CN109355253A - Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge - Google Patents
Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge Download PDFInfo
- Publication number
- CN109355253A CN109355253A CN201811410285.0A CN201811410285A CN109355253A CN 109355253 A CN109355253 A CN 109355253A CN 201811410285 A CN201811410285 A CN 201811410285A CN 109355253 A CN109355253 A CN 109355253A
- Authority
- CN
- China
- Prior art keywords
- cell
- culture medium
- culture
- umbilical cord
- mesenchymal stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1369—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
Abstract
The present invention relates to cell Fiber differentiation technical field, culture medium used in the cultural method and this method that break up in particular to a kind of inducing umbilical cord mesenchymal stem at rouge.The culture medium is obtained by least additionally adding following component in basal medium: 0.7~1.3 μm of ol/L of dexamethasone, 40~60 μ g/mL of 400~600 μm of ol/L, IBMX0.3~0.7mmol/L of Indomethacin, 7~13 μm of ol/L of insulin and acid ascorbyl ester.The present invention cooperates specific cultural method by optimization inducing component, improves induced velocity and efficiency, induced efficiency is high i.e. it can be seen that a large amount of intracellular fat drips are formed within 5~7 days.With induction time, fat drips are become larger, and illustrate that lipoblast function is good.It dyes within 12~14 days, it can be seen that very classical red oil droplet.
Description
Technical field
The present invention relates to cell Fiber differentiation technical fields, in particular to a kind of inducing umbilical cord mesenchymal stem
Culture medium used in cultural method and this method at rouge differentiation.
Background technique
Mescenchymal stem cell (mesenchymal stem cells, MSC) be mesoderma origin, have height self more
The multipotential stem cell of new ability and multi-lineage potential.In fact, MSC is widely present in whole body Various Tissues, almost source
In all histoorgans of body, such as marrow, periosteum, adipose tissue, dental pulp, synovial membrane, umbilical cord, placenta, amniotic fluid and fetal tissue
Deng.The mescenchymal stem cell of separate sources has similar form, expresses identical surface markers, has similar biological characteristics
Property aspect, can such as cultivate amplification in vitro, and can be divided under given conditions thin including nerve cell, osteoblast, cartilage
The cell of more organization systems including born of the same parents, muscle cell, fat cell.MSC in addition to multidirectional histocyte differentiation capability it
Outside, important regulative also is played in hematopoiesis, immunization inflammatory reaction, angiogenesis et al. body critical function.
Umbilical cord mesenchymal stem cells, which refer to, is present in one of neonatal umbilical cord tissue versatile stem cell.It is filled between umbilical cord
Matter is a kind of low immunogenicity cell, and has very strong immunoloregulation function.Umbilical cord mesenchymal stem cells possess the whole of MSC
Characteristic, and rich content are easily isolated culture.
Compared with the MSC in other sources, UC-MSC is more original, is dry between embryonic stem cell and adult stem cell
Cell, proliferation is lower than embryonic stem cell with differentiation capability, but is apparently higher than adult stem cell.
Umbilical cord mesenchymal stem cells have more differentiation potentials, and under specific inductive condition, UC-MSC can not only be divided into
The mesoblastemas such as bone, cartilage, fat, tendon, and can inwardly embryonic tissue cell (such as cardiac muscle cell, liver cell) and outside
Embryonic tissue cell (such as nerve cell) differentiation.UC-MSC does not have oncogenicity, can different inductive condition and it is suitable in vivo
It grows in microenvironment, safely directed differentiation is different tissue lines, has the ability for repairing various tissues and organ.
It is the complex process controlled by many factors that umbilical cord mesenchymal stem cells, which are induced differentiation into fat cell, is had
Multiple genes participate in regulation, and the differentiation at rouge direction is and the result that is activated at a series of relevant transcription factors of rouge.The factor
Mainly have: Rev-erb-a, NOTCH, Kruppe1 like factor -15 (KLF150, liver X receptor (LXRs0,3- Hydroxymethylglutaryl list
Acyl CoA synthase (HMCS), cAMP-responsive element binding protein/antibody (CREB), STAT etc..
At fatty stimulant also there are many kinds of, in the medium add dexamethasone, the urgent xanthine of 3- isobutyl group -1-
(IBMX), insulin and indocin etc. can induce umbilical cord mesenchymal stem cells to Adipose Differentiation.
Umbilical cord mesenchymal stem cells are that mescenchymal stem cell one differentiation relatively difficult to achieve is tested at rouge differentiation, induction group
Divide and the formation of fat cell is had significant effect.Current inductive technology, it is extremely low at rouge differentiation rate, only filled between only a few
Matter stem cell can break up lipoblast, form fat drips, remaining most cells can not form fat drips always.
The size of fat drips is also to judge into the evaluation index of rouge differentiation superiority and inferiority, and most of method of inducing differentiation can be only formed pole
Small distributed fat drips illustrate that culture scheme can not be that mescenchymal stem cell sufficiently breaks up lipoblast.
In view of this, the present invention is specifically proposed.
Summary of the invention
It is a primary object of the present invention to be induced to differentiate into fat induction in the process by optimizing umbilical cord mesenchymal stem cells
Break up component, improve speed and ratio that umbilical cord mesenchymal stem cells are divided into fat cell, improves fat cell after inducing
Quality.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
The present invention relates to the culture medium that a kind of inducing umbilical cord mesenchymal stem breaks up at rouge, by basal medium extremely
Following component is additionally added less to obtain:
0.7~1.3 μm of ol/L of dexamethasone, Indomethacin 400~600 μm of ol/L, IBMX0.3~0.7mmol/L, pancreas islet
40~60 μ g/mL of element 7~13 μm of ol/L and acid ascorbyl ester.
According to another aspect of the present invention, the invention further relates to a kind of inducing umbilical cord mesenchymal stems into rouge differentiation side
Method, comprising:
Use culture medium culture umbilical cord mesenchymal stem cells as described above.
Compared with prior art, the invention has the benefit that
The present invention cooperates specific cultural method, improves induced velocity and efficiency by optimization inducing component, and 5~7 days
I.e. it can be seen that a large amount of intracellular fat drips are formed, induced efficiency is high.With induction time, fat drips are become larger, and it is thin to be illustrated as rouge
Born of the same parents' function is good.It dyes within 12~14 days, it can be seen that very classical red oil droplet.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the Oil red O stained photographs of fat cell cultivated in one embodiment of the invention, × 10；
Fig. 2 is the Oil red O stained photographs of fat cell cultivated in one embodiment of the invention, × 20；
Fig. 3 is the Oil red O stained photographs of fat cell cultivated in one embodiment of the invention, × 40.
Specific embodiment
The present invention relates to the culture medium that a kind of inducing umbilical cord mesenchymal stem breaks up at rouge, by basal medium extremely
Following component is additionally added less to obtain:
0.7~1.3 μm of ol/L of dexamethasone, Indomethacin 400~600 μm of ol/L, IBMX0.3~0.7mmol/L, pancreas islet
40~60 μ g/mL of element 7~13 μm of ol/L and acid ascorbyl ester.
In some embodiments, it is obtained by least additionally adding following component in basal medium:
0.8~1.2 μm of ol/L of dexamethasone, Indomethacin 450~550 μm of ol/L, IBMX0.4~0.6mmol/L, pancreas islet
45~55 μ g/mL of element 8~12 μm of ol/L and acid ascorbyl ester.
In some embodiments, it is obtained by least additionally adding following component in basal medium:
1 μm of ol/L of dexamethasone, 500 μm of ol/L, IBMX0.5mmol/L of Indomethacin, 10 μm of ol/L of insulin and anti-
Bad 50 μ g/mL of hematic acid ester.
In some embodiments, the basal medium is DMEM low sugar culture medium；
In some embodiments, in the DMEM low sugar culture medium containing 8%~12%FBS and 0.08%~
0.12% glutamine.
DMEM low sugar culture medium may be selected to be 86%~92% low sugar DMEM, and it is dry that low sugar culture medium is suitable for umbilical cord mesenchyma
Differentiation of the cell to fat cell.
According to another aspect of the present invention, the invention further relates to a kind of inducing umbilical cord mesenchymal stems into rouge differentiation side
Method, comprising:
Use culture medium culture umbilical cord mesenchymal stem cells as described above.
It in some embodiments, is P3~P5 generation for the umbilical cord mesenchymal stem cells of inoculation, cell confluency degree is
70%~80% and cell in good condition collect and obtain after digesting.
The umbilical cord mesenchymal stem cells growth of this cultivation stage rapidly, is conducive to differentiation culture.
In some embodiments, pancreas enzyme concentration used by the digestion is 0.20%~0.30%；It is also an option that
0.25%.
In some embodiments, it is completed in digestion, after cleaning cell, cell is moved into ventilative sharp floor cells culture tube
In, supernatant liquid is removed after centrifugation.
In some embodiments, in culture, the inoculum concentration of cell is every 2mL culture medium inoculated (1~5) × 104It is a
Cell.
In some embodiments, the inoculum concentration of cell is every 2mL culture medium inoculated (2~4) × 104A cell；Or 3 ×
104A cell.
In some embodiments, the time of the culture is 12~14 days, it is also an option that 13 days；
In some embodiments, it changes the liquid once within every 2~4 days.
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
It can be with conventional products that are commercially available.
Embodiment 1
1. inducing component
2. the preparation of induced medium
1) basal medium of induction differentiation is 89% low sugar DMEM, 10%FBS fetal calf serum and 1% glutamine.Its
Glutamine faces used time addition.
2) with prepare the complete induced medium of 100mL calculate inducing component dexamethasone, Indomethacin, IBMX, insulin,
The dosage of acid ascorbyl ester is dispensed with sterile 0.5mLEP pipe, -20 DEG C of preservations.All components are kept in dark place.
3) by the basal medium of required volume, dexamethasone, Indomethacin, IBMX, insulin, ascorbic acid when preparing
Ester takes out, and mixes after dissolving, is made into complete medium, can 4 DEG C of preservation 5-7d.
3. inducing differentiation step
1) T75 bottles of inoculations are taken, in P3-P5 generation, covers with to 70-80% and in good condition one bottle of umbilical cord mesenchymal stem cells,
Pipette sucks culture medium, and 2mLPBS is added, and gently spin rinse falls remaining culture medium.
2) 0.25% pancreatin that 1mL shifts to an earlier date equilibrium at room temperature is added, gently rotation is uniformly distributed pancreatin.Lay flat static 1-
2min or so lifts culture bottle and visually observes whether adherent cell is mottled to fall off under lamp.It, can be gently if digestion process is slower
Culture bottle side wall is patted, cell detachment is accelerated by vibration.
3) cell is substantially all fall off after, be added 1mL serum, rotation mixing terminate pancreatin digestion.It is added
10mLPBS collects cell, and the cell of collection is transferred in 50mL centrifuge tube.10mLPBS is added again, rotating and culturing bottle is received
Collect remaining cell.
4) it is placed in a centrifuge, trim, 1000rpm is centrifuged 5min, gently sucks supernatant.20mLPBS is added again
1000rpm is centrifuged 5min, gently sucks supernatant.
5) cell is resuspended in 10mL basal medium, is counted with cell counting board, calculates cell density.
6) 3 × 10 are taken4Umbilical cord mesenchymal stem cells in diameter 9cm Tissue Culture Dish, basal medium is added to end
Volume 2ml, shaking gently culture dish is uniformly distributed cell in entire culture dish.
7) Tissue Culture Dish is gently placed in CO2In incubator, culture overnight keeps cell adherent.
8) it siphons away basal medium within second day, 2mL induced medium is gently added, to start to induce D0.
9) it changes the liquid once within every 3 days, while observing cell state.
5-7 days i.e. it can be seen that fat drips are formed.
12-14 days part fat drips are very big, should be dyed at this time, if delay fat drips may rupture.
Embodiment 2
1. inducing component
2. the preparation of induced medium
With embodiment 1.
3. inducing differentiation step
With embodiment 1.
Embodiment 3
1. inducing component
| Title | Content |
| Dexamethasone | 0.9μmol/L |
| Indomethacin | 550μmol/L |
| IBMX | 0.6mmol/L |
| Insulin | 11μmol/L |
| Acid ascorbyl ester | 45μg/mL |
2. the preparation of induced medium
With embodiment 1.
3. inducing differentiation step
With embodiment 1.
Embodiment 4
1. inducing component
| Title | Content |
| Dexamethasone | 1μmol/L |
| Indomethacin | 500μmol/L |
| IBMX | 0.5mmol/L |
| Insulin | 10μmol/L |
| Acid ascorbyl ester | 50μg/mL |
2. the preparation of induced medium
With embodiment 1.
3. inducing differentiation step
With embodiment 1.
Experimental example
Resulting fat cell is broken up to the culture of embodiment 4 and carries out oil red O stain identification.
1) by the neutral filter paper filtering of oil red O working solution, remove impurity therein.
2) induced medium in culture dish is siphoned away, 2mLPBS is gently added and rinses 2 times.
3) the fixed 30min of the center 2mL4% formaldehyde is added, sucks formaldehyde, 2mLPBS is gently added and rinses 2 times.
4) 1mL oil red O working solution is added, dyes 30min.
5) oil red O dye liquor is siphoned away, is cleaned 2 times with 1mLPBS.
6) microscopically observation is taken pictures.
Result of taking pictures is as shown in Figures 1 to 3.It can see by microscope macrograph, umbilical cord mesenchymal stem cells are in large quantities
It is divided into lipoblast, differentiation ratio is very high.High power lens photo can see with the presence of a large amount of big fat drips, be illustrated as rouge point
Change state is very good.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, but those skilled in the art should understand that: its
It is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features
It is equivalently replaced；And these are modified or replaceed, various embodiments of the present invention skill that it does not separate the essence of the corresponding technical solution
The range of art scheme.
Claims (10)
1. the culture medium that a kind of inducing umbilical cord mesenchymal stem breaks up at rouge, which is characterized in that its by basal medium extremely
Following component is additionally added less to obtain:
0.7~1.3 μm of ol/L of dexamethasone, 400~600 μm of ol/L, IBMX0.3~0.7mmol/L of Indomethacin, insulin 7
40~60 μ g/mL of~13 μm of ol/L and acid ascorbyl ester.
2. culture medium according to claim 1, which is characterized in that it is by addition such as the following group at least additional in basal medium
Get:
0.8~1.2 μm of ol/L of dexamethasone, 450~550 μm of ol/L, IBMX0.4~0.6mmol/L of Indomethacin, insulin 8
45~55 μ g/mL of~12 μm of ol/L and acid ascorbyl ester.
3. culture medium according to claim 1, which is characterized in that it is by addition such as the following group at least additional in basal medium
Get:
1 μm of ol/L of dexamethasone, 500 μm of ol/L, IBMX0.5mmol/L of Indomethacin, 10 μm of ol/L of insulin and Vitamin C
50 μ g/mL of acid esters.
4. described in any item culture mediums according to claim 1~3, which is characterized in that the basal medium is DMEM low sugar
Culture medium；
Preferably, the glutamine containing 8%~12%FBS and 0.08%~0.12% in the DMEM low sugar culture medium.
5. a kind of inducing umbilical cord mesenchymal stem is at rouge differentiation method characterized by comprising
Use the described in any item culture medium culture umbilical cord mesenchymal stem cells of Claims 1 to 44.
6. according to the method described in claim 5, it is characterized in that, the umbilical cord mesenchymal stem cells for inoculation are P3~P5
In generation, cell confluency degree is 70%~80% and cell in good condition is collected after digesting and obtained.
7. according to the method described in claim 6, it is characterized in that, pancreas enzyme concentration used by the digestion be 0.20%~
0.30%.
8. after cleaning cell, cell is moved to ventilative the method according to the description of claim 7 is characterized in that being completed in digestion
In sharp floor cells culture tube, supernatant liquid is removed after centrifugation.
9. according to the method described in claim 5, it is characterized in that, the inoculum concentration of cell is that every 2mL culture medium connects in culture
Kind (1~5) × 104A cell.
10. according to the method described in claim 5, it is characterized in that, the time of the culture is 12~14 days；Preferably, every 2
It changes the liquid once within~4 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811410285.0A CN109355253A (en) | 2018-11-23 | 2018-11-23 | Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811410285.0A CN109355253A (en) | 2018-11-23 | 2018-11-23 | Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109355253A true CN109355253A (en) | 2019-02-19 |
Family
ID=65338609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811410285.0A Pending CN109355253A (en) | 2018-11-23 | 2018-11-23 | Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109355253A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172101A (en) * | 2019-12-31 | 2020-05-19 | 上海林望生物科技有限公司 | Method for inducing and differentiating human pluripotent stem cells into adipocytes |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110104133A1 (en) * | 2008-05-06 | 2011-05-05 | Joslin Diabetes Center, Inc. | Methods and compositions for inducing brown adipogenesis |
| CN104830757A (en) * | 2015-04-15 | 2015-08-12 | 广州赛莱拉干细胞科技股份有限公司 | Mesenchymal stem cell adipogenic induced differentiation culture medium and preparation method thereof |
| WO2017009263A1 (en) * | 2015-07-10 | 2017-01-19 | Etablissement Francais Du Sang | Method for obtaining human brown/beige adipocytes |
| CN107267450A (en) * | 2017-08-08 | 2017-10-20 | 成都源泉生物科技有限公司 | A kind of adult stem cell adipogenic induction culture medium |
| CN108588015A (en) * | 2018-03-06 | 2018-09-28 | 安徽瑞杰赛尔生物科技有限公司 | A kind of human mesenchymal stem cell adipogenic induction differential medium and preparation method |
-
2018
- 2018-11-23 CN CN201811410285.0A patent/CN109355253A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110104133A1 (en) * | 2008-05-06 | 2011-05-05 | Joslin Diabetes Center, Inc. | Methods and compositions for inducing brown adipogenesis |
| CN104830757A (en) * | 2015-04-15 | 2015-08-12 | 广州赛莱拉干细胞科技股份有限公司 | Mesenchymal stem cell adipogenic induced differentiation culture medium and preparation method thereof |
| WO2017009263A1 (en) * | 2015-07-10 | 2017-01-19 | Etablissement Francais Du Sang | Method for obtaining human brown/beige adipocytes |
| CN107267450A (en) * | 2017-08-08 | 2017-10-20 | 成都源泉生物科技有限公司 | A kind of adult stem cell adipogenic induction culture medium |
| CN108588015A (en) * | 2018-03-06 | 2018-09-28 | 安徽瑞杰赛尔生物科技有限公司 | A kind of human mesenchymal stem cell adipogenic induction differential medium and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 唐军 等: "低氧对人脐带间充质干细胞成脂肪分化的影响", 《中国美容医学》 * |
| 汪兆艳 等: "人脐带源间充质干细胞体外成脂、成骨、成软骨诱导分化", 《转化医学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111172101A (en) * | 2019-12-31 | 2020-05-19 | 上海林望生物科技有限公司 | Method for inducing and differentiating human pluripotent stem cells into adipocytes |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101748096B (en) | Sub totipotential stem cell and preparation method and application thereof | |
| CN104450611B (en) | A kind of primary isolation and culture method of human amnion mesenchymal stem cell | |
| CN102367435B (en) | Preparation of human platelet-rich plasma and application of same in isolation and culture of human mesenchymal stem cells | |
| CN102978156A (en) | Expansion in vitro purification culture method of mesenchymal stem cells and culture medium | |
| CN109456939A (en) | Culture medium used in cultural method and this method of the inducing umbilical cord mesenchymal stem at cartilage differentiation | |
| CN104762257B (en) | A kind of method preparing mescenchymal stem cell from umbilical cord | |
| CN109234229A (en) | Method and digestive enzyme compositions used from placenta blood vessel separating mesenchymal stem cell | |
| CN105039248B (en) | Tree shrew mesenchymal stem cell culture systems | |
| CN109988746A (en) | A kind of mescenchymal stem cell adipogenic induction differentiation method | |
| CN105087474A (en) | Culture method of deciduous tooth pulp stem cells | |
| CN105850979A (en) | Cryoprotective solution and cryopreservation method for bone mesenchymal stem cells | |
| CN105062970B (en) | A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast | |
| CN106754685A (en) | A kind of construction method of Human fat mesenchymal stem cell bank | |
| CN106119191A (en) | A kind of placental villi plate mescenchymal stem cell and clinicization preparation method | |
| CN107287156A (en) | A kind of isolated culture method of fat mesenchymal stem cell and its application | |
| CN109628388B (en) | Isolation of mesenchymal stem cells from placental blood vessels with digestive enzyme composition | |
| CN104450610B (en) | Subculture method of human amniotic mesenchymal stem cells | |
| CN107236705B (en) | Human placenta chorion mesenchymal stem cell culture system | |
| CN109355253A (en) | Culture medium used in the cultural method and this method that inducing umbilical cord mesenchymal stem breaks up at rouge | |
| CN104031881B (en) | A kind of isolated culture method of mankind's olfactory mucosa mescenchymal stem cell | |
| CN107287158A (en) | The method of effective acquisition mescenchymal stem cell from mouse dense bone | |
| CN102703380B (en) | Sub-totipotent stem cell, preparation method and application thereof | |
| CN106032529A (en) | Method for in-vitro separation, amplification and induced differentiation of placenta-derived mesenchymal stem cells | |
| CN108495924A (en) | Method for generating mescenchymal stem cell | |
| CN108774630A (en) | A kind of primary culture method of Microhyla ornata Skeletal Muscle Cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: Room D409, floor 4, building 1, No. 12, Shangdi Information Road, Haidian District, Beijing 100000 Applicant after: Beijing Taidong Biotechnology Co., Ltd. Address before: 100000 Floor C54, No. 19, Yufangyuan District, Fengtai District, Beijing Applicant before: Beijing Taidong Biotechnology Co., Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190219 |