CN109337818A - A kind of shellfish ingests the algae resuscitation fluid of microalgae - Google Patents
A kind of shellfish ingests the algae resuscitation fluid of microalgae Download PDFInfo
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- CN109337818A CN109337818A CN201811345279.1A CN201811345279A CN109337818A CN 109337818 A CN109337818 A CN 109337818A CN 201811345279 A CN201811345279 A CN 201811345279A CN 109337818 A CN109337818 A CN 109337818A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention belongs to algae bio technical fields.In view of the deficiencies of the prior art, the present invention provides the algae resuscitation fluid that a kind of shellfish ingests microalgae, it is characterised in that it includes following parts by weight of component: 500-1000 parts of potassium nitrate；500-1000 parts of potassium dihydrogen phosphate；200-300 parts of disodium ethylene diamine tetraacetate；100-200 parts of ferric trichloride；100-200 parts of sodium bicarbonate；Vitamin B110-20 parts；Vitamin B1210-20 parts；2-5 parts of microelement;1500-2000 parts of distilled water.Shellfish provided by the invention ingest algae algae resuscitation fluid it is safe and reliable, have no adverse effects to shellfish micro algae growth of ingesting, avoid culture solution caused by frond pollution and waste, effectively reduce shellfish hatchery industry production cost.
Description
Technical field
The invention belongs to algae bio technical fields, and in particular to a kind of shellfish ingests the algae resuscitation fluid of microalgae.
Background technique
Shellfish needs to take in a large amount of nutriment during growth and development, relies primarily on the nutrition in intake microalgae
Substance maintains to grow, therefore shellfish ingests the degree full of nutrition of algae and its breeding degree is shellfishery is concerned in recent years
Hot issue.Although traditional algae culture medium of ingesting can guarantee the basic nutrients of shellfish growth, but be unable to ensure algae
Growth and breeding efficiency, and the influence vulnerable to miscellaneous algae and disease causes frond to pollute.
CN102093957A discloses a kind of bead algae culturing liquid high in fat of promoting rapid growth.It contains bamboo acetic acid 1-
8%, Main Nutrients 4-15%, micro-nutrient 0.2-2. 5%, soil extract 0-1. 5%, water 78-94%, the Main Nutrients
For one of sodium nitrate, dipotassium hydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, potassium dihydrogen phosphate, sodium chloride or more than one
Mixture, the micro-nutrient are one of iron chloride, boric acid, copper sulphate, manganese sulfate, zinc sulfate, ammonium molybdate or one kind
The above mixture.The culture solution makees synergistic component by adding bamboo vinegar, substantially increases what the fixed carbon dioxide of algae generated
Lipid content.
CN106591131A discloses a kind of Heterotrophic culture base for marine microalgae large-scale culture, the culture medium
It is formulated as follows: nitrate 0.5~1. 5g, 2~lOmg of potassium dihydrogen phosphate, 2~lOmg of dipotassium hydrogen phosphate, ironic citrate 0.1~
Img, 0.5~2mg of epsom salt, 0.5~2mg of calcium chloride dihydrate, 5~20g of organic carbon source, filtering sea lOOOmL.It is described
Nitrate is potassium nitrate or sodium nitrate, and the organic carbon source is glucose
Or sucrose.
It is main to support in the culture solution or culture medium that configuration promotes micro algae growth by the comparative analysis to the prior art
Dividing is nitrate, 2~lOmg of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, organoiron compound etc., and open file 1 is in above-mentioned nutrient
On the basis of addition bamboo acetic acid make synergistic component, improve the lipid content that the fixed carbon dioxide of algae generates.What open file 2 formed
Formula is widely used, and target is the large-scale culture of marine microalgae.But it ingests in shellfish and finds in algae incubation, use is existing
Culture solution (culture medium) be not controlled effectively to harmful protozoan (infusorian, belly wool worm, amoeba etc.), together
When the ingest recovery of algae of shellfish is not acted on significantly, therefore how to be reached by adjusting culture solution (culture medium) pH value
To killing harmful protozoic purpose, at the same microalgae also can rapid fluid resuscitation be grown to serve as technical problem urgently to be resolved.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides the algae resuscitation fluid that a kind of shellfish ingests microalgae.Algae recovery
Vitamin B has been especially added in liquid1, vitamin B12And the microelements such as boron, manganese, zinc, it is harmful primary adjusting pH value killing
Microalgae frond is protected during animal, and frond rapid fluid resuscitation can be made, stand density quickly increases, frond bright, vigor
It is vigorous.
Technical scheme is as follows:
A kind of shellfish ingests the algae resuscitation fluid of microalgae, it is characterised in that it includes following parts by weight of component:
500-1000 parts of potassium nitrate；
500-1000 parts of potassium dihydrogen phosphate；
200-300 parts of disodium ethylene diamine tetraacetate；
100-200 parts of ferric trichloride；
100-200 parts of sodium bicarbonate；
Vitamin B110-20 parts；
Vitamin B1210-20 parts；
2-5 parts of microelement;
1500-2000 parts of distilled water.
The pH value of above-mentioned algae resuscitation fluid is 6-8.
The adjustment of above-mentioned algae resuscitation fluid pH value is carried out by adding HCL into algae solution.
Further, the microelement refers to one of boric acid, manganese chloride, zinc chloride, cobalt chloride and ammonium molybdate
Or several combinations.
Shellfish provided by the invention ingest algae algae resuscitation fluid it is safe and reliable, ingest micro algae growth without bad shadow to shellfish
It rings, avoids culture solution caused by frond pollution and waste, effectively reduce shellfish hatchery industry production cost.Added in resuscitation fluid
The vitamin B added1、Vitamin B12、Microelement effectively raises the immunity of algae, enables algae rapid fluid resuscitation, tool
There is apparent growth vigor, while the sodium bicarbonate in mother liquor is the excellent carbon source of microalgae, resuscitation fluid is by science configuration, in mother
Good pH buffer system is formed in liquid with other components, in the case where not injuring microalgae, can effectively remove protozoan,
Miscellaneous algae, miscellaneous bacteria achieve the purpose that purify algae.
Specific embodiment
Algae resuscitation fluid provided by the invention is described in detail below by embodiment.
Embodiment 1
A kind of shellfish ingests the algae resuscitation fluid of microalgae, it is characterised in that it includes following parts by weight of component:
500 parts of potassium nitrate；
1000 parts of potassium dihydrogen phosphate；
200 parts of disodium ethylene diamine tetraacetate；
200 parts of ferric trichloride；
100 parts of sodium bicarbonate；
Vitamin B120 parts；
Vitamin B1210 parts；
5 parts of boric acid;
2000 parts of distilled water.
Above-mentioned raw materials are matched to form resuscitation fluid, HCL is then added into resuscitation fluid, the pH value of resuscitation fluid is adjusted
Whole, in the case where different PH, the vigour changes of main algae and enemy are shown in Table 1.
The vigor state of the main algae of table 1 and enemy at various ph values
As shown in Table 1, when the resuscitation fluid of the proportion is when pH value is 6-7.5, a large amount of nocuousness protozoas are dead, while algae vigor is just
Often, especially when pH value is 6-7, algae is normal, and harmful protozoa is substantially dead.
The resuscitation fluid and recovery time of original resuscitation fluid to algae are compared, algae chooses flat algae and bead respectively
Ball algae, the proportion of original resuscitation fluid are as follows:
500 parts of potassium nitrate；
1000 parts of potassium dihydrogen phosphate；
200 parts of disodium ethylene diamine tetraacetate；
200 parts of ferric trichloride；
100 parts of sodium bicarbonate；
2000 parts of distilled water.
The results are shown in Table 2 for it.(wherein ten thousand/mL refers to quantity containing algae in every ML resuscitation fluid)
Table 2 is within the identical recovery time, and resuscitation fluid provided by the invention and original resuscitation fluid bring back to life quantitative comparison to algae.
Through the visible resuscitation fluid provided by the invention of upper table and original resuscitation fluid within the identical recovery time, the present invention is mentioned
The resuscitation fluid of confession can obtain greater amount of algae recovery.
Embodiment 2
A kind of shellfish ingests the algae resuscitation fluid of microalgae, it is characterised in that it includes following parts by weight of component:
1000 parts of potassium nitrate；
500 parts of potassium dihydrogen phosphate；
300 parts of disodium ethylene diamine tetraacetate；
100 parts of ferric trichloride；
200 parts of sodium bicarbonate；
Vitamin B110 parts；
Vitamin B1220 parts；
2 parts of microelement, wherein microelement by boric acid, manganese chloride, zinc chloride according to 1:1:1 proportional arrangement；
1500 parts of distilled water.
Above-mentioned raw materials are matched to form resuscitation fluid, HCL is then added into resuscitation fluid, the pH value of resuscitation fluid is adjusted
Whole, in the case where different PH, the vigour changes of main algae and enemy are shown in Table 3.
The vigor state of the main algae of table 3 and enemy at various ph values
As shown in Table 3, when the resuscitation fluid of the proportion is when pH value is 6-7.5, a large amount of nocuousness protozoas are dead, while algae vigor is just
Often, especially when pH value is 7-7.5, algae is normal, and harmful protozoa is substantially dead.
The resuscitation fluid and recovery time of the existing culture solution to algae are compared, algae chooses flat algae and bead respectively
Ball algae, the proportion of original resuscitation fluid are as follows:
1000 parts of potassium nitrate；
500 parts of potassium dihydrogen phosphate；
300 parts of disodium ethylene diamine tetraacetate；
100 parts of ferric trichloride；
200 parts of sodium bicarbonate；
1500 parts of distilled water.
The results are shown in Table 4 for it.(wherein ten thousand/mL refers to quantity containing algae in every ML resuscitation fluid)
Table 4 is within the identical recovery time, and resuscitation fluid provided by the invention and existing resuscitation fluid bring back to life quantitative comparison to algae.
Through the visible resuscitation fluid provided by the invention of upper table and original resuscitation fluid within the identical recovery time, the present invention is mentioned
The resuscitation fluid of confession can obtain greater amount of algae recovery.
Embodiment 3
A kind of shellfish ingests the algae resuscitation fluid of microalgae, it is characterised in that it includes following parts by weight of component:
750 parts of potassium nitrate；
750 parts of potassium dihydrogen phosphate；
250 parts of disodium ethylene diamine tetraacetate；
150 parts of ferric trichloride；
150 parts of sodium bicarbonate；
Vitamin B115 parts；
Vitamin B1215 parts；
3 parts of microelement, wherein microelement is by boric acid, manganese chloride, zinc chloride, cobalt chloride, ammonium molybdate according to 2:1:1:1:1's
Proportional arrangement;
1800 parts of distilled water.
Above-mentioned raw materials are matched to form resuscitation fluid, HCL is then added into algae solution, the pH value of algae solution is adjusted,
In the case where different PH, the vigour changes of main algae and enemy are shown in Table 5.
The vigor state of the main algae of table 5 and enemy at various ph values
As shown in Table 5, when the resuscitation fluid of the proportion is when pH value is 6-8, a large amount of nocuousness protozoas are dead, while algae vigor is just
Often, especially when pH value is 7.5-8, algae is normal, and harmful protozoa is substantially dead.
The resuscitation fluid and recovery time of original resuscitation fluid to algae are compared, algae chooses flat algae and bead respectively
Ball algae, the proportion of original resuscitation fluid are as follows:
750 parts of potassium nitrate；
750 parts of potassium dihydrogen phosphate；
250 parts of disodium ethylene diamine tetraacetate；
150 parts of ferric trichloride；
150 parts of sodium bicarbonate；
1800 parts of distilled water.
The results are shown in Table 6 for it.(wherein ten thousand/mL refers to quantity containing algae in every ML resuscitation fluid)
Table 6 is within the identical recovery time, and resuscitation fluid provided by the invention and existing resuscitation fluid bring back to life quantitative comparison to algae.
Through the visible resuscitation fluid provided by the invention of upper table and original resuscitation fluid within the identical recovery time, the present invention is mentioned
The resuscitation fluid of confession can obtain greater amount of algae recovery.
Claims (4)
- The algae resuscitation fluid of microalgae 1. a kind of shellfish ingests, it is characterised in that it includes following parts by weight of component:500-1000 parts of potassium nitrate；500-1000 parts of potassium dihydrogen phosphate；200-300 parts of disodium ethylene diamine tetraacetate；100-200 parts of ferric trichloride；100-200 parts of sodium bicarbonate；Vitamin B110-20 parts；Vitamin B1210-20 parts；2-5 parts of microelement;1500-2000 parts of distilled water.
- The algae resuscitation fluid of microalgae 2. a kind of shellfish according to claim 1 ingests, it is characterised in that: the algae recovery The pH value of liquid is 6-8.
- The algae resuscitation fluid of microalgae 3. a kind of shellfish according to claim 1 ingests, it is characterised in that: the algae recovery Liquid pH value is adjusted by adding HCL into algae solution.
- The algae resuscitation fluid of microalgae 4. a kind of shellfish according to claim 1 ingests, it is characterised in that: the micro member Element refers to that one or more of boric acid, manganese chloride, zinc chloride, cobalt chloride and ammonium molybdate combine.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811345279.1A CN109337818A (en) | 2018-11-13 | 2018-11-13 | A kind of shellfish ingests the algae resuscitation fluid of microalgae |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811345279.1A CN109337818A (en) | 2018-11-13 | 2018-11-13 | A kind of shellfish ingests the algae resuscitation fluid of microalgae |
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| CN109337818A true CN109337818A (en) | 2019-02-15 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205246A (en) * | 2019-07-19 | 2019-09-06 | 宁波大学 | A kind of scenedesmus obliquus high-density cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5963146A (en) * | 1982-10-01 | 1984-04-10 | Tadashi Matsunaga | Feed for cultivation of fish and shellfish |
| CN102782145A (en) * | 2010-03-03 | 2012-11-14 | 花王株式会社 | Method of producing lauric acid-containing oil or fat |
| CN108220167A (en) * | 2018-04-10 | 2018-06-29 | 中盐工程技术研究院有限公司 | Salt algae method for purifying and separating and microalgae method for purifying and separating |
-
2018
- 2018-11-13 CN CN201811345279.1A patent/CN109337818A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5963146A (en) * | 1982-10-01 | 1984-04-10 | Tadashi Matsunaga | Feed for cultivation of fish and shellfish |
| CN102782145A (en) * | 2010-03-03 | 2012-11-14 | 花王株式会社 | Method of producing lauric acid-containing oil or fat |
| CN108220167A (en) * | 2018-04-10 | 2018-06-29 | 中盐工程技术研究院有限公司 | Salt algae method for purifying and separating and microalgae method for purifying and separating |
Non-Patent Citations (4)
| Title |
|---|
| 孙春晓等: "金藻在贝类育苗中的培养与污染防治技术", 《齐鲁渔业》 * |
| 张英等: "维生素B1、B12在雨生红球藻不同培养阶段的作用研究", 《厦门大学学报（自然科学版）》 * |
| 李耀辉等: "《海洋生物饵料培养》", 31 May 1995, 中国农业出版社 * |
| 邓祥元等: "《应用微藻生物学》", 30 November 2016, 海洋出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205246A (en) * | 2019-07-19 | 2019-09-06 | 宁波大学 | A kind of scenedesmus obliquus high-density cultivation method |
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