CN109370930A - A kind of Se-enriched yeast and preparation method thereof - Google Patents
A kind of Se-enriched yeast and preparation method thereof Download PDFInfo
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Abstract
To solve the deficiencies in the prior art, the present invention provides a kind of preparation methods of Se-enriched yeast, comprising: S1. carries out Spawn incubation in fastening tank, obtains preculture bacteria culture fluid.S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains Se-enriched yeast liquid.S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.The Se-enriched yeast obtained using preparation method of the present invention, Se content are 2000mg/kg or more, and selenium methionine content can increase by 20% or more compared with prior art.Scale steady production may be implemented in preparation method of the present invention, and selenium additive utilization rate is high, can obviously reduce the usage amount of selenium additive, reduces production cost.
Description
Technical field
The invention belongs to saccharomycete processing technique fields, and in particular to a kind of Se-enriched yeast and preparation method thereof.
Background technique
Selenium is a kind of necessary microelement of humans and animals, is the important component of a variety of enzymes, and selenium is glutathione mistake
Oxide enzyme important component protects biomembrane from damage, maintains the normal function of cell by redox.Selenium can be with
It plays strengthen immunity, protect the functions such as cardiovascular, promotion growth, antitumor, removing toxic substances, adjusting metabolism.In China, selenium deficiency causes
Endemic disease have Keshan disease and Kaschin-Beck disease.Se-enriched yeast is obvious for supplement some diseases effect because of caused by selenium deficiency, separately
There are many nutritional ingredients abundant in outer yeast, synergistic effect can be played.
Recent domestic is relatively more to Se-enriched yeast research, but seldom energy mass production, main issue is that (1) selenium contains
Measure it is lower, generally in 1000mg/kg hereinafter, high in 1500mg/kg.(2) inorganic Se content is low, and having part is simple ferment
The absorption of female and selenium, selenium methionine content are lower.(3) certain methods are not suitable for mass production, and repeatability is very poor, some can be produced, but
It is that efficiency is very low.(4) low to the utilization rate of selenium.
Summary of the invention
In view of the problems of the existing technology the present invention, provides a kind of preparation method of Se-enriched yeast, comprising:
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid.
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains richness
Selenium yeast liquid.
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
Further, the method that the be set forth in fastening tank of step S1 carries out Spawn incubation includes: the card that strain is connected to 8-12L
Fermented and cultured 15-24 hours in formula tank.Media environment in the fastening tank are as follows: sugar content 5-25wt.%, pH value 4-
6.5, temperature is 26-35 DEG C.
Further, the method for Liquid Culture being carried out in the be set forth in PC tank of step S2 includes: to connect preculture bacteria culture fluid
To 8-12m3PC tank in fermented and cultured 18-24 hours.Media environment in the PC tank are as follows: sugar content 5-25wt.%,
PH value is 4-6.5, and temperature is 26-35 DEG C.
Further, classification liquid seeds culture described in step S3 includes: level liquid seed culture and secondary liquid kind
Son culture.
Further, the method for the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 55-70m3
PC tank in fermented and cultured 15-24 hours.Media environment in the PC tank are as follows: sugar content 5-25wt.%, pH value 4-
6.5, temperature is 26-35 DEG C.Level liquid seed culture fluid is obtained after the completion of culture.
Further, the method for the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 250-
300m3PC tank in fermented and cultured 15-24 hours.Media environment in the PC tank are as follows: sugar content 5-25wt.%, PH
Value is 4-6.5, and temperature is 26-35 DEG C.
It further, include: that will cultivate bacteria liquid seed using the method that Se-containing nutritive liquid is cultivated described in step S4
It is connected to 340-400m3PC tank in fermented and cultured 15-24 hours, while in batches add selenium element substance.In the PC tank
Media environment are as follows: sugar content 5-25wt.%, pH value 4-6.5, temperature are 26-35 DEG C.
Further, the method for adding selenium element substance in batches includes: to add one when cultivating bacteria liquid seed
Secondary selenium element substance, additive amount are the 30-35% of total addition level.When fermented and cultured carries out 1-2h, a selenium element object is added
Matter, additive amount are the 30-35% of total addition level.When fermented and cultured is carried out to the half of preset time, a selenium element is added
Substance, additive amount are the 20-25% of total addition level.When fermented and cultured is carried out to the 3/4 of preset time, the selenium member of surplus is added
Plain substance.The total addition level of the selenium element substance is the 0.05-0.2% of culture medium quality.The selenium element substance is sub- selenium
Sour sodium.
Further, dry dry using 60-140 DEG C of boiling type described in step S5.
Further, the sugar is that one or both of brewer's wort or molasses compound.
Further, when carrying out fermented and cultured using the culture medium in the PC tank, nitrogen source and phosphorus source are required supplementation with, it is used
Nutrient source using fed-batch mode add.The nitrogen source are as follows: one of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.
Phosphorus source is ammonium dihydrogen phosphate.The additive amount of the nitrogen source and phosphorus source is the 5-30% of the culture medium quality in PC tank.
In addition, the Se-enriched yeast uses the preparation method of selenium-rich Saccharomyces cerevisiae the present invention also provides a kind of Se-enriched yeast
It is prepared.
One of the present invention has at least the following advantages:
1. the Se-enriched yeast obtained using preparation method of the present invention, Se content is 2000mg/kg or more.
2. the Se-enriched yeast obtained using preparation method of the present invention, selenium methionine content can increase by 20% compared with prior art
More than.
3. scale steady production may be implemented in preparation method of the present invention, and selenium additive utilization rate is high, can obviously reduce
The usage amount of selenium additive reduces production cost.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, below to of the invention further detailed
Explanation.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not intended to limit the present invention.
Embodiment 1
A kind of Se-enriched yeast is prepared using following preparation method:
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid.
The method that the be set forth in fastening tank of step S1 carries out Spawn incubation includes: that strain is connected in the fastening tank of 10L to ferment
Culture 20 hours.Media environment in the fastening tank are as follows: sugar content 5-25wt.%, pH value 6, temperature are 30 DEG C.
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.
It includes: that preculture bacteria culture fluid is connected to 10m that the method for Liquid Culture is carried out in the be set forth in PC tank of step S23PC
Fermented and cultured 20 hours in tank.Media environment in the PC tank are as follows: sugar content 20wt.%, pH value 6, temperature 30
℃。
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.
Classification liquid seeds culture includes: level liquid seed culture and secondary liquid seed culture described in step S3.
The method of the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 60m3PC tank in ferment
Culture 20 hours.Media environment in the PC tank are as follows: sugar content 20wt.%, pH value 6, temperature are 30 DEG C.It has cultivated
Level liquid seed culture fluid is obtained after.
The method of the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 280m3PC tank in
Fermented and cultured 20 hours.Media environment in the PC tank are as follows: sugar content 20wt.%, pH value 6, temperature are 30 DEG C.
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains richness
Selenium yeast liquid.
The method for using Se-containing nutritive liquid to be cultivated described in step S4 includes: that culture bacteria liquid seed is connected to 370m3
PC tank in fermented and cultured 20 hours, while in batches add selenium element substance.Media environment in the PC tank are as follows: containing sugar
Amount is 20wt.%, and pH value 6, temperature is 30 DEG C.
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
It is dry dry using 110 DEG C of boiling types described in step S5.
Embodiment 2
A kind of Se-enriched yeast is prepared using following preparation method:
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid.
The method that the be set forth in fastening tank of step S1 carries out Spawn incubation includes: that strain is connected in the fastening tank of 12L to ferment
Culture 24 hours.Media environment in the fastening tank are as follows: sugar content 25wt.%, pH value 6.5, temperature are 35 DEG C.
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.
It includes: that preculture bacteria culture fluid is connected to 12m that the method for Liquid Culture is carried out in the be set forth in PC tank of step S23PC
Fermented and cultured 24 hours in tank.Media environment in the PC tank are as follows: sugar content 25wt.%, pH value 6.5, temperature are
35℃。
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.
Classification liquid seeds culture includes: level liquid seed culture and secondary liquid seed culture described in step S3.
The method of the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 70m3PC tank in ferment
Culture 24 hours.Media environment in the PC tank are as follows: sugar content 25wt.%, pH value 6.5, temperature are 35 DEG C.Culture
Level liquid seed culture fluid is obtained after the completion.
The method of the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 300m3PC tank in
Fermented and cultured 24 hours.Media environment in the PC tank are as follows: sugar content 25wt.%, pH value 6.5, temperature are 35 DEG C.
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains richness
Selenium yeast liquid.
The method for using Se-containing nutritive liquid to be cultivated described in step S4 includes: that culture bacteria liquid seed is connected to 400m3
PC tank in fermented and cultured 24 hours, while in batches add selenium element substance.Media environment in the PC tank are as follows: containing sugar
Amount is 25wt.%, and pH value 6.5, temperature is 35 DEG C.
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
It is dry dry using 140 DEG C of boiling types described in step S5.
Embodiment 3
A kind of Se-enriched yeast is prepared using following preparation method:
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid.
The method that the be set forth in fastening tank of step S1 carries out Spawn incubation includes: training of fermenting in the fastening tank for be connected to strain 8L
It supports 15 hours.Media environment in the fastening tank are as follows: sugar content 5wt.%, pH value 4, temperature are 26 DEG C.
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.
It includes: that preculture bacteria culture fluid is connected to 8m that the method for Liquid Culture is carried out in the be set forth in PC tank of step S23PC
Fermented and cultured 18 hours in tank.Media environment in the PC tank are as follows: sugar content 5wt.%, pH value 4, temperature 26
℃。
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.
Classification liquid seeds culture includes: level liquid seed culture and secondary liquid seed culture described in step S3.
The method of the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 55m3PC tank in ferment
Culture 15 hours.Media environment in the PC tank are as follows: sugar content 5wt.%, pH value 4, temperature are 26 DEG C.It has cultivated
Level liquid seed culture fluid is obtained after.
The method of the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 250m3PC tank in
Fermented and cultured 15 hours.Media environment in the PC tank are as follows: sugar content 5wt.%, pH value 4, temperature are 26 DEG C.
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains richness
Selenium yeast liquid.
The method for using Se-containing nutritive liquid to be cultivated described in step S4 includes: that culture bacteria liquid seed is connected to 340m3
PC tank in fermented and cultured 15 hours, while in batches add selenium element substance.Media environment in the PC tank are as follows: containing sugar
Amount is 5wt.%, and pH value 4, temperature is 26 DEG C.
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
It is dry dry using 60 DEG C of boiling types described in step S5.
Embodiment 4
A kind of Se-enriched yeast is prepared using following preparation method:
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid.
The method that the be set forth in fastening tank of step S1 carries out Spawn incubation includes: that strain is connected in the fastening tank of 11L to ferment
Culture 18 hours.Media environment in the fastening tank are as follows: sugar content 15wt.%, pH value 5, temperature are 32 DEG C.
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained.
It includes: that preculture bacteria culture fluid is connected to 10m that the method for Liquid Culture is carried out in the be set forth in PC tank of step S23PC
Fermented and cultured 22 hours in tank.Media environment in the PC tank are as follows: sugar content 20wt.%, pH value 5.5, temperature are
28℃。
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed.
Classification liquid seeds culture includes: level liquid seed culture and secondary liquid seed culture described in step S3.
The method of the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 62m3PC tank in ferment
Culture 18 hours.Media environment in the PC tank are as follows: sugar content 22wt.%, pH value 6, temperature are 28 DEG C.It has cultivated
Level liquid seed culture fluid is obtained after.
The method of the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 280m3PC tank in
Fermented and cultured 18 hours.Media environment in the PC tank are as follows: sugar content 8wt.%, pH value 4.5, temperature are 33 DEG C.
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains richness
Selenium yeast liquid.
The method for using Se-containing nutritive liquid to be cultivated described in step S4 includes: that culture bacteria liquid seed is connected to 345m3
PC tank in fermented and cultured 21 hours, while in batches add selenium element substance.Media environment in the PC tank are as follows: containing sugar
Amount is 18wt.%, and pH value 5, temperature is 31 DEG C.
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
It is dry dry using 110 DEG C of boiling types described in step S5.
Embodiment 5
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, selenium element is added described in preparation method in batches
The method of substance includes: selenium element substance of addition when cultivating bacteria liquid seed, and additive amount is the 32% of total addition level.In
When fermented and cultured carries out 1.5h, a selenium element substance is added, additive amount is the 32% of total addition level.In fermented and cultured carry out to
When the half of preset time, a selenium element substance is added, additive amount is the 22% of total addition level.It carries out in fermented and cultured to pre-
If the time 3/4 when, add the selenium element substance of surplus.The total addition level of the selenium element substance is culture medium quality
0.08%.The selenium element substance is sodium selenite.
Embodiment 6
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, selenium element is added described in preparation method in batches
The method of substance includes: selenium element substance of addition when cultivating bacteria liquid seed, and additive amount is the 35% of total addition level.In
When fermented and cultured carries out 2h, a selenium element substance is added, additive amount is the 35% of total addition level.It carries out in fermented and cultured to pre-
If when the half of time, adding a selenium element substance, additive amount is the 20% of total addition level.It carries out in fermented and cultured to default
Time 3/4 when, add the selenium element substance of surplus.The total addition level of the selenium element substance is the 0.2% of culture medium quality.
The selenium element substance is sodium selenite.
Embodiment 7
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, selenium element is added described in preparation method in batches
The method of substance includes: selenium element substance of addition when cultivating bacteria liquid seed, and additive amount is the 30% of total addition level.In
When fermented and cultured carries out 1h, a selenium element substance is added, additive amount is the 30% of total addition level.It carries out in fermented and cultured to pre-
If when the half of time, adding a selenium element substance, additive amount is the 25% of total addition level.It carries out in fermented and cultured to default
Time 3/4 when, add the selenium element substance of surplus.The total addition level of the selenium element substance is culture medium quality
0.05%.The selenium element substance is sodium selenite.
Embodiment 8
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, sugar described in preparation method is brewer's wort or molasses
One or both of compounding.
Embodiment 9
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, using the culture in the PC tank in preparation method
When base carries out fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode.The nitrogen source are as follows:
One of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.Phosphorus source is ammonium dihydrogen phosphate.The nitrogen source and phosphorus source
Additive amount be 20% of the culture medium quality in PC tank.
Embodiment 10
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, using the culture in the PC tank in preparation method
When base carries out fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode.The nitrogen source are as follows:
One of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.Phosphorus source is ammonium dihydrogen phosphate.The addition of the nitrogen source
Amount is 5% of the culture medium quality in PC tank.The additive amount of phosphorus source is 30% of the culture medium quality in PC tank.
Embodiment 11
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, using the culture in the PC tank in preparation method
When base carries out fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode.The nitrogen source are as follows:
One of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.Phosphorus source is ammonium dihydrogen phosphate.The addition of the nitrogen source
Amount is 30% of the culture medium quality in PC tank.The additive amount of phosphorus source is 5% of the culture medium quality in PC tank.
Embodiment 12
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, using the culture in the PC tank in preparation method
When base carries out fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode.The nitrogen source are as follows:
One of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.Phosphorus source is ammonium dihydrogen phosphate.The addition of the nitrogen source
Amount is 15% of the culture medium quality in PC tank.The additive amount of phosphorus source is 20% of the culture medium quality in PC tank.
Embodiment 13
Based on Se-enriched yeast described in embodiment 1-4 any embodiment, using the culture in the PC tank in preparation method
When base carries out fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode.The nitrogen source are as follows:
One of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings.Phosphorus source is ammonium dihydrogen phosphate.The addition of the nitrogen source
Amount is 10% of the culture medium quality in PC tank.The additive amount of phosphorus source is 25% of the culture medium quality in PC tank.
One of the present invention has at least the following advantages:
1. the Se-enriched yeast obtained using preparation method of the present invention, Se content is 2000mg/kg or more.
2. the Se-enriched yeast obtained using preparation method of the present invention, selenium methionine content can increase by 20% compared with prior art
More than.
3. scale steady production may be implemented in preparation method of the present invention, and selenium additive utilization rate is high, can obviously reduce
The usage amount of selenium additive reduces production cost.
It should be noted that and understand, in the case where not departing from spirit and scope required by the claims in the present invention, energy
It is enough that various modifications and improvements are made to the present invention of foregoing detailed description.It is therefore desirable to protection technical solution range not by
The limitation of given any specific exemplary teachings.
Claims (10)
1. a kind of preparation method of Se-enriched yeast characterized by comprising
S1. Spawn incubation is carried out in fastening tank, obtains preculture bacteria culture fluid；
S2. by preculture bacteria culture fluid in carrying out Liquid Culture in PC tank, primary culture bacteria culture fluid is obtained；
S3. primary culture bacteria culture fluid separates concentration after carrying out classification liquid seeds culture, obtains culture bacteria liquid seed；
S4. bacteria liquid seed will be cultivated in fermentor, concentration is separated after being cultivated using Se-containing nutritive liquid, obtains selenium-rich ferment
Mother liquor；
S5. the Se-enriched yeast is obtained after drying Se-enriched yeast liquid.
2. the preparation method of Se-enriched yeast according to claim 1, which is characterized in that the be set forth in fastening tank of step S1 carries out bacterium
The method of kind culture includes: to be connected to strain in the fastening tank of 8-12L fermented and cultured 15-24 hours；Training in the fastening tank
Support basic ring border are as follows: sugar content 5-25wt.%, pH value 4-6.5, temperature are 26-35 DEG C.
3. the preparation method of Se-enriched yeast according to claim 1, which is characterized in that carry out liquid in the be set forth in PC tank of step S2
The method of body culture includes: that preculture bacteria culture fluid is connected to 8-12m3PC tank in fermented and cultured 18-24 hours；The PC tank
In media environment are as follows: sugar content 5-25wt.%, pH value 4-6.5, temperature be 26-35 DEG C.
4. the preparation method of Se-enriched yeast according to claim 1, which is characterized in that classification liquid seeds training described in step S3
Supporting includes: level liquid seed culture and secondary liquid seed culture；
The method of the level liquid seed culture includes: that primary culture bacteria culture fluid is connected to 55-70m3PC tank in ferment training
It supports 15-24 hours；Media environment in the PC tank are as follows: sugar content 5-25wt.%, pH value 4-6.5, temperature 26-
35℃；Level liquid seed culture fluid is obtained after the completion of culture；
The method of the secondary liquid seed culture includes: that level liquid seed culture fluid is connected to 250-300m3PC tank in send out
Ferment culture 15-24 hours；Media environment in the PC tank are as follows: sugar content 5-25wt.%, pH value 4-6.5, temperature are
26-35℃。
5. the preparation method of Se-enriched yeast according to claim 1, which is characterized in that use Se-containing nutritive liquid described in step S4
The method cultivated includes: that culture bacteria liquid seed is connected to 340-400m3PC tank in fermented and cultured 15-24 hours, together
When add selenium element substance in batches；Media environment in the PC tank are as follows: sugar content 5-25wt.%, pH value 4-
6.5, temperature is 26-35 DEG C.
6. the preparation method of Se-enriched yeast according to claim 5, which is characterized in that described to add selenium element substance in batches
Method include: when cultivating bacteria liquid seed addition selenium element substance, additive amount be total addition level 30-35%；In
When fermented and cultured carries out 1-2h, a selenium element substance is added, additive amount is the 30-35% of total addition level；In fermented and cultured into
Row to preset time half when, add a selenium element substance, additive amount is the 20-25% of total addition level；In fermented and cultured
Carry out to preset time 3/4 when, add the selenium element substance of surplus；The total addition level of the selenium element substance is culture substrate
The 0.05-0.2% of amount；The selenium element substance is sodium selenite.
7. the preparation method of Se-enriched yeast according to claim 1, which is characterized in that dry described in step S5 to use 60-140
DEG C boiling type is dry.
8. according to the preparation method of any Se-enriched yeast of claim 2-5, which is characterized in that the sugar is brewer's wort or sugar
One or both of honey compounding.
9. according to the preparation method of any Se-enriched yeast of claim 3-5, which is characterized in that using the training in the PC tank
When supporting base progress fermented and cultured, nitrogen source and phosphorus source are required supplementation with, nutrient source used is added using fed-batch mode；The nitrogen source
Are as follows: one of ammonium sulfate, ammonium hydroxide, ammonium dihydrogen phosphate or a variety of compoundings；Phosphorus source is ammonium dihydrogen phosphate；The nitrogen source and phosphorus
The additive amount in source is the 5-30% of the culture medium quality in PC tank.
10. a kind of Se-enriched yeast, which is characterized in that prepared using the preparation method of any Se-enriched yeast of claim 1-9
It obtains.
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| CN114276944A (en) * | 2021-12-30 | 2022-04-05 | 广东五洲药业有限公司 | Selenium-rich yeast new strain and production method of yeast selenium |
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