CN109364287A - A kind of polysaccharide bleeding-stopping dressing and its preparation method and application - Google Patents
A kind of polysaccharide bleeding-stopping dressing and its preparation method and application Download PDFInfo
- Publication number
- CN109364287A CN109364287A CN201811166479.0A CN201811166479A CN109364287A CN 109364287 A CN109364287 A CN 109364287A CN 201811166479 A CN201811166479 A CN 201811166479A CN 109364287 A CN109364287 A CN 109364287A
- Authority
- CN
- China
- Prior art keywords
- oxidation
- polysaccharide
- bleeding
- chitosan
- stopping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Landscapes
- Health & Medical Sciences (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of polysaccharide bleeding-stopping dressings and its preparation method and application.The polysaccharide bleeding-stopping dressing is membrane-like or foam product as made of glycosaminoglycan and oxidation of polysaccharides cross-linking reaction；Wherein, the glycosaminoglycan is one or more of hyaluronic acid, chondroitin sulfate, heparin, chitosan, hydroxyethyl chitosan, hydroxypropyl chitosan, sulfonated chitosan, hydroxyl butyl chitosan, carboxymethyl chitosan, succinyl-chitosan or other chitosan derivatives；The oxidation of polysaccharides is one or more of oxidation of polysaccharides of the base containing dialdehyde, wherein the percentage that the sugar unit of the base containing dialdehyde accounts for the total sugar unit of polysaccharide molecule is 1%~70%.Polysaccharide bleeding-stopping dressing of the invention has significant anastalsis, and the significant bleeding stopping period for shortening the surface of a wound, dressing is non-toxic, has preferable biocompatibility.The medical instrument that can be used for preparing wound hemostasis, surgical hemostasis is developed as bleeding-stopping dressing medical instrument, is had a vast market foreground.
Description
Technical field
The present invention relates to a kind of polysaccharide biomaterials, more particularly to a kind of polysaccharide bleeding-stopping dressing and preparation method thereof and answer
With belonging to technical field of pharmaceutical biotechnology.
Background technique
The medical dressing of clinical use generally has aerogel dressing, film dressing, sponge dressing, pulvis dressing etc..Film
Dressing, sponge dressing, can be with flap coverage and microbial barriers, offer gas exchanges often with there is good mechanical performance.It is ideal
Bleeding-stopping dressing need quick-acting haemostatic powder, promoting healing performance, while there is good absorbency, histocompatbility, can cover
The surface of a wound protects the surface of a wound, accelerates wound healing.Multi-functional bleeding-stopping dressing is always the main research side of domestic and international hemostatic material
To.
Bleeding-stopping dressing clinically mainly has the mammal sources such as gelfoam, collagen protein sponge, Fibrin Glue
Bleeding-stopping dressing product and chitosan source bleeding-stopping dressing.The bleeding-stopping dressing of mammal source, due to that may be present
The propagation and immunogenicity risk of viral disease, attract attention on biological safety.Chitosan is a kind of natural polymer
Polycation polysaccharide, non-immunogenicity have no toxic side effect, and biological safety is good, while having hemostasis, antibacterial, promoting healing etc. and making
With with the superperformance for preparing bleeding-stopping dressing, antibacterial dressing, ulcer healing dressing.The anastalsis of chitosan is mainly source
The charge interaction between chitosan and red blood cell, is aggregated, to stop blooding.Wound is prepared as raw material using chitosan to stop
Blood, antibacterial, wound healing material have been reported.These reports are usually that chitosan is made to chitosan not soluble in water
Powder, chitosan film, chitosan sponge, chitin fiber and fabric etc., but since the functional effect of chitosan itself is limited, preparation
Dressing effect it is not satisfactory, need by chitosan and it is other be used in combination with other materials, to improve the comprehensive performance of dressing.
Summary of the invention
The object of the present invention is to provide a kind of polysaccharide bleeding-stopping dressings and its preparation method and application, to meet the prior art
The demand.
A kind of polysaccharide bleeding-stopping dressing, it is characterized in that membrane-like or sea as made of glycosaminoglycan and oxidation of polysaccharides cross-linking reaction
Continuous shape product；Wherein, the glycosaminoglycan is hyaluronic acid, chondroitin sulfate, heparin, chitosan, hydroxyethyl chitosan, hydroxyl
Propyl chitosan, sulfonated chitosan, hydroxyl butyl chitosan, carboxymethyl chitosan, succinyl-chitosan or other chitosans spread out
One or more of biology；The oxidation of polysaccharides is one or more of oxidation of polysaccharides of the base containing dialdehyde, wherein containing double
The percentage that the sugar unit of aldehyde radical accounts for the total sugar unit of polysaccharide molecule is 1%~70%.
The preparation method of above-mentioned polysaccharide bleeding-stopping dressing, it is characterized in that the following steps are included: with sterile water or sterile physiological salt
Water prepares the oxidation of polysaccharides glue that concentration expressed in percentage by weight is 0.5~20%, 0.5~20% glycosaminoglycan glue respectively；It will oxidation
Polysaccharide glue is mixed with glycosaminoglycan glue, is quickly stirred evenly, by obtained mixing polysaccharide glue quickly in glass plate or
Prolong stream and knifing, natural drying or heat drying on stainless steel flat plate or polystyrene plate, obtains membrane-like polysaccharide bleeding-stopping dressing；
Or quickly strike off obtained mixing polysaccharide glue in stainless steel or plastic mould, vacuum freeze drying obtains spongy polysaccharide
Bleeding-stopping dressing.
Above-mentioned polysaccharide bleeding-stopping dressing is preparing wound hemostasis, is applying in surgical hemostasis material, as wound hemostasis, operation
Bleeding-stopping dressing medical instrument is developed.
Polysaccharide bleeding-stopping dressing of the invention has a significant anastalsis, the significant bleeding stopping period for shortening the surface of a wound, dressing without
Toxicity has preferable biocompatibility.Therefore, polysaccharide bleeding-stopping dressing of the invention can prepare wound hemostasis, perform the operation and stop
It applies in the medical instrument of blood, is developed as bleeding-stopping dressing medical instrument, be used for wound hemostasis and surgical hemostasis, have wide
Wealthy market prospects.
Specific embodiment
Below by specific embodiment, present invention be described in more detail.
One, the preparation of oxidation of polysaccharides
Embodiment 1: the preparation of oxidized starch
It weighs starch 15g, is added in 500ml Erlenmeyer flask, add water 200ml, heating stirring dissolution, with 5%(weight percent,
Oxidant NaIO is added to pH5.5 in aqueous acetic acid tune pH value similarly hereinafter)43.5g, sealing are protected from light stirring oxidation at room temperature
Reaction is for 24 hours.Reaction solution is fitted into the bag filter that molecular cut off is 3000 dalton, is protected from light, distills at 4 DEG C by end of reaction
Water is dialysed for 24 hours, wherein every 6h replacement distilled water is primary.After dialysis, the liquid in bag filter is freezed in freeze drier
Dry 48h, is made the oxidized starch 13.5g of the base containing dialdehyde, and double percent aldehydes are that the bis- percent aldehydes of 12.5%(are
Refer to that the sugar unit of the base containing dialdehyde in polysaccharide molecule accounts for the percentage of the total sugar unit of polysaccharide molecule, similarly hereinafter).
Embodiment 2: the preparation of oxidated carboxymethyl cellulose
Carboxymethyl cellulose powder 15g is weighed, is added in 1000ml Erlenmeyer flask, adds water 800ml, stirring and dissolving, with 5% vinegar
Oxidant KIO is added to pH5.5 in aqueous acid tune pH value48g, sealing are protected from light stirring oxidation reaction 60h at room temperature.Reaction
It finishes, is added with stirring 95% ethanol water of 4 times of volumes, precipitate, sediment filters, and is washed with 95% ethanol water
3 times, the oxidated carboxymethyl cellulose 12.1g of the base containing dialdehyde is made in dehydrated alcohol dehydration, vacuum drying, and dialdehyde base percentage contains
Amount is 62.5%.
Embodiment 3: the preparation of alginic acid is aoxidized
Sodium alginate 15g is weighed, is added in 1000ml Erlenmeyer flask, adds water 800ml, stirring and dissolving, with the hydrochloric acid of 1mol/L
Oxidant KIO is added to pH5.0 in aqueous solution tune pH value49g, sealing are protected from light stirring oxidation reaction 36h at 4 DEG C.It has reacted
Finish, by reaction solution is fitted into molecular cut off be 3000 dalton bag filter in, be protected from light, at 4 DEG C distilled water dialyse for 24 hours, wherein
Every 6h replacement distilled water is primary.After dialysis, the liquid in bag filter is freeze-dried 48h in freeze drier, is made and contains
The oxidized sodium alginate 13g of dialdehyde base, double percent aldehydes are 48.5%.
Embodiment 4: the preparation of oxidized chondroitin sulphate
Chondroitin sulfate 12.0g is weighed, is added in 500ml Erlenmeyer flask, adds water 200ml, stirring and dissolving, with the salt of 1mol/L
Oxidant NaIO is added to pH5.0 in aqueous acid tune pH value41g, sealing are protected from light stirring oxidation reaction 18h at 4 DEG C.Reaction
Finish, by reaction solution be fitted into molecular cut off be 3000 dalton bag filter in, be protected from light, at 4 DEG C distilled water dialysis for 24 hours,
In every 6h replacement distilled water it is primary.After dialysis, the liquid in bag filter is freeze-dried 48h in freeze drier, is made
The oxidized chondroitin sulphate 11.3g of the base containing dialdehyde, double percent aldehydes are 2.5%.
Embodiment 5: the preparation of oxidized hyaluronic acid
It weighs hyaluronic acid 5g, is added in 1000ml Erlenmeyer flask, add water 500ml, stirring and dissolving, with 5%(weight percent,
Oxidant NaIO is added to pH5.5 in aqueous acetic acid tune pH value similarly hereinafter)42.5g, sealing are protected from light stirring oxidation at room temperature
Reaction is for 24 hours.Reaction solution is fitted into the bag filter that molecular cut off is 3000 dalton, is protected from light, distills at 4 DEG C by end of reaction
Water is dialysed for 24 hours, wherein every 6h replacement distilled water is primary.After dialysis, the liquid in bag filter is freezed in freeze drier
Dry 48h, is made the oxidized hyaluronic acid 3.9g of the base containing dialdehyde, and double percent aldehydes are 30.8%.
In above-described embodiment 1~5, the oxidation of polysaccharides of the base containing dialdehyde is prepared for using periodate oxidation method.Periodic acid oxygen
Change method can aoxidize a variety of biological polyoses, such as starch, acetate starch and other starch derivatives, carboxymethyl cellulose
Element, hydroxyethyl cellulose, hydroxypropyl cellulose and other cellulose derivatives, sodium alginate, fucoidin, agar polysaccharide, liver
Element, chondroitin sulfate, hyaluronic acid, chitosan, hydroxyethyl chitosan, hydroxypropyl chitosan, hydroxyl butyl chitosan, succinyl shell
Glycan, carboxymethyl chitosan and other chitosan derivatives, ethoxyl chitin, Hydroxypropyl chitosan, hydroxyl butyl chitin, carboxylic
Methyl chitin and other chitin derivativs, guar gum, hydroxypropyl guar gum and other guar derivatives, glucan are right
Other polysaccharide that the sugared acid anhydride of rotation and those skilled in the art can prepare, such as bletilla polysaccharide, aloe polysaccharide.Above-mentioned polysaccharide warp
After periodate oxidation, dialdehyde base is all contained in molecule, can be used as the raw material of bleeding-stopping dressing preparation of the present invention.
Two, the preparation of polysaccharide bleeding-stopping dressing
Raw material, container, vessel used in the preparation of the polysaccharide bleeding-stopping dressing etc. are germ-free condition, are prepared sterile
Under the conditions of complete, it is specific the preparation method is as follows:
Embodiment 6: the preparation of polysaccharide bleeding-stopping dressing 1
Oxidated carboxymethyl cellulose (double aldehyde group contents are 62.5%) 0.5g is weighed, is placed in the glass beaker of 100ml, is added molten
Agent sterile water 40ml stirs lower dissolution, obtains the oxidation of polysaccharides glue that weight percent concentration is 1.25%.It is poly- to weigh sulfonated shell
Sugared 0.8g, is placed in the glass beaker of 100ml, and solvent sterile water 40ml is added, and stirs lower dissolution, obtaining concentration expressed in percentage by weight is 2%
Glycosaminoglycan glue.40ml oxidation of polysaccharides glue is poured into 40ml glycosaminoglycan glue, equal proportion stirs evenly, and obtains
Mixing polysaccharide glue be quickly poured on clean glass plate (or stainless steel flat plate or polystyrene plate) and prolong stream and knifing,
Control glue dosage, make dry film with a thickness of 100~1000 μm, spontaneously dry (or heat drying), obtain membrane-like polysaccharide hemostasis
Dressing 1.
Embodiment 7: the preparation of polysaccharide bleeding-stopping dressing 2
Oxidation alginic acid (double aldehyde group contents are 48.5%) 2g is weighed, is placed in the glass beaker of 100ml, the sterile life of solvent is added
Salt water 40ml is managed, lower dissolution is stirred, obtains the oxidation of polysaccharides glue that weight percent concentration is 5%.Hydroxypropyl chitosan 4g, is placed in
In the glass beaker of 100ml, solvent sterile saline 40ml is added, stirs lower dissolution, obtains the ammonia that concentration expressed in percentage by weight is 10%
Quito carbohydrate gum liquid.40ml oxidation of polysaccharides glue is poured into 40ml glycosaminoglycan glue, equal proportion stirs evenly, and what is obtained is mixed
It closes polysaccharide glue and is quickly poured on clean glass plate (or stainless steel flat plate or polystyrene plate) and prolong stream and knifing, control
Glue dosage, make dry film with a thickness of 200~1500 μm, spontaneously dry (or heat drying), obtain membrane-like polysaccharide bleeding-stopping dressing
2。
Embodiment 8: the preparation of polysaccharide bleeding-stopping dressing 3
It weighs oxidized starch (double aldehyde group contents are 12.5%) 3.5g, weighs oxidized hyaluronic acid (double aldehyde group contents are 30.8%)
2.5g is placed in the glass beaker of 100ml, and sterile aqueous solvent 40ml is added, and stirs lower dissolution, obtaining weight percent concentration is
15% oxidation of polysaccharides glue.Hydroxyethyl chitosan 3g is weighed, succinyl-chitosan 3g is weighed, is placed in the glass beaker of 100ml
In, sterile aqueous solvent 40ml is added, stirs lower dissolution, obtains the glycosaminoglycan glue that concentration expressed in percentage by weight is 15%.40ml is aoxidized
Polysaccharide glue pours into 40ml glycosaminoglycan glue, and equal proportion stirs evenly, and obtained mixing polysaccharide glue is quickly poured into 50mm
It in stainless steel (or plastics) mold of × 50mm × 5mm, strikes off, is put into vacuum freeze drier and freezes 2~10 hours for -45 DEG C
Afterwards, vacuum freeze drying 36~48 hours obtain spongy polysaccharide bleeding-stopping dressing 3.
In the preparation of 6~8 polysaccharide bleeding-stopping dressing of above-described embodiment, the oxidation of polysaccharides, it is characterized in that base containing dialdehyde
One or more of oxidation of polysaccharides, wherein the sugar unit of the base containing dialdehyde account for the percentage of the total sugar unit of polysaccharide molecule be 1%~
70%.The oxidation of polysaccharides is oxidized starch derivative, oxycellulose derivative, oxidation marine algae polysaccharide derivant, oxidation
Glycosaminoglycan derivative, oxidized guar derivative, pullulan oxide derivative, oxidation xanthan derivatives, oxidation Portugal
Polysaccharid derivative, oxidized dextran derivative or other oxidation plant polyose derivatives；All contain in the molecule of oxidation of polysaccharides
Dialdehyde base can reach similar effect.The oxidized starch derivative refers to oxidized starch, oxidation acetate starch, or
Other oxidized starch derivatives；The oxycellulose derivative refers to oxidated carboxymethyl cellulose, oxidation hydroxy ethyl fiber
Element, oxidation hydroxypropyl cellulose or other oxycellulose derivatives；The oxidation marine algae polysaccharide derivant, refers to oxidation
Seaweed acid polysaccharide, oxidation fucoidin or oxidation agar polysaccharide；The amino-oxide group polysaccharide derivates, refer to heparin,
Oxidized chondroitin sulphate, oxidized hyaluronic acid, oxidation chitosan, oxidation hydroxyethyl chitosan, oxidation hydroxypropyl chitosan, oxidation
Hydroxyl butyl chitosan, oxidation succinyl-chitosan, oxidated carboxymethyl chitosan, oxidation ethoxyl chitin, oxidation hydroxypropyl first
Oxidation chitosan derivatives, the oxidation of shell element, oxidation hydroxyl butyl chitin, oxidated carboxymethyl chitin or other bases containing dialdehyde
Chitin derivativ；The oxidized guar derivative refers to oxidized guar, oxidation hydroxypropyl guar gum or other contains
Oxidized guar derivative；Other oxidation plant polyose derivatives refer to oxidation bletilla polysaccharide, oxidizing aloe polysaccharide,
Or and other oxidation plant polyoses.
In the preparation of 6~8 polysaccharide bleeding-stopping dressing of above-described embodiment, the glycosaminoglycan is that hyaluronic acid, sulfuric acid are soft
Ossein, heparin, chitosan, hydroxyethyl chitosan, hydroxypropyl chitosan, sulfonated chitosan, hydroxyl butyl chitosan, carboxymethyl shell
One or more of glycan, succinyl-chitosan or other chitosan derivatives.
Three, the application of polysaccharide bleeding-stopping dressing
Embodiment 9: application of the polysaccharide bleeding-stopping dressing as bleeding-stopping dressing
Using rat as experimental animal, hepatic tissue defect model is made, the anastalsis of bleeding-stopping dressing of the invention is evaluated.Examination
Rat 30 are tested, male and female simultaneous half, weight 200g ± 20g are divided into negative control group, 1 group of experiment, 2 groups, every group 10 of experiment.Examination
Before testing aseptically, bleeding-stopping dressing is cut into the size of 1cm × 1cm respectively, it is spare.It is infused using yellow Jackets abdominal cavity
Penetrate anesthetized rat, dosage 30mg/Kg.The notch for being about 3cm is cut in rat abdomen center, opens abdominal cavity, aseptic condition downstream
Liver lobus sinister is separated out, 5mm × 5mm × 2mm size hepatic tissue blocking is clamped in the middle part of liver with tissue forceps, causes hepatic tissue defect
Bleeding, medical cotton gauze are sucked blood, and stick the bleeding-stopping dressing that corresponding specification is 1cm × 1cm immediately.Negative control group rat
The bleeding surface of a wound is stopped blooding using hospital gauze, and 1 group of hemostasis attaches sterile film sheet polysaccharide bleeding-stopping dressing 2 in the rat bleeding surface of a wound and stops
Blood, 2 groups of hemostasis attach sterile spongy polysaccharide bleeding-stopping dressing 3 in the rat bleeding surface of a wound and stop blooding, and press 20 seconds respectively, observation wound
It face bleeding 20 seconds, repeats the above steps, observes bleeding, and record.Test result is shown in Table 1, the results showed that, hemostasis
1 group and 2 groups of hemostasis are with negative control group compared with, and bleeding-stopping dressing 2 and bleeding-stopping dressing 3 have significant haemostatic effect, wherein hemostasis is deposited
The haemostatic effect of material 3 is better than bleeding-stopping dressing 2, while the haemostatic effect of better than hospital gauze, shows bleeding-stopping dressing of the invention
With significant anastalsis.
The anthemorrhagic performance evaluation result of 1 bleeding-stopping dressing of table
| Group | Experimental animal quantity (only) | Bleeding stopping period (second) |
| Negative control group | 10 | > 180 |
| Test 1 group | 10 | 45-78 |
| Test 2 groups | 10 | 30-56 |
Polysaccharide bleeding-stopping dressing of the invention has preferable mechanical strength, flexibility, has significant anastalsis, can obviously contract
The bleeding stopping period of the short surface of a wound, dressing is non-toxic, has preferable biocompatibility.Therefore, polysaccharide bleeding-stopping dressing of the invention can
To be applied in the medical instrument for preparing wound hemostasis, surgical hemostasis, developed as bleeding-stopping dressing medical instrument, for creating
Wound hemostasis and surgical hemostasis, have a vast market foreground.
Claims (10)
1. a kind of polysaccharide bleeding-stopping dressing, it is characterized in that membrane-like or sponge as made of glycosaminoglycan and oxidation of polysaccharides cross-linking reaction
Shape product；Wherein, the glycosaminoglycan is hyaluronic acid, chondroitin sulfate, heparin, chitosan, hydroxyethyl chitosan, hydroxypropyl
Base enclosure glycan, sulfonated chitosan, hydroxyl butyl chitosan, carboxymethyl chitosan, succinyl-chitosan or other chitosan derivatives
One or more of object；The oxidation of polysaccharides is one or more of oxidation of polysaccharides of the base containing dialdehyde, wherein containing dialdehyde
The percentage that the sugar unit of base accounts for the total sugar unit of polysaccharide molecule is 1%~70%.
2. polysaccharide bleeding-stopping dressing as described in claim 1, it is characterized in that the oxidation of polysaccharides of the base containing dialdehyde, is oxidized starch
Derivative, oxycellulose derivative, oxidation marine algae polysaccharide derivant, amino-oxide group polysaccharide derivates, oxidized guar are derivative
Object, pullulan oxide derivative, oxidation xanthan derivatives, oxidized dextran derivative, oxidized dextran derivative,
Or one or more of other oxidation plant polyose derivatives.
3. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that the oxidized starch derivative refer to oxidized starch,
Aoxidize acetate starch or other oxidized starch derivatives.
4. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that the oxycellulose derivative refers to oxidation carboxylic
Methylcellulose, oxidation hydroxyethyl cellulose, oxidation hydroxypropyl cellulose or other oxycellulose derivatives.
5. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that the oxidation marine algae polysaccharide derivant refers to oxidation
Seaweed acid polysaccharide, oxidation fucoidin or oxidation agar polysaccharide.
6. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that the amino-oxide group polysaccharide derivates refer to oxidation
Heparin, oxidized chondroitin sulphate, oxidized hyaluronic acid, oxidation chitosan, oxidation hydroxyethyl chitosan, oxidation hydroxypropyl
Sugar, oxidation hydroxyl butyl chitosan, oxidation succinyl-chitosan, oxidated carboxymethyl chitosan, oxidation ethoxyl chitin, oxidation hydroxyl
Propyl chitin, the oxidation chitosan derivative for aoxidizing hydroxyl butyl chitin, oxidated carboxymethyl chitin or other bases containing dialdehyde
Object, oxidated chitin derivative.
7. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that the oxidized guar derivative refers to oxidation melon
That glue, oxidation hydroxypropyl guar gum or other containing oxidized guar derivative.
8. polysaccharide bleeding-stopping dressing as claimed in claim 2, it is characterized in that other oxidation plant polyose derivatives refer to
Aoxidize bletilla polysaccharide or oxidizing aloe polysaccharide.
9. the preparation method of the described in any item polysaccharide bleeding-stopping dressings of claim 1-8, it is characterized in that the following steps are included: with nothing
Bacterium water or sterile saline prepare the oxidation of polysaccharides glue that concentration expressed in percentage by weight is 0.5~20%, 0.5~20% ammonia respectively
Quito carbohydrate gum liquid；Oxidation of polysaccharides glue is mixed with glycosaminoglycan glue, is quickly stirred evenly, the mixing polysaccharide glue that will be obtained
Quickly prolong stream and knifing, natural drying or heat drying on glass plate or stainless steel flat plate or polystyrene plate, obtains film
Sheet polysaccharide bleeding-stopping dressing；Or obtained mixing polysaccharide glue is quickly struck off in stainless steel or plastic mould, vacuum refrigeration
It is dry, obtain spongy polysaccharide bleeding-stopping dressing.
10. the described in any item polysaccharide bleeding-stopping dressings of claim 1-8, prepare wound hemostasis, answering in surgical hemostasis material
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811166479.0A CN109364287A (en) | 2018-10-08 | 2018-10-08 | A kind of polysaccharide bleeding-stopping dressing and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811166479.0A CN109364287A (en) | 2018-10-08 | 2018-10-08 | A kind of polysaccharide bleeding-stopping dressing and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109364287A true CN109364287A (en) | 2019-02-22 |
Family
ID=65403101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811166479.0A Pending CN109364287A (en) | 2018-10-08 | 2018-10-08 | A kind of polysaccharide bleeding-stopping dressing and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109364287A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908394A (en) * | 2019-02-26 | 2019-06-21 | 南京理工大学 | Degradable medical antibacterial leakage protection film and preparation method thereof |
| CN110975001A (en) * | 2019-12-09 | 2020-04-10 | 军事科学院系统工程研究院卫勤保障技术研究所 | Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof |
| CN111632188A (en) * | 2020-06-09 | 2020-09-08 | 湖北精耕生物工程有限公司 | Seaweed polysaccharide composite biological matrix dressing and preparation method thereof |
| CN113024847A (en) * | 2021-03-19 | 2021-06-25 | 中国科学院宁波材料技术与工程研究所 | Application of natural polysaccharide hydrogel in hemostasis field |
| CN113350568A (en) * | 2020-12-31 | 2021-09-07 | 孛朗孚(杭州)生物科技有限公司 | Biocompatible polymer dressing based on gelatin |
| CN114805628A (en) * | 2022-06-21 | 2022-07-29 | 四川大学 | Method for preparing hemostatic material from bletilla striata polysaccharide modified collagen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030064089A1 (en) * | 2001-09-04 | 2003-04-03 | Vijay Kumar | Regenerated cellulose and oxidized cellulose membranes as potential biodegradable platforms for drug delivery and tissue engineering |
| CN1434072A (en) * | 2003-01-27 | 2003-08-06 | 武汉大学 | High-strength antimicrobial dialdehyde starch crosslinked chitosan membane and preparation method and use thereof |
| CN101209354A (en) * | 2007-12-25 | 2008-07-02 | 青岛博益特生物材料有限公司 | Medical blood-stopping healing agent for wound-surface and using thereof |
-
2018
- 2018-10-08 CN CN201811166479.0A patent/CN109364287A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030064089A1 (en) * | 2001-09-04 | 2003-04-03 | Vijay Kumar | Regenerated cellulose and oxidized cellulose membranes as potential biodegradable platforms for drug delivery and tissue engineering |
| CN1434072A (en) * | 2003-01-27 | 2003-08-06 | 武汉大学 | High-strength antimicrobial dialdehyde starch crosslinked chitosan membane and preparation method and use thereof |
| CN101209354A (en) * | 2007-12-25 | 2008-07-02 | 青岛博益特生物材料有限公司 | Medical blood-stopping healing agent for wound-surface and using thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109908394A (en) * | 2019-02-26 | 2019-06-21 | 南京理工大学 | Degradable medical antibacterial leakage protection film and preparation method thereof |
| CN110975001A (en) * | 2019-12-09 | 2020-04-10 | 军事科学院系统工程研究院卫勤保障技术研究所 | Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof |
| CN110975001B (en) * | 2019-12-09 | 2022-04-12 | 军事科学院系统工程研究院卫勤保障技术研究所 | Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof |
| CN111632188A (en) * | 2020-06-09 | 2020-09-08 | 湖北精耕生物工程有限公司 | Seaweed polysaccharide composite biological matrix dressing and preparation method thereof |
| CN113350568A (en) * | 2020-12-31 | 2021-09-07 | 孛朗孚(杭州)生物科技有限公司 | Biocompatible polymer dressing based on gelatin |
| CN113024847A (en) * | 2021-03-19 | 2021-06-25 | 中国科学院宁波材料技术与工程研究所 | Application of natural polysaccharide hydrogel in hemostasis field |
| CN114805628A (en) * | 2022-06-21 | 2022-07-29 | 四川大学 | Method for preparing hemostatic material from bletilla striata polysaccharide modified collagen |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109364287A (en) | A kind of polysaccharide bleeding-stopping dressing and its preparation method and application | |
| Hu et al. | Rational design and latest advances of polysaccharide-based hydrogels for wound healing | |
| Lu et al. | Mussel-inspired blue-light-activated cellulose-based adhesive hydrogel with fast gelation, rapid haemostasis and antibacterial property for wound healing | |
| CN109453417A (en) | A kind of polysaccharide burn dressing and its preparation method and application | |
| Wang et al. | Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata polysaccharide composite hemostatic sponges | |
| Zhu et al. | Recent progress of polysaccharide‐based hydrogel interfaces for wound healing and tissue engineering | |
| Xie et al. | Carboxymethyl konjac glucomannan-crosslinked chitosan sponges for wound dressing | |
| CN100443123C (en) | Medical chitosan dressing and its application | |
| Yu et al. | Hemostatic materials in wound care | |
| Fan et al. | Rapid hemostatic chitosan/cellulose composite sponge by alkali/urea method for massive haemorrhage | |
| CN101053669B (en) | Water soluble chitosan-based hemostatic wound-healing marine sponge and its preparation method and application | |
| Jing et al. | Marine polysaccharides: Green and recyclable resources as wound dressings | |
| Yang et al. | Polysaccharide-based multifunctional hydrogel bio-adhesives for wound healing: A review | |
| US11787922B2 (en) | Hydrophobically modified chitosan compositions | |
| CN109260504A (en) | A kind of amino sugar hemostasis medical material and its preparation method and application | |
| CN109289074A (en) | A kind of wound healing material and its preparation method and application | |
| Tarrahi et al. | The latest achievements in plant cellulose-based biomaterials for tissue engineering focusing on skin repair | |
| WO2001034214A1 (en) | Use of soluble cellulose derivative having been made hardly soluble in water and process for producing the same | |
| Liu et al. | Chitosan-based hemostatic sponges as new generation hemostatic materials for uncontrolled bleeding emergency: Modification, composition, and applications | |
| Zheng et al. | A novel pullulan oxidation approach to preparing a shape memory sponge with rapid reaction capability for massive hemorrhage | |
| Sari et al. | A novel composite membrane pectin from Cyclea Barbata Miers blend with chitosan for accelerated wound healing | |
| CN107405421A (en) | With hemostat devices for improving property and preparation method thereof | |
| Zhou et al. | Protocatechuic acid-mediated injectable antioxidant hydrogels facilitate wound healing | |
| Tan et al. | A self-gelling powder based on polyacrylic acid/polyacrylamide/quaternate chitosan for rapid hemostasis | |
| CN114129767B (en) | Surface-sealing soft tissue wound surface protective adhesive and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190222 |
|
| WD01 | Invention patent application deemed withdrawn after publication |