CN109330740B - Method for reducing permeability of artificial blood vessel - Google Patents
Method for reducing permeability of artificial blood vessel Download PDFInfo
- Publication number
- CN109330740B CN109330740B CN201811087211.8A CN201811087211A CN109330740B CN 109330740 B CN109330740 B CN 109330740B CN 201811087211 A CN201811087211 A CN 201811087211A CN 109330740 B CN109330740 B CN 109330740B
- Authority
- CN
- China
- Prior art keywords
- collagen
- tubular fabric
- polycaprolactone
- polycaprolactone solution
- blood vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
Abstract
The invention relates to a method for reducing the permeability of an artificial blood vessel, belonging to the technical field of biomedical engineering. The invention combines centrifugal spinning and weaving in spinning, firstly prepares tubular fabric through weaving, and then forms a nanofiber membrane with a compact structure by centrifugal spinning of collagen and polycaprolactone solution to be attached to and permeate into the tubular fabric, so that a vascular wall with a compact structure is formed. The prepared artificial blood vessel avoids the defects that micropores exist among yarns of the artificial blood vessel prepared by single weaving and the blood vessel is easy to bleed due to no adhesion, and meanwhile, collagen and polycaprolactone are added into the outer layer of the blood vessel by a centrifugal spinning technology, so that the capability of the artificial blood vessel for overcoming deformation is improved, the collagen is also favorable for promoting cell differentiation, and the bioactivity of the artificial blood vessel is improved.
Description
Technical Field
The invention belongs to the technical field of biomedical engineering, and particularly relates to a method for reducing the permeability of an artificial blood vessel.
Background
With the improvement of living conditions of human beings, the incidence rate of vascular diseases is increasing, and one of effective means for treating the vascular diseases is blood vessel transplantation. However, since the number of allogeneic blood vessels used for transplantation is rare and cannot meet medical requirements, the artificial blood vessels become the most suitable substitute. The 20 th century and the 50 s. Researchers have begun a history of manufacturing textile-type artificial blood vessels. At present, the textile type artificial blood vessel is widely applied to clinic, replaces damaged blood vessels in human bodies and obtains better medical effect.
The Chinese patent publication No. CN102920531A discloses that the publication date is 2 months and 13 days in 2013, and the invention name is: the invention discloses a method for preparing a woven artificial blood vessel by weaving, which takes polyester knitting yarns, real silk knitting yarns and polyester real silk mixed knitting yarns as warp and weft yarns to weave the woven artificial blood vessel on a weaving machine. However, the artificial blood vessel prepared by the single weaving method has larger disseminating property, is easy to limit the elasticity of the artificial blood vessel, and has micropores and no adhesion among yarns, so that the yarns are easy to bleed, and are not beneficial to clinical application.
The Chinese patent publication No. CN105232184A discloses 2016, 1, 13, and has the name: the invention discloses an artificial blood vessel and a preparation method thereof and a nozzle device, and discloses the preparation method of the artificial blood vessel. Electrostatic spinning is also difficult to use on a large scale due to its low efficiency, high voltage static, solution conductivity, and other disadvantages.
Disclosure of Invention
The invention aims to provide a method for reducing the permeability of an artificial blood vessel, and the specific technical scheme of the invention is as follows:
a method for reducing the permeability of an artificial blood vessel, the preparation method comprises the following steps:
a. preparation of collagen/polycaprolactone solution
Dissolving collagen/polycaprolactone in hexafluoroisopropanol, wherein the mass fraction of the solution is 10-20%, and stirring at the speed of 200-800 r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
Wherein the ratio of two solutes in the solution, namely collagen and polycaprolactone, is 3: 7-7: 3;
b. preparation of artificial blood vessel
Weaving tubular fabric on a loom, fixing the tubular fabric on a cylindrical die, placing the cylindrical die on a winding machine, and enabling the rotating speed of a main shaft of the winding machine to be 50-100 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the outer surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04-0.1 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000-7000 r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10-20 cm, and the thickness of a centrifugal spinning nano film is 0.1-0.5 mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
The yarn used by the tubular fabric is a blended material formed by one or more of terylene, spandex, silk or cotton;
the thickness of the yarn used by the tubular fabric is 10-100D;
the surface layer and the inner layer of the tubular fabric are any one of plain weave, twill weave or satin weave;
the diameter of the tubular fabric is 5-10 mm;
the warp density of the tubular fabric is 100-600 pieces/5 cm, and the weft density is 100-600 pieces/5 cm;
the cylindrical mold may be a metal mold or a polyester or ceramic or polytetrafluoroethylene mold.
When the artificial blood vessel is manufactured, the artificial blood vessel is mainly prepared by combining centrifugal spinning and tubular fabrics, the artificial blood vessel prepared by a single weaving method has high dispersity, the elasticity of the artificial blood vessel is easy to limit, and micropores and no adhesion exist among yarns, so that the artificial blood vessel is easy to bleed. The centrifugal spinning technology is combined with the weaving, and the centrifugal spinning nanofiber membrane is attached to and permeates into the tubular fabric to form a vascular wall with a compact structure, so that the defect of vascular bleeding caused by gaps among yarns of the tubular fabric is avoided, and the anti-bleeding performance of the artificial blood vessel is improved.
In the centrifugal spinning, a high molecular solution is thrown out through a spinneret orifice by virtue of centrifugal force to form jet flow, and under the combined action of inertia force, viscous force and air resistance, the jet flow is rapidly attenuated and solidified to form fibers, and the fibers rapidly move towards a collector to form a composite fiber support. The method is simple, efficient and safe to operate, solves the problems of electrostatic spinning, and can prepare fiber membranes with various diameters (1-100 mu m), thicknesses and sizes by adjusting spinning parameters or prepare fiber scaffolds with different shapes by changing the shape of a receiver on the premise of considering spinnability. Due to the adoption of the preparation method, the high polymer material is stretched and arranged along a specific direction under the action of centrifugal force, and simultaneously, the collected fibers are directionally arranged along the rotating direction of the spinneret, so that the preparation method is completely different from the traditional spinning method and products obtained by electrostatic spinning.
When the artificial blood vessel is manufactured, collagen and polycaprolactone are added into the blood vessel. The collagen has a stable molecular structure, low immunogenicity and good biocompatibility, and can induce cell differentiation, promote wound healing and ensure that the artificial blood vessel maintains higher bioactivity in a human body; (ii) a Polycaprolactone is a nontoxic and harmless material with high strength and good biocompatibility. The product prepared by mixing two materials with different mechanical characteristics can make up the defects of a single material product, and the strength of the collagen material can be improved by adding a proper amount of polycaprolactone, so that the deformation resistance of the collagen material is improved.
The mass fraction of the total solute selected by the invention is 10-20%, because the total mass fraction is lower than the value, the solution viscosity is too low, continuous fibers are difficult to form in the spinning process, and the content of effective solidified fibers is too low; a total mass fraction higher than the above results in too high a solution viscosity, which may clog the spinneret during spinning and cause failure to spin fibers. The mass ratio of the selected collagen to the polycaprolactone is 3: 7-7: 3, and the reason is that the mass ratio is lower than the above value, so that the content of the collagen in the scaffold material is too low, and the effect of the collagen in subsequent application is difficult to play; the mass ratio of the collagen to the polycaprolactone is higher than the above value, so that the content of the polycaprolactone is low, and continuous fibers with good mechanical properties are difficult to form.
In the preparation of the artificial blood vessel, the diameter of the fiber is 0.1-10 μm, which is beneficial to promoting the adhesion and proliferation of cells, and the diameter of the centrifugal spinning fiber is determined by the centrifugal spinning process parameters, so the centrifugal spinning parameters are controlled to obtain the fiber with the diameter as follows: the rotating speed of the centrifugal tube is 2000-7000 r/min, the receiving distance is 10-20 cm, and the thickness of the centrifugal spinning nano film is 0.1-0.5 mm. If the thickness of the centrifugally spun nanometer film exceeds 0.5mm, the total thickness of the artificial blood vessel is increased, so that the elasticity of the artificial blood vessel is reduced, and the energy conversion is not facilitated. If the thickness of the centrifugal spinning nanometer membrane is less than 0.1mm, the anti-seepage capability of the blood vessel is easy to reduce, the phenomenon of blood seepage is easy to occur, and the danger is brought to the life of a patient.
When the artificial blood vessel is manufactured, the artificial blood vessels with different diameters can be manufactured by weaving tubular fabrics with different diameters; the artificial blood vessel with different thicknesses can be prepared by changing yarns with different thicknesses, and the thickness of the artificial blood vessel can also be changed by changing the time and the speed of centrifugal spinning.
The artificial blood vessel prepared by the invention has good elasticity and biocompatibility, and can adapt to artificial blood vessels with different calibers.
Detailed Description
The invention is further described with reference to specific examples.
A method for reducing the permeability of an artificial blood vessel, which comprises the following steps:
a. preparation of collagen/polycaprolactone solution
Dissolving collagen/polycaprolactone in hexafluoroisopropanol, wherein the mass fraction of the solution is 10-20%, and stirring at the speed of 200-800 r/min at the temperature of 20-60 ℃ for 10-18 h; after complete dissolution, obtaining a uniform and transparent collagen/polycaprolactone solution, and placing the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for defoaming for 2-6 h to obtain a uniform collagen/polycaprolactone solution;
wherein the ratio of the collagen to the polycaprolactone in the solution is 3: 7-7: 3;
b. preparation of artificial blood vessel
Weaving tubular fabric on a loom, fixing the tubular fabric on a cylindrical die, placing the cylindrical die on a winding machine, and enabling the rotating speed of a main shaft of the winding machine to be 50-100 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04-0.1 mm; b, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000-7000 r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10-20 cm, and the thickness of a centrifugal spinning nano film is 0.1-0.5 mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel;
wherein, the cylinder mould is a metal mould or a polyester mould or a ceramic mould or a polytetrafluoroethylene mould. DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Example one
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example two
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic structure of the tubular fabric is 2/1 left twill weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE III
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic structure of the tubular fabric is 3/1 right twill weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example four
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 5/3 warp surface satin weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 400 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE five
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 5/3 weft surface satin weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 300 pieces/5 cm, and the weft density is 500 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE six
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 7mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 7mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE seven
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 10mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 10mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example eight
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 50D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 400 pieces/5 cm, and the weft density is 400 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example nine
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 100D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 300 pieces/5 cm, and the weft density is 300 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example ten
a. Preparation of collagen/polycaprolactone solution
Placing 5g of collagen and 5g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE eleven
a. Preparation of collagen/polycaprolactone solution
Placing 7g of collagen and 3g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twelve
a. Preparation of collagen/polycaprolactone solution
Placing 4.5g of collagen and 10.5g of polycaprolactone into 85g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min for 10-18 h at the temperature of 20-60 ℃; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE thirteen
a. Preparation of collagen/polycaprolactone solution
Placing 6g of collagen and 14g of polycaprolactone into 80g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example fourteen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.07 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example fifteen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.1 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 4000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 15cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example sixteen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of polytetrafluoroethylene, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 7000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 20cm, and the thickness of a centrifugal spinning nano film is 0.3mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example seventeen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal iron, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.5mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
EXAMPLE eighteen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 30D spandex yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 400 pieces/5 cm, and the weft density is 400 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example nineteen
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; after complete dissolution, obtaining a uniform and transparent collagen/polycaprolactone solution, and placing the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for defoaming for 2-6 h to obtain a uniform collagen/polycaprolactone solution;
b. preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D polyester yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D silk yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty one
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D cotton yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty two
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is 10D wool yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty three
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D spandex and a strand of 10D terylene, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-four
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; after complete dissolution, obtaining a uniform and transparent collagen/polycaprolactone solution, and placing the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for defoaming for 2-6 h to obtain a uniform collagen/polycaprolactone solution;
b. preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D terylene and a strand of 20D cotton yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-five
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D spandex and a strand of 20D cotton yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 600 pieces/5 cm, and the weft density is 600 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-six
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D terylene and a strand of 20D silk yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 400 pieces/5 cm, and the weft density is 400 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-seven
a. Weaving of woven tubular structures
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving a tubular fabric on a mechanical loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D silk and a strand of 20D cotton yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 300 pieces/5 cm, and the weft density is 300 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-eight
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D silk and a strand of 20D wool yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 300 pieces/5 cm, and the weft density is 300 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Example twenty-nine
a. Preparation of collagen/polycaprolactone solution
Placing 3g of collagen and 7g of polycaprolactone into 90g of hexafluoroisopropanol, wherein the mass fraction of the solution is 10%, and stirring at the speed of 500r/min at the temperature of 20-60 ℃ for 10-18 h; and (3) obtaining a uniform and transparent collagen/polycaprolactone solution after complete dissolution, and defoaming the stirred collagen/polycaprolactone solution in a vacuum environment (0.1-0.05 MPa) for 2-6 h to obtain the uniform collagen/polycaprolactone solution.
b. Preparation of artificial blood vessel
Weaving tubular fabric on a loom, wherein the surface layer and inner layer basic tissue of the tubular fabric is 1/1 plain weave, the used yarn is formed by mixing a strand of 10D spandex and a strand of 20D wool yarn, the diameter of the tubular fabric is 5mm, the warp density of the fabric is 300 pieces/5 cm, and the weft density is 300 pieces/5 cm; fixing the tubular fabric on a cylindrical die with the diameter of 5mm, wherein the cylindrical die is made of metal steel, the cylindrical die is placed on a winding machine, and the rotating speed of a main shaft of the winding machine is 50 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04 mm; and c, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10cm, and the thickness of a centrifugal spinning nano film is 0.1mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel.
Claims (6)
1. A preparation method of an artificial blood vessel for reducing the permeability of the artificial blood vessel is characterized by comprising the following steps:
a. preparing a collagen/polycaprolactone solution, namely dissolving the collagen/polycaprolactone in hexafluoroisopropanol, wherein the mass fraction of the solution is 10-20%, and stirring the solution at a speed of 200-800 r/min for 10-18 h at the temperature of 20-60 ℃; completely dissolving to obtain uniform and transparent collagen/polycaprolactone solution, placing the stirred collagen/polycaprolactone solution into a vacuum environment, and defoaming for 2-6 h under the vacuum environment of 0.05-0.1 MPa to obtain the uniform collagen/polycaprolactone solution; wherein the ratio of the collagen to the polycaprolactone in the solution is 3: 7-7: 3;
b. preparing an artificial blood vessel, namely weaving a tubular fabric on a loom, fixing the tubular fabric on a cylindrical die, placing the cylindrical die on a winding machine, and enabling the rotating speed of a main shaft of the winding machine to be 50-100 r/min; b, dipping a proper amount of the collagen/polycaprolactone solution prepared in the step a by using a brush, and brushing the collagen/polycaprolactone solution on the outer surface of the tubular fabric, so that a thin and uniform collagen/polycaprolactone solution layer is coated on the surface of the tubular fabric, and the thickness of the collagen/polycaprolactone solution layer is 0.04-0.1 mm; b, placing the collagen/polycaprolactone solution prepared in the step a into a centrifugal tube of a centrifugal spinning instrument, wherein the rotating speed of the centrifugal tube is 2000-7000 r/min, the distance from a main shaft of a cylinder die to a spinning nozzle is 10-20 cm, and the thickness of a centrifugal spinning nano film is 0.1-0.5 mm, and taking down the cylinder die to obtain the prepared complete artificial blood vessel; wherein, the cylinder mould is a metal mould or a polyester mould or a ceramic mould or a polytetrafluoroethylene mould.
2. The method for preparing a vascular prosthesis for decreasing the permeability of vascular prosthesis according to claim 1, wherein: the yarn used by the tubular fabric is a blended material formed by one or more of terylene, spandex, silk or cotton.
3. The method for preparing a vascular prosthesis for decreasing the permeability of vascular prosthesis according to claim 1, wherein: the surface layer and the inner layer of the tubular fabric are plain weave, twill weave or satin weave.
4. The method for preparing a vascular prosthesis for decreasing the permeability of vascular prosthesis according to claim 1, wherein: the warp density of the tubular fabric is 100-600 pieces/5 cm, and the weft density of the tubular fabric is 100-600 pieces/5 cm.
5. The method for preparing a vascular prosthesis for decreasing the permeability of vascular prosthesis according to claim 1, wherein: the thickness of the yarn used by the tubular fabric is 10-100D.
6. The method for preparing a vascular prosthesis for decreasing the permeability of vascular prosthesis according to claim 1, wherein: the diameter of the tubular fabric is 5-10 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811087211.8A CN109330740B (en) | 2018-09-18 | 2018-09-18 | Method for reducing permeability of artificial blood vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811087211.8A CN109330740B (en) | 2018-09-18 | 2018-09-18 | Method for reducing permeability of artificial blood vessel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109330740A CN109330740A (en) | 2019-02-15 |
| CN109330740B true CN109330740B (en) | 2021-04-23 |
Family
ID=65305517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811087211.8A Active CN109330740B (en) | 2018-09-18 | 2018-09-18 | Method for reducing permeability of artificial blood vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109330740B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063816A (en) * | 2019-04-28 | 2019-07-30 | 吉林大学中日联谊医院 | A kind of artificial blood vessel and its manufacturing method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264349A (en) * | 2008-04-23 | 2008-09-17 | 东华大学 | Method for preparing composite artificial blood vessel stent by combined electro-spinning with knitting technique |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| WO2015134853A1 (en) * | 2014-03-06 | 2015-09-11 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Electrospinning with sacrificial template for patterning fibrous constructs |
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
| CN105310796A (en) * | 2014-06-26 | 2016-02-10 | 上海微创医疗器械（集团）有限公司 | Artificial blood vessel and preparation method thereof and needling die |
| CN106075596A (en) * | 2016-07-21 | 2016-11-09 | 南开大学 | A kind of three layers of artificial blood vessel's technology of preparing |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100364620C (en) * | 2006-08-30 | 2008-01-30 | 郑军 | Artificial blood vessel collagen pre-coagulation coating |
| CN106492272B (en) * | 2015-11-27 | 2019-07-02 | 广州迈普再生医学科技股份有限公司 | Tissue repair tunica fibrosa and its preparation method and application and tissue repair product |
| CN105543990B (en) * | 2016-01-19 | 2018-07-06 | 浙江理工大学 | A kind of centrifugal spinning preparation method of micron or nano fibrous membrane |
| CN106620877B (en) * | 2016-11-02 | 2019-05-10 | 叶川 | A kind of capillary network and preparation method thereof |
-
2018
- 2018-09-18 CN CN201811087211.8A patent/CN109330740B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264349A (en) * | 2008-04-23 | 2008-09-17 | 东华大学 | Method for preparing composite artificial blood vessel stent by combined electro-spinning with knitting technique |
| CN101507843A (en) * | 2009-03-20 | 2009-08-19 | 中国人民解放军第三军医大学 | Multi-purpose surgery biology patching material |
| WO2015134853A1 (en) * | 2014-03-06 | 2015-09-11 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Electrospinning with sacrificial template for patterning fibrous constructs |
| CN105310796A (en) * | 2014-06-26 | 2016-02-10 | 上海微创医疗器械（集团）有限公司 | Artificial blood vessel and preparation method thereof and needling die |
| CN105233345A (en) * | 2015-08-25 | 2016-01-13 | 上海交通大学医学院附属仁济医院 | Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof |
| CN106075596A (en) * | 2016-07-21 | 2016-11-09 | 南开大学 | A kind of three layers of artificial blood vessel's technology of preparing |
Non-Patent Citations (1)
| Title |
|---|
| 《纤维基仿生人造血管的制备及其研究》;冯锦旗;《中国优秀硕士学位论文全文数据库医药卫生科技辑（电子期刊）》;20180115;E080-81 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109330740A (en) | 2019-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sun et al. | Electrospun anisotropic architectures and porous structures for tissue engineering | |
| KR101328645B1 (en) | Nano/micro hybrid fiber non-woven fabric using biodegradable polymers and method for preparing the same | |
| CN103394114B (en) | A kind of preparation method of medical dressing chitosan-based superfine fiber carrier material | |
| CN101781815B (en) | Preparation method of porous fiber with controllable degradation rate for tissue engineering scaffold | |
| CN103320886B (en) | Bionic regenerated silk fibroin filament fiber and preparation method thereof | |
| CN112553785B (en) | Double-layer guided tissue regeneration membrane and preparation method thereof | |
| CN111001042B (en) | Completely degradable tissue engineering skin scaffold material and preparation method thereof | |
| CN106512087A (en) | Artificial blood vessel stent with aligned fibers and manufacturing method of artificial blood vessel stent | |
| CN107865979A (en) | A kind of three-dimensional manometer fibrous framework based on microflow control technique and electrostatic spinning technique and preparation method thereof | |
| CN106075578B (en) | A kind of PLGA three-dimensional nerve conduit and preparation method thereof | |
| KR101035870B1 (en) | Absorbable bulky multi-filament draw textured yarn, manufacturing method thereof and medical use using them | |
| CN109330740B (en) | Method for reducing permeability of artificial blood vessel | |
| CN111265721B (en) | Preparation method of electrostatic spinning double-layer artificial blood vessels with different diameters | |
| CN110124109B (en) | Artificial blood vessel stent and preparation method and application thereof | |
| CN104287869B (en) | A kind of novel nano tunica fibrosa/yarn count frame for graft of trachea and preparation method thereof | |
| CN111714704B (en) | PGS/SF electrospun artificial blood vessel and preparation method thereof | |
| Li et al. | Characterization of electrospun core/shell poly (vinyl pyrrolidone)/poly (L-lactide-co-ε-caprolactone) fibrous membranes and their cytocompatibility in vitro | |
| CN109172875A (en) | A kind of preparation method of micron and nanometer composite structure artificial blood vessel | |
| CN1730117A (en) | Peripheral nerve regeneration conduit and process for preparing the same | |
| CN111850760A (en) | Method for preparing high-orientation-degree silk fibroin nanofiber yarn by using waste silk | |
| CN109481741B (en) | Nano composite anti-adhesion hernia repair patch and preparation method thereof | |
| CN109172872A (en) | A kind of preparation method being orientated fiber reinforcement artificial blood vessel | |
| CN109331231B (en) | Preparation method of high-activity artificial blood vessel stent | |
| KR20150053371A (en) | Bone graft material for regeneration of periodontal tissue and preparation method thereof | |
| Bossard et al. | Biomaterials from chitosan processed by electrospinning |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |