CN109394839A - One kind having hypoglycemic or effect for reducing blood fat roseapple seed extract and its preparation method and application - Google Patents
One kind having hypoglycemic or effect for reducing blood fat roseapple seed extract and its preparation method and application Download PDFInfo
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- 239000000284 extract Substances 0.000 title claims abstract description 128
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- 230000000694 effects Effects 0.000 title claims abstract description 40
- 230000002218 hypoglycaemic effect Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The present invention relates to Natural Medicine Chemistry technical fields, specifically disclose a kind of with hypoglycemic or effect for reducing blood fat roseapple seed extract and its preparation method and application.The preparation method comprises the following steps: taking roseapple seed, extracts to obtain roseapple seed alcohol extract with ethyl alcohol；Add water to be suspended roseapple seed alcohol extract, then extracted 1~3 time with organic solvent, obtain roseapple seed extract, that is, there is hypoglycemic or effect for reducing blood fat roseapple seed extract；The organic solvent is the mixed organic solvents that ethyl acetate and n-butanol form.The roseapple seed extract being prepared by this method is enriched a large amount of hypoglycemic and Effective Fractions Depressing Blood-lipids, and hypoglycemic and lipid-lowering effect is significant.
Description
Technical field
The present invention relates to Natural Medicine Chemistry technical fields, and in particular to one kind has hypoglycemic or effect for reducing blood fat Portugal
Seed extract and its preparation method and application.
Background technique
Diabetes are the metabolic diseases as caused by Different types of etiopathogenises characterized by chronic hyperglycemia, in diabetic's body
The metabolic disorder of sugar, fat, protein can lead to a series of chronic complicating diseases such as hypertension, renal lesions, vascular lesion, glycosuria
Disease has become the worldwide public health problem for seriously threatening human health.International Diabetes Federation (IDF) newest publication
8th edition " diabetes general view " (Diabetes Atlas) is pointed out, global about 4.25 hundred million adults suffer from diabetes within 2017, it is contemplated that arrive
2045, diabetic was likely to be breached 6.29 hundred million, there is within every 6 seconds 1 diabetic's death, and diabetes are died of in the whole world every year
Number be more than malaria, pulmonary tuberculosis and AIDS lethal number summation.China diabetic has surpassed 100,000,000, adult glycosuria
Disease and prediabetes illness rate are respectively 11.6% and 50.1%, are in high prevalence situation.Currently, clinically main useization
Drug treatment of diabetic is learned, it is strong, rapid-action to have the characteristics that hypoglycemic effect, but often lack whole harmony, simultaneously
There are toxic side effects after offer limited effectiveness, long-term administration larger, possible the disadvantages of causing and aggravating complication.Chinese herbal medicine is used in China
With a history of thousands of years in treatment diabetes, clinical experience is abundant, has many proved recipes and has obtained answering well in clinic
With.Recently research have indicated that Chinese herbal medicine can by too many levels, multiple target point reduce blood glucose, mitigate diabetes to body damage, control and
Delaying complications of diabetes, and effective component of chinese medicine has many advantages, such as that curative effect stabilization, small toxicity, adverse reaction is few, it is especially suitable
In prevention and treatment diabetic complication.Therefore, the Remedies for diabetes for screening and researching and developing high-efficiency low-toxicity from Chinese herbal medicine is still wound
One of the emphasis of novel drugs research and development, has significant importance and urgency.
Roseapple seed, Chinese medicine name are the seed of myrtle Portugal Syzygium jambos (L.) Alston.Point
It is distributed in the ground such as Fujian, Guangdong, Guangxi, Hainan, Guizhou, Yunnan, Taiwan.With invigorating the spleen, the effect of antidiarrheal.It is usually used in spleen deficiency to let out
It rushes down, protracted dysentery, diabetes.Although the prior art has and has hypoglycemic report to roseapple seed extract, but it is to roseapple seed
The research of middle effective component is not deep enough, the simple research only done after water or alcohol extracting in roseapple seed.And this field skill
Art personnel both know about, a large amount of chemical component contained in roseapple seed, then which ingredient or which active component have drop
Blood glucose effect, the prior art do not do in-depth study.And for roseapple seed, enriched how is prepared with blood sugar reducing component
Active component be one of research direction of those skilled in the art.
Summary of the invention
The technical problem to be solved by the invention is to provide one kind, and there is hypoglycemic or effect for reducing blood fat roseapple seed to extract
Object and preparation method thereof；The roseapple seed extract is enriched a large amount of hypoglycemic and Effective Fractions Depressing Blood-lipids, drops blood
Sugar and lipid-lowering effect are significant.
Above-mentioned technical problem to be solved by this invention, is achieved by the following technical programs:
A kind of preparation method with hypoglycemic or effect for reducing blood fat roseapple seed extract, it includes following steps:
Roseapple seed is taken, extracts to obtain roseapple seed alcohol extract with ethyl alcohol；
Add water to be suspended roseapple seed alcohol extract, is then extracted 1~3 time with organic solvent, obtain roseapple seed extract, i.e.,
With hypoglycemic or effect for reducing blood fat roseapple seed extract；It is mixed that the organic solvent is that ethyl acetate and n-butanol form
Close organic solvent.
Preferably, the organic solvent is that the mixing that 1~3:1 is formed by volume of ethyl acetate and n-butanol is organic molten
Agent.
Preferably, the ethyl alcohol extracts, and extracts using the ethyl alcohol that volume fraction is 95%.
Preferably, the described ethyl alcohol extraction refers to ethyl alcohol heating and refluxing extraction, the heating and refluxing extraction number is 1~
3 times, each extraction time is 1~2h, and the amount ratio of roseapple seed and ethyl alcohol is 1g:5~10mL.
Preferably, the preparation method further comprises silica gel column chromatography step, the silica gel column chromatography step tool
Body are as follows:
By silicagel column on the roseapple seed extract being prepared, first with volume ratio be 95~92:5~8 hexamethylene and
The mixed solvent of ethyl acetate composition elutes removal of impurities；It then is again the hexamethylene and acetic acid second of 88~85:12~15 with volume ratio
The mixed solvent elution of ester composition, and the elution position is collected, there is hypoglycemic or reducing blood lipid up to described after concentrate drying
Act on roseapple seed extract.
It is further preferred that the silica gel column chromatography step specifically:
By silicagel column on the roseapple seed extract being prepared, the hexamethylene and ethyl acetate for being first 93:7 with volume ratio
The mixed solvent of composition elutes removal of impurities；Then the mixed solvent of the hexamethylene and ethyl acetate composition that are again 87:13 with volume ratio
Elution, and the elution position is collected, up to described there is hypoglycemic or effect for reducing blood fat roseapple seed to extract after concentrate drying
Object.
It is further preferred that the silica gel column chromatography step specifically:
By silicagel column, the ring for being first 93:7 with the volume ratio of 2~4 times of column volumes on the roseapple seed extract being prepared
The mixed solvent of hexane and ethyl acetate composition elutes removal of impurities；Then the ring for being again 87:13 with the volume ratio of 4~6 times of column volumes
The mixed solvent elution of hexane and ethyl acetate composition, and the elution position is collected, there is drop up to described after concentrate drying
Blood glucose or effect for reducing blood fat roseapple seed extract.
Most preferably, the silica gel column chromatography step specifically:
By silicagel column, the hexamethylene for being first 93:7 with the volume ratio of 3 times of column volumes on the roseapple seed extract being prepared
The mixed solvent of alkane and ethyl acetate composition elutes removal of impurities；Then again with the volume ratio of 5 times of column volumes be 87:13 hexamethylene and
The mixed solvent elution of ethyl acetate composition, and collects the elution position, after concentrate drying up to it is described have it is hypoglycemic or
Effect for reducing blood fat roseapple seed extract.
The column volume refer to silica filler fill column after, from the bottom plate of column to the volume of silica filler deposition surface.
The present invention also provides a kind of prepared by the preparation method with hypoglycemic or effect for reducing blood fat Portugal kind
Seed extract.
The present invention also provides one kind by above-mentioned there is hypoglycemic or effect for reducing blood fat roseapple seed extract to prepare drug
Or the application in health care product.
The utility model has the advantages that the present invention provides a kind of completely new having is hypoglycemic or effect for reducing blood fat roseapple seed extract
Preparation method；The roseapple seed extract being prepared by this method is enriched a large amount of hypoglycemic and Effective Fractions Depressing Blood-lipids,
Its hypoglycemic and lipid-lowering effect is significant；This method is after ethyl alcohol extracts, using being mixed with by ethyl acetate and n-butanol
Solvent is extracted, and is tested by embodiment it can be seen that the extract obtained using mixed extractant solvent, hypoglycemic and drop
Blood fat is better than the extract obtained using independent ethyl acetate and extracting n-butyl alcohol；Further, the method is also
It, can be under the conditions of silica gel column chromatography of the present invention it can be seen from embodiment test including silica gel column chromatography step
One step concentration effect is superior hypoglycemic and Effective Fractions Depressing Blood-lipids；By contrast, under the conditions of other silica gel column chromatographies not
Can enrichment there is hypoglycemic or reducing blood lipid effective component, or be unable to concentration effect preferably and have hypoglycemic or reducing blood lipid have
Imitate ingredient.
Specific embodiment
The present invention is explained further below in conjunction with specific embodiment, but embodiment does not do any type of limit to the present invention
It is fixed.
The preparation of 1 roseapple seed extract of embodiment
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract, that is, has hypoglycemic or effect for reducing blood fat roseapple seed extract；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is that the mixing that 2:1 is formed by volume of ethyl acetate and n-butanol is organic molten
Agent；The amount ratio of the roseapple seed alcohol extract and water is 1g:20mL；In each extraction process the volume of mixed organic solvents with
The volume ratio of water is 1:1.
The preparation of 2 roseapple seed extract of embodiment
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract；
(3) by silicagel column, the ring for being first 93:7 with the volume ratio of 3 times of column volumes on the roseapple seed extract being prepared
The mixed solvent of hexane and ethyl acetate composition elutes removal of impurities；Then the hexamethylene for being again 87:13 with the volume ratio of 5 times of column volumes
It is eluted with the mixed solvent of ethyl acetate composition, and collects the elution position, up to described with hypoglycemic after concentrate drying
Or effect for reducing blood fat roseapple seed extract；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is that the mixing that 2:1 is formed by volume of ethyl acetate and n-butanol is organic molten
Agent；The amount ratio of the roseapple seed alcohol extract and water is 1g:20mL；In each extraction process the volume of mixed organic solvents with
The volume ratio of water is 1:1；
The mesh number of silica gel is 300 mesh in step (3) silicagel column, and the weight consumption of silica gel is roseapple seed extract weight
30 times.
The preparation of 1 roseapple seed extract of comparative example
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract, i.e. roseapple seed extract；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is ethyl acetate；The amount ratio of the roseapple seed alcohol extract and water is 1g:
20mL；The volume of organic solvent and the volume ratio of water are 1:1 in each extraction process.
Comparative example 1 the difference from embodiment 1 is that, only used ethyl acetate in 1 extraction process of comparative example, and embodiment
It is then using the mixed organic solvents being made of ethyl acetate and n-butanol in 1 extraction process.
The preparation of 2 roseapple seed extract of comparative example
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract, i.e. roseapple seed extract；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is n-butanol；The amount ratio of the roseapple seed alcohol extract and water is 1g:
20mL；The volume of organic solvent and the volume ratio of water are 1:1 in each extraction process.
Comparative example 1 the difference from embodiment 1 is that, only used n-butanol in 1 extraction process of comparative example, and embodiment 1
It is then using the mixed organic solvents being made of ethyl acetate and n-butanol in extraction process.
The preparation of 3 roseapple seed extract of comparative example
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract；
(3) by silicagel column, the ring for being first 93:7 with the volume ratio of 3 times of column volumes on the roseapple seed extract being prepared
The mixed solvent of hexane and ethyl acetate composition elutes removal of impurities；Then the hexamethylene for being again 80:20 with the volume ratio of 5 times of column volumes
It is eluted with the mixed solvent of ethyl acetate composition, and collects the elution position, up to roseapple seed extract after concentrate drying；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is that the mixing that 2:1 is formed by volume of ethyl acetate and n-butanol is organic molten
Agent；The amount ratio of the roseapple seed alcohol extract and water is 1g:20mL；In each extraction process the volume of mixed organic solvents with
The volume ratio of water is 1:1；
The mesh number of silica gel is 300 mesh in step (3) silicagel column, and the weight consumption of silica gel is roseapple seed extract weight
30 times.
Comparative example 3 and embodiment 2 the difference is that, the elution requirement of silica gel column chromatography is different.Comparative example 3 is first to use
The mixed solvent of hexamethylene and the ethyl acetate composition of 93:7 elutes removal of impurities；Then the hexamethylene and ethyl acetate of 80:20 are used again
The mixed solvent of composition elutes, and collects the elution position of 80:20；And embodiment 2 is then first to use the hexamethylene and acetic acid of 93:7
The mixed solvent of ethyl ester composition elutes removal of impurities；Then it is eluted again with the mixed solvent of the hexamethylene of 87:13 and ethyl acetate composition,
And collect the elution position of 87:13.Comparative example 3 is identical with the removal of impurities eluting solvent ratio of embodiment 2, and the eluting solvent eluted
Ratio is different, and elution has used bigger polar eluting solvent ratio.
The preparation of 4 roseapple seed extract of comparative example
(1) roseapple seed is taken, with ethyl alcohol heating and refluxing extraction 2 times, extracts 1.5h every time, merges simultaneously concentrated extracting solution, goes
Except after solvent roseapple seed alcohol extract；
(2) add water to be suspended roseapple seed alcohol extract, then extracted 2 times with organic solvent, combining extraction liquid concentration removal
Solvent obtains roseapple seed extract；
It (3) is first 85:15's with the volume ratio of 3 times of column volumes by silicagel column on the roseapple seed extract being prepared
The mixed solvent of hexamethylene and ethyl acetate composition elutes removal of impurities；Then the hexamethylene for being again 80:20 with the volume ratio of 5 times of column volumes
The mixed solvent elution of alkane and ethyl acetate composition, and the elution position is collected, up to the roseapple seed after concentrate drying
Extract；
The amount ratio of roseapple seed described in step (1) and ethyl alcohol is 1g:8mL；
Organic solvent described in step (2) is that the mixing that 2:1 is formed by volume of ethyl acetate and n-butanol is organic molten
Agent；The amount ratio of the roseapple seed alcohol extract and water is 1g:20mL；In each extraction process the volume of mixed organic solvents with
The volume ratio of water is 1:1；
The mesh number of silica gel is 300 mesh in step (3) silicagel column, and the weight consumption of silica gel is roseapple seed extract weight
30 times.
Comparative example 4 and embodiment 2 the difference is that, the elution requirement of silica gel column chromatography is different.Comparative example 3 is first to use
The mixed solvent of hexamethylene and the ethyl acetate composition of 85:15 elutes removal of impurities；Then the hexamethylene and ethyl acetate of 80:20 are used again
The mixed solvent of composition elutes, and collects the elution position of 80:20；And embodiment 2 is then first to use the hexamethylene and acetic acid of 93:7
The mixed solvent of ethyl ester composition elutes removal of impurities；Then it is eluted again with the mixed solvent of the hexamethylene of 87:13 and ethyl acetate composition,
And collect the elution position of 87:13.The removal of impurities eluting solvent ratio and eluting solvent ratio of comparative example 4 and embodiment 2 are different,
Bigger polar eluting solvent ratio is used.
The reducing blood sugar and blood fat effect assessment of 3 roseapple seed extract of embodiment
It takes male Kunming strain mice (KM), SPE grades, weight (20 ± 2) g, is purchased from Guangdong Province's Experimental Animal Center.It adapts to
Property feed 1 week after, randomly select 10 and be only used as blank group, feed conventional feed, remaining mouse feeds high glucose and high fat feed (feed
In containing 20% lard, 20% yolk powder, 10% sucrose, 2% cholesterol, 1% cholate and 47% conventional raise
Material).After 4 weeks, fasting 8h, naive mice is given 4.4 citrate buffer solution 40mg/kg of pH and is injected intraperitoneally 4 times, high glucose and high fat
Diet group mouse gives STZ solution (preparation of 4.4 citrate buffer solution of pH) 40mg/kg and is injected intraperitoneally 4 times, 1 time a day, continues
With same forage feed each group mouse.After STZ is injected 1 week, mouse tail vein takes blood, measures blood glucose value, blood glucose value is existed
11.1mmol/L's or more is set to Experimental diabetic model mouse.
It takes above-mentioned Experimental diabetic model mouse to be grouped at random, is divided into model group and experimental group is specifically grouped situation
See Tables 1 and 2, every group each 10；Set that normal mouse is cooked normal group and positive control drug melbine group compares simultaneously.
Experimental group carries out gastric infusion, and dosage is 200mg/ (kgd)；Model group and normal group stomach-filling physiological saline；It is positive right
Positive control drug melbine is given according to medicine melbine group, dosage is 200mg/ (kgd).Daily gastric infusion 1 time, continuously
4 weeks.Per weekend weighs in 1 time, adjusts dosage according to weight.It is surveyed after taking blood to survey fasting blood sugar and 4th week weekly
TG, TC, LDL-C, HDL-C value；Mouse fasting 12h before drawing blood；Concrete outcome is shown in Tables 1 and 2.
The experimental group difference administrations are as follows: experimental group 1 is given the roseapple seed that embodiment 1 is prepared and is extracted
Object；Experimental group 2 gives the roseapple seed extract that embodiment 2 is prepared；Experimental group 3 gives the Pu that comparative example 1 is prepared
Peach seeds extract；Experimental group 4 gives the roseapple seed extract that comparative example 2 is prepared；Experimental group 5 is given pair
The roseapple seed extract that ratio 3 is prepared；Experimental group 6 gives the roseapple seed extract that comparative example 4 is prepared.
Influence of the roseapple seed extract of the present invention of table 1 to diabetic mice fasting blood-glucose
By 1 data of table it can be seen that test group 1 (1 roseapple seed extract of embodiment) shows certain hypoglycemic effect,
This illustrates that roseapple seed extracts through ethyl alcohol of the present invention and has certain hypoglycemic effect after mixed extractant solvent.Test group 2 is (real
Apply 2 roseapple seed extract of example) its blood sugar decreasing effect has compared with experimental group 1 and is significantly promoted, and its blood sugar decreasing effect is wanted
Better than melbine group；This explanation roseapple seed further after silica gel column chromatography of the present invention, is enriched big
The blood sugar reducing component of amount, blood sugar decreasing effect is very significant, and is better than positive control drug melbine.
By experimental group 1 (1 roseapple seed extract of embodiment) and experimental group 3 (1 roseapple seed extract of comparative example), 4 (right
2 roseapple seed extract of ratio) it compares, it can be seen that the hypoglycemic data of experimental group 1 are better than the hypoglycemic of experimental group 3 and 4
Data；This explanation, using being extracted by the mixed organic solvents that ethyl acetate and n-butanol form, obtains in extraction step
The blood sugar decreasing effect of roseapple seed extract be better than the Portugal being individually obtained by extraction using ethyl acetate or n-butanol
Seed extract.
By experimental group 2 (2 roseapple seed extract of embodiment) and experimental group 5 (3 roseapple seed extract of comparative example), 6 (right
4 roseapple seed extract of ratio) it compares, it can be seen that the hypoglycemic data of experimental group 2 will be far superior to the drop of experimental group 5 and 6
Blood glucose level data；This explanation can be enriched to the drop blood of a large amount of excellent effect using silica gel column chromatography elution requirement of the present invention
Sugared ingredient, and the blood sugar reducing component with very good effect cannot be enriched with or cannot be enriched with using other elution requirements.
2. roseapple seed extract of table is to serum TG, TC, LDL-C, the influence of HDL-C
By 2 data of table it can be seen that test group 1 (1 roseapple seed extract of embodiment) shows certain effect for reducing blood fat,
This illustrates that roseapple seed extracts through ethyl alcohol of the present invention and has certain effect for reducing blood fat after mixed extractant solvent.Test group 2 is (real
Apply 2 roseapple seed extract of example) its blood sugar decreasing effect has compared with experimental group 1 and significantly promoted；This explanation Portugal
Seed further after silica gel column chromatography of the present invention, is enriched a large amount of component for reducing blood fat, lipid-lowering effect is very
Significantly.
By experimental group 1 (1 roseapple seed extract of embodiment) and experimental group 3 (1 roseapple seed extract of comparative example), 4 (right
2 roseapple seed extract of ratio) it compares and by experimental group 2 (2 roseapple seed extract of embodiment) and (comparative example 3 of experimental group 5
Roseapple seed extract), 6 (4 roseapple seed extracts of comparative example) compare, it can be seen that the reducing blood lipid data of experimental group 1 want excellent
In the reducing blood lipid data of experimental group 3 and 4；The reducing blood lipid data of experimental group 2 will be far superior to the reducing blood lipid data of experimental group 5 and 6.
This explanation uses in extraction step to be extracted by the mixed organic solvents that ethyl acetate and n-butanol form, obtained Portugal
The lipid-lowering effect of seed extract, which is better than, individually to be mentioned using the roseapple seed that ethyl acetate or n-butanol are obtained by extraction
Take object；The component for reducing blood fat of a large amount of excellent effect can be enriched to using silica gel column chromatography elution requirement of the present invention, and
The component for reducing blood fat with very good effect cannot be enriched with or cannot be enriched with using other elution requirements.
Claims (10)
1. a kind of preparation method with hypoglycemic or effect for reducing blood fat roseapple seed extract, which is characterized in that comprising such as
Lower step:
Roseapple seed is taken, extracts to obtain roseapple seed alcohol extract with ethyl alcohol；
Add water to be suspended roseapple seed alcohol extract, is then extracted 1~3 time with organic solvent, obtain roseapple seed extract, that is, have
Hypoglycemic or effect for reducing blood fat roseapple seed extract；
The organic solvent is the mixed organic solvents that ethyl acetate and n-butanol form.
2. preparation method according to claim 1, which is characterized in that the organic solvent is ethyl acetate and n-butanol
The mixed organic solvents of 1~3:1 composition by volume.
3. preparation method according to claim 1, which is characterized in that the ethyl alcohol extracts, using volume fraction
It is extracted for 95% ethyl alcohol.
4. preparation method according to claim 1, which is characterized in that the ethyl alcohol extraction refers to that ethyl alcohol is heated to reflux and mentions
It takes, the heating and refluxing extraction number is 1~3 time, and each extraction time is 1~2h, the amount ratio of roseapple seed and ethyl alcohol
For 1g:5~10mL.
5. preparation method according to claim 1, which is characterized in that it further comprise silica gel column chromatography step, it is described
Silica gel column chromatography step specifically:
It is first the hexamethylene and acetic acid of 95~92:5~8 with volume ratio by silicagel column on the roseapple seed extract being prepared
The mixed solvent of ethyl ester composition elutes removal of impurities；It then is again the hexamethylene and ethyl acetate group of 88~85:12~15 with volume ratio
At mixed solvent elution, and collect the elution position, there is hypoglycemic or effect for reducing blood fat up to described after concentrate drying
Roseapple seed extract.
6. preparation method according to claim 5, which is characterized in that the silica gel column chromatography step specifically:
By silicagel column on the roseapple seed extract being prepared, the hexamethylene and ethyl acetate for being first 93:7 with volume ratio are formed
Mixed solvent elute removal of impurities；Then the mixed solvent of the hexamethylene for being again 87:13 with volume ratio and ethyl acetate composition elutes,
And the elution position is collected, there is hypoglycemic or effect for reducing blood fat roseapple seed extract up to described after concentrate drying.
7. preparation method according to claim 5, which is characterized in that the silica gel column chromatography step specifically:
By silicagel column, the hexamethylene for being first 93:7 with the volume ratio of 2~4 times of column volumes on the roseapple seed extract being prepared
Removal of impurities is eluted with the mixed solvent of ethyl acetate composition；Then the hexamethylene for being again 87:13 with the volume ratio of 4~6 times of column volumes
It is eluted with the mixed solvent of ethyl acetate composition, and collects the elution position, up to described with hypoglycemic after concentrate drying
Or effect for reducing blood fat roseapple seed extract.
8. preparation method according to claim 7, which is characterized in that the silica gel column chromatography step specifically:
By silicagel column on the roseapple seed extract being prepared, first with the volume ratio of 3 times of column volumes be 93:7 hexamethylene and
The mixed solvent of ethyl acetate composition elutes removal of impurities；Then the hexamethylene and acetic acid for being again 87:13 with the volume ratio of 5 times of column volumes
The mixed solvent elution of ethyl ester composition, and the elution position is collected, there is hypoglycemic or drop blood up to described after concentrate drying
Rouge acts on roseapple seed extract.
9. what preparation method according to any one of claims 1 to 8 was prepared has hypoglycemic or effect for reducing blood fat Portugal kind
Seed extract.
10. as claimed in claim 9 there is hypoglycemic or effect for reducing blood fat roseapple seed extract to prepare drug or health care product
In application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050933A (en) * | 2019-04-16 | 2019-07-26 | 嘉应学院 | A kind of hypoglycemic solid beverage and preparation method thereof of the active component of fruit containing Portugal |
| CN113367166A (en) * | 2021-05-13 | 2021-09-10 | 海南大学 | Eugenia jambolana extract bactericide as well as preparation method and application thereof |
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2018
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Non-Patent Citations (2)
| Title |
|---|
| 张声源等: "蒲桃枝叶抑制α-葡萄糖苷酶活性部位及其化学成分研究", 《天然产物研究与开发》 * |
| 邓家刚等: "蒲桃仁提取物降血糖作用的实验研究 ", 《广西植物》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050933A (en) * | 2019-04-16 | 2019-07-26 | 嘉应学院 | A kind of hypoglycemic solid beverage and preparation method thereof of the active component of fruit containing Portugal |
| CN110050933B (en) * | 2019-04-16 | 2022-08-12 | 嘉应学院 | Hypoglycemic solid beverage containing effective parts of rose apple fruits and preparation method thereof |
| CN113367166A (en) * | 2021-05-13 | 2021-09-10 | 海南大学 | Eugenia jambolana extract bactericide as well as preparation method and application thereof |
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