CN109329939A - A kind of ganoderma lucidum functional food and preparation method thereof - Google Patents
A kind of ganoderma lucidum functional food and preparation method thereof Download PDFInfo
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- CN109329939A CN109329939A CN201811516387.0A CN201811516387A CN109329939A CN 109329939 A CN109329939 A CN 109329939A CN 201811516387 A CN201811516387 A CN 201811516387A CN 109329939 A CN109329939 A CN 109329939A
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- enzymatic hydrolysis
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 63
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 63
- 235000013376 functional food Nutrition 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 115
- 108090000790 Enzymes Proteins 0.000 claims abstract description 115
- 239000000203 mixture Substances 0.000 claims abstract description 88
- 238000009472 formulation Methods 0.000 claims abstract description 74
- 210000000582 semen Anatomy 0.000 claims abstract description 68
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 64
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 239000002994 raw material Substances 0.000 claims abstract description 41
- 241000371652 Curvularia clavata Species 0.000 claims abstract description 32
- 241000233866 Fungi Species 0.000 claims abstract description 30
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 24
- 241000218231 Moraceae Species 0.000 claims abstract description 23
- 230000009849 deactivation Effects 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 14
- 240000008866 Ziziphus nummularia Species 0.000 claims abstract 4
- 229940088598 enzyme Drugs 0.000 claims description 109
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 75
- 239000000284 extract Substances 0.000 claims description 44
- 230000000694 effects Effects 0.000 claims description 43
- 238000010828 elution Methods 0.000 claims description 39
- 239000003480 eluent Substances 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 108010059892 Cellulase Proteins 0.000 claims description 24
- 229940106157 cellulase Drugs 0.000 claims description 24
- 240000001549 Ipomoea eriocarpa Species 0.000 claims description 18
- 235000005146 Ipomoea eriocarpa Nutrition 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 239000004365 Protease Substances 0.000 claims description 16
- 238000004262 preparative liquid chromatography Methods 0.000 claims description 16
- 239000007864 aqueous solution Substances 0.000 claims description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 13
- 229940111205 diastase Drugs 0.000 claims description 13
- 102000012286 Chitinases Human genes 0.000 claims description 12
- 108010022172 Chitinases Proteins 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 108090000526 Papain Proteins 0.000 claims description 11
- 229940055729 papain Drugs 0.000 claims description 11
- 235000019834 papain Nutrition 0.000 claims description 11
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 239000003513 alkali Substances 0.000 claims description 10
- 240000007233 Ipomoea indica Species 0.000 claims description 9
- 239000001509 sodium citrate Substances 0.000 claims description 9
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 9
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 9
- 229960001763 zinc sulfate Drugs 0.000 claims description 9
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 8
- 229930003268 Vitamin C Natural products 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- 235000019154 vitamin C Nutrition 0.000 claims description 8
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 7
- 241000207890 Ipomoea purpurea Species 0.000 claims description 6
- 238000004587 chromatography analysis Methods 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 229960001790 sodium citrate Drugs 0.000 claims description 6
- 235000019419 proteases Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 229960003390 magnesium sulfate Drugs 0.000 claims description 4
- 229960002713 calcium chloride Drugs 0.000 claims description 3
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 229920001221 xylan Polymers 0.000 claims description 2
- 150000004823 xylans Chemical class 0.000 claims description 2
- 244000189799 Asimina triloba Species 0.000 claims 1
- 235000006264 Asimina triloba Nutrition 0.000 claims 1
- 235000009467 Carica papaya Nutrition 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 240000000249 Morus alba Species 0.000 claims 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims 1
- 239000005864 Sulphur Substances 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 235000019640 taste Nutrition 0.000 abstract description 7
- 239000003963 antioxidant agent Substances 0.000 abstract description 3
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- 235000006708 antioxidants Nutrition 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
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- 239000000470 constituent Substances 0.000 abstract description 2
- 241001247821 Ziziphus Species 0.000 description 20
- 239000000047 product Substances 0.000 description 19
- 239000000945 filler Substances 0.000 description 17
- 238000010792 warming Methods 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 11
- 239000008103 glucose Substances 0.000 description 11
- 239000004615 ingredient Substances 0.000 description 10
- 230000001225 therapeutic effect Effects 0.000 description 9
- 229920001282 polysaccharide Polymers 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 150000004804 polysaccharides Chemical class 0.000 description 7
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 6
- 229920001542 oligosaccharide Polymers 0.000 description 6
- 150000002482 oligosaccharides Chemical class 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 150000002772 monosaccharides Chemical class 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 238000013329 compounding Methods 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000035922 thirst Effects 0.000 description 3
- 206010010774 Constipation Diseases 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 210000003764 chromatophore Anatomy 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000118 hair dye Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 206010049865 Achromotrichia acquired Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 208000025309 Hair disease Diseases 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000872198 Serjania polyphylla Species 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 235000003434 Sesamum indicum Nutrition 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- AEMOLEFTQBMNLQ-BKBMJHBISA-N alpha-D-galacturonic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-BKBMJHBISA-N 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 235000007215 black sesame Nutrition 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 208000034653 disorder of pilosebaceous unit Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000003779 hair growth Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000036651 mood Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/40—Fermented products; Products treated with microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
- A23L7/107—Addition or treatment with enzymes not combined with fermentation with microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of ganoderma lucidum functional foods and preparation method thereof, belong to food technology field.The ganoderma lucidum functional food includes the first component and the second component.First component carries out the first enzymatic hydrolysis to the first raw material for including ganoderma lucidum and black fungus by the first complex enzyme formulation and is made.Second component carries out the second enzymatic hydrolysis to the second raw material for including semen sojae atricolor, Semen sesami nigrum, black rice, jujube and mulberries by the second complex enzyme formulation and is made.Rationally, taste is preferable for functional food formula, has and promotes changing white hair into black hair, anti-oxidant and the healthcare functions such as improve the immunity of the human body, quick.Preparation method includes carrying out the first enzymatic hydrolysis to the first raw material using the first complex enzyme formulation, and subsequent enzyme deactivation obtains the first enzymolysis liquid；Second enzymatic hydrolysis is carried out to the second raw material using the second complex enzyme formulation, subsequent enzyme deactivation obtains the second enzymolysis liquid.Mix the first enzymolysis liquid and the second enzymolysis liquid.This method is simple, can largely retain the active constituent in material composition.
Description
Technical field
The present invention relates to food technology fields, and in particular to a kind of ganoderma lucidum functional food and preparation method thereof.
Background technique
Hair, which is not only human head, uniquely can freely modify, show the growth-gen and human health of self elegance
The marker of situation.As economic development and living standard increasingly improve, people are increasingly heavier to personal image and health
Depending on all thirsting for possessing a pitch-black, submissive, dense, rich glossiness healthy hair.But since working and learning pressure is big, lives not
The reasons such as rule, mood fluctuation, inherent cause and individual physique, many people early give birth to white hair, it is serious affect it is humanoid
As and daily life.What is more, body also will appear relevant diseases, influence Liver and kidney function and blood circulation, seriously endanger people
Body health.
Currently, the treatment of white hair disease there is no ideal method, existing treatment method is had the following deficiencies: (1) slow effect,
The course for the treatment of is long, and curative effect is not ideal enough；(2) raw material type is complicated, high production cost；(3) taste bad, consumer are difficult to receive；(5) it deposits
In safety problems such as toxic side effect, heavy metals exceeding standards.
In consideration of it, most people like being dyed the hair black with hair dye etc., but hair dye contains lead, mercury, azo mostly
The harmful substances such as object are closed, the risk of the malignant diseases such as being used for a long time, which will increase, suffers from breast cancer, lymph cancer, leukaemia.Therefore, seek
A kind of mode of effective and feasible changing white hair into black hair, has great importance.
Summary of the invention
The first object of the present invention is to provide a kind of ganoderma lucidum functional food, functional food formula rationally, taste
Preferably, have the function of significantly promoting changing white hair into black hair, and quick.
The second object of the present invention is to provide a kind of preparation method of above-mentioned functional food, and this method is simple, can
Largely retain the active constituent in material composition.
The present invention solves its technical problem and adopts the following technical solutions to realize:
The present invention proposes a kind of ganoderma lucidum functional food, which includes the first component and the second component.
First component is by the first complex enzyme formulation of 0.05-0.1 parts by weight to the ganoderma lucidum and 5- for including 5-25 parts by weight
First raw material of the black fungus of 15 parts by weight carries out the first enzymatic hydrolysis and is made.
Second component is by the second complex enzyme formulation of 0.05-0.1 parts by weight to semen sojae atricolor, 15- including 15-25 parts by weight
The Semen sesami nigrum of 25 parts by weight, the black rice of 5-15 parts by weight, the jujube of 5-15 parts by weight and 5-15 parts by weight mulberries it is second former
Material carries out the second enzymatic hydrolysis and is made.
Preferably, the second enzymatic hydrolysis is carried out in the presence of the modulator of 0.1-0.2 parts by weight.
Preferably, the first complex enzyme formulation includes cellulase, chitinase, papain and pectase.
Preferably, the second complex enzyme formulation includes cellulase, mesophilicα-diastase, zytase and basic protein
Enzyme.
Preferably, modulator includes calcium chloride, magnesium sulfate, zinc sulfate, sodium citrate and vitamin C.
Preferably, semen sojae atricolor is the semen sojae atricolor through frying；Semen sesami nigrum is the Semen sesami nigrum through frying；Black rice is the black rice through frying.
Further, the raw material of above-mentioned functional food further includes the flower extract of leading a cow of 8-12 parts by weight.
Lead a cow flower extract by morning glory after methanol aqueous solution extracts, collect supernatant, then in macroporous absorbent resin
Gradient elution in chromatographic column collects third elution fraction, then purifies and obtain through preparative liquid chromatography.
Preferably, morning glory is blue morning glory and/or purple morning glory.
Preferably, the condition of gradient elution includes: using methanol-water solution as eluent, by body of the methanol in eluent
The ratio that product percentage is followed successively by 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution, washes used in each gradient
The volume of de- liquid is 500-600mL；
Preferably, the condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial of first alcohol and water
Volume ratio is 10:90, and final volume ratio is 80:20 gradient elution 30-40min.
The present invention also proposes the preparation method of above-mentioned functional food, comprising the following steps:
First enzymatic hydrolysis is carried out to the first raw material using the first complex enzyme formulation, subsequent enzyme deactivation obtains the first enzymolysis liquid.
Second enzymatic hydrolysis is carried out to the second raw material using the second complex enzyme formulation, subsequent enzyme deactivation obtains the second enzymolysis liquid.
Mix the first enzymolysis liquid and the second enzymolysis liquid.
Preferably, the first enzymatic hydrolysis carries out 2-3h under conditions of 50-55 DEG C.
Preferably, the second enzymatic hydrolysis carries out 2-3h under conditions of 50-55 DEG C.
Preferably, the second enzymatic hydrolysis is carried out in the presence of the modulator of 0.1-0.2 parts by weight.
The beneficial effect for the ganoderma lucidum functional food and preparation method thereof that present pre-ferred embodiments provide includes:
Rationally, taste is preferable, has and significantly promotees for the ganoderma lucidum functional food formula that present pre-ferred embodiments provide
Into the effect of changing white hair into black hair, and it is quick.Preparation method is simple, can largely retain the activity in material composition
Ingredient.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Ganoderma lucidum functional food of the application and preparation method thereof is specifically described below.
Ganoderma lucidum functional food involved in the application comprising the first component and the second component.
Wherein, the first component is by the first complex enzyme formulation of 0.05-0.1 parts by weight to the ganoderma lucidum including 5-25 parts by weight
The first enzymatic hydrolysis is carried out with the first raw material of the black fungus of 5-15 parts by weight to be made.
Second component is by the second complex enzyme formulation of 0.05-0.1 parts by weight to semen sojae atricolor, 15- including 15-25 parts by weight
The Semen sesami nigrum of 25 parts by weight, the black rice of 5-15 parts by weight, the jujube of 5-15 parts by weight and 5-15 parts by weight mulberries it is second former
Material carries out the second enzymatic hydrolysis and is made.
Preferably, above-mentioned second enzymatic hydrolysis is carried out in the presence of the modulator of 0.1-0.2 parts by weight.
Optionally, in said components the parts by weight of ganoderma lucidum, semen sojae atricolor and Semen sesami nigrum can individually for 15,16,17,
18,19,20,21,22,23,24 or 25 parts by weight etc., can also individually for 15.5,16.5,17.5,18.5,19.5,20.5,
21.5,22.5,23.5 or 24.5 parts by weight etc. can be individually additionally any parts by weight numerical value within the scope of 15-25.
Black fungus, black rice, jujube and mulberries parts by weight can individually be 5,6,7,8,9,10,11,12,13,14
Or 15 parts by weight etc., it can also individually be 5.5,6.5,7.5,8.5,9.5,10.5,11.5,12.5,13.5 or 14.5 weight
Part etc., it can be individually additionally any parts by weight numerical value within the scope of 5-15.
The parts by weight of first complex enzyme formulation and the second complex enzyme formulation can individually for 0.05,0.06,0.07,
0.08, furthermore 0.09 or 0.1 parts by weight etc. can also be individually any parts by weight numerical value within the scope of 0.05-0.1.
Modulator can be then 0.1,0.15 or 0.2 parts by weight etc., or any parts by weight within the scope of 0.1-0.2
Value.
In some embodiments, the first component is by the first complex enzyme formulation of 0.06-0.09 parts by weight to including 18-
First raw material of the black fungus of the ganoderma lucidum and 8-12 parts by weight of 22 parts by weight carries out the first enzymatic hydrolysis and is made.Second component passes through
Black sesame of second complex enzyme formulation of 0.06-0.09 parts by weight to semen sojae atricolor, 17.5-22.5 parts by weight including 18-22 parts by weight
Second raw material of the mulberries of fiber crops, the black rice of 8-12 parts by weight, the jujube of 8-12 parts by weight and 7.5-12.5 parts by weight carries out second
Enzymatic hydrolysis is made.
Preferably, above-mentioned second enzymatic hydrolysis is carried out in the presence of the modulator of 0.14-0.16 parts by weight.
In a specific embodiment, the first component is by the first complex enzyme formulation of 0.075 parts by weight to including 20
First raw material of the black fungus of the ganoderma lucidum of parts by weight and 10 parts by weight carries out the first enzymatic hydrolysis and is made.Second component passes through 0.075 weight
Measure part the second complex enzyme formulation to include the semen sojae atricolor of 20 parts by weight, the Semen sesami nigrum of 20 parts by weight, 10 parts by weight black rice, 10 weights
The second enzymatic hydrolysis of the second raw material progress for measuring the jujube of part and the mulberries of 10 parts by weight is made.In the specific embodiment,
The second enzymatic hydrolysis is carried out in the presence of the modulator of 0.15 parts by weight.
Wherein, ganoderma lucidum and black fungus can be directly the ganoderma lucidum of artificial cultivation or the fructification of black fungus, be also possible to by
Fructification drying, dry or natural air drying after resulting nothing rot with mildew substance.Ganoderma lucidum and black fungus are in this application
Positive effect can be played to changing white hair into black hair, be improved the immunity of the human body in addition, ganoderma lucidum also has, it is clearing heat and detoxicating, protect painstaking effort
The effects of pipe and anti-aging；Black fungus also have nourishing, moisturize, blood-nourishing benefit stomach, promoting blood circulation and hemostasis, moistening lung and ease constipation etc. are made
With.
Semen sojae atricolor can play the role of in this application it is black dark hair and must promote longevity, additionally have moistening lung and clearing heat,
Activating blood and promoting diuresis, blood-enriching tranquillizing, improving eyesight invigorating the spleen, kidney tonifying benefiting yin and removing toxic substances and other effects.
Semen sesami nigrum can be used in increasing internal melanin in the application, be conducive to natural on-off cycles of hair growth, in addition, its also each have it is strong
Stomach, liver protection and the effect for promoting red blood cell growth.
Black rice in this application, is mainly used in appetizing benefit, invigorating the spleen promoting blood circulation, improving eyesight and anti-aging etc., and can supplement human body
The several mineral materials such as the protein, manganese and the zinc that need.
The effects of mulberries (dry) in this application, can play enriching yin of enriching blood, promote the production of body fluid to quench thirst, and ease constipation is dry, and head can be improved
It swoons dizzy, tinnitus palpitaition, irritated insomnia, soreness and weakness of waist and knees, poliosis, the diseases such as dry of quenching one's thirst and dry and hard excrement.
In this application, the enriching yin that is mainly used for enriching blood promotes the production of body fluid to quench thirst and growing and blacking hair etc. jujube.
In some embodiments, the moisture of above-mentioned ganoderma lucidum, black fungus, semen sojae atricolor, Semen sesami nigrum, black rice, jujube and mulberries contains
Amount can be respectively 5-12wt%, and correspondingly, dry matter content is then 88-95%.
In the application, the first complex enzyme formulation cellulase, chitinase, papain and pectase enzyme activity according to
Secondary is 20000-30000u/g, 5000-6000u/g, 20000-30000u/g and 5000-6000u/g；That is, every the first complex enzyme of g
Preparation cellulase, chitinase, papain and pectase enzyme activity be followed successively by 20000-30000u, 5000-
6000u, 20000-30000u and 5000-6000u.
Second complex enzyme formulation cellulase, mesophilicα-diastase, zytase and alkali protease enzyme activity successively
For 20000-30000u/g, 2000-3000u/g, 5000-6000u/g and 50000-60000u/g；That is, every the second complex enzyme system of g
Agent cellulase, mesophilicα-diastase, zytase and alkali protease enzyme activity be followed successively by 20000-30000u, 2000-
3000u, 5000-6000u and 50000-60000u.Wherein, mesophilicα-diastase is relatively stable at 60 DEG C or less, most suitable action temperature
Degree is 60-70 DEG C.
Various enzymes in above-mentioned first complex enzyme formulation and the second complex enzyme formulation are purchased from Tai'an letter and get profit biology
Science and Technology Ltd..
In this application, the cellulase in the first complex enzyme formulation is mainly used for the cellulose in ganoderma lucidum and black fungus
It is hydrolyzed to the ingredients such as polysaccharide, oligosaccharides and glucose sugar；Chitinase is mainly used for for the chitin in ganoderma lucidum and black fungus being hydrolyzed to
The ingredients such as polysaccharide, oligosaccharides and monosaccharide；Pectase is mainly used for the pectic substance in black fungus being hydrolyzed to polysaccharide, oligosaccharides and monosaccharide etc.
Ingredient；Papain is mainly used for the protein in ganoderma lucidum and black fungus being hydrolyzed to the ingredients such as peptide and amino acid.
Cellulase in second complex enzyme formulation is mainly used for the fibre in semen sojae atricolor, Semen sesami nigrum, black rice, jujube and mulberries
Dimension element is hydrolyzed to the ingredients such as polysaccharide, oligosaccharides and glucose；Mesophilicα-diastase is mainly used for Semen sojae atricolor, Semen sesami nigrum, black rice, big
Starch Hydrolysis in jujube and mulberries is the ingredients such as dextrin, oligosaccharides and monosaccharide；Zytase be mainly used for by Semen sojae atricolor, Semen sesami nigrum,
Xylan hydrolysis in black rice, jujube and mulberries is the ingredients such as polysaccharide, oligosaccharides and monosaccharide；Alkali protease is mainly used for semen sojae atricolor
Protein in son, Semen sesami nigrum, black rice, jujube and mulberries is hydrolyzed to the ingredients such as peptide and amino acid.
In other embodiments, the glucose as filler can be also mixed in the first complex enzyme formulation, similarly,
The glucose as filler can also be mixed in second complex enzyme formulation.
In some embodiments, in modulator between calcium chloride, magnesium sulfate, zinc sulfate, sodium citrate and vitamin C
Weight ratio such as can be 18-22:4-6:4-6:35-45:25-35.It is preferably carried out in mode some, chlorine in modulator
Changing the weight ratio between calcium, magnesium sulfate, zinc sulfate, sodium citrate and vitamin C is 20:5:5:40:30.By above-mentioned preferred
Matching resulting adjusting material can play the role of good anti-oxidant in the second enzymolysis process and stablize enzyme activity.
From the above, the application is using the black cereals such as edible and medicinal fungis and semen sojae atricolor, Semen sesami nigrum such as ganoderma lucidum and black fungus connection
The side of being combined, formula is scientific and reasonable, promotes the effect of changing white hair into black hair significant, with the ratio of similar product on the market, has quick, treatment
Imitate the advantages such as lasting, safe and non-toxic, features good taste, convenient.
Ganoderma lucidum and black fungus are digested using the first complex enzyme formulation, by the macromolecular polysaccharides substance such as its cellulose
Micromolecular polysaccharide is resolved into, molecular weight reduces, and absorption rate improves, and bioactivity is stronger, meanwhile, physicochemical characteristic and taste
It is improved, the bitter taste of especially ganoderma lucidum substantially reduces, and palatability is stronger.
The raw materials such as semen sojae atricolor, Semen sesami nigrum grade black rice are digested using the second complex enzyme formulation, protein is hydrolyzed to peptide,
With lower molecular weight and good physicochemical characteristic, it is easier to by human consumption and absorption, but also there is anti-oxidant, drop blood
Pressure reduces blood lipid and cholesterol, mitigates sports fatigue, promotes fat and energetic supersession, improving sleep, adjusting immune system and mention
The effect of high body immunity.
In some more preferably embodiments, the ganoderma lucidum functional food of the application further includes leading for 8-12 parts by weight
Ox flower extract.Optionally, flower extract of leading a cow can be 8,9,10,11 or 12 parts by weight, or 8.5,9.5,
10.5 or 11.5 parts by weight can also be any parts by weight numerical value within the scope of 8-12.
For the flower extract of leading a cow by morning glory after methanol aqueous solution extracts, filtering will be set forth in macroporous absorption tree on filtrate
Gradient elution is carried out in rouge chromatographic column, collects third elution fraction, then purifies and obtains through preparative liquid chromatography, collects content most
High chromatographic peak, flower extract of as leading a cow mentioned by the application.
Specifically, fresh morning glory is mixed in the ratio that solid-liquid ratio is 1g:200mL-300mL with methanol aqueous solution,
48-52h is extracted in stirring under conditions of 30-35 DEG C and 500-600r/min.Wherein, in methanol aqueous solution methanol volume hundred
Score is 30-50%.
The internal diameter of macroporous adsorption resin chromatography column is 4.5cm, and filler uses HP-20, and height of the filler in chromatographic column is
50-55cm。
By the extracting solution filtering after methanol aqueous solution extracts, concentration obtains medicinal extract.1mg is taken to be dissolved in 5-6mL water, so
Loading afterwards then carries out gradient elution with eluent.Remaining medicinal extract is carried out by identical column operation of crossing.
Preferably, the condition of gradient elution includes: using methanol-water solution as eluent, by body of the methanol in eluent
The ratio that product percentage is followed successively by 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution (total six elutions ladder
Degree), the volume of eluent used in each gradient is 500-600mL.
Collect wherein third elution fraction, namely the institute after methanol volumetric concentration is the methanol-water solution elution of 40vt%
The component obtained is purified after dry with preparative liquid chromatography.
Preferably, the condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial of first alcohol and water
Volume ratio is 10:90, and final volume ratio is 80:20 gradient elution 30-40min, and the elution program is directly by preparative liquid
It is arranged in phase chromatography.Elution flow rate can be 18-20mL/min.The highest chromatographic peak of content is collected, it is dry.
It is worth noting that study for a long period of time discovery through inventor, by by the above-mentioned flower extract and above-mentioned ganoderma lucidum etc. of leading a cow
Raw material cooperation can cooperate with the effect for playing apparent prevention and treatment white hair, also, morning glory can only be blue morning glory and/or purple
Color morning glory, if change into other colors such as bright red or pink morning glory its do not have synergistic function substantially.
The mechanism of action of the extract of blue morning glory and/or purple morning glory may include following aspect: first, above-mentioned
Flower extract of leading a cow can increase the activity of tyrosinase in body, to promote the generation of melanin；Second, above-mentioned lead a cow
Flower extract can activate the gene having an impact to chromatophore, enhance the proliferative capacity of chromatophore, secret out of it more
More melanin.
It in addition, present invention also provides a kind of preparation methods of above-mentioned ganoderma lucidum functional food, such as may include following
Step: the first enzymatic hydrolysis is carried out to the first raw material using the first complex enzyme formulation, subsequent enzyme deactivation obtains the first enzymolysis liquid.Using
Two complex enzyme formulations carry out the second enzymatic hydrolysis to the second raw material, and subsequent enzyme deactivation obtains the second enzymolysis liquid.Mix the first enzymolysis liquid and
Two enzymolysis liquids.
It preferably, can first frying semen sojae atricolor, Semen sesami nigrum and black rice before enzymatic hydrolysis.By to semen sojae atricolor, Semen sesami nigrum and black rice
Equal raw materials use high temperature frying, can produce strong fragrance, keep the palatability of product more preferable.
Preferably, before the first enzymatic hydrolysis, ganoderma lucidum and black fungus are crushed to 100-120 mesh, are then 1:8- by solid-to-liquid ratio
10 mix with water, are tuned into pulpous state, then mix with the first complex enzyme formulation, digest 2-3h for the first time under conditions of 50-55 DEG C.
After first enzymatic hydrolysis, it is warming up to 90-95 DEG C, maintains 8-10min, then enzyme deactivation is rapidly cooled to 50-60 DEG C, obtains first
Enzymolysis liquid.
Similarly, before the second enzymatic hydrolysis, after the semen sojae atricolor after jujube, mulberries, frying, the Semen sesami nigrum after frying and frying
Black rice is crushed to 100-120 mesh, is then mixed with water by solid-to-liquid ratio 1:8-10, is tuned into pulpous state, then with the second complex enzyme formulation
And modulator mixing, second of enzymatic hydrolysis 2-3h under conditions of 50-55 DEG C.After second enzyme solution, it is warming up to 90-95 DEG C,
8-10min is maintained, then enzyme deactivation is rapidly cooled to 50-60 DEG C, obtains the second enzymolysis liquid.
Further, further includes: mixed mixed enzymolysis liquid is concentrated in vacuo and is spray-dried.Wherein, vacuum
The vacuum degree of concentration is 0.06-0.08MPa, and temperature is 70-80 DEG C；Being concentrated in vacuo to solid content is 25-30wt%.It is spraying
Drying carries out under conditions of inlet temperature is 180-200 DEG C, outlet temperature is 70-80 DEG C.
It is worth noting that when in raw material also containing lead a cow flower extract when, will mixed enzymolysis liquid it is dry after obtain it is mixed
Zymolyte is closed to mix with the flower extract of leading a cow after drying.
The eating method of the ganoderma lucidum functional food can be with are as follows: mixes the product with appropriate warm water, stirs into atherosclerotic, directly
It connects edible；Daily recommendation amount is 40-60 grams.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
Ganoderma lucidum functional food provided in this embodiment includes the first component and the second component, and the first component passes through 0.075
First complex enzyme formulation of parts by weight carries out the to the first raw material of the ganoderma lucidum for including 20 parts by weight and the black fungus of 10 parts by weight
One enzymatic hydrolysis is made.Second component is by the second complex enzyme formulation of 0.075 parts by weight to semen sojae atricolor, 20 weight for including 20 parts by weight
Part Semen sesami nigrum, the black rice of 10 parts by weight, the jujube of 10 parts by weight and 10 parts by weight the second raw material of mulberries carry out second enzyme
Solution is made, and the second enzymatic hydrolysis is existing for the modulator of 0.15 parts by weight under the conditions of carries out.
Above-mentioned first complex enzyme formulation is limited by the glucose as filler and purchased from Tai'an letter biotechnology of getting profit
Cellulase, chitinase, papain and the pectase of company are 68.17:8.33:5.5:5.5 according to weight ratio:
12.5 compounding.The enzyme activity of resulting first complex enzyme formulation cellulase, chitinase, papain and pectase
It is followed successively by 25000u/g, 5500u/g, 25000u/g and 5500u/g.
Second complex enzyme formulation is got profit Biotechnology Co., Ltd by the glucose as filler and purchased from Tai'an letter
Cellulase, mesophilicα-diastase, zytase and alkali protease be by weight 33.67:8.33:25:5.5:
27.5 compounding.Resulting second complex enzyme formulation cellulase, mesophilicα-diastase, zytase and alkali protease
Enzyme activity be followed successively by 25000u/g, 2500u/g, 5500u/g and 55000u/g.
Calcium chloride, magnesium sulfate, zinc sulfate, sodium citrate and the vitamin that modulator is 20:5:5:40:30 by weight ratio
C composition.
The preparation method of above-mentioned ganoderma lucidum functional food includes:
(1) frying of Semen sojae atricolor, Semen sesami nigrum and black rice
Semen sojae atricolor is cleaned, draining, is air-dried, is poured into rotary frying pan, middle small fire frying, and stalling is not dynamic, is fried ripe to beans
Thoroughly, beans fragrance spread out, stops frying, cools completely spare；
Semen sesami nigrum is cleaned, draining, air-dries, pours into rotary frying pan, middle small fire frying, and stalling is not dynamic, fries and arrives Semen sesami nigrum
Well-done, sesame fragrance spread out, stops frying, cools completely spare；
Black rice is cleaned, draining, is air-dried, is poured into rotary frying pan, middle small fire frying, and stalling is not dynamic, and stir-fry is well-done to the grain of rice,
Rice fragrance spread out, stops frying, cools completely spare；
(2) it digests
The ganoderma lucidum of prescription amount and black fungus are mixed, 110 mesh are crushed to.Then, pure water is added by solid-to-liquid ratio 1:9, adjusts
It is slurried, natural ph.The first complex enzyme formulation of prescription amount is added, is uniformly mixed.52.5 DEG C are warming up to, first time enzyme
Solve 2.5h.After first enzymatic hydrolysis, 92.5 DEG C are warming up to, maintains 9min, enzyme deactivation.Then 55 DEG C are rapidly cooled to, obtains first
Enzymolysis liquid.
The semen sojae atricolor of prescription amount, Semen sesami nigrum, black rice, jujube and mulberries are dry-mixed even, it is crushed to 110 mesh.Then, by solid-to-liquid ratio
Pure water is added in 1:9, modulates pulp, natural ph.The second complex enzyme formulation and adjusting material of prescription amount are added, mixing is equal
It is even.52.5 DEG C are warming up to, second enzymatic hydrolysis 2.5 hours.After second enzyme solution, 92.5 DEG C are warming up to, maintains 9min, enzyme deactivation.
Then 55 DEG C are rapidly cooled to, the second enzymolysis liquid is obtained.
The first enzymolysis liquid and the second enzymolysis liquid are mixed, it is spare.
(3) it is concentrated in vacuo
By mixed enzymolysis liquid in vacuum degree be 0.07MPa, temperature be 75 DEG C under conditions of be concentrated in vacuo to solid content
It is 28%.
(4) it is spray-dried
Concentrate resulting after vacuum concentration is carried out under conditions of inlet temperature is 190 DEG C, outlet temperature is 75 DEG C
Spray drying, gained dry powder is the ganoderma lucidum functional food of the present embodiment.
Embodiment 2
The present embodiment the difference from embodiment 1 is that: the first component passes through the first complex enzyme formulation pair of 0.05 parts by weight
First raw material of the black fungus of ganoderma lucidum and 15 parts by weight including 15 parts by weight carries out the first enzymatic hydrolysis and is made.Second component passes through
Second complex enzyme formulation of 0.05 parts by weight to include the semen sojae atricolor of 15 parts by weight, the Semen sesami nigrum of 25 parts by weight, 5 parts by weight it is black
Second raw material of the mulberries of rice, the jujube of 15 parts by weight and 5 parts by weight carries out the second enzymatic hydrolysis and is made, and the second enzymatic hydrolysis is 0.1
It is carried out under the conditions of the modulator of parts by weight is existing.
Embodiment 3
The present embodiment the difference from embodiment 1 is that: the first component is by the first complex enzyme formulation of 0.1 parts by weight to packet
The first enzymatic hydrolysis of the first raw material progress for including the ganoderma lucidum of 25 parts by weight and the black fungus of 5 parts by weight is made.Second component passes through 0.1 weight
Measure part the second complex enzyme formulation to include the semen sojae atricolor of 25 parts by weight, the Semen sesami nigrum of 15 parts by weight, 15 parts by weight black rice, 5 weights
The second enzymatic hydrolysis of the second raw material progress for measuring the jujube of part and the mulberries of 15 parts by weight is made, and the second enzymatic hydrolysis is in 0.2 parts by weight
Modulator it is existing under the conditions of carry out.
Embodiment 4
The present embodiment the difference from embodiment 1 is that: the first component passes through the first complex enzyme formulation pair of 0.06 parts by weight
First raw material of the black fungus of ganoderma lucidum and 8 parts by weight including 18 parts by weight carries out the first enzymatic hydrolysis and is made.Second component passes through
Second complex enzyme formulation of 0.06 parts by weight to include the semen sojae atricolor of 18 parts by weight, the Semen sesami nigrum of 17.5 parts by weight, 8 parts by weight it is black
Rice, the jujube of 8 parts by weight and 7.5 parts by weight the second raw material of mulberries carry out the second enzymatic hydrolysis and be made, and the second enzymatic hydrolysis be
It is carried out under the conditions of the modulator of 0.14 parts by weight is existing.
Embodiment 5
The present embodiment the difference from embodiment 1 is that: the first component passes through the first complex enzyme formulation pair of 0.09 parts by weight
First raw material of the black fungus of ganoderma lucidum and 12 parts by weight including 22 parts by weight carries out the first enzymatic hydrolysis and is made.Second component passes through
Second complex enzyme formulation of 0.09 parts by weight is to including the semen sojae atricolors of 22 parts by weight, the Semen sesami nigrum of 22.5 parts by weight, 12 parts by weight
Second raw material of the mulberries of black rice, the jujube of 12 parts by weight and 12.5 parts by weight carries out the second enzymatic hydrolysis and is made, and the second enzymatic hydrolysis is
It is carried out under the conditions of existing for the modulator of 0.16 parts by weight.
Embodiment 6
The present embodiment the difference from embodiment 1 is that: be free of in the first complex enzyme formulation and the second complex enzyme formulation
Glucose as filler.
Embodiment 7
The present embodiment the difference from embodiment 1 is that: the first complex enzyme formulation by as filler glucose and purchase
From Tai'an letter get profit Biotechnology Co., Ltd cellulase, chitinase, papain and pectase according to weight
Than being compounded for 63:10:6:6:15.First complex enzyme formulation cellulase, chitinase, papain and pectase
Enzyme activity be followed successively by 30000u/g, 6000u/g, 30000u/g and 6000u/g.
Second complex enzyme formulation is got profit Biotechnology Co., Ltd by the glucose as filler and purchased from Tai'an letter
Cellulase, mesophilicα-diastase, zytase and alkali protease be by weight 24:10:30:6:30 compounding and
At.Second complex enzyme formulation cellulase, mesophilicα-diastase, zytase and alkali protease enzyme activity be followed successively by
30000u/g, 3000u/g, 6000u/g and 60000u/g.
Embodiment 8
The present embodiment the difference from embodiment 1 is that: the first complex enzyme formulation by as filler glucose and purchase
From Tai'an letter get profit Biotechnology Co., Ltd cellulase, chitinase, papain and pectase according to weight
Than being compounded for 73.33:6.67:5:5:10.First complex enzyme formulation cellulase, chitinase, papain and
The enzyme activity of pectase is followed successively by 20000u/g, 5000u/g, 20000u/g and 5000u/g.
Second complex enzyme formulation is got profit Biotechnology Co., Ltd by the glucose as filler and purchased from Tai'an letter
Cellulase, mesophilicα-diastase, zytase and alkali protease by weight for 43.33:6.67:20:5:25 it is multiple
With forming.Second complex enzyme formulation cellulase, mesophilicα-diastase, zytase and alkali protease enzyme activity successively
For 20000u/g, 2000u/g, 5000u/g and 50000u/g.
Embodiment 9
The present embodiment the difference from embodiment 1 is that: modulator by weight ratio be 18:4:4:35:25 calcium chloride, sulfuric acid
Magnesium, zinc sulfate, sodium citrate and vitamin C composition.
Embodiment 10
The present embodiment the difference from embodiment 1 is that: modulator by weight ratio be 22:6:6:45:35 calcium chloride, sulfuric acid
Magnesium, zinc sulfate, sodium citrate and vitamin C composition.
Embodiment 11
The present embodiment the difference from embodiment 1 is that: carried out under the conditions of the second enzymatic hydrolysis is not existing for the modulator.
Embodiment 12
The present embodiment the difference from embodiment 1 is that: (2) to (4) in the preparation method of ganoderma lucidum functional food
Step carries out in the following way respectively:
(2) it digests
The ganoderma lucidum of prescription amount and black fungus are mixed, 110 mesh are crushed to.Then, pure water is added by solid-to-liquid ratio 1:8, adjusts
It is slurried, natural ph.The first complex enzyme formulation of prescription amount is added, is uniformly mixed.50 DEG C are warming up to, is digested for the first time
2h.After first enzymatic hydrolysis, 90 DEG C are warming up to, maintains 10min, enzyme deactivation.Then 50 DEG C are rapidly cooled to, the first enzymatic hydrolysis is obtained
Liquid.
The semen sojae atricolor of prescription amount, Semen sesami nigrum, black rice, jujube and mulberries are dry-mixed even, it is crushed to 100 mesh.Then, by solid-to-liquid ratio
Pure water is added in 1:8, modulates pulp, natural ph.The second complex enzyme formulation and adjusting material of prescription amount are added, mixing is equal
It is even.50 DEG C are warming up to, second enzymatic hydrolysis 2 hours.After second enzyme solution, 90 DEG C are warming up to, maintains 10min, enzyme deactivation.Then fast
Speed is cooled to 50 DEG C, obtains the second enzymolysis liquid.
The first enzymolysis liquid and the second enzymolysis liquid are mixed, it is spare.
(3) it is concentrated in vacuo
By mixed enzymolysis liquid in vacuum degree be 0.06MPa, temperature be 70 DEG C under conditions of be concentrated in vacuo to solid content
It is 25%.
(4) it is spray-dried
Concentrate resulting after vacuum concentration is carried out under conditions of inlet temperature is 180 DEG C, outlet temperature is 70 DEG C
Spray drying, gained dry powder is the ganoderma lucidum functional food of the present embodiment.
Embodiment 13
The present embodiment the difference from embodiment 1 is that: (2) to (4) in the preparation method of ganoderma lucidum functional food
Step carries out in the following way respectively:
(2) it digests
The ganoderma lucidum of prescription amount and black fungus are mixed, 120 mesh are crushed to.Then, pure water is added by solid-to-liquid ratio 1:10, adjusts
It is slurried, natural ph.The first complex enzyme formulation of prescription amount is added, is uniformly mixed.55 DEG C are warming up to, is digested for the first time
3h.After first enzymatic hydrolysis, 95 DEG C are warming up to, maintains 8min, enzyme deactivation.Then 60 DEG C are rapidly cooled to, the first enzymolysis liquid is obtained.
The semen sojae atricolor of prescription amount, Semen sesami nigrum, black rice, jujube and mulberries are dry-mixed even, it is crushed to 120 mesh.Then, by solid-to-liquid ratio
Pure water is added in 1:10, modulates pulp, natural ph.The second complex enzyme formulation and adjusting material of prescription amount are added, is mixed
Uniformly.55 DEG C are warming up to, second enzymatic hydrolysis 3 hours.After second enzyme solution, 95 DEG C are warming up to, maintains 8min, enzyme deactivation.Then
60 DEG C are rapidly cooled to, the second enzymolysis liquid is obtained.
The first enzymolysis liquid and the second enzymolysis liquid are mixed, it is spare.
(3) it is concentrated in vacuo
By mixed enzymolysis liquid in vacuum degree be 0.08MPa, temperature be 80 DEG C under conditions of be concentrated in vacuo to solid content
It is 30%.
(4) it is spray-dried
Concentrate resulting after vacuum concentration is carried out under conditions of inlet temperature is 200 DEG C, outlet temperature is 80 DEG C
Spray drying, gained dry powder is the ganoderma lucidum functional food of the present embodiment.
Embodiment 14
The present embodiment the difference from embodiment 1 is that: morning glory of the ganoderma lucidum functional food also containing 10 parts by weight is extracted
Object, the mixed enzymolysis object that ganoderma lucidum functional food obtains after mixed enzymolysis liquid is dry are mixed with the flower extract of leading a cow after drying
And it obtains.
The flower extract of leading a cow obtains in the following manner: the ratio for being 1g:250mL by solid-liquid ratio by fresh blue morning glory
Example is mixed with methanol aqueous solution, and 50h is extracted in stirring under conditions of 30 DEG C and 600r/min.Wherein, first in methanol aqueous solution
The percentage by volume of alcohol is 40%.By the extracting solution filtering after methanol aqueous solution extracts, concentration obtains medicinal extract.1mg is taken to be dissolved in
In 6mL water, then loading, then carries out gradient elution with eluent.Remaining medicinal extract is carried out by identical column operation of crossing.Cross column institute
The internal diameter of macroporous adsorption resin chromatography column is 4.5cm, and filler uses HP-20, and height of the filler in chromatographic column is 55cm.
The condition of gradient elution includes: using methanol-water solution as eluent, by percentage by volume of the methanol in eluent
The ratio for being followed successively by 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution (amounting to six gradients), each
The volume of eluent used in gradient is 550mL.Third elution fraction is collected, is purified after dry with preparative liquid chromatography.
The condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial volume ratio of first alcohol and water
For 10:90, final volume ratio is 80:20 gradient elution 35min, and the elution program in preparative liquid chromatography directly by setting
It sets.Elution flow rate is 20mL/min.The highest chromatographic peak of content is collected, it is dry.
Embodiment 15
The present embodiment and the difference of embodiment 14 are: ganoderma lucidum functional food contains the flower extract of leading a cow of 8 parts by weight.
Embodiment 16
The present embodiment and the difference of embodiment 14 are: the morning glory that ganoderma lucidum functional food contains 12 parts by weight is extracted
Object.
Embodiment 17
The present embodiment and the difference of embodiment 14 are: morning glory is purple morning glory.
Embodiment 18
The present embodiment and the difference of embodiment 14 are: morning glory is that blue morning glory is by weight with purple morning glory
1:1 is obtained by mixing.
Embodiment 19
The present embodiment and the difference of embodiment 14 are: flower extract of leading a cow obtains in the following manner: by fresh blue
Morning glory is mixed in the ratio that solid-liquid ratio is 1g:200mL with methanol aqueous solution, is stirred under conditions of 32.5 DEG C and 550r/min
It mixes and extracts 48h.Wherein, the percentage by volume of methanol is 30% in methanol aqueous solution.By the extraction after methanol aqueous solution extracts
Liquid filtering, concentration obtain medicinal extract.It takes 1mg to be dissolved in 5.5mL water, then loading, then carries out gradient elution with eluent.It is surplus
Remaining medicinal extract is carried out by identical column operation of crossing.The internal diameter for crossing macroporous adsorption resin chromatography column used in column is 4.5cm, and filler uses
HP-20, height of the filler in chromatographic column are 52.5cm.
The condition of gradient elution includes: using methanol-water solution as eluent, by percentage by volume of the methanol in eluent
The ratio for being followed successively by 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution (amounting to six gradients), each
The volume of eluent used in gradient is 500mL.Third elution fraction is collected, is purified after dry with preparative liquid chromatography.
The condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial volume ratio of first alcohol and water
For 10:90, final volume ratio is 80:20 gradient elution 30min, and the elution program in preparative liquid chromatography directly by setting
It sets.Elution flow rate is 19mL/min.The highest chromatographic peak of content is collected, it is dry.
Embodiment 20
The present embodiment and the difference of embodiment 14 are: flower extract of leading a cow obtains in the following manner: by fresh blue
Morning glory is mixed in the ratio that solid-liquid ratio is 1g:300mL with methanol aqueous solution, is stirred under conditions of 35 DEG C and 500r/min
Extract 52h.Wherein, the percentage by volume of methanol is 50% in methanol aqueous solution.By the extracting solution after methanol aqueous solution extracts
Filtering, concentration, obtains medicinal extract.It takes 1mg to be dissolved in 5mL water, then loading, then carries out gradient elution with eluent.Residue leaching
Cream is carried out by identical column operation of crossing.The internal diameter for crossing macroporous adsorption resin chromatography column used in column is 4.5cm, and filler uses HP-
20, height of the filler in chromatographic column is 50cm.
The condition of gradient elution includes: using methanol-water solution as eluent, by percentage by volume of the methanol in eluent
The ratio for being followed successively by 0%, 20%, 40%, 60%, 80% and 100% carries out gradient elution (amounting to six gradients), each
The volume of eluent used in gradient is 600mL.Third elution fraction is collected, is purified after dry with preparative liquid chromatography.
The condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial volume ratio of first alcohol and water
For 10:90, final volume ratio is 80:20 gradient elution 40min, and the elution program in preparative liquid chromatography directly by setting
It sets.Elution flow rate is 18mL/min.The highest chromatographic peak of content is collected, it is dry.
Test example 1
By taking the resulting ganoderma lucidum functional food of embodiment 1 as an example, following tests is carried out.This group test totally 64, is logical
The volunteer of network platform recruitment is crossed, it is 36 male, female 28, age the smallest volunteer 20 years old, maximum 58 years old, average age
36.2 years old.The course of disease is different in size, the longest 30 years, most short person 1 year, and average course of disease 12 years.5% or less person of white hair ratio 12,
5% or more, 20% or less person 20,20% or more person 32.
Treatment method: 64 volunteers are randomly divided into 2 groups in white hair ratio, every group of 32 people, i.e. every group of white hair ratio 5% with
Lower person 6,5% or more, 20% or less person 10,20% or more person 16.Control group: daily head massage 30 minutes；Treatment
Group: daily 50 grams of functional food of the invention of all cases, and engagement head is massaged 30 minutes, is monthly a treatment
Journey continuously treats 3 courses for the treatment of.White hair all switchs to black originator, to cure；Have in white hair 50% to become up to 100% or less
Black originator is significant effective；Have 20% in white hair to become black originator up to 50% or less, is effective；Have in white hair 20% with
Under become black originator, be invalid.
Treatment results: (1) it cures: control group totally 0, cure rate 0%；Treatment group totally 12, cure rate 37.5%.(2)
Significant effective: control group totally 0, significant effective percentage is 0%；Treatment group totally 18, significant effective percentage are 56.25%.(3) have
Effect: control group totally 0, effective percentage is 0%；Totally 2, effective percentage is 6.25%；(4) invalid: control group totally 32, inefficiency
100%；Treatment group totally 0, inefficiency 0%.
Wherein, Mr. Wang, male, 42 years old, certain houses market extension worker.It because operating pressure is big, goes on business throughout the year, life is not advised
Rule, local hair bleach, daily 50 grams of functional food of the invention, and engagement head is massaged 30 minutes, treat 1 month
White hair blackening afterwards, after treatment 3 months, white hair all becomes dark hair.
Zhang, male, 56 years old, university teacher.It because operating pressure is big, stays up late, has not enough sleep throughout the year, early raw white hair, in hair
White hair ratio be up to 30-40%, daily 50 grams of functional food of the invention, and engagement head is massaged 30 minutes,
Hair starts black by leucismus after treatment 1 month, and after treatment 3 months, white hair all becomes dark hair.
Zhang, female, 37 years old, private industry and commerce master.It is nervous because operating pressure is big, it lives irregular, often stays up late,
There is white hair in head.Before treatment, white hair accounting about 60-70%, daily 50 grams of functional food of the invention, and cooperating head
Portion is massaged 30 minutes, and hair starts black by leucismus after treatment 1 month, and after treatment 3 months, white hair all becomes dark hair.
Test example 2
According to the method for test example 1, to embodiment 2-4, embodiment 12 and the resulting ganoderma lucidum functional food of embodiment 13
Effect assessment is carried out, the results are shown in Table 1.
1 therapeutic effect of table (example)
| Embodiment 2 | Embodiment 3 | Embodiment 3 | Embodiment 4 | Embodiment 12 | Embodiment 13 | |
| It cures | 6 | 7 | 9 | 9 | 8 | 9 |
| It is significant effective | 13 | 13 | 14 | 15 | 15 | 16 |
| Effectively | 13 | 12 | 9 | 8 | 9 | 7 |
| In vain | 0 | 0 | 0 | 0 | 0 | 0 |
With cure and significant effective number of cases from the point of view of, table 1 is shown: in embodiment 1-5,1 resulting product of embodiment compared with
Embodiment 2-4 resulting product has more preferably therapeutic effect, and the therapeutic effect of embodiment 2-3 corresponding product is compared with embodiment 4-
5 is worse, illustrates to be more conducive to obtain the significantly more product of curative effect by the proportion ingredient of raw material each in embodiment 1.
In embodiment 1, embodiment 12 and embodiment 13,1 resulting product of embodiment is compared with embodiment 12 and embodiment 13
Resulting product has more preferably therapeutic effect, illustrates to be more conducive to obtain curative effect more by the preparation condition in embodiment 1
Significant product.
Test example 3
By taking embodiment 14 as an example, control group 1 is set and control group 2, control group 1 are with the difference of embodiment 14: being compareed
The flower extract of leading a cow of 10 parts by weight is replaced by the functional food powder of embodiment 1 in the ganoderma lucidum functional food of group 1, control
Group 2 is only the morning glory extract powder of 10 parts by weight.
It is functional to embodiment 1, embodiment 14, control group 1 and the resulting ganoderma lucidum of control group 2 according to the method for test example 1
Food carries out effect assessment, and the results are shown in Table 2.
2 therapeutic effect of table (example)
| Embodiment 1 | Embodiment 14 | Control group 1 | Control group 2 | |
| It cures | 12 | 20 | 13 | 3 |
| It is significant effective | 18 | 12 | 17 | 1 |
| Effectively | 2 | 0 | 2 | 26 |
| In vain | 0 | 0 | 0 | 2 |
To cure with from the point of view of significant effective number of cases, the therapeutic effect corresponding with control group 1 of embodiment 14 can in contrast table 2
To find out, material quantity under the same conditions, in raw material containing lead a cow flower extract more containing lead a cow flower extract can be obvious
Product is improved to the therapeutic effect of white hair.
Also, embodiment 1, embodiment 14 and control group 2 can be seen that the corresponding product of embodiment 1 in contrast table 2
The sum of curative effect of curative effect product corresponding with control group 2 is less than the curative effect of the corresponding product of embodiment 14.
The above results can illustrate that will lead a cow in the application has synergistic work between flower extract and original raw material
With the healing result effect that can further increase product to white hair under the conditions of flower extract of leading a cow is existing.
Test example 4
By taking embodiment 14, embodiment 17 and embodiment 18 as an example, contrast groups 1 and contrast groups 2, control group 1 and reality are set
The difference for applying example 14 is: the morning glory in contrast groups 1 is blush morning glory, and the morning glory in contrast groups 2 is led for pink
Niu Hua.
It is resulting to embodiment 14, embodiment 17, embodiment 18, contrast groups 1 and contrast groups 2 according to the method for test example 1
Ganoderma lucidum functional food carries out effect assessment, and the results are shown in Table 3.
3 therapeutic effect of table (example)
| Embodiment 14 | Embodiment 17 | Embodiment 18 | Contrast groups 1 | Contrast groups 2 | |
| It cures | 20 | 23 | 22 | 12 | 13 |
| It is significant effective | 12 | 9 | 10 | 20 | 15 |
| Effectively | 0 | 0 | 0 | 0 | 4 |
| In vain | 0 | 0 | 0 | 0 | 0 |
From the point of view of healing and significant effective number of cases, embodiment 14, embodiment 17, embodiment 18, contrast groups in contrast table 3
1 and the resulting ganoderma lucidum functional food of contrast groups 2 therapeutic effect can be seen that dosage under the same conditions, led in raw material
Ox flower extract is extracted by blue and/or purple morning glory and obtaining can obtain compared with being extracted by the morning glory of blush or pink
Product is more conducively improved to the healing result of white hair.
In conclusion ganoderma lucidum functional food formula provided by the present application is rationally, taste is preferable, white with significantly promoting
Hair turns the effect of dark hair, and quick.Preparation method is simple, can largely retain in material composition activity at
Point.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of ganoderma lucidum functional food, which is characterized in that including the first component and the second component；
First component is by the first complex enzyme formulation of 0.05-0.1 parts by weight to the ganoderma lucidum and 5- for including 5-25 parts by weight
First raw material of the black fungus of 15 parts by weight carries out the first enzymatic hydrolysis and is made；
Second component is by the second complex enzyme formulation of 0.05-0.1 parts by weight to semen sojae atricolor, 15- including 15-25 parts by weight
The Semen sesami nigrum of 25 parts by weight, the black rice of 5-15 parts by weight, the jujube of 5-15 parts by weight and 5-15 parts by weight mulberries it is second former
Material carries out the second enzymatic hydrolysis and is made；
Preferably, second enzymatic hydrolysis is carried out in the presence of the modulator of 0.1-0.2 parts by weight；
Preferably, first complex enzyme formulation includes cellulase, chitinase, papain and pectase；
Preferably, second complex enzyme formulation includes cellulase, mesophilicα-diastase, zytase and basic protein
Enzyme；
Preferably, the modulator includes calcium chloride, magnesium sulfate, zinc sulfate, sodium citrate and vitamin C；
Preferably, the semen sojae atricolor is the semen sojae atricolor through frying；The Semen sesami nigrum is the Semen sesami nigrum through frying；The black rice is through frying
Black rice.
2. functional food according to claim 1, which is characterized in that first component passes through 0.06-0.09 weight
Institute of first complex enzyme formulation of part to the ganoderma lucidum for including 18-22 parts by weight and the black fungus of 8-12 parts by weight
The first enzymatic hydrolysis of the first raw material progress is stated to be made；
Second component is by second complex enzyme formulation of 0.06-0.09 parts by weight to the institute including 18-22 parts by weight
State semen sojae atricolor, the Semen sesami nigrum of 17.5-22.5 parts by weight, the black rice of 8-12 parts by weight, 8-12 parts by weight the jujube
The second enzymatic hydrolysis is carried out with the second raw material of the mulberries of 7.5-12.5 parts by weight to be made；
Preferably, second enzymatic hydrolysis is carried out in the presence of the modulator of 0.14-0.16 parts by weight.
3. functional food according to claim 2, which is characterized in that first component passes through 0.075 parts by weight
First original of first complex enzyme formulation to the ganoderma lucidum for including 20 parts by weight and the black fungus of 10 parts by weight
Material carries out the first enzymatic hydrolysis and is made；
Second component by second complex enzyme formulations of 0.075 parts by weight to include 20 parts by weight the semen sojae atricolor,
The Semen sesami nigrum of 20 parts by weight, the black rice of 10 parts by weight, the jujube of 10 parts by weight and the mulberry of 10 parts by weight
The second raw material of Shen carries out the second enzymatic hydrolysis and is made；
Preferably, second enzymatic hydrolysis is carried out in the presence of the modulator of 0.15 parts by weight.
4. functional food according to claim 1-3, which is characterized in that institute in first complex enzyme formulation
The enzyme activity for stating cellulase is 20000-30000u/g, and the enzyme activity of the chitinase is 5000-6000u/g, the pawpaw egg
The enzyme activity of white enzyme is 20000-30000u/g, and the enzyme activity of the pectase is 5000-6000u/g.
5. functional food according to claim 1-3, which is characterized in that institute in second complex enzyme formulation
The enzyme activity for stating cellulase is 20000-30000u/g, and the enzyme activity of mesophilicα-diastase is 2000-3000u/g, the xylan
The activity of enzyme is 5000-6000u/g, and the activity of the alkali protease is 50000-60000u/g.
6. functional food according to claim 4, which is characterized in that calcium chloride described in the modulator, the sulphur
Weight ratio between sour magnesium, the zinc sulfate, the sodium citrate and the vitamin C is 18-22:4-6:4-6:35-45:
25-35；
Preferably, calcium chloride described in the modulator, the magnesium sulfate, the zinc sulfate, the sodium citrate and described
Weight ratio between vitamin C is 20:5:5:40:30.
7. functional food according to claim 1-3, which is characterized in that the functional food further includes 8-
The flower extract of leading a cow of 12 parts by weight；
The flower extract of leading a cow after methanol aqueous solution extracts, is filtered, by filtrate in macroporous adsorption resin chromatography by morning glory
Gradient elution in column collects third elution fraction, then purifies and obtain through preparative liquid chromatography；
The morning glory is blue morning glory and/or purple morning glory；
Preferably, the condition of gradient elution includes: using methanol-water solution as eluent, by the methanol in the eluent
Percentage by volume be followed successively by 0%, 20%, 40%, 60%, 80% and 100% ratio carry out gradient elution；
Preferably, the condition of preparative liquid chromatography purifying includes: using first alcohol and water as eluent, by the initial of the first alcohol and water
Volume ratio is 10:90, and final volume ratio is 80:20 gradient elution 30-40min, elution flow rate 18-20mL/min.
8. such as the preparation method of the described in any item functional foods of claim 1-7, which comprises the following steps:
First enzymatic hydrolysis is carried out to first raw material using first complex enzyme formulation, subsequent enzyme deactivation obtains the first enzymolysis liquid；
Second enzymatic hydrolysis is carried out to second raw material using second complex enzyme formulation, subsequent enzyme deactivation obtains the second enzymolysis liquid；
Mix first enzymolysis liquid and second enzymolysis liquid；
Preferably, the first enzymatic hydrolysis carries out 2-3h under conditions of 50-55 DEG C；
Preferably, the second enzymatic hydrolysis carries out 2-3h under conditions of 50-55 DEG C；
Preferably, second enzymatic hydrolysis is carried out in the presence of the modulator of 0.1-0.2 parts by weight.
9. preparation method according to claim 8, which is characterized in that further include: mixed mixed enzymolysis liquid is carried out
Vacuum concentration and spray drying；
Preferably, the vacuum degree of the vacuum concentration is 0.06-0.08MPa, and temperature is 70-80 DEG C；It is concentrated in vacuo to solid content
Content is 25-30wt%；
Preferably, spray drying carries out under conditions of inlet temperature is 180-200 DEG C, outlet temperature is 70-80 DEG C.
10. preparation method according to claim 9, which is characterized in that when in the raw material also containing leading a cow flower extract
When, by mixed enzymolysis object that the mixed enzymolysis liquid obtains after dry with dry after described in flower extract of leading a cow mix.
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| CN108323719A (en) * | 2017-11-23 | 2018-07-27 | 安徽秋果食品有限公司 | A kind of black coarse grain powder with kidney-tonifying and hair-blackening function |
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Application publication date: 20190215 |