and the second chip are inwardly or outwardly, can be all attached to velcro hair side
Guarantee stability in hook surface.
Embodiment 3
It is substantially the same manner as Example 2, except that: first handle and second handle are strip structure, and first
Handle is identical with second handle shape, equal in magnitude, position is opposite.Namely when the first chip region and the second chip region are in and fold
When state, first handle and second handle can be completely coincident.
Embodiment 4
It is substantially the same manner as Example 3, except that: the first chip region is identical, equal in magnitude with the second chip region shape,
Position is opposite.
Embodiment 5
It is substantially the same manner as Example 4, except that: the pollution caused by the transfer of amplified matter in order to prevent, the
One chip and the second chip structure are identical, and probe array is equipped on the first chip and the second chip, include one in probe array
Probe array is divided into 6 different detection zones by the hybridization column of row and two column, and hybridization column is matched by complementary gene and formed,
For separating to different pattern detection areas；Each detection zone includes negative control probe, positive control probe and inspection
Probing needle.Mutual interference is avoided, and by the division on hybridization column, can be used to detect multiple and different genes.
When genetic chip when not in use, chip region is rotated by handle, closes the first chip and the second chip lid first
Between chip region and the second chip region, button hole is buckled into, is saved；When needing using genetic chip, turned by handle
Dynamic chip region, makes that (namely thickness direction is successively the first chip, the first chip region, the outwardly by the first chip and the second chip
Two chip regions and the second chip), button hole is buckled into, Gai He holds handle after PCR, this genetic chip is inserted into
Hybridization reaction can directly be started after PCR reaction system, when after reaction, upward handle takes out chip, by the first core
After section and the second chip region are deployed in same level, it is put into detector read output signal.
Embodiment 6
It is substantially the same manner as Example 5, except that: for the ease of this genetic chip be suitable for gene-detecting apparatus, first
Handle is detachably connected on the first chip region, and second handle is detachably connected on the second chip region.It in this way can be as needed
Handle is dismounted.
Embodiment 7
It is substantially the same manner as Example 5, except that: the first chip is detachably connected on the first chip region, the second core
Piece is detachably connected on the second chip region.Chip can be dismounted as needed in this way.
Embodiment 8
It is substantially the same manner as Example 6, except that: the first chip region and the second chip region are rotatablely connected by webbing
Together, the width direction of webbing covering the first chip region and the second chip region, between the first chip region and the second chip region
Webbing length be 1.5mm.
The genetic chip of above-mentioned each example, does not need special Storage Box, just can effectively prevent pollution；And this genetic chip exists
It when being hybridized, does not need to hybridize after being shifted amplified production, can directly be hybridized in amplification system, be avoided
Pollution of the sample in transfer process；It is simple for structure, it is easy to use, convenient for storage.
Application examples 1
The present embodiment genetic chip is complete using 1.0 ST of Affymetrix Gene that affymetrix company in the U.S. provides
Genomic oligonucleotide chip contains 17000 bases containing 33 298 mankind's knowns from Unigene Genbank
Because of probe.By the built-in chip type on chip region of the invention, respectively there are a chip in the first chip region and the second chip region, because
This, can once go out two groups of data.The structure of genetic chip is as described in Example 8.
Step 1: taking the mucous membrane of nasopharynx tissue of Nasopharyngeal Carcinoma Patients and normal individual, carries out the extraction of total serum IgE respectively
(TRIzol)。
It takes 200mg tissue that 2ml TRIzol is added, tissue is homogenized, the sample to homogenize places 5 points at 15~30 DEG C
Then the chloroform of 0.2 times of volume is added in clock, mix, and places at 15~30 DEG C 2~3 minutes, and 4 DEG C of 7,000rpm are centrifuged 15 minutes,
It taking upper strata aqueous phase that the isopropanol of 0.8 times of volume is added to a new centrifuge tube, mixes, 4 DEG C of 7,000rpm are centrifuged 15 minutes,
Abandon supernatant.70% alcohol is added, reverse centrifuge tube precipitates contained salinity for several times to wash away, and then 4 DEG C of 7,000rpm are centrifuged 10 points
Clock abandons supernatant.It repeats the above steps 1 time, the water dissolution of suitable no RNase is added into centrifuge tube.It is scored with spectrophotometric
RNA concentration is analysed, is approximately equal to the RNA of 40ug/ml in OD260.Its ODA260/A280 is needed to should be 1.9~2.1.With agarose electricity
Whether there is or not degradations by swimming detection RNA, then save backup for -80 DEG C.
Step 2: the label of fragmentation sample
Single stranded DNA fragmentation is used into following fragmentation system: 5.5 μ g Single- for cDNA by RNA reverse transcription
Stranded DNA (single stranded DNA), 4.8 μ l10XcDNA Fragmentation Buffer (10XcDNA fragmentation buffer),
1.0 1.0 μ lAPE of μ L UDG, 10U/ μ L, 1,1,000U/ μ L RNase-free Water to 48 μ L is mixed；37 DEG C, 60 points
Clock；93 DEG C, 2 minutes；4 DEG C, at least 2 minutes；Liquid on lower wall is got rid of, is mixed；45 μ L samples are shifted into a new centrifuge tube.
Remaining sample is for detecting, and the range of segment size is in 40-70bp.It marks at once, -20 DEG C of preservation samples.By following reagent body
System: 45 μ L Fragmented Single-Stranded DNA, 12 μ L5x TdT Buffer, 2 μ L TdT, 1 μ LDNA
60 μ L of Labeling Reagent, 5mM total volume is added in the sample of fragmentation, is mixed；37 DEG C, 60 minutes；70 DEG C, 10 points
Clock；4 DEG C, at least 2 minutes, are marked, stand-by after detection reference numerals.
Step 3: post-hybridization washing
Genetic chip of the invention is put into the segment label sample of step 2 and is hybridized, while following system is added
Hybridization solution: 3.7 μ L Control Oligonucleotide B2 (control oligonucleotide B2), 11 μ L 20X
Eukaryotic Hy bridization Controls (bioB, bioC, bioD, ere) (eucaryote chemical reference), 2.2 μ
L Herring Sperm DNA (10medmL) (herring sperm dna), 2.2 μ L Acetylated BSA (50mg/mL) (acetyl
Change BSA), 110 μ L 2X Hybridization Buffer (2X hybridization buffer), 15.4 μ L 7%DMSO, RNase free
H20 until 220 μ L of total system.5 DEG C, 60r/min, hybridize 16 hours.It is washed after hybridization.
Step 4: scanning and analysis
It is scanned using Gene array Scanner 3000, reads the expression value in each site of every chip, background correction
Signal, the quiding gene Data Analysis Software after software is handled automatically analyze the expression parameter of each gene loci and provide ginseng
Examine value, output data.
The total serum IgE sample for taking experimental procedure 1 to obtain, the above-mentioned detection of repetition is primary, obtains second group of data.
First time experimental result:
| Difference expression gene number | Raise 2 times or more | Lower 2 times or more | Summation |
| Normal tissue and cancerous tissue-chip 1 | 154 | 256 | 410 |
| Normal tissue and cancerous tissue-chip 2 | 154 | 255 | 409 |
Second of experimental result:
| Difference expression gene number | Raise 2 times or more | Lower 2 times or more | Summation |
| Normal tissue and cancerous tissue-chip 1 | 152 | 255 | 407 |
| Normal tissue and cancerous tissue-chip 2 | 152 | 257 | 409 |
By data it can be found that detection data through the invention more balances, the differential expression base of two chips between group
Because the variance of sum is respectively 0.25,1.00, the variance that difference expression gene sum is tested between group is 1.18, and fluctuation is not
Greatly.
Application examples 2:
The genetic chip of the present embodiment is identical as application examples 1, is provided using affymetrix company, the U.S.
1.0 ST Oligo microarray of Affymetrix Gene, primary experiment take 2 chip operation repetitives, the present embodiment
Chip operation take conventional operating method, do not place it in chip region of the invention.Step 1 and the same embodiment of step 2
One agents useful for same consumptive material and operating process, then prepare hybridization solution in the centrifuge tube of 1.5mL, step 2 are taken to obtain
Fragmented and Labeled DNA Target (DNA target mark of fragmentation and label) 60 μ L, 3.7 μ L Control
Oligonucleotide B2 (control oligonucleotide B2), 11 μ L 20X Eukaryotic Hy bridization
Controls (bioB, bioC, bioD, ere) (20X eucaryote chemical reference), 2.2 μ L Herring Sperm DNA
(10medmL) (herring sperm dna), 2.2 μ L Acetylated BSA (50mg/mL) (acetylation BSA), 110 μ L 2X
Hybridization Buffer (2X hybridization buffer), 15.4 μ L 7%DMSO supplement RNase free H20 until totality
It is 220 μ L.It is centrifuged after system is mixed, heats 99 DEG C of hybridization solution, 5min, 45 DEG C, 5min, high speed centrifugation 1min.Coring piece
Exon 1.0ST carries out equilibrium at room temperature, and chip is sticked fence using fence paste tool, draws 200uL hybridization solution to chip
On, 45 DEG C, 60r/min carries out hybridization 16 hours.Post-hybridization washing carries out data point using the identical analysis method of embodiment one
Analysis processing.
The total serum IgE sample for equally taking experimental procedure 1 to obtain, the above-mentioned detection of repetition is primary, obtains second group of data.
First time experimental result:
| Difference expression gene number | Raise 2 times or more | Lower 2 times or more | Summation |
| Normal tissue and cancerous tissue-chip 1 | 158 | 261 | 419 |
| Normal tissue and cancerous tissue-chip 2 | 163 | 253 | 416 |
Second of experimental result:
| Difference expression gene number | Raise 2 times or more | Lower 2 times or more | Summation |
| Normal tissue and cancerous tissue-chip 1 | 163 | 267 | 430 |
| Normal tissue and cancerous tissue-chip 2 | 152 | 254 | 406 |
By data it can be found that the variance of the difference expression gene sum of two chips is respectively 2.25,144 between group, group
Between test difference expression gene sum variance be 73.18, the application example compared with the data of application examples 1, either group in go back
It is to test between group, fluctuation is larger.The possible reason is chip is more by extraneous interference.
Application examples 3:
Chip used in the application example is the genetic chip independently synthesized, uses Genomic Solution company, the U.S.
Full-automatic point sample instrument, the substrate of chip uses the aldehyde radical substrate of Boao Biological Co., Ltd.
The present embodiment has chosen three kinds of viruses in GeneBank database, devises for three kinds of viral conservative regions
Genetic chip, one is traditional genetic chips individually saved, and one is genetic chips designed by the embodiment of the present invention 8
(the application example has only used the one side of genetic chip of the invention, i.e., there was only a chip in structure), passes through the inspection to virus
Survey the testing result for comparing two kinds of chips.
Tri- kinds of viruses of CCHFV, RVFV, LAV are had chosen, to from different regions, virulence viral gene of different strengths and weaknesses
Analyzed, pick the conservative region in three kinds of viral full-length genomes, by the region carry out it is artificial synthesized after, be inserted into pMD-
In 18T simple plasmid, recombination material pMD-CCHFV, pMD-RVFV and pMD-LVA are obtained, the synthesis of gene is by the raw work in Shanghai
It completes.In three kinds of viral conservative regions of synthesis, each design pair of primers, holds mark fluorescent element in primer 5 ' respectively.
The gene order of primer:
CCHFV-F:ATGAGATGAATAAGTGGTTTGAAGAG
CCHFV-R:GAGAGCAGCCTGTTGGTAATC
RVFV-F:TTAGAGGTTAAGGCTGCCC
RVFV-R:ACCTCCGAGGTAGAACAAAG
LAV-F:TTGACCTCTTTGTGTTCAGC
LAV-R:TCCACAGGAACAAATGCCC
Probe is designed in the amplification region of primer, is directed to respectively with Array Designer4.0 probe design software
The amplification region of CCHFV, RVFV, LAV design 2 probes.NCBI is logged in, designed probe is compared, is excluded
It is higher than 50% probe with other viral genome homologys.Ensure the homology between probe simultaneously also below 50%, for monitoring
Hybrid process designs a positive control probe and its corresponding target and negative probes.By the way that point sample program is arranged on point sample instrument,
Each probe designs 3 sites, completes the preparation of genetic chip.
Step 1: the amplification of sample and label
It chooses containing being that test sample is marked and expands there are three types of the cDNA of virus, three pairs of specific primers is mixed,
5 ' ends of downstream primer are modified by fluorescein, according to the multiplex PCR system that steps are as follows, carry out the amplification of sample.95 DEG C of pre- changes
Shape 5min；95 DEG C of 30s, 60 DEG C of 30s, 72 DEG C of 40s are recycled for 36 totally；72℃10min.
Step 2: hybridization reaction
Preparing hybrid buffer:
It is mixed in the ratio of PCR product and hybridization buffer 1:1, and appropriate positive targets is added, after careful mixing
Start hybridization reaction.Hybridization herein is divided into two kinds of comparative experimentss, and one is PCR product is sucked out to mix with hybridization buffer
Afterwards, solution suction after mixing is added on chip and carries out hybridization reaction；Another kind is that chip of the invention is put into PCR reaction
In system, hybridization reaction is directly carried out after hybridization buffer is added.After hybridization, successively chip is put in wash in washing lotion and be done
Centrifuge dripping after net, it is to be measured.
Step 3: scanning and analysis
The chip hybridized is placed on and is scanned under fluorescence microscope and obtains image.Using Photoshop software by image
Processing, and save as tiff format.Treated image is imported to the analysis software of GenepixPr06.0 genetic chip, analysis
The signal-to-noise ratio and absolute fluorescence value of acquisition.
+ indicate effective fluorescence signal
Effective fluorescence signal is not detected in expression
Pass through the analysis to testing result, it is found that using three kinds of viral cDNA as test sample, the fluorescence that detects
Signal is completely the same, without otherness.
The sample for having CCHFV, RVFV, LAV viral infection symptoms is chosen, carries out tradition according to the experimental procedure of the present embodiment
Chip detection and chip of the present invention detect two ways measurement result.
+ indicate effective fluorescence signal
Effective fluorescence signal is not detected in expression
Pass through the analysis to testing result, it is found that infect sample as test object, the fluorescence signal detected has
Difference, RVFV traditional die testing result is the positive, and chip test result of the invention is feminine gender, by infection sample
Band is verified after PCR amplification, and the amplified band of discovery sample not RVFV is further verified by serology, finds not feel
Contaminate RVFV virus.Therefore, traditional die detection is particularly likely that sample pollution in the analysis process causes there are false positive
's.
Claims (10)
1. a kind of genetic chip, it is characterised in that: including successively connect on thickness direction the first chip region, the first chip,
Two chips and the second chip region；Wherein, the first chip is located on the first chip region, and the second chip is located on the second chip region, the
One chip region one end and the rotation connection of the second chip region, the other end are equipped with first handle, second chip region one end and the first chip
Area's rotation connection, the other end are equipped with second handle.
2. genetic chip as described in claim 1, it is characterised in that: the first chip region is being equipped with one end of first handle just
Reverse side is equipped with buckle, and the front and back sides that the second chip region is equipped with one end of second handle are equipped with button hole, buckle and button hole
Position is opposite and is mutually matched；Or first chip region be equipped with the front and back sides of one end of first handle and be equipped with velcro hair side,
The front and back sides that second chip region is equipped with one end of second handle are equipped with velcro hook surface, velcro hair side and velcro hook surface
Shape is identical, equal in magnitude, position is opposite and is mutually matched.
3. genetic chip as claimed in claim 1 or 2, it is characterised in that: first handle and second handle are strip knot
Structure, and first handle is identical with second handle shape, equal in magnitude, position is opposite.
4. genetic chip as claimed in claim 1 or 2, it is characterised in that: the first chip region is identical with the second chip region shape,
It is equal in magnitude, position is opposite.
5. genetic chip as claimed in claim 1 or 2, it is characterised in that: the first chip and the second chip structure are identical, and first
It is equipped with probe array on chip and the second chip, includes the hybridization column of an at least row and a column in probe array, by probe battle array
Column are divided into different detection zones, and hybridization column is matched by complementary gene and formed；Each detection zone include negative control probe,
Positive control probe and detection probe.
6. genetic chip as claimed in claim 5, it is characterised in that: it include the hybridization column of a row and two column in probe array,
Probe array is divided into 6 different detection zones.
7. genetic chip as claimed in claim 1 or 2, it is characterised in that: first handle is detachably connected on the first chip region
On, second handle is detachably connected on the second chip region.
8. genetic chip as claimed in claim 1 or 2, it is characterised in that: the first chip is detachably connected on the first chip region
On, the second chip is detachably connected on the second chip region.
9. genetic chip as claimed in claim 1 or 2, it is characterised in that: the first chip region and the second chip region pass through fabric
Band is rotatably connected.
10. genetic chip as claimed in claim 9, it is characterised in that: webbing covers the first chip region and the second chip region
Width direction；The length of webbing between first chip region and the second chip region is 1-2mm.
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| CN201811357150.2A CN109401941B (en) | 2018-11-15 | 2018-11-15 | Gene chip |
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| US20040071605A1 (en) * | 2002-10-10 | 2004-04-15 | Coonan Everett W. | Slide-based high-throughput microplate device |
| CN105296328A (en) * | 2015-10-28 | 2016-02-03 | 北京中科紫鑫科技有限责任公司 | DNA sequencer reaction chip device |
| CN206248665U (en) * | 2016-11-25 | 2017-06-13 | 吉林农业科技学院 | A kind of parasite detection kit |
| CN209442999U (en) * | 2018-11-15 | 2019-09-27 | 南京吉量生物科技有限公司 | A kind of genetic chip |
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2018
- 2018-11-15 CN CN201811357150.2A patent/CN109401941B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040071605A1 (en) * | 2002-10-10 | 2004-04-15 | Coonan Everett W. | Slide-based high-throughput microplate device |
| CN105296328A (en) * | 2015-10-28 | 2016-02-03 | 北京中科紫鑫科技有限责任公司 | DNA sequencer reaction chip device |
| CN206248665U (en) * | 2016-11-25 | 2017-06-13 | 吉林农业科技学院 | A kind of parasite detection kit |
| CN209442999U (en) * | 2018-11-15 | 2019-09-27 | 南京吉量生物科技有限公司 | A kind of genetic chip |
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| CN109401941B (en) | 2024-05-28 |
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