CN109331894A - A kind of droplet detection device based on micro-fluidic chip - Google Patents
A kind of droplet detection device based on micro-fluidic chip Download PDFInfo
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- CN109331894A CN109331894A CN201811435328.0A CN201811435328A CN109331894A CN 109331894 A CN109331894 A CN 109331894A CN 201811435328 A CN201811435328 A CN 201811435328A CN 109331894 A CN109331894 A CN 109331894A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Abstract
The droplet detection device based on micro-fluidic chip that the invention discloses a kind of, including bottom plate, reference block, Z axis adjust platform, X-Y axis adjusts platform, micro-fluidic chip clamp and droplet and detects adjustment module.For users, the problem of the step of can solve micro-fluidic chip replacement cumbersome, inefficiency, micro-fluidic chip can be accurately positioned after being put into and Automatic-clamping is fixed, and is directed to the difference in thickness of chip batch, can be finely adjusted by the present invention；For present apparatus assembling and setting, it can be quickly and efficiently directed at chip detection channel and light path and detection light source and light path, improved efficiency.
Description
Technical field
The invention belongs to microfluidic arts, and in particular to a kind of droplet detection device based on micro-fluidic chip.
Background technique
It is micro-fluidic to refer to minute fluid is handled or manipulated using microchannel (having a size of tens of to hundreds of microns) that (volume is
Nanoliter arrive A Sheng) system involved in Science and Technology, be one and be related to chemistry, fluid physics, microelectronics, new material, life
The emerging cross discipline of object and biomedical engineering.Because having many characteristics, such as micromation, integrated, micro fluidic device usually quilt
Referred to as chip lab and micro-total analysis system.Micro-fluidic important feature first is that under micro-scale environments have unique fluid
Property, such as laminar flow and drop.It is micro-fluidic a series of conventional methods may be implemented to be difficult to by these unique fluid phenomenons
The micro Process of completion and microoperation.Currently, micro-fluidic be considered having in biomedical research huge development potentiality and wide
General application prospect.
For in micro-fluidic chip droplet detection, an important premise calls be droplet detection module optical path and its
The detection channel of optical device, illuminating source and micro-fluidic chip is accurately aimed at, and will be directly affected with upper-part degree of registration micro-
Drip detection range.It is followed by the cross contamination for avoiding not homogeneous detection, micro-fluidic chip is usually disposable, therefore it is required that
Micro-fluidic chip replaces that step is simple, and installation is reliable, install require after micro-fluidic chip the test point of droplet identification module with it is micro-
The detection channel of fluidic chip is accurately aimed at.
In current existing design, as the Chinese patent of Publication No. CN106582911A proposes a kind of micro-fluidic chip
Fixture comprising pedestal is rotatably connected with pedestal and forms two flip lids of grasping part with base engagement respectively, will turn over
The retention mechanism being fixed on pedestal is covered, pedestal offers the chip slot for placing micro-fluidic chip, chip slot and two folders
Space connection is held, there is micro-fluidic chip one end of liquid outlet to protrude into another grasping part, and micro-fluidic chip is held on two
Between a flip lid；When micro-fluidic chip is installed, 4 fasteners are opened, then dig 2, then by micro-fluidic chip
Be put into chip slot, rear cover close 2 flip lids, finally 4 fastener mechanisms are locked；When needing replacing micro-fluidic chip, by 4
A retention mechanism is opened, and then digs 2, then by original micro-fluidic chip taking-up, and by new micro-fluidic chip
Be put into chip slot, finally repeatedly chip be packed into the step of.The Chinese patent of another Publication No. CN205761267U proposes
A kind of micro-fluidic chip clamp, using the method for lifting platform instead of the fixed micro-fluidic chip of fastener, which includes first
Rectangular frame, second rectangular frame and lifting platform, lifting platform are arranged in first rectangular frame, second rectangular frame activity installation
In first rectangular frame, second rectangular frame includes four L-type supports, and four L-type support head and the tail are sequentially connected, and connected two
It is connected by extensible member between a L-type support；In use, lifting platform is placed in first rectangular frame, second is then opened
Rectangular frame, then chip is placed on lifting platform, second rectangular frame rotation is covered in first rectangular frame later, it is fixed
Good second rectangular frame adjusts the height of lifting platform finally to realize the clamping of chip.
In the description of above-mentioned patented technology, micro-fluidic chip requires to operate multiple steps when being fixed on chip fixture
Suddenly, relatively cumbersome, and micro-fluidic chip can only be put into chip slot from top to bottom, such as mount a device onto instrument, operation is opposite
It is difficult.In droplet detection adjustment module, generally require to adjust detection light source relative to the position of light path and chip detection
Position of the runner relative to light path is directed at light source with optical path, usually relies on the machining accuracy of part to reach, but i.e.
Meet machining accuracy, rigging error still remains.In batch production, the consistency after being assembled using the above method is relatively difficult to ensure
Card, it is inconvenient to adjust；In use, can not fast be adjusted if the micro-fluidic chip thickness of different batches is variant with upper fixture
The position of light source is unable to reach the requirement of instrument so that detection accuracy be caused to be deteriorated.
Summary of the invention
In view of above-mentioned, the present invention provides a kind of, and the droplet detection device based on micro-fluidic chip for users can
With the cumbersome, inefficiency of the step of solving the problems, such as micro-fluidic chip replacement, micro-fluidic chip can be accurately positioned simultaneously after being put into
Automatic-clamping is fixed, and is directed to the difference in thickness of chip batch, can be finely adjusted；For present apparatus assembling and setting,
It can be quickly and efficiently directed at chip detection channel and light path and detection light source and light path, improved efficiency.
A kind of droplet detection device based on micro-fluidic chip, including bottom plate, Z axis adjust platform, X-Y axis adjusts platform,
Micro-fluidic chip, chip fixture, droplet detection adjustment module and two reference blocks；Wherein, micro-fluidic chip is installed on chip
The slim leaf spring of side pressure and upper ironed template spring for the micro-fluidic chip that is fixedly clamped, base are provided on fixture, on chip fixture
Quasi- block is set on bottom plate, and Z axis is adjusted platform and is fixedly connected by fastener with bottom plate on the basis of a wherein reference block, X-Y
Axis is adjusted platform and is fixedly connected by fastener with bottom plate on the basis of another reference block, and chip fixture is installed on by fastener
Z axis is adjusted on platform, and droplet detects adjustment module and is installed on X-Y axis adjusting platform by fastener, also right on chip fixture
Claim installation there are two droplet identification module, droplet, which detects, is equipped with two axis adjusting platform in adjustment module.
Further, it is offered on the bottom plate and passes through fastener with groove at reference block matched two, two reference blocks
It is respectively arranged in corresponding groove；The setting of groove and reference block at above-mentioned two ensure that Z axis adjusts platform and X-Y axis tune
It is parallel after section stage+module.
Further, the chip fixture includes fixture base, the slim leaf spring of two panels side pressure and the ironed template of two on pieces
Spring；Fixture base is installed on Z axis by fastener and adjusts on platform, and the core fixed for micro-fluidic chip is offered in fixture base
Piece holding tank, fixture base rear side are opened up there are two notch, and the slim leaf spring of two panels side pressure is respectively arranged in correspondence by fastener
Notch in；The ironed template spring of two on pieces is installed on the right side of fixture base by fastener, and fixture base front and rear sides are symmetrical
Card slot is offered, two droplet identification modules are respectively arranged in corresponding card slot by fastener；Distinguished by slim leaf spring
Chip is compressed from sides and top, ensure that chip is in stationary state, meet test request, droplet identification module card slot
The module can be easily installed in place by setting.
Further, the chip holding tank is slightly wider than the width of micro-fluidic chip, is open type on the left of chip holding tank
And left end is equipped with guiding chamfering, right side is equipped with benchmark rib, so that micro-fluidic chip is easy to be inserted into chip slot and pacifies
It is attached to position.
Further, the droplet identification module is used for the identification of droplet type comprising modular substrate, two LED lights
Source, two pieces of photocells and cover board；Two pairs of embedment holes are offered above modular substrate and separately below, and two LED light sources pass through
Fastener is embedded at respectively in a pair of of embedment hole above modular substrate, and two pieces of photocells are embedded at module by fastener respectively
In a pair of of embedment hole below pedestal, the corresponding runner of micro-fluidic chip is aligned with two photocells respectively, and cover board passes through fastening
Part and modular substrate are cooperatively connected；It is additionally provided with two groups of waist type shape optical channels on modular substrate, the waist type shape optical channel is located at pair
Between the LED light source and photocell answered, the width of waist type shape optical channel is greater than the width of flow path of micro-fluidic chip, but is less than photoelectricity
The width of pond photosurface, the runner of the long side of waist type shape optical channel perpendicular to micro-fluidic chip.
Further, offer escape groove on the modular substrate, escape groove be centrally placed in LED light source and photocell it
Between, evacuation groove width is more bigger than micro-fluidic chip thickness, so that being exactly in LED light source and photocell after micro-fluidic chip insertion
Between.
Further, the droplet detection adjustment module includes droplet detection adjustment module pedestal, optical fibre reel, control
Circuit board, detection circuit board, feed circuit plate, light splitting pedestal, laser and outer cover；Droplet detection adjustment module pedestal passes through
Fastener is installed on X-Y axis and adjusts on platform, and the holding tank fixed for light splitting pedestal is provided on droplet detection adjustment module pedestal,
Light splitting pedestal is placed in the holding tank by fastener, is divided on pedestal and is provided with groove, and it is logical with 45 degree of incidence angles to be divided eyeglass
It crosses dispensing to be fixedly installed in the groove, laser is installed on two axis and adjusts on platform；Two axis adjust platform and are set by fastener
In on droplet detection adjustment module pedestal, control circuit board is installed on droplet by support and fastener and detects adjustment module pedestal
Front side, detection circuit board are installed in the holding tank A on rear side of droplet detection adjustment module pedestal, and feed circuit plate is installed on droplet
In the holding tank B for detecting adjustment module base end face, offers on droplet detection adjustment module pedestal and detected for micro-fluidic chip
Hold the groove of insertion；Location hole, the central point and light splitting eyeglass of two location holes are offered in holding tank A and holding tank B respectively
Central point is in the same plane and the plane is parallel to droplet detection adjustment module chassis left side face；Droplet detection is adjusted
Light path channel and feedback light path channel, the location hole in light path channel and holding tank A are also provided on module base
It is coaxial and through holding tank locating for light splitting pedestal；Feedback light path channel it is coaxial with the location hole in holding tank B and through
Droplet detects adjustment module pedestal；Optical fibre reel then passes through metal plate connector and the support of control circuit board connects；Outer cover is logical
Fastener connect cover is crossed on the outside of entire droplet detection adjustment module.
Further, two axis adjusts platform for the adjusting to laser positions comprising X-axis adjustment fixing plate,
Regulating base board, X-axis adjust screw, Z axis adjusts screw and laser fixed plate；X-axis adjustment fixing plate is installed by fastener
Adjustment module base right side face is detected in droplet, regulating base board bottom surface is convexly equipped with gib block and correspondence and droplet detect adjustment module
The first recessed guide groove matches on pedestal；Opening waist type groove is offered on X-axis adjustment fixing plate adjusts screw card for X-axis
Enter, regulating base board right side is equipped with threaded hole, adjusts screw by the threaded hole and X-axis and connect with X-axis adjustment fixing plate, is used for
Two axis adjust the adjusting of platform X-direction, detect adjustment module pedestal with droplet by fastener after the completion of adjusting and are fixedly connected；It is micro-
It offers opening waist type groove on drop detection adjustment module pedestal to be caught in for Z axis adjusting screw, projection on the right side of laser fixed plate
There is gib block and the second guide groove that correspondence is recessed with regulating base board left side matches；Laser fixed plate upper side offers spiral shell
Pit, laser fixed plate adjust screw by the threaded hole and Z axis and connect with regulating base board, adjust the platform side Z for two axis
To adjusting, adjust after the completion of be fixedly connected by fastener with regulating base board；Mounting hole, laser are provided in laser fixed plate
Device is fixed in the mounting hole by fastener installation；Laser can effectively be adjusted by adjusting platform by two axis, so that inspection
Light source is surveyed to be aligned with light path.
Further, lengthening adjusting rod is installed on the X-axis adjusting knob of the X-Y axis adjusting platform, this, which is lengthened, is adjusted
Bar can be convenient finger and hold to adjust the alignment of chip detection channel and light path.
In installation and debugging, by the chip slot of micro-fluidic chip insertion chip fixture, light source laser is opened, is adjusted first
Whole detection light source and light path, the X-axis that two axis of rotation adjust platform adjusts screw, Z axis adjusts screw, until the light of detection light source
Beam and light path channel alignment, then tightening fastener parts；It then adjusts chip detection channel to be aligned with light path, adjust
Whole Z axis adjusts platform, X-Y axis adjusts the adjusting knob of platform, until the light beam of light source in light path passes through chip detection channel
?；Above-mentioned steps need to only be adjusted in assembling and setting once, without operation when user uses.
It, only need to be by micro-fluidic chip along the direction that chip be inserted into insertion when chip in the operation of user's actual test
In the chip slot of chip fixture, chip can be fixed automatically by the slim leaf spring of side pressure and upper ironed template spring, simultaneously
Droplet identification module is completed to be aligned with the alignment of corresponding chip runner and chip detection channel and light path；Replace chip
When, first micro-fluidic chip is extracted out out of chip fixture chip slot along the direction that chip is extracted out, then new chip is inserted into core
In the chip slot of plate clamp, the replacement of chip can be completed, such as the chip thickness of replacement is variant with before, then needs to finely tune Z
The adjusting knob that axis adjusts platform can be completed.
In summary, the present invention for users, can solve the cumbersome, inefficiency of the step of micro-fluidic chip is replaced
The problem of, micro-fluidic chip can be accurately positioned after being put into and Automatic-clamping is fixed, and is directed to the difference in thickness of chip batch,
It can be finely adjusted；For present apparatus assembling and setting, it can quickly and efficiently be directed at chip detection channel and light path
And light source and light path are detected, it improves efficiency.
Detailed description of the invention
Fig. 1 is the overall structure diagram of droplet detection device of the present invention.
Fig. 2 is the structural schematic diagram of chip fixture in apparatus of the present invention.
Fig. 3 is the structural schematic diagram of droplet identification module in apparatus of the present invention.
Fig. 4 is the cross-sectional view of droplet identification module in apparatus of the present invention.
Fig. 5 is the structural schematic diagram that droplet detects adjustment module in apparatus of the present invention.
Fig. 6 is the structural schematic diagram that two axis adjust platform in apparatus of the present invention.
In figure: 100-bottom plates, 110-reference blocks, 120-X-Y axis adjust platform, and 121-lengthen adjusting rod, 130-Z
Axis adjusts platform, 200-chip fixtures, 210-droplet identification modules, 211-droplet identification module pedestals, 212-waist type shapes
Optical channel A, 213-waist type shape optical channel B, 214-cover boards, 215-LED light source B, 216-LED light source A, 217-photocells
B, 218-photocell A, 219-escape groove, 220-chip fixture pedestals, the slim leaf spring of 230-side pressures, 240-upper ironed types
Leaf spring, 250-micro-fluidic chips, 251-micro-fluidic chips detect section, 252-connectors, 253-liquid pipes, the detection of 300-droplets
Adjustment module, 310-droplets detect adjustment module pedestal, the 311-the first guide groove, 320-light splitting pedestals, 330-two axis tune
Section platform, 331-regulating base boards, 332-X-axis adjustment fixing plates, 333-X-axis adjusting screw, 334-laser fixed plates,
335-Z axis adjust screw, 336-laser mounting holes, the 337-the second guide groove, 350-control panel pedestals, 360-metal plates
Connector, 370-optical fibre reels, 380-lasers.
Specific embodiment
In order to more specifically describe the present invention, with reference to the accompanying drawing and specific embodiment is to technical solution of the present invention
It is described in detail.
As shown in Figure 1, the droplet detection device in present embodiment based on micro-fluidic chip, including bottom plate 100, Z axis tune
Save platform 130, X-Y axis adjust platform 120, micro-fluidic chip 250, chip fixture 200, droplet detection adjustment module 300 and
Two reference blocks 110；Wherein, micro-fluidic chip 250 is installed on chip fixture 200, and reference block 110 is set on bottom plate 100,
Z axis is adjusted platform 130 and is fixedly connected by fastener with bottom plate 100 on the basis of a wherein reference block 110, and X-Y axis adjusts flat
Platform 120 is fixedly connected by fastener with bottom plate 100 on the basis of another reference block 110, and chip fixture 200 is pacified by fastener
It is adjusted on platform 130 loaded on Z axis, droplet detects adjustment module 300 and is installed on X-Y axis adjusting platform 120 by fastener, core
It is also symmetrically installed on plate clamp 200 there are two droplet identification module 210, droplet detects and is equipped with two axis tune in adjustment module 300
Save platform.
As shown in Fig. 2, chip fixture 200 includes chip fixture pedestal 220, the slim leaf spring 230 of side pressure, upper ironed template spring
240；The slim leaf spring 230 of two panels side pressure is spaced apart to be mounted on 220 trailing flank of chip fixture pedestal, the ironed template of two on pieces
Spring 240 is spaced apart to be mounted on 220 right side of chip fixture pedestal, and micro-fluidic chip 250 horizontal can be inserted into from left to right
The chip holding tank of chip fixture, there is multiple connectors 252 and liquid pipe 253 on micro-fluidic chip 250, and micro-fluidic chip detects section
251 are located at the right end of micro-fluidic chip 250.
As shown in Figure 3 and Figure 4, droplet identification module 210 include droplet identification module pedestal 211, LED light source B 215,
LED light source A216, photocell B 217, photocell A218；LED light source (abbreviation of LED light source B 215 and LED light source A216, under
It is embedded in corresponding embedment hole respectively with photocell (abbreviation of photocell B 217 and photocell A218, similarly hereinafter) together), LED light
There are escape groove 219 between source and photocell, so that droplet identification module is not influenced the insertion of chip while assurance function, keep away
Make the width of slot 219 more bigger than the thickness of micro-fluidic chip；There are waist type shape optical channel 212, this waist between LED light source and photocell
Type shape optical channel width is greater than the width of micro-fluidic chip runner, but is less than the width of photocell photosurface.
As shown in figure 5, droplet detection adjustment module 300 include droplet detection adjustment module pedestal 310, light splitting pedestal 320,
Two axis adjust platform 330, control panel pedestal 350, metal plate connecting plate 360, optical fibre reel 370 and laser 380；It is divided pedestal
320 are mounted in the light splitting pedestal holding tank of droplet detection adjustment module pedestal 310, and two axis adjust platform 330 and are mounted on droplet
The upper plane of adjustment module pedestal 310 is detected, control panel pedestal 350 is mounted on droplet detection 310 leading flank of adjustment module pedestal,
Optical fibre reel 370 is fixed on control panel pedestal 350 by metal plate connector 360.
As shown in fig. 6, it includes regulating base board 331, X-axis adjustment fixing plate 332, X-axis adjusting screw that two axis, which adjust platform 330,
333, laser fixed plate 334, Z axis adjust screw 335；Gib block and the droplet detection of the bottom surface projection of regulating base board 331 are adjusted
The first guide groove 311 cooperation recessed on module base 310 is saved, X-axis is played and adjusts guiding role, X-axis adjustment fixing plate 332 is fixed
310 right edge of adjustment module pedestal is detected in droplet, offers opening waist type groove thereon, X-axis can be caught in and adjust screw 333；It adjusts
It saves 331 right side of bottom plate and is equipped with threaded hole, the end of thread that X-axis adjusts screw 333 is screwed into the threaded hole, is twisted X-axis and is adjusted screw
The X of 333 adjustable two shaft platforms to；The gib block of the right side projection of laser fixed plate 334 and 331 left side of regulating base board
Recessed the second guide groove 337 cooperation plays Z axis and adjusts guiding role, and plane offers opening waist type groove on regulating base board 331,
Z axis can be caught in and adjust screw 335；334 upper side of laser fixed plate offers threaded hole, and the end of thread that Z axis adjusts screw 335 is twisted
Enter in the threaded hole, twists Z axis and adjust the Z-direction that two shaft platforms are adjusted in screw 335.Laser 380 is mounted on laser fixed plate
In 334 laser mounting hole 336.
The working principle and technical effect of present embodiment are as follows:
In installation and debugging, micro-fluidic chip 250 is inserted into the chip slot of chip fixture 200, opens light source laser
380, first adjustment detection light source and light path, the X-axis that two axis of rotation adjust platform 330 adjust screw 333, adjust Z axis tune
Screw 335 is saved, to the light beam and light path channel alignment for detecting light source, then tightening fastener parts；Then adjust chip
Detection channel is aligned with light path, and adjustment Z axis adjusts platform 130, X-Y axis adjusts the adjusting knob of platform 120, until detection light
The light beam of light source of road passes through chip detection channel；Above-mentioned steps need to only adjust primary, user's use in assembling and setting
Shi Wuxu operation.
In the operation of user's actual test, when chip, micro-fluidic chip 250 need to only be inserted along the direction that chip is inserted into
It, can be automatically by the slim leaf spring 230 of side pressure and upper ironed template spring 240 by chip 250 in the chip slot for entering chip fixture 200
It is fixed, is completed at the same time droplet identification module 210 and the alignment of corresponding chip runner and chip detection channel and detection light
Road alignment；When replacing chip, first micro-fluidic chip 250 is taken out out of chip fixture 200 chip slot along the direction that chip is extracted out
Out, then by the chip slot of new chip insertion chip fixture 200, the replacement of chip can be completed, such as the chip thickness of replacement
Variant with before, then the adjusting knob for needing fine tuning Z axis to adjust platform 130 can be completed.
In summary, the present invention for users, can solve the cumbersome, inefficiency of the step of micro-fluidic chip is replaced
The problem of, micro-fluidic chip can be accurately positioned after being put into and Automatic-clamping is fixed, and is directed to the difference in thickness of chip batch,
It can be finely adjusted；For present apparatus assembling and setting, it can quickly and efficiently be directed at chip detection channel and light path
And light source and light path are detected, it improves efficiency.
The above-mentioned description to embodiment is for that can understand and apply the invention convenient for those skilled in the art.
Person skilled in the art obviously easily can make various modifications to above-described embodiment, and described herein general
Principle is applied in other embodiments without having to go through creative labor.Therefore, the present invention is not limited to the above embodiments, ability
Field technique personnel announcement according to the present invention, the improvement made for the present invention and modification all should be in protection scope of the present invention
Within.
Claims (9)
1. a kind of droplet detection device based on micro-fluidic chip, it is characterised in that: adjust platform, X-Y axis including bottom plate, Z axis
Adjust platform, micro-fluidic chip, chip fixture, droplet detection adjustment module and two reference blocks；Wherein, micro-fluidic chip is pacified
Loaded on the slim leaf spring of side pressure and upper ironed type being provided on chip fixture, on chip fixture for the micro-fluidic chip that is fixedly clamped
Leaf spring, reference block are set on bottom plate, and Z axis is adjusted platform and connected on the basis of a wherein reference block by the way that fastener and bottom plate are fixed
It connects, X-Y axis is adjusted platform and is fixedly connected by fastener with bottom plate on the basis of another reference block, and chip fixture passes through fastener
It is installed on Z axis to adjust on platform, droplet detects adjustment module and is installed on X-Y axis adjusting platform by fastener, chip fixture
On be also symmetrically installed there are two droplet identification module, two axis are installed in droplet detection adjustment module and adjust platform.
2. droplet detection device according to claim 1, it is characterised in that: offered on the bottom plate and benchmark Block- matching
Two at groove, two pieces of reference blocks are respectively arranged in corresponding groove by fastener.
3. droplet detection device according to claim 1, it is characterised in that: the chip fixture includes fixture base, two
The slim leaf spring of block side pressure and two blocks of upper ironed template springs；Fixture base is installed on Z axis by fastener and adjusts on platform, fixture
The chip holding tank fixed for micro-fluidic chip is offered on pedestal, opens up that there are two notch, two pieces of side pressures on rear side of fixture base
Slim leaf spring is respectively arranged in corresponding notch by fastener；Two blocks of upper ironed template springs are installed on fixture by fastener
The right side of pedestal, fixture base front and rear sides symmetrically offer card slot, and two droplet identification modules are installed respectively by fastener
In in corresponding card slot.
4. droplet detection device according to claim 3, it is characterised in that: the chip holding tank is slightly wider than micro-fluidic core
The width of piece, chip holding tank left side is open type and left end is equipped with guiding chamfering, and right side is equipped with benchmark rib.
5. droplet detection device according to claim 1, it is characterised in that: the droplet identification module is used for droplet type
Identification comprising modular substrate, two LED light sources, two pieces of photocells and cover board；It opens above modular substrate and separately below
Equipped with two pairs of embedment holes, two LED light sources are embedded in a pair of of embedment hole above modular substrate respectively by fastener, and two pieces
Photocell is embedded in a pair of of embedment hole below modular substrate respectively by fastener, the corresponding runner difference of micro-fluidic chip
It is aligned with two photocells, cover board is cooperatively connected by fastener and modular substrate；Two groups of waist type shapes are additionally provided on modular substrate
Optical channel, between corresponding LED light source and photocell, the width of waist type shape optical channel is greater than micro- the waist type shape optical channel
The width of flow path of fluidic chip, but it is less than the width of photocell photosurface, the long side of waist type shape optical channel is perpendicular to micro-fluidic core
The runner of piece.
6. droplet detection device according to claim 5, it is characterised in that: escape groove is offered on the modular substrate,
Escape groove is centrally placed between LED light source and photocell, and evacuation groove width is more bigger than micro-fluidic chip thickness, so that micro-fluidic core
It is exactly between LED light source and photocell after piece insertion.
7. droplet detection device according to claim 1, it is characterised in that: the droplet detection adjustment module includes droplet
Detect adjustment module pedestal, optical fibre reel, control circuit board, detection circuit board, feed circuit plate, light splitting pedestal, laser
And outer cover；Droplet detects adjustment module pedestal and is installed on X-Y axis adjusting platform by fastener, and droplet detects adjustment module
The holding tank fixed for light splitting pedestal is provided on pedestal, light splitting pedestal is placed in the holding tank by fastener, is divided pedestal
On be provided with groove, light splitting eyeglass is fixedly installed in the groove with 45 degree of incidence angles by dispensing, and laser head is installed on two axis tune
It saves on platform；Two axis adjust platform and are placed on droplet detection adjustment module pedestal by fastener, and control circuit board passes through support
And fastener is installed on front side of droplet detection adjustment module pedestal, after detection circuit board is installed on droplet detection adjustment module pedestal
In the holding tank A of side, feed circuit plate is installed in the holding tank B of droplet detection adjustment module base end face, and droplet detection is adjusted
The groove for the insertion of micro-fluidic chip test side is offered on section module base；It is fixed to offer respectively in holding tank A and holding tank B
Position hole, the central point of two location holes is in the same plane with the central point for being divided eyeglass and the plane is parallel to droplet inspection
Survey adjustment module chassis left side face；Light path channel is also provided on droplet detection adjustment module pedestal and feedback light path is logical
Road, light path channel it is coaxial with the location hole in holding tank A and through light splitting pedestal locating for holding tank；Feedback light path is logical
Road is coaxial with the location hole in holding tank B and detects adjustment module pedestal through droplet；Optical fibre reel is then connected by metal plate
Fitting and the support of control circuit board connect；Outer cover covers in entire droplet by fastener connection and detects on the outside of adjustment module.
8. droplet detection device according to claim 7, it is characterised in that: two axis adjusts platform and is used for laser
The adjusting of position comprising X-axis adjustment fixing plate, regulating base board, X-axis adjust screw, Z axis adjusts screw and laser is fixed
Plate；X-axis adjustment fixing plate is installed on droplet by fastener and detects adjustment module base right side face, and regulating base board bottom surface is convexly equipped with
Gib block and correspondence detect the first guide groove recessed on adjustment module pedestal with droplet and match；It is opened on X-axis adjustment fixing plate
It adjusts screw for X-axis equipped with opening waist type groove to be caught in, regulating base board right side is equipped with threaded hole, passes through the threaded hole and X-axis
It adjusts screw to connect with X-axis adjustment fixing plate, the adjusting of platform X-direction is adjusted for two axis, pass through fastener after the completion of adjusting
It is fixedly connected with droplet detection adjustment module pedestal；Regulating base board offers opening waist type groove and is caught in for Z axis adjusting screw, swashs
The second guide groove that light device fixed plate right side is convexly equipped with gib block and correspondence is recessed with regulating base board left side matches；Laser
Fixed plate upper side offers threaded hole, and laser fixed plate adjusts screw by the threaded hole and Z axis and connect with regulating base board,
The adjusting of platform Z-direction is adjusted for two axis, is fixedly connected by fastener with regulating base board after the completion of adjusting；Laser is fixed
Mounting hole is provided on plate, laser is fixed in the mounting hole by fastener installation.
9. droplet detection device according to claim 1, it is characterised in that: the X-axis that the X-Y axis adjusts platform adjusts rotation
Lengthening adjusting rod is installed on button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811435328.0A CN109331894B (en) | 2018-11-28 | 2018-11-28 | Micro-droplet detection device based on micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (1)
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| CN114453044A (en) * | 2022-02-24 | 2022-05-10 | 中国科学院苏州生物医学工程技术研究所 | Micro-fluidic chip clamping device |
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| CN2501046Y (en) * | 2001-10-31 | 2002-07-17 | 东北大学 | Miniflow controlled chip analysis semiconductor laser induced fluorescence detector |
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| CN207352238U (en) * | 2017-10-20 | 2018-05-11 | 贰陆光学（苏州）有限公司 | A kind of optical axis means for correcting |
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| CN114453044B (en) * | 2022-02-24 | 2023-08-22 | 中国科学院苏州生物医学工程技术研究所 | Micro-fluidic chip clamping device |
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| CN109331894B (en) | 2021-02-26 |
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