CN109337935A - A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency - Google Patents
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency Download PDFInfo
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- CN109337935A CN109337935A CN201811594530.8A CN201811594530A CN109337935A CN 109337935 A CN109337935 A CN 109337935A CN 201811594530 A CN201811594530 A CN 201811594530A CN 109337935 A CN109337935 A CN 109337935A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention discloses a kind of microwave method for preprocessing for improving vinegar grain anaerobic fermentation efficiency, comprising the following steps: S1, mixes vinegar grain with water, stirs evenly to be placed under microwave condition and pre-processed；S2, proper amount of fresh vinegar grain will be added in anaerobic activated sludge, tamed；S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculum concentration be 60~80%, be placed in anaerobic ferment devices and ferment.Method provided by the invention in a short time destroys the structure of vinegar grain, dissolves out more dissolved organic matters, while accelerating the hydrolytic process of vinegar grain anaerobic fermentation process, improves the efficiency of vinegar grain anaerobic digestion methane phase.
Description
Technical field
The invention belongs to solid waste technical field of biotransformation, in particular to a kind of raising vinegar grain anaerobic fermentation efficiency
Microwave method for preprocessing.
Background technique
China's poor resource of vinegar is abundant, and the vinegar grain that annual production is discharged has acid big, difficult decomposition etc. up to 3,000,000 tons or so
Feature.Currently, most of vinegar grain is still handled in a manner of landfill, but a large amount of methane isothermal chamber gases and high concentration sour water can be generated
Percolate destroys soil, pollutes environment；Fraction is used to make animal feed and culture medium of edible fungus, but its drying cost is high,
Culture medium of edible fungus consumption is few, therefore cannot be from substantially solving the problems, such as.The organic matter content of vinegar grain is abundant, is carried out
Anaerobic fermentation produces renewable energy methane gas, while the biogas residue and biogas liquid for the generation that can also ferment can do ecological organic fertilier.
Vinegar grain contains a large amount of organic principle, predominantly cellulose, hemicellulose, lignin and protein.Due to its spy
Different design feature, by vinegar grain, directly as raw material anaerobic fermentation, there are still certain technical problems, if biological transformation ratio is low, produce
Gas velocity rate is low and the production gas period is long etc..In general, the hydrolytic process of lignocellulose raw material anaerobic fermentation is anaerobic fermentation
Rate-limiting step, therefore the effective pretreatment of progress can promote its hydrolytic process, improve raw material methane production rate.
Summary of the invention
Goal of the invention, bright for vinegar grain high microsteping content and acid big feature, the present invention provides a kind of raising vinegar grain and detests
The microwave method for preprocessing of aerobe fermentation efficiency accelerates vinegar grain hydrolysis rate, and improves methane phase efficiency.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, vinegar grain is mixed with water, stirs evenly to be placed under microwave condition and is pre-processed；
S2, the new fresh vinegar grain of 40~60g is added in 70L anaerobic activated sludge, tamed；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 60~80%, and places it in and carry out anaerobic fermentation in anaerobic ferment devices.
Further, in the step S1, the mass ratio of vinegar grain and water is 1:1.
Further, in the step S1, microwave condition is 180~380W, handles 5~15min of time.
Further, in the step S2, domestication is placed under the conditions of 33~37 DEG C, and initial TS control is 3~5%, initial pH
Control is 7.3~7.8.
Further, in the step S2, when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then
Carry out step S3.
Further, in the step S3, when anaerobic fermentation, anaerobic ferment devices are placed in progress anaerobism hair in water-bath
Ferment.
Further, the water-bath kettle temperature control is at 33~37 DEG C.
Further, in the step S3, the time of anaerobic fermentation is 45d.
Compared with prior art, the invention has the following advantages:
Method provided by the invention in a short time destroys the structure of vinegar grain, dissolves out more dissolved organic matters,
The hydrolytic process for accelerating vinegar grain anaerobic fermentation process simultaneously, improves the efficiency of vinegar grain anaerobic digestion methane phase.
Detailed description of the invention
Fig. 1 is resolvable chemical oxygen demand and Volatile fatty acid contents schematic diagram after different condition pretreatment；
Fig. 2 is accumulation methane production schematic diagram after different condition pretreatment.
Specific embodiment
Below with reference to embodiment, the present invention will be further explained.
Embodiment 1(30% power 5min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of vinegar factory of Jiangsu Province is taken to carry out following anaerobic fermentation, the pH of the vinegar grain is 3.8, solid content 33%, slightly
Fiber content is 27%.
S2, after taking 80g vinegar grain to mix with 80g water, in 180W(30% power) microwave condition under handle 5min.
S3, it takes a small amount of new fresh vinegar grain to be added into anaerobic activated sludge, is tamed under the conditions of 35 DEG C, initial TS control
4%, initial pH control is 7.5, when system methane content is tamed successfully up to 65% or more.
S4, by the vinegar grain mixed liquor that is inoculated in step S2 that treated of the anaerobic activated sludge after being tamed in step S3,
Inoculum concentration is 70%, and mixture is placed in 1.2L fermentor, is placed in 35 DEG C of water-bath, anaerobic fermentation 45d.
S5, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 2(30% power 10min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, after mixing evenly, is placed in 180W(30% power) it pre-processes under microwave condition
10min；
S2, the new fresh vinegar grain of 40g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 33 DEG C, initial TS control is
3%, initial pH control is 7.3, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 60%, and is placed it in fermentor, is put into water-bath, and temperature is controlled in 33 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 3(30% power 15min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of vinegar factory of Jiangsu Province is taken to carry out following anaerobic fermentation, the pH of the vinegar grain is 3.5, solid content 31%, slightly
Fiber content is 26.38%.
S2, after taking 80g vinegar grain to mix with 80g water, in 180W(30% power) microwave condition under handle 15min.
S3, it takes a small amount of new fresh vinegar grain to be added into anaerobic activated sludge, is tamed under the conditions of 35 DEG C, initial TS control
4%, initial pH control is 7.5, when system methane content is tamed successfully up to 65% or more.
S4, by the vinegar grain mixed liquor that is inoculated in step S2 that treated of the anaerobic activated sludge after being tamed in step S3,
Inoculum concentration is 70%, and mixture is placed in 1.2L fermentor, is placed in 35 DEG C of water-bath, anaerobic fermentation 45d.
S5, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 4(45% power 5min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, after mixing evenly, is placed in 330W(45% power) it pre-processes under microwave condition
5min；
S2, the new fresh vinegar grain of 50g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 35 DEG C, initial TS control is
4%, initial pH control is 7.4, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 70%, and is placed it in fermentor, is put into water-bath, and temperature is controlled in 35 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 5(45% power 10min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, after mixing evenly, is placed in 330W(45% power) it pre-processes under microwave condition
10min；
S2, the new fresh vinegar grain of 60g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 37 DEG C, initial TS control is
5%, initial pH control is 7.8, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 80%, and is placed it in fermentor, is put into water-bath, and temperature is controlled in 37 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 6(45% power 15min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, after mixing evenly, is placed in 330W(45% power) it pre-processes under microwave condition
15min；
S2, the new fresh vinegar grain of 40g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 33 DEG C, initial TS control is
4%, initial pH control is 7.5, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 70%, and is placed it in fermentor, is put into water-bath, and temperature is controlled in 35 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 7(60% power 5min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of vinegar factory of Jiangsu Province is taken to carry out following anaerobic fermentation, the pH of the vinegar grain is 4.0, solid content 35%, slightly
Fiber content is 27.022%.
S2, after taking 80g vinegar grain to mix with 80g water, in 380W(60% power) microwave condition under handle 5min respectively.
S3, it takes a small amount of new fresh vinegar grain to be added into anaerobic activated sludge, is tamed under the conditions of 35 DEG C, initial TS control
4%, initial pH control is 7.5, when system methane content is tamed successfully up to 65% or more.
S4, by the vinegar grain mixed liquor that is inoculated in step S2 that treated of the anaerobic activated sludge after being tamed in step S3,
Inoculum concentration is 80%, and mixture is placed in 1.2L fermentor, is placed in 35 DEG C of water-bath, anaerobic fermentation 45d.
S5, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 8(60% power 10min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, after mixing evenly, is placed in 380W(60% power) it pre-processes under microwave condition
10min；
S2, the new fresh vinegar grain of 50g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 37 DEG C, initial TS control is
3%, initial pH control is 7.8, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 80%, and is placed it in fermentor, is put into water-bath, and temperature is controlled in 33~37 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Embodiment 9(60% power 15min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of vinegar factory of Jiangsu Province is taken to carry out following anaerobic fermentation, the pH of the vinegar grain is 3.8, solid content 33%, slightly
Fiber content is 26.5%.
S2, after taking 80g vinegar grain to mix with 80g water, in 380W(60% power) microwave condition under handle 15min.
S3, it takes a small amount of new fresh vinegar grain to be added into anaerobic activated sludge, is tamed under the conditions of 35 DEG C, initial TS control
3%, initial pH control is 7.3, when system methane content is tamed successfully up to 65% or more.
S4, by the vinegar grain mixed liquor that is inoculated in step S2 that treated of the anaerobic activated sludge after being tamed in step S3,
Inoculum concentration is 60%, and mixture is placed in 1.2L fermentor, is placed in 35 DEG C of water-bath, anaerobic fermentation 45d.
S5, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
Comparative example 1(0% power 0min)
A kind of microwave method for preprocessing improving vinegar grain anaerobic fermentation efficiency, comprising the following steps:
S1, the vinegar grain of mass ratio 1:1 is mixed with water, is then stirred evenly；
S2, the new fresh vinegar grain of 50g being added in 70L anaerobic activated sludge, being tamed under the conditions of being placed in 35 DEG C, initial TS control is
4%, initial pH control is 7.5, and when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3；
S3, it step S2 is tamed to successful anaerobic activated sludge is inoculated in the vinegar grain mixture by step S1, inoculum concentration is
70%, and place it in fermentor, it is put into water-bath, temperature is controlled in 35 DEG C of anaerobic fermentation 45d.
S4, take respectively pretreated vinegar grain measure its resolvable chemical oxygen demand and volatile fatty acid (acetic acid, third
Acid) content, while methane production is measured in anaerobic fermentation process.
As shown in Figure 1, resolvable chemical oxygen demand in comparative example 1, embodiment 1, embodiment 3, embodiment 7 and embodiment 9
And Volatile fatty acid contents,
In comparative example 1, COD 92.3mg/L, acetic acid content 1067mg/L；
In embodiment 1, COD 156.4mg/L, acetic acid content 1165mg/L；
In embodiment 3, COD 251.2mg/L, acetic acid content 1228mg/L；
In embodiment 7, COD 196mg/L, acetic acid content 1180mg/L；
In embodiment 9, COD 302.4mg/L, acetic acid content 1412mg/L；
Above-mentioned data explanation, without in the comparative example 1 of microwave treatment, COD and acetic acid content are minimum, embodiment 9
In, processing 60% power of microwave condition (380W) handles 15min, COD and acetic acid content highest, in particular, microwave
60% power of condition (380W) is handled in the vinegar grain mixed liquor of 15min, resolvable chemical oxygen demand and volatile organic acids (acetic acid)
More untreated vinegar grain has been respectively increased 227% and 15%, and wherein the content of propionic acid reduces 1%.Microwave Pretreatment is to raw material
Hydrolytic process play preferable facilitation.
As shown in Fig. 2, in fermentation time passage comparative example 1, embodiment 1, embodiment 3, embodiment 7 and embodiment 9
Methane production variation is accumulated,
In comparative example 1, as fermentation time elapses, methane production is accumulated since 0, is gradually increased, until fermentation reaches on the 45th day
To peak value 2400.38mL/gvs；
In embodiment 1, as fermentation time elapses, methane production is accumulated since 0, is gradually increased, until fermentation reaches on the 45th day
To peak value 2586.21mL/gvs；
In embodiment 3, as fermentation time elapses, methane production is accumulated since 0, is gradually increased, until fermentation reaches on the 45th day
To peak value 2901.65mL/gvs；
In embodiment 7, as fermentation time elapses, methane production is accumulated since 0, is gradually increased, until fermentation reaches on the 45th day
To peak value 3277.45mL/gvs；
In embodiment 9, as fermentation time elapses, methane production is accumulated since 0, is gradually increased, until fermentation reaches on the 45th day
To peak value 6251.85mL/gvs；
Above-mentioned data explanation, without in the comparative example 1 of microwave treatment, accumulation methane production is minimum, in embodiment 9, handles micro-
60% power of wave condition (380W) handles 15min, methane production highest is accumulated, in particular, 60% power of microwave condition (380W)
The total methane production of vinegar grain that the vinegar grain of processing 15min is more untreated improves 160%, and gas production rate improves 160%.Microwave
Pretreatment can be effectively facilitated the methane phase efficiency of vinegar grain anaerobic fermentation, simultaneously effective improve methane phase rate.
The above is only a preferred embodiment of the present invention, it should be pointed out that: for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of microwave method for preprocessing for improving vinegar grain anaerobic fermentation efficiency, which comprises the following steps:
S1, vinegar grain is mixed with water, stirs evenly to be placed under microwave condition and is pre-processed；
S2, the new fresh vinegar grain of 40~60g is added in 70L anaerobic activated sludge, tamed；
S3, step S2 is tamed successful anaerobic activated sludge be inoculated in by step S1 handle vinegar grain mixture in, inoculation
Amount is 60~80%, and places it in and carry out anaerobic fermentation in anaerobic ferment devices.
2. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: institute
It states in step S1, the mass ratio of vinegar grain and water is 1:1.
3. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
In step S1, microwave condition is 180~380W, handles 5~15min of time.
4. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
In step S2, domestication is placed under the conditions of 33~37 DEG C, and initial TS control is 3~5%, and initial pH control is 7.3~7.8.
5. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
In step S2, when system methane content is up to 65% or more, anaerobic activated sludge is tamed successfully, then carries out step S3.
6. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
In step S3, when anaerobic fermentation, anaerobic ferment devices is placed in water-bath and carry out anaerobic fermentation.
7. the microwave method for preprocessing according to claim 6 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
Water-bath kettle temperature is controlled at 33~37 DEG C.
8. the microwave method for preprocessing according to claim 1 for improving vinegar grain anaerobic fermentation efficiency, it is characterised in that: described
In step S3, the time of anaerobic fermentation is 45d.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2794885A1 (en) * | 2010-03-31 | 2011-10-13 | Asahi Kasei Chemicals Corporation | Cellulose composite |
| CN105385559A (en) * | 2015-11-24 | 2016-03-09 | 全椒县荣昌葡萄种植专业合作社 | Fruit vinegar brewed by using waste grape pomace |
| CN106591377A (en) * | 2016-12-07 | 2017-04-26 | 南阳师范学院 | Anaerobic fermentation treatment method for cassava vinasse |
-
2018
- 2018-12-25 CN CN201811594530.8A patent/CN109337935A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2794885A1 (en) * | 2010-03-31 | 2011-10-13 | Asahi Kasei Chemicals Corporation | Cellulose composite |
| CN105385559A (en) * | 2015-11-24 | 2016-03-09 | 全椒县荣昌葡萄种植专业合作社 | Fruit vinegar brewed by using waste grape pomace |
| CN106591377A (en) * | 2016-12-07 | 2017-04-26 | 南阳师范学院 | Anaerobic fermentation treatment method for cassava vinasse |
Non-Patent Citations (5)
| Title |
|---|
| LU FENG 等: ""Biochemical methane potential(BMP) of vinegar residue and the influence of feed to inoculum ratios on biogas production"", 《BIORESOURCES》 * |
| ZHENBIN WANG等: ""Pretreatment of vinegar residue and anaerobic sludge for enhanced hydrogen and methane production in the two-stage anaerobic system"", 《INTERNATIONAL JOURNAL O F HYDROGEN ENERGY》 * |
| 丁利等: ""醋渣和芦苇混合厌氧消化产气潜力测定"", 《中国沼气》 * |
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Application publication date: 20190215 |