CN109329374A - A kind of marine shellfish bacteriostasis, preservation agent and its application method - Google Patents
A kind of marine shellfish bacteriostasis, preservation agent and its application method Download PDFInfo
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- CN109329374A CN109329374A CN201811134916.0A CN201811134916A CN109329374A CN 109329374 A CN109329374 A CN 109329374A CN 201811134916 A CN201811134916 A CN 201811134916A CN 109329374 A CN109329374 A CN 109329374A
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- bacteriostasis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/02—Preserving by means of inorganic salts
- A23B4/027—Preserving by means of inorganic salts by inorganic salts other than kitchen salt, or mixtures thereof with organic compounds, e.g. biochemical compounds
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- Meat, Egg Or Seafood Products (AREA)
Abstract
Particularly, the present invention relates to a kind of for carrying out the agent liquid and its application method of bacteriostasis, preservation to marine shellfish.To solve the technological gap present in seashells bacteriostasis, preservation at present, a kind of the technical solution adopted by the present invention are as follows: seashells bacteriostasis, preservation agent, it is characterized in that, it uses the addition dry powder substance into solvent to be made by dissolution, wherein dry powder substance and the weight ratio of solvent are 1:80-150；The dry powder substance weight ratio ingredient is as follows: 5-10 parts of lysozyme；30-50 parts of L-cysteine；50-80 parts of chitosan；5-10 parts of beta carotene；60-100 parts of sodium alginate；5-10 parts of methyl jasmonate, the solvent is sodium chloride solution.The invention has the benefit that making the freshness date of marine shellfish more lasting, and bacteriostasis, preservation effect is obvious.
Description
Technical field
Particularly, the present invention relates to a kind of for carrying out the agent liquid and its application method of bacteriostasis, preservation to marine shellfish.
Background technique
Traditional marine shellfish bio-preservative is mainly to carry out bacteriostasis, preservation using the antisepsis of nisin,
But only preservation timeliness is longer in acid condition for nisin, however will affect marine shellfish under acid condition
Original local flavor and delicate flavour, influence the economic benefit of marine shellfish cultivation industry indirectly.
By retrieval, actually rare applied to the research of seashells bacteriostasis, preservation at present, CN108220262A is disclosed
A kind of marine low temperature glucose oxidase and its application in seafood freshing, the marine low temperature glucose oxidase originate from sea
Foreign Penicillium crustosum, (GenBank accession number MG983981).Preparation process is Penicillium
Crustosum is inoculated in fermentation medium, and fermented culture obtains fermentation liquid, and 8000r/min is centrifuged 20min after filtering thallus,
Ammonium sulfate precipitation is stayed overnight, centrifugation, and ultrafiltration after precipitating is redissolved using buffer crosses dextran chromatography column, obtains pure enzyme solution.This is fresh-keeping
Liquid be mainly used in prawn and hairtail it is fresh-keeping in, but seashells and prawn and hairtail have biggish difference, if are suitable for
The fresh-keeping of shellfish there is no conclusion.
Summary of the invention
To solve the technological gap present in seashells bacteriostasis, preservation at present, the present invention provide a kind of raw material be easy to get,
It is easy the bacteriostasis, preservation agent of preparation.
To realize the above-mentioned technical purpose, the technical solution adopted by the present invention are as follows: a kind of seashells bacteriostasis, preservation agent, it is special
Sign is, uses the addition dry powder substance into solvent to be made by dissolution, wherein dry powder substance and the weight ratio of solvent are 1:
80-150；The dry powder substance weight ratio ingredient is as follows: 5-10 parts of lysozyme；30-50 parts of L-cysteine；Chitosan 50-
80 parts；5-10 parts of beta carotene；60-100 parts of sodium alginate；5-10 parts of methyl jasmonate, the solvent is that sodium chloride is molten
Liquid.
Further, the concentration of the sodium chloride is 0.5-1.2%.
The application method of above-mentioned seashells bacteriostasis, preservation agent is that fresh shellfish is impregnated 10-30min in antistaling agent,
Shellfish is placed in crosslinking in calcium chloride solution after taking-up shellfish and impregnates 2-5min, pulls out, dries.
Further, the concentration of the chlorine calcium chloride solution is 3-8%.
The invention has the benefit that after fresh shellfish is impregnated in coating antistaling agent one can be formed in shellfish body surface
Coating film, and carry out crosslinking in calcium chloride solution and impregnate the toughness and resistance that can increase film, so that marine shellfish
Freshness date it is more lasting, and bacteriostasis, preservation effect is obvious.
Specific embodiment
Bacteriostasis, preservation agent provided by the invention and application method are described in detail below with reference to embodiment.
Embodiment 1
A kind of seashells bacteriostasis, preservation agent uses the interior addition dry powder substance into sodium chloride solution to be made by dissolution,
Middle concentration of sodium chloride solution is 0.9%, and the weight ratio of dry powder substance and sodium chloride solution is 1:100；The dry powder substance weight
It is as follows than component: 7 parts of lysozyme, 45 parts of L-cysteine, 60 parts of chitosan, 8 parts of beta carotene, 65 parts of sodium alginate, jasmine
6 parts of sour methyl esters.
The application method of the seafood bacteriostasis, preservation agent are as follows: fresh shellfish is impregnated into 15min in antistaling agent, after taking out shellfish
Shellfish is placed in crosslinking in calcium chloride solution and impregnates 3min, pulls out, dries.
Inventor is research object to commercially available new fresh scallops, oyster, mussel according to bacteriostasis, preservation agent provided in this embodiment,
The TVB-N content of traditional freeze preservation and fresh-keeping bacteriostatic coating problems after 2 months is detected respectively.
As a result as follows:
| Sample type | Traditional freeze preservation shellfish Volatile Base Nitrogen (mg/100g) | Fresh-keeping bacteriostatic coating-film fresh-keeping shellfish Volatile Base Nitrogen (mg/100g) |
| Scallop | 4.32 | 0.89 |
| Oyster | 3.84 | 0.16 |
| Mussel | 6.52 | 0.67 |
Volatile Base Nitrogen (TVB-N) refers to animal food due to the effect of enzyme and bacterium, in decay process, makes protein point
The alkaline nitrogen substance such as solve and generate ammonia and amine.Substance of this kind has volatility, and content is higher, shows that amino acid is broken
Bad is more, especially methionine and tyrosine, therefore nutritive value is greatly affected.Therefore pass through the content energy of measurement alkali nitrogen
Enough it is apparent from the bacteriostasis, preservation effect of the bacteriostasis, preservation agent.By the test to scallop, oyster, mussel, can clearly show that
Excellent performance is achieved using bacteriostasis, preservation of the bacteriostasis, preservation agent provided in this embodiment to above-mentioned three kinds of marine shellfishes, it can
The significant fresh keeping time for extending shellfish.
Embodiment 2
A kind of seashells bacteriostasis, preservation agent uses the interior addition dry powder substance into sodium chloride solution to be made by dissolution,
Middle concentration of sodium chloride solution is 0.5%, and the weight ratio of dry powder substance and sodium chloride solution is 1:150；The dry powder substance weight
It is as follows than component: 10 parts of lysozyme；30 parts of L-cysteine；80 parts of chitosan；5 parts of beta carotene；100 parts of sodium alginate；Jasmine
5 parts of jasmine acid methyl esters, the application method of the seafood bacteriostasis, preservation agent are as follows: fresh shellfish is impregnated into 10min in antistaling agent, takes out shellfish
Shellfish is placed in crosslinking in calcium chloride solution after class and impregnates 5min, pulls out, dries.
Inventor is research object to commercially available fresh abalone, razor clam, clam according to bacteriostasis, preservation agent provided in this embodiment, point
It Jian Ce not the TVB-N content of traditional freeze preservation and fresh-keeping bacteriostatic coating problems after 2 months.
As a result as follows:
| Sample type | Traditional freeze preservation shellfish Volatile Base Nitrogen (mg/100g) | Fresh-keeping bacteriostatic coating-film fresh-keeping shellfish Volatile Base Nitrogen (mg/100g) |
| Abalone | 6.69 | 0.94 |
| Razor clam | 6.01 | 0.05 |
| Clam | 3.12 | 0.08 |
By the test to abalone, razor clam, clam, can clearly show that using bacteriostasis, preservation agent provided in this embodiment to above-mentioned three
The bacteriostasis, preservation of kind marine shellfish achieves excellent performance, can significantly extend the fresh keeping time of shellfish.
Embodiment 3
A kind of seashells bacteriostasis, preservation agent uses the interior addition dry powder substance into sodium chloride solution to be made by dissolution,
Middle concentration of sodium chloride solution is 1.2%, and the weight ratio of dry powder substance and sodium chloride solution is 1:80；The dry powder substance weight
It is as follows than component: 5 parts of lysozyme；50 parts of L-cysteine；50 parts of chitosan；10 parts of beta carotene；60 parts of sodium alginate；Jasmine
10 parts of jasmine acid methyl esters, the application method of the seafood bacteriostasis, preservation agent are as follows: fresh shellfish is impregnated into 30min in antistaling agent, is taken out
Shellfish is placed in crosslinking in calcium chloride solution after shellfish and impregnates 2min, pulls out, dries.
Inventor is research object to commercially available fresh abalone, razor clam, clam according to bacteriostasis, preservation agent provided in this embodiment, point
It Jian Ce not the TVB-N content of traditional freeze preservation and fresh-keeping bacteriostatic coating problems after 2 months.
As a result as follows:
| Sample type | Traditional freeze preservation shellfish Volatile Base Nitrogen (mg/100g) | Fresh-keeping bacteriostatic coating-film fresh-keeping shellfish Volatile Base Nitrogen (mg/100g) |
| Blood clam | 2.06 | 0.006 |
| Emerald spiral shell | 4.47 | 0.23 |
| Byssus spiral shell | 4.98 | 0.11 |
| Buphthalmos clam | 7.82 | 0.51 |
By the test to blood clam, emerald spiral shell, byssus spiral shell, buphthalmos clam, can clearly show that using provided in this embodiment antibacterial
Antistaling agent achieves excellent performance to the bacteriostasis, preservation of above-mentioned four kinds of marine shellfishes, can significantly extend shellfish it is fresh-keeping when
Between.
Claims (4)
1. a kind of seashells bacteriostasis, preservation agent, which is characterized in that it, which is used, adds dry powder substance into solvent by dissolution system
At wherein dry powder substance and the weight ratio of solvent are 1:80-150；The dry powder substance weight ratio ingredient is as follows: lysozyme 5-
10 parts；30-50 parts of L-cysteine；50-80 parts of chitosan；5-10 parts of beta carotene；60-100 parts of sodium alginate；Jasmonic
5-10 parts of methyl esters, the solvent is sodium chloride solution.
2. a kind of seashells bacteriostasis, preservation agent according to claim 1, it is characterised in that: the concentration of the sodium chloride is
0.5-1.2%。
3. a kind of application method of seashells bacteriostasis, preservation agent described in claim 1, which is characterized in that fresh shellfish exists
10-30min is impregnated in antistaling agent, and shellfish is placed in crosslinking in calcium chloride solution after taking-up shellfish and impregnates 2-5min, pulls out, dries in the air
It is dry.
4. the application method of seashells bacteriostasis, preservation agent according to claim 3, which is characterized in that the chlorine chlorination
The concentration of calcium solution is 3-8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811134916.0A CN109329374A (en) | 2018-09-28 | 2018-09-28 | A kind of marine shellfish bacteriostasis, preservation agent and its application method |
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| CN201811134916.0A CN109329374A (en) | 2018-09-28 | 2018-09-28 | A kind of marine shellfish bacteriostasis, preservation agent and its application method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022151515A1 (en) * | 2021-01-13 | 2022-07-21 | 浙江海洋大学 | Method for preparing dried scallops |
| CN114947039A (en) * | 2022-05-18 | 2022-08-30 | 绥化市蓝源生物工程有限公司 | Compound anticorrosive composition based on L-amino acid polyglycoside, and preparation method and application thereof |
| CN115444031A (en) * | 2022-09-01 | 2022-12-09 | 陕西师范大学 | Protein-based fruit antibacterial fresh-keeping edible coating and preparation method and application thereof |
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| CN102578681A (en) * | 2011-01-17 | 2012-07-18 | 河北农业大学 | Shellfish film preservative |
| CN106962453A (en) * | 2017-04-27 | 2017-07-21 | 北海市万景海产有限公司 | A kind of freeze preservation method of instant natural car shrimp living |
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| CN102578681A (en) * | 2011-01-17 | 2012-07-18 | 河北农业大学 | Shellfish film preservative |
| CN106962453A (en) * | 2017-04-27 | 2017-07-21 | 北海市万景海产有限公司 | A kind of freeze preservation method of instant natural car shrimp living |
| CN107751347A (en) * | 2017-10-09 | 2018-03-06 | 浙江海洋大学 | A kind of polyphenol oxidase passivating method based on dielectric barrier discharge low-temperature plasma |
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| 郑惠娜，等: "文蛤肌肉水溶性提取物褐变及其抑制研究", 《中国食品学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022151515A1 (en) * | 2021-01-13 | 2022-07-21 | 浙江海洋大学 | Method for preparing dried scallops |
| CN114947039A (en) * | 2022-05-18 | 2022-08-30 | 绥化市蓝源生物工程有限公司 | Compound anticorrosive composition based on L-amino acid polyglycoside, and preparation method and application thereof |
| CN115444031A (en) * | 2022-09-01 | 2022-12-09 | 陕西师范大学 | Protein-based fruit antibacterial fresh-keeping edible coating and preparation method and application thereof |
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