CN109384858A - A kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide - Google Patents
A kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide Download PDFInfo
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- CN109384858A CN109384858A CN201811520656.0A CN201811520656A CN109384858A CN 109384858 A CN109384858 A CN 109384858A CN 201811520656 A CN201811520656 A CN 201811520656A CN 109384858 A CN109384858 A CN 109384858A
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- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 136
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- -1 selenium polysaccharide Chemical class 0.000 title claims abstract description 59
- 238000000605 extraction Methods 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
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- 238000007605 air drying Methods 0.000 claims description 14
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- 239000000243 solution Substances 0.000 claims description 9
- 238000002137 ultrasound extraction Methods 0.000 claims description 8
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- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
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- 239000002245 particle Substances 0.000 description 1
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- 235000015067 sauces Nutrition 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Inorganic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of extraction preparation methods of selenium-rich jerusalem artichoke selenium polysaccharide, are related to selenium-rich product scope.The method of the extraction separation and purification of selenium synanthrin is equal to synanthrin.Impure selenium polysaccharide is obtained using water extraction, suction filtration concentration, ethanol precipitation, after de- albumen processing, recycling centrifugal drying obtains dry powder selenium polysaccharide.Jerusalem artichoke dry powder selenium polysaccharide after processing is dry being capable of long-term preservation.The optimal conditions temperature 60 that selenium polysaccharide extracts is determined, time 60min, material-water ratio 1:25 are 74.87% by the recovery rate for having done selenium polysaccharide sugar obtained by verification test under optimal conditions.Advantage is: compared with common polysaccharide, research shows that selenium polysaccharide has higher oxidation resistance, enhances the physiological activity such as immune, antitumor.
Description
Technical field
The present invention relates to selenium-rich product scope, specially a kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide.
Background technique
Immunity of organisms can be improved as microelement necessary to human life in selenium, symbol of element Se, the life to the mankind
It educates, grow and anti-aging has important health-care effect；The growth and reproductive performance of animal are improved, meat etc. is improved.Selenium is same
It is also that higher plant grows required nutrient, and has anti-oxidant, antagonism heavy metal to plant, resists adverse circumstance, enhances plant
Resistance, the metabolic effect for participating in plant, and in certain concentration range, the growth and development of plant can be promoted.
Selenium can be divided into organic selenium and inorganic selenium, and inorganic selenium mainly based on selenate, selenite, accounts for the 8% of selenium total amount,
Generally there is stronger toxicity.Organic selenium can be divided into macromolecular selenium and small molecule selenides, account for 80% or more of selenium total amount, generally
Exist in the form of seleno-amino acids and selenoprotein, the mainly organic selenides to play a role in vivo.Inorganic selenium is inhaled
Fruit of producing effects is poor, and toxicity is big；In contrast, organic selenium toxicity is low, Small side effects, the margin of safety are high, can preferably play selenium
Physiological activity.The plantation of plant selenium-rich obtains selenium polysaccharide and one of just has become a hot topic of research.In plant, selenium polysaccharide is biology
One of the principal mode that body especially plant organises to inorganic selenium, organic selenium are easier to absorb for body and utilize, and
Safety is higher.Plant selenium-rich polysaccharide belongs to organic selenium class, the dual biological activity with selenium and polysaccharide, compared with inorganic selenium
Compared with toxicity is smaller, the effect of selenium can preferably be brought into play.Selenium-rich product is increasing in recent years, especially selenium-rich agriculture
The research that product and its selenium polysaccharide extract is more.
Jerusalem artichoke belongs to the annual perennial root herbaceous plant of asteraceae helianthus, also known as Jerusalem artichoke, Jerusalem artichoke.Jerusalem artichoke not only has
Strong environmental adaptability plants the Ecological Characteristics such as extensive, and wherein (also known as inulin is poly fruit to synanthrin rich in
Sugar), account for 80% or so of 15%~20% dry weight of weight in wet base.Girasole synanthrin is a kind of biological polyoses, it passes through β by fructose molecule
(1 → 2) it is keyed, for extent of polymerization from 2~60, average out to 10, terminal is glucose unit.Synanthrin and oligofructose have double
The discrimination factor enhances effect, and synanthrin, which has, to be promoted mineral absorption, pre- preventing bone rarefaction, adjusts blood pressure and blood lipoid, adjust immune, anticancer
Etc. physiological functions, therefore synanthrin becomes one of the functional food of people's research.Currently, domestic selenium-rich product is more, foodstuff
Selenium-supply is based on health food, such as mushroom selenium, malt selenium, and in addition there are also various selenium-rich ordinary foods, such as selenium-rich rice, selenium-rich
Tea, sauce with abundant selenium, mineral water rich in selenium etc..
Currently, China supplements organic selenium by diet for the situation of human body selenium deficiency with the presence of seventy percent area selenium deficiency situation
Then become a kind of feasible method to be not susceptible to be poisoned with high security, be easily absorbed by the body utilization, and selenium-supply is high-efficient
Feature.Through research detection discovery, contain selenium more abundant in selenium-rich jerusalem artichoke, but is needed edible big by jerusalem artichoke selenium-supply merely
Measure fig, it is clear that the demand of human body selenium-supply can not be adapted to.And extract the selenium in jerusalem artichoke, then become a kind of practical
Selenium-supply mode.For this purpose, the present invention provides a kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide.
Summary of the invention
The technical problems to be solved by the invention:
For the generally existing selenium deficiency problem in China, and it is huge by the dosage that food selenium-supply needs, it is difficult to meet human body benefit
The problem of selenium demand, provides a kind of extraction preparation method of selenium-rich Girasole synanthrin.The present invention provides one using selenium-rich jerusalem artichoke as raw material
The preparation method of kind completely new selenium-rich extract, by preparing jerusalem artichoke inulin, and carried out water mention, the acquisitions such as ethanol precipitation it is thick
Polysaccharide prepares organic selenium content up to 13% or more selenium polysaccharide extract, and easy to operate, process flow is few, and extracting cycle is short,
Extraction effect is good, effectively solves the problems of prior art.
In order to solve the above technical problems, the present invention provides technical solution below:
A kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide, comprising the following steps:
1) selenium-rich jerusalem artichoke is cleaned, is cut into 3~5mm thin slice, heated-air drying will be carried out to constant weight, jerusalem artichoke thin slice is obtained, use
Superfine communication technique prepares full powder, sieves with 100 mesh sieve,；
2) part steps 1 are taken) preparation jerusalem artichoke whole powder, put it into conical flask, thereto be added jerusalem artichoke whole powder 50~
The distilled water of 85wt%, stirs evenly, and jerusalem artichoke whole powder aqueous solution is down to -40 DEG C by the rate of temperature fall of 0.5 DEG C/min, then will freeze
The jerusalem artichoke whole powder aqueous solution of knot thaws rapidly in 100 DEG C of water-baths, forms the first mixture；
3) 1~5wt% cellulase of the first mixture, 1~3wt% bromelain and 0.5~1% pectin is added
Enzyme carries out enzymatic treatment, and the temperature of enzymatic treatment is 45~55 DEG C, 60~120min of time, pH4.0~7.0；
4) the first mixture after enzymatic treatment is subjected to ultrasonic extraction, ultrasonic temperature is 65~70 DEG C, ultrasonic power 450
~550W, extraction obtain the second mixed liquor, are stripped to the second mixed liquor, and extraction temperature is 90~110 DEG C, subtract mixed liquor
Pressure is concentrated into the 1/4 of original volume, obtains third mixed liquor；
5) ethanol solution that concentration is 95% will be added in prepared third mixed liquor, carries out alcohol precipitating, third mixing
Liquid and 95% volumes of aqueous ethanol ratio are 1:10, obtain the 4th mixed liquor；
6) the 4th mixed liquor is subjected to microwave treatment, 5~10min of microwave irradiation time, 100~500W of microwave power are set
In the centrifuge of 5000r/min, it is centrifuged 15 minutes, obtains lower layer's glue selenium polysaccharide；
7) by the resulting gluey selenium polysaccharide of step 6), heated-air drying is carried out to get Thick many candies powder；
Preferably, the specific method of the heated-air drying is by selenium-rich jerusalem artichoke in 65~70 DEG C of progress hot-air seasonings, until containing
Water is lower than 18wt%.
Preferably, in the step 3), the ultrasonic extraction time is 60~65min, and extraction times are 15~20min.
Preferably, in the step 4), the alcohol sedimentation time is 12~13 hours.
Preferably, in the step 5), density of the gluey selenium polysaccharide at 60 DEG C is 1.15~1.37g/mL.
Preferably, in the step 6), the temperature of heated-air drying is 90 DEG C.
A kind of selenium-rich jerusalem artichoke selenium polysaccharide product prepared using the above method.
It is that the present invention obtains the utility model has the advantages that
Applicants have found that jerusalem artichoke selenium-rich position is at its stem tuber, Se content can reach 8.563mg/kg.Its
Extraction efficiency and the preparation of selenium polysaccharide in the application and the control of reaction condition are closely bound up.By in single factor experiment base
On plinth, using Box-Behnken experimental design and Responds Surface Methodology, ultrasonic extraction jerusalem artichoke selenium polysaccharide optimum process condition is obtained
(ultrasonic time 60min, ultrasonic power 450W, liquid-to-solid ratio 25:1), selenium polysaccharide recovery rate is up to 13.71%.By step it
Between synergistic effect so that the recovery rate of selenium polysaccharide of the present invention be higher than the prior art 5~10%, have remarkable progress.
In the present invention, cooperates between each step, is progressive, can effectively promote the yield and yield of selenium polysaccharide,
Generated general effect is significantly larger than the simple adduction of effect caused by single technological means.In the present invention, pass through science
Operation can effectively extract the effective active composition of raw material, and polysaccharide contained by jerusalem artichoke promotes inorganic selenium to be preferably changed into organic selenium,
Effective use is without the effective component in jerusalem artichoke.Meanwhile operation of the present invention is simple and easy, effect is good, high-efficient, low in cost, tool
Have significant overall economic efficiency, can batch production, large-scale production needs.
Selenium-rich Thick many candies of the invention can be used in the health care of preparation enhancing body anti-oxidation function and immunoloregulation function
In drug or food additives.Through actual verification, health care curative effect is obvious, helps to improve body immunity, anti-cancer, antitumor
Deng and product stability is good, easy to carry and take.
In extract of the invention, Se content can reach 8.563mg/kg, can be used in food processing field,
Meet the selenium-supply demand of human body, there is preferable market application prospect and social benefit.In addition, the technique side used in the present invention
Method, the active constituent that can be effectively retained in selenium-rich jerusalem artichoke have preferable effect.Simple production process of the present invention, condition temperature
With, extracting cycle is shorter, have preferable market application prospect and value.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.To facilitate a better understanding of the present invention, by following instance plus
With explanation, these examples are belonged to the scope of protection of the present invention, but do not limit the scope of the invention.
Embodiment 1
The operating procedure of the present embodiment is as follows.
1, it takes selenium-rich jerusalem artichoke to clean, is cut into 3mm thin slice, heated-air drying is carried out at a temperature of 65 DEG C to constant weight, water content 10
~18wt%.
2, the jerusalem artichoke dry powder of drying is broken into fine powder, obtains jerusalem artichoke whole powder, grinding particle size is 2~4mm of diameter.
3, the jerusalem artichoke whole powder for taking part previous step to prepare, puts it into conical flask, jerusalem artichoke whole powder is added thereto
The distilled water of 50wt%, stirs evenly, and jerusalem artichoke whole powder aqueous solution is down to -40 DEG C by the rate of temperature fall of 0.5 DEG C/min, then will freeze
The jerusalem artichoke whole powder aqueous solution of knot thaws rapidly in 100 DEG C of water-baths, forms the first mixture；
4, the 1wt% cellulase of the first mixture, 1wt% bromelain and 0.5wt% pectase is added, carries out enzyme
Processing, the temperature of enzymatic treatment are 45 DEG C, time 60min, pH4.0；
It 5, is 65 DEG C in temperature by the first mixed liquor after enzymatic treatment, power carries out ultrasonic extraction under conditions of being 500W,
65min is extracted, extraction obtains the second mixed liquor, is stripped under the conditions of at a temperature of 90 °C to the second mixed liquor, extracts
15min makes mixed liquor be concentrated under reduced pressure into the 1/4 of original volume, obtains third mixed liquor；
6, the ethanol solution that concentration is 95% will be added in prepared third mixed liquor, carries out alcohol precipitating, it is mixed obtains the 4th
Close liquid, the as concentrate of selenium polysaccharide extracting solution.Wherein, third mixed liquor and 95% volumes of aqueous ethanol ratio be 1:10, sink
The shallow lake time is 12 hours.
7, the 4th mixed liquor is subjected to microwave treatment, 5~min of microwave irradiation time, microwave power 100W are placed in 5000r/
In the centrifuge of min, it is centrifuged 15 minutes, obtains lower layer's glue selenium polysaccharide, density of the gluey selenium polysaccharide at 60 DEG C is 1.15~
1.22g/mL。
8, by resulting gluey selenium polysaccharide, 90 DEG C of heated-air dryings are carried out to get selenium-rich selenium polysaccharide dry powder.
After measured, in the present embodiment, in dry powder jerusalem artichoke selenium polysaccharide dry powder using phend-sulphuric acid measure Se content is reachable
To 8.433mg/kg.
Embodiment 2
The operating procedure of the present embodiment is as follows.
1, it takes selenium-rich jerusalem artichoke to clean, is cut into 5mm thin slice, heated-air drying is carried out at a temperature of 70 DEG C to constant weight, water content 3~
10wt%.
2, the jerusalem artichoke dry powder of drying is broken into fine powder, obtains jerusalem artichoke whole powder, sieves with 100 mesh sieve.
3, the jerusalem artichoke whole powder for taking part previous step to prepare, puts it into conical flask, jerusalem artichoke whole powder is added thereto
The distilled water of 85wt%, stirs evenly, and jerusalem artichoke whole powder aqueous solution is down to -40 DEG C by the rate of temperature fall of 0.5 DEG C/min, then will freeze
The jerusalem artichoke whole powder aqueous solution of knot thaws rapidly in 100 DEG C of water-baths, forms the first mixture；
4, the 5wt% cellulase of the first mixture, 3wt% bromelain and 1wt% pectase is added, carries out at enzyme
Reason, the temperature of enzymatic treatment are 55 DEG C, time 120min, pH7.0；
It 5, is 70 DEG C in temperature by the first mixed liquor after enzymatic treatment, power carries out ultrasonic extraction under conditions of being 450W,
60min is extracted, extraction obtains the second mixed liquor, is stripped, extracts under conditions of temperature is 110 DEG C to the second mixed liquor
20min makes mixed liquor be concentrated under reduced pressure into the 1/5 of original volume, obtains third mixed liquor；
6, the ethanol solution that concentration is 95% will be added in prepared third mixed liquor, carries out alcohol precipitating, it is mixed obtains the 4th
Close liquid, the as concentrate of selenium polysaccharide extracting solution.Wherein, third mixed liquor and 95% volumes of aqueous ethanol ratio be 1:25, sink
The shallow lake time is 13 hours.
7, the 4th mixed liquor is subjected to microwave treatment, microwave irradiation time 10min, microwave power 500W are placed in 5000r/
In the centrifuge of min, it is centrifuged 15 minutes, obtains lower layer's glue selenium polysaccharide, density of the gluey selenium polysaccharide at 60 DEG C is 1.18~
1.31g/ml。
8, by resulting gluey selenium polysaccharide, 100 DEG C of heated-air dryings are carried out to get selenium-rich selenium polysaccharide dry powder.
After measured, in the present embodiment, in dry powder jerusalem artichoke selenium polysaccharide dry powder using phend-sulphuric acid measure Se content is reachable
To 8.563mg/kg.
Embodiment 3
The operating procedure of the present embodiment is as follows:
1, it takes selenium-rich jerusalem artichoke to clean, is cut into 4mm thin slice, heated-air drying is carried out at a temperature of 60 DEG C to constant weight, water content 8~
12wt%.
2, the jerusalem artichoke dry powder of drying is broken into fine powder, obtains jerusalem artichoke whole powder, sieves with 100 mesh sieve.
3, the jerusalem artichoke whole powder for taking part previous step to prepare, puts it into conical flask, jerusalem artichoke whole powder is added thereto
The distilled water of 65wt%, stirs evenly, and jerusalem artichoke whole powder aqueous solution is down to -40 DEG C by the rate of temperature fall of 0.5 DEG C/min, then will freeze
The jerusalem artichoke whole powder aqueous solution of knot thaws rapidly in 100 DEG C of water-baths, forms the first mixture；
4, the 3wt% cellulase of the first mixture, 2wt% bromelain and 0.6wt% pectase is added, carries out enzyme
Processing, the temperature of enzymatic treatment are 50 DEG C, time 90min, pH5.5；
It 5, is 68 DEG C in temperature by the first mixed liquor after enzymatic treatment, power carries out ultrasonic extraction under conditions of being 550W,
60min is extracted, extraction obtains the second mixed liquor, is stripped, extracts under conditions of temperature is 100 DEG C to the second mixed liquor
18min makes mixed liquor be concentrated under reduced pressure into the 1/6 of original volume, obtains third mixed liquor；
6, the ethanol solution that concentration is 95% will be added in prepared third mixed liquor, carries out alcohol precipitating, it is mixed obtains the 4th
Close liquid, the as concentrate of selenium polysaccharide extracting solution.Wherein, third mixed liquor and 95% volumes of aqueous ethanol ratio be 1:15, sink
The shallow lake time is 12.5 hours.
7, the 4th mixed liquor is subjected to microwave treatment, microwave irradiation time 7min, microwave power 300W are placed in 6000r/
In the centrifuge of min, it is centrifuged 15 minutes, obtains lower layer's glue selenium polysaccharide, density of the gluey selenium polysaccharide at 60 DEG C is 1.15~
1.37g/ml。
8, by resulting gluey selenium polysaccharide, 90 DEG C of heated-air dryings are carried out to get selenium-rich selenium polysaccharide dry powder.
After measured, in the present embodiment, in dry powder jerusalem artichoke selenium polysaccharide dry powder using phend-sulphuric acid measure Se content is reachable
To 8.219mg/kg.
Comparative examples:
Selenium-rich jerusalem artichoke polysaccharide is extracted using the method for preferred embodiment in Chinese patent CN102875699A, after measured, this
In embodiment, in dry powder jerusalem artichoke selenium polysaccharide dry powder use phend-sulphuric acid measure Se content for 2.132mg/kg.
The yield of selenium-rich jerusalem artichoke polysaccharide in above-described embodiment is measured, as a result as follows:
The extraction yield of jerusalem artichoke selenium polysaccharide under 1 Different Extraction Method of table
| Embodiment | Jerusalem artichoke selenium polysaccharide extracts yield (%) |
| Embodiment 1 | 11.26% |
| Embodiment 2 | 13.71% |
| Embodiment 3 | 10.89% |
| Comparative examples | 4.77% |
In conclusion the present invention, which is remarkably improved jerusalem artichoke selenium polysaccharide, extracts yield, and the Se content extracted in product is obvious
It improves.Present invention is mainly used for functionality in the health medicine or food of preparation enhancing body anti-oxidation function and immunoloregulation function
In product additive.Its health care curative effect is obvious, compared with common polysaccharide, research shows that selenium polysaccharide have higher oxidation resistance,
Enhance the physiological activity such as immune, antitumor, while facilitating reduction human body and suffering from diabetes, cardiovascular and cerebrovascular disease, Keshan disease, big bone
Save the risk of disease, the diseases such as arthritis, and product stability is good, it is easy to carry with take.
Health-care effect mechanism after the present invention is edible is as follows:
1) selenium can enhance the anti-oxidation function of human body: selenium is the active constituent for constituting glutathione peroxidase, paddy Guang
Sweet peptide has antioxidation, can remove free radical；
2) selenium can improve immunity vascularization: a set of vascular system that tumour needs to rely on itself foundation is obtained from human body
Nutrient, to meet the needs of its fast-growth, selenium can improve the formation of immunity blood vessel, to control propagation of cancer cells and turn
It moves；
3) selenium element can selectively improve the synthesis of immunity cellular proliferation protein and answering for DNA (DNA)
System, to inhibit the growth of cancer cell, breeding.
4) selenium can activate suppressor gene p53, can induce apoptosis of tumor cells, so that cancer cell be inhibited to be mutated, inhibit cancer thin
Intracellular growth and killing cancer cell, improve cure rate；
Present invention is mainly used for functionality in the health medicine of preparation enhancing body anti-oxidation function and immunoloregulation function
Or in food additives.Its health care curative effect is obvious, compared with common polysaccharide, research shows that selenium polysaccharide has higher anti-oxidant energy
Power, the enhancing physiological activity such as immune, antitumor, at the same facilitate reduction human body suffer from diabetes, cardiovascular and cerebrovascular disease, Keshan disease,
The risk of the diseases such as Kaschin-Beck disease, arthritis, and product stability is good, it is easy to carry and take.
The above examples only illustrate the technical idea of the present invention, and this does not limit the scope of protection of the present invention, all
According to the technical idea provided by the invention, any changes made on the basis of the technical scheme each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by the prior art.
Claims (7)
1. a kind of extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide, which comprises the following steps:
1) selenium-rich jerusalem artichoke is cleaned, is cut into 3~5mm thin slice, heated-air drying will be carried out to constant weight, jerusalem artichoke thin slice is obtained, using ultra micro
Crushing technology prepares full powder, sieves with 100 mesh sieve,；
2) part steps 1 are taken) preparation jerusalem artichoke whole powder, put it into conical flask, thereto be added jerusalem artichoke whole powder 50~
The distilled water of 85wt%, stirs evenly, and jerusalem artichoke whole powder aqueous solution is down to -40 DEG C by the rate of temperature fall of 0.5 DEG C/min, then will freeze
The jerusalem artichoke whole powder aqueous solution of knot thaws rapidly in 100 DEG C of water-baths, forms the first mixture；
3) 1~5wt% cellulase of the first mixture, 1~3wt% bromelain and 0.5~1% pectase is added, into
Row enzymatic treatment, the temperature of enzymatic treatment are 45~55 DEG C, 60~120min of time, pH4.0~7.0；
4) by after enzymatic treatment the first mixture carry out ultrasonic extraction, ultrasonic temperature be 65~70 DEG C, ultrasonic power be 450~
550W, extraction obtain the second mixed liquor, are stripped to the second mixed liquor, and extraction temperature is 90~110 DEG C, depressurize mixed liquor
It is concentrated into the 1/4 of original volume, obtains third mixed liquor；
5) will in prepared third mixed liquor be added concentration be 95% ethanol solution, carry out alcohol precipitating, third mixed liquor with
95% volumes of aqueous ethanol ratio is 1:10, obtains the 4th mixed liquor；
6) the 4th mixed liquor is subjected to microwave treatment, 5~10min of microwave irradiation time, 100~500W of microwave power are placed in
In the centrifuge of 5000r/min, it is centrifuged 15 minutes, obtains lower layer's glue selenium polysaccharide；
7) by the resulting gluey selenium polysaccharide of step 6), heated-air drying is carried out to get Thick many candies powder.
2. the extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide according to claim 1, which is characterized in that described in step 1)
The specific method of heated-air drying is by selenium-rich jerusalem artichoke in 65~70 DEG C of progress hot-air seasonings, until water content is lower than 18wt%.
3. the extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide according to claim 1, which is characterized in that in the step 3),
The ultrasonic extraction time is 60~65min, and extraction times are 15~20min.
4. the extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide according to claim 1, which is characterized in that in the step 4),
The alcohol sedimentation time is 12~13 hours.
5. the extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide according to claim 1, which is characterized in that in the step 5),
Density of the gluey selenium polysaccharide at 60 DEG C is 1.15~1.37g/mL.
6. the extraction preparation method of selenium-rich jerusalem artichoke selenium polysaccharide according to claim 1, which is characterized in that in the step 6),
The temperature of heated-air drying is 90 DEG C.
7. using the product of any one of claim 1-6 the method preparation.
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