CN109355264A - A kind of wheat ACCase mutated genes and its application in plant antiweed - Google Patents

Abstract

The invention discloses a kind of wheat ACCase mutated genes and its application in plant antiweed, there are any one or a few following mutation for the amino acid sequence of ACCase mutated genes coding albumen: it mutates corresponding to the 1708th of amino acid sequence of wild-type wheat ACCase, the 1768th mutate, the 1769th mutate, the 1992nd mutate, the 2015th mutate, the 2029th mutate, the 2066th mutate, the 2084th mutation.Plant, which expresses the mutain, can resist acetyl-CoA carboxylase inhibitor class herbicide.The ACCase mutated genes can be widely applied to plant antiweed breeding.

Description

A kind of wheat ACCase mutated genes and its application in plant antiweed

Technical field

The present invention relates to a kind of wheat ACCase mutated genes and its applications in plant antiweed, belong to plant The amino acid sequence in genetic engineering and plant antiweed breeding field, the albumen of the coded by said gene corresponds to wild-type wheat There are any one or more following mutation for the amino acid sequence of ACCase: the amino acid sequence corresponding to wild-type wheat ACCase The 1708th of column mutates, the 1768th mutate, the 1769th mutate, the 1992nd mutate, the 2015 mutations, the 2029th mutation, the 2066th mutation, the 2084th mutation.

Background technique

Acetyl-CoA carboxylase (ACCase) class inhibitor is the herbicide of ultra high efficiency, is had to gramineae weed excellent Preventive effect, and it is highly safe to dicotyledon, thus it is most commonly used in the big Tanaka of broad leaf crop at present.But it finds simultaneously Such herbicide of long-term single use, dosage generally can all increase year by year, and drug effect reduces, that is to say, that sensibility weeds are big Amount is reduced, and the tolerant weeds of non-sensibility are gradually increasing and are evolved into dominant species in Schima superba, and the production of easily induced mbc weeds It is raw.

The same acetolactate synthestase of ACCase (ALS) is the target enzymes of highy potent herbicide as polyphenol oxidase (PPO), Mainly there are fragrant phenoxy base phenoxy propionic acid (AOPP) and cyclohexenone analog (CHD) herbicide for the herbicide of the target, they are right Dicotyledon has the selectivity of height, and has low mammalian toxicity and good environmental characteristics, by increasingly More concerns.AOPP class herbicide is a kind of famous high activity herbicide for preventing and kill off gramineae weed, and a kind of development ratio Relatively rapidly, the herbicide type for issuing new varieties is not turned off.Such herbicide was begun one's study in the 1960s earliest.Mesh The AOPP class herbicide of preceding commercialization has diclofop-methyl, surely kills, haloxyfop-r-methyl, dislikes careless ether etc..Such herbicide exists three-dimensional Isomery, and only have d-isomer to have remarkable activity to ACCase in this isomers.After being handled with cover grass energy, plant fat oxygen Synthase activity increases, and unsaturated fat acid oxidase aggravation, free radical amplifies Damage to film；Apply various external source lipoxygenase suppressions The toxic action of diclofop-methyl and cover grass energy can be reversed in preparation and hydroxyl radical scavenger, and forms diclofop-methyl induction ethylene Effect is inhibited.

Due to being widely used for chemical herbicide, the speed that Weed Resistance occurs is so fast, range is so wide, not only Weeding cost is increased, environmental pressure is also increased.Currently, the reason of antagonism weed population formation, there are two types of viewpoints: a kind of Think that weeds contact with herbicide for a long time, and the encoding gene mutation at herbicide action position or gene expression occurs Change, as a result causes weeds that the kind that there is drug resistance or drug resistance to herbicide is improved or occurred to the detoxification ability of herbicide Group.Generally speaking, the process that the formation of Weed Resistance, substantially a resistant gene are selected, the heredity of drug resistance gene It is the basis that weeds develop drug resistance.Report before thinks that the generation of drug resistance is not only due to plant changes to weeding The absorption of agent accelerates the metabolism of herbicide with transhipment or plant, and the site of action for being primarily due to herbicide is changed Become.Currently, still lacking the ACCase mutated genes that can be efficiently used, limiting the initiative of herbicide resistant plants material and being somebody's turn to do The efficient utilization of class herbicide.

Summary of the invention

Technical problem: gramineae plant is highly sensitive to ACCase inhibitor class herbicide, skill to be solved by this invention Art problem is to provide a kind of mutated genes and mutein that can assign Genes For Plant Tolerance Herbicid resistant.Further, originally The technical problems to be solved by the invention, which are to provide one kind, can assign Genes For Plant Tolerance (resistance to) acetyl-CoA carboxylase class Herbicid resistant Wheat ACCase mutated genes and mutein.

Technical solution: ACCase genic mutation type is obtained by the method for rite-directed mutagenesis, utilizes the method for molecular biology The ACCase mutated genes are cloned, then the method for the gene genetic transformation is imported to the recipient plant to herbicide sensitive Genome expresses ACCase mutein, and recipient plant is made to have the resistance to ACCase inhibitor class herbicide.It is described There is following mutation in the amino acid sequence that ACCase mutated genes encode albumen: its ammonia for corresponding to wild-type wheat ACCase The 1708th mutation, the 1768th mutation, the 1769th mutation, the 1992nd generation of base acid sequence are prominent Become, the 2015th mutation, the 2029th mutation, the 2066th mutation, the 2084th mutation.Wild type The 1708th of the amino acid sequence of wheat ACCase is arginine originally, can be any amino in addition to arginine after mutation Acid；1768th is asparagine originally, can be the arbitrary amino acid in addition to asparagine after mutation；1769th was different bright originally Propylhomoserin can be the arbitrary amino acid in addition to isoleucine after mutation；1992nd is alanine originally, can be for except the third ammonia after mutation Arbitrary amino acid except acid；2015th is tryptophan originally, can be the arbitrary amino acid in addition to tryptophan after mutation；The 2029 are isoleucine originally, can be the arbitrary amino acid in addition to isoleucine after mutation；2066th is aspartic acid originally, It can be the arbitrary amino acid in addition to aspartic acid after mutation；2084th is glycine originally, after mutation can for except glycine it Outer arbitrary amino acid.

The albumen of the wheat ACCase mutated genes expression, comprising:

(a) its amino acid sequence be selected from by SEQ ID NO.2, SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8, In the group that SEQ ID NO.10, SEQ ID NO.12, SEQ ID NO.14, SEQ ID NO.16 are formed；Or

(b) amino acid sequence in (a) is by replacing and/or being deleted and/or added one or more amino acid, and has There is the protein as derived from (a) of activity of acetyl coenzyme A carboxylase.

The present invention also provides a kind of nucleic acid or gene, the coding albumen.

Nucleic acid or gene of the present invention, comprising:

(i) nucleic acid or gene of the coding albumen；Or

(ii) under strict conditions with (i) nucleotide sequence hybridization limited and coding has acetyl-CoA carboxylase living The nucleotide sequence of the protein of property；Or

(iii) its nucleotide sequence is selected from by SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO: 7, in the group of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15 composition.

The stringent condition are as follows: 0.1 × SSC, 0.1%SDS solution in, 65 DEG C to wash 15 minutes height of film rigorous Hybridization conditions.

The nucleotide that ACCase mutated genes of the invention mutate includes but are not limited to following several mutation, Following site mutation at the case where other nucleotide also within protection scope of the present invention, such as: A sports T, C, G；Or C Sport T, A, G；Or T sports A, C, G；Or to sport T, C, A etc. within the scope of the present invention by G.

Further, wheat ACCase mutated genes according to the present invention, there are following for the wheat ACCase gene One or more mutation: it only mutates, in the 5122nd nucleotide of wheat ACCase gene only in wheat ACCase gene The 5303rd nucleotide mutate, only wheat ACCase gene the 5309th nucleotide mutate, only in wheat 5975th nucleotide of ACCase gene mutates, is only prominent in the 6045th nucleotide generation of wheat ACCase gene Become, only in the 6085th nucleotide mutation of wheat ACCase gene, only in the 6197th core of wheat ACCase gene Thuja acid mutates, only in the 6250th nucleotide mutation of wheat ACCase gene or multiple sites, generation is prominent simultaneously Become, such as the 5122nd nucleotide and the 5303rd nucleotide mutate simultaneously, the 5122nd nucleotide, the 5303rd nucleosides Acid and the 5309th nucleotide mutates simultaneously, the 5122nd nucleotide, the 5303rd nucleotide, the 5309th nucleotide and The mutation etc. simultaneously of 5975th nucleotide.

Preferably, wheat ACCase mutated genes according to the present invention, there are following for the wheat ACCase gene One or more mutation: only T is sported by C, only in wheat ACCase base in the 5122nd nucleotide of wheat ACCase gene 5303rd nucleotide of cause sports G by A, sports T by A, small in the 5309th nucleotide of wheat ACCase gene 5975th nucleotide of wheat ACCase gene sports T by C, is dashed forward in the 6045th nucleotide of wheat ACCase gene by G Become T, A sported by T, in the 6197th core of wheat ACCase gene in the 6085th nucleotide of wheat ACCase gene Thuja acid sports G by A, is sported A or multiple sites in the 6250th nucleotide of wheat ACCase gene by G while being occurred Mutation.

The present invention also provides a kind of expression cassette, recombinant vector or cell, contain the ACCase mutated genes.

The herbicide is one of diclofop-methyl and cover grass energy or a variety of.

The present invention also provides the method with the plant of Herbicid resistant is obtained, include the following steps:

1) making plant includes the nucleic acid or gene；Or

2) plant is made to express the wheat ACCase mutein.

Further, the method includes crispr gene editing, transgenosis, hybridization, backcrossing or vegetative propagation steps.

The present invention also provides a kind of methods of plant identification, wherein the plant is the plant comprising the nucleic acid or gene The plant of albumen described in object, expression or the plant obtained by the method, comprising the following steps:

1) measure whether the plant includes the nucleic acid or gene；Or,

2) measure whether the plant expresses the albumen.

The present invention also provides a kind of protective agent of herbicide, which includes the wheat ACCase saltant type egg It is white.

The present invention also provides a kind of methods for controlling weeds, comprising: applies removing for effective dose to the crop field of long-term cropping Careless agent, the crop include the nucleic acid or gene or the expression cassette, recombinant vector or cell, and the herbicide is standing grain One of careless spirit and cover grass energy are a variety of.

The present invention also provides a kind of methods for protecting the plants from the damage as caused by herbicide, comprising: to plantation The herbicide of the crop field application effective dose of crop, the crop include the nucleic acid or gene or by the expression cassette, Recombinant vector imports plant, and the plant after importing generates herbicide resistance proteins, and the herbicide is in diclofop-methyl and cover grass energy It is one or more.

The utility model has the advantages that the ACCase mutated genes and its application method disclosed by the invention from wheat can assign Herbicide sensitive plant to the resistance of herbicide takes this that herbicide resistant plants can be cultivated, to realize that crops crop field is efficient Chemical weed control.The wheat ACCase mutated genes can also be by the traditional breeding way such as hybridizing, being returned to common wheat Kind transfer, cultivates antiweed new variety of wheat.

Detailed description of the invention

Fig. 1 shows the schematic diagram of the transgenic wheat antiweed containing the mutational site ACCase.

Fig. 2 shows the ACCase full length gene result figures that PCR amplification contains the mutational site wheat ACCase；1st swimming lane For Marker；Marker molecular weight be followed successively by from top to bottom 15kb, 8kb, 5kb, 3kb, 2kb, 1.5kb, 1kb, 750bp, 500bp, the 2nd swimming lane are the DNA of wild type Xu wheat No. 33, and the 3rd swimming lane is to contain antiweed mutational site ACCase-1708's DNA, the 4th swimming lane are the DNA containing antiweed mutational site ACCase-1768, and the 5th swimming lane is to be mutated position containing antiweed The DNA of point ACCase-1769, the 6th swimming lane be the DNA containing antiweed mutational site ACCase-1992, the 7th swimming lane be containing There is the DNA of antiweed mutational site ACCase-2015, the 8th swimming lane is to contain antiweed mutational site ACCase-2029's DNA, the 9th swimming lane are the DNA containing antiweed mutational site ACCase-2066, and the 10th swimming lane is to be mutated containing antiweed The DNA of site ACCase-2084.

Fig. 3 shows saltant type ACCase gene transgenic wheat PCR detection figure；A total of 8 swimming lanes, the 1st swimming lane are Marker；Marker molecular weight is followed successively by 15kb, 8kb, 5kb, 3kb, 2kb, 1.5kb, 1kb, 750bp, 500bp from top to bottom； 2nd swimming lane is non-transgenic wild type DNA, and as negative control, the 3rd swimming lane is Plasmid DNA, as positive control, the 4th swimming lane To convert the transgenic plant DNA containing ACCase-1708 antiweed mutant ACCase gene, the 5th swimming lane is that conversion contains There is the transgenic plant DNA of ACCase-1768 antiweed mutant ACCase gene, the 6th swimming lane is that conversion contains ACCase- The transgenic plant DNA of 1769 antiweed mutant ACCase genes, the 7th swimming lane are to convert to resist containing ACCase-1992 to remove The transgenic plant DNA of careless agent mutant ACCase gene, the 8th swimming lane are that conversion is mutated containing ACCase-2015 antiweed The transgenic plant DNA of body ACCase gene, the 9th swimming lane are that conversion contains ACCase-2029 antiweed mutant ACCase The transgenic plant DNA of gene, the 10th swimming lane are that conversion turns containing ACCase-2066 antiweed mutant ACCase gene Gene plant DNA, the 11st swimming lane are transgenic plant of the conversion containing ACCase-2084 antiweed mutant ACCase gene DNA。

Specific embodiment

Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.It is not specified in embodiment specific Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is It can be with conventional products that are commercially available.

Embodiment 1: the determination of wheat ACCase gene mutation site

We regard the ACCase sequence (Genbank XM_015783727.2) of wheat as reference sequences, with wheat cdna Group carries out homologous comparison, the homology highest of discovery and the ACCase gene (AF029895.1) of Wheat volatiles.According to patent The rice ACCase mentioned in (US15715547, CN201811176178.6) can with the amino acid mutation site of antiweed, It is 1731 (arginine sports cysteine) respectively, 1791 (asparagine mutation is serine), 1792 (isoleucine becomes Leucine), 2015 (alanine becomes valine), 2038 (tryptophan becomes cysteine), 2052 (isoleucine becomes asparagus fern Amide), 2089 (aspartic acid becomes glycine), 2107 (glycine becomes serine) as reference, pass through homologous comparison Method, determine in wheat the amino acid sites of ACCase gene mutation be respectively 1708 (arginine sports cysteine), 1768 (asparagine mutation is serine), 1769 (isoleucine becomes leucine), 1992 (alanine becomes valine), 2015 (tryptophan becomes cysteine), 2029 (isoleucine becomes asparagine), 2066 (aspartic acid becomes glycine), 2084 (glycine becomes serine).According to the amino acid codes table of comparisons, determine that corresponding nucleotide site is respectively 5122 (C Become T), 5303 (A becomes G), 5309 (A becomes T), 5975 (C becomes T), 6045 (G becomes T), 6085 (T becomes A), 6197 (A becomes G), 6250 (G becomes A).

Embodiment 2: the acquisition of No. 33 wild type ACCase full-length genes of wheat Xu wheat

The blade of No. 33 WT lines of Xu wheat is chosen, genomic DNA is extracted, according to wheat ACCase gene in NCBI (NCBI Genbank:AF029895) design primer is expanded, and (is purchased from using KOD DNA polymerase polymerase Toyobo company) ACCase gene is expanded, reaction system is as follows:

Sterile water is added to 50 μ l of total volume

Pcr amplification reaction program uses two-step method, and annealing and extension are combined into together, using 68 degree.

Program is as follows: initial denaturation: 94 DEG C of 3min；35 circulations: 94 DEG C of 10sec of denaturation；Extend 68 DEG C of 10min；Heat preservation: 72 ℃10min。

2 μ l PCR products are taken to detect through 1% agarose gel electrophoresis, discovery has after the segment of expected size (Fig. 2), remaining PCR product after PCR cleaning agents box (be purchased from Axygen company) cleaning recycling, be cloned into pMD19-T carrier and (be purchased from Takara company), then convert Escherichia coli.Each random 12 Escherichia coli clones of picking of conversion carry out PCR detection, take 6 monoclonals that PCR result is positive send Nanjing one of Biotechnology Co., Ltd's sequencing, obtain mutation ACCase gene sequence Column, the genetic fragment length are 13127bp, by obtaining full length gene 6936bp, No. 33 wild types of Xu wheat after removal introne The sequencing result of ACCase sequence genetic fragment is as shown in SEQ ID No:17.

Embodiment 3: the acquisition of wheat ACCase gene mutation site gene order

Utilize the mutational site sequence of point mutation kit amplification wheat ACCase gene.Firstly, utilizing primer ACC-CF And ACC-CR clones ACCase full-length cDNA, is connected into TK007 carrier and (holds up biology section of section in Nanjing using No. 33 cDNA of Xu wheat as template Skill Co., Ltd), Escherichia coli TOP10 bacterial strain competent cell is converted, positive transformant sequence verification is selected, is obtained correct single Clone, plasmid are named as TK007-ACCase；Second, using mutant primer ACC-MutF and ACC-MutR, with TK007- ACCase is template, carries out PCR amplification and is transferred to Escherichia coli DMT bacterial strain sense after amplified production restriction enzyme DpnI digestion By state cell, positive transformant sequence verification is selected, obtains the plasmid of ACCase target site mutation.

The mutational site ACCase-1708, nucleosides are expanded using mutant primer ACC1708-MutF and ACC1708-MutR The 5122nd of acid sequence sports T by C, and the 1708th of the amino acid sequence for causing it to encode becomes half Guang ammonia from arginine Acid, nucleotide sequence is as shown in SEQ ID No:1, and the amino acid sequence of coding is as shown in SEQ ID No:2；

The mutational site ACCase-1768, nucleosides are expanded using mutant primer ACC1768-MutF and ACC1768-MutR The 5303rd of acid sequence sports G by A, and the 1768th of the amino acid sequence for causing it to encode becomes ammonia from asparagine Acid, nucleotide sequence is as shown in SEQ ID No:3, and the amino acid sequence of coding is as shown in SEQ ID No:4；

The mutational site ACCase-1769, nucleosides are expanded using mutant primer ACC1769-MutF and ACC1769-MutR The 5309th of acid sequence sports T by A, and the 1769th of the amino acid sequence for causing it to encode becomes bright ammonia from isoleucine Acid, nucleotide sequence is as shown in SEQ ID No:5, and the amino acid sequence of coding is as shown in SEQ ID No:6；

The mutational site ACCase-1992, nucleosides are expanded using mutant primer ACC1992-MutF and ACC1992-MutR The 5975th of acid sequence sports T by C, and the 1992nd of the amino acid sequence for causing it to encode becomes figured silk fabrics ammonia from alanine Acid, nucleotide sequence is as shown in SEQ ID No:7, and the amino acid sequence of coding is as shown in SEQ ID No:8；

The mutational site ACCase-2015, nucleosides are expanded using mutant primer ACC2015-MutF and ACC2015-MutR Acid sequence the 6045th sports T by G, and the 2010th of the amino acid sequence for causing it to encode becomes half Guang ammonia from tryptophan Acid, nucleotide sequence is as shown in SEQ ID No:9, and the amino acid sequence of coding is as shown in SEQ ID No:10.

The mutational site ACCase-2029, nucleosides are expanded using mutant primer ACC2029-MutF and ACC2029-MutR The 6085th of acid sequence sports A by T, and the 2029th of the amino acid sequence for causing it to encode becomes asparagus fern from isoleucine Amide, nucleotide sequence is as shown in SEQ ID No:11, and the amino acid sequence of coding is as shown in SEQ ID No:12.

The mutational site ACCase-2066, nucleosides are expanded using mutant primer ACC2066-MutF and ACC2066-MutR The 6197th of acid sequence sports G by A, causes amino acid sequence the 2066th that it is encoded to become sweet ammonia from aspartic acid Acid, nucleotide sequence is as shown in SEQ ID No:13, and the amino acid sequence of coding is as shown in SEQ ID No:14.

The mutational site ACCase2084, nucleotide are expanded using mutant primer ACC2084-MutF and ACC2084-MutR Sequence the 6250th sports A by G, causes amino acid sequence the 2084th that it is encoded to become alanine, core from glycine Nucleotide sequence is as shown in SEQ ID No:15, and the amino acid sequence of coding is as shown in SEQ ID No:16.

The acquisition of embodiment 4ACCase transgenic wheat and its antiweed verifying

Reacted by Infuison, the ACCase segment of mutation is connected into containing cauliflower mosaic virus 35 S promoter and In the plant expression vector of 35S terminator, wild-type wheat is converted respectively.Homozygous and stable expression is obtained to be mutated containing ACCase The transgenic wheat in site, and to the tolerance of transgenic wheat detection herbicide.

Transgenic wheat and wild-type wheat are sowed respectively, when wheat length to tri-leaf period, spray acetyl coenzyme A respectively Carboxylase inhibitor class herbicide quizalofop-ethyl (spraying concentration is application 1.5mL quizalofop-ethyl/L water (2 times are recommended to use concentration)), pyrrole Fluorine chlorine standing grain spirit (spraying concentration is application 1.5mL haloxyfop/L water (1 times is recommended to use concentration)), clethodim (spraying concentration For apply 1.5mL clethodim/L water (1 times is recommended to use concentration)), fluazifop (spraying concentration be apply 1.5mL pyrrole fluorine standing grain Careless spirit/L water (1 times is recommended to use concentration)), investigate withered situation after spraying one month, discovery wild type control spray and with Above after the acetyl-CoA carboxylase inhibitor class herbicide quizalofop-ethyl, clethodim, fluazifop of same concentration, almost stop Growth, newly longer blade is completely withered and yellow and gradually withered.And the transgenic wheat containing the mutational site ACCase is then whole Anti- phenotype is shown to acetyl-CoA carboxylase inhibitor class herbicide, normal growth and development is almost without by shadow It rings, final normal jointing heading.Show transgenic wheat of the present invention to the resistance of ACCase inhibition herbicide than wild in table 1 Type wants high, and injury percentage wants low 60% or more.This shows that the transgenosis for carrying the mutational site ACCase that we obtain is small Wheat can actually stablize the resistance of the anti-acetyl-CoA carboxylase inhibitor class herbicide of acquisition of heredity.

Injury rate of the 1 acetyl-CoA carboxylase class herbicide of table to wild type and transgenic wheat

Although a specific embodiment of the invention has been described in detail, it will be understood to those of skill in the art that according to All introductions having disclosed can be carry out various modifications and be replaced to those details, these are in protection scope of the present invention It is interior.Full scope of the invention is provided by extremely any equivalent of appended patent requirements.

Sequence table

<110>Jiangsu Province Agriculture Science Institute

<120>make plant that there is ACCase mutein and its application of Herbicid resistant

<160> 36

<170> SIPOSequenceListing 1.0

<210> 1

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1708 mutant nucleotide sequence)

<400> 1

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ctgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 2

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1708 amino acid sequence variants)

<400> 2

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Cys Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 3

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1768 mutant nucleotide sequence)

<400> 3

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gagcatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 4

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1768 amino acid sequence variants)

<400> 4

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Ser Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 5

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1769 mutant nucleotide sequence)

<400> 5

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatactt ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 6

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1769 amino acid sequence variants)

<400> 6

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Leu His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 7

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1992 mutant nucleotide sequence)

<400> 7

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagttacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 8

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-1992 amino acid sequence variants)

<400> 8

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Val Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 9

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2015 mutant nucleotide sequence)

<400> 9

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actgtagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 10

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2015 amino acid sequence variants)

<400> 10

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Cys Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 11

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2029 mutant nucleotide sequence)

<400> 11

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaaacctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 12

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2029 amino acid sequence variants)

<400> 12

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Asn Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 13

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2066 mutant nucleotide sequence)

<400> 13

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattggtag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 14

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2066 amino acid sequence variants)

<400> 14

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Gly Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 15

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2084 mutant nucleotide sequence)

<400> 15

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaaga gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 16

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase-2084 amino acid sequence variants)

<400> 16

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Ser Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 17

<211> 6936

<212> DNA

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase wild-type nucleotide sequences)

<400> 17

atgggatcca cacatttgcc cattgtcggc cttaatgcct cgacaacacc atcgctatcc 60

actattcgcc cggtaaattc agccggtgct gcattccaac catctgcccc ttctagaacc 120

tccaagaaga aaagtcgtcg tgttcagtca ttaagggatg gaggcgatgg aggcgtgtca 180

gaccctaacc agtctattcg ccaaggtctt gccggcatca ttgacctccc aaaggagggc 240

acatcagctc cggaagtgga tatttcacat gggtccgaag aacccagggg ctcctaccaa 300

atgaatggga tactgaatga agcacataat gggaggcatg cttcgctgtc taaggttgtc 360

gaattttgta tggcattggg cggcaaaaca ccaattcaca gtgtattagt tgcgaacaat 420

ggaatggcag cagctaagtt catgcggagt gtccgaacat gggctaatga aacatttggg 480

tcagagaagg caattcagtt gatagctatg gctactccag aagacatgag gataaatgca 540

gagcacatta gaattgctga tcaatttgtt gaagtacccg gtggaacaaa caataacaac 600

tatgcaaatg tccaactcat agtggagata gcagtgagaa ccggtgtttc tgctgtttgg 660

cctggttggg gccatgcatc tgagaatcct gaacttccag atgcactaaa tgcaaacgga 720

attgtttttc ttgggccacc atcatcatca atgaacgcac taggtgacaa ggttggttca 780

gctctcattg ctcaagcagc aggggttccg actcttcctt ggagtggatc acaggtggaa 840

attccattag aagtttgttt ggactcgata cccgcggaga tgtataggaa agcttgtgtt 900

agtactacgg aggaagcact tgcgagttgt cagatgattg ggtatcccgc catgattaaa 960

gcatcatggg gtggtggtgg taaagggatc cgaaaggtta ataatgacga tgatgtcaga 1020

gcactgttta agcaagtgca aggtgaagtt cctggctccc caatatttat catgagactt 1080

gcatctcaga gtcgacatct tgaagttcag ttgctttgtg atcaatatgg caatgtagct 1140

gcgcttcaca gtcgtgactg cagtgtgcaa cggcgacacc aaaagattat tgaggaagga 1200

ccagttactg ttgctcctcg cgagacagtg aaagagctag agcaagcagc aaggaggctt 1260

gctaaggctg tgggttatgt tggtgctgct actgttgaat atctctacag catggagact 1320

ggtgaatact attttctgga acttaatcca cggttgcagg ttgagcatcc agtcaccgag 1380

tggatagctg aagtaaactt gcctgcagct caagttgcag ttggaatggg tatacccctt 1440

tggcaggttc cagagatcag acgtttctat ggaatggaca atggaggagg ctatgacatt 1500

tggaggaaaa cagcagctct tgctactcca tttaacttcg atgaagtgga ttctcaatgg 1560

ccaaagggtc attgtgtagc agttaggata accagtgagg atccagatga cggattcaag 1620

cctaccggtg gaaaagtaaa ggagatcagt tttaaaagca agccaaatgt ttgggcctat 1680

ttctctgtta agtccggtgg aggcattcat gaatttgctg attctcagtt tggacatgtt 1740

tttgcatatg gagtgtctag agcagcagca ataaccaaca tgtctcttgc gctaaaagag 1800

attcaaattc gtggagaaat tcattcaaat gttgattaca cagttgatct cttgaatgcc 1860

tcagacttca aagaaaacag gattcatact ggctggctgg ataacagaat agcaatgcga 1920

gtccaagctg agagacctcc gtggtatatt tcagtggttg gaggagctct atataaaaca 1980

ataacgagca acacagacac tgtttctgaa tatgttagct atctcgtcaa gggtcagatt 2040

ccaccgaagc atatatccct tgtccattca actgtttctt tgaatataga ggaaagcaaa 2100

tatacaattg aaactataag gagcggacag ggtagctaca gattgcgaat gaatggatca 2160

gttattgaag caaatgtcca aacattatgt gatggtggac ttttaatgca gttggatgga 2220

aacagccatg taatttatgc tgaagaagag gccggtggta cacggcttct aattgatgga 2280

aagacatgct tgttacagaa tgatcacgat ccttcaaggt tattagctga gacaccctgc 2340

aaacttcttc gtttcttggt tgccgatggt gctcatgttg aagctgatgt accatatgcg 2400

gaagttgagg ttatgaagat gtgcatgccc ctcttgtcac ctgctgctgg tgtcattaat 2460

gttttgttgt ctgagggcca gcctatgcag gctggtgatc ttatagcaag acttgatctt 2520

gatgaccctt ctgctgtgaa gagagctgag ccatttaacg gatctttccc agaaatgagc 2580

cttcctattg ctgcttctgg ccaagttcac aaaagatgtg ccacaagctt gaatgctgct 2640

cggatggtcc ttgcaggata tgatcacccg atcaacaaag ttgtacaaga tctggtatcc 2700

tgtctagatg ctcctgagct tcctttccta caatgggaag agcttatgtc tgttttagca 2760

actagacttc caaggcttct taagagcgag ttggagggta aatacagtga atataagtta 2820

aatgttggcc atgggaagag caaggatttc ccttccaaga tgctaagaga gataatcgag 2880

gaaaatcttg cacatggttc tgagaaggaa attgctacaa atgagaggct tgttgagcct 2940

cttatgagcc tactgaagtc atatgagggt ggcagagaaa gccatgcaca ctttattgtg 3000

aagtcccttt tcgaggacta tctctcggtt gaggaactat tcagtgatgg cattcagtct 3060

gatgtgattg aacgcctgcg ccaacaacat agtaaagatc tccagaaggt tgtagacatt 3120

gtgttgtctc accagggtgt gagaaacaaa actaagctga tactaacact catggagaaa 3180

ctggtctatc caaaccctgc tgtctacaag gatcagttga ctcgcttttc ctccctcaat 3240

cacaaaagat attataagtt ggcccttaaa gctagcgagc ttcttgaaca aaccaagctt 3300

agtgagctcc gcacaagcat tgcaaggagc ctttcagaac ttgagatgtt tactgaagaa 3360

aggacggcca ttagtgagat catgggagat ttagtgactg ccccactgcc agttgaagat 3420

gcactggttt ctttgtttga ttgtagtgat caaactcttc agcagagggt gatcgagacg 3480

tacatatctc gattatacca gcctcatctt gtcaaggata gtatccagct gaaatatcag 3540

gaatctggtg ttattgcttt atgggaattc gctgaagcgc attcagagaa gagattgggt 3600

gctatggtta ttgtgaagtc gttagaatct gtatcagcag caattggagc tgcactaaag 3660

ggtacatcac gctatgcaag ctctgagggt aacataatgc atattgcttt attgggtgct 3720

gataatcaaa tgcatggaac tgaagacagt ggtgataacg atcaagctca agtcaggata 3780

gacaaacttt ctgcgacact ggaacaaaat actgtcacag ctgatctccg tgctgctggt 3840

gtgaaggtta ttagttgcat tgttcaaagg gatggagcac tcatgcctat gcgccatacc 3900

ttcctcttgt cggatgaaaa gctttgttat gaggaagagc cggttctccg gcatgtggag 3960

cctcctcttt ctgctcttct tgagttgggt aagttgaaag tgaaaggata caatgaggtg 4020

aagtatacac cgtcacgtga tcgtcagtgg aacatataca cacttagaaa tacagagaac 4080

cccaaaatgt tgcacagggt gtttttccga actcttgtca ggcaacccgg tgcttccaac 4140

aaattcacat caggcaacat cagtgatgtt gaagtgggag gagctgagga atctctttca 4200

tttacatcga gcagcatatt aagatcgctg atgactgcta tagaagagtt ggagcttcac 4260

gcgattagga caggtcactc tcatatgttt ttgtgcatat tgaaagagca aaagcttctt 4320

gatcttgttc ccgtttcagg gaacaaagtt gtggatattg gccaagatga agctactgca 4380

tgcttgcttc tgaaagaaat ggctctacag atacatgaac ttgtgggtgc aaggatgcat 4440

catctttctg tatgccaatg ggaggtgaaa cttaagttgg acagcgatgg gcctgccagt 4500

ggtacctgga gagttgtaac aaccaatgtt actagtcaca cctgcactgt ggatatctac 4560

cgtgaggtcg aagatacaga atcacagaaa ctagtgtacc actctgctcc atcgtcatct 4620

ggtcctttgc atggcgttgc actgaatact ccatatcagc ctttgagtgt tattgatctg 4680

aaacgttgct ccgctagaaa taacagaact acatactgct atgattttcc gttggcattt 4740

gaaactgcag tgcagaagtc atggtctaac atttctagtg acactaaccg atgttatgtt 4800

aaagcgacgg agctggtgtt tgctcacaag aacgggtcat ggggcactcc tgtaattcct 4860

atggagcgtc ctgctgggct caatgacatt ggtatggtag cttggatctt ggacatgtcc 4920

actcctgaat atcccaatgg caggcagatt gttgtcatcg caaatgatat tacttttaga 4980

gctggatcgt ttggtccaag ggaagatgca ttttttgaaa ctgttaccaa cctagcttgt 5040

gagaggaagc ttcctctcat ctacttggca gcaaactctg gtgctcggat cggcatagca 5100

gatgaagtaa aatcttgctt ccgtgttgga tggtctgatg atggcagccc tgaacgtggg 5160

tttcaatata tttatctgac tgaagaagac catgctcgta ttagcgcttc tgttatagcg 5220

cacaagatgc agcttgataa tggtgaaatt aggtgggtta ttgattctgt tgtagggaag 5280

gaggatgggc taggtgtgga gaacatacat ggaagtgctg ctattgccag tgcctattct 5340

agggcctatg aggagacatt tacgcttaca tttgtgactg gaaggactgt tggaatagga 5400

gcatatcttg ctcgacttgg catacggtgc atacagcgta ctgaccagcc cattatccta 5460

actgggttct ctgccttgaa caagcttctt ggccgggaag tttacagctc ccacatgcag 5520

ttgggtggcc ccaaaattat ggcgacaaac ggtgttgtcc atctgacagt ttcagatgac 5580

cttgaaggtg tatctaatat attgaggtgg ctcagctatg ttcctgccaa cattggtgga 5640

cctcttccta ttacaaaatc tttggaccca cctgacagac ccgttgctta catccctgag 5700

aatacatgcg atcctcgtgc tgccatcagt ggcattgatg atagccaagg gaaatggttg 5760

gggggcatgt tcgacaaaga cagttttgtg gagacatttg aaggatgggc gaagtcagtt 5820

gttactggca gagcgaaact cggagggatt ccggtgggtg ttatagctgt ggagacacag 5880

actatgatgc agctcatccc tgctgatcca ggccagcttg attcccatga gcgatctgtt 5940

cctcgtgctg ggcaagtctg gtttccagat tcagctacta agacagcgca ggcaatgctg 6000

gacttcaacc gtgaaggatt acctctgttc atccttgcta actggagagg cttctctggt 6060

ggacaaagag atctttttga aggaatcctt caggctgggt caacaattgt tgagaacctt 6120

aggacataca atcagcctgc ctttgtatat atccccaagg ctgcagagct acgtggaggg 6180

gcttgggtcg tgattgatag caagataaat ccagatcgca ttgagttcta tgctgagagg 6240

actgcaaagg gcaatgttct cgaacctcaa gggttgatcg agatcaagtt caggtcagag 6300

gaactccaag agtgcatggg taggcttgat ccagaattga taaatctgaa ggcaaagctc 6360

cagggagtaa agcatgaaaa tggaagtcta cctgagtcag aatcccttca gaagagcata 6420

gaagcccgga agaaacagtt gttgcctttg tatactcaaa ttgcggtacg gttcgctgaa 6480

ttgcatgaca cttcccttag aatggctgct aagggtgtga ttaagaaggt tgtagactgg 6540

gaagattcta ggtcgttctt ctacaagaga ttacggagga ggatatccga ggatgttctt 6600

gcgaaggaaa ttagaggtgt aagtggcaag cagttttctc accaatcggc aatcgagctg 6660

atccagaaat ggtacttggc ctctaaggga gctgaaacag gaagcactga atgggatgat 6720

gacgatgctt ttgttgcctg gagggaaaac cctgaaaact accaggagta tatcaaagaa 6780

ctcagggctc aaagggtatc tcagttgctc tcagatgttg cagactccag tccagatcta 6840

gaagccttgc cacagggtct ttctatgcta ttagagaaga tggatccctc aaggagagca 6900

cagtttgttg aggaagtcaa gaaagtcctt aaatga 6936

<210> 18

<211> 2311

<212> PRT

<213>Xu wheat No. 33 (Triticum aestivum L.ACCase wild-type amino acid sequence)

<400> 18

Met Gly Ser Thr His Leu Pro Ile Val Gly Leu Asn Ala Ser Thr Thr

1 5 10 15

Pro Ser Leu Ser Thr Ile Arg Pro Val Asn Ser Ala Gly Ala Ala Phe

20 25 30

Gln Pro Ser Ala Pro Ser Arg Thr Ser Lys Lys Lys Ser Arg Arg Val

35 40 45

Gln Ser Leu Arg Asp Gly Gly Asp Gly Gly Val Ser Asp Pro Asn Gln

50 55 60

Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Lys Glu Gly

65 70 75 80

Thr Ser Ala Pro Glu Val Asp Ile Ser His Gly Ser Glu Glu Pro Arg

85 90 95

Gly Ser Tyr Gln Met Asn Gly Ile Leu Asn Glu Ala His Asn Gly Arg

100 105 110

His Ala Ser Leu Ser Lys Val Val Glu Phe Cys Met Ala Leu Gly Gly

115 120 125

Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly Met Ala Ala

130 135 140

Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Glu Thr Phe Gly

145 150 155 160

Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro Glu Asp Met

165 170 175

Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe Val Glu Val

180 185 190

Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln Leu Ile Val

195 200 205

Glu Ile Ala Val Arg Thr Gly Val Ser Ala Val Trp Pro Gly Trp Gly

210 215 220

His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Asn Ala Asn Gly

225 230 235 240

Ile Val Phe Leu Gly Pro Pro Ser Ser Ser Met Asn Ala Leu Gly Asp

245 250 255

Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val Pro Thr Leu

260 265 270

Pro Trp Ser Gly Ser Gln Val Glu Ile Pro Leu Glu Val Cys Leu Asp

275 280 285

Ser Ile Pro Ala Glu Met Tyr Arg Lys Ala Cys Val Ser Thr Thr Glu

290 295 300

Glu Ala Leu Ala Ser Cys Gln Met Ile Gly Tyr Pro Ala Met Ile Lys

305 310 315 320

Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val Asn Asn Asp

325 330 335

Asp Asp Val Arg Ala Leu Phe Lys Gln Val Gln Gly Glu Val Pro Gly

340 345 350

Ser Pro Ile Phe Ile Met Arg Leu Ala Ser Gln Ser Arg His Leu Glu

355 360 365

Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala Leu His Ser

370 375 380

Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile Glu Glu Gly

385 390 395 400

Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu Glu Gln Ala

405 410 415

Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala Ala Thr Val

420 425 430

Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe Leu Glu Leu

435 440 445

Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp Ile Ala Glu

450 455 460

Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly Ile Pro Leu

465 470 475 480

Trp Gln Val Pro Glu Ile Arg Arg Phe Tyr Gly Met Asp Asn Gly Gly

485 490 495

Gly Tyr Asp Ile Trp Arg Lys Thr Ala Ala Leu Ala Thr Pro Phe Asn

500 505 510

Phe Asp Glu Val Asp Ser Gln Trp Pro Lys Gly His Cys Val Ala Val

515 520 525

Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro Thr Gly Gly

530 535 540

Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val Trp Ala Tyr

545 550 555 560

Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala Asp Ser Gln

565 570 575

Phe Gly His Val Phe Ala Tyr Gly Val Ser Arg Ala Ala Ala Ile Thr

580 585 590

Asn Met Ser Leu Ala Leu Lys Glu Ile Gln Ile Arg Gly Glu Ile His

595 600 605

Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser Asp Phe Lys

610 615 620

Glu Asn Arg Ile His Thr Gly Trp Leu Asp Asn Arg Ile Ala Met Arg

625 630 635 640

Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val Gly Gly Ala

645 650 655

Leu Tyr Lys Thr Ile Thr Ser Asn Thr Asp Thr Val Ser Glu Tyr Val

660 665 670

Ser Tyr Leu Val Lys Gly Gln Ile Pro Pro Lys His Ile Ser Leu Val

675 680 685

His Ser Thr Val Ser Leu Asn Ile Glu Glu Ser Lys Tyr Thr Ile Glu

690 695 700

Thr Ile Arg Ser Gly Gln Gly Ser Tyr Arg Leu Arg Met Asn Gly Ser

705 710 715 720

Val Ile Glu Ala Asn Val Gln Thr Leu Cys Asp Gly Gly Leu Leu Met

725 730 735

Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu Glu Ala Gly

740 745 750

Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Leu Leu Gln Asn Asp

755 760 765

His Asp Pro Ser Arg Leu Leu Ala Glu Thr Pro Cys Lys Leu Leu Arg

770 775 780

Phe Leu Val Ala Asp Gly Ala His Val Glu Ala Asp Val Pro Tyr Ala

785 790 795 800

Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser Pro Ala Ala

805 810 815

Gly Val Ile Asn Val Leu Leu Ser Glu Gly Gln Pro Met Gln Ala Gly

820 825 830

Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala Val Lys Arg

835 840 845

Ala Glu Pro Phe Asn Gly Ser Phe Pro Glu Met Ser Leu Pro Ile Ala

850 855 860

Ala Ser Gly Gln Val His Lys Arg Cys Ala Thr Ser Leu Asn Ala Ala

865 870 875 880

Arg Met Val Leu Ala Gly Tyr Asp His Pro Ile Asn Lys Val Val Gln

885 890 895

Asp Leu Val Ser Cys Leu Asp Ala Pro Glu Leu Pro Phe Leu Gln Trp

900 905 910

Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg Leu Leu Lys

915 920 925

Ser Glu Leu Glu Gly Lys Tyr Ser Glu Tyr Lys Leu Asn Val Gly His

930 935 940

Gly Lys Ser Lys Asp Phe Pro Ser Lys Met Leu Arg Glu Ile Ile Glu

945 950 955 960

Glu Asn Leu Ala His Gly Ser Glu Lys Glu Ile Ala Thr Asn Glu Arg

965 970 975

Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu Gly Gly Arg

980 985 990

Glu Ser His Ala His Phe Ile Val Lys Ser Leu Phe Glu Asp Tyr Leu

995 1000 1005

Ser Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp Val Ile Glu

1010 1015 1020

Arg Leu Arg Gln Gln His Ser Lys Asp Leu Gln Lys Val Val Asp Ile

1025 1030 1035 1040

Val Leu Ser His Gln Gly Val Arg Asn Lys Thr Lys Leu Ile Leu Thr

1045 1050 1055

Leu Met Glu Lys Leu Val Tyr Pro Asn Pro Ala Val Tyr Lys Asp Gln

1060 1065 1070

Leu Thr Arg Phe Ser Ser Leu Asn His Lys Arg Tyr Tyr Lys Leu Ala

1075 1080 1085

Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser Glu Leu Arg

1090 1095 1100

Thr Ser Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe Thr Glu Glu

1105 1110 1115 1120

Arg Thr Ala Ile Ser Glu Ile Met Gly Asp Leu Val Thr Ala Pro Leu

1125 1130 1135

Pro Val Glu Asp Ala Leu Val Ser Leu Phe Asp Cys Ser Asp Gln Thr

1140 1145 1150

Leu Gln Gln Arg Val Ile Glu Thr Tyr Ile Ser Arg Leu Tyr Gln Pro

1155 1160 1165

His Leu Val Lys Asp Ser Ile Gln Leu Lys Tyr Gln Glu Ser Gly Val

1170 1175 1180

Ile Ala Leu Trp Glu Phe Ala Glu Ala His Ser Glu Lys Arg Leu Gly

1185 1190 1195 1200

Ala Met Val Ile Val Lys Ser Leu Glu Ser Val Ser Ala Ala Ile Gly

1205 1210 1215

Ala Ala Leu Lys Gly Thr Ser Arg Tyr Ala Ser Ser Glu Gly Asn Ile

1220 1225 1230

Met His Ile Ala Leu Leu Gly Ala Asp Asn Gln Met His Gly Thr Glu

1235 1240 1245

Asp Ser Gly Asp Asn Asp Gln Ala Gln Val Arg Ile Asp Lys Leu Ser

1250 1255 1260

Ala Thr Leu Glu Gln Asn Thr Val Thr Ala Asp Leu Arg Ala Ala Gly

1265 1270 1275 1280

Val Lys Val Ile Ser Cys Ile Val Gln Arg Asp Gly Ala Leu Met Pro

1285 1290 1295

Met Arg His Thr Phe Leu Leu Ser Asp Glu Lys Leu Cys Tyr Glu Glu

1300 1305 1310

Glu Pro Val Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu Glu

1315 1320 1325

Leu Gly Lys Leu Lys Val Lys Gly Tyr Asn Glu Val Lys Tyr Thr Pro

1330 1335 1340

Ser Arg Asp Arg Gln Trp Asn Ile Tyr Thr Leu Arg Asn Thr Glu Asn

1345 1350 1355 1360

Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln Pro

1365 1370 1375

Gly Ala Ser Asn Lys Phe Thr Ser Gly Asn Ile Ser Asp Val Glu Val

1380 1385 1390

Gly Gly Ala Glu Glu Ser Leu Ser Phe Thr Ser Ser Ser Ile Leu Arg

1395 1400 1405

Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg Thr

1410 1415 1420

Gly His Ser His Met Phe Leu Cys Ile Leu Lys Glu Gln Lys Leu Leu

1425 1430 1435 1440

Asp Leu Val Pro Val Ser Gly Asn Lys Val Val Asp Ile Gly Gln Asp

1445 1450 1455

Glu Ala Thr Ala Cys Leu Leu Leu Lys Glu Met Ala Leu Gln Ile His

1460 1465 1470

Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp Glu

1475 1480 1485

Val Lys Leu Lys Leu Asp Ser Asp Gly Pro Ala Ser Gly Thr Trp Arg

1490 1495 1500

Val Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile Tyr

1505 1510 1515 1520

Arg Glu Val Glu Asp Thr Glu Ser Gln Lys Leu Val Tyr His Ser Ala

1525 1530 1535

Pro Ser Ser Ser Gly Pro Leu His Gly Val Ala Leu Asn Thr Pro Tyr

1540 1545 1550

Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn

1555 1560 1565

Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val

1570 1575 1580

Gln Lys Ser Trp Ser Asn Ile Ser Ser Asp Thr Asn Arg Cys Tyr Val

1585 1590 1595 1600

Lys Ala Thr Glu Leu Val Phe Ala His Lys Asn Gly Ser Trp Gly Thr

1605 1610 1615

Pro Val Ile Pro Met Glu Arg Pro Ala Gly Leu Asn Asp Ile Gly Met

1620 1625 1630

Val Ala Trp Ile Leu Asp Met Ser Thr Pro Glu Tyr Pro Asn Gly Arg

1635 1640 1645

Gln Ile Val Val Ile Ala Asn Asp Ile Thr Phe Arg Ala Gly Ser Phe

1650 1655 1660

Gly Pro Arg Glu Asp Ala Phe Phe Glu Thr Val Thr Asn Leu Ala Cys

1665 1670 1675 1680

Glu Arg Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly Ala Arg

1685 1690 1695

Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly Trp Ser

1700 1705 1710

Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu Thr Glu

1715 1720 1725

Glu Asp His Ala Arg Ile Ser Ala Ser Val Ile Ala His Lys Met Gln

1730 1735 1740

Leu Asp Asn Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val Gly Lys

1745 1750 1755 1760

Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala Ile Ala

1765 1770 1775

Ser Ala Tyr Ser Arg Ala Tyr Glu Glu Thr Phe Thr Leu Thr Phe Val

1780 1785 1790

Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu Gly Ile

1795 1800 1805

Arg Cys Ile Gln Arg Thr Asp Gln Pro Ile Ile Leu Thr Gly Phe Ser

1810 1815 1820

Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His Met Gln

1825 1830 1835 1840

Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His Leu Thr

1845 1850 1855

Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp Leu Ser

1860 1865 1870

Tyr Val Pro Ala Asn Ile Gly Gly Pro Leu Pro Ile Thr Lys Ser Leu

1875 1880 1885

Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Thr Cys Asp

1890 1895 1900

Pro Arg Ala Ala Ile Ser Gly Ile Asp Asp Ser Gln Gly Lys Trp Leu

1905 1910 1915 1920

Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu Gly Trp

1925 1930 1935

Ala Lys Ser Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile Pro Val

1940 1945 1950

Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Leu Ile Pro Ala

1955 1960 1965

Asp Pro Gly Gln Leu Asp Ser His Glu Arg Ser Val Pro Arg Ala Gly

1970 1975 1980

Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala Met Leu

1985 1990 1995 2000

Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn Trp Arg

2005 2010 2015

Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu Gln Ala

2020 2025 2030

Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro Ala Phe

2035 2040 2045

Val Tyr Ile Pro Lys Ala Ala Glu Leu Arg Gly Gly Ala Trp Val Val

2050 2055 2060

Ile Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Phe Tyr Ala Glu Arg

2065 2070 2075 2080

Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu Ile Lys

2085 2090 2095

Phe Arg Ser Glu Glu Leu Gln Glu Cys Met Gly Arg Leu Asp Pro Glu

2100 2105 2110

Leu Ile Asn Leu Lys Ala Lys Leu Gln Gly Val Lys His Glu Asn Gly

2115 2120 2125

Ser Leu Pro Glu Ser Glu Ser Leu Gln Lys Ser Ile Glu Ala Arg Lys

2130 2135 2140

Lys Gln Leu Leu Pro Leu Tyr Thr Gln Ile Ala Val Arg Phe Ala Glu

2145 2150 2155 2160

Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys

2165 2170 2175

Val Val Asp Trp Glu Asp Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg

2180 2185 2190

Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Gly Val Ser

2195 2200 2205

Gly Lys Gln Phe Ser His Gln Ser Ala Ile Glu Leu Ile Gln Lys Trp

2210 2215 2220

Tyr Leu Ala Ser Lys Gly Ala Glu Thr Gly Ser Thr Glu Trp Asp Asp

2225 2230 2235 2240

Asp Asp Ala Phe Val Ala Trp Arg Glu Asn Pro Glu Asn Tyr Gln Glu

2245 2250 2255

Tyr Ile Lys Glu Leu Arg Ala Gln Arg Val Ser Gln Leu Leu Ser Asp

2260 2265 2270

Val Ala Asp Ser Ser Pro Asp Leu Glu Ala Leu Pro Gln Gly Leu Ser

2275 2280 2285

Met Leu Leu Glu Lys Met Asp Pro Ser Arg Arg Ala Gln Phe Val Glu

2290 2295 2300

Glu Val Lys Lys Val Leu Lys

2305 2310

<210> 19

<211> 25

<212> DNA

<213>ACCase gene wild-type sequence ACC-CF primer (Artificial Sequence)

<400> 19

atgacatcca cacatgtggc gacat 25

<210> 20

<211> 25

<212> DNA

<213>ACCase gene wild-type sequence ACC-CR primer (Artificial Sequence)

<400> 20

tcaaccaagg accttcctga tttct 25

<210> 21

<211> 20

<212> DNA

<213>mutational site ACCase-1708 ACC1708-MutF primer (Artificial Sequence)

<400> 21

ttgcttctgt gttggatggt 20

<210> 22

<211> 20

<212> DNA

<213>mutational site ACCase-1708 ACC1708-MutR primer (Artificial Sequence)

<400> 22

ccaacacaga agcaagattt 20

<210> 23

<211> 20

<212> DNA

<213>mutational site ACCase-1768 ACC1768-MutF primer (Artificial Sequence)

<400> 23

gtggagagca tacatggaag 20

<210> 24

<211> 20

<212> DNA

<213>mutational site ACCase-1768 ACC1768-MutR primer (Artificial Sequence)

<400> 24

atgtatgctc tccacaccta 20

<210> 25

<211> 20

<212> DNA

<213>mutational site ACCase-1769 ACC1769-MutF primer (Artificial Sequence)

<400> 25

aacatacttg gaagtgctgc 20

<210> 26

<211> 20

<212> DNA

<213>mutational site ACCase-1769 ACC1769-MutR primer (Artificial Sequence)

<400> 26

acttccaagt atgttctcca 20

<210> 27

<211> 20

<212> DNA

<213>mutational site ACCase-1992 ACC1992-MutF primer (Artificial Sequence)

<400> 27

gattcagtta ctaagacagc 20

<210> 28

<211> 20

<212> DNA

<213>mutational site ACCase-1992 ACC1992-MutR primer (Artificial Sequence)

<400> 28

cttagtaact gaatctggaa 20

<210> 29

<211> 20

<212> DNA

<213>mutational site ACCase-2015 ACC1992-MutF primer (Artificial Sequence)

<400> 29

ctaactgtag aggcttctct 20

<210> 30

<211> 20

<212> DNA

<213>mutational site ACCase-2015 ACC2015-MutR primer (Artificial Sequence)

<400> 30

agcctctaca gttagcaagg 20

<210> 31

<211> 20

<212> DNA

<213>mutational site ACCase-2029 ACC2029-MutF primer (Artificial Sequence)

<400> 31

tgaaggaaac cttcaggctg 20

<210> 32

<211> 20

<212> DNA

<213>mutational site ACCase-2029 ACC2029-MutR primer (Artificial Sequence)

<400> 32

tgaaggattc cttcaaaaag 20

<210> 33

<211> 20

<212> DNA

<213>mutational site ACCase-2066 ACC2066-MutF primer (Artificial Sequence)

<400> 33

gtgattggta gcaagataaa 20

<210> 34

<211> 20

<212> DNA

<213>mutational site ACCase-2066 ACC2066-MutR primer (Artificial Sequence)

<400> 34

cttgctacca atcacgaccc 20

<210> 35

<211> 20

<212> DNA

<213>mutational site ACCase-2084 ACC2084-MutF primer (Artificial Sequence)

<400> 35

tgcaaagagc aatgttctcg 20

<210> 36

<211> 20

<212> DNA

<213>mutational site ACCase-2084 ACC2084-MutR primer (Artificial Sequence)

<400> 36

acattgctct ttgcagtcct 20

Claims (10)

1. a kind of wheat ACCase mutein, which is characterized in that there are following any one for the amino acid sequence of the albumen Kind or various mutations: the 1708th mutation of the amino acid sequence corresponding to wild-type wheat ACCase, the 1768th hair Raw mutation, the 1769th mutate, the 1992nd mutate, the 2015th mutate, the 2029th mutate, the 2066 mutations, the 2084th mutation.

2. ACCase mutein according to claim 1, which is characterized in that its amino acid sequence such as SEQ ID NO. 2、SEQ ID NO. 4、SEQ ID NO. 6、SEQ ID NO. 8、SEQ ID NO. 10、SEQ ID NO. 12、SEQ ID NO. 14, shown in SEQ ID NO. 16.

3. ACCase mutein according to claim 2, which is characterized in that its ACCase mutein encoded In amino acid sequence by replacing and/or one or more amino acid being deleted and/or added, and the mutein has Activity of acetyl coenzyme A carboxylase.

4. a kind of ACCase mutated genes, it is characterised in that:

(i) it encodes described in any item albumen according to claim 1 ~ 2；Or

(ii) there is activity of acetyl coenzyme A carboxylase with the nucleotide sequence hybridization and coding (i) limited under strict conditions The nucleotide sequence of protein；Or

Its nucleotide sequence be selected from SEQ ID NO:1 or SEQ ID NO:3 or SEQ ID NO:5 or SEQ ID NO:7 or In SEQ ID NO:9 or SEQ ID NO:11 or SEQ ID NO:13 or SEQ ID NO:15.

5. ACCase mutated genes according to claim 4, which is characterized in that lead the ACCase mutated genes Enter plant, can make recipient plant that there is the resistance to herbicide.

6. ACCase mutated genes according to claim 5, which is characterized in that the herbicide is diclofop-methyl and lid grass One of energy is a variety of.

7. ACCase mutated genes according to claim 5, which is characterized in that lead the ACCase mutated genes The method for entering recipient plant is transgenosis, hybridization or backcrossing.

8. ACCase mutated genes according to claim 7, which is characterized in that the transgenic method, including it is as follows Step:

1) expression vector and expression cassette containing ACCase mutated genes are constructed；

2) it is mutated plant comprising ACCase according to claim 4 by genetic transformation wheat above-mentioned expression vector Type gene；

3) plant is made to express wheat ACCase mutein according to claim 1 or 2.

9. a kind of recombinant vector and expression cassette containing ACCase mutated genes according to claim 4.

10. a kind of host cell of the expression vector containing ACCase mutated genes according to claim 4.