CN109381486A - A kind of technique preparing transfer factor - Google Patents
A kind of technique preparing transfer factor Download PDFInfo
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- CN109381486A CN109381486A CN201710670964.0A CN201710670964A CN109381486A CN 109381486 A CN109381486 A CN 109381486A CN 201710670964 A CN201710670964 A CN 201710670964A CN 109381486 A CN109381486 A CN 109381486A
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- transfer factor
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- homogenate
- spleen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/26—Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Developmental Biology & Embryology (AREA)
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- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention discloses a kind of technique for preparing transfer factor, the specific steps are that: spleen is cleaned, rubbing, protein isolate matter molecule, homogenate pH adjusting, cohesion, separates supernatant and filtering.Although this technique greatlies simplify the separation and Extraction process of transfer factor at present there are many method of separation and Extraction transfer factor, the production cycle is shortened, cost has been saved, is easy to operate, being suitble to industrialized production.
Description
Technical field
The present invention relates to pharmaceutical technology fields, in particular to a kind of technique for preparing transfer factor.
Background technique
Transfer factor be found in the 1950s, and be applied to clinic the 1970s, be it is a kind of can transmitting it is slow
Hair property the biologically active polypeptide and nucleotide of hypersensitivity, molecular weight less than 10000 mixture, it is active not
Destroyed by trypsase, ribalgilase, it is heat-resisting, without kind boundary the characteristics of.
The main component of transfer factor is polypeptide, amino acid and the polynucleotides etc. extracted in health pig or cattle spleen.Face
Bed can be used for assisting in the treatment of certain antibiotic and be difficult to control viral or mycotic intracellular infection (as band closes bleb, popularity
Encephalitis B, Candida albicans infection, vital myocarditis etc.), auxiliary therapeutical agent can be used as to malignant tumour, to immune deficiency
Disease, such as eczema, decrease of platelet, multiple Infectious syndrome and chronic skin mucous membrane nosomycosis, there is certain curative effect.
Currently, the preparation process of transfer factor usually according to the following steps successively implement: spleen cleaning, chopping, colloid mill,
Freeze thawing, centrifugation, supernatant liquid filtering, ultrafiltration, finally obtain transfer factor.In above-mentioned preparation process, it is centrifuged in transfer factor system
A very important processing step in standby, temperature of charge is no more than 8 degrees Celsius when centrifugation, it is necessary to using cryogenic freezing from
Scheming, however, being suitble to industrial cryogenic freezing centrifuge expensive, investment is big, and takes time and effort, and influences yield and effect
Rate.
Summary of the invention
The object of the present invention is to provide a kind of techniques for preparing transfer factor, in conjunction with physics and chemical technology, by freeze thawing
Afterwards, centrifugation step is omitted, separation of solid and liquid can be directly realized by, be obtained by filtration after supernatant and obtain transfer factor through ultrafiltration.The present invention
Using following technical scheme:
A kind of technique preparing transfer factor comprising following steps:
(1) take fresh or freezing spleen and lymph node 6-10 DEG C at a temperature of remove fat and connective tissue, and with pure
Change water to clean；
(2) tissue cleaned in step (1) is cut into small pieces, is rubbed with meat grinder, and injection is added in the ratio of 1:1
Water；
(3) pH value is adjusted to 8-8.5 with sodium hydroxide solution, and 5/1000ths formalin is added；
(4) tissue mashing machine's smudge cells are used, homogenate is made, sets -20 DEG C of multigelations 2 times, melt temperature is no more than 37
℃；
(5) homogenate is poured out to container, is adjusted with acid pH value to 3.5-4.2, and flocculant is added；
(6) that -20 DEG C of multigelations are set in the homogenate that step (5) obtains is multiple, and melt temperature is no more than 37 DEG C, until spleen
It is organized into spongy, takes supernatant；
(7) supernatant that step (6) obtains is filtered with miillpore filter, then carries out ultrafiltration with ultrafiltration membrane.
Preferably, the spleen in step (1) and lymph node 8 DEG C at a temperature of remove fat and connective tissue.
Preferably, pH value is adjusted to 8.3 in step (3).
Preferably, pH value is adjusted to 3.8 using hydrochloric acid or glacial acetic acid in step (5).
Preferably, the flocculant in step (5) is chitosan.
Preferably, the aperture of the miillpore filter in step (7) is 0.45 μm, and the molecular cut off of ultrafiltration membrane is 5000 dongles
?.
Homologous protein has like charges in aqueous solution, mutually exclusive, and protein surface can form hydration shell, this
It is sufficiently stable to allow for protein solution (actually colloid).For this purpose, the pH of protein is adjusted to alkali by one aspect of the present invention
Property, protein molecule at this moment is in isoelectric state, keeps molecule unstable；On the other hand, the protection work that formaldehyde eliminates moisture film is added
With, make protein precipitation, and increase the hardness of sediment, improve separating effect.
The process of the technique of preparation transfer factor of the invention are as follows: spleen is cleaned, is rubbing, protein isolate matter molecule, even
PH is starched to adjust, cohesion, separate supernatant and filtering.It joined formaldehyde in the technique of preparation transfer factor of the invention, increase
The hardness of sediment, improves separating effect, in addition, joined flocculant, enhances separating effect using flocculant, makes albumen
The cohesion of matter molecule.Although at present there are many method of separation and Extraction transfer factor, this technique greatlies simplify transfer factor
The centrifugation work in traditional transfer factor preparation process is omitted in separation and Extraction process, the technique of preparation transfer factor of the invention
Skill shortens the production cycle, has saved cost, is easy to operate, being suitble to industrialized production, have good to omit centrifuge
Application prospect.
Specific embodiment
Below with reference to the embodiment of the present invention, technical solution of the present invention is clearly and completely described, it is clear that retouched
The embodiment stated is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiment of the present invention, ability
Domain those of ordinary skill every other embodiment obtained without making creative work, belongs to of the invention
Protection scope.It should be noted that part referred to herein each means parts by weight.
Embodiment
(1) fresh or freezing spleen and lymph node is taken down fat and connective tissue in 8 DEG C of low temperature environment, and with pure
Change water to clean, weighing；
(2) tissue cleaned in step (1) is cut into small pieces, is rubbed with meat grinder, meat gruel shape is become, according to meat gruel
Water for injection is added in the ratio that volume ratio with water for injection is 1:1；
(3) sodium hydroxide solution is added, pH value is adjusted to 8.3, and 5/1000ths formalin is added, with this side
Formula precipitates protein molecule, improves separating effect；
(4) tissue mashing machine's smudge cells are used, homogenate is made, manufactured homogenate is placed in -20 DEG C of multigelations 2 times, is melted
Change temperature and is no more than 37 DEG C；
(5) homogenate in step (4) after multigelation is poured out to container, is adjusted to 3.8 for pH value is homogenized with acetic acid,
And chitosan is added；
(6) homogenate that step (5) obtains is set -20 DEG C of multigelations 3 times, melt temperature is no more than 37 DEG C, until spleen
It is organized into spongy, supernatant can be isolated from spongy tissue by way of extruding；
(7) supernatant that step (6) obtains is filtered with 0.45 μm of miillpore filter, is then with molecular cut off
The ultrafiltration membrane of 5000 dalton carries out ultrafiltration, obtains transfer factor.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Subject to enclosing.
Claims (6)
1. a kind of technique for preparing transfer factor, which comprises the following steps:
(1) take fresh or freezing spleen and lymph node 6-10 DEG C at a temperature of remove fat and connective tissue, and use purified water
It cleans；
(2) tissue cleaned in step (1) is cut into small pieces, is rubbed with meat grinder, and water for injection is added in the ratio of 1:1；
(3) pH value is adjusted to 8-8.5 with sodium hydroxide solution, and 5/1000ths formalin is added；
(4) tissue mashing machine's smudge cells are used, homogenate is made, sets -20 DEG C of multigelations 2 times, melt temperature is no more than 37 DEG C；
(5) homogenate is poured out to container, is adjusted with acid pH value to 3.5-4.2, and flocculant is added；
(6) that -20 DEG C of multigelations are set in the homogenate that step (5) obtains is multiple, and melt temperature is no more than 37 DEG C, until spleen tissue
At spongy, supernatant is taken；
(7) supernatant that step (6) obtains is filtered with miillpore filter, then carries out ultrafiltration with ultrafiltration membrane.
2. technique according to claim 1, which is characterized in that spleen and lymph node in step (1) 8 DEG C at a temperature of
Remove fat and connective tissue.
3. technique according to claim 2, which is characterized in that pH value is adjusted to 8.3 in step (3).
4. technique described in any one of -3 according to claim 1, which is characterized in that use hydrochloric acid or ice in step (5)
PH value is adjusted to 3.8 by acetic acid.
5. technique according to claim 1, which is characterized in that the flocculant in step (5) is chitosan.
6. technique according to claim 1, which is characterized in that the aperture of the miillpore filter in step (7) is 0.45 μm, is surpassed
The molecular cut off of filter membrane is 5000 dalton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710670964.0A CN109381486A (en) | 2017-08-08 | 2017-08-08 | A kind of technique preparing transfer factor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710670964.0A CN109381486A (en) | 2017-08-08 | 2017-08-08 | A kind of technique preparing transfer factor |
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| Publication Number | Publication Date |
|---|---|
| CN109381486A true CN109381486A (en) | 2019-02-26 |
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ID=65414035
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710670964.0A Pending CN109381486A (en) | 2017-08-08 | 2017-08-08 | A kind of technique preparing transfer factor |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583164A (en) * | 2004-06-09 | 2005-02-23 | 北京世纪昭宝转移因子研究中心 | Preparing procedure of transfer factor for intravenous injection |
| CN101904870A (en) * | 2009-06-04 | 2010-12-08 | 天津瑞普生物技术股份有限公司 | Anti-swine flu transfer factors |
| CN102198155A (en) * | 2011-05-17 | 2011-09-28 | 青岛易邦生物工程有限公司 | Production method of pig spleen transfer factor injection |
| CN103212062A (en) * | 2013-04-18 | 2013-07-24 | 山东信得科技股份有限公司 | Preparation method of pig spleen transfer factor injection |
| CN103393721A (en) * | 2013-08-19 | 2013-11-20 | 苟鸿鹰 | Preparation technology and medical applications of compound specific transfer factor |
-
2017
- 2017-08-08 CN CN201710670964.0A patent/CN109381486A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583164A (en) * | 2004-06-09 | 2005-02-23 | 北京世纪昭宝转移因子研究中心 | Preparing procedure of transfer factor for intravenous injection |
| CN101904870A (en) * | 2009-06-04 | 2010-12-08 | 天津瑞普生物技术股份有限公司 | Anti-swine flu transfer factors |
| CN102198155A (en) * | 2011-05-17 | 2011-09-28 | 青岛易邦生物工程有限公司 | Production method of pig spleen transfer factor injection |
| CN103212062A (en) * | 2013-04-18 | 2013-07-24 | 山东信得科技股份有限公司 | Preparation method of pig spleen transfer factor injection |
| CN103393721A (en) * | 2013-08-19 | 2013-11-20 | 苟鸿鹰 | Preparation technology and medical applications of compound specific transfer factor |
Non-Patent Citations (2)
| Title |
|---|
| 刘尧等: ""脾脏转移因子提取影响因素的研究"", 《中国动物保健》 * |
| 顾平等: ""猪脾转移因子生产工艺的改进"", 《中国生化药物杂志》 * |
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Application publication date: 20190226 |