CN105193877B - A kind of mystery fruit tree branch extract and its there is the application in hypoglycemic or effect for reducing blood fat drug or health care product in preparation - Google Patents
A kind of mystery fruit tree branch extract and its there is the application in hypoglycemic or effect for reducing blood fat drug or health care product in preparation Download PDFInfo
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- CN105193877B CN105193877B CN201510687319.0A CN201510687319A CN105193877B CN 105193877 B CN105193877 B CN 105193877B CN 201510687319 A CN201510687319 A CN 201510687319A CN 105193877 B CN105193877 B CN 105193877B
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Abstract
The present invention relates to Natural Medicine Chemistry technical fields, specifically disclose a kind of mysterious fruit tree branch extract and its application in the drug or health care product that preparation has reducing blood sugar and blood fat effect.The extract is prepared via a method which to obtain, and first crushes mysterious fruit tree branch, extracts to obtain extractive with organic solvent with organic solvent, then extracts to obtain using supercritical carbon dioxide method for extracting again.The extract has the hypoglycemic and effect for reducing blood fat of highly significant, and can be used for preparing has hypoglycemic or effect for reducing blood fat drug or health care product.
Description
Technical field
The present invention relates to Natural Medicine Chemistry technical fields, and in particular to a kind of mystery and its is being prepared fruit tree branch extract
With the application in hypoglycemic or effect for reducing blood fat drug or health care product.
Background technique
Miracle fruit, Classification system are Synsepalum dulcificum Daniell, are typical heat band evergreen shrubs,
Source area is in West Africa, Ghana, one band of the Congo.In the 1960s, Republic of Ghana is mind when Premier Zhou Enlai accesses to West Africa
Secret fruit gives premier Zhou as state's gift.Hereafter, miracle fruit starts to cultivate in China.
Patent CN102743421A discloses a kind of miracle fruit fruit tree branch leaf extract, it was recently reported that its with anti-oxidant and
Antigout effect.But it there is no the reducing blood sugar and blood fat effect for specifically reporting mysterious fruit tree branch, more without utilizing the modernization of Chinese medicine
Technology prepares the report of active component, and those skilled in the art are for hypoglycemic, reducing blood lipid effective portion in mysterious fruit tree branch
Position and effective component are also indefinite, and how preparing, there is fine hypoglycemic, lipid-lowering effect active component to still need to grind
Study carefully exploration.
Summary of the invention
The technical problem to be solved by the present invention is in order to overcome exploitation of the prior art to mysterious fruit tree branch active component
Deficiency provides a kind of mysterious fruit tree branch extract.The extract has the hypoglycemic and effect for reducing blood fat of highly significant, can be with
It is used to prepare with hypoglycemic or effect for reducing blood fat drug or health care product.
Above-mentioned technical problem to be solved by this invention, is achieved by the following technical programs:
A kind of mystery fruit tree branch extract, first crushes mysterious fruit tree branch, with organic solvent extract organic solvent mentions
Object is taken, then extracts to obtain using supercritical carbon dioxide method for extracting again.
Preferably, the organic solvent is ethyl alcohol, the supercritical carbon dioxide method for extracting are as follows: by mysterious fruit tree
Branch extractive with organic solvent is placed in supercritical carbon dioxide extracting instrument, and the pressure controlled in supercritical carbon dioxide extracting instrument is
30~40 megapascal, temperature are 40~50 DEG C, and flow velocity is 20~30 ls/h, and extraction time is 1~4 hour.
There is better hypoglycemic and lipid-lowering effect active component in order to obtain, the mysterious fruit tree branch is through super
After critical carbon dioxide extracting process extracts, then it is enriched with silica gel column chromatography.
Preferably, the silica gel column chromatography comprises the following steps: first by mysterious fruit tree branch through overcritical titanium dioxide
Silicagel column on the extract that carbon extracting process obtains after extracting, the hexamethylene for being then 99:1~93:7 with volume ratio: acetone is mixed
Bonding solvent elution, then the hexamethylene for being 88:12~90:10 with volume ratio: acetone mixed solvent elution, collected volume ratio are 88:
The hexamethylene of 12~90:10: acetone mixed solvent elutes position, is concentrated, is drying to obtain.
It is highly preferred that the silica gel column chromatography comprises the following steps: first by mysterious fruit tree branch through overcritical dioxy
Change silicagel column on the extract obtained after carbon extracting process extracts, the hexamethylene for being then 93:7 with volume ratio: acetone mixing is molten
Agent elution, then the hexamethylene for being 90:10 with volume ratio: acetone mixed solvent elution, collected volume is than the hexamethylene for 90:10:
Acetone mixed solvent elutes position, is concentrated, is drying to obtain.
Preferably, the silica gel dosage is the extract weight obtained after supercritical carbon dioxide method for extracting extracts
10~30 times；The mesh number of the silica gel is 200~300 mesh.
Preferably, the hexamethylene: the usage amount of acetone mixed solvent is 3~8 times of silicagel column column volume.
It is highly preferred that the silica gel column chromatography comprises the following steps: first by mysterious fruit tree branch through overcritical dioxy
The silicagel column of 10~30 times of extract weight of silica gel is housed on the extract that change carbon extracting process obtains after extracting, then uses body
Product is than the hexamethylene for 99:1~93:7: acetone mixed solvent elution, and the dosage of mixed solvent is the 3~5 of silicagel column column volume
Times；The hexamethylene for being again 90:10 with volume ratio: acetone mixed solvent elution, the dosage of mixed solvent are the 5 of silicagel column column volume
~8 times；Collected volume is than the hexamethylene for 90:10: acetone mixed solvent elutes position, is concentrated, is drying to obtain.
The mysterious fruit tree branch extract has the application in hypoglycemic or effect for reducing blood fat drug in preparation.
The mysterious fruit tree branch extract has the application in hypoglycemic or effect for reducing blood fat health care product in preparation.
The utility model has the advantages that the present invention provides a kind of miracle fruits that the one kind being prepared using modernization of Chinese medicine technology is completely new
Branch extract, the experimental data in embodiment show, reducing blood lipid and hypoglycemic effect with highly significant.
Specific embodiment
The present invention is explained further below in conjunction with specific embodiment, but embodiment does not do any type of limit to the present invention
It is fixed.
Embodiment 1
It weighs the dry mysterious fruit tree branch of 1kg to crush, then with ethyl alcohol heating and refluxing extraction 2 times of 5kg, each 1.5h, closes
And extracting solution, it is concentrated to give mysterious fruit tree branch ethanol extract；Mysterious fruit tree branch ethanol extract is placed in supercritical CO again2Extraction
In instrument, controlling the pressure in supercritical carbon dioxide extracting instrument is 30 megapascal, and temperature is 40 DEG C, and flow velocity is 20 ls/h, is extracted
Time is 2 hours, obtains mysterious fruit tree branch supercritical CO2Extract 95.7g.
Embodiment 2
It weighs the dry mysterious fruit tree branch of 1kg to crush, then with ethyl alcohol heating and refluxing extraction 2 times of 5kg, each 1.5h, closes
And extracting solution, it is concentrated to give mysterious fruit tree branch ethanol extract；Mysterious fruit tree branch ethanol extract is placed in supercritical CO again2Extraction
In instrument, controlling the pressure in supercritical carbon dioxide extracting instrument is 40 megapascal, and temperature is 50 DEG C, and flow velocity is 30 ls/h, is extracted
Time is 2 hours, obtains mysterious fruit tree branch supercritical CO2Extract 90.3g.
Embodiment 3
Extract 40g obtained in embodiment 1 is weighed, after being dissolved with ethyl acetate, is mixed with 40g silica gel (100~200 mesh)
Sample separately takes 800g silica gel (200~300 mesh) dry column-packing, and sample is packed on column, is first 93 with the volume ratio of 3 times of column volumes:
7 hexamethylene: acetone mixed solvent elution；The hexamethylene for being again 90:10 with the volume ratio of 8 times of column volumes: acetone mixed solvent
Elution；Collected volume is than the hexamethylene for 90:10: acetone mixed solvent elutes position, concentration, dried extract 8.8g.
Embodiment 4
Extract 40g obtained in embodiment 1 is weighed, after being dissolved with ethyl acetate, is mixed with 40g silica gel (100~200 mesh)
Sample separately takes 800g silica gel (200~300 mesh) dry column-packing, and sample is packed on column, is first 99 with the volume ratio of 3 times of column volumes:
1 hexamethylene: acetone mixed solvent elution；The hexamethylene for being again 88:12 with the volume ratio of 8 times of column volumes: acetone mixed solvent
Elution；Collected volume is than the hexamethylene for 88:12: acetone mixed solvent elutes position, concentration, dried extract 9.6g.
5 lipid-lowering test of embodiment
SD rat 70 are taken, half male and half female is divided into normal group of (feeding normal diet), high lipid food group, experimental group 1 and (implements
The extract that example 1 is prepared), experimental group 2 (extract that embodiment 2 is prepared), (embodiment 3 is prepared experimental group 3
Extract), experimental group 4 (extract that embodiment 4 is prepared), every group of positive controls (Lovastatin) be 10,
High lipid food group, experimental group and positive controls feed high lipid food, and in addition experimental group 1,2 is simultaneously daily by 20mg/kg's
Amount feeds the extract that corresponding embodiment is prepared, and experimental group 3,4 feeds corresponding embodiment preparation by the amount of 3mg/kg daily simultaneously
Obtained extract, the amount that positive controls press 3mg/kg daily feed Lovastatin.
Each group rat is fed by group, free water after successive administration 35 days, puts to death animal, and abdominal vein takes blood, measures
Blood lipid.Serum total cholesterol, triglycerides, high density rouge are measured on automatic clinical chemistry analyzer with enzyme process, Precipitation Determination
Protein cholesterol and low density lipoprotein cholesterol content.Experimental result is as shown in table 1.
The extract lipid-lowering test of the present invention of table 1
Compared with chow diet group, #P < 0.05, ##P < 0.01；Compared with high lipid food group, P < 0.01 * P < 0.05, * *.
From table 1 it follows that TC, TG and the LDL-C concentration of high lipid food group rat blood serum are all remarkably higher than normal feeding
Material group (P < 0.01), HDL-C concentration illustrate to model successfully lower than chow diet group (P < 0.05).Experimental group 1,2 rats serum
TC, TG and LDL-C concentration are significantly lower than high lipid food group (P < 0.01)；The concentration of HDL-C be apparently higher than high lipid food group (P <
0.05), illustrate that the mysterious fruit tree branch extract obtained through supercritical extract has apparent effect for reducing blood fat.May be used also from table 1
To find out, experimental group 3,4 rats serum TC, TG and LDL-C concentration reduces to become apparent, with positive control drug Lovastatin
Quite, this also illustrates the mysterious fruit tree branch that mysterious fruit tree branch obtains after after supercritical extract again by silica gel column chromatography technology
The lipid-lowering effect of extract further increases, and effect is more significant.In addition, it can also be seen that the use of experimental group 1,2 from table 1
Amount is 20mg/kg, and compared to for extract, dosage is quite few, this illustrates that present invention extraction has obtained mysterious fruit tree branch and had
The essence position of effect for reducing blood fat.More surprisingly the dosage of experimental group 3,4 is that 3mg reaches as Western medicine Lovastatin dosage
A small amount of efficient effect is arrived.
The hypoglycemic experiment of embodiment 6
60 mouse (7 week old male ICR mouse) is taken, weight 22-25g, in addition to 10 are only used as Normal group, remaining is small
100mg/kg streptozotocin solution is injected intraperitoneally in mouse, and blood glucose value is surveyed after 72 hours, and 11.1mmol/L or more person tests,
It is randomly divided into 5 groups, it may be assumed that model group and experimental group 1 (extract that embodiment 1 is prepared), (embodiment 2 is prepared into experimental group 2
To extract), experimental group 3 (extract that embodiment 3 is prepared), (extraction that embodiment 4 is prepared of experimental group 4
Object).Every group 10, (experimental group 1,2 daily administration dosage are 20mg/kg, experimental group 3,4 daily administrations for daily gastric infusion 1 time
Dosage is 3mg/kg), successive administration 30d, every 7d weighs 1 time, according to weight adjustment dosage；Respectively at the 10th, 20,30d
After last stomach-filling 2h (fasting 12h), mouse tail takes blood to survey blood glucose value.Equivalent is given in Normal group and model control group stomach-filling
Physiological saline (10ml/kg).Experimental result is as described in Table 2:
The 2 hypoglycemic experiment of extract of the present invention of table
Compared with normal group, #P < 0.05, ##P < 0.01；Compared with model group, P < 0.01 * P < 0.05, * *.
From Table 2, it can be seen that model group mouse, compared with Normal group, blood glucose level is significantly raised, illustrate chain urea
Assistant rhzomorph causes diabetic mouse model success.Extract prepared by embodiment 1, the 2 intracorporal blood glucose of mouse after 10d is administered
Content significantly reduces, and upon administration after 20d and 30d, blood-sugar content is further decreased.Illustrate extract prepared by embodiment 1,2
With significant blood sugar decreasing effect.Extract described in embodiment 3,4 is in embodiment 1,2 through carbon dioxide supercritical extraction
On the basis of the extract that is further refining to obtain with silica gel column chromatography technology, as can be seen from Table 2, blood sugar decreasing effect compared with
The extract that embodiment 1,2 is prepared further increases.In addition, it can also be seen that embodiment 1,2 is prepared from table 2
The dosage of extract be 20mg/kg, compared to for extract, dosage is quite few, illustrates that the present invention extracts and has obtained mystery
Fruit tree branch has the essence position of hypoglycemic effect.More surprisingly the dosage of experimental group 3,4 is 3mg, with Western medicine Lovastatin
Dosage is the same, this illustrates that present invention extraction has obtained mysterious branch extract and reached a small amount of efficient effect.
Claims (5)
1. a kind of mystery fruit tree branch extract has the application in hypoglycemic or effect for reducing blood fat drug or health care product in preparation；
It is characterized in that,
The mysterious fruit tree branch extract is prepared via a method which to obtain, and first crushes mysterious fruit tree branch, with organic molten
Extractive with organic solvent is extracted to obtain in agent, is then extracted again using supercritical carbon dioxide method for extracting；Silica gel column chromatography is used again
Enrichment；
The organic solvent is ethyl alcohol, the supercritical carbon dioxide method for extracting are as follows: by mysterious fruit tree branch organic solvent
Extract is placed in supercritical carbon dioxide extracting instrument, and controlling the pressure in supercritical carbon dioxide extracting instrument is 30 ~ 40,000,000
Pa, temperature are 40 ~ 50 DEG C, and flow velocity is 20 ~ 30 ls/h, and extraction time is 1~4 hour；
The silica gel column chromatography comprises the following steps: first mentioning mysterious fruit tree branch through supercritical carbon dioxide method for extracting
Silicagel column on the extract obtained after taking, the hexamethylene for being then 99:1 ~ 93:7 with volume ratio: acetone mixed solvent elution, then
The hexamethylene for being 88:12 ~ 90:10 with volume ratio: acetone mixed solvent elution, collected volume ratio are the hexamethylene of 88:12 ~ 90:10
Alkane: acetone mixed solvent elutes position, is concentrated, is drying to obtain.
2. application according to claim 1, which is characterized in that the silica gel column chromatography comprises the following steps: first
Then silicagel column on the extract that mysterious fruit tree branch is obtained after supercritical carbon dioxide method for extracting extracts is with volume ratio
The hexamethylene of 93:7: acetone mixed solvent elution, then the hexamethylene for being 90:10 with volume ratio: acetone mixed solvent elution is collected
Volume ratio is the hexamethylene of 90:10: acetone mixed solvent elutes position, is concentrated, is drying to obtain.
3. application according to claim 1, which is characterized in that the silica gel dosage is to extract through supercritical carbon dioxide
10 ~ 30 times of the extract weight obtained after taking method to extract；The mesh number of the silica gel is 200 ~ 300 mesh.
4. application according to claim 1, which is characterized in that hexamethylene: the usage amount of acetone mixed solvent is silica gel
3 ~ 8 times of column column volume.
5. application according to claim 1, which is characterized in that the silica gel column chromatography comprises the following steps: first
Extract weight 10 ~ 30 is housed on the extract that first mysterious fruit tree branch is obtained after supercritical carbon dioxide method for extracting extracts
The silicagel column of silica gel again, the hexamethylene for being then 99:1 ~ 93:7 with volume ratio: acetone mixed solvent elution, mixed solvent
Dosage is 3 ~ 5 times of silicagel column column volume；The hexamethylene for being again 90:10 with volume ratio: acetone mixed solvent elution, mixed solvent
Dosage be 5 ~ 8 times of silicagel column column volume；Collected volume is than the hexamethylene for 90:10: acetone mixed solvent elutes position, dense
It contracts, be drying to obtain.
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| CN201510687319.0A CN105193877B (en) | 2015-10-22 | 2015-10-22 | A kind of mystery fruit tree branch extract and its there is the application in hypoglycemic or effect for reducing blood fat drug or health care product in preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743421A (en) * | 2012-07-12 | 2012-10-24 | 万福群 | Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102743421A (en) * | 2012-07-12 | 2012-10-24 | 万福群 | Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract |
Non-Patent Citations (2)
| Title |
|---|
| 神秘果功能成分及开发利用研究;卢圣楼等;《食品与机械》;20130729;第29卷(第05期);256-260 |
| 神秘果及其复合物对四氧嘧啶糖尿病小鼠降血糖作用;郭刚军等;《热带农业科技》;20121215;第27卷(第04期);64-66 |
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Denomination of invention: The invention relates to a mysterious fruit tree branch extract and its application in the preparation of drugs or health products with hypoglycemic or hypolipidemic effects Effective date of registration: 20211209 Granted publication date: 20190702 Pledgee: Taishan Branch of China Construction Bank Co.,Ltd. Pledgor: GUANGDONG LONGFREE BIOLOGY CO.,LTD. Registration number: Y2021980014647 |
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