CN109330740A - A method of reducing artificial blood vessel permeance property - Google Patents
A method of reducing artificial blood vessel permeance property Download PDFInfo
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- CN109330740A CN109330740A CN201811087211.8A CN201811087211A CN109330740A CN 109330740 A CN109330740 A CN 109330740A CN 201811087211 A CN201811087211 A CN 201811087211A CN 109330740 A CN109330740 A CN 109330740A
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- collagen
- tubing
- polycaprolactone
- blood vessel
- artificial blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
- A61F2/06—Blood vessels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2240/00—Manufacturing or designing of prostheses classified in groups A61F2/00 - A61F2/26 or A61F2/82 or A61F9/00 or A61F11/00 or subgroups thereof
- A61F2240/001—Designing or manufacturing processes
Abstract
The present invention relates to a kind of methods for reducing artificial blood vessel permeance property, belong to biomedical engineering technology field.The present invention by weaving centrifugal spinning and woven two methods combine, it first passes through and woven prepares tubing, then collagen and polycaprolactone solution are formed into hard-packed nano fibrous membrane by centrifugal spinning and attaches and penetrate into tubing, form it into hard-packed vascular wall.Have micropore and without bonding cause blood vessel be easy oozing of blood the shortcomings that, is avoided between its yarn of the artificial blood vessel of single woven preparation by artificial blood vessel obtained, collagen and polycaprolactone joined in blood vessel external by centrifugal spinning technology simultaneously, improve the ability that artificial blood vessel overcomes deformation, collagen is also beneficial to promote cell differentiation, improve the bioactivity of artificial blood vessel, and the method operation is relatively easy, and efficiency is higher, comparatively safe.
Description
Technical field
The invention belongs to biomedical engineering technology fields, and in particular to a kind of side for reducing artificial blood vessel permeance property
Method.
Background technique
With the raising of human lives' condition, the disease incidence of blood vessel kind disease also increasingly increases, and treatment blood vessel kind disease has
One of effect means are exactly vasotransplantation.But since the allogeneic blood vessels rare numbers for transplanting are unable to satisfy medical demand, old friend
Hematopoiesis pipe becomes most suitable substitute.From the 1950s.The manufacture that scientific research personnel has started Weave type artificial blood vessel is gone through
History.Currently, Weave type artificial blood vessel has been widely used in clinic, the blood vessel of corrupted is replaced in human body, and achieve preferably
Medical effect.
China Patent Publication No. CN102920531A, publication date are on 2 13rd, 2013, denomination of invention are as follows: Yi Zhongji
Knit the preparation method of artificial blood vessel, a kind of method by woven preparation artificial blood vessel of the disclosure of the invention, with terylene woven yarn
Line, silk braiding yarn and terylene silk mixed weaving yarn weave woven blood vessel on woven machine as warp and weft.But
It is that artificial blood vessel laddering prepared by single weaving process is larger, is easy the elasticity of limitation artificial blood vessel, and between yarn
There is micropore and be not bonded, so that it is easy oozing of blood, be unfavorable for clinical application.
China Patent Publication No. CN105232184A, publication date are on January 13rd, 2016, denomination of invention are as follows: Yi Zhongren
Hematopoiesis pipe and preparation method thereof and ejecting device, a kind of preparation method of artificial blood vessel of the disclosure of the invention pass through and cut fabric
And fabric is stitched into cylindrical shape, the outer wall of artificial blood vessel is formed, then the outer wall is put into hollow metal
In the inner cavity of pipe, so that the outer surface of the outer wall and the inner surface of the metal tube fit, then by the outer tube
Receiving end of the inner surface of wall as electrostatic spinning, carries out electrostatic spinning, and the nanofiber forms the interior of the artificial blood vessel
Layer tube wall.Electrostatic spinning also because of its low efficiency, need high-pressure electrostatic, solution to need the disadvantages of conductive that it be made to be difficult to large-scale use.
Summary of the invention
The purpose of the present invention is to provide a kind of method for reducing artificial blood vessel permeance property out, particular techniques of the invention
Scheme are as follows:
A method of artificial blood vessel permeance property being reduced, the preparation method follows the steps below:
A. collagen/polycaprolactone solution preparation
Collagen/polycaprolactone is dissolved in hexafluoroisopropanol, liquid quality fraction is 10%~20%, with 200~
The speed of 800r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
Wherein, the ratio of two kinds of solute collagens and polycaprolactone is 3:7~7:3 in solution；
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing is fixed on cylinder mold, cylinder mold is placed
On the winder, the up- coiler speed of mainshaft is 50~100r/min；Dipped with brush the collagen that is prepared in right amount through step a/
The brushing of polycaprolactone solution makes tubing outer surface coat one layer of thin and uniform collagen egg on tubing outer surface
White/polycaprolactone solution layer, with a thickness of 0.04~0.1mm；The collagen prepared through a step/polycaprolactone solution is placed in
In the centrifuge tube of centrifugal spinning instrument, centrifuge tube revolving speed is 2000~7000r/min, cylinder mold main shaft to spinning head distance
For 10~20cm, centrifugation spins nanometer film with a thickness of 0.1~0.5mm, cylinder mold is removed to get complete to what is prepared
Artificial blood vessel.
Wherein, yarn used in tubing be one in terylene or spandex or silk or cotton or in which multiple formation it is mixed
Woven material；
Thickness of yarn used in tubing is 10~100D；
Tubing table inner layer basic organization is any one in plain weave, twill-weave or satin weave；
Tubing diameter is 5~10mm；
Tubing through it is close be 100~600/5cm, filling density is 100~600/5cm；
Cylinder mold can be metal die or polyester or ceramics or Teflon mould.
When the present invention manufactures artificial blood vessel, mainly combined by centrifugal spinning and tubing to prepare artificial blood vessel,
Artificial blood vessel laddering prepared by single weaving process is larger, is easy the elasticity of limitation artificial blood vessel, and has between yarn
It micropore and is not bonded, its is made to be easy oozing of blood.Will centrifugation spinning technique and it is woven combine, centrifugation spinning nanometer fiber membrane attach and seep
Tubing is penetrated into, hard-packed vascular wall is formed it into, gap leads to angiorrhea between avoiding tubing yarn
Disadvantage improves the blood seepage-proofing performance of artificial blood vessel.
Centrifugal spinning is to throw away Polymer Solution to form jet stream through spinneret orifice by centrifugal force, and in inertia force, viscous
It under power and the collective effect of air drag, is attenuated is formed by curing fiber rapidly, and quickly move to form compound fibre to collector
Dimensional scaffold.This method is easy to operate, efficient and safe, solves the problems, such as the presence of electrostatic spinning, before taking into account spinnability
Put by adjust spinning parameter can prepare multiple diameter (1~100 μm), thickness, size tunica fibrosa or can by change connect
The shape of device is received to prepare fibrous framework of different shapes.Due to the preparation method more than using, under the influence of centrifugal force
High molecular material is stretched to be arranged along specific direction, and the fiber being collected simultaneously is aligned along spinning head direction of rotation,
Therefore it is entirely different with traditional obtained product of spinning process and electrostatic spinning.
When the present invention manufactures artificial blood vessel, collagen and polycaprolactone joined in blood vessel.Collagen-stabilized point
Minor structure, and have low immunogenicity and good biocompatibility, and collagen can Cell differentiation inducing activity, promote wound
Mouth healing, makes artificial blood vessel keep higher bioactivity in human body；；Polycaprolactone is that a kind of intensity is higher, is had fine
Biocompatibility nontoxic material.Two kinds of materials with different mechanical characteristics are mixed be prepared into product can
The shortcomings that make up homogenous material product, the strength of collagen material can be increased by adding suitable pla-pcl, improve collagen
Protein material overcomes the ability of deformation.
The selected total soluble matters mass fraction of the present invention is 10~20%, the reason is that total mass fraction can be led lower than above-mentioned value
It causes solution viscosity too low, is difficult to form continuous fiber in spinning process and effective cured fiber content is too low；Total mass fraction is high
It is too high to will lead to solution viscosity in above-mentioned value, spinneret can be blocked during the spinning process and lead to not spin fiber.It is selected
Collagen and polycaprolactone mass ratio are 3:7~7:3, the reason is that mass ratio will lead to collagen in timbering material lower than above-mentioned value
Protein content is too low, is difficult to play its effect in subsequent applications；Collagen and polycaprolactone mass ratio are higher than above-mentioned value meeting
Lead to the low continuous fiber for being difficult to form good mechanical properties of polycaprolactone content.
In the preparation of artificial blood vessel of the present invention, due to fibre diameter may advantageously facilitate at 0.1~10 μm cell docile and
Proliferation, and the diameter of centrifugal spinning fiber is determined by centrifugal spinning process parameter, therefore to obtain the fiber of diameter above, is controlled
Centrifugal spinning parameter is as follows:, centrifuge tube revolving speed is 2000~7000r/min, and receiving distance is 10~20cm, and nanometer film is spun in centrifugation
With a thickness of 0.1~0.5mm.If centrifugation spins the thickness of nanometer film beyond after 0.5mm, the overall thickness of artificial blood vessel will add
Greatly, the flexibility decrease for leading to artificial blood vessel is unfavorable for the conversion of energy.If the thickness that nanometer film is spun in centrifugation is less than 0.1mm, hold
, easily there is the phenomenon that oozing of blood, brings danger to patients ' lives in the leak-preventing ability decline for easily leading to blood vessel.
When the present invention manufactures artificial blood vessel, the people of different-diameter can be prepared by weaving the tubing of different-diameter
Hematopoiesis pipe；The artificial blood vessel of different-thickness can be prepared by changing the yarn of different thicknesses, it can also be by changing centrifugal spinning
Time and speed change the thickness of artificial blood vessel.
Artificial blood vessel elasticity and bio-compatible performance prepared by the present invention are preferable, adapt to the artificial of large and small different bores
Blood vessel.
Specific embodiment
The invention will be further described combined with specific embodiments below.
A method of artificial blood vessel permeance property being reduced, the preparation method sequentially includes the following steps:
A. collagen/polycaprolactone solution preparation
Collagen/polycaprolactone is dissolved in hexafluoroisopropanol, liquid quality fraction is 10%~20%, with 200~
The speed of 800r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution；
Wherein, the ratio of collagen and polycaprolactone is 3:7~7:3 in solution；
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing is fixed on cylinder mold, cylinder mold is placed
On the winder, the up- coiler speed of mainshaft is 50~100r/min；Dipped with brush the collagen that is prepared in right amount through step a/
The brushing of polycaprolactone solution on tubing outer surface, make tubing surface coat one layer of thin and uniform collagen/
Polycaprolactone solution layer, with a thickness of 0.04~0.1mm；The collagen prepared through a step/polycaprolactone solution is placed in centrifugation
In the centrifuge tube of spinning apparatus, centrifuge tube revolving speed is 2000~7000r/min, and cylinder mold main shaft to spinning head distance is 10
~20cm, centrifugation spin nanometer film with a thickness of 0.1~0.5mm, cylinder mold are removed to get complete artificial to what is prepared
Blood vessel；
Wherein, cylinder mold is metal die or polyester or ceramics or Teflon mould.Specific embodiment
Embodiment one
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment two
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 2/1 left twill tissue, yarn used
For 10D spun polyester thread, tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；Tubulose is knitted
Object is fixed on the cylinder mold of diameter 5mm, and cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment three
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 3/1 twill left to right tissue, yarn used
For 10D spun polyester thread, tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；Tubulose is knitted
Object is fixed on the cylinder mold of diameter 5mm, and cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Example IV
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 5/3 Warp Satin tissue, yarn used
Line is 10D spun polyester thread, and tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density is 400/5cm；By tubulose
Fabric is fixed on the cylinder mold of diameter 5mm, and cylinder mold is made of metallic steel, and cylinder mold is placed in winding
On machine, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared in right amount through step a is dipped with brush
It brushes on tubing outer surface, tubing surface is made to coat one layer thin and uniform collagen/polycaprolactone solution
Layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument
In, centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment five
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 5/3 satin tissue, yarn used
Line is 10D spun polyester thread, and tubing diameter is 5mm, fabric through it is close be 300/5cm, filling density is 500/5cm；By tubulose
Fabric is fixed on the cylinder mold of diameter 5mm, and cylinder mold is made of metallic steel, and cylinder mold is placed in winding
On machine, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared in right amount through step a is dipped with brush
It brushes on tubing outer surface, tubing surface is made to coat one layer thin and uniform collagen/polycaprolactone solution
Layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument
In, centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment six
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 7mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 7mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment seven
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 10mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 10mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment eight
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
50D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 400/5cm, filling density is 400/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment nine
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
100D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 300/5cm, filling density is 300/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment ten
A. collagen/polycaprolactone solution preparation
5g collagen and 5g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 11
A. collagen/polycaprolactone solution preparation
7g collagen and 3g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 12
A. collagen/polycaprolactone solution preparation
4.5g collagen and 10.5g polycaprolactone are placed in 85g hexafluoroisopropanol, liquid quality fraction 10%,
The stirring that the time is 10~18h is carried out under 20~60 DEG C of environment with the speed of 500r/min；It is obtained after dissolution completely uniformly thoroughly
Collagen after the completion of stirring/polycaprolactone solution is put into vacuum environment (0.1 by bright collagen/polycaprolactone solution
~0.05MPa) under 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 13
A. collagen/polycaprolactone solution preparation
6g collagen and 14g polycaprolactone are placed in 80g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 14
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.07mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 15
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.1mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument, from
Heart pipe revolving speed is 4000r/min, and cylinder mold main shaft to spinning head distance is 15cm, centrifugation spinning nanometer film with a thickness of 0.1mm,
Cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 16
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of polytetrafluoroethylene (PTFE), and cylinder mold is placed in winding
On machine, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared in right amount through step a is dipped with brush
It brushes on tubing outer surface, tubing surface is made to coat one layer thin and uniform collagen/polycaprolactone solution
Layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument
In, centrifuge tube revolving speed be 7000r/min, cylinder mold main shaft to spinning head distance be 20cm, centrifugation spinning nanometer film with a thickness of
0.3mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 17
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic iron, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.5mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 18
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
30D spandex yarn, tubing diameter are 5mm, fabric through it is close be 400/5cm, filling density is 400/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 19
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution；
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D spun polyester thread, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 20
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D silk yarn, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 21
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D cotton, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；Tubing is consolidated
It being scheduled on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, cylinder mold placed on the winder,
The up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone solution the brushing prepared in right amount through step a is dipped with brush
On tubing outer surface, tubing surface is made to coat one layer thin and uniform collagen/polycaprolactone solution layer, it is thick
Degree is 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument, from
Heart pipe revolving speed is 2000r/min, and cylinder mold main shaft to spinning head distance is 10cm, centrifugation spinning nanometer film with a thickness of 0.1mm,
Cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 22
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
10D wool yarn, tubing diameter are 5mm, fabric through it is close be 600/5cm, filling density is 600/5cm；By tubing
It is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, and cylinder mold is placed in up- coiler
On, the up- coiler speed of mainshaft is 50r/min；Collagen/polycaprolactone the solution prepared through step a in right amount is dipped with brush to apply
Brush makes tubing surface coat one layer thin and uniform collagen/polycaprolactone solution layer on tubing outer surface,
With a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in the centrifuge tube of centrifugal spinning instrument,
Centrifuge tube revolving speed be 2000r/min, cylinder mold main shaft to spinning head distance be 10cm, centrifugation spinning nanometer film with a thickness of
0.1mm removes cylinder mold to get to the complete artificial blood vessel prepared.
Embodiment 23
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D spandex and one 10D terylene mix, and tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density is
600/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, by cylinder
Mould is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；The collagen prepared in right amount through step a is dipped with brush
Albumen/polycaprolactone solution brushing makes tubing surface coat one layer of thin and uniform collagen on tubing outer surface
Albumen/polycaprolactone solution layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in centrifugation
In the centrifuge tube of spinning apparatus, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft to spinning head distance is 10cm, from
The heart spins nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 24
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution；
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D terylene and one 20D cotton mix, and tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density
For 600/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will justify
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；The glue prepared in right amount through step a is dipped with brush
Former albumen/polycaprolactone solution brushing makes tubing surface coat one layer of thin and uniform glue on tubing outer surface
Former albumen/polycaprolactone solution layer, with a thickness of 0.04mm；By the collagen prepared through a step/polycaprolactone solution be placed in from
In the centrifuge tube of heart spinning apparatus, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft to spinning head distance is 10cm,
Centrifugation spins nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 25
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D spandex and one 20D cotton mix, and tubing diameter is 5mm, fabric through it is close be 600/5cm, filling density
For 600/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will justify
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；The glue prepared in right amount through step a is dipped with brush
Former albumen/polycaprolactone solution brushing makes tubing surface coat one layer of thin and uniform glue on tubing outer surface
Former albumen/polycaprolactone solution layer, with a thickness of 0.04mm；By the collagen prepared through a step/polycaprolactone solution be placed in from
In the centrifuge tube of heart spinning apparatus, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft to spinning head distance is 10cm,
Centrifugation spins nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 26
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D terylene and one 20D silk yarn mix, and tubing diameter is 5mm, fabric through it is close be 400/5cm, latitude
Close is 400/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；It is dipped with brush and to be prepared in right amount through step a
Collagen/polycaprolactone solution brushing coats tubing surface one layer thin and uniform on tubing outer surface
Collagen/polycaprolactone solution layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in
In the centrifuge tube of centrifugal spinning instrument, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft is to spinning head distance
10cm, centrifugation spin nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 27
A. the weaving of woven tubular tissue
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D silk and one 20D cotton mix, and tubing diameter is 5mm, fabric through it is close be 300/5cm, filling density
For 300/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will justify
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；The glue prepared in right amount through step a is dipped with brush
Former albumen/polycaprolactone solution brushing makes tubing surface coat one layer of thin and uniform glue on tubing outer surface
Former albumen/polycaprolactone solution layer, with a thickness of 0.04mm；By the collagen prepared through a step/polycaprolactone solution be placed in from
In the centrifuge tube of heart spinning apparatus, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft to spinning head distance is 10cm,
Centrifugation spins nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 28
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D silk and one 20D wool yarn mix, and tubing diameter is 5mm, fabric through it is close be 300/5cm, latitude
Close is 300/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；It is dipped with brush and to be prepared in right amount through step a
Collagen/polycaprolactone solution brushing coats tubing surface one layer thin and uniform on tubing outer surface
Collagen/polycaprolactone solution layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in
In the centrifuge tube of centrifugal spinning instrument, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft is to spinning head distance
10cm, centrifugation spin nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Embodiment 29
A. collagen/polycaprolactone solution preparation
3g collagen and 7g polycaprolactone are placed in 90g hexafluoroisopropanol, liquid quality fraction 10%, with
The speed of 500r/min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；Homogeneous transparent is obtained after dissolution completely
Collagen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution.
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing table inner layer basic organization is 1/1 plain weave, and yarn used is
One 10D spandex and one 20D wool yarn mix, and tubing diameter is 5mm, fabric through it is close be 300/5cm, latitude
Close is 300/5cm；Tubing is fixed on the cylinder mold of diameter 5mm, cylinder mold is made of metallic steel, will
Cylinder mold is placed on the winder, and the up- coiler speed of mainshaft is 50r/min；It is dipped with brush and to be prepared in right amount through step a
Collagen/polycaprolactone solution brushing coats tubing surface one layer thin and uniform on tubing outer surface
Collagen/polycaprolactone solution layer, with a thickness of 0.04mm；The collagen prepared through a step/polycaprolactone solution is placed in
In the centrifuge tube of centrifugal spinning instrument, centrifuge tube revolving speed is 2000r/min, and cylinder mold main shaft is to spinning head distance
10cm, centrifugation spin nanometer film with a thickness of 0.1mm, and cylinder mold is removed to get to the complete artificial blood vessel prepared.
Claims (6)
1. a kind of method for reducing artificial blood vessel permeance property, which is characterized in that the preparation method sequentially includes the following steps:
A. collagen/polycaprolactone solution preparation
Collagen/polycaprolactone is dissolved in hexafluoroisopropanol, liquid quality fraction is 10%~20%, with 200~800r/
The speed of min carries out the stirring that the time is 10~18h under 20~60 DEG C of environment；The collagen of homogeneous transparent is obtained after dissolution completely
Albumen/polycaprolactone solution, by the collagen after the completion of stirring/polycaprolactone solution be put into vacuum environment (0.1~
Under 0.05MPa) 2~6h of deaeration to get arrive uniform collagen/polycaprolactone solution；
Wherein, the ratio of collagen and polycaprolactone is 3:7~7:3 in solution；
B. the preparation of artificial blood vessel
Tubing is weaved on woven machine, tubing is fixed on cylinder mold, cylinder mold is placed in volume
On machine, the up- coiler speed of mainshaft is 50~100r/min；The collagen for preparing in right amount through step a/gather oneself is dipped with brush
Lactone solution brushing makes tubing surface coat one layer of thin and uniform collagen/gather oneself on tubing outer surface
Lactone solution layer, with a thickness of 0.04~0.1mm；The collagen prepared through a step/polycaprolactone solution is placed in centrifugal spinning
In the centrifuge tube of instrument, centrifuge tube revolving speed be 2000~7000r/min, cylinder mold main shaft to spinning head distance be 10~
20cm, centrifugation spin nanometer film with a thickness of 0.1~0.5mm, and cylinder mold is removed to get to the complete artificial blood prepared
Pipe；
Wherein, cylinder mold is metal die or polyester or ceramics or Teflon mould.
2. a kind of method for reducing artificial blood vessel permeance property as described in claim 1, it is characterised in that: the tubulose is knitted
Yarn used in object is the blended material of one or in which multiple formation in terylene or spandex or silk or cotton.
3. a kind of method for reducing artificial blood vessel permeance property as described in claim 1, it is characterised in that: the tubulose is knitted
Object table inner layer basic organization is plain weave, twill-weave or satin weave.
4. a kind of method for reducing artificial blood vessel permeance property as described in claim 1, it is characterised in that: the tubulose is knitted
Object through it is close be 100~600/5cm, filling density is 100~600/5cm.
5. a kind of method for reducing artificial blood vessel permeance property as described in claim 1, it is characterised in that: the tubulose is knitted
Thickness of yarn used in object is 10~100D.
6. a kind of method for reducing artificial blood vessel permeance property as described in claim 1, it is characterised in that: the tubulose is knitted
Object diameter is 5~10mm.
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