CN109266696A - A kind of method that white-rot fungi preconditioning technique improves mushroom residue anaerobic fermentation efficiency - Google Patents
A kind of method that white-rot fungi preconditioning technique improves mushroom residue anaerobic fermentation efficiency Download PDFInfo
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- CN109266696A CN109266696A CN201811173049.1A CN201811173049A CN109266696A CN 109266696 A CN109266696 A CN 109266696A CN 201811173049 A CN201811173049 A CN 201811173049A CN 109266696 A CN109266696 A CN 109266696A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
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Abstract
The present invention relates to organic solid castoff resource technology fields, and in particular to a kind of method that white-rot fungi preconditioning technique improves mushroom residue hydrolysis acidification efficiency.Preparation method is as follows: being pretreatment substrate with high lignin content mushroom residue, mushroom residue after drying is mechanically pulverized, under sterile conditions by 0.1-0.5 mass inoculation Pleurotus Sajor Caju (MES 03464), pre-processed 4-6 weeks under conditions of 25 DEG C, humidity 75%.Anaerobically digested sludge is seed sludge, and water is added to be configured to the mixed liquor of solid content 12%-18%, and mixed liquor is mixed with seed sludge by volatile organic matter amount ratio 1:2, nitrogen 3-5min is filled with；Controlling reaction temperature is 37 DEG C, and mixing speed 150-160r/min carries out fermentation 6-9d under anaerobic environment；The mixed liquor after fermentation is centrifugated using centrifuge, centrifugal speed 15000r/min, the supernatant after centrifugation is VFA fermentation liquid.The technological operation is simple, economic cost is cheap, has a good application prospect.The present invention provides a kind of new method for mushroom residue disposal of resources.
Description
Technical field
The present invention relates to organic solid castoff resource technology fields, and in particular to a kind of to be pre-processed using white-rot fungi
The choice of technology cracks the method that lignin promotes mushroom residue anaerobic fermentation production volatile fatty acid (VFA).
Background technique
Mushroom residue is the by-product in mushroom production process, is mainly made of cellulose, hemicellulose and lignin, carbon nitrogen
Than high, while the elements such as nitrogen rich in, phosphorus, potassium.Currently, measure is effectively treated in most of mushroom residue shortage, by random heap
It puts, results in waste of resources, while being easy to bring the secondary pollution to environment.Although there is small part mushroom residue to be used for soil improvement
Agent or animal feed, but these method disposing capacities are low, added value of product is low, cannot be effectively relieved that mushroom residue largely accumulates asks
Topic, also limits the large-scale production of mushroom to a certain extent.Therefore, it rationally disposes and further increases to the comprehensive of mushroom residue
It closes and utilizes, reduce potential risk of environmental pollution, or even its resource utilization is of great significance.
Anaerobic digestion techniques obtain extensively in the stabilisation, recycling and minimizing of the biomass such as whole world lignocellulosic
Using.Organic substrates pass through hydrolysis acidification, generate a large amount of metal carboxylate, and main component is the short chains such as acetic acid, propionic acid, butyric acid
Fatty acid.These short chain carboxy acid's salt are the important raw materials for production of organic chemical industry's industry, and added value is much larger than methane, are moulded in biology
The synthesis of material, the production of bioenergy and the denitrogenation dephosphorizing of waste water etc. the potentiality that have a wide range of applications.In mushroom residue structure
Lignin the accessible property of cellulose and enzyme is limited to the unformed biodegradation process that is bundled in of cellulose, hinder micro- life
Object further utilizes substrate, it is caused to reduce in anaerobic digestion efficiency.Can effectively it be changed by preprocess method appropriate
Lignocellulosic structure improves the anaerobism biodegradability of lignocellulose-like biomass.White-rot fungi is extracellular wooden by secreting
Plain degrading enzyme, such as lignin-degrading enzymes, manganese peroxidase, laccase, can effectively lignin degrading.In addition, some white
Rotten fungi has the function of Selective lignin-degradation, i.e., only loses a small amount of cellulose, improves substrate cellulose and lignin
Ratio promotes the anaerobic digestion efficiency of lignocellulose-like biomass.
This patent is pre-processed using high lignin content mushroom residue as raw material by white-rot fungi, and effective selectivity cracks wood
Quality carries out anaerobic fermentation under conditions of no pH is controlled and produces VFA, and the organic acid of generation is as the production having compared with high added value
Product can be used as producing the materials such as medium chain fatty acid, provide a new approaches for the disposal of resources of mushroom residue.This method produces work
Skill is simple, with good economic, society and environment benefit.
Summary of the invention
1. goal of the invention
It is an object of the present invention to reduce mushroom residue aiming at the problem that anaerobic fermentation efficiency high lignin content, provide
A kind of lignin using in white-rot fungi preconditioning technique degradation selectivity mushroom residue, improve mushroom residue anaerobism biodegradability and
VFA yield, the minimizing of realization mushroom residue waste, innoxious, recycling.The VFA produced by means of the present invention has wide
Wealthy application prospect.
2. technical solution
The present invention provides a kind of acid production rate of white-rot fungi pretreatment raising high lignin content mushroom residue, is efficiently waved
The process of hair property fatty acid, comprising the following steps:
(1) white-rot fungi culture: white-rot fungi of the present invention using Pleurotus Sajor Caju (P.sajor-caju,
MES 03464) bacterial strain, conservation is in 3% malt-agar culture.Aseptically, the bacterium of 3-5 block diameter 1-2cm is accessed
Cake to be equipped with 1.5L sterilizing sorghum bead culture box.Sufficiently shaking culture box is uniformly distributed mycelia, places about 15 at room temperature
It, until sorghum surface covers with white hypha.
(2) mushroom residue pre-processes: taking a certain amount of mushroom residue, dries after 48h to constant weight, be mechanically pulverized to partial size
1.5-3.0cm being used for subsequent white-rot fungi preprocessing process；
(3) white-rot fungi pre-processes: by pretreated mushroom residue in 121 DEG C of sterilizing 30min, pressing 0.1~0.5 after cooling
Mass ratio adds the sorghum bead with mycelia into preatreating reactors, and inoculation volume accounted for about the 1/3 of entire reactor.It connects
After kind, sufficiently stirring mushroom residue comes into full contact with fungi with it.Sample is transferred to 25 DEG C, is pre-processed under conditions of humidity 75%
4-6 weeks；
(4) seed sludge pre-processes: by anaerobically digested sludge in 105 DEG C of heating 20-30min, removing methane phase therein
Bacterium, the seed sludge as mushroom residue fermentation；
(5) mushroom residue anaerobic fermentation: by seed sludge in pretreated mushroom residue in step (3) and step (4) by waving
Anaerobic fermentation reactor is added in hair property organic matter mass ratio 1:2, adds water to be configured to the mixed liquor of solid content 12%-18%, nitrogen charging
Gas 3-5min, control reaction temperature are 37 DEG C, mixing speed 150-160r/min, stir interval 60s after 3min, fermentation process
In pH is not adjusted.Anaerobic fermentation process continues 6-9d；
(6) fermentative acidification liquid extracts: the mixed liquor after mushroom residue fermentation in step (5) is centrifugated using centrifuge,
Centrifugal speed is 15000r/min, supernatant, that is, VFA fermentation liquid after being centrifuged 20-30min.
3. beneficial effects of the present invention
The beneficial effects of the present invention are using white-rot fungi pre-process, after pretreatment can Selective lignin-degradation,
Cellulose/lignin ratio is improved, subsequent anaerobic fermentation VFA yield is promoted to promote 65%-72%.Fermentation substrate is after pre-processing
Mushroom residue, inoculum be anaerobic digester sludge, raw material sources are extensive, and anaerobic fermentation process without pH adjust, run at
This is low, added value of product is high, realizes the minimizing of mushroom residue, innoxious and recycling treatment.It is inoculated with Pleurotus Sajor
Caju (MES03464) pre-processes 6 weeks mushroom residues, carries out intermediate temperature anaerobic fermentation under conditions of initial solid content 15%, ferments
7d, VFA concentration reaches about 7352mg/L in fermentation liquid, does not pre-process mushroom residue control group VFA concentration and improves nearly 70%.The work
Skill is easy to operate, preprocessing process and anaerobic fermentation conditions are easily controllable, economic cost is cheap, has a good application prospect.
Detailed description of the invention
The distribution of variety classes fatty acid in Fig. 1 fermentation liquid.
Specific embodiment
The present invention is further described below by specific embodiment, but is not intended to limit the present invention.
Instrument used in the present invention and material are commercially available.Wherein seed sludge is derived from certain anaerobic digester, mushroom residue
It is derived from certain mushroom plantation factory.
Embodiment 1:
(1) mushroom residue of certain a certain amount of mushroom factory is taken, it is content of cellulose 12.3%, hemicellulose level 8.3%, wooden
Cellulose content 21.7% is mechanically pulverized after drying 48h in 60 DEG C of baking ovens, is sieved, particle size 2.5-1.0cm, sieving
Mushroom residue afterwards is used for subsequent pretreatment in 121 DEG C of sterilizing 30min；
(2) sterile, under room temperature, in mass ratio 0.1 is inoculated with Pleurotus Sajor Caju (MES 03464)
In the reactor equipped with sterilizing mushroom residue, inoculation volume accounted for about the 1/3 of entire reactor.Have in 25 DEG C, humidity 75%
It is cultivated 6 weeks under the conditions of oxygen.
(3) mushroom residue obtained in step (2) is taken to be put into fermentor, be added distilled water be configured to solid content be 15% it is mixed
Close liquid；Seed sludge is added into mixed liquor, mixeding liquid volume and seed sludge volatile organic content ratio are 1:2；
(4) it seals after being passed through nitrogen 5min into the fermentor of step (3), ferments under anaerobic.Control machinery stirs
Mixing revolving speed is 120r/min, controls temperature after 37 ± 2 DEG C, anaerobic fermentation 7d, and the VFA concentration of generation is maximum, is 7159.2mg/
L。
Embodiment 2:
(1) mushroom residue of certain a certain amount of mushroom factory is taken, it is content of cellulose 17.5%, hemicellulose level 6.8%, wooden
Cellulose content 24.7% is mechanically pulverized after drying 48h in 60 DEG C of baking ovens, is sieved, particle size 3.0-1.8cm, sieving
Mushroom residue afterwards is used for subsequent pretreatment in 121 DEG C of sterilizing 30min；
(2) sterile, under room temperature, in mass ratio 0.1 is inoculated with Pleurotus Sajor Caju (MES 03464)
In the reactor equipped with sterilizing mushroom residue, inoculation volume accounted for about the 1/3 of entire reactor.Have in 25 DEG C, humidity 75%
It is cultivated 6 weeks under the conditions of oxygen.
(3) mushroom residue obtained in step (2) is taken to be put into fermentor, be added distilled water be configured to solid content be 12% it is mixed
Close liquid；Seed sludge is added into mixed liquor, mixeding liquid volume and seed sludge volatile organic content ratio are 1:2；
(4) it seals after being passed through nitrogen 5min into the fermentor of step (3), ferments under anaerobic.Control machinery stirs
Mixing revolving speed is 120r/min, controls temperature after 37 ± 2 DEG C, anaerobic fermentation 7d, and the VFA concentration of generation is maximum, is 6982.6mg/
L。
Embodiment 3:
(1) mushroom residue of certain a certain amount of mushroom factory is taken, it is content of cellulose 15.2%, hemicellulose level 8.1%, wooden
Cellulose content 19.3% is mechanically pulverized after drying 48h in 60 DEG C of baking ovens, is sieved, particle size 2.7-1.6cm, sieving
Mushroom residue afterwards is used for subsequent pretreatment in 121 DEG C of sterilizing 30min；
(2) sterile, under room temperature, in mass ratio 0.1 is inoculated with Pleurotus Sajor Caju (MES 03464)
In the reactor equipped with sterilizing mushroom residue, inoculation volume accounted for about the 1/3 of entire reactor.Have in 25 DEG C, humidity 75%
It is cultivated 6 weeks under the conditions of oxygen.
(3) mushroom residue obtained in step (2) is taken to be put into fermentor, be added distilled water be configured to solid content be 15% it is mixed
Close liquid；Seed sludge is added into mixed liquor, mixeding liquid volume and seed sludge volatile organic content ratio are 1:2；
(4) it seals after being passed through nitrogen 5min into the fermentor of step (3), ferments under anaerobic.Control machinery stirs
Mixing revolving speed is 120r/min, controls temperature after 37 ± 2 DEG C, anaerobic fermentation 7d, and the VFA concentration of generation is maximum, is 7352.3mg/
L, the distribution of variety classes fatty acid is as shown in Figure 1.
Claims (3)
1. a kind of method that white-rot fungi preconditioning technique improves mushroom residue anaerobic fermentation efficiency, it is characterised in that the method packet
Include following steps:
(1) white-rot fungi culture: the present invention uses Pleurotus Sajor Caju (P.sajor-caju, BNCC230025) bacterium
Strain, aseptically, conservation accesses 3-5 block diameter 1- from slant medium in 3% malt-agar culture, with oese
The bacteria cake of 2cm is to the culture box that 1.5L sterilizing sorghum bead is housed, and sufficiently shaking culture box is uniformly distributed mycelia, room temperature decentralization
It sets about 15 days, until sorghum surface covers with white hypha；
(2) mushroom residue pre-processes: taking a certain amount of mushroom residue, dries after 48h to constant weight, be mechanically pulverized to partial size 3.0-
1.5cm is used for subsequent white-rot fungi preprocessing process；
(3) white-rot fungi pre-processes: by pretreated mushroom residue in 121 DEG C of sterilizing 30min, throwing after cooling by 0.1 mass ratio
Lengthening has the sorghum bead of mycelia into preatreating reactors, and inoculation volume accounted for about the 1/3 of entire reactor, after inoculation, fills
Point agitation mushroom residue comes into full contact with fungi with it, and sample is transferred to 25 DEG C, is cultivated 4~6 weeks under conditions of humidity 75%；
(4) seed sludge pre-processes: by anaerobically digested sludge in 105 DEG C of heating 20-30min, removing methanogen therein, makees
For the seed sludge of mushroom residue fermentation；
(5) seed sludge in pretreated mushroom residue in step (3) and step (4) mushroom residue anaerobic fermentation: is pressed into volatility
Anaerobic fermentation reactor is added in organic matter mass ratio 1:2, and inflated with nitrogen 3-5min, control reaction temperature is 37 DEG C, and mixing speed is
150-160r/min stirs interval 60s after 3min, pH is not adjusted in fermentation process, anaerobic fermentation process continues 6-9d；
(6) fermentative acidification liquid extracts: being centrifugated the mixed liquor after mushroom residue fermentation in step (5) using centrifuge, centrifugation
Speed is 12000r/min, supernatant, that is, VFA fermentation liquid after being centrifuged 30min.
2. according to method described in right 1, which is characterized in that after Pleurotus Sajor Caju is pre-processed six weeks, fermentation
It when system total solid concentration 15%, is controlled without pH, ferment 7d, and Vfa Concentration reaches about in fermentation liquid
The pretreated control group Vfa Concentration of 6982.6mg/L, less fungi improves nearly 70%.
3. according to method described in right 1, which is characterized in that volatile fatty acid is mainly by acetic acid and propionic acid group in fermentation liquid
At the content of acetic acid and propionic acid accounts for the 72%-75% of general volatile fatty acid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111892165A (en) * | 2020-07-24 | 2020-11-06 | 温州大学 | Method for improving denitrification efficiency of pig raising wastewater |
| CN113214497A (en) * | 2021-05-06 | 2021-08-06 | 西华大学 | Method for recovering lignin from enzymolysis residues of lignocellulose |
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| CN101606579A (en) * | 2009-07-03 | 2009-12-23 | 农业部环境保护科研监测所 | Straw feed of a kind of fermented by white rot fungus and preparation method thereof |
| CN103194494A (en) * | 2013-03-22 | 2013-07-10 | 哈尔滨工业大学 | Method for producing volatile fatty acids through co-fermentation of agaricus bisporus fungus chaff and surplus sludge |
| CN105861576A (en) * | 2016-04-19 | 2016-08-17 | 湖南大学 | Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation |
| CN107435053A (en) * | 2016-05-25 | 2017-12-05 | 东北林业大学 | A kind of white-rot fungi pretreatment agricultural crop straw quickly produces the fermentation process of biogas |
-
2018
- 2018-10-09 CN CN201811173049.1A patent/CN109266696A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606579A (en) * | 2009-07-03 | 2009-12-23 | 农业部环境保护科研监测所 | Straw feed of a kind of fermented by white rot fungus and preparation method thereof |
| CN103194494A (en) * | 2013-03-22 | 2013-07-10 | 哈尔滨工业大学 | Method for producing volatile fatty acids through co-fermentation of agaricus bisporus fungus chaff and surplus sludge |
| CN105861576A (en) * | 2016-04-19 | 2016-08-17 | 湖南大学 | Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation |
| CN107435053A (en) * | 2016-05-25 | 2017-12-05 | 东北林业大学 | A kind of white-rot fungi pretreatment agricultural crop straw quickly produces the fermentation process of biogas |
Non-Patent Citations (3)
| Title |
|---|
| ROUCHES E，ET AL.: "Improvement of anaerobic degradation by white-rot fungi pretreatment of lignocellulosic biomass: Areview", 《RENEWABLE AND SUSTAINABLE ENERGY REVIEWS》 * |
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| 房玮: "白腐真菌预处理促进木质纤维素厌氧", 《中国博士学位论文全文数据库 工程科技I辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111892165A (en) * | 2020-07-24 | 2020-11-06 | 温州大学 | Method for improving denitrification efficiency of pig raising wastewater |
| CN113214497A (en) * | 2021-05-06 | 2021-08-06 | 西华大学 | Method for recovering lignin from enzymolysis residues of lignocellulose |
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Application publication date: 20190125 |