CN109349222A - A kind of method that molecule auxiliary cultivates short-foot layer of green-shell egg strain - Google Patents
A kind of method that molecule auxiliary cultivates short-foot layer of green-shell egg strain Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The present invention discloses a kind of method that molecule auxiliary cultivates short-foot layer of green-shell egg strain, belongs to poultry breeding technical field.Specific method is to hybridize short-foot strain and layer of green-shell egg strain；Combine and identified using different molecular labelings, retain F1 generation green-egg-shelled heterozygote individual, while carrying short-foot hen homozygote or short-foot cock heterozygote individual, and carries out verifying according to short-foot and green-egg-shelled phenotype and eliminate again；The F1 male and female chicken giblets zygote reserved seed for planting carries out traversed by；It is screened using molecular labeling, retain F2 generation while carrying the individual of green-egg-shelled homozygote genotype and short-foot homozygote genotype, identified and eliminated further according to short-foot and green-egg-shelled phenotype, finally obtain purebred short-foot layer of green-shell egg strain.
Description
Technical field
The present invention relates to poultry breeding technical fields, and in particular to a kind of molecule auxiliary cultivates short-foot layer of green-shell egg strain
The method of method and the cultivate effect verifying of method.
Background technique
In recent years, domestic and international nutritionist and physician summarize the nutritive value of egg and healthcare function mainly includes brain tonic
Five broad aspects such as intelligence development, protection liver, prevention and treatment artery sclerosis, pre- anti-cancer, anti-aging.Green-egg-shelled is on above functions basis
On, because it contains the essential vitamin and mineral of a large amount of human bodies and has high biological value protein, repairing human body group
Knit, form new tissue, consumption energy and participate in complicated metabolic processes etc. plays an important role.It is short and small
Chicken has advantages such as basal metabolic rate is low, feed consumption is few, laying rate is high, stocking density is big, adaptability and resisting stress are strong and its obviously
Economic benefit.Therefore, short-foot layer of green-shell egg not only increases the egg laying performance of chicken on the basis of green-egg-shelled, and reduces
Food consumption, to have the bigger market demand and economic benefit and be widely used in contemporary domestic fowl farming production.
Using traditional selection, a large amount of livestock and poultry species have successfully been cultivated both at home and abroad.But traditional breeding method
Mainly offspring is selected according to phenotype by experience in breeding, the genotype of target gene can not be directly selected.Cause
This, efficiency of selection and accuracy are lower.And utilize molecular labeling can from DNA level Direct Identification individual genotype, avoid
Phenotype speculates the disadvantages of genotype is inaccurate.Either to qualitative character or quantitative quantitative character, molecule is utilized
Marker assisted selection is remarkably improved efficiency of selection and accuracy, accelerates breeding process.Specifically, molecular marker assisted selection
With the difficulty for overcoming trait phenotypes to identify, can be in growth and development Seedling selection, multiple (equipotential) genes of the same character of control
Utilization, allowing the multiple characters of simultaneous selection, Character Evaluation and selection not to have, destructive, back cross breeding efficiency to can be improved etc. excellent
More property.
Sex-linkage dwarf gene dw (dwarf) is the main effect base of numerous quantitative characters such as weight, the price of deed, egg laying performance
Cause, the double inheritance with qualitative character and quantitative character are incomplete Recessive alleles.Chicken growth hormone receptor
(GHR) genetic mutation leads to chain short and small chicken, since dwarf chicken lacks growth hormone receptor, influences the biological function of growth hormone
Can, to prevent GH dependent gene from normal expression.The variation of different chicken kinds is different, and having now been found that can there are three reason
Lead to the short and small of chicken: firstly, growth hormone receptor (GHR) gene 1.7kb fragment deletion causes；Second, GHR gene extron
T354C mutation causes short and small；Third, due to the variation in a unknown site cause it is short and small.It finds after study, Nanjing Bantam
The origin cause of formation be since GHR gene 1.7kb fragment deletion causes.
Egg shell color is important egg traits, has been a concern.The green shell character of chicken is dominant on No. 1 chromosome
The result of oocyan gene (Oocyan, O) effect.The oocyan gene (O) of chicken is positioned on No. 1 the short arm of a chromosome, it and endogenous disease
The malicious factor (ev1) and pea comb site (P) are chain, their putting in order as P-evl-O on chromosome.Further study show that
The green shell character of chicken is to transport polypeptide 1B3 gene (Solute carrier organic an-ion by organic anion
Transporter family member 1B3, SLCO1B3 are located at No. 1 65319441~65338450bp of chromosome) 5 ' sides
Pterion is inserted into caused by birds retrovirus (EAV-HP, length 4.2kb).When EAV-HP is inserted into the 5 ' of SLCO1B3
After flanking region, cause SLCO1B3 gene in uterine part specifically expressing, so that eggshell color be made to show as green shell.
Summary of the invention
The object of the present invention is to provide a kind of fast breeding method of short-foot layer of green-shell egg, which utilizes molecule mark
Remember that assistant breeding technology breaches the routine phenotypic breeding disadvantage that time-consuming, purity is low using the Breeding strategy of gene pyramiding,
It can be applied to the rapid and simple cultivation for carrying the short-foot layer of green-shell egg strain of short-foot and green-egg-shelled performance simultaneously, and related strain
Performance study and production.Purpose to realize the present invention, used technical solution are as follows:
A kind of method that molecule auxiliary cultivates short-foot layer of green-shell egg strain, comprising the following steps:
(1) selection is well-proportioned, active health, weight reach average weight or more, and reach sexually matured cock and mother
Chicken is as parent to be selected, after being hybridized in a manner of mixed essence, collects hatching egg and hatches, obtain F1 generation young bird；The cock phenotype
For yellow plumage, white skin, short-foot, powder shell egg；The hen phenotype is black plumage, white skin, high foot, green-egg-shelled；
(2) F1 generation young bird is cultivated to 3-4 week old, acquire blood and extracts genomic DNA, use GHR and SLCO1B3 gene
Molecular labeling PCR detection is carried out to F1 generation young bird, retain and carry the sub- genotype of SLCO1B3 genetic heterozygosis, while carrying GHR base
Because of the cock individual of heterozygote genotype and the hen individual of GHR genetic homozygous；
The molecular labeling primer sequence of the GHR gene is as shown in SEQ ID NO.1~SEQ ID NO.4；
The molecular labeling primer sequence of the SLCO1B3 gene is as shown in SEQ ID NO.5~SEQ ID NO.7；
(3) individual for filtering out step (2) is cultivated to 9-14 week old, when hen egg-laying peak, passes through foot shank length and egg
The verifying molecule screening of shell color phenotype is as a result, retain the individual for being provided simultaneously with short-foot and green-egg-shelled；
(4) by the cock of step (3) screening and hen, traversed by, collection hatching egg are simultaneously hatched in a manner of mixed essence, obtain F2 for young bird；
(5) F2 is cultivated for young bird to 3-4 week old, acquire blood and extracts genomic DNA, use GHR and SLCO1B3 gene
Molecular labeling to F2 for young bird carry out PCR detection, retain at the same carry GHR homozygote genotype and SLCO1B3 homozygosis subbase
Because of the individual of type；
The molecular labeling primer sequence of the GHR gene is as shown in SEQ ID NO.1~SEQ ID NO.4；
The molecular labeling primer sequence of the SLCO1B3 gene is as shown in SEQ ID NO.5~SEQ ID NO.7；
(6) F2 is cultivated for young bird to 9-14 week old, hen egg-laying peak, is verified by foot shank length and eggshell color phenotype
Molecule screening is as a result, retain the individual for being provided simultaneously with short-foot and green-egg-shelled.
Preferably, the step (3) and (6) foot shank length≤6cm are short-foot.
Preferably, the PCR reaction condition of the step (2) and (5) is 20.0 μ L reaction systems, including 1.0U Taq DNA
Polymerase, 1.0 10 × buffer of μ L, 50ng template DNA, each 3.0pmol/L of primer, each 2.0 μm of ol/L of 4 kinds of dNTP；PCR is anti-
Answering program is 94 DEG C of initial denaturation 5min；95 DEG C of 30s, 58 DEG C of annealing temperatures 30s, 72 DEG C of 30s amount to 36 circulations；72 DEG C of extensions
5min。
Preferably, the GHR gene criterion are as follows: display 417bp band be short-foot homozygote, display 417bp and
217bp band person is short-foot heterozygote.
Preferably, the SLCO1B3 gene criterion are as follows: display 425bp band person is green shell homozygote, display display
425bp and 340bp band person is green shell heterozygote.
The present invention also provides a kind of molecule auxiliary as previously discussed to cultivate the short of the method cultivation of short-foot layer of green-shell egg strain
Foot layer of green-shell egg.
The method of the invention includes: that short-foot strain and layer of green-shell egg strain are hybridized；Joint utilizes different molecules
Label is identified, retains F1 generation green-egg-shelled heterozygote individual, while carrying short hen foot homozygote or short-foot cock heterozygote
Individual, and carry out verifying according to short-foot and green-egg-shelled phenotype and eliminate again；The F1 male and female chicken giblets zygote reserved seed for planting carries out traversed by；Benefit
It is screened with molecular labeling, retain F2 generation while carrying of green-egg-shelled homozygote genotype and short-foot homozygote genotype
Body, is identified and is eliminated again according to short-foot and green-egg-shelled phenotype, and purebred short-foot layer of green-shell egg strain is finally obtained.
The green-egg-shelled strain hen that the present invention uses is green-egg-shelled heterozygote or pure lines individual, it is preferable that the green shell used
Egg product system hen is green-egg-shelled pure lines individual, is that the non-layer of green-shell egg individual reduced occurs compared to the advantage of heterozygote,
Reduce feeding cost and artificial waste.
Acquisition blood simultaneously extracts genomic DNA (referring to " Molecular Cloning:A Laboratory guide IV ", following DNA extracts identical), first
Joint identification is carried out using the molecular labeling of short-foot and green-egg-shelled trait related gene afterwards；In 3-4 weeks progress molecular labeling joint
Identification, by carrying out detection in advance, it is possible to reduce intermediate raising expense, it is contemplated that the acquisition of mesoderm growing early stage blood may will affect
Remember to develop, therefore is further preferably 4 week old.It is of the present invention to extract containing DNA referring to " Molecular Cloning:A Laboratory guide
IV ", agents useful for same is conventional reagent, is purchased from Reagent Company；
Retain while carrying green-egg-shelled homozygous genotype and short-foot heterozygote genotype cock individual in step (2) or short-foot is homozygous
Retain while carrying green-egg-shelled and short-foot homozygous genotype individual in sub- genotype hen individual and step (5), is according to Meng De
You determine genetic development.
In 9-14 week old, hen egg-laying peak in step (3) and (6), respectively according to foot shank length (9-14 week old) or
Eggshell color (hen egg-laying peak) isophenous verifies molecular marker screening as a result, eliminating phenotype undesirable simultaneously
Body；It wherein, is within 9-14 weeks that the chicken feet shin development completion phase with the increase of week old can be further improved the accurate of observed result
Degree, but extend observation week old again and will lead to intermediate raising expense increase, therefore be further preferably 14 week old；Egg-laying peak
It is individual amount most period of laying eggs in group, therefore this period obtains best observed result；In molecular markers for identification
On the basis of carry out phenotype verifying and eliminate, it is ensured that and improve qualification result accuracy；
Gene pyramiding strategy of the present invention refers to the molecule that short-foot related gene and green-egg-shelled related gene is used in combination
Label is screened respectively, the pure lines finally obtained while having short-foot and green-egg-shelled feature.
The present invention also provides the above methods in the short-foot layer of green-shell egg product for being directed to while carrying short-foot and green-egg-shelled performance
The rapid and simple cultivation of system, and related strain production etc. application.
GHR and SLCO1B3 is used in combination using molecular mark technology using gene pyramiding strategy in the present invention
The molecular marker screening of gene judges in conjunction with phenotype, breaches the routine phenotypic breeding disadvantage that time-consuming, purity is low, provide
A kind of fast breeding method of short-foot layer of green-shell egg.It is green present invention can apply to carry the short-foot of short-foot and green-egg-shelled performance simultaneously
The rapid and simple cultivation of shell layer chicken strain, and performance study and the production of related strain；And it is directed to short-foot and green-egg-shelled character
The joint identification method of molecular labeling is provided, and the purity of the short-foot layer of green-shell egg strain obtained verifies analysis.
Traditional poultry breeding results in efficiency of selection and accurate because can not directly select to the genotype of target gene
Property it is lower, and molecular marker assisted selection is remarkably improved efficiency of selection and accuracy, accelerates breeding process.
The present invention is successfully realized using gene pyramiding strategy with multiple excellent using Molecular Marker Assisted Selection Technology
The quick breeding of the chicken new lines of benign shape (short-foot and green-egg-shelled).Present invention can apply to carry short-foot and green-egg-shelled simultaneously
Can short-foot layer of green-shell egg strain rapid and simple cultivation, and performance study and the production etc. of related strain.
The present invention has the characteristics that breeding process is fast, breeding accuracy is high；Meanwhile the present invention because its early stage can shift to an earlier date
Carry out molecular marker screening, so having many advantages, such as that intermediate raising expense can be saved.
Detailed description of the invention
Fig. 1 is the short-foot layer of green-shell egg pure lines picture of 1 Markers for Detection agarose gel electrophoresis of embodiment and acquisition；Its
In
A:F1 is for agarose gel electrophoresis figure after the molecular labeling PCR amplification of short-foot trait related gene GHR；1. wild-type homozygous
Son, 2. heterozygotes；
B:F1 is for agarose gel electrophoresis figure after the molecular labeling PCR amplification of green-egg-shelled trait related gene SLCO1B3；1. wild
Type homozygote, 2. heterozygotes；
C:F2 is for agarose gel electrophoresis figure after the molecular labeling PCR amplification of short-foot trait related gene GHR；1. wild-type homozygous
Son, 2. heterozygotes, 3. no mutant homozygote；
D:F2 is for agarose gel electrophoresis figure after the molecular labeling PCR amplification of green-egg-shelled trait related gene SLCO1B3；1. wild
Type homozygote, 2. heterozygotes, 3. no mutant homozygote；
E: the short-foot layer of green-shell egg of acquisition is sheerly picture.
Fig. 2 is the molecular labeling of the short-foot for the short-foot layer of green-shell egg pure lines that embodiment 2 obtains and the purity of green-egg-shelled performance
Detect agarose gel electrophoresis figure；Wherein
A: agarose gel electrophoresis figure after the molecular labeling PCR amplification of pure generation offspring individuals short-foot trait related gene GHR；1-4.
No mutant homozygote；
B: agarose gel electrophoresis after the molecular labeling PCR amplification of pure generation offspring individuals green-egg-shelled trait related gene SLCO1B3
Scheme, B.1-4. no mutant homozygote.
Specific embodiment
The present invention program is described in further detail below with reference to embodiment, following the description is merely to explain this hair
It is bright, its content is not defined.Experimental method in following embodiments is unless otherwise specified conventional method.It is following
Test material used in embodiment and reagent are unless otherwise specified conventional biochemical reagent, are purchased from Reagent Company.
The cultivation and acquisition of 1 short-foot layer of green-shell egg of embodiment pure lines
The heterozygosis primary and secondary for the new poplar layer of green-shell egg (black plumage, white skin, high foot, green-egg-shelled) introduced a fine variety from Xinyan Fowl Breeding Centre, Shanghai
Chicken 7500, select the hen individual that 1000 appearance, figures are all good, the gold having by oneself with 80 Guangxi Nanjing agriculture and animal husbandry group company
Mound short-foot strain cock (Huang Yu, white skin, short-foot, powder shell egg) is hybridized in a manner of mixed essence, collects hatching egg, hatching, is obtained
10040 F1 generation young birds.
Eliminate that appearance is unqualified, lopsided and unhealthy individual in breeding process at any time, F1 generation acquires blood when raising to 4 week old
Liquid simultaneously extracts DNA, and the molecular labeling that GHR and SLCO1B3 gene is used in combination respectively carries out short-foot and green-egg-shelled character purity
Detection, retain carry the sub- genotype of SLCO1B3 genetic heterozygosis, while carry the sub- genotype of GHR genetic heterozygosis cock individual and
The hen individual of GHR gene pure body.The primer information and PCR program of the molecular labeling of GHR and SLCO1B3 are shown in Table 1.Raising is extremely
Non- short-foot individual is eliminated when 14 week old, retains foot shank length≤6cm short-foot individual；Continue raising to F1 generation hen to lay eggs peak
Phase carries out superseded according to the hen individual that eggshell color produces powder shell egg to F1 generation.Finally identify satisfactory cock 130
Only, hen 1000.
Markers for Detection primer information, PCR response procedures and genotype criterion used in 1 present invention of table
Satisfactory 130 cocks, 1000 hens carry out traversed by a manner of mixed essence.Hatching egg, hatching are collected, F2 generation is obtained
Young bird 13000.Entire raising and screening process and operate same F1 generation, breeding goal for and meanwhile carry short-foot, the public affairs of green-egg-shelled,
Hen homozygotic individual, final obtain includes cock 27, and hen 345 short-foot layer of green-shell egg are sheerly (black plumage, white skin).
The result shows that molecular markers for identification is that hen is entirely short-foot homozygous genotype individual, public in first familiar generation individual
Chicken is then that heterozygous genotypes are individual (Figure 1A), and all cocks and hen are powder shell egg homozygous genotype and green-egg-shelled heterozygous genes
Type individual (Figure 1B)；In the F1 offspring for meeting requirement of reserving seed for planting retained after Molecular Identification, cock is high foot, and hen is equal
For short-foot, and the eggshell color that hen is laid eggs is layer of green-shell egg.These results show that molecular labeling of the present invention
Accuracy rate and applicability.
In traversed by F2 generation individual, molecular markers for identification is that hen is entirely short-foot homozygous genotype individual, cock and hen
In there are heterozygous genotypes and 2 kinds of homozygous genotype (Fig. 1 C), there are powder shell egg homozygous genotype, green-egg-shelled homozygous gene with
3 kinds of green-egg-shelled heterozygous genotypes (Fig. 1 D)；In the F2 offspring for meeting requirement of reserving seed for planting retained after Molecular Identification, donor stem cell infusion
It is short-foot, and the eggshell color that hen is laid eggs is layer of green-shell egg.Result above complies fully with mendelian inheritance, also again
The secondary accuracy rate and applicability for confirming molecular labeling of the present invention, while illustrating to utilize short-foot and green shell by joint
The pure lines (Fig. 1 E) of short-foot layer of green-shell egg have successfully been obtained in the molecular labeling of egg related gene.
The purity check of the short-foot and green-egg-shelled performance of the short-foot layer of green-shell egg pure lines that embodiment 2 obtains
The short-foot layer of green-shell egg pure lines that embodiment 1 is obtained carry out traversed by, and obtain young bird at the beginning of pure lines F3 offspring 2000.All
Body acquires blood when 4 week old, identifies the genotype purity of short-foot and green-egg-shelled character after extraction DNA on molecular level；Raising
To 14 week old, the foot shank length of identification pure lines offspring；The eggshell face that raising is laid eggs to egg-laying peak, identification pure lines offspring hen
Color.
As a result, it has been found that (as shown in table 2), on a molecular scale, the gene of short-foot and green-egg-shelled character that when 4 week old is identified
Type is homozygous (Fig. 2A and 2B)；When raising to 14 week old, 1931 donor stem cell infusion individuals of survival all carry short-foot
Shape；Equally, when raising is to egg-laying peak, in 891 hens individual for laying eggs, the eggshell color of produced egg is green
Shell.By the above phenotype and molecular level qualification result, illustrates and confirm that method of the invention obtains short-foot layer of green-shell egg
The pure lines of strain.
The short-foot and green-egg-shelled performance purity detecting data for the short-foot layer of green-shell egg pure lines that table 2 obtains
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, it is any without
The change or replacement that creative work is expected are crossed, should be covered by the protection scope of the present invention.Therefore, protection of the invention
Range should be determined by the scope of protection defined in the claims.
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atgttagcag tgtagttg 18
<210> 7
<211> 19
<212> DNA
<213>artificial sequence (Artificial sequence Latin)
<400> 7
taggttccga acgcgatgt 19
Claims (6)
1. a kind of method that molecule auxiliary cultivates short-foot layer of green-shell egg strain, which comprises the following steps:
(1) selection is well-proportioned, active health, weight reach average weight or more, and reach sexually matured cock and mother
Chicken is as parent to be selected, after being hybridized in a manner of mixed essence, collects hatching egg and hatches, obtain F1 generation young bird；The cock phenotype
For yellow plumage, white skin, short-foot, powder shell egg；The hen phenotype is black plumage, white skin, high foot, green-egg-shelled；
(2) F1 generation young bird is cultivated to 3-4 week old, acquire blood and extracts genomic DNA, use GHR and SLCO1B3 gene
Molecular labeling PCR detection is carried out to F1 generation young bird, retain and carry the sub- genotype of SLCO1B3 genetic heterozygosis, while carrying GHR base
Because of the cock individual of heterozygote genotype and the hen individual of GHR genetic homozygous；
The molecular labeling primer sequence of the GHR gene is as shown in SEQ ID NO.1~SEQ ID NO.4；
The molecular labeling primer sequence of the SLCO1B3 gene is as shown in SEQ ID NO.5~SEQ ID NO.7；
(3) individual for filtering out step (2) is cultivated to 9-14 week old, when hen egg-laying peak, passes through foot shank length and egg
The verifying molecule screening of shell color phenotype is as a result, retain the individual for being provided simultaneously with short-foot and green-egg-shelled；
(4) by the cock of step (3) screening and hen, traversed by, collection hatching egg are simultaneously hatched in a manner of mixed essence, obtain F2 for young bird；
(5) F2 is cultivated for young bird to 3-4 week old, acquire blood and extracts genomic DNA, use GHR and SLCO1B3 gene
Molecular labeling to F2 for young bird carry out PCR detection, retain at the same carry GHR homozygote genotype and SLCO1B3 homozygosis subbase
Because of the individual of type；
The molecular labeling primer sequence of the GHR gene is as shown in SEQ ID NO.1~SEQ ID NO.4；
The molecular labeling primer sequence of the SLCO1B3 gene is as shown in SEQ ID NO.5~SEQ ID NO.7；
(6) F2 is cultivated for young bird to 9-14 week old, hen egg-laying peak, is verified by foot shank length and eggshell color phenotype
Molecule screening is as a result, retain the individual for being provided simultaneously with short-foot and green-egg-shelled.
2. the method that molecule auxiliary cultivates short-foot layer of green-shell egg strain according to claim 1, which is characterized in that the step
(3) and (6) foot shank length≤6cm is short-foot.
3. the method that molecule auxiliary cultivates short-foot layer of green-shell egg strain according to claim 1, which is characterized in that the step
(2) and the PCR reaction condition of (5) is 20.0 μ L reaction systems, including 1.0 U Taq archaeal dna polymerases, 1.0 μ L 10 ×
Buffer, 50 ng template DNAs, each 3.0 pmol/L of primer, each 2.0 μm of ol/L of 4 kinds of dNTP；PCR response procedures are 94 DEG C
5 min of initial denaturation；95 DEG C of 30 s, 58 DEG C of annealing temperatures 30 s, 72 DEG C of 30 s amount to 36 circulations； 72 ℃
Extend 5 min.
4. the method that molecule auxiliary cultivates short-foot layer of green-shell egg strain according to claim 1, which is characterized in that the GHR
Gene criterion are as follows: display 417bp band is short-foot homozygote, shows 417 bp and 217 bp band persons are short-foot heterozygosis
Son.
5. the method that molecule auxiliary cultivates short-foot layer of green-shell egg strain according to claim 1, which is characterized in that described
SLCO1B3 gene criterion are as follows: 425 bp band persons of display are green shell homozygote, display 425 bp of display and 340 bp items
Band person is green shell heterozygote.
6. a kind of short-foot green-egg-shelled that the method that molecule auxiliary as described in claim 1 cultivates short-foot layer of green-shell egg strain is cultivated
Chicken.
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| CN111213614A (en) * | 2019-04-11 | 2020-06-02 | 广西大学 | Method for molecular marker-assisted cultivation of fishy smell-free green-leg pockmarked-feather green-shell laying hens |
| CN111213613A (en) * | 2019-04-29 | 2020-06-02 | 广西大学 | Molecular breeding method of black-feather green-foot powder-shell meat-egg dual-purpose chicken |
| CN111418548A (en) * | 2020-04-24 | 2020-07-17 | 广东天农食品有限公司 | Breeding method of dwarf yellow chicken strain with homozygous mutation caused by pathogen |
| CN114283882A (en) * | 2021-12-31 | 2022-04-05 | 华智生物技术有限公司 | Nondestructive poultry egg quality character prediction method and system |
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| CN114283882A (en) * | 2021-12-31 | 2022-04-05 | 华智生物技术有限公司 | Nondestructive poultry egg quality character prediction method and system |
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