CN109364130A - A kind of spina gleditsiae adulterant is preparing the application in antioxidant - Google Patents
A kind of spina gleditsiae adulterant is preparing the application in antioxidant Download PDFInfo
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- CN109364130A CN109364130A CN201811518828.0A CN201811518828A CN109364130A CN 109364130 A CN109364130 A CN 109364130A CN 201811518828 A CN201811518828 A CN 201811518828A CN 109364130 A CN109364130 A CN 109364130A
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- spina gleditsiae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
Abstract
The present invention provides a kind of spina gleditsiae adulterants to prepare the application in antioxidant, by the detection of the antioxidant activity for spina gleditsiae adulterant, spina gleditsiae adulterant is compared to substances such as rutin, haw thorn extracts, it is even more preferably active to show comparable activity, DPPH free radical scavenging activity can be to 60%~75%, ORAC value can reach 1.20~1.30, provides new strategy to research and develop relevant preparation, drug etc., has a good application prospect and higher application value.
Description
Technical field
The invention belongs to field of medicinal chemistry, it is related to a kind of spina gleditsiae adulterant and is preparing the application in antioxidant.
Background technique
" Chinese Pharmacopoeia " (version in 2015) record spina gleditsiae has the effect of detumescence and poison retention, apocenosis, desinsection；For ulcer
From the beginning of or purulence at not bursting；Castor is controlled outside.In recent years, with the development and utilization of drug, the market demand of spina gleditsiae surges.And
Currently, the produced spina gleditsiae quality of Gleditsia officinalis of different regions growth is irregular, the quil of a lot of other plants is also mingled in soap
It is sold among the thorn of angle.Common adulterant has mountain spina gleditsiae (Gleditsia japonica Miq.), wild spina gleditsiae (Gleditsia
Microphylla Gordon ex Y.T.Lee) and wild jujube thorn (Ziziphus jujuba Mill.var.spinosa
(Bunge) Hu ex H.F.Chow) etc., wherein again most commonly seen with mountain spina gleditsiae and wild spina gleditsiae.
And the generation of above-mentioned disease related with spina gleditsiae adulterant is largely all lost with the metabolism of activity in vivo oxygen
It adjusts related.For this purpose, external using DPPH radicals scavenging method (DPPH method) and two kinds of oxygen radical removing ability method (ORAC method)
Antioxidant activity evaluation method.
Although most of free-radical chemistry properties are extremely active, the service life is extremely short, also there is the chemical property of a small number of free radicals
Sufficiently stable, DPPH (1,1-diphenyl-2-picrylhydrazyl i.e. 1,1- diphenyl -2- picryl phenylhydrazine) free radical is exactly
One of them.Its stability and makes its nitrogen that is clipped in the middle mainly from the spatial obstacle of Resonance Stabilization action and three phenyl ring
Unpaired electron on atom cannot play its due electronics and act in pairs.The ethanol solution of DPPH is purple, in 517nm
There is strong absorption at place.In the presence of having free radical scavenger, the single electron of DPPH is paired and solution colour is made to shoal, in maximum
Absorbance at absorbing wavelength becomes smaller, and the degree that the degree that becomes smaller of absorbance and free radical are removed is in a linear relationship.Cause
This, the absorbance at this wavelength can be used to detect the removing situation of free radical, thus the oxidation resistance of evaluation test sample.
It is AH+DPPH → DPPH-H+A, effective phenol hydroxyl in scavenging capacity and antioxidant molecule that antioxidant, which functions mode,
The number of base and the stability of the antioxidant free radical (A) newly formed are related.Blois in 1958 is applied to anti-oxidant
The screening study of agent, hereafter lot of domestic and international scholar has studied the property that substance removes free radical with the method.
Fluorescent material uranine yellow can emit 527nm fluorescence under the excitation of 485nm light.AAPH[2,2'-azobis(2-
Amidinopropane) dihydrochloride, 2,2'- azo (two narrow base propane) dichloride hydrogen] it is releasable in aqueous solution
Peroxy radical (ROO), and uranine yellow can be aoxidized, so that its fluorescent characteristic is disappeared.It, can be in the presence of antioxidant
Uranine yellow competitive oxidation agent slows down the speed of its fluorescence recession.According to this characteristic, the oxygen radical of sample can be measured
Scavenging capacity (oxygen radical absorbance capacity, ORAC)].ORAC method is a kind of total antioxygen of measurement substance
The method of ability, with the water-soluble analogues-Trolox of alpha-tocopherol a kind of (6-hydroxy-2,5,7,8-
Tetramethylchroman-2-carboxylic acid, 6- hydroxyl -2,5,7,8- tetramethyl benzodihydropyran -2- carboxylic
Acid) it is used as standard control.This method is capable of providing stably and controllable free radical source, is suitable for hydrophily and lipophilicity chemical combination
Object is accurate with fluorescent quantitation result and can accomplish micro and high-throughput automation detection.Glaz and Ghiselli et al. are utilized should
After method carries out antioxidant activity research, by a series of improvement, this method has been widely used for evaluation biological products
With the oxidation resistance of food.
Spina gleditsiae adulterant equally has some pharmacological effects similar to spina gleditsiae.
CN104087487A discloses a kind of brewage process of spina gleditsiae wine, and using spina gleditsiae as raw material, sweet potato is matrix, warp
Cross feedstock processing, immersion, ultra-fine grinding, sweet potato processing, raw material mixing, dosing, post-fermentation, squeeze and filter, wine liquid mixing etc.
Step is process；The present invention uses the brewage process for combining raw material soaking with fermentation, can not only improve the benefit of raw material
With rate, and make that finished product spina gleditsiae wine nutriment is more balanced, mellow in taste, long times of aftertaste, at the same also have detumescence and apocenosis,
The various health care functions such as dispelling, collateral-activating, clearing heat and detoxicating.The method discloses spina gleditsiae and combines the use of other products, has certain guarantor
Strong effect.
CN102940672B discloses a kind of application of spina gleditsiae general flavone in terms of preparation prevents and treats tumour medicine,
More particularly to spina gleditsiae general flavone as gap junctional intercellular communication reinforcing agent, answering in terms of preparation prevents and treats tumour medicine
With the especially application in terms of tumor prevention, chemotherapy of tumors synergy, hyperblastosis, such as prevention chemical carcinogenesis, raising are anti-tumor
It treats index, reversing tumor chemotherapy resistance, prevent the proliferation of mammary gland and hyperplasia of prostate etc..Spina gleditsiae is disclosed in this application anti-swollen
The effect of in terms of tumor.
CN107383151A disclose in a kind of spina gleditsiae the Pentacyclic triterpene saponins compounds of anti-breast cancer activity and its
Extracting method, the method give spina gleditsiae and can be applicable to anti-breast cancer in terms of potential value.
Currently, the application about spina gleditsiae adulterant in terms of anti-oxidant, does not there is any relevant research and relevant report also
It leads.
Summary of the invention
The purpose of the present invention is to provide a kind of spina gleditsiae adulterants to prepare the application in antioxidant, to promote to expand soap
The application field of adulterant is pierced at angle, promotes the application value of spina gleditsiae adulterant.
To achieve this purpose, the present invention adopts the following technical scheme:
The present invention provides a kind of spina gleditsiae adulterants to prepare the application in antioxidant.
The present invention unexpectedly studies discovery spina gleditsiae adulterant, has stronger antioxidation, can be used as a kind of anti-oxidant
Agent application, by the detection of the antioxidant activity for spina gleditsiae adulterant, spina gleditsiae adulterant is compared to rutin, haw thorn extract etc.
Substance, it is even more preferably active to show comparable activity, provides new plan to research and develop relevant preparation, drug etc.
Slightly.
In current research, the effect about spina gleditsiae adulterant in terms of anti-oxidant is not reported, also, compared to independent
Flavonoid substances, have superior antioxidant activity.
Preferably, the spina gleditsiae adulterant includes any one in mountain spina gleditsiae, wild spina gleditsiae or wild jujube thorn.
Preferably, the DPPH free radical scavenging activity of the antioxidant is 60%~75%, such as can be 60%,
62%, 64%, 65%, 66%, 70%, 71%, 72%, 73%, 74% or 75% etc..
Preferably, the ORAC value of the antioxidant is 1.20~1.30, for example, can be 1.20,1.21,1.22,
1.23,1.24,1.25,1.26,1.27,1.28,1.29,1.30 etc..
ORAC value of the present invention refers to that in the presence of antioxidant, protected integral area is (i.e. glimmering on fluorescence decline curve
Blank area under the curve of the light fall-off curve lower integral Area subtraction without antioxidant) size, it is specific as shown in Figure 1.It is different
The protected area equivalent of antioxidant is compared at the protected area of 1 μM of Trolox.Therefore, 1 ORAC unit definition
Are as follows: final concentration of 1 μM of Trolox corresponding protection integral area on fluorescence decline curve.
Preferably, the antioxidant is as anti-oxidation medicine.
In the present invention, antioxidant can be developed further into as relevant anti-oxidation medicine.
Preferably, the anti-oxidation medicine further includes pharmaceutically acceptable auxiliary material.
Anti-oxidation medicine provided by the invention is that antioxidant is prepared with optionally auxiliary material.
Preferably, the pharmaceutically acceptable auxiliary material include filler, adhesive, disintegrating agent, lubricant, glidant,
It is any one in hypertensor, effervescent agent, surfactant, film forming agent, toner, corrigent, preservative, dispersing agent or aromatic
Kind or at least two combination.
Filler can be the auxiliary material that starch, carbohydrate, cellulose family or inorganic salts etc. have filling effect.Adhesive
It can be microcrystalline cellulose, gelatin, carbohydrate, polyethylene glycol, polyvinylpyrrolidone, pregelatinized starch, hydroxypropyl cellulose
Or the auxiliary material that there is hydroxypropyl methyl cellulose etc. raising viscosity to be easy to preparations shaping effect.Disintegrating agent may include starch hydroxyl second
Sour sodium, sodium carboxymethylcellulose, calcium carboxymethylcellulose, croscarmellose sodium, crosslinked polyvinylpyrrolidone, poly- second
Alkene pyrrolidone, methylcellulose, microcrystalline cellulose, the hydroxypropyl cellulose of lower alkyl groups substitution, starch, pregelatinated form sediment
Powder or sodium alginate etc. have the auxiliary material of calving disaggregation.Lubricant can be fatty acid magnesium, calcium stearate, zinc stearate, tristearin firmly
There is mixture of one or more of acyl fumaric acid sodium and magnesium stearate, lauryl sodium sulfate etc. lubrication to make
Auxiliary material.Glidant, which can be silica or talcum powder etc., has the auxiliary material for improving mobility effect.Surfactant can
To be that lecithin or fatty glyceride etc. have the auxiliary material for increasing solubility effect.Those skilled in the art can be according to being actually subjected to
The dosage form of preparation selects suitable pharmaceutically acceptable auxiliary material to be processed.
In addition, the present invention also found that spina gleditsiae adulterant also has certain nitric oxide inhibiting effect by research, suppression
Making nitric oxide production inhibiting rate can reach 60% or more, have comparable activity with resveratrol.
Compared with the existing technology, the invention has the following advantages:
The present invention, which unexpectedly studies discovery spina gleditsiae adulterant, has stronger antioxidation, can be used as a kind of antioxidant
Using by the detection of the antioxidant activity for spina gleditsiae adulterant, spina gleditsiae adulterant is compared to objects such as rutin, haw thorn extracts
Matter, shows that comparable activity is even more preferably active, and DPPH free radical scavenging activity can be reachable to 60%~75%, ORAC value
To 1.20~1.30, new strategy is provided to research and develop relevant preparation, drug etc..
Detailed description of the invention
Fig. 1 is the schematic diagram of protected integral area on fluorescence decline curve in the present invention.
Specific embodiment
The technical scheme of the invention is further explained by means of specific implementation.Those skilled in the art should be bright
, the described embodiments are merely helpful in understanding the present invention, should not be regarded as a specific limitation of the invention.
In following embodiment of the present invention, for the sample of use as cited by the following table 1, serial number is opposite with the serial number in embodiment
It answers.
Table 1
Above-mentioned sample extracts preparation by the following method, obtains test sample before being tested.
Sample powder (crossing No. 3 sieves) about 1.0g, it is accurately weighed, it sets in stuffed conical flask, 70% ethanol solution 10mL is added,
Close plug ultrasonic treatment (power 250W, frequency: 40kHz) 2 times, each 30min, filtering, merging filtrate set 50 DEG C of water in evaporating dish
Bath is waved to 1~2mL of liquor capacity, and residue adds 70% ethanol solution to be dissolved in 5mL measuring bottle, is diluted to quarter with 70% ethanol solution
Degree, shakes up, through 0.45 μm of filtering with microporous membrane to get test solution.
Embodiment 1
The present embodiment measures DPPH free radical scavenging activity by the following method
(1) preparation of DPPH solution and test solution
DPPH is made into 2 × 10 with dehydrated alcohol-4The solution of M is placed in 4 DEG C and is kept in dark place.Each monomeric compound and the positive
Medicine is made into the stoste of 1mg/mL with dehydrated alcohol, is diluted to required concentration with dehydrated alcohol before test.
(2) active measurement
Reference literature experimental method is simultaneously improved, by the test solution 100 μ L and 2 × 10 of various concentration-4M
100 μ L of DPPH solution is added in each hole of 96 orifice plates, while to be not added DPPH's (replacing DPPH with 100 μ L dehydrated alcohols)
Each concentration of test solution is as control to eliminate interference of the test sample intrinsic colour to test result, and it is right to set DPPH feminine gender
According to (replacing test sample with 100 μ L dehydrated alcohols), every group sets 4 multiple holes in parallel.96 orifice plates are put into microplate reader, are shaken
1min, and in saving (room temperature is protected from light) under this condition, its absorbance OD value at 517nm is tested after 30min, by following public
The free radical scavenging activity of formula calculating test sample.
Free radical scavenging activity=[ODDPPH·control-(ODsample-ODsample control)]/ODDPPH·control× 100%
Wherein: ODDPPH·controlFor the average value of DPPH negative control group OD value；
DsampleFor the average value of sample sets OD value；
ODsample controlFor the average value of sample ethanol control group OD value.
(3) the tested results of free radical scavenging ability
The ability for removing DPPH free radical under 50 μ g/mL concentration to each extract using above-mentioned experimental method is surveyed
Examination, while using flavone compound rutin (concentration is 10 μ g/mL) and haw thorn extract (concentration is 40 μ g/mL) as the positive
Control is higher than 50% with free radical scavenging activity and thinks active.Experimental result is as shown in table 2 (wherein in sample number into spectrum and table 1
Sample representated by sample number into spectrum is corresponding):
Table 2
| Sample number into spectrum | Clearance rate |
| 1 | 60.1% |
| 2 | 62.1% |
| 3 | 65.6% |
| 4 | 61.7% |
| 5 | 68.7% |
| 6 | 65.3% |
| 7 | 64.4% |
| Haw thorn extract | 52.7% |
| Rutin | 63.2% |
Embodiment 2
The present embodiment measures oxygen radical removing ability by the following method
(1) preparation of reaction reagent and test solution
Kaliumphosphate buffer (the 75mM KH of uranine yellow 75mM2PO4、75mM K2HPO4) it is made into 63 μM of deposit
Liquid is placed in 4 DEG C and is kept in dark place, and dilutes 100 times with the buffer before test.AAPH is before experiment with the kaliumphosphate buffer of 75mM
It is made into the solution of 18.3mM.Trolox is made into 10 μM of stoste with the kaliumphosphate buffer of 75mM, dilute with the buffer before test
It is interpreted into required concentration.Each monomeric compound and positive drug VC are made into the stoste of 100mM with the kaliumphosphate buffer of 75mM, test
It is preceding to be diluted to required concentration with the buffer.
(2) active measurement
The ORAC that this experiment uses measures the method referring to Ronald L.Prior and is improved.It is each to test 96 orifice plates
The 20 μ L of testing sample solution of various concentration is added in hole, it is (whole to add 20 140 μ L of μ L and AAPH of 75mM kaliumphosphate buffer
Concentration 12.8mM), it is eventually adding 20 μ L (final concentration 63nM) of uranine yellow starting reaction, 96 orifice plates are placed in luciferase rapidly
Start to measure in mark instrument (37 DEG C of preset temperature), every group sets 3 multiple holes in parallel.Using dynamic behavior, every 2min measures one
Point, until fluorescence-intensity decay is zero.
(3) experimental result is indicated with antioxidant ability index, it refers in the presence of antioxidant, on fluorescence decline curve
The size of protected integral area (i.e. blank area under the curve of the fluorescence decline curve lower integral Area subtraction without antioxidant),
As shown in Figure 1, only schematic diagram.The protected area equivalent of different antioxidants is carried out at the protected area of 1 μM of Trolox
Compare.Therefore, 1 ORAC unit definition are as follows: final concentration of 1 μM of Trolox corresponding protection integral on fluorescence decline curve
Area.
Using above-mentioned experimental method, to each extract, the oxygen radical removing ability under 50 μ g/mL concentration is tested,
Use flavone compound rutin (concentration be 30 μ g/mL) as positive control simultaneously, be equivalent to antioxidant ability index or
Think active better than rutin.Concrete outcome is as shown in table 3:
Table 3
Embodiment 3
Each component content that the present embodiment passes through the spina gleditsiae adulterant that sample number into spectrum in liquid chromatography for measuring table 1 is 1-7
Using Agilent ZORBAX SB-Aq C18 (250mm × 4.6mm, 5 μm) chromatographic column, mobile phase is methanol-
0.1% acetic acid solution gradient elution, Detection wavelength 254nm, 35 DEG C of column temperature, flow velocity 1.0mL/min, when sample volume is 10 μ L
Gained chromatogram separating degree and peak type are preferable, and chromatographic peak number is more, are evenly distributed.Chromatogram is recorded to 2 times of retention times
(180min) occurs without chromatographic peak after 90min, therefore analysis time is 90min, records peak area.The results are shown in Table 4 (wherein
Sample number into spectrum is corresponding with sample representated by sample number into spectrum in table 1):
Table 4
As shown in Table 4, texifolin, young fustic equal size are higher in spina gleditsiae adulterant, the presence of these substances, significantly
Promote the antioxidant activity of spina gleditsiae.
By the test result of embodiment 1-3 it is found that spina gleditsiae adulterant active material rich in, between each active material
Mutually promote so that spina gleditsiae adulterant antioxidant activity with higher, compared to rutin, haw thorn extract etc., there is phase
When the even higher activity of activity.
Embodiment 4
The present embodiment measures by the following method inhibits rat macrophage NO release activity
Since NO is extremely unstable, it is oxidized to soon Property is stablized, therefore can pass through measurementIndirectly
Reflect the production quantity of NO.The application is using in Griess method measurement solutionContent, principle be acid condition under,With P-aminobenzene-sulfonamide (Sulfanilamide) diazotising, then with hydrochloric acid N-1- naphthalene-ethylenediamine (N-1-
Naphthylethylene-diamine dihydrochloride) coupling reaction occurs, the azo dyes of aubergine is generated,
There is maximum absorption spectrum at 570nm, its absorbance can be measured with enzyme-linked immunosorbent assay instrument.The measurement of Griess methodWith height
The characteristics of sensitivity, high accuracy and reproducibility, and method is simple and effective, is most common measurementContent, measurement
The method of NO level.
(1) preparation of the culture of cell and test sample, reaction reagent
264.7 rat macrophage of RAW (Abelson Leukemia virus transformed) is derived from U.S. ATCC
(America Tissue Culture Collection).At 37 DEG C, 5%CO2Damp and hot incubator in, with containing 10%FBS
HAM ' s F12 culture medium culture.
HAM ' s F12 culture medium (Sigma, N4888): glutamic acid (Sigma) and 50mL tire ox blood are added in 500mL
Clearly.
IFN-γ: Genzyme/Techne company.
Sulfanilamide, N-1-naphthylethylene-diamine dihydrochloride, MTT:Wako are public
Department.
Griess reagent: hydrochloric acid N-1- naphthalene-ethylenediamine (N-1-naphthylethylene-diamine
Dihydrochloride) 5mg water for injection 5mL dissolves；P-aminobenzene-sulfonamide (Sulfanilamide) 50mg is added 250
5mL is diluted to water for injection after μ L phosphoric acid.
Enzyme-linked immunosorbent assay instrument: Model 3550Microplate Reader, BIO-RAD.
Each monomeric compound is made into the test liquid that concentration is respectively 100mM, 30mM, 10mM and 3mM, low-temperature dark with DMSO
It saves.
(2) active measurement
RAW264.7 cell (is derived from U.S. ATCC), adjustment cell concentration to 1.2 × 106A/mL is added with every 200 μ L of hole
Enter in 96 well culture plates, 5%CO2, 37 DEG C, cultivate 2 hours.Lipopolysaccharides (LPS, 10 μ g/mL) 2 μ L, interferon-γ is added
(IFN-γ, 33ng/mL) 2 μ L, 0.4 μ L of sample DMSO solution (make the final concentration of 100ng/mL of LPS, IFN-γ is final concentration of
0.33ng/mL, the DMSO of sample dissolution are 0.2%), to cultivate 16 hours relative to the content of culture medium.Then take supernatant 100
Each 50 μ L of Griess reagent mixed up in advance is added in μ L, measures absorbance in 550nm with enzyme-linked immunosorbent assay instrument.And not add
Sample and inducer lipopolysaccharides, interferon-γ cell liquid as blank control, not to be loaded the cell liquid of product as negative right
According to.The cytotoxicity of each monomeric compound is tested with mtt assay simultaneously.Macrophage NO release inhibiting rate is calculated as follows
And cell survival rate:
Cell survival rate=sample sets OD average value ÷ negative control group OD cell mean × 100%
(3) experimental result
Using above-mentioned experimental method, to each extract object, the nitric oxide rejection ability under 100 μ g/mL concentration is surveyed
Examination, as positive control drug, its NO that there is preferable inhibition iNOS to induce of document report discharges to live simultaneous selection resveratrol
Property, it is 62.3% that in 50 μ g/mL, NO, which discharges inhibiting rate, is higher than 50% with NO release inhibiting rate and thinks active.It obtains
The results are shown in Table 5 (wherein sample number into spectrum is corresponding with sample representated by sample number into spectrum in table 1):
Table 5
| Sample number into spectrum | Inhibiting rate (%) |
| 1 | 68.1 |
| 2 | 65.2 |
| 3 | 63.3 |
| 4 | 60.3 |
| 5 | 62.4 |
| 6 | 63.7 |
| 7 | 61.1 |
| Resveratrol | 62.3 |
By the result of table 5 it is found that spina gleditsiae adulterant nitric oxide inhibitory activity also with higher, a kind of new to develop
The nitric oxide inhibitor of type provides thinking.
The Applicant declares that the present invention is explained by the above embodiments, spina gleditsiae adulterant of the invention is preparing antioxidant
In application, but the invention is not limited to above-mentioned method detaileds, that is, do not mean that the present invention must rely on above-mentioned method detailed
It could implement.It should be clear to those skilled in the art, any improvement in the present invention, to each raw material of product of the present invention
Equivalence replacement and addition, the selection of concrete mode of auxiliary element etc., all fall within protection scope of the present invention and the open scope
Within.
Claims (7)
1. a kind of spina gleditsiae adulterant is preparing the application in antioxidant.
2. application according to claim 1, which is characterized in that the spina gleditsiae adulterant includes mountain spina gleditsiae, wild spina gleditsiae
Or any one in wild jujube thorn.
3. application according to claim 1 or 2, which is characterized in that the DPPH free radical scavenging activity of the antioxidant is
60%~75%.
4. application according to any one of claim 1-3, which is characterized in that the ORAC value of the antioxidant is 1.20
~1.30.
5. application described in any one of -4 according to claim 1, which is characterized in that the antioxidant is anti-oxidation medicine.
6. application according to claim 5, which is characterized in that the anti-oxidation medicine further includes pharmaceutically acceptable auxiliary
Material.
7. application according to claim 6, which is characterized in that the pharmaceutically acceptable auxiliary material includes filler, glues
Mixture, disintegrating agent, lubricant, glidant, hypertensor, effervescent agent, surfactant, film forming agent, toner, corrigent, anti-corrosion
In agent, dispersing agent or aromatic any one or at least two combination.
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|---|---|---|---|---|
| CN111830173A (en) * | 2020-08-04 | 2020-10-27 | 甘肃省药品检验研究院 | Internal standard method for detecting content of wild spina gleditsiae doped in spina gleditsiae decoction pieces |
| CN111830173B (en) * | 2020-08-04 | 2022-10-18 | 甘肃省药品检验研究院 | Internal standard method for detecting content of wild spina gleditsiae doped in spina gleditsiae decoction pieces |
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