CN109342747A - A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing - Google Patents
A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing Download PDFInfo
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Abstract
The invention discloses a kind of microtrabeculaes of ABO and RhD blood typing to be aggregated detection card, belongs to medicine technology field.The detection card includes card body, card body is the hollow structure of open top, partition is equipped in card body, the inside of card body is divided into left and right Liang Ge sensing chamber by the partition, two detection interiors are respectively equipped with three and five micro-column gel pipes, the top of card body is equipped with the sealing film for covering all micro-column gel pipe nozzles, and sealing film disconnects at partition, the left and right ends that sealing film is located at card body are respectively equipped with easy tear tape, the position that the obverse and reverse of card body corresponds to Liang Ge sensing chamber is equipped with sign pasting, the middle position of one of sign pasting, which is equipped with, writes item, the fixed plate for fixing card body is rotatably coupled below card body.The configuration of the present invention is simple, it is possible to reduce human error improves result accuracy, and specificity is high, especially has hypersensitivity to the detection of poor antigen and hypotype, reproducible.
Description
Technical field
The present invention relates to a kind of microtrabeculaes of ABO and RhD blood typing to be aggregated detection card, belongs to medicine technology field.
Background technique
The accurately sizing of abo blood group is extremely important, is basis and the guarantee of safe transfusion.Rh blood group system is to be only second to
A most complicated system in the important blood group system and erythrocyte blood type system of ABO blood group system, in Rh blood group system
Antigen include D, C, c, E, e etc., wherein D antigen is most strong, needs to carry out conventional detection.It is anti-that erythrocyte surface contains D
Originally it was the RhD positive, and was RhD negative without containing D antigen, after RhD negative patient receives RhD positive blood, at least 20% patient
Immune response can be generated to D antigen, hemolytic reaction can occur after contacting the antigen in vivo for the second time, therefore determine RhD
Type is also particularly significant.
It is the immune detection new method gradually risen in recent decades that microtrabeculae, which is aggregated detection card, is antibody and accordingly resists
Former red blood cell reaction generates agglutination, cannot can reach bottom by the bottom of microtrabeculae, single red blood cell.Microtrabeculae agglutination medium
Experimental technique is sieve technology and the product that immunological technique combines, and its essence is a kind of red cell antigens and corresponding antibodies to exist
The agglutinating reaction of gel media is utilized in microtrabeculae pipe.Whether the technology can be by special based on free red blood cell and agglutination cell
The gel media of structure, so that the red blood cell of different conditions be enable to separate the progress of this principle.The glucan of specific proportion is solidifying
Glue is sub-packed in special microtrabeculae, and upper layer is " reaction tank " (red cell antigens and corresponding antibodies combined area), and lower layer is " separation
Pond ".Under certain centrifugal force effect, the free red blood cell not in conjunction with antibody can be sunk due to small in size by gel layer
It forms sediment in bottom, is formed " cell button ", be negative reaction；In conjunction with specific antibody or the red blood cell of cohesion due to volume is big by
Cohesion retardance cannot by coacervate, stay in cohesion medium top or be suspended in gel, be positive reaction.
Compare with traditional test tube method or slide method (slide method can only do positive definite form, not can be carried out reverse type) test, it is micro-
The cumbersome washing procedure of traditional test tube liquid medium is omitted in column agglutination detection card, has that sample dosage is few, safe operation is simple
Just, time saving, human factor is small, be easy to standardize and automate, result is reproducible, easy interpretation, it is clear, clear, can protect for a long time
The advantages that depositing, is for future reference, the detection suitable for high-volume sample.
It is widely used in bracket for blood grouping, irregular antibody detection at abroad, microtrabeculae agglutination detection card has become routine and hands over
Pitch the various blood types serological tests such as match.In recent years, it is domestic started to carry out using import microtrabeculae agglutination detection card ABO and
RhD blood typing, but since its price is high, equipment is expensive, it seriously affects it and popularizes at home, in addition, there is also following defects:
First is that price is high, equipment is expensive, seriously affects it and popularize at home；Second is that when operator is newly to enter when carrying out reverse type experiment
When time worker, it is possible to the concentration (red blood cell needs to be made into 1% concentration) of inaccurate antibody screening red blood cell is held, so as to cause
As a result it is not allowed, increases experimental error.Third is that when especially going to work alone, having A, B, O is red when operator is extremely tired
The risk of cell addition mistake, to increase experimental error.
In consideration of it, it is necessary to provide a kind of microtrabeculae agglutination detection card of novel ABO and RhD blood typing, it is existing to solve
There is the deficiency of technology.
Summary of the invention
An object of the present invention is to provide a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing.Of the invention
The microtrabeculae agglutination detection card of ABO and RhD blood typing, structure are simple, it is possible to reduce human error improves result accuracy, special
Anisotropic high, high sensitivity especially has hypersensitivity to the detection of poor antigen, poor antigen D and hypotype, reproducible, operation
It is safe and simple quick.
The technical scheme to solve the above technical problems is that a kind of microtrabeculae of ABO and RhD blood typing is aggregated inspection
Card, including card body are surveyed, the card body is the hollow structure of open top, is equipped with partition, the inside of the card body in the card body
It is divided into left and right Liang Ge sensing chamber by the partition, described two detection interiors are respectively equipped with three micro-column gel pipes and five micro-
Column gel tube, the top of the card body is equipped with the sealing film for covering all micro-column gel pipe nozzles, and the sealing film exists
It is disconnected at the partition, the left and right ends that the sealing film is located at the card body are respectively equipped with easy tear tape, the front of the card body
The position that described two sensing chamber are corresponded to reverse side is equipped with sign pasting, and the middle position of one of them sign pasting, which is equipped with, writes
Item is rotatably coupled the fixed plate for fixing the card body below the card body.
Based on the above technical solution, the present invention can also be improved as follows.
Further, the material of the card body is polyurethane.
Further, described two sensing chamber are respectively the first sensing chamber and the second sensing chamber, wherein first sensing chamber
Three be inside equipped with the micro-column gel pipe is respectively to be used to detect the micro-column gel pipe of anti-A antibody, for detecting anti-B antibody
Micro-column gel pipe and micro-column gel pipe for detecting irregular antibody；Indoor five microtrabeculaes being equipped with of second detection
Gel tube, respectively for detecting the micro-column gel pipe of Staphylococal Protein A, the micro-column gel pipe for detecting B antigen, anti-for detecting D
Former micro-column gel pipe, negative control micro-column gel pipe and own control micro-column gel pipe.
It is using above-mentioned further beneficial effect: the indoor micro-column gel being equipped with for detecting anti-A antibody of the first detection
Pipe, the micro-column gel pipe for detecting anti-B antibody and the micro-column gel pipe for detecting irregular antibody, experimental implementation person are not necessarily to
Standard A type red blood cell, Type B red blood cell, O-shaped red blood cell are added into above three micro-column gel pipe, only need to directly add patient
Serum or blood plasma into above three micro-column gel pipe, after centrifugation, so that it may direct observation experiment result.
Second detection is indoor to be equipped with for detecting Staphylococal Protein A micro-column gel pipe, the micro-column gel pipe for detecting B antigen, uses
In micro-column gel pipe, negative control micro-column gel pipe and the own control micro-column gel pipe of detection D antigen.Work as negative control
It indicates that experiment is qualified for feminine gender, otherwise needs to test again.
Further, the loading amount of each micro-column gel pipe is 150 μ l-200 μ l.
Be using above-mentioned further beneficial effect: certain particular patients 's, as hepatopathy, leukaemia, hemodilution patient,
Because amount of antibody lowers, needs to increase the detection limit of blood plasma, can just measure weak antibody.Above-mentioned loading amount both can satisfy 2 person-portions
Detection needs, and the sample being also possible to prevent in microtrabeculae pipe sputters to come in centrifugation.
Further, it in the micro-column gel pipe for detecting anti-A antibody, is protected containing standard A type red blood cell, red blood cell
Nutrient solution, the glucan being suspended in buffer, the preservative being suspended in buffer and the gel media being suspended in buffer.
Further, it in the micro-column gel pipe for detecting anti-B antibody, is protected containing standard Type B red blood cell, red blood cell
Nutrient solution, the glucan being suspended in buffer, the preservative being suspended in buffer and the gel media being suspended in buffer.
Further, described for detecting in the micro-column gel pipe of irregular antibody, containing the O-shaped red blood cell of standard, it is red carefully
Born of the same parents' Precerving liquid, the glucan being suspended in buffer, the preservative being suspended in buffer and the gel being suspended in buffer
Medium.
Further, it is described for detect in the micro-column gel pipe of Staphylococal Protein A containing reagent monoclonal antibody A, be suspended in it is slow
Glucan, the preservative being suspended in buffer in fliud flushing and the gel media being suspended in buffer.
Further, it is described for detect in the micro-column gel pipe of B antigen containing reagent monoclonal antibody B, be suspended in it is slow
Glucan, the preservative being suspended in buffer in fliud flushing and the gel media being suspended in buffer.
Further, it is described for detect in the micro-column gel pipe of D antigen containing reagent monoclonal antibody D, be suspended in it is slow
Glucan, the preservative being suspended in buffer in fliud flushing and the gel media being suspended in buffer.
Further, in the negative control micro-column gel pipe and the own control micro-column gel pipe, containing suspension
Glucan, the preservative being suspended in buffer in buffer and the gel media being suspended in buffer.
Further, the sealing film is made of primer layer, aluminium foil layer and plastic film layers；The primer layer and the plastics
Film layer is respectively coated in the two sides of the aluminium foil layer, and the primer layer covers the nozzle of the micro-column gel pipe.
Be using above-mentioned further beneficial effect: when experiment, tear initiation seals film, after having added sample blood to be checked,
Sealing film is affixed again.Equipped with sealing film, it can both prevent the reagent in microtrabeculae pipe from volatilizing and generate aerosol, and be also possible to prevent microtrabeculae
Sample in pipe sputters to come in centrifugation.
Further, the rough surface for writing item.
Be using above-mentioned further beneficial effect: writing words are more convenient, to record name and the detection of patient
Time prevents from getting patient information wrong.
Further, end positions further respectively have hanging hole above the card body.
It is using above-mentioned further beneficial effect: is equipped with hanging hole above card body, card can be will test and be suspended on steel
On guide frame, area occupied is reduced, and can prevent detection card is unexpected to topple over.Operator can be placed on steel wire rack with flexible choice,
It is also placed on experimental bench face.
Further, the diameter of the hanging hole is 1mm-2mm.
Further, the lower section of the card body be equipped with horizontally disposed shaft, the card body by the shaft with it is described solid
Fixed board rotatably connects, the fixed plate include be respectively provided at the shaft two sides side plate and be located at two side plates it
Between bottom plate.
It is using above-mentioned further beneficial effect: is equipped with fixed plate below card body, it can be fixed on experiment table top
Card body can prevent detection card is unexpected to topple over without being placed in operator's hand, more convenient.Fixed plate is set as two side plates
With a bottom plate, the structure of a triangle can be formed in this way, it is more stable, conducive to the placement of card body.
One, the technical parameter of the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention:
In 2-25 DEG C of room temperature, be protected from light under the conditions of save, validity period be 1 year, use before the deadline.ABO of the invention and
The microtrabeculae agglutination detection card of RhD blood typing has following technical standard for abo blood group and Rh (D) detection:
1, appearance
Gel in each micro-column gel pipe has 1mm-5mm clarification in uniform pale blue green or milky, glue surface upper end
Transparency liquid, bubble-free or other foreign matters between gel (wherein the anti-A of positive definite form is blue, and anti-B is yellow).
2, sensitivity
Remolding sensitivity test tube method, slide method have ten times or more of raising.
3, specific
It is specific high, anti-potency >=128 A, anti-potency >=128 B, anti-potency >=64 D.
Two, application method of the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention for the detection of blood group:
Step 1: sample to be detected is configured to 0.5%-0.8% red blood cell physiological saline suspension
Reverse type: taking 50 μ l of blood plasma or serum to be checked, is added separately to the micro-column gel pipe for detecting anti-A antibody, uses
In the micro-column gel pipe of detection anti-B antibody and the micro-column gel pipe for detecting irregular antibody.
Positive definite form: prepared 50 μ l of red blood cell physiological saline suspension is taken, the microtrabeculae for detecting Staphylococal Protein A is added separately to
Gel tube, the micro-column gel pipe for examining B antigen, the micro-column gel pipe for detecting D antigen, negative control micro-column gel pipe,
And in own control micro-column gel pipe, also need to add the 50 μ l of blood plasma or serum of patient to be checked in own control gel tube.
Step 2: being centrifuged 2min prior to 900rpm, be centrifuged 3min then at 1500rpm, take out naked eyes and determine result.As a result sentence
Disconnected standard, it is specific as shown in Figure 5.
Principle explanation:
Positive findings: the specific antigen-antibody complex that red cell antigens and corresponding antibodies are formed in micro-column gel is floating
It is positive reaction in gel surface or glue.It is specific as shown in table 1.
Table 1ABO blood group RhD positive identification table
Negative findings: red cell antigens are combined without corresponding antibody, specific antigen-antibody complex, red blood cell do not occur
It is sunken to the sharp bottom of micro-column gel, is negative reaction.It is specific as shown in table 2.
Table 2ABO blood group RhD negative identification table
Response intensity: specific erythrocyte antigen antibody complex is located at glue surface, is strong positive reaction；Compound is in glue
In, it is reacted for weakly positive；More smaller close to glue bottom particles, it reacts weaker.It is specific as shown in table 3.
3 response intensity table of table
| Response intensity | Phenomenon and explanation |
| 4+ | Red blood cell compound (agglutination) is located at gel surface |
| 3+ | Most of red blood cell compound is located at gel surface, and small part is located in the middle part of gel |
| 2+ | Most cells compound is located in the middle part of gel, and small part is located at gel middle and upper part |
| 1+ | Red blood cell compound is located at gel middle and lower part |
| ± | It is compareed with negative tube result in same card, such as there is difference with negative findings, as ± |
| - | Red blood cell is completely deposited at gel tip bottom |
| Complete hemolysis | Without agglutination or unagglomerated red blood cell, there is transparent limpid red in liquid for gel and liquid |
| Incomplete haemolysis | Residual red blood cells are in glue surface, glue or transparent limpid red occur in glue bottom, liquid |
| Mixed agglutination | Red blood cell compound is located at gel surface and gel bottom |
Beneficial effects of the present invention:
(1) the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention, structure are simple, it is possible to reduce artificial to miss
Difference improves result accuracy, and specificity is high, and high sensitivity especially has hypersensitivity to the detection of poor antigen and hypotype, weight
Renaturation is good, simple and efficient to handle.
(2) experiment detection process in, experimental implementation person generally first adds red blood cell, then, then plus patient serum or
Blood plasma.The microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention, distinguishes in advance in 3 micro-column gel pipes wherein
Standard A type red blood cell, Type B red blood cell, O-shaped red blood cell and rbc preservative have been got well in addition, are beneficial to extend antigen-antibody
Reaction reduced because of the case where antigen-antibody reaction is insufficient and the weak type antibody of the certain hypotype blood groups of missing inspection, improved correct
Recall rate.
Detailed description of the invention
Fig. 1 is the rearview of the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention.
Fig. 2 is the front view of the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention.
Fig. 3 is the partial enlarged view of component 5 in Fig. 1.
Wherein, component representated by each label is as follows:
1, card body, 2, partition, 3, sensing chamber, the 31, first sensing chamber, the 32, second sensing chamber, 4, micro-column gel pipe, 41, use
In the micro-column gel pipe of detection anti-A antibody, 42, the micro-column gel pipe for detecting anti-B antibody, 43, irregular anti-for detecting
The micro-column gel pipe of body, 44, the micro-column gel pipe for detecting Staphylococal Protein A, 45, the micro-column gel pipe for detecting B antigen, 46,
For detecting the micro-column gel pipe of D antigen, 47, negative control micro-column gel pipe, 48, control micro-column gel pipe, 5, sealing film,
51, primer layer, 52, aluminium foil layer, 53, plastic film layers, 6, easy tear tape, 7, sign pasting, 8, writing item, 9, hanging hole, 10, fixation
Plate, 10-1, side plate, 10-2, bottom plate, 11, shaft.
Fig. 4 is the partial enlarged view in Fig. 1 at A.
Fig. 5 is the result judgement figure using the microtrabeculae agglutination detection card of ABO and RhD blood typing of the invention.Wherein, from
The micro-column gel pipe of left-to-right, respectively represent experimental result be 4+, 3+, 2+, 1+, ± ,-, complete hemolysis, incomplete haemolysis and mixed
Close agglutination.
Specific embodiment
Principles and features of the present invention are described below in conjunction with specific attached drawing, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
As Figure 1-Figure 4, the microtrabeculae agglutination detection card of the ABO and RhD blood typing of the present embodiment, including card body 1, institute
The hollow structure that card body 1 is open top is stated, partition 2 is equipped in the card body 1, the inside of the card body 1 passes through the partition 2
It is divided into left and right Liang Ge sensing chamber 3, three micro-column gel pipes 4 and five micro-column gel pipes is respectively equipped in described two sensing chamber 3
4, the top of the card body 1 is equipped with the sealing film 5 for covering all 4 nozzles of micro-column gel pipe, and the sealing film 5 is described
It is disconnected at partition 2, the left and right ends that the sealing film 5 is located at the card body 1 are respectively equipped with easy tear tape 6, the front of the card body 1
The position that described two sensing chamber 3 are corresponded to reverse side is equipped with sign pasting 7, and the middle position of one of them sign pasting 7 is equipped with
Item 8 is write, the lower section of the card body 1 is rotatably coupled the fixed plate 10 for fixing the card body 1.
The material of the card body 1 is polyurethane.
Described two sensing chamber 3 are respectively the first sensing chamber 31 and the second sensing chamber 32, wherein first sensing chamber 31
Three be inside equipped with the micro-column gel pipe 4 is respectively for detecting the micro-column gel pipe 41 of anti-A antibody, resisting for detecting anti-B
The micro-column gel pipe 42 of body and micro-column gel pipe 43 for detecting irregular antibody；Five be equipped in second sensing chamber 32
A micro-column gel pipe 4, respectively micro-column gel pipe 44, the micro-column gel pipe for detecting B antigen for detecting Staphylococal Protein A
45, for detecting the micro-column gel pipe 46, negative control micro-column gel pipe 47 and own control micro-column gel pipe 48 of D antigen.
First detection is indoor to be equipped with the micro-column gel pipe for detecting anti-A antibody, the micro-column gel for detecting anti-B antibody
Pipe and the micro-column gel pipe for detecting irregular antibody, experimental implementation person without add standard A type red blood cell, Type B red blood cell,
For O-shaped red blood cell into above three micro-column gel pipe, serum or the blood plasma that only need to directly add patient are solidifying to above three microtrabeculae
In sebific duct, after centrifugation, so that it may direct observation experiment result.
Second detection is indoor to be equipped with for detecting Staphylococal Protein A micro-column gel pipe, the micro-column gel pipe for detecting B antigen, uses
In micro-column gel pipe, negative control micro-column gel pipe and the own control micro-column gel pipe of detection D antigen.Work as negative control
It indicates that experiment is qualified for feminine gender, otherwise needs to test again.
The loading amount of each micro-column gel pipe 4 is 150 μ l-200 μ l.Certain particular patients 's, as hepatopathy, leukaemia,
The patient of hemodilution needs to increase the detection limit of blood plasma, can just measure weak antibody because amount of antibody lowers.Above-mentioned loading amount, both
It can satisfy the detection needs of 2 person-portions, the sample being also possible to prevent in microtrabeculae pipe sputters to come in centrifugation.
In the micro-column gel pipe 41 for detecting anti-A antibody, containing standard A type red blood cell, rbc preservative, mix
The glucan being suspended from buffer, the preservative being suspended in buffer and the medium being suspended in buffer.
In the micro-column gel pipe 42 for detecting anti-B antibody, containing standard Type B red blood cell, rbc preservative, mix
The glucan being suspended from buffer, the preservative being suspended in buffer and the medium being suspended in buffer.
In the micro-column gel pipe 43 for detecting irregular antibody, maintained containing the O-shaped red blood cell of standard, red blood cell
Liquid, the glucan being suspended in buffer, the preservative being suspended in buffer and the medium being suspended in buffer.
It is described for detecting in the micro-column gel pipe 44 of Staphylococal Protein A containing reagent monoclonal antibody A, being suspended in buffer
Glucan, the preservative being suspended in buffer and the gel media being suspended in buffer.
It is described for detecting in the micro-column gel pipe 45 of B antigen containing reagent monoclonal antibody B, being suspended in buffer
Glucan, the preservative being suspended in buffer and the gel media being suspended in buffer.
It is described for detecting in the micro-column gel pipe 46 of D antigen containing reagent monoclonal antibody D, being suspended in buffer
Glucan, the preservative being suspended in buffer and the gel media being suspended in buffer.
In the negative control micro-column gel pipe 47 and the own control micro-column gel pipe 48, contains and be suspended in buffering
Glucan, the preservative being suspended in buffer in liquid and the gel media being suspended in buffer.
The sealing film 5 is made of primer layer 51, aluminium foil layer 52 and plastic film layers 53；The primer layer 51 and the modeling
Material film layer 53 is respectively coated in the two sides of the aluminium foil layer 52, and the primer layer 51 covers the micro-column gel pipe 4
Nozzle.When experiment, tear initiation seals film, after having added sample blood to be checked, then affixes sealing film.It, both can be with equipped with sealing film
It prevents the reagent in microtrabeculae pipe from volatilizing and generates aerosol, the sample being also possible to prevent in microtrabeculae pipe sputters to come in centrifugation.
The rough surface for writing item 8.Writing words are more convenient, to record the name and detection time of patient,
It prevents from getting patient information wrong.
The 1 top end positions of card body further respectively have hanging hole 9.It is equipped with hanging hole above card body, can will examine
It surveys card to be suspended on steel wire rack, reduces area occupied, and can prevent detection card is unexpected to topple over.Operator can be to put with flexible choice
On steel wire rack, also it is placed on experimental bench face.
The diameter of the hanging hole 9 is 1mm-2mm.
The lower section of the card body 1 is equipped with horizontally disposed shaft 11, and the card body 1 passes through the shaft 11 and the fixation
Plate 10 rotatably connects, and the fixed plate includes being respectively provided at the side plate 10-1 of 1 two sides of shaft and being located at described in two
Bottom plate 10-2 between side plate 10-1.It is equipped with fixed plate below card body, card body can be fixed on experiment table top, without being placed on
In operator's hand, it can prevent detection card is unexpected to topple over, it is more convenient.Fixed plate is set as two side plates and a bottom plate, this
Sample can form the structure of a triangle, more stable, conducive to the placement of card body.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing, which is characterized in that including card body (1), the card body (1)
For the hollow structure of open top, the card body (1) is interior to be equipped with partition (2), and the inside of the card body (1) passes through the partition
(2) it is divided into left and right Liang Ge sensing chamber (3), is respectively equipped with three micro-column gel pipes (4) in described two sensing chamber (3) and five micro-
Column gel tube (4), the top of the card body (1) are equipped with the sealing film (5) for covering all micro-column gel pipe (4) nozzles, and
The sealing film (5) disconnects at the partition (2), and the left and right ends that sealing film (5) is located at the card body (1) are set respectively
Having easy tear tape (6), the position that the obverse and reverse of the card body (1) corresponds to described two sensing chamber (3) is equipped with sign pasting (7),
The middle position of one of them sign pasting (7), which is equipped with, writes item (8), has been rotatably coupled below the card body (1)
For fixing the fixed plate (10) of the card body (1).
2. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 1, which is characterized in that described
The material of card body (1) is polyurethane.
3. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 1, which is characterized in that described
Liang Ge sensing chamber (3) is respectively the first sensing chamber (31) and the second sensing chamber (32), wherein is set in first sensing chamber (31)
Three had the micro-column gel pipe (4) is respectively for detecting the micro-column gel pipe (41) of anti-A antibody, resisting for detecting anti-B
The micro-column gel pipe (42) of body and micro-column gel pipe (43) for detecting irregular antibody；It is set in second sensing chamber (32)
Five had the micro-column gel pipe (4), respectively for detecting the micro-column gel pipe (44) of Staphylococal Protein A, for detecting B antigen
Micro-column gel pipe (45), the micro-column gel pipe (46) for detecting D antigen, negative control micro-column gel pipe (47) and itself
It compares micro-column gel pipe (48).
4. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 3, which is characterized in that each
The loading amount of the micro-column gel pipe (4) is 150 μ l-200 μ l.
5. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 3, which is characterized in that described
In micro-column gel pipe (41) for detecting anti-A antibody, containing standard A type red blood cell, rbc preservative, it is suspended in buffer
In glucan, the preservative being suspended in buffer and the gel media being suspended in buffer, standard A type red blood cell use
Fresh blood preparation, antigenicity are stablized, completely.In the micro-column gel pipe (42) for detecting anti-B antibody, contain standard Type B
Red blood cell, rbc preservative, the glucan being suspended in buffer, the preservative being suspended in buffer and it is suspended in buffering
Gel media in liquid, standard Type B red blood cell are prepared using fresh blood, and antigenicity is stablized, completely.It is described irregular for detecting
In the micro-column gel pipe (43) of antibody, containing the O-shaped red blood cell of standard, rbc preservative, the glucan being suspended in buffer,
The preservative being suspended in buffer and the gel media being suspended in buffer, standard type O red blood cell are prepared using fresh blood,
Antigenicity is stablized, completely.
6. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 3, which is characterized in that described
Contain the reagent monoclonal antibody A, glucan being suspended in buffer, mixed in micro-column gel pipe (44) for detecting Staphylococal Protein A
The preservative being suspended from buffer and the gel media being suspended in buffer；The micro-column gel pipe for being used to detect B antigen
(45) contain reagent monoclonal antibody B, the glucan being suspended in buffer, the preservative being suspended in buffer and suspension in
Gel media in buffer；It is described for detect in the micro-column gel pipe (46) of D antigen containing reagent monoclonal antibody D,
The glucan being suspended in buffer, the preservative being suspended in buffer and the gel media being suspended in buffer；It is described
In negative control micro-column gel pipe (47) and the own control micro-column gel pipe (48), containing the Portugal being suspended in buffer
Glycan, the preservative being suspended in buffer and the gel media being suspended in buffer.
7. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 3, which is characterized in that described
Sealing film (5) is made of primer layer (51), aluminium foil layer (52) and plastic film layers (53)；The primer layer (51) and the plastics
Film layer (53) is respectively coated in the two sides of the aluminium foil layer (52), and the primer layer (51) covers the micro-column gel pipe
(4) nozzle.
8. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 1, which is characterized in that described
Write the rough surface of item (8).
9. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 1, which is characterized in that described
End positions further respectively have hanging hole (9) above card body (1).
10. a kind of microtrabeculae agglutination detection card of ABO and RhD blood typing according to claim 1, which is characterized in that institute
The lower section for stating card body (1) is equipped with horizontally disposed shaft (11), and the card body (1) passes through the shaft (11) and the fixed plate
(10) rotatable connection, the fixed plate include being respectively provided at the side plate (10-1) of the shaft (1) two sides and being located at two
Bottom plate (10-2) between the side plate (10-1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811209535.4A CN109342747A (en) | 2018-10-17 | 2018-10-17 | A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811209535.4A CN109342747A (en) | 2018-10-17 | 2018-10-17 | A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109342747A true CN109342747A (en) | 2019-02-15 |
Family
ID=65309200
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811209535.4A Pending CN109342747A (en) | 2018-10-17 | 2018-10-17 | A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing |
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| CN (1) | CN109342747A (en) |
Citations (7)
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|---|---|---|---|---|
| CN101701961A (en) * | 2009-11-25 | 2010-05-05 | 江阴力博医药生物技术有限公司 | Method for preparing typing detection reagent card of blood types of A, B and O |
| CN101718794A (en) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Preparation method of ABO/RhD blood typing detection reagent card |
| CN102435756A (en) * | 2011-09-21 | 2012-05-02 | 长春博迅生物技术有限责任公司 | Preparation method of detection card for detecting ABO and RhD blood groups |
| CN204925135U (en) * | 2015-07-07 | 2015-12-30 | 江苏中济万泰生物医药有限公司 | Rh blood group antigen detects card |
| CN205620420U (en) * | 2016-03-08 | 2016-10-05 | 上海润普生物技术有限公司 | Rh blood group antigen detection card |
| CN206531864U (en) * | 2017-03-15 | 2017-09-29 | 上海润普生物技术有限公司 | A kind of Rh blood type test cards |
| CN209198474U (en) * | 2018-10-17 | 2019-08-02 | 深圳市龙华区中心医院 | A kind of microtrabeculae agglutination detection card of ABO and RhD blood typing |
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2018
- 2018-10-17 CN CN201811209535.4A patent/CN109342747A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101701961A (en) * | 2009-11-25 | 2010-05-05 | 江阴力博医药生物技术有限公司 | Method for preparing typing detection reagent card of blood types of A, B and O |
| CN101718794A (en) * | 2009-11-25 | 2010-06-02 | 江阴力博医药生物技术有限公司 | Preparation method of ABO/RhD blood typing detection reagent card |
| CN102435756A (en) * | 2011-09-21 | 2012-05-02 | 长春博迅生物技术有限责任公司 | Preparation method of detection card for detecting ABO and RhD blood groups |
| CN204925135U (en) * | 2015-07-07 | 2015-12-30 | 江苏中济万泰生物医药有限公司 | Rh blood group antigen detects card |
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