CN105062970B - A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast - Google Patents
A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast Download PDFInfo
- Publication number
- CN105062970B CN105062970B CN201510525294.4A CN201510525294A CN105062970B CN 105062970 B CN105062970 B CN 105062970B CN 201510525294 A CN201510525294 A CN 201510525294A CN 105062970 B CN105062970 B CN 105062970B
- Authority
- CN
- China
- Prior art keywords
- cell
- induction
- stem cell
- induced
- neuroblast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000006698 induction Effects 0.000 title claims abstract description 71
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 35
- 230000004069 differentiation Effects 0.000 title claims abstract description 20
- 210000003757 neuroblast Anatomy 0.000 title claims abstract description 14
- 241001597008 Nomeidae Species 0.000 title abstract description 13
- 230000001939 inductive effect Effects 0.000 claims abstract description 27
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims abstract description 19
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims abstract description 18
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims abstract description 13
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims abstract description 13
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims abstract description 12
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims abstract description 12
- 235000021283 resveratrol Nutrition 0.000 claims abstract description 12
- 229940016667 resveratrol Drugs 0.000 claims abstract description 12
- TZJALUIVHRYQQB-XFDQAQKOSA-N Icariin Natural products O(C)c1ccc(C2=C(O[C@H]3[C@@H](O)[C@H](O)[C@@H](O)[C@H](C)O3)C(=O)c3c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O4)c(C/C=C(\C)/C)c3O2)cc1 TZJALUIVHRYQQB-XFDQAQKOSA-N 0.000 claims abstract description 9
- 229960000890 hydrocortisone Drugs 0.000 claims abstract description 9
- TZJALUIVHRYQQB-XLRXWWTNSA-N icariin Chemical compound C1=CC(OC)=CC=C1C1=C(O[C@H]2[C@@H]([C@H](O)[C@@H](O)[C@H](C)O2)O)C(=O)C2=C(O)C=C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)C(CC=C(C)C)=C2O1 TZJALUIVHRYQQB-XLRXWWTNSA-N 0.000 claims abstract description 9
- TZJALUIVHRYQQB-UHFFFAOYSA-N icariine Natural products C1=CC(OC)=CC=C1C1=C(OC2C(C(O)C(O)C(C)O2)O)C(=O)C2=C(O)C=C(OC3C(C(O)C(O)C(CO)O3)O)C(CC=C(C)C)=C2O1 TZJALUIVHRYQQB-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000470 constituent Substances 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 13
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 9
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 claims description 9
- 239000004017 serum-free culture medium Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 106
- 239000003102 growth factor Substances 0.000 abstract description 22
- 210000002569 neuron Anatomy 0.000 abstract description 22
- 238000000034 method Methods 0.000 abstract description 15
- 239000003814 drug Substances 0.000 abstract description 11
- 238000002054 transplantation Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 2
- 210000003061 neural cell Anatomy 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 21
- 102000004243 Tubulin Human genes 0.000 description 20
- 108090000704 Tubulin Proteins 0.000 description 20
- 208000036815 beta tubulin Diseases 0.000 description 20
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 16
- 210000003954 umbilical cord Anatomy 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 11
- 239000000411 inducer Substances 0.000 description 11
- 210000001519 tissue Anatomy 0.000 description 10
- 102100039289 Glial fibrillary acidic protein Human genes 0.000 description 9
- 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 9
- 101001092197 Homo sapiens RNA binding protein fox-1 homolog 3 Proteins 0.000 description 9
- 102100035530 RNA binding protein fox-1 homolog 3 Human genes 0.000 description 9
- 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 9
- 229910002092 carbon dioxide Inorganic materials 0.000 description 8
- 239000001569 carbon dioxide Substances 0.000 description 8
- 102100028471 Eosinophil peroxidase Human genes 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 210000000577 adipose tissue Anatomy 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000001413 cellular effect Effects 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 210000005056 cell body Anatomy 0.000 description 5
- 208000024908 graft versus host disease Diseases 0.000 description 5
- 238000010166 immunofluorescence Methods 0.000 description 5
- 210000005036 nerve Anatomy 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000004040 coloring Methods 0.000 description 4
- 230000001079 digestive effect Effects 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 229930182470 glycoside Natural products 0.000 description 4
- 150000002338 glycosides Chemical class 0.000 description 4
- 210000003716 mesoderm Anatomy 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 230000000007 visual effect Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000004255 Butylated hydroxyanisole Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241001494479 Pecora Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 210000004204 blood vessel Anatomy 0.000 description 3
- 235000019282 butylated hydroxyanisole Nutrition 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 230000007969 cellular immunity Effects 0.000 description 3
- 230000009194 climbing Effects 0.000 description 3
- 150000001886 cortisols Chemical class 0.000 description 3
- 230000000093 cytochemical effect Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000003125 immunofluorescent labeling Methods 0.000 description 3
- 230000004130 lipolysis Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000003182 parenteral nutrition solution Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 102100022464 5'-nucleotidase Human genes 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108010092408 Eosinophil Peroxidase Proteins 0.000 description 2
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 2
- 208000009329 Graft vs Host Disease Diseases 0.000 description 2
- 102000006354 HLA-DR Antigens Human genes 0.000 description 2
- 108010058597 HLA-DR Antigens Proteins 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101000935043 Homo sapiens Integrin beta-1 Proteins 0.000 description 2
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 description 2
- 102100025304 Integrin beta-1 Human genes 0.000 description 2
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 2
- 208000001738 Nervous System Trauma Diseases 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000001994 activation Methods 0.000 description 2
- 206010000891 acute myocardial infarction Diseases 0.000 description 2
- 210000000845 cartilage Anatomy 0.000 description 2
- 239000002458 cell surface marker Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 239000006059 cover glass Substances 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000007443 liposuction Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 208000028412 nervous system injury Diseases 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical compound C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 206010007559 Cardiac failure congestive Diseases 0.000 description 1
- 206010007710 Cartilage injury Diseases 0.000 description 1
- 206010061762 Chondropathy Diseases 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 208000011231 Crohn disease Diseases 0.000 description 1
- 102100037241 Endoglin Human genes 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 241001016310 Epimedium grandiflorum Species 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 1
- 230000010558 Gene Alterations Effects 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010018473 Glycosuria Diseases 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 101000881679 Homo sapiens Endoglin Proteins 0.000 description 1
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 1
- 101000994375 Homo sapiens Integrin alpha-4 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 208000023105 Huntington disease Diseases 0.000 description 1
- 206010061216 Infarction Diseases 0.000 description 1
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 1
- 102100032818 Integrin alpha-4 Human genes 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 102000015336 Nerve Growth Factor Human genes 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 208000004680 Rectal Fistula Diseases 0.000 description 1
- 206010061363 Skeletal injury Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 206010002156 anal fistula Diseases 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 210000001130 astrocyte Anatomy 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- CZBZUDVBLSSABA-UHFFFAOYSA-N butylated hydroxyanisole Chemical compound COC1=CC=C(O)C(C(C)(C)C)=C1.COC1=CC=C(O)C=C1C(C)(C)C CZBZUDVBLSSABA-UHFFFAOYSA-N 0.000 description 1
- 229940043253 butylated hydroxyanisole Drugs 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 210000000080 chela (arthropods) Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 235000018905 epimedium Nutrition 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000012637 gene transfection Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000007574 infarction Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 230000005405 multipole Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- 229940053128 nerve growth factor Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000008354 sodium chloride injection Substances 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 208000020431 spinal cord injury Diseases 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 210000001113 umbilicus Anatomy 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510525294.4A CN105062970B (en) | 2015-08-25 | 2015-08-25 | A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510525294.4A CN105062970B (en) | 2015-08-25 | 2015-08-25 | A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105062970A CN105062970A (en) | 2015-11-18 |
CN105062970B true CN105062970B (en) | 2017-12-29 |
Family
ID=54492489
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510525294.4A Active CN105062970B (en) | 2015-08-25 | 2015-08-25 | A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105062970B (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107058225B (en) * | 2017-02-10 | 2020-06-23 | 郑州大学 | Compound induction culture medium and method for inducing umbilical cord mesenchymal stem cells into neuron-like cells by adopting culture medium |
CN109852654B (en) * | 2018-12-28 | 2021-02-26 | 广州润虹医药科技股份有限公司 | Composition capable of inducing stem cells to secrete cytokines and application thereof |
CN109694851B (en) * | 2019-01-16 | 2021-03-16 | 吉林省拓华生物科技有限公司 | Inducing composition and inducing differentiation culture solution of human mesenchymal stem cells, and in-vitro inducing method and application thereof |
CN113106054B (en) * | 2020-01-13 | 2022-10-14 | 青岛瑞思德生物科技有限公司 | Inducer for inducing and differentiating mesenchymal stem cells into islet cells |
CN111394311B (en) | 2020-04-20 | 2022-07-01 | 青岛瑞思德生物科技有限公司 | Serum-free complete culture medium for inducing mesenchymal stem cells to differentiate into corneal epithelial cells |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103087983A (en) * | 2011-10-27 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and application |
CN103215222A (en) * | 2013-04-19 | 2013-07-24 | 陈云燕 | Induction medium for inducing human adipose tissue-derived stromal cells as nerve cells and method |
CN104419661A (en) * | 2013-08-28 | 2015-03-18 | 中国医学科学院基础医学研究所 | Preparation method of neural stem cell |
CN104726406A (en) * | 2015-02-13 | 2015-06-24 | 中国医科大学 | Method for inducing dental pulp mesenchymal stem cells to be differentiated into nerve cells |
CN104789531A (en) * | 2015-03-27 | 2015-07-22 | 安沂华 | Method for inducing umbilical cord mesenchymal stem cells to be differentiated into dopaminergic neurons |
-
2015
- 2015-08-25 CN CN201510525294.4A patent/CN105062970B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103087983A (en) * | 2011-10-27 | 2013-05-08 | 北京清美联创干细胞科技有限公司 | Method for inducing proliferation and differentiation of muscle mesenchymal stem cells by medicines, and application |
CN103215222A (en) * | 2013-04-19 | 2013-07-24 | 陈云燕 | Induction medium for inducing human adipose tissue-derived stromal cells as nerve cells and method |
CN104419661A (en) * | 2013-08-28 | 2015-03-18 | 中国医学科学院基础医学研究所 | Preparation method of neural stem cell |
CN104726406A (en) * | 2015-02-13 | 2015-06-24 | 中国医科大学 | Method for inducing dental pulp mesenchymal stem cells to be differentiated into nerve cells |
CN104789531A (en) * | 2015-03-27 | 2015-07-22 | 安沂华 | Method for inducing umbilical cord mesenchymal stem cells to be differentiated into dopaminergic neurons |
Non-Patent Citations (6)
Title |
---|
Differentiation into neurons of rat bone marrow-derived mesenchymal stem cells;Ming Guan 等;《Eur.Cytokine Netw.》;20141231;2793-2796 * |
Directional differentiation of bone marrow mesenchymal stem cells into oligodendrocytes induced by the combination of various neurotrophic factors;Xu Yong-tao 等;《Journal of Clinical Rehabilitative Tissue Engineering Research》;20090402;58-63 * |
不同方法诱导大鼠骨髓间充质干细胞向神经样细胞的分化;徐路尧 等;《苏州大学学报(医学版)》;20091231;197-201 * |
促红细胞生成素基因修饰脐带间充质干细胞移植颅脑损伤大鼠脑细胞形态及神经功能的恢复;钟志坚 等;《中国组织工程研究》;20120205;1036-1040 * |
无血清培养条件诱导人脐血间充质干细胞向神经细胞的分化;涂怀军 等;《中国组织工程研究与临床康复》;20090205;1035-1038 * |
淫羊藿苷介导MAPK信号通路促进间充质干细胞株C3H10T1/2成骨分化的体外研究;毛项颖 等;《中西医结合学报》;20121130;1272-1278 * |
Also Published As
Publication number | Publication date |
---|---|
CN105062970A (en) | 2015-11-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105062970B (en) | A kind of derivant and induction differentiation complete medium that mescenchymal stem cell is induced to neuroblast | |
CN102127522B (en) | Human umbilical mesenchymal stem cell and preparation method thereof | |
CN101748096B (en) | Sub totipotential stem cell and preparation method and application thereof | |
CN105132369B (en) | A kind of derivant and inducing culture that mescenchymal stem cell is converted into testosterone secretion cell | |
CN104630144B (en) | A kind of separation of umbilical cord blood mesenchymal stem cellses and cultural method | |
CN106062179A (en) | Serum-free medium | |
CN104762257B (en) | A kind of method preparing mescenchymal stem cell from umbilical cord | |
CN107921182A (en) | Mechanical device and method for isolation medium vasculature part | |
CN108300690A (en) | A kind of isolated culture method and serum free medium of fat mesenchymal stem cell | |
CN103301154B (en) | Application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus | |
CZ301148B6 (en) | Method of culturing human mezenchymal stem cells, particularly for facilitating fracture healing process, and bioreactor for carrying out the method | |
RU2433172C2 (en) | Method of obtaining homogenous population of stem cells and its application | |
CN108192862A (en) | A kind of preparation method of pilose antler stem cell, pilose antler stem cell and its application | |
CN104651305A (en) | Method for acquiring bioactive proteins by utilizing umbilical cord mesenchymal stem cells | |
CN107267453A (en) | A kind of culture medium and its application for being used to cultivate fat mesenchymal stem cell | |
CN109402175A (en) | The fat stem cell and preparation method of expression chemokine receptors CCR2B and application | |
CN103215222A (en) | Induction medium for inducing human adipose tissue-derived stromal cells as nerve cells and method | |
CN107287156A (en) | A kind of isolated culture method of fat mesenchymal stem cell and its application | |
CN105112367B (en) | A kind of mescenchymal stem cell epidermal differentiation derivant and its application process | |
CN104031881B (en) | A kind of isolated culture method of mankind's olfactory mucosa mescenchymal stem cell | |
CN108865985A (en) | A kind of method of the pre- epithelial-mesenchymal conversion of stem cell source excretion soma | |
CN102703380B (en) | Sub-totipotent stem cell, preparation method and application thereof | |
CN106978395A (en) | A kind of method for efficiently separating culture umbilical cord mesenchymal stem cells | |
CN106032529A (en) | Method for in-vitro separation, amplification and induced differentiation of placenta-derived mesenchymal stem cells | |
CN115820540A (en) | Endothelial differentiation inducer for mesenchymal stem cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160824 Address after: 266109, No. 368, Hedong Road, hi tech Zone, Shandong, Qingdao, Qingdao 101, room 7, blue bio Pharmaceutical Industrial Park Applicant after: QINGDAO RUISIKE BIOTECHNOLOGY CO.,LTD. Address before: 101200 Pinggu City, North Street, Beijing District, building No. 13, room No. 802 Applicant before: BEIJING RE-STORE BIOMEDICINE RESEARCH INSTITUTE |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181119 Address after: 266000 1st and 2nd floors, 7th Building, Incubation Center of Qingdao Blue Biomedical Industrial Park, 368 Hedong Road, Qingdao Hi-tech Zone, Qingdao City, Shandong Province Patentee after: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Address before: 266109 Qingdao No. 368 Hedong Road, Qingdao high tech Zone, Shandong, 101, room 7, blue biomedical industry park. Patentee before: QINGDAO RUISIKE BIOTECHNOLOGY CO.,LTD. |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to an inducer for inducing mesenchymal stem cells into nerve cells and a complete culture medium for inducing differentiation Effective date of registration: 20220325 Granted publication date: 20171229 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010040 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230328 Granted publication date: 20171229 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010040 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An inducer for inducing mesenchymal stem cells into neural cells and inducing complete differentiation medium Effective date of registration: 20230329 Granted publication date: 20171229 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023370010033 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20171229 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023370010033 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An inducer and fully differentiated culture medium for inducing mesenchymal stem cells into neural cells Granted publication date: 20171229 Pledgee: Qingdao Huitong Chinese financing Company limited by guarantee Pledgor: QINGDAO RESTORE BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024370010043 |