CN109362704A - It is a kind of can automatic release hydrogen ions in vitro amputation storage bag - Google Patents

It is a kind of can automatic release hydrogen ions in vitro amputation storage bag Download PDF

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Publication number
CN109362704A
CN109362704A CN201811099804.6A CN201811099804A CN109362704A CN 109362704 A CN109362704 A CN 109362704A CN 201811099804 A CN201811099804 A CN 201811099804A CN 109362704 A CN109362704 A CN 109362704A
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CN
China
Prior art keywords
bag
outer bag
amputation
reactive tank
sealing strip
Prior art date
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Pending
Application number
CN201811099804.6A
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Chinese (zh)
Inventor
杨璟辉
韩澍
王立明
陈瑜
丁如梅
章维
陈子奇
胡显律
赵渊宇
裘年存
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Second Affiliated Hospital Army Medical University
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Second Affiliated Hospital Army Medical University
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Publication date
Application filed by Second Affiliated Hospital Army Medical University filed Critical Second Affiliated Hospital Army Medical University
Priority to CN201811099804.6A priority Critical patent/CN109362704A/en
Publication of CN109362704A publication Critical patent/CN109362704A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths

Abstract

The invention discloses it is a kind of can automatic release hydrogen ions in vitro amputation storage bag, including the first outer bag, the second outer bag, inner bag and reverse osmosis membrane bag;Inner bag forms cold-storage layer between the first outer bag, the second outer bag respectively;Inner bag inner wall note has temperature sensor, and the first outer bag outer wall is equipped with temperature indicator;The bottom of first outer bag is equipped with drainpipe;Reverse osmosis membrane bag includes bag body and sample-adding reactant, and bag body includes porous support layer and compacted zone, and sample-adding reactant includes at calcium hydride sample-adding, the first reactive tank and the second reactive tank.The configuration of the present invention is simple, amputation is saved using bilayer, cold-storage layer realizes the refrigeration to amputation, and the temperature of refrigeration can be monitored in real time, meet the greatest requirements of amputation preservation, will removed water by reverse osmosis membrane and separated with the substance other than hydrogen, guarantee the quality of preservation liquid, and sustained release is achieved the purpose that by being loaded reactant, has extended the release time of hydrogen, improve the survival rate of the replantation of a severed limb.

Description

It is a kind of can automatic release hydrogen ions in vitro amputation storage bag
Technical field
The invention belongs to medical equipment fields, and in particular to it is a kind of can automatic release hydrogen ions in vitro amputation storage bag.
Background technique
With the development of society, various mechanical equipments, communication are rapidly developed and progress, but it is more and more People also has some potential safety problems, such as amputation, severed finger etc..The patient of amputation is relatively common, master in clinical position It to include dialysis caused by cutting dialysis, roll off dialysis, crush amputation, lacerative division and explosive high temperature roller, The indication that there is most of patient acute disease to replant operation.The most important significance that in vitro amputation saves is prepared for the replantation of a severed limb, Beat next good basis.And the condition for thering are some patients not have the replantation of a severed limb, it has to bear due to losing a certain portion Divide physiology and psychological pain brought by limbs, quality of life is caused to decline.These do not have the patient of surgical indication to be mostly Due to multiple injury cause vital sign it is unstable, it is far away can not reach hospital in time, more limb detachments cause operating time compared with Limb necrosis caused by the reasons such as long.In these cases, if it is possible to the limbs for properly saving detachment, when postponing its necrosis Between, replanting for amputation can be carried out again until situation allows.
The 1930s, Allen proposed that drying is just clinically for the preservation of amputation using " dry refrigeration " first It is that directly amputation is not immersed among each class I liquid I, refrigeration is just to maintain low-temperature preservation, and temperature can not be too low, Ying Bao 0 DEG C -4 DEG C are held, this method is used till today always and is recorded in surgery teaching material.But this store method saves amputation Limited time can not be applied to the amputation saved for a long time.In recent years, organ preservative fluid is applied to disconnected by domestic and foreign scholars The preservation of limb, and Related Experimental Study has been carried out, obtain extraordinary as a result, its conserving time limit extends 2 compared with " dry refrigeration " Times or more.In recent years, both at home and abroad some researchers by H2It is added in organ preservative fluid, it is found that this preservation liquid can significantly subtract Light graft because of the damage caused by long-term cooling jet flow, restore by the later period for improving graft.Some researchers answer hydrogen-rich UW liquid For the cryo-conservation of rat kidney, H is found2It is free that the oxygen that can significantly remove and generate during cryo-conservation is added in UW liquid Base generates anti-oxidative stress.Because of H2Molecular weight is small, can permeate into cytoplasm, line grain rapidly through biological membranous structure Body and nucleus, so this scavenging effect is distributed across entire cell.Hydrogen-rich UW liquid is equally used for by certain researchers Rat kidney saves, and the Graft survival rate after discovery preservation 24 hours is between UW liquid group and hydrogen-rich UW liquid group without obvious poor It is different.When reaching 36 hours between when saved, the Graft survival rate that UW liquid saves is only 40%, and the transplanting that hydrogen-rich UW liquid saves Object survival rate has reached 90%, it was demonstrated that hydrogen-rich saves liquid and is woven with better protective effect to the group for needing preservation for a long time.
During scene occurs to hospital by accident, the condition saved to amputation is limited, usually saves amputation It realizes and saves in vessel, realized and refrigerated using mixture of ice and water, but real-time monitoring can not be carried out to the temperature of refrigeration and adjusted Control, not can guarantee the best cold storage effect to amputation, and vessel can not preferably seal amputation, amputation it is easy by External influence is not achieved preferable preservation demand, not can guarantee the longer holding time, surviving after reducing the replantation of a severed limb Rate or function;And amputation now saves in vessel that there is no dedicated for providing the simple machine or dress of hydrogen for liquid storage Set, individually toward addition hydrogen and inconvenient is saved in liquid, hydrogen rich in itself is consumed in saving liquid finish after, also need Toward again toward save liquid in add hydrogen, it is however generally that need certain power, not only consume the energy, waste people's Time, while operation is also more troublesome.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the invention to propose it is a kind of can automatic release hydrogen ions in vitro amputation Storage bag, the storage bag structure is simple, is saved using bilayer to amputation, the organ preservative fluid in inner bag ensure that amputation Preservation effect, the cold-storage layer between inner bag and outer bag realizes the refrigeration to amputation, and can carry out to the temperature of refrigeration real When monitor, meet amputation preservation greatest requirements, and will reverse osmosis membrane bag investment save liquid in can directly for preservation liquid provide Enough hydrogen will be removed water by bag body and be separated with the substance other than hydrogen, will be preferably minimized, and be extended to the influence for saving liquid The release time of hydrogen improves the survival rate of the replantation of a severed limb.
In order to achieve the above purpose, the technical scheme adopted by the invention is that:
It is a kind of can automatic release hydrogen ions in vitro amputation storage bag, including the first outer bag, the second outer bag, an inner bag and Reverse osmosis membrane bag, the first outer bag bottom are connected with the second outer bag lower rotation, top movable connection;The inner bag is located at outside first Inside bag and the second outer bag, inner bag bottom is fixedly connected by the first connector with the first outer bag, the second outer bag inner wall, inner bag Two sides pass through the second connector and are fixedly connected with the first outer bag, the second outer bag inner wall, and posting port, institute are equipped at the top of the inner bag It states posting port edge and is each attached to the first outer bag, the second outer bag inner wall;The inner bag is respectively between the first outer bag, the second outer bag Form the cold-storage layer for being put into cold-storage material;First outer bag and the second outer bag top exterior walls are equipped with the opening for being put into cold-storage material;Institute Stating inner bag inner wall note has temperature sensor, and the first outer bag outer wall is equipped with the temperature indicator being connected with temperature sensor;Outside first The bottom of bag is equipped with the drainpipe with drain valve, and the drainpipe is connected to inner bag;
The reverse osmosis membrane bag is located inside inner bag, and the reverse osmosis membrane bag includes bag body and sample-adding reactant, the bag Body includes porous support layer and compacted zone from inside to outside, the sample-adding reactant from top to bottom successively includes at calcium hydride sample-adding, First reactive tank and the second reactive tank, first reactive tank and the second reactive tank are respectively positioned on inside bag body, and the calcium hydride adds It is connected at the top of bottom end and the first reactive tank at sample, top is equipped with sealing cover and sealing cover stretches out on the outside of bag body;First reaction The bottom of slot is connected to second reactive tank one end, the second reactive tank other end closing;First reactive tank is in inversion circle Mesa-shaped and side wall have been evenly arranged multiple side openings, and second reactive tank is column, U-shaped shape or helical form;The top of the bag body Portion is equipped with draught line, and the end of the draught line is equipped with the drop handle for being fixed on the first outer bag outer wall.
Further, being respectively fixed with the first sealing strip for sealing posting port with the second outer bag at the top of the first outer bag With the second sealing strip;It being set on first sealing strip there are two flexible handle, the flexible handle includes insert and operating body, It is set on first sealing strip there are two the first fixation hole for being passed through for insert, sets that there are two for embedding on second sealing strip Enter the second fixation hole of body insertion, the second fixation hole inner wall is equipped with the card slot for fixedly embedded body;First sealing Inner sidewall is equipped with the wire casing passed through for draught line, is equipped with the hook hung for drop handle on the outside of first outer bag.
Further, the insertion body sidewall is equipped with the card body for being stuck in card slot, the second fixation hole side wall Equipped with the limit hole mobile for card body;When the first sealing strip and the second sealing strip to be fixed, insert is fixed first It is moved in hole, the card body for being embedded in body sidewall moves in the limit hole in the second fixation hole, and rotation operating body drives insert to exist Rotated in first fixation hole and the second fixation hole, while driving rotation of the card body in limit hole, thus allow the first sealing strip and Second sealing strip is fixed together.
Further, the bottom of top insertion the first sealing strip and the second sealing strip of the posting port, the first sealing The outside of the first outer bag and the second outer bag is respectively positioned on the outside of item and the second sealing strip, inside is respectively positioned on the inside of posting port.
Further, the opening of the opening and closing of opening for convenience, the first outer bag and the second outer bag is equipped with zipper.
Further, transport to the storage bag for convenience, set that there are two harness, the harnesses one on the storage bag It is fixedly connected at the top of end and the first sealing strip, the other end is fixedly connected with the second outer bag bottom.
Further, the cold-storage material is blue ice;Lan Bing is commonly called as environmental protective ice, is called cold-storage material, and Lan Bing is a kind of efficient Cold-storage carrier, it is nontoxic, will not burn and explode, it is non-corrosive and reusable.
Further, the storage bag is when in use, organ preservative fluid is housed in the inner bag;The organ preservative fluid master Will be there are also lactobionic acid, main is impermeability anion, and molecular weight is relatively large, and cell occurs swollen when can reduce refrigeration The problems such as swollen, significantly extends the holding time of amputation and the activity of cell.
Further, first connector and the second connector are elastic fabric.
Further, the aperture of the porous support layer is 0.5nm~2nm, thickness is greater than 100um, the densification Layer with a thickness of 20~nm, the aperture of the side opening is 0.5nm~2nm.
The beneficial effects of the present invention are:
Storage bag structure proposed by the invention is simple, by two layers of the first outer bag, the second outer bag and inner bag to amputation into Row saves;It holds the organ preservative fluid for saving amputation in inner bag to save amputation, at the top of two outer bags First sealing strip and the second sealing strip can to internal bag in amputation and organ preservative fluid be sealed, avoid to the storage bag into When row transport, unstable occur amputation because transporting or the case where organ preservative fluid is poured out, greatly reduce the shadow of outer bound pair amputation It rings, strengthens the preservation effect to amputation, and the movable fixation between the first sealing strip and the second sealing strip can facilitate to disconnected Limb is taken, very convenient, and the first outer bag and the second outer bag are sufficiently stable when linking together, and is reduced because transport bring is shaken It is dynamic that amputation is affected;Cold-storage layer between inner bag and two outer bags for placing cold-storage material, by cold-storage material to internal bag in Amputation refrigerated, closed between inner bag and two outer bags, guarantee preferable refrigerated enclosure space;Pass through temperature simultaneously Sensor is measured in real time the temperature of cold-storage layer, and by temperature display detected on temperature indicator, medical worker can Suitable cold-storage material is added into cold-storage layer according to the temperature information shown on temperature indicator, guarantees that amputation is in best refrigeration In range, meet the best Conservation environment of amputation, extend holding time and the cell changes of amputation, after improving the replantation of a severed limb Survival rate and function;When being replaced to organ preservative fluid, facilitated by the drainpipe of outer bag bottom by internal organ Liquid discharge is saved, avoids affecting amputation when replacement saves liquid;
The reverse osmosis membrane bag for discharging hydrogen is equipped in inner bag in the present invention, reverse osmosis membrane bag is suspended on preservation In the inner bag of liquid, the inside for the sample-adding reactant that water enters in hair permeable membrane bag reacts with calcium hydride, generates hydrogen, hydrogen Enter through side opening and reverse osmosis membrane bag and save in liquid, achievees the effect that hydrogen-rich;By the bag body in reverse osmosis membrane and have side All other substance isolation other than water removal and gas is got up, avoids calcium hydride and calcium hydroxide saturating by first reactive tank in hole It crosses bag body and is impacted to liquid is saved, so that saving liquid in the case where being rich in hydrogen, itself will not be still contaminated, and guarantee to save The effect of liquid;It can reach the purpose of hydrogen sustained-release by being loaded reactant, when putting into water, water initially enters reverse osmosis membrane bag In first reactive tank, calcium hydride and water fast reaction in the first reactive tank discharge a large amount of hydrogen, then only need maintaining liquid The concentration of hydrogen in body, water gradually invade in the second reactive tank after end of reaction in the first reactive tank, instead due to second It answers in slot, due in long strip in the second reactive tank, the reacting dose of calcium hydride and water is lower and the hydrogen that discharges in the unit time It is less, while reaching sustained release purpose, maintain the concentration of hydrogen molecule in liquid.
Detailed description of the invention
Fig. 1 is complete structure schematic diagram of the invention;
Fig. 2 is the side view when present invention closes;
Fig. 3 is the side view when present invention opens;
Fig. 4 is front section view of the invention;
Fig. 5 is the structural schematic diagram that the first sealing strip and the second sealing strip are fixed;
Fig. 6 is the decomposition diagram of the first sealing strip and the second sealing strip;
Fig. 7 is the schematic diagram of the first sealing strip;
Fig. 8 is the schematic diagram of the second sealing strip;
Fig. 9 is the schematic diagram of flexible handle;
Figure 10 is the structural schematic diagram of reverse osmosis membrane bag;
Figure 11 is the structural schematic diagram for being loaded reactant.
In figure: 1, the first outer bag;1-1, drainpipe;1-2, hook;2, the second outer bag;3, inner bag;4, the first sealing strip;4- 1, the first fixation hole;4-2, wire casing;5, the second sealing strip;5-1, the second fixation hole;5-2, limit hole;5-3, card slot;6, movable Handle;6-1, insert;6-2, card body;7, it is open;7-1, zipper;8, harness;9, the first connector;10, the second connector; 11, temperature indicator;12, compacted zone;13, porous support layer;14, at calcium hydride sample-adding;14-1, sealing cover;15, the first reaction Slot;15-1, side opening;16, the second reactive tank;17, draught line;17-1, drop handle.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made into one with reference to the accompanying drawing Step illustrates.
In the present embodiment, as shown in figs. 1-11, it is a kind of can automatic release hydrogen ions in vitro amputation storage bag, including the One outer bag 1, the second outer bag 2, inner bags 3 and a reverse osmosis membrane bags, 1 bottom of the first outer bag are connected with 2 lower rotation of the second outer bag, Top movable connection;The inner bag 3 is located inside the first outer bag 1 and the second outer bag 2,3 bottom of inner bag by the first connector 9 with First outer bag 1,2 inner wall of the second outer bag are fixedly connected, and the two sides of inner bag 3 pass through the second connector 10 and the first outer bag 1, second 2 inner wall of outer bag is fixedly connected, and first connector 9 and the second connector 10 are elastic fabric;It is equipped at the top of the inner bag 3 Posting port, the posting port edge are each attached to the first outer bag 1,2 inner wall of the second outer bag;The inner bag 3 respectively with the first outer bag 1, the cold-storage layer for being put into cold-storage material is formed between the second outer bag 2, the cold-storage material is blue ice;Lan Bing is commonly called as environmental protective ice, is called Cold-storage material, Lan Bing is a kind of efficient cold-storage carrier, nontoxic, will not burn and explode, non-corrosive and reusable; First outer bag 1 and 2 top exterior walls of the second outer bag are equipped with the opening 7 for being put into cold-storage material, are equipped with zipper 7-1 at opening 7;Institute Stating 3 inner wall note of inner bag has temperature sensor, and 1 outer wall of the first outer bag is equipped with the temperature indicator 11 being connected with temperature sensor;The The bottom of one outer bag 1 is equipped with drainpipe 1-1, and the drainpipe 1-1 is connected to inner bag 3, is equipped with rubber in the end of drainpipe 1-1 Plug;
The reverse osmosis membrane bag is located inside inner bag 3, and the reverse osmosis membrane bag includes bag body and sample-adding reactant, the bag Body includes porous support layer 13 and compacted zone 12 from inside to outside, and the sample-adding reactant from top to bottom successively includes that calcium hydride is loaded The 14, first reactive tank 15 of place and the second reactive tank 16, first reactive tank 15 and the second reactive tank 16 are respectively positioned on inside bag body, It is connected at the top of 14 bottom ends and the first reactive tank 15 at the calcium hydride sample-adding, top is equipped with sealing cover 14-1 and sealing cover 14-1 stretches The external side of output;The bottom of first reactive tank 15 is connected to 16 one end of the second reactive tank, and second reactive tank 16 is another End seal is closed;First reactive tank 15 has been evenly arranged multiple side opening 15-1, second reaction in inverted round table shape and side wall Slot 16 is in long strip;The top of the bag body is equipped with draught line 17, and the end of the draught line 17, which is equipped with, is fixed on the first outer bag 1 The drop handle 1-2 of outer wall;The aperture of the porous support layer 13 is 0.5nm~2nm, and thickness is greater than 100um, the compacted zone 12 With a thickness of 20~nm, the aperture of the side opening 15-1 is 0.5nm~2nm;Second reactive tank 16 be column, U-shaped shape or Helical form;
The first sealing strip 4 and second that first outer bag, 1 top and the second outer bag 2 are respectively fixed with for sealing posting port is close Strip of paper used for sealing 5, the bottom of top insertion the first sealing strip 4 and the second sealing strip 5 of the posting port, the first sealing strip 4 and second are close The outside of strip of paper used for sealing 5 is respectively positioned on the outside of the first outer bag 1 and the second outer bag 2, and inside is respectively positioned on the inside of posting port;Described first is close It is set on strip of paper used for sealing 4 there are two flexible handle 6, the flexible handle 6 includes insert 6-1 and operating body, on first sealing strip 4 If setting that there are two embedding for insert 6-1 on second sealing strip 5 there are two the first fixation hole 4-1 for passing through for insert 6-1 The the second fixation hole 5-1 entered, the second fixation hole 5-1 inner wall are equipped with the card slot 5-3 for fixedly embedded body 6-1;It is described embedding Enter body 6-1 side wall equipped with the card body 6-2 for being stuck in card slot 5-3, the first fixation hole 4-1 side wall is equipped with for card body 6-2 Mobile limit hole 5-2;When the first sealing strip 4 and the second sealing strip 5 to be fixed, insert 6-1 is in the first fixation hole It is moved in 5-1, the card body 6-2 of insert 6-1 side wall is moved in limit hole 5-2, and rotation operating body drives insert 6-1 the It is rotated in one fixation hole 4-1 and the second fixation hole 5-1, while card body 6-2 being driven to turn in card slot 5-3, to allow first close Strip of paper used for sealing 4 and the second sealing strip 5 are fixed together;First sealing strip, 4 inner sidewall is equipped with the wire casing 4- passed through for draught line 17 2, the hook 1-2 hung for drop handle 17-1 is equipped on the outside of first outer bag 1.
Transport to the storage bag for convenience, sets that there are two harness 8, described 8 one end of harness and first on the storage bag It is fixedly connected at the top of sealing strip 4, the other end is fixedly connected with 2 bottom of the second outer bag.
The storage bag when in use, is equipped with organ preservative fluid in the inner bag 2;Mainly there are also lactose for the organ preservative fluid The problems such as acid, main is impermeability anion, and molecular weight is relatively large, the swelling that cell occurs when can reduce refrigeration, Significantly extend the holding time of amputation and the activity of cell.
When the raw accidents such as amputation or severed finger of human hair, wound is handled and is wrapped up first, to prevent big bleeding, then Amputation is handled, avoids bringing secondary damage to amputation;Two flexible handles 6 are rotated, the first sealing strip 4 and second is close Strip of paper used for sealing 5 separates, and opens the posting port at 3 top of inner bag, suitable organ preservative fluid is filled in inner bag 3, is put in organ preservative fluid Enter reverse osmosis membrane bag, bag body and sample reactant, which are respectively positioned on, to be saved in liquid, and then amputation is placed in inner bag 3, is protected by organ Liquid storage is saved, and the first sealing strip 4 and the second sealing strip 5 are aligned, by rotating flexible handle 6 for 4 He of the first sealing strip Second sealing strip 5 is fixed, and the draught line 17 of reverse osmosis membrane bag passes through wire casing 4-2, and the drop handle 17-1 of 17 end of draught line is hung It realizes and fixes at hook 1-2;Then open two opening 7, respectively toward between the first outer bag 1 and inner bag 3,2 and of the second outer bag Lan Bing is put between inner bag 3, at this time the temperature sensor knot that environment locating for amputation is checked, and will test in 3 to internal bag Fruit is shown on temperature indicator 11, and medical worker is controlled by quantity of the temperature shown by temperature indicator 1 to blue ice System guarantees that amputation is in the temperature range most preferably refrigerated, meets the best Conservation environment of amputation, extend the holding time of amputation And cell changes, survival rate and function after improving the replantation of a severed limb;After preservation finishes, directly by the first sealing strip 4 It is opened with the second sealing strip 5;It, will be interior by drainpipe 11 when needing to replace organ preservative fluid during preservation Portion's organ preservative fluid is expelled directly out, and reduces the influence to amputation, new organ preservative fluid is then directly added by posting port;
When using reverse osmosis membrane bag, it is first turned on sealing cover 14-1, the plus hydrogenated calcium into sample-adding reactant, until first Calcium hydride is each filled in reactive tank 15 and the second reactive tank 16;Then the reverse osmosis membrane bag is thrown in organ preservative fluid, then Draught line 17 is passed through into wire casing 4-2, the drop handle 17-1 of 17 end of draught line is hung on the hook 1-2 outside the first outer bag 1;
After reverse osmosis membrane bag is put in a liquid, water through bag body enter in reverse osmosis membrane bag sample-adding reactant inside with Calcium hydride reacts, and generates hydrogen, and hydrogen enters through side opening 15-1 and reverse osmosis membrane to be saved in liquid, reaches the effect of hydrogen-rich Fruit;By the bag body in reverse osmosis membrane and the first reactive tank 15 with side opening 15-1, by other than water removal and gas it is all its The isolation of its substance is got up, and calcium hydride and calcium hydroxide is avoided to impact through the reverse osmosis membrane bag to liquid, so that saving liquid In the case where being rich in hydrogen, remains to not be contaminated, guarantee the function of itself script;Sample-adding reactant has reached hydrogen sustained-release Purpose, when reverse osmosis membrane bag is put into water, water is initially entered in the first reactive tank 15, the calcium hydride in the first reactive tank 15 With water fast reaction, a large amount of hydrogen are discharged;With the investment of reverse osmosis membrane bag, the concentration of hydrogen in maintaining liquid body is only needed, Water is gradually immersed into the second reactive tank 16 in the first reactive tank after 15 end of reaction, since the second reactive tank 16 is in the form of a column, U The hydrogen that the reacting dose of type shape or helical form, calcium hydride and water is lower and discharges in the unit time is less, calcium hydride slow release Hydrogen achievees the purpose that sustained release to maintain the concentration of hydrogen molecule in liquid;It is directly logical after calcium to be hydrogenated is exhausted Draught line 17 is crossed by the reverse osmosis membrane bag pull-up, then adds calcium hydride or replacement reverse osmosis membrane bag.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its Equivalent thereof.

Claims (9)

1. one kind can automatic release hydrogen ions in vitro amputation storage bag, it is characterised in that: outside including the first outer bag (1), second Bag (2), an inner bag (3) and reverse osmosis membrane bag, the first outer bag (1) bottom are connected with the second outer bag (2) lower rotation, and top is living Dynamic connection;The inner bag (3) is located at the first outer bag (1) and the second outer bag (2) is internal, and inner bag (2) bottom passes through the first connector (9) be fixedly connected with the first outer bag (1), the second outer bag (2) inner wall, the two sides of inner bag (3) pass through the second connector (10) with First outer bag (1), the second outer bag (2) inner wall are fixedly connected, and posting port, the posting port edge are equipped at the top of the inner bag (3) It is each attached to the first outer bag (1), the second outer bag (2) inner wall;The inner bag (3) respectively with the first outer bag (1), the second outer bag (2) Between formed for being put into the cold-storage layer of cold-storage material;First outer bag (1) and the second outer bag (2) top exterior walls are equipped with and are put into cold-storage material Opening (7);Inner bag (3) the inner wall note has a temperature sensor, and the first outer bag (1) outer wall is equipped with to be connected with temperature sensor Temperature indicator (11);The bottom of first outer bag (1) is equipped with drainpipe (1-1), and the drainpipe (1-1) is connected to inner bag (3);
The reverse osmosis membrane bag is located inside inner bag, the reverse osmosis membrane bag include bag body and sample-adding reactant, the bag body by It is interior and outside include porous support layer (13) and compacted zone (12), the sample-adding reactant from top to bottom successively including calcium hydride sample-adding Locate (14), the first reactive tank (15) and the second reactive tank (16), first reactive tank (15) and the second reactive tank (16) equal position Inside bag body, it is connected at the top of (14) bottom end and the first reactive tank (15) at the calcium hydride sample-adding, top is equipped with sealing cover (14-1) and sealing cover (14-1) stretch out on the outside of bag body;The bottom and the second reactive tank (16) one end of first reactive tank (15) Connection, the second reactive tank (16) other end closing;First reactive tank (15) is in inverted round table shape and the uniform cloth of side wall It is equipped with multiple side openings (15-1), second reactive tank (16) is column, U-shaped shape or helical form;The top of the bag body is equipped with Draught line (17), the end of the draught line (17) are equipped with the drop handle for being fixed on the first outer bag (1) outer wall.
2. in vitro amputation storage bag according to claim 1, it is characterised in that: the first outer bag (1) top and the second outer bag (2) the first sealing strip (4) and the second sealing strip (5) for sealing posting port are respectively fixed with;On first sealing strip (4) If there are two flexible handle (6), the flexible handle (6) includes insert (6-1) and operating body, first sealing strip (4) On set there are two the first fixation hole (4-1) for being passed through for insert (6-1), set that there are two for embedding on second sealing strip (5) Enter the second fixation hole (5-1) of body (6-1) insertion, the second fixation hole (5-1) inner wall, which is equipped with, is used for fixedly embedded body (6-1) Card slot (5-3);First sealing strip (4) inner sidewall is equipped with the wire casing (4-2) that passes through for draught line (17), outside described first The hook (1-2) hung for drop handle (17-1) is equipped on the outside of bag (1).
3. in vitro amputation storage bag according to claim 2, it is characterised in that: insert (6-1) side wall, which is equipped with, to be used In the card body (6-2) being stuck in card slot (5-3), the second fixation hole (5-1) side wall is equipped with the limit mobile for card body (6-2) Hole (5-2).
4. in vitro amputation storage bag according to claim 3, it is characterised in that: the top insertion first of the posting port is close The first outer bag is respectively positioned on the outside of the bottom of strip of paper used for sealing (4) and the second sealing strip (5), the first sealing strip (4) and the second sealing strip (5) (1) and the outside of the second outer bag (2), inside are respectively positioned on the inside of posting port.
5. in vitro amputation storage bag according to claim 4, it is characterised in that: the first outer bag (1) and the second outer bag (2) Zipper (8) are equipped at opening (7).
6. in vitro amputation storage bag according to claim 5, it is characterised in that: it is set on the storage bag there are two harness (8), It is fixedly connected at the top of described harness (8) one end and the first sealing strip (4), the other end is fixedly connected with the second outer bag (2) bottom.
7. in vitro amputation storage bag according to claim 1, it is characterised in that: the cold-storage material is blue ice.
8. in vitro amputation storage bag according to claim 1, it is characterised in that: first connector (9) and second connects Junctor (10) is elastic fabric.
9. in vitro amputation storage bag according to claim 1, it is characterised in that: the aperture of the porous support layer (13) is 0.5nm~2nm, thickness be greater than 100um, the compacted zone (12) with a thickness of 20~nm, the aperture of the side opening (15-1) For 0.5nm~2nm.
CN201811099804.6A 2018-09-20 2018-09-20 It is a kind of can automatic release hydrogen ions in vitro amputation storage bag Pending CN109362704A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040148857A1 (en) * 2003-02-05 2004-08-05 Michael Strizki Hydrogen gas generation system
CN103523389A (en) * 2012-07-06 2014-01-22 株式会社光未来 Dissolution method of hydrogen and hydrogen in a sealed bag
WO2014021416A1 (en) * 2012-08-01 2014-02-06 株式会社光未来 Hydrogen filling container employing hydrogen-filled sealed pouch, and hydrogen filling cap employing hydrogen-filled sealed pouch
CN105246492A (en) * 2014-03-13 2016-01-13 水株式会社 Method for producing hydrogen-containing biological application solution, and exterior body therefor
CN204969117U (en) * 2015-05-19 2016-01-20 王敏 First -aid dressing is preserved to severed finger

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040148857A1 (en) * 2003-02-05 2004-08-05 Michael Strizki Hydrogen gas generation system
CN103523389A (en) * 2012-07-06 2014-01-22 株式会社光未来 Dissolution method of hydrogen and hydrogen in a sealed bag
WO2014021416A1 (en) * 2012-08-01 2014-02-06 株式会社光未来 Hydrogen filling container employing hydrogen-filled sealed pouch, and hydrogen filling cap employing hydrogen-filled sealed pouch
CN105246492A (en) * 2014-03-13 2016-01-13 水株式会社 Method for producing hydrogen-containing biological application solution, and exterior body therefor
CN204969117U (en) * 2015-05-19 2016-01-20 王敏 First -aid dressing is preserved to severed finger

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