CN109396165A - A method of with the restoring cadmium polluted agricultural land soil of beggar-ticks - Google Patents
A method of with the restoring cadmium polluted agricultural land soil of beggar-ticks Download PDFInfo
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- CN109396165A CN109396165A CN201811425833.7A CN201811425833A CN109396165A CN 109396165 A CN109396165 A CN 109396165A CN 201811425833 A CN201811425833 A CN 201811425833A CN 109396165 A CN109396165 A CN 109396165A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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Abstract
The present invention is a kind of method for repairing farmland cadmium pollution soil with beggar-ticks, includes the following steps: one, proves absorption of the Ageratum conyzoides to cadmium；Two, operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering is proved.Advantages of the present invention: the present invention is shown by test data: beggar-ticks has been above 1 to the concentration coefficient BF and transhipment coefficient T F of Cd, and it is 12.9%~18.6% that beggar-ticks, which repairs Cd contaminated soil to plant the removal rate of three batches of Ageratum conyzoides per acre, in field trials.Thus illustrate that beggar-ticks repairs with good application prospect agricultural land soil cadmium pollution；The features such as present invention, which has, to repair thoroughly, adaptable, without secondary pollution.
Description
Technical field
The present invention relates to a kind of methods with the restoring cadmium polluted agricultural land soil of beggar-ticks, belong to soil pollution by heavy metal
Recovery technique field.
Background technique
In recent years, since Industrial " three Waste " and fertilizer containing Cd are largely applied, a large amount of heavy metal Cd causes after entering soil
Cd pollution in soils is on the rise, and Cd is as heavy metal poisoning and the strongest one kind of migration, through food chain in human body
It accumulates and causes damages.Therefore, particularly significant is become to the reparation of Cd polluted farmland soil.With traditional heavy metal pollution reparation
It is heavy metal-polluted that the advantages that method is compared, and the phytoremediation technology of heavy metal pollution is without secondary pollution with its, inexpensive becomes various countries
The hot spot in improvement field is contaminated, and the selection of rehabilitation plant is the most important content of phytoremediation.Currently, having reported in the literature
Super enriching plant has Sedum alfredii Hance, Viola baoshanensis, Indian mustard etc., but phytoremediation is still not mature enough, and most plants still stop
Stay in the stage in laboratory.The remediation efficiency of heavy metals in farmland pollution is also with region, and weather, the factors such as soil are related, therefore
Field test results can provide preferable scientific basis to actual engineering reclamation activities.Cd pollution for different regions
It is more crucial to find suitable rehabilitation plant for the reparation in farmland.Sedum (Sedum alfredii Hance, eight treasures (choice ingredients of certain special dishes) scape having now been found that
It) the boisterous ability of plant resistant is weaker, do not have in this research in the eight treasures (choice ingredients of certain special dishes) red-spotted stonecrop overground part Cd content of field trial plantation
There is performance obvious that advantage, field management are complex.Indian mustard, Viola baoshanensis etc. have good Cd accumulation ability, but
Its biomass is smaller, therefore can not also promote in actual engineer application.Trade route, black nightshade, feather cockscomb etc. is all also in laboratory rank
Section, distance promote also a distance.Weeds especially farmland weed is generated under a kind of man and nature selection dual-pressure
The plant group that height is evolved, compared with crop, the degeneration-resistant border ability of weeds is stronger, by long-term natural evolution and manually
Selection, with extensive adaptability and indomitable vitality, while weeds also have it is stronger win honour for, strive water, strive fertile ability.
Beggar-ticks is distributed in the most area of China as a kind of weeds in field, annual herb, likes warm and moist weather, road
Side, house side and wilderness can be grown, and be civil common herbal medicine.With good application prospect to reparation agricultural land soil.
Summary of the invention
Proposed by the present invention is a kind of method with the restoring cadmium polluted agricultural land soil of beggar-ticks, is intended to repair agricultural land soil
Cadmium pollution.
The technical scheme is that a kind of method with the restoring cadmium polluted agricultural land soil of beggar-ticks, includes the following steps:
Step 1: proving absorption of the beggar-ticks to Cd stress；
Step 2: proving operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering.
Preferably, step 1 proves absorption of the beggar-ticks to Cd stress, specifically includes: beggar-ticks seedling replanting is entered by cadmium
In polluted farmland soil, by the way of outdoor cultivation.
Preferably, step 2 proves operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering, tool
Body includes: by Bidens bipinnata grass seed by line-spacing 20cm, using drilling method (1.5kg mu -1), the mode of field cultivation, deep ploughing
Soil uniformly applies compound fertilizer as base manure, and mixed to apply rear rotary tillage, periodically watering, keeping field capacity is 65~80%, at
60 days harvesting overground part beggar-ticks after ripe then plant second batch three batches, repeat the above process, until repairing polluted agricultural land soil
Earth.
Advantages of the present invention: beggar-ticks beggar-ticks in pot experiment processing group T1 and T2 to the concentration coefficient BF of cadmium and moves
It moves coefficient T F and has been above 1, beggar-ticks has preferable absorption and turn-over capacity to cadmium to the enriched character of Cd.It is tried in field
In testing, overground part Cd content average value is 10.94mgkg-1, maximum value 14.34mgkg-1, concentration coefficient average value is
4.16, maximum value 5.94.Planting one batch per acre and taking away heavy metal in soil is about 9.2~13.1g.Every kind of one batch of ghost per acre
Needle grass can take away 4.3%~6.2% Cd in contaminated area soil.Survey region is middle subtropical zone monsoon region, and heat is high,
Rainfall is big, and the sunshine-duration is long, meets the growth demand of beggar-ticks, therefore can plant 3 batches of beggar-ticks every year, is not adding activation
In the case where agent, the removal rate for planting 3 batches of beggar-ticks per acre is 12.9%~18.6%.
Specific embodiment
A method of with the restoring cadmium polluted agricultural land soil of beggar-ticks, include the following steps:
One, absorption of the beggar-ticks to Cd stress is proved；It specifically includes
The design of 1 experiment process:
The agricultural land soil (T1, T2 group) and cleaning soil (CK) of two kinds of practical cadmium pollution degree are taken, 4mm sieve is crossed, it is sufficiently mixed
After even, it is spare in flowerpot to weigh 3.5kg soil, each processing be respectively set four it is parallel.By Bidens bipinnata grass seed and sand 1:1
Sowing is in flowerpot after mixing, watering, after planting 15 thinning, leaves and takes three young plant similar in growing way in every basin.It is basic for examination soil
Property is as follows:
Table 1 is for trying soil labile organic matter.
| Refer to Mark | pH | Cd(mg· kg-1) | DTPA-Cd (mg·kg-1) | Organic matter (g kg-1) | Alkali-hydrolyzable nitrogen (mg kg-1) | Available phosphorus (mg kg-1) | Available potassium (mg·kg-1) |
| CK | 7. 20 | 0.45 | 0.25 | 21.2 | 98.5 | 22.45 | 141.36 |
| T1 | 6. 78 | 3.53 | 1.99 | 24.5 | 103.3 | 21.47 | 131.31 |
| T2 | 6. 54 | 13.37 | 10.50 | 22.7 | 102.2 | 21.32 | 133.42 |
2, experiments have shown that absorption of the beggar-ticks to cadmium:
The measurement of cadmium concentration in beggar-ticks organ, material gather in when, plant is divided into root, overground part two parts, spend from
Sub- water is put into culture dish after rinsing 3 times, after drying to constant weight at finishing 15 minutes, 60 DEG C in 80 DEG C of baking oven, uses electronics
Assay balance accurately weighs 0.2g (being accurate to 0.0001g) beggar-ticks sample in 50mL conical flask, adds 10mL nitration mixture (dense
HNO3: HCIO4=9:1), add the small funnel epiphragma in a labyrinth, impregnate after two nights in being cleared up on electric hot plate, temperature is set as when beginning
120 DEG C, after brown color cigarette is emerged in bottle, gradually rise temperature to 160 DEG C (actual temperature is preferably not more than 175 DEG C), often
Or so half an hour observation is primary, and adjusting conical flask position in time makes sample homogeneous heating, if there is crystallization to clear up incomplete object in bottle
Matter, then plus mixed acid boil to 2~4mL of residue and disappear boil liquid, remove and let cool until digestive juice is in colorless and transparent or yellowish,
It is settled in 25mL color-comparison tube with ultrapure water, uses Inductively coupled plasma optical emission spectrometer and graphite furnace-Atomic absorption
Spectrographic determination cadmium content, the dry weight meter of constituent content corresponding organ；Data result is shown in Table 2；
Enrichment condition (mgkg of the 2 potted plant experiment different disposal group beggar-ticks of table to cadmium-1)
Note: content=Mean ± SD, n=4.
Research plant repairs heavy-metal contaminated soil, not only to see that plant accumulates the patience and coefficient of heavy metal
Ability, it is also contemplated that the ability of its overground part transhipment heavy metal；Concentration coefficient BF reflects heavy metal in soil plant system
The complexity of middle migration；Transport coefficient TF usually can reflect the transport and distribution condition of heavy metal in vivo, in numerical value
Reflect the ability that plant transports heavy metal under ground portion to aerial part in feature；Concentration coefficient is bigger, shows plant
Accumulation ability is stronger；Transport coefficient is bigger, shows that plant is stronger to the absorption migration ability of heavy metal, i.e., to contaminated soil
Repair ability is stronger, and beggar-ticks is shown in Table 3 to concentration coefficient BF and transport coefficient TF the result statistics of cadmium:
Concentration coefficient BF and transport coefficient TF of 3 beggar-ticks of table to cadmium
| Processing group | Overground part concentration coefficient | Transport coefficient |
| CK | 1.66±0.75b | 1.31±0.67a |
| T1 | 4.70±0.86a | 1.59±0.40a |
| T2 | 3.51±0.63a | 0.73±0.29a |
Statistics indicate that: beggar-ticks has been above 1 in concentration coefficient BF and transport coefficient TF of the processing group T1 and T2 to cadmium, ghost
Needle grass has preferable absorption and turn-over capacity to cadmium to the enriched character of Cd.
Two, it proves operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering, specifically includes,
1, experimental design
1 mu or so of the farmland progress field experiment cell for selecting certain region to be polluted by Cd.In view of experimental field being tried in field
Importance during testing, selection is sunny, surrounding is spacious, convenient for management, and the ground of relatively flat is tested.Deep ploughing soil
Earth uniformly applies compound fertilizer as base manure, and mixed to apply rear rotary tillage, periodically watering, keeping field capacity is 80% or so.It is above-mentioned
After soil aging 1 week, beggar-ticks is sowed, using (1.5kg mus of drilling method-1), due to Bidens bipinnata grass seed very little, when sowing
It is broadcast again after being mixed using seed and sand 1:1, line-spacing 20cm, steps on print by line-spacing bracing wire, water furrow planting, Ying Jinliang when sowing
Guarantee sowing uniformly, the depth is consistent, avoids leakage kind, 0.5~1cm of earthing.About 15 days after plant final singling, appropriate thinning keeps every
About 6000 plants of beggar-ticks of mu.In growth course, base manure is suitably applied, adds 7~8kg of urea per acre, plant is kept normally to seek
The demand of supporting, and appropriate weeding acquire beggar-ticks overground part sample, and determination of yield is carried out after sampling after growth 60 days.
2, field experiment proves the remediation efficiency of the restoring cadmium polluted agricultural land soil of beggar-ticks
It is acquired to the beggar-ticks planted in field trial cell, acquires beggar-ticks overground part, and in plant Bidens bipinnata
Grass seeds growing area delimit 4m at random2Cell, harvest fully after upper plant, are weighed, record, to calculate crop field biomass, measurement
Beggar-ticks overground part cadmium content and removal rate, are shown in Table 4；
4 field trial area beggar-ticks overground part yield (in terms of fresh weight) of table and Cd content (in terms of dry weight)
Note: content=Mean ± SD.
It is found by table 4, overground part Cd content average value is 10.94mgkg-1, maximum value is 14.34 mgkg-1,
Concentration coefficient average value is 4.16, and maximum value 5.94 passes through the several 4m delimited at random when gathering in beggar-ticks2Cell is received
After obtaining whole plants, weighing obtains 4m2The beggar-ticks average biomass of cell, contaminated area 18.01kg, maximum value are
19.52kg, measurement obtain beggar-ticks moisture content be 72.0% (table 4), thus calculate per acre beggar-ticks overground part biology
Measuring (fresh weight) is about 3003kg (18.01kg ÷ 4m2×667m2≈ 3003kg), maximum biomass (fresh weight) is about per acre
3258kg therefore deduces that one batch of beggar-ticks of every kind of 0.07 hectare, the amount of heavy metal in soil Cd can be taken away by beggar-ticks
(3003kg × 0.28 × 10.94mgkg about 9198.8mg-1≈ 9198.8), maximum value 13081.5mg, therefore plant per acre
Planting one batch to take away Cd in soil is about 9.2~13.1g, and polluted farmland soil Cd content is 2.66 mgkg-1, it is also contemplated that
The most effective part of arable layer is 0~10cm in farmland, then heavy metal contained by farmland topsoil (0~10cm) per acre
Cd is about 212.9g (667m2×10cm×1.2g·cm-3× 2.66mg·kg-1≈ 212.9g), every kind of one batch of Bidens bipinnata per acre
Grass can take away 4.3%~6.2% Cd in contaminated area soil, i.e. removal rate is 4.3%~6.2% (9.2g ÷ 212.9g
≈ 4.3%), survey region is middle subtropical zone monsoon region, and heat is high, and rainfall is big, and the sunshine-duration is long, meets the growth of beggar-ticks
Demand, therefore 3 batches of beggar-ticks can be planted every year, in the case where not adding activator, the removal of 3 batches of beggar-ticks is planted per acre
Rate is 12.9%~18.6%.
Claims (3)
1. a kind of method with the restoring cadmium polluted agricultural land soil of beggar-ticks, it is characterised in that specific steps are as follows:
Step 1: proving absorption of the beggar-ticks to Cd stress；
Step 2: proving operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering.
2. the method for the restoring cadmium polluted agricultural land soil of a kind of beggar-ticks according to claim 1, it is characterized in that the step
Rapid one proves absorption of the beggar-ticks to Cd stress, specifically includes: beggar-ticks seedling replanting being entered by Cd-polluted farmland soil, is adopted
With the mode of outdoor cultivation.
3. a kind of method with the restoring cadmium polluted agricultural land soil of beggar-ticks according to claim 1, it is characterized in that described
Step 2 proves operability of the beggar-ticks in actual Cd-polluted farmland soil remediation engineering, specifically includes: by beggar-ticks
Seed presses line-spacing 20cm, using (1.5kg mus of drilling method-1), the mode of field cultivation, soil of deep ploughing uniformly applies compound
Fertilizer is used as base manure, mixes and applies rear rotary tillage, periodically waters, and keeping field capacity is 65~80%, 60 days harvesting overground parts after maturation
Beggar-ticks then plants second batch three batches, repeats the above process, until repairing polluted farmland soil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111749254A (en) * | 2020-07-22 | 2020-10-09 | 湘潭大学 | Method for restoring side slope soil of subsidence area by converting open pit mining into underground mining |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147914A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Method for repairing cadmium pollution soil using with asteraeae plant |
| CN108526216A (en) * | 2018-04-24 | 2018-09-14 | 四川农业大学 | A method of improving bidens pilosa cadmium pollution repair ability |
-
2018
- 2018-11-27 CN CN201811425833.7A patent/CN109396165A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147914A (en) * | 2006-09-22 | 2008-03-26 | 中国科学院沈阳应用生态研究所 | Method for repairing cadmium pollution soil using with asteraeae plant |
| CN108526216A (en) * | 2018-04-24 | 2018-09-14 | 四川农业大学 | A method of improving bidens pilosa cadmium pollution repair ability |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111749254A (en) * | 2020-07-22 | 2020-10-09 | 湘潭大学 | Method for restoring side slope soil of subsidence area by converting open pit mining into underground mining |
| CN111749254B (en) * | 2020-07-22 | 2021-11-16 | 湘潭大学 | Method for restoring side slope soil of subsidence area by converting open pit mining into underground mining |
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