CN109349628A - A kind of sophora flower taste soda water and preparation method thereof - Google Patents
A kind of sophora flower taste soda water and preparation method thereof Download PDFInfo
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- CN109349628A CN109349628A CN201811282267.9A CN201811282267A CN109349628A CN 109349628 A CN109349628 A CN 109349628A CN 201811282267 A CN201811282267 A CN 201811282267A CN 109349628 A CN109349628 A CN 109349628A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/40—Effervescence-generating compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/56—Flavouring or bittering agents
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Abstract
The invention belongs to food processing technology field, a kind of sophora flower taste soda water and preparation method thereof is disclosed, is made of according to mass percent sodium bicarbonate 3.0%, sophora flower essence 0.2%, African Ghana seed extract 0.3%, water 96.5%.Present invention process is simple, cheap, and equipment used in preparation process uses ozonization, to ensure water quality safety；Soda water cool taste returns sweet, alkalescent, and body sodium-potassium balance is adjusted；For sophora flower essence as sophora flower taste flavor regulator, mouthfeel is fresh and sweet, harmonious with soda water substrate and additive mouthfeel；The existing lemon in market, rose, minty taste are enriched, public consumption demand is met；The African Ghana's seed extract added in formula, main active are 5-hydroxyryptophan (5-HT), and pharmacological research shows that it is improved brain 5-HT concentration, inhibits melancholy, promote epiphysin synthesis, improve the effect of sleep quality.
Description
Technical field
The invention belongs to food processing technology fields more particularly to a kind of sophora flower taste soda water and preparation method thereof.
Background technique
With the rapid development of international commodity trade, soda water enters the country, becomes emerging beverage.Currently, domestic
The more well-known soda water brand in market is very few, and soda water is still within non-mainstream status, overall market also in exploitation,
Breeding phase, the developable market space are larger.Since soda water impression is novel, special taste is salubrious, the low burden of low energy
Feature, gradually captures beverage market, and sales volume rises year by year.But current soda water taste is single, mostly mint flavored, lemon
Taste, rose taste soda water；Effect additive is less, only finds a kind of Rosa roxburghii Tratt soda water of CN104256797A and its preparation at present
Method is added in carbonated water, the Rosa roxburghii Tratt taste soda water of preparation using Rosa roxburghi Juice；Patent (CN201510970416.0) discloses
A kind of Limon pulp soda water reaches lemon taste by the Limon pulp for adding fresh；Patent (CN107348278A) is public
A kind of corn soda water has been opened, has reached the taste of corn by adding sweet corn kernel, taste, which is difficult to meet, public increasingly to be increased
Long consumption demand.
Ghana's seed is the dry seed of tropical mesquite plant Ghana cereal (Griffoniasimplicifolia).From
Since Gu, the people in West Africa area just have using Ghana's seed as treatment disease history, such as using its leaf and its juice come
It treats wound and nephrosis, it is also possible to make enema and medea；Medicinal ointment therapy chancroid etc. is made into using the Ghana's bark smashed to pieces.
The study found that the main active substances of Ghana's seed are 5HTP, content can reach 10% or more.5HTP
Chemical structural formula it is as shown in Figure 1.Modern pharmacy is studies have shown that 5HTP also has multiple pharmacological effect, such as: adjusting
Mood treats depression, and it is aggressive to weaken patient；Shorten time for falling asleep, improves sleep quality；Weaken appetite, treatment is fat；It controls
Treat fibromyalgia syndrome；Alleviate chronic cephalalgia symptom etc..In addition, 5HTP is also obtained in medicine and health products trade
To being widely applied.
Although 5HTP content in Ghana's seed is up to 10% or so, it extracts isolated yield for a long time
Lower (60~70%).Also contain a large amount of 5HTP (Ghana's seed 5- hydroxyl in the mother liquor after separation the reason is that extracting
The 30~40% of tryptophan) product cannot be isolated.Since the document in relation to 5HTP preparation method is few, so far
Also it has no in the method for Ghana's seed extract crystalline mother solution industrialized production 5HTP monomer.
Prior art discloses a kind of methods that 5-hydroxyl tryptophan in high purity is prepared using Ghana's seed as raw material.This method uses
Supercritical extract and membrane separation technique make the purity of the 5HTP extracted reach 99% or more.Patent thinks: the party
Method has many advantages, such as simple process, with short production cycle, at low cost, is suitable for carrying out large-scale industrial production.However, above-mentioned special
Preparation method disclosed in benefit is using Ghana's seed as raw material, and the ult rec of product is 60~70%, in crystalline mother solution still
It is not separated containing larger amount of 5HTP so, the 5HTP that do not isolate accounts for entire 5- hydroxyl color ammonia
The 30~40% of acid.
5HTP is unstable under alkaline condition, though also having isolated product using ion exchange resin, produces
Product yield is lower；Meanwhile resin treatment, regeneration are extremely difficult to environmental requirement.
In conclusion main problem of the existing technology is:
Taste is single, effect is added still in preliminary development phase, and traded commodity repeatability is stronger, cannot effectively open up city
, meet public demand.
It cannot effectively be extracted in Ghana's seed crystalline mother solution containing 5HTP in the prior art, the extract benefit of acquisition
Low with rate, purity is low.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of sophora flower taste soda water and preparation method thereof.
Implementation of the present invention is as follows: a kind of sophora flower taste soda water, and the sophora flower taste soda water formula is according to quality
Percentage is made of sodium bicarbonate 3.0%, sophora flower essence 0.2%, African Ghana seed extract 0.3%, water 96.5%；
Another object of the present invention is to provide a kind of preparation method of sophora flower taste soda water, the sophora flower tastes
The preparation method of soda water the following steps are included:
Step 1: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot are cleaned with Ozone Water, disappeared
Poison, it is spare；
Step 2: ingredient: the sodium bicarbonate of certain mass and African Ghana seed extract are placed in material-compound tank and are added
Dissolved in purified water stirs evenly；
Step 3: by the solution stirred evenly filtering in finished pot, the sophora flower that corrresponding quality is added into finished pot is fragrant
Essence stirs evenly；
Step 4: into step 3 solution be added corrresponding quality pure water, be uniformly mixed to get.
Further, the extracting method of African Ghana's seed extract includes:
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/
ML), pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will
Concentrate merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: fresh lime cream and 10% sulphur acid for adjusting pH are separately added into the pre- product of Ghana's seed extract crystalline mother solution extremely
6.5~7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged, receive later
Collect filtrate, filter residue is air-dried, stores；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows:
70%~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to depressurize dense
It is reduced to dry, obtains mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, the ethyl alcohol with mass fraction 80~95% is water-soluble
Liquid washing and elution, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distillation
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by water recrystallization, until all returning the ethyl alcohol at rectifying without alcohol taste
It receives, obtains 5HTP.
Further, the dosage of silica gel described in step 6 is 1 times of Ghana's seed extract crystalline mother solution quality；
Further, it is detected when collecting eluent in step 7 using thin-layer chromatography；
Ethanol water is collected during the reduced pressure.
Further, the dosage of silica gel is 0.5 times of mother liquor spice quality in the chromatographic column.
Further, silica regeneration described in step 7 uses silica gel activating furnace；
Filtering is using vacuum filtration or centrifuge rejection filter.
Further, the solvent of the thin-layer chromatography detection is ethanol water, and color developing agent is ethanol solution of ninhydrin.
Further, in step 2, extracting in water material liquid ratio is 1:80 (g/mL)~1:90 (g/mL).
Further, in step 2, extracting in water object extraction temperature is 70-80 DEG C, extraction time 90-120min
In conclusion advantages of the present invention and good effect are as follows:
Sophora flower taste soda water of the present invention and preparation method thereof, preparation process is simple, cheap, then institute in preparation process
Equipment all carries out ozonization, it is ensured that water quality it is pure with it is safe, soda water is alkalescent water, effectively maintain body sodium
Potassium balance is added to African Ghana's seed extract in formula, living in the biology of African Ghana's seed ethanol extract of Zheng Zhizhong
It is mentioned in one text of Journal of Sex Research, the main active in African Ghana's seed extract is 5HTP, can improve brain 5-
HT concentration inhibits melancholy, promotes epiphysin synthesis, improves the effect of sleep quality, and the present invention is added to sophora flower essence, makes
The mouthfeel of soda water is fresh and sweet, harmonious with soda water substrate and additive mouthfeel, and it is single to have broken soda water taste in the market
Situation, the consumption demand of compound masses.
In the extracting method of Africa Ghana provided by the invention seed extract,
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/
ML), pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will
Concentrate merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: fresh lime cream and 10% sulphur acid for adjusting pH are separately added into the pre- product of Ghana's seed extract crystalline mother solution extremely
6.5~7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged, receive later
Collect filtrate, filter residue is air-dried, stores；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows:
70%~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to depressurize dense
It is reduced to dry, obtains mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, the ethyl alcohol with mass fraction 80~95% is water-soluble
Liquid washing and elution, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distillation
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by water recrystallization, until all returning the ethyl alcohol at rectifying without alcohol taste
It receives, obtains 5HTP.
Mother liquor spice is mixed to obtain with Ghana seed extract crystalline mother solution using silica gel, can solve 5- hydroxyl during elution
The problem of dissolubility difference of the tryptophan in high concentration ethanol solution.
Be packed into silica gel in chromatographic column and be used as adsorbent, influence in adsorbable mother liquor spice 5HTP crystallize it is miscellaneous
Matter effectively removes impurity, and the eluent of collection is easy to crystallize, and the product 5HTP purity of crystallization is up to 99.5%
More than.
The eluent collected after the elution of mother liquor spice and washing collects ethanol water weight using reduced pressure crystallization
It is multiple to use, it is recycled cost-saved and more environmentally-friendly.
Silica gel activating furnace activating and regenerating can be used in the silica gel of chromatographic column, and silica gel is reusable, more safety and environmental protection.This
Invention solves the problems, such as to separate 5HTP, the 5- hydroxyl color ammonia of extraction from Ghana's seed extract crystalline mother solution for the first time
The purity of acid product is up to 99.5% or more；This simple and convenient extraction, adsorbent and eluant, eluent are reusable, and solvent uses
Measure small, production cost is low, is suitable for industrialization large-scale production.
Processing to the pre- product of Ghana's seed extract crystalline mother solution ensure that the extract of acquisition has the guarantor of higher degree
Card.
Detailed description of the invention
Fig. 1 is the flow chart of the preparation method of sophora flower taste soda water provided in an embodiment of the present invention.
Specific embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and cooperate attached drawing
Detailed description are as follows.
Structure of the invention is explained in detail with reference to the accompanying drawing.
Sophora flower taste soda water provided in an embodiment of the present invention is added by sodium bicarbonate 3.0%, sophora flower essence 0.2%, Africa
Receive seed extract 0.3%, water 96.5% form.
As shown in Figure 1, the preparation method of sophora flower taste soda water provided in an embodiment of the present invention the following steps are included:
S101: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot being cleaned with Ozone Water, sterilized,
It is spare；
S102: ingredient: the sodium bicarbonate of certain mass and African Ghana's seed extract are placed in material-compound tank be added it is pure
Water purification dissolution, stirs evenly；
S103: by the solution stirred evenly filtering in finished pot, the sophora flower essence of corrresponding quality being added into finished pot,
It stirs evenly；
S104: into S103 solution be added corrresponding quality pure water, be uniformly mixed to get.
The invention will be further described combined with specific embodiments below.
The molecular structural formula of 5HTP of the present invention are as follows:
Embodiment 1,
The extracting method of Africa Ghana seed extract provided in an embodiment of the present invention includes:
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/
ML), pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will
Concentrate merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: fresh lime cream and 10% sulphur acid for adjusting pH are separately added into the pre- product of Ghana's seed extract crystalline mother solution extremely
6.5~7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged, receive later
Collect filtrate, filter residue is air-dried, stores；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows:
70%~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to depressurize dense
It is reduced to dry, obtains mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, the ethyl alcohol with mass fraction 80~95% is water-soluble
Liquid washing and elution, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distillation
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by water recrystallization, until all returning the ethyl alcohol at rectifying without alcohol taste
It receives, obtains 5HTP.
Wherein, take it is above-mentioned be concentrated under reduced pressure into thick Ghana seed crystalline mother solution 5kg, 5kg silica gel is added, mixes, decompression
Concentration is done to obtain mother liquor spice, and by mother liquor spice, by the chromatographic column progress column chromatography equipped with silica gel, (silica gel is 100~200
Purpose silochrom, chromatographic column specification are that 5kg silica gel is previously charged into chromatographic column, and mother liquor spice is added in above silica gel), use second
Alcohol solution (mass fraction is 80~95%) washing and elution, the eluent of quantitative collection, collection are detected using thin-layer chromatography
(solvent is ethanol water (ethyl alcohol: water=100:9 (volume ratio)), and color developing agent is the triketohydrindene hydrate ethyl alcohol of mass fraction 2%
Liquid, heating colour developing), it collects and merges qualified eluent, eluent is concentrated under reduced pressure and recycles ethanol water (ethyl alcohol of recycling is water-soluble
It is reusable after liquid adjustment ratio), crystallization, vacuum filtration are placed, crystal distilled water recrystallizes once up to 5- hydroxyl color
Propylhomoserin monomer 310g, yield 6.2%, 5HTP content 99.5% in product.
Embodiment 2:
The extracting method of Africa Ghana seed extract provided in an embodiment of the present invention includes:
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/
ML), pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will
Concentrate merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: fresh lime cream and 10% sulphur acid for adjusting pH are separately added into the pre- product of Ghana's seed extract crystalline mother solution extremely
6.5~7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged, receive later
Collect filtrate, filter residue is air-dried, stores；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows:
70%~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to depressurize dense
It is reduced to dry, obtains mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, the ethyl alcohol with mass fraction 80~95% is water-soluble
Liquid washing and elution, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distillation
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by water recrystallization, until all returning the ethyl alcohol at rectifying without alcohol taste
It receives, obtains 5HTP.
Wherein, it is concentrated under reduced pressure into thick Ghana seed crystalline mother solution 10kg in Example 1,10kg silica gel is added, mixes
It is even, be concentrated under reduced pressure it is dry to obtain mother liquor spice, mother liquor spice by the chromatographic column equipped with silica gel is carried out column chromatography, and (silica gel is
The silochrom of 100~200 mesh, chromatographic column specification are that 10kg silica gel is previously charged into chromatographic column, and mother liquor spice adds on silica gel
Face), it is washed with ethanol water (mass fraction is 80~95%) and uses thin layer with elution, the eluent of quantitative collection, collection
It is ethanol water (ethyl alcohol: water=100:9 (volume ratio)) that chromatography, which detects solvent, and color developing agent is the print three of mass fraction 2%
Ketone ethanol, heating colour developing) the qualified eluent of merging is collected, eluent is concentrated under reduced pressure and recycles the ethanol water (second of recycling
It is reusable after alcohol solution adjustment ratio), crystallization, vacuum filtration are placed, crystal distilled water recrystallizes once up to 5-
Hydroxytryptophan monomer 650g, yield 6.5%, 5HTP content 99.8% in product.
Embodiment 3:
The extracting method of Africa Ghana seed extract provided in an embodiment of the present invention includes:
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/
ML), pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will
Concentrate merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: fresh lime cream and 10% sulphur acid for adjusting pH are separately added into the pre- product of Ghana's seed extract crystalline mother solution extremely
6.5~7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged, receive later
Collect filtrate, filter residue is air-dried, stores；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows:
70%~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to depressurize dense
It is reduced to dry, obtains mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, the ethyl alcohol with mass fraction 80~95% is water-soluble
Liquid washing and elution, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distillation
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by water recrystallization, until all returning the ethyl alcohol at rectifying without alcohol taste
It receives, obtains 5HTP.
Wherein,
It is concentrated under reduced pressure into thick Ghana seed crystalline mother solution 50kg in Example 1,50kg silica gel is added, mixes, subtracts
Pressure concentration is dry to obtain mother liquor spice, by mother liquor spice by the chromatographic column equipped with silica gel carry out column chromatography (silica gel is 100~
The silochrom of 200 mesh, chromatographic column specification are that 50kg silica gel is previously charged into column, and mother liquor spice is added in above silica gel), use second
Alcohol solution washing and elution, quantitative collection, the eluent of collection use thin-layer chromatography detection solvent for ethanol water (second
Alcohol: water=100:9 (volume ratio)), triketohydrindene hydrate ethanol of the color developing agent for mass fraction 2%, heating colour developing) collect merging qualification
Eluent is concentrated under reduced pressure eluent and recycles ethanol water (reusable after the ethanol water adjustment ratio of recycling),
Crystallization, vacuum filtration or centrifuge rejection filter are placed, crystal distilled water recrystallizes once up to 5HTP monomer
3150g, yield 6.3%, 5HTP content 99.7% in product.
The above is only the preferred embodiments of the present invention, and is not intended to limit the present invention in any form,
Any simple modification made to the above embodiment according to the technical essence of the invention, equivalent variations and modification, belong to
In the range of technical solution of the present invention.
Claims (10)
1. a kind of sophora flower taste soda water, which is characterized in that the sophora flower taste soda water is according to mass percent by bicarbonate
Sodium 3.0%, sophora flower essence 0.2%, African Ghana seed extract 0.3% and water 96.5% form.
2. a kind of preparation method of sophora flower taste soda water as described in claim 1, which is characterized in that the sophora flower taste soda
The preparation method of water the following steps are included:
Step 1: equipment cleaning: the material-compound tank used in preparation process, pipeline, finished pot being cleaned with Ozone Water, sterilized, standby
With；
Step 2: ingredient: the sodium bicarbonate of certain mass and African Ghana's seed extract are placed in material-compound tank be added it is pure
Water dissolution, stirs evenly；
Step 3: by the solution stirred evenly filtering in finished pot, the sophora flower essence of corrresponding quality is added into finished pot, stirs
It mixes uniformly；
Step 4: into step 3 solution be added corrresponding quality pure water, be uniformly mixed to get.
3. the preparation method of sophora flower taste soda water as described in claim 1, which is characterized in that African Ghana's seed extract mentions
The method is taken to include:
Step 1, pretreatment of raw material, Ghana's seed are selected, are cleaned, and drying to constant weight, are crushed, and 60 meshes are crossed；
Step 2, smashed Ghana's seed is accurately weighed, distilled water is added, material liquid ratio is 1:70 (g/mL)~1:90 (g/mL),
Pan-fried 30~80min is boiled, upper layer filtrate is taken, is concentrated into material liquid ratio are as follows: 1:30 (g/mL) is repeated above operation three times, will be concentrated
Liquid merges；Obtain the pre- product of Ghana's seed extract crystalline mother solution；
Step 3: be separately added into the pre- product of Ghana's seed extract crystalline mother solution fresh lime cream and 10% sulphur acid for adjusting pH to 6.5~
7.0；
Step 4；Excessive ethyl alcohol will be added in filter residue filtered in step 2 impregnate 36 hours, be centrifuged later, collect filter
Liquid air-dries filter residue, storage；
Step 5: when the pre- product temperature degree of Ghana's seed extract crystalline mother solution is cooled to 40 DEG C, addition ethyl alcohol makes ethanol content are as follows: 70%
~85%, it is concentrated under reduced pressure and elutes, obtain thick Ghana's seed extract crystalline mother solution；
Step 6: to being concentrated under reduced pressure into thick Ghana's seed extract crystalline mother solution, silica gel is added, continues to be concentrated under reduced pressure into
It is dry, obtain mother liquor spice；
Step 7: mother liquor spice being added in the chromatographic column equipped with silica gel, with the ethanol aqueous wash of mass fraction 80~95%
It washs and elutes, collect eluent, after the eluent of collection is concentrated under reduced pressure, place crystallization, filtered crystal distilled water weight
Recrystallization alcohol extract is carried out multiple rectifying by rectifying column by crystallization, until all recycling, obtaining by the ethyl alcohol at rectifying without alcohol taste
5HTP.
4. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that the use of silica gel described in step 6
Amount is 1 times of Ghana's seed extract crystalline mother solution quality.
5. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that when collecting eluent in step 7
It is detected using thin-layer chromatography；
Ethanol water is collected during the reduced pressure.
6. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that the use of silica gel in the chromatographic column
Amount is 0.5 times of mother liquor spice quality.
7. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that silica gel described in step 7 is again
It is raw to use silica gel activating furnace；
Filtering is using vacuum filtration or centrifuge rejection filter.
8. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that the exhibition of the thin-layer chromatography detection
Opening agent is ethanol water, and color developing agent is ethanol solution of ninhydrin.
9. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that in step 2, extracting in water material
Liquor ratio is 1:80 (g/mL)~1:90 (g/mL).
10. the preparation method of sophora flower taste soda water as claimed in claim 3, which is characterized in that in step 2, extracting in water object
Extraction temperature is 70-80 DEG C, extraction time 90-120min.
Priority Applications (1)
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