CN109385489A - A kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening - Google Patents
A kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening Download PDFInfo
- Publication number
- CN109385489A CN109385489A CN201811592821.3A CN201811592821A CN109385489A CN 109385489 A CN109385489 A CN 109385489A CN 201811592821 A CN201811592821 A CN 201811592821A CN 109385489 A CN109385489 A CN 109385489A
- Authority
- CN
- China
- Prior art keywords
- rice
- ossut3
- gene
- rice germplasm
- sut3
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening, including rice germplasm to be measured extract total DNA, with primer SUT3-5 ' utrF and SUT3-5 ' utrR expand, amplified production with 2.5% Ago-Gel, ethidium bromide staining, gel imaging system are taken pictures；The pcr amplification product of rice germplasm to be measured is 743bp band, then the rice germplasm is the rice germplasm of OsSUT3 gene a large amount expression；The pcr amplification product of rice germplasm to be measured is 712bp band, then the rice germplasm is the rice germplasm of OsSUT3 gene low amounts expression.Any one period of the present invention in rice growth, the height that accurately can be quickly identified rice germplasm material OsSUT3 gene expression amount using minim DNA sample is developed mark with the closely related InDel of rice sucrose transporter gene OsSUT3 expression quantity for the first time.
Description
Technical field
The invention belongs to crops technical field of molecular biology, specifically, being related to screening rice sucrose transport protein
Encoding gene OsSUT3 a large amount expression quantity rice germplasm InDel molecular labeling and PCR method.
Background technique
The storage (library) of the transport (stream) of organic matter and photosynthesis (source) and photosynthate weight on an equal basis in higher plant body
It wants, is the basis of plant growth and development.Sucrose is the base of synthetic starch as most important disaccharides in photosynthesis assimilation product
This raw material, at the same be also plant in photosynthate prevailing traffic form (Zimmermann and Ziegler,
1975；Ohshima et al.,1990；Rosa et al.,2009).Sucrose the loading of bast and unloading require by
Transmembrane protein passes through vascular bundle sheath cell or phloem parenchyma cell, and the albumen for executing transmembrane sucrose transport mainly has SWEET sugared
Transport protein (Braun, 2012；Chen et al.,2012；Eom et al., 2015), sucrose transporter (sucrose
transporters or carriers,SUTs or SUCs)(Aoki et al.,2003；Sauer, 2007) etc..Sucrose turns
It transports albumen (SUTs) and passes through sucrose/H+Collaboration in the same direction transport complete sucrose in the intracorporal transhipment of plant.In addition, SUTs is to coordinate
The important protein molecular of non-plant sucrose, Carbon storage plays vital work in the energy distribution of each growth phase of plant
With (Wang et al., 2015).The SUTs gene for encoding this albumen is a big family, all has one in each plant
The SUTs gene of series inhibits while cooperating between these SUTs genes there is also mutual, the common life for coordinating plant
Reason metabolism and growth and development (Lima et al., 2010).
Rice is the important cereal crops in China, and it is always to need to solve in China's agricultural production that its yield, which is continuously improved,
Major issue.For rice, the transhipment and distribution of sucrose are to determine the key factor of yield height and quality quality.Rice
Sucrose transporter gene family coding is made of 5 SUTs genes respectively, be the key gene of rice transhipment sucrose,
The expression of its a large amount will greatly promote sucrose conevying efficiency, to promote the growth of rice yield.OsSUT3 is OsSUTs gene man
Important member in race not only coordinates the transhipment of sucrose in rice, has an effect on starch during the development and pollen maturation of pollen
Synthesis (Hirose et al., 2010).Therefore, the high of OsSUT3 gene expresses the carbon water for being conducive to rice in rice plant
The transhipment of compound and the normal development of pollen, lay the foundation for rice high yield.Thus construct a kind of accurate, quickly screening
The method of the rice germplasm of OsSUT3 gene high expression is necessary.
Rice molecular biological study and the development of PCR molecular marking technique are the water for establishing the expression of OsSUT3 gene a large amount
The method that seed rice matter is accurately quickly screened has established reliable theory and technology basis.The present invention is based on us to OsSUT3 gene table
Up to the research of regulatory factor, the discovery one cis- enhancing for being located at the controllable OsSUT3 gene expression amount of OsSUT3 upstream region of gene because
Son, the enhancement factor are placed exactly in one InDel sequence, which can be obtained by PCR amplification.Therefore,
We are searched OsSUT3 gene and are risen by NCBI (https: //www.ncbi.nlm.nih.gov/) rice genome database
The base sequence of beginning codon (ATG) upstream 1000bp and downstream 500bp, total 1500bp；With primer-design software Primer
5.0 designs include the specific primer SUT3-5 ' utr of OsSUT3 gene upstream sequence.Then using round pcr to the InDel
Label amplification, so that it may the height of the accurate OsSUT3 gene expression amount for quickly identifying rice germplasm material.
Bibliography
Zimmermann,M,Ziegler,H.List of sugars and sugar alcohols in sieve-
tube exudates.In:Zimmermann,M.,Milburn,H.(Eds.),Encyclopedia of Plant
Physiology,NS,vol.1.Springer,New York.1975:480-503.
Ohshima,T,Hayashi,H,Chino,M.Collection and chemical composition of
pure phloem sap from Zea mays L.
Plant Cell Physiol.1990,31:735-737.
Rosa M.,Prado C.,Podazza G.,et al.Soluble sugars--metabolism,sensing
and abiotic stress:a complex network in the life of plants.Plant Signal
Behav.2009,4(5):388-393
Braun DM.SWEET！The pathway is complete.Science.2012,335:173-174.
Chen LQ,Qu XQ,Hou BH et al.Sucrose efflux mediated by SWEET proteins
as a key step for phloem transport.
Science.2012,335(6065):207-211.
Eom JS,Chen LQ,Sosso D et al.SWEETs,transporters for intracellular
and intercellular sugar translocation.
Curr Opin Plant Biol.2015,25:53-62.
Aoki N.,Hirose T.,Scofield G.N.,et al.The sucrose transporter gene
family in rice.Plant and Cell Physiology.
2003.44(3):223-232.
Sauer N.Molecular physiology of higher plant sucrose
transporters.FEBS Lett.2007,581(12):2309-2317.
Wang L,Lu Q,Wen X et al.Enhanced Sucrose Loading Improves Rice Yield
by Increasing Grain Size.Plant Physiol.2015,169(4):2848-2862.
Lima J.D.,Chob J.,Parkc Y.,et al.Sucrose transport from source to
sink seeds in rice.Physiologia Plantarum.
2010,126(4):572-584.
Hirose,T.,Zhang,Z.,Miyao,A.,Hirochika,H.,Ohsugi,R.,Terao,T.2010,
Disruption of a gene for rice sucrose transporter,OsSUT1,impairs pollen
function but pollen maturation is unaffected,J Exp Bot.,61,3639-46.
Summary of the invention
The present invention provide it is a kind of it is easy to operate, amount of samples is few, and any period of rice growth can be fast
Fast accurate identification rice germplasm material OsSUT3 gene expression amount height PCR method and its specific primer.It, can by the method
The high rice germplasm of accurate screening sucrose transfer efficiency improves the yield and quality to High-yield Rice Breeding is promoted with important meaning
Justice.
A kind of specific primer SUT3-5 ' utr of screening OsSUT3 gene a large amount expression rice germplasm provided by the invention,
It is made of primer SUT3-5 ' utrF and primer SUT3-5 ' utrR, the base sequence of primer SUT3-5 ' utrF such as SEQ ID NO:1
Shown, the base sequence of primer SUT3-5 ' utrR is as shown in SEQ ID NO:2.
A kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening provided by the invention, comprising the following steps:
(1) total DNA of rice germplasm to be measured is extracted；
(2) amplification of DNA target fragment is carried out in PCR instrument with above-mentioned specific primer SUT3-5 ' utr；
(3) after the completion of PCR amplification, 5 μ L amplified productions are taken, it is small in voltage 60V electrophoresis 2 using 2.5% Ago-Gel
When after ethidium bromide staining, take pictures under gel imaging system；
(4) pcr amplification product of rice germplasm to be measured is 743bp band, then shows that the rice germplasm is OsSUT3 gene
The rice germplasm of a large amount expression；The pcr amplification product of rice germplasm to be measured is 712bp band, then shows that the rice germplasm is
The rice germplasm (Fig. 1) of OsSUT3 gene low amounts expression.
Compared with prior art, main innovation of the invention point and beneficial effect are:
It can be accurate using minim DNA sample by Standard PCR technology in any one period of rice growth
The height for quickly identifying rice germplasm material OsSUT3 gene expression amount exempts fluorescence real-time quantitative PCR measurement generally used now
Many limitations possessed by the method for gene expression, such as fluorescence real-time quantitative PCR method generally used now will be in specific fertilities
Not only the time-consuming but also work of consumptive material such as period (gene expression peak) sampling, extraction RNA.It develops for the first time and transports base with rice sucrose
Because the closely related InDel of OsSUT3 expression quantity marks (specific primer SUT3-5 ' utr), provided just for Rice molecular breeding
Prompt approach.
In rice high yield, superior rice breeding process, can rice growth each period from the hereditary object of rice
Matter DNA inherently can huge discharge screen the high rice material of OsSUT3 gene expression amount, be not required to until rice Os SUT3 gene expression
The higher pustulation period is measured, can greatly shorten the time for screening high OsSUT3 gene expression amount germplasm, germplasm can also be greatly reduced
The workload of screening has many advantages, such as easy to operate, accurate, quick.
It is the base sequence of primer SUT3-5 ' utrF shown in SEQ ID NO:1 in sequence table.
It is the base sequence of primer SUT3-5 ' utrR shown in SEQ ID NO:2 in sequence table.
Detailed description of the invention
Fig. 1: the pcr amplification product and its OsSUT3 gene expression amount of specific primer OsSUT3-5 ' utr in rice varieties
Detection.M is DNA molecular amount standard.Swimming lane 1-4 indicates different rice, 1:93-11,2: OryzasativaLcv.Nipponbare, 3: Niu Bagu, 4: big
Fragrant paddy, the molecular weight of the PCR fragment of swimming lane 1-2 and 3-4 are respectively 743bp and 712bp, and top is the OsSUT3 of corresponding kind
The fold differences of the relative expression quantity of gene and the expression quantity of the kind with 743bp and 712bp segment.Rice varieties
The measurement of OsSUT3 gene expression amount is used by the blade total serum IgE before extracting Rice FloweringPremix EX TaqTMⅡ
(Tli RNaseH Plus) kit (Takara, Dalian, China) kit, passes through quantitative fluorescent PCR (qRT-PCR) method
Detection, each kind carry out 3 biology replications.
Fig. 2: the PCR identification of different rice material OsSUT3 expression quantity levels.Specific primer OsSUT3-5 ' utr PCR
The electrophoresis detection of amplified production is as a result, M is DNA molecular amount standard.The pcr amplified fragment of swimming lane 1-3,5-7,9-11 rice germplasm
Molecular weight be 743bp, illustrate these rice germplasms be OsSUT3 gene a large amount expression rice germplasm；4th and the 8th swimming lane
Pcr amplification product molecular weight be 712bp, illustrate two rice germplasms be OsSUT3 gene low amounts expression rice germplasm.Swimming
Road 1-11 is different rice, 1:93-11；2: OryzasativaLcv.Nipponbare；3: June paddy；4: big perfume paddy；5: climbing agriculture 1；6: Yongning little Bai Gu；
7: A Chuche；8: Niu Bagu；9: eight treasures (choice ingredients of certain special dishes) paddy；10: cold water paddy；11: big locust paddy.
Specific embodiment
The present invention is further illustrated with reference to embodiments, without specified otherwise is conventional method in embodiment.
One, material therefor is tested
Table 1 is for trying rice varieties
In table 1 all materials to be tested can from applicant Yunnan Prov Agriculture University from the applying date in 20 years to the public
It provides, Yunnan Prov Agriculture University address: Yunnan Province, Panlong District, the Kunming source Feng road 452, postcode: 650201.
1.93-11 open source literature: Zhang GH et al., LSCHL4from Japonica Cultivar, which is
allelic to NAL1,increases yield of indica super rice 93-11,Molecular Plant,
2014,7(8),1350-1364.
2. OryzasativaLcv.Nipponbare open source literature: Deng Y et al., Detection and correction of assembly
errors of rice Nipponbare reference sequence,Plant Biol(Stuttg),2014,16(3),
643-650.
3. paddy open source literature in June: the comparison of the photosynthesis and chlorophyll content of Li Mingqi etc., wild rice and cultivated rice
Research, plant physiology journal, 1984,4,333-338.
4. big perfume paddy, cold water paddy, big locust paddy open source literature: pretty swallow of bandit etc., the old kind of Xishuangbanna of China rice are being educated
The genetic diversity in the property site restoring gene Rf-1, Molecular Plant Breeding, 2009,7 (4), 653-660.
5. climbing No. 1 open source literature of agriculture: Li Song etc., rice cold hardness evaluation and its physiological index determining, Yunnan University's journal
Natural science edition, 1990,3,65.
6. Yongning little Bai Gu, A Chuche, Niu Bagu open source literature: Tan Yaling etc., height above sea level are raw to different rice varieties
Long influence research, seed, 2009,28 (7), 27-30.
7. eight treasures (choice ingredients of certain special dishes) paddy open source literature: Sun Yan etc., application of the Candidate Disease Resistant Genes in rice varieties Study on Diversity, China
Agricultural sciences, 2005,38 (11), 2227-2232.
Two, for the Genome DNA extraction of examination rice varieties
It is extracted from fresh rice seedlings (four leaf stage) using the CTAB method (Rogers and Bendich, 1985) of improvement
Total DNA.Method is as follows:
1) it takes about 0.5g four leaf stage rice material to be put into 1.5mL centrifuge tube, liquid nitrogen frozen grinding is added, after grinding
Powder in 2 × CTAB buffer, 600 μ L is added, be put into 45min in 65 DEG C of water-baths, mixed every 15min primary.
2) it takes out centrifuge tube and isometric phenol/chloroform/isoamyl alcohol (volume ratio 25:24:1) is added, after being mixed by inversion
10000rpm is centrifuged 10min.
3) pipette tips for spending point are carefully removed from upper strata aqueous phase liquid into the new centrifuge tube of 1.5mL, and 600 μ L phenol/chlorine is added
Imitative/isoamyl alcohol (volume ratio 25:24:1), is mixed by inversion rear 10000rpm, 10min.
4) carefully the dehydrated alcohol and 1/10 of 2-3 times of volume ice pre-cooling is added into new centrifuge tube in transfer upper strata aqueous phase
The 3M NaAC (sodium acetate) (pH5.2) of volume, centrifuge tube is placed in -20 DEG C of refrigerators and freezes 10-20min, until there is cotton-shaped sink
It forms sediment and generates.
5) choose flocculent deposit with the toothpick of sterilizing, and with 70% ethanol washing 2 times, suck ethyl alcohol simultaneously with the filter paper of sterilizing
Naturally dry makes ethyl alcohol sufficiently volatilize.
6) the TE solution dissolving DNA of 100 μ l is added, is placed on -20 DEG C with agarose gel electrophoresis detection and saves backup.
Three, PCR amplification
With PCR primer SUT3-5 ' utrF and primer SUT3-5 ' utrR to the DNA sample of 11 rice material (table 1) blades
PCR amplification is carried out, reaction system total volume is 15 μ L, wherein 7.5 μ L (TaKaRa) of Taq PCR Master Mix,
1.5 μ L, 10pmol/L primer SUT3-5 ' utrR of 10pmol/L primer SUT3-5 ' utrF, 1.5 μ L, template DNA 2 μ L, it is sterile
2.5 μ L of distilled water；After completing PCR reaction according to above-mentioned preparation, gently concussion is mixed, and PCR pipe is put into Eppendorf PCR instrument
In, setting response procedures are reacted as follows: 94 DEG C of initial denaturation 4min, circulation interior 94 DEG C of denaturation 40s, 60 DEG C of annealing 1min, and 72
DEG C renaturation 1min, 72 DEG C of extension 10min after 35 circulations, can detect 96 samples, the primer SUT3-5 ' utrF simultaneously every time
Base sequence as shown in SEQ ID NO:1, the base sequence of primer SUT3-5 ' utrR is as shown in SEQ ID NO:2.
Four, electrophoresis detection
To it is above-mentioned using 11 rice varieties leaf DNAs the PCR of template after the reaction was completed, respectively take the PCR amplification of 5 μ L to produce
Object, using 2.5% Ago-Gel, ethidium bromide (EB) is dyed after voltage 60V electrophoresis 2 hours, in gel imaging system
Under take pictures.
For trying electrophoresis result such as Fig. 2 of the PCR product of rice material.As a result as it can be seen that the kind of swimming lane 1-3,5-7,9-11
Amplifier molecule amount it is larger, be 743bp；And the amplified band molecular weight of swimming lane 4 and 8 is smaller, is 712bp.In conjunction with Fig. 1, swimming lane
The experimental cultivar of 1-3,5-7 and 9-11 are the rice germplasm of OsSUT3 gene a large amount expression, and two kinds of swimming lane 4 and 8 are
The rice germplasm of OsSUT3 gene low amounts expression.
Sequence table
<110>Yunnan Prov Agriculture University
<120>a kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 19
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 1
cacaatgtct caccgctcc 19
<210> 2
<211> 21
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
caggcagcat aagaggaaat c 21
Claims (2)
1. a kind of specific primer SUT3-5 ' utr of screening OsSUT3 gene a large amount expression rice germplasm, which is characterized in that institute
It states specific primer SUT3-5 ' utr to be made of primer SUT3-5 ' utrF and primer SUT3-5 ' utrR, primer SUT3-5 ' utrF
Base sequence as shown in SEQ ID NO:1, the base sequence of primer SUT3-5 ' utrR is as shown in SEQ ID NO:2.
2. a kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening, comprising the following steps:
(1) total DNA of rice germplasm to be measured is extracted；
(2) amplification of DNA target fragment is carried out in PCR instrument with specific primer SUT3-5 ' utr described in claim 1；
(3) after the completion of PCR amplification, 5 μ L amplified productions are taken, using 2.5% Ago-Gel, after voltage 60V electrophoresis 2 hours
Ethidium bromide staining is taken pictures under gel imaging system；
(4) pcr amplification product of rice germplasm to be measured is 743bp band, then shows that the rice germplasm is OsSUT3 gene a large amount
The rice germplasm of expression；The pcr amplification product of rice germplasm to be measured is 712bp band, then shows that the rice germplasm is OsSUT3
The rice germplasm of gene low amounts expression.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811592821.3A CN109385489B (en) | 2018-12-25 | 2018-12-25 | PCR method for screening OsSUT3 gene high-expression rice germplasm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811592821.3A CN109385489B (en) | 2018-12-25 | 2018-12-25 | PCR method for screening OsSUT3 gene high-expression rice germplasm |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109385489A true CN109385489A (en) | 2019-02-26 |
| CN109385489B CN109385489B (en) | 2019-12-20 |
Family
ID=65430912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811592821.3A Active CN109385489B (en) | 2018-12-25 | 2018-12-25 | PCR method for screening OsSUT3 gene high-expression rice germplasm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109385489B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114085847A (en) * | 2021-12-22 | 2022-02-25 | 云南农业大学 | Rice OsSUT3 gene mutant and molecular identification method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1492925A (en) * | 2001-01-05 | 2004-04-28 | �ձ��̲ݲ�ҵ��ʽ���� | Rice sucrose transporter gene promoter |
| CN101851629A (en) * | 2010-05-24 | 2010-10-06 | 中国科学院遗传与发育生物学研究所 | Method for improving crop output by converting sucrose transport protein gene OsSUT5Z of rice |
| CN105132412A (en) * | 2015-07-29 | 2015-12-09 | 福建省农业科学院生物技术研究所 | Arf1 gene 3'-UTR (untranslated region) and application of arf1 gene 3'-UTR in gene expression control |
-
2018
- 2018-12-25 CN CN201811592821.3A patent/CN109385489B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1492925A (en) * | 2001-01-05 | 2004-04-28 | �ձ��̲ݲ�ҵ��ʽ���� | Rice sucrose transporter gene promoter |
| CN101851629A (en) * | 2010-05-24 | 2010-10-06 | 中国科学院遗传与发育生物学研究所 | Method for improving crop output by converting sucrose transport protein gene OsSUT5Z of rice |
| CN105132412A (en) * | 2015-07-29 | 2015-12-09 | 福建省农业科学院生物技术研究所 | Arf1 gene 3'-UTR (untranslated region) and application of arf1 gene 3'-UTR in gene expression control |
Non-Patent Citations (5)
| Title |
|---|
| TATSURO HIROSE ET AL.: "Disruption of a gene for rice sucrose transporter, OsSUT1,impairs pollen function but pollen maturation is unaffected", 《JOURNAL OF EXPERIMENTAL BOTANY》 * |
| WEI SIAO ET AL.: "Characterization of OsSUT2 Expression and Regulation in Germinating Embryos of Rice Seeds", 《RICE》 * |
| 孙学武 等: "水稻蔗糖转运蛋白研究进展", 《生命科学研究》 * |
| 李丹丹 等: "拟南芥、水稻SUTs基因及其上游序列的系统发生关系", 《云南农业大学学报（自然科学）》 * |
| 李丹丹 等: "水稻OsSUT3基因启动子的克隆及表达分析", 《分子植物育种》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114085847A (en) * | 2021-12-22 | 2022-02-25 | 云南农业大学 | Rice OsSUT3 gene mutant and molecular identification method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109385489B (en) | 2019-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103497954B (en) | A kind of regulate and control the protein of low temperature lower blade color and gene thereof and application | |
| Musse et al. | Structural changes in senescing oilseed rape leaves at tissue and subcellular levels monitored by nuclear magnetic resonance relaxometry through water status | |
| CN102174527B (en) | Application of upright dense cluster genes in improvement of utilization efficiency of nitrogen fertilizer | |
| CN101586102B (en) | Genomic DNA extraction method of peanut leaf blades | |
| CN115927720B (en) | Soy saponin related KASP (KASP) mark and application thereof | |
| Liu et al. | The potential role of auxin and abscisic acid balance and FtARF2 in the final size determination of Tartary buckwheat fruit | |
| Wang et al. | The novel ethylene-responsive factor CsERF025 affects the development of fruit bending in cucumber | |
| Zhao et al. | Phenotypic analysis and molecular markers of leaf senescence | |
| CN110036858B (en) | Method for establishing soybean seedling emergence period salt tolerance identification method and screening salt tolerance germplasm | |
| CN105483136B (en) | A kind of wheat anti growing out gene TaZFP18 and its application | |
| CN103421807B (en) | Application of OsMYB91 transcription factor in rice growth and stress-tolerance | |
| CN113684225B (en) | Application of tomato SlHMGA3 gene in cultivation of tomatoes with delayed fruit ripening | |
| CN109385489A (en) | A kind of PCR method of OsSUT3 gene a large amount expression rice germplasm screening | |
| Li et al. | Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity | |
| CN107868840B (en) | SSR molecular marker associated with full growth number of days and application in a kind of flax | |
| Zhang et al. | Identification and fine mapping of molecular markers closely linked to fruit spines size ss gene in cucumber (Cucumis sativus L.) | |
| CN106399491B (en) | A method of for analyzing soybean germplasm genetic integrity | |
| Zhang et al. | Grafting-induced transcriptome changes and long-distance mRNA movement in the potato/Datura stramonium heterograft system | |
| Hu et al. | CsNPF7. 2 has a potential to regulate cucumber seedling growth in early nitrogen deficiency stress | |
| CN108004332A (en) | A kind of molecular labeling for influencing the main hoof growth of pig and its application | |
| CN111187777A (en) | Application of soybean GmTST2.1 gene in soybean breeding | |
| LU500606B1 (en) | Molecular Marker Indel6 for Oil-related Gene and Application Thereof | |
| CN104774853A (en) | Rice root regulation function and application of E3 ubiquitin ligase gene OsPIW | |
| CN105861637B (en) | For quickly detecting the kit of edible sunflower cenospecies SH338 authenticity | |
| CN105861640B (en) | A kind of method of Rapid identification edible sunflower cenospecies SH338 authenticity and purity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |