CN109369595A - A method of dihydromyricetin is extracted from ampelopsis grossdentata - Google Patents
A method of dihydromyricetin is extracted from ampelopsis grossdentata Download PDFInfo
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- CN109369595A CN109369595A CN201811206590.8A CN201811206590A CN109369595A CN 109369595 A CN109369595 A CN 109369595A CN 201811206590 A CN201811206590 A CN 201811206590A CN 109369595 A CN109369595 A CN 109369595A
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- ampelopsis grossdentata
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- KJXSIXMJHKAJOD-LSDHHAIUSA-N (+)-dihydromyricetin Chemical compound C1([C@@H]2[C@H](C(C3=C(O)C=C(O)C=C3O2)=O)O)=CC(O)=C(O)C(O)=C1 KJXSIXMJHKAJOD-LSDHHAIUSA-N 0.000 title claims abstract description 56
- 235000009388 Parthenocissus quinquefolia Nutrition 0.000 title claims abstract description 29
- KQILIWXGGKGKNX-UHFFFAOYSA-N dihydromyricetin Natural products OC1C(=C(Oc2cc(O)cc(O)c12)c3cc(O)c(O)c(O)c3)O KQILIWXGGKGKNX-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000219099 Parthenocissus quinquefolia Species 0.000 title claims abstract 7
- 239000000284 extract Substances 0.000 claims abstract description 7
- 239000013078 crystal Substances 0.000 claims abstract description 6
- 230000008569 process Effects 0.000 claims abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 56
- 239000000243 solution Substances 0.000 claims description 35
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 34
- 238000001914 filtration Methods 0.000 claims description 15
- 230000001376 precipitating effect Effects 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000012141 concentrate Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 230000003750 conditioning effect Effects 0.000 claims description 10
- 239000012043 crude product Substances 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
- 239000011265 semifinished product Substances 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000002137 ultrasound extraction Methods 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 241000563984 Ampelopsis Species 0.000 description 22
- 235000019441 ethanol Nutrition 0.000 description 17
- 230000000694 effects Effects 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 208000007848 Alcoholism Diseases 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 201000007930 alcohol dependence Diseases 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- -1 flavone compound Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/32—2,3-Dihydro derivatives, e.g. flavanones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of methods for extracting dihydromyricetin from ampelopsis grossdentata, comprising the following steps: (1) pre-processes；(2) it digests；(3) it extracts；(4) it is concentrated；(5) primary crystal；(6) it recrystallizes；(7) it purifies；(8) it is dried in vacuo.The present invention can improve the yield and purity of dihydromyricetin.
Description
Technical field
The present invention relates to the extracting methods of dihydromyricetin, in particular to a kind of to extract dihydromyricetin from ampelopsis grossdentata
Method.
Background technique
Ampelopsis grossdentata alias vine tea, Maoyanmei tea are a kind of wild bejucoes, and wherein main active is
Dihydromyricetin.Dihydromyricetin, which has, removes a variety of peculiar effects such as free radical, anti-oxidant, antithrombotic, antitumor, anti-inflammatory；
And dihydromyricetin is a kind of more special flavone compound, is releasing alcoholism, prevention alcoholic liver, fatty liver, inhibition
Disease incidence, the anti-hypertension, the formation for inhibiting extracorporeal platelet aggregation and internal thrombus, reduction that liver cell deteriorates, reduces liver cancer
Blood lipid and blood glucose level, improving superoxide dismutase activity and liver protecting etc. has special efficacy.And it is existing
The yield of dihydromyricetin extractive technique is not high, and the purity of resulting dihydromyricetin is also to be improved.
Summary of the invention
The purpose of the present invention is making improvement and innovation for shortcoming and defect present in background technique, provide it is a kind of from
The method that ampelopsis grossdentata extracts dihydromyricetin, this method can improve the yield and purity of dihydromyricetin.
To achieve the above object, the technical solution adopted by the present invention is that: it is a kind of to extract dihydromyricetin from ampelopsis grossdentata
Method, comprising the following steps:
(1) it pre-processes: fresh ampelopsis grossdentata leaf being toasted, then takes out and is ground into 20 mesh particles, ampelopsis grossdentata is made
Ye Fen；
(2) it digests: ampelopsis grossdentata leaf powder being mixed with deionized water, and complex enzyme is added and stirs evenly, place 1 ~ 2h, be made
Enzymolysis liquid；
(3) it extracts: enzymolysis liquid is filtered with centrifuge, obtain aqueous solution, filtered solid is put into the hot second that concentration is 90%
In alcoholic solution, 1h is handled with ultrasonic extraction tank, then filters pressing obtains ethanol solution, and aqueous solution and ethanol solution are mixed to prepare
Stoste；
(4) it is concentrated: stoste being concentrated into 0.5 times of amount of raw material weight, concentrate is made；
(5) primary crystal: adjusting the pH value of concentrate with hydrochloric acid, stand under cryogenic, precipitating is collected by filtration, and crude product is made；
(6) recrystallize: by crude product be added boiling water dissolution, and use the pH value of hydrochloric acid conditioning solution, stand under cryogenic, filtering
Precipitating is collected, is repeated twice, semi-finished product are made；
(7) it purifies: the ethanol solution that concentration is 90% is added in semi-finished product and is dissolved, and with the pH value of hydrochloric acid conditioning solution, low
It is stood under the conditions of temperature, precipitating, finished product is collected by filtration；
(8) it is dried in vacuo: placing products into dry 14 ~ 16h in vacuum oven.
Preferably, the temperature of baking described in step (1) is 90 ~ 100 DEG C, and baking time is 3 ~ 4h.
Preferably, deionized water temperature described in step (2) is 40 ~ 50 DEG C, the weight Zhan Xianchi snake Portugal of the complex enzyme
The 2% ~ 3% of grape leaf powder weight.
Preferably, salt acid for adjusting pH value is added to 4 ~ 5 in concentrate described in step (5), stands for 24 hours at 5 ~ 8 DEG C.
Preferably, salt acid for adjusting pH value is added to 4 ~ 5 in solution described in step (6) and (7), stands 12h at 5 ~ 8 DEG C.
Advantages of the present invention and the utility model has the advantages that
The present invention uses fresh ampelopsis grossdentata leaf, and by high-temperature baking, the method dried in the shade compared to tradition can be protected preferably
Dihydromyricetin is stayed, dihydromyricetin is avoided to lose, while the present invention cracks the cell wall of ampelopsis grossdentata leaf using complex enzyme,
Make intracellular substance release, improves the yield of dihydromyricetin.The present invention is assisted from enzymatic hydrolysis residue using ultrasonic wave
Dihydromyricetin is extracted, the special work such as judder, high acceleration, strong cavitation effect, stirring generated by ultrasonic wave
With, promote ethyl alcohol dissolve dihydromyricetin, to greatest extent improve dihydromyricetin yield.The present invention is adjusted molten using hydrochloric acid
The pH value of liquid greatly reduces the solubility of dihydromyricetin, while crystallizing in cryogenic conditions, improves crystalline rate to 4 ~ 5
And yield.The present invention is recrystallized to give higher degree first with deionized water using the method for recrystallization and different solvents crystallization
Dihydromyricetin, recycle 90% ethanol solution crystallizing to purify to obtain the dihydromyricetin of high-purity, purifying technique is simple,
It is low in cost, substantially increase the purity of dihydromyricetin.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1
A method of dihydromyricetin is extracted from ampelopsis grossdentata, comprising the following steps:
(1) it pre-processes: fresh ampelopsis grossdentata leaf being toasted into 3h at a temperature of 90 DEG C, then takes out and is ground into 20 mesh particles,
Ampelopsis grossdentata leaf powder is made.
(2) it digests: ampelopsis grossdentata leaf powder is mixed with 40 DEG C of deionized water, and be added and account for the compound of leaf powder weight 2%
Enzyme stirs evenly, and places 1h, and enzymolysis liquid is made.
(3) it extracts: enzymolysis liquid is filtered with centrifuge, obtain aqueous solution, it is 90% that filtered solid, which is put into concentration,
In hot ethanol solution, 1h is handled with ultrasonic extraction tank, then filters pressing obtains ethanol solution, and aqueous solution is mixed with ethanol solution
Stoste is made.
(4) it is concentrated: stoste being concentrated into 0.5 times of amount of raw material weight, concentrate is made.
(5) primary crystal: the pH value for adjusting concentrate with hydrochloric acid stands for 24 hours at 5 DEG C to 4, precipitating is collected by filtration, be made
Crude product.
(6) it recrystallizes: boiling water dissolution is added in crude product, and with the pH value of hydrochloric acid conditioning solution to 4, is stood at 5 DEG C
12h is collected by filtration precipitating, is repeated twice, and semi-finished product are made.
(7) it purifies: the ethanol solution that concentration is 90% is added in semi-finished product and is dissolved, and extremely with the pH value of hydrochloric acid conditioning solution
4,12h is stood at 5 DEG C, and precipitating, finished product is collected by filtration.
(8) it is dried in vacuo: placing products into dry 14h in vacuum oven.
Embodiment 2
A method of dihydromyricetin is extracted from ampelopsis grossdentata, comprising the following steps:
(1) it pre-processes: fresh ampelopsis grossdentata leaf being toasted into 3h at a temperature of 95 DEG C, then takes out and is ground into 20 mesh particles,
Ampelopsis grossdentata leaf powder is made.
(2) it digests: ampelopsis grossdentata leaf powder is mixed with 45 DEG C of deionized water, and be added and account for answering for leaf powder weight 2.5%
Synthase stirs evenly, and places 1.5h, and enzymolysis liquid is made.
(3) it extracts: enzymolysis liquid is filtered with centrifuge, obtain aqueous solution, it is 90% that filtered solid, which is put into concentration,
In hot ethanol solution, 1h is handled with ultrasonic extraction tank, then filters pressing obtains ethanol solution, and aqueous solution is mixed with ethanol solution
Stoste is made.
(4) it is concentrated: stoste being concentrated into 0.5 times of amount of raw material weight, concentrate is made.
(5) primary crystal: the pH value for adjusting concentrate with hydrochloric acid stands for 24 hours at 6 DEG C to 4, precipitating is collected by filtration, be made
Crude product.
(6) it recrystallizes: boiling water dissolution is added in crude product, and with the pH value of hydrochloric acid conditioning solution to 4, is stood at 6 DEG C
12h is collected by filtration precipitating, is repeated twice, and semi-finished product are made.
(7) it purifies: the ethanol solution that concentration is 90% is added in semi-finished product and is dissolved, and extremely with the pH value of hydrochloric acid conditioning solution
4,12h is stood at 6 DEG C, precipitating, finished product is collected by filtration.
(8) it is dried in vacuo: placing products into dry 15h in vacuum oven.
Embodiment 3
A method of dihydromyricetin is extracted from ampelopsis grossdentata, comprising the following steps:
(1) it pre-processes: fresh ampelopsis grossdentata leaf being toasted into 4h at a temperature of 100 DEG C, then takes out and is ground into 20 mesh particles,
Ampelopsis grossdentata leaf powder is made.
(2) it digests: ampelopsis grossdentata leaf powder is mixed with 50 DEG C of deionized water, and be added and account for the compound of leaf powder weight 3%
Enzyme stirs evenly, and places 2h, and enzymolysis liquid is made.
(3) it extracts: enzymolysis liquid is filtered with centrifuge, obtain aqueous solution, it is 90% that filtered solid, which is put into concentration,
In hot ethanol solution, 1h is handled with ultrasonic extraction tank, then filters pressing obtains ethanol solution, and aqueous solution is mixed with ethanol solution
Stoste is made.
(4) it is concentrated: stoste being concentrated into 0.5 times of amount of raw material weight, concentrate is made.
(5) primary crystal: the pH value for adjusting concentrate with hydrochloric acid stands for 24 hours at 8 DEG C to 5, precipitating is collected by filtration, be made
Crude product.
(6) it recrystallizes: boiling water dissolution is added in crude product, and with the pH value of hydrochloric acid conditioning solution to 5, is stood at 8 DEG C
12h is collected by filtration precipitating, is repeated twice, and semi-finished product are made.
(7) it purifies: the ethanol solution that concentration is 90% is added in semi-finished product and is dissolved, and extremely with the pH value of hydrochloric acid conditioning solution
5,12h is stood at 8 DEG C, precipitating, finished product is collected by filtration.
(8) it is dried in vacuo: placing products into dry 16h in vacuum oven.
The technical principle of the invention is described above in combination with a specific embodiment.These descriptions are intended merely to explain of the invention
Principle, and shall not be construed in any way as a limitation of the scope of protection of the invention.Based on the explanation herein, the technology of this field
Personnel can associate with other specific embodiments of the invention without creative labor, these modes are fallen within
Within protection scope of the present invention.
Claims (5)
1. a kind of method for extracting dihydromyricetin from ampelopsis grossdentata, it is characterised in that following steps:
(1) it pre-processes: fresh ampelopsis grossdentata leaf being toasted, then takes out and is ground into 20 mesh particles, ampelopsis grossdentata is made
Ye Fen；
(2) it digests: ampelopsis grossdentata leaf powder being mixed with deionized water, and complex enzyme is added and stirs evenly, place 1 ~ 2h, be made
Enzymolysis liquid；
(3) it extracts: enzymolysis liquid is filtered with centrifuge, obtain aqueous solution, filtered solid is put into the hot second that concentration is 90%
In alcoholic solution, 1h is handled with ultrasonic extraction tank, then filters pressing obtains ethanol solution, and aqueous solution and ethanol solution are mixed to prepare
Stoste；
(4) it is concentrated: stoste being concentrated into 0.5 times of amount of raw material weight, concentrate is made；
(5) primary crystal: adjusting the pH value of concentrate with hydrochloric acid, stand under cryogenic, precipitating is collected by filtration, and crude product is made；
(6) recrystallize: by crude product be added boiling water dissolution, and use the pH value of hydrochloric acid conditioning solution, stand under cryogenic, filtering
Precipitating is collected, is repeated twice, semi-finished product are made；
(7) it purifies: the ethanol solution that concentration is 90% is added in semi-finished product and is dissolved, and with the pH value of hydrochloric acid conditioning solution, low
It is stood under the conditions of temperature, precipitating, finished product is collected by filtration；
(8) it is dried in vacuo: placing products into dry 14 ~ 16h in vacuum oven.
2. a kind of method according to claim 1, it is characterised in that the temperature of baking described in step (1) is 90 ~ 100
DEG C, baking time is 3 ~ 4h.
3. a kind of method according to claim 1, it is characterised in that deionized water temperature described in step (2) is 40 ~ 50
DEG C, the weight of the complex enzyme accounts for the 2% ~ 3% of ampelopsis grossdentata leaf powder weight.
4. a kind of method according to claim 1, it is characterised in that salt acid for adjusting pH is added in concentrate described in step (5)
Value is stood for 24 hours at 5 ~ 8 DEG C to 4 ~ 5.
5. a kind of method according to claim 1, it is characterised in that hydrochloric acid tune is added in solution described in step (6) and (7)
PH value is saved to 4 ~ 5, stands 12h at 5 ~ 8 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811206590.8A CN109369595A (en) | 2018-10-17 | 2018-10-17 | A method of dihydromyricetin is extracted from ampelopsis grossdentata |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811206590.8A CN109369595A (en) | 2018-10-17 | 2018-10-17 | A method of dihydromyricetin is extracted from ampelopsis grossdentata |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532537A (en) * | 2022-02-23 | 2022-05-27 | 广东农工商职业技术学院 | Method for preparing dihydromyricetin nano capsule based on vine tea extract |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153091A (en) * | 2015-08-25 | 2015-12-16 | 华中科技大学同济医学院附属同济医院 | Method for increasing yield of dihydromyricetin in ampelopsis grossedentata |
| CN106518829A (en) * | 2016-11-10 | 2017-03-22 | 张清峰 | Method for separating and purifying dihydromyricetin from ampelopsis grossedentata leaves |
-
2018
- 2018-10-17 CN CN201811206590.8A patent/CN109369595A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153091A (en) * | 2015-08-25 | 2015-12-16 | 华中科技大学同济医学院附属同济医院 | Method for increasing yield of dihydromyricetin in ampelopsis grossedentata |
| CN106518829A (en) * | 2016-11-10 | 2017-03-22 | 张清峰 | Method for separating and purifying dihydromyricetin from ampelopsis grossedentata leaves |
Non-Patent Citations (2)
| Title |
|---|
| 姚茂君等: "二氢杨梅素的超声波辅助溶剂提取工艺", 《食品与生物技术学报》, vol. 26, no. 3, pages 20 - 23 * |
| 陈雁梅: "显齿蛇葡萄制茶废弃茎中二氢杨梅素制备工艺研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》, no. 02, pages 018 - 122 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532537A (en) * | 2022-02-23 | 2022-05-27 | 广东农工商职业技术学院 | Method for preparing dihydromyricetin nano capsule based on vine tea extract |
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Application publication date: 20190222 |