CN109321614A - It is a kind of to promote the composition and its application that vitamin B12 ferments - Google Patents
It is a kind of to promote the composition and its application that vitamin B12 ferments Download PDFInfo
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- CN109321614A CN109321614A CN201811232188.7A CN201811232188A CN109321614A CN 109321614 A CN109321614 A CN 109321614A CN 201811232188 A CN201811232188 A CN 201811232188A CN 109321614 A CN109321614 A CN 109321614A
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- fermentation
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- thallus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
Abstract
The present invention relates to the preparation fields of vitamin B12, and in particular to a kind of to promote the composition and its application that vitamin B12 ferments.This kind promotees the composition of vitamin B12 fermentation, is mainly made of the raw material of following mass fraction: composite precursor 1-1.55%, compounded amino organic acid 2-10%, composite thallus hydrolyzate 30-40% and tap water 50-70%.A kind of composition of rush vitamin B12 fermentation of the invention and its application, solve the problems, such as fermentation shakiness, low output, greatly improve fermentation yield, realize the purpose of energy consumption reduction, clean manufacturing, when that will promote vitamin B12 fermentation composition and be applied in vitamin B12 fermentation process, rush fermentation composition is constantly added during the fermentation, ensure that the supply of various nutritional ingredients in fermentation process, it ensure that ferment effect, effectively increase the fermentation yield of vitamin B12.
Description
Technical field
The present invention relates to the preparation fields of vitamin B12, and in particular to it is a kind of promote vitamin B12 fermentation composition and its
Using.
Background technique
Vitamin B12 is cobalt aminated compounds, is clinically mainly used for treating pernicious anaemia, while being also used for treating various
The nervous system disease and certain liver diseases etc..
Vitamin B12 relies primarily on Pseuomonas denitrifican and is made by aerobic process fermentation at present.The master of fermentation medium
Wanting ingredient includes organic carbon source, organic nitrogen source, glycine betaine, a small amount of inorganic salts etc..Wherein microelement subsidiary in organic matter battalion
The feeding factor is normal function and the indispensable factor of metabolic activity, and the regulation of many microbial metabolisms is participated in as co-factor,
It is a key link in microorganism metabolism controlling, the co-factor of different level can change the size of specific enzyme activity, from
And redistribute the flow direction of carbon skeleton substance in the cell.In addition, the micro-element nutrition factor also participates in adjusting the enzyme of respiratory metabolism
Activity improves microorganism to the utilization rate of oxygen as cofactors.But in microbial fermentation production, different manufacturers, difference
The content of trophic factors is irregular in organic culture that time, different process produce, and has seriously affected the control of zymotechnique
System and ferment effect.
In addition, a large amount of mycoprotein can be generated in existing vitamin B12 production process, these mycoprotein added values
It is not high, be all the time it is dry after sold as feed, wet thallus causes mildew to be made because being not easy storage in process of production
At great waste and it polluted environment, and it is even more big power consumer that thallus is dry, is unfavorable for the policy of national energy conservation and emission reduction.
Summary of the invention
The purpose of the present invention is to provide a kind of composition of rush vitamin B12 fermentation and its applications, overcome aforementioned existing
The deficiency of technology provides a kind of alimentation composition that application promotes the strain growth in fermentation to act on, while can guarantee various micro-
The supply of secondary element, reduce because in organic carbon nitrogen source microelement it is unstable caused by ferment problem, can effectively promote fermentation and produce
The rush vitamin B12 composition of amount, the fermentation nitrogen source using sulphuric acid hydrolysis thallus as vitamin B12 realize the circulation of resource
It utilizes, to realize the purpose of energy consumption reduction, clean manufacturing.
The technical scheme adopted by the invention to solve the technical problem is that:
A kind of composition promoting vitamin B12 fermentation, is mainly made of the raw material of following mass fraction: composite precursor 1-1.55%,
Compounded amino organic acid 2-10%, composite thallus hydrolyzate 30-40% and tap water 50-70%；The composite precursor is mainly by 5,6-
Dimethylbenzimidazole, nicotinamide mononucleotide and glycine betaine 1:2:2 in mass ratio composition；The compounded amino organic acid is main
It is made of threonine, glycine and citric acid 2-5:1-3:1-3.5 in mass ratio；The composite thallus hydrolyzate is mainly by denitrogenation
Pseudomonad hydrolyzate and yeast extract 2-3:5-8 in mass ratio composition.
Preferably, 5, the 6- dimethylbenzimidazole active constituent content is 90-98%, the nicotinamide mononucleotide
Active constituent content is 90-95%, and the glycine betaine active constituent content is 90-98%, the threonine, glycine, citric acid
Active constituent content >=95%.
Preferably, the content of impurity trichlorine phosphoric acid is 0.001-0.1wt% in the nicotinamide mononucleotide.
Preferably, the Pseuomonas denitrifican hydrolyzate the preparation method comprises the following steps: select plate-frame filtering after denitrogenation vacation unit cell
Bacterium wet thallus, in Pseuomonas denitrifican wet thallus: the 98% content concentrated sulfuric acid is the ratio of 50:1, and steam adds in ceramic reactor
Heat boiling 10-30min, mixed after cooling with yeast extract to get.
A kind of application of the composition promoting vitamin B12 fermentation in vitamin B12 fermentation, vitamin B12 fermentation
The following steps are included:
(1) preparation of seed culture medium: the prescription quality of the seed culture medium is than composition are as follows: maltose 3.0-5.0%, corn
Starch 1-1.5%, ammonium sulfate 0.05-0.15%, diammonium hydrogen phosphate 0.05-0.25%, manganese sulfate 0.08-0.1%, cobalt chloride 0.001-
0.003%, magnesia 0.01-0.03%, zinc sulfate 0.0005-0.0015%, calcium carbonate 0.1-0.2% and tap water 90-95%, institute
The pH for stating seed culture medium is 7.2-7.4；
(2) preparation of fermentation medium: the prescription quality of the step fermentation medium is than composition are as follows: maltose 10-12%, jade
Rice & peanut milk 1-2%, ammonium sulfate 0.05-0.1%, potassium dihydrogen phosphate 0.04-0.07%, cobalt chloride 0.006-0.008%, magnesia 0.04-
0.05%, zinc sulfate 0.005-0.008%, calcium carbonate 0.05-0.12% and tap water 80-90%, the pH of the fermentation medium are
7.5-8.0；
(3) seed culture: by seed culture medium sterilizing, the cooling of step (1), being inoculated with and produce strain into Pseuomonas denitrifican, in
30-32 DEG C of shaken cultivation OD value OD700 of thallus into strain seed liquor reaches 0.2-0.3；
(4) fermented and cultured: the fermented and cultured of step (2) is based on 121 DEG C of sterilizing 30min, by the rush vitamin B12 fermentation group
It closes object to be added in fermentation medium according to the additive amount of 2.0-4.0wt%, then be inoculated with according to the inoculum concentration of 10-15% into step
Suddenly the strain seed liquor in (1) adds the rush vitamin B12 in 30-32 DEG C of progress aerobic fermentation culture during the fermentation
Fermentation composition periodically detects beet alkali content during adding, and maintaining the content of glycine betaine in fermentation liquid is 0.2-0.8%,
Fermentation time 1-9 days.
The beneficial effects of the present invention are: compared with prior art, a kind of composition of rush vitamin B12 fermentation of the invention
And its application has the advantages that (1) present invention promotees the various micronutrient factor additive of fermentation composition, meets
The appropriate combination supply of fermentation needed nutrient, solves the problems, such as fermentation shakiness, low output, greatly improves fermentation yield.
(2) present invention promotees to use sulphuric acid hydrolysis thallus as the fermentation nitrogen source of vitamin B12, it can be achieved that following for resource in fermentation composition
Ring utilizes, to realize the purpose of energy consumption reduction, clean manufacturing.(3) present invention is answered will promote vitamin B12 fermentation composition
When using in vitamin B12 fermentation process, rush fermentation composition is constantly added during the fermentation, ensure that each in fermentation process
The supply of kind nutritional ingredient, ensure that ferment effect, effectively increases the fermentation yield of vitamin B12.
Specific embodiment
A kind of composition for promoting vitamin B12 fermentation of embodiment 1
A kind of composition promoting vitamin B12 fermentation, is mainly made of the raw material of following mass fraction: composite precursor 1.5kg is multiple
Close amino organic acid 5kg, composite thallus hydrolyzate 35kg and tap water 58.5kg；The composite precursor is mainly by 5,6- dimethyl
Benzimidazole, nicotinamide mononucleotide and glycine betaine 1:2:2 in mass ratio composition；The compounded amino organic acid is mainly by Soviet Union's ammonia
Acid, glycine and citric acid 5:2.4:1.6 in mass ratio composition；The composite thallus hydrolyzate is mainly by Pseuomonas denitrifican water
Solve liquid and yeast extract 1:4 in mass ratio composition.5, the 6- dimethylbenzimidazole active constituent content is 95%, the nicotinoyl
Amine mononucleotide active constituent content is 92%, and the glycine betaine active constituent content is 96%, the threonine, glycine, lemon
>=95%, the content of impurity trichlorine phosphoric acid is 0.01wt% to the active constituent content of lemon acid in the nicotinamide mononucleotide.
The Pseuomonas denitrifican hydrolyzate the preparation method comprises the following steps: select plate-frame filtering after the wet bacterium of Pseuomonas denitrifican
Body, in Pseuomonas denitrifican wet thallus: the 98% content concentrated sulfuric acid is the ratio of 50:1, the steam ebuillition of heated in ceramic reactor
15min, mixed after cooling with yeast extract to get.
A kind of composition for promoting vitamin B12 fermentation of embodiment 2
A kind of composition promoting vitamin B12 fermentation, is mainly made of the raw material of following mass fraction: composite precursor 1.2kg is multiple
Close amino organic acid 7kg, composite thallus hydrolyzate 32kg and tap water 60.8kg；The composite precursor is mainly by 5,6- dimethyl
Benzimidazole, nicotinamide mononucleotide and glycine betaine 1:2:2 in mass ratio composition；The compounded amino organic acid is mainly by Soviet Union's ammonia
Acid, glycine and citric acid 3.4:2:1.6 in mass ratio composition；The composite thallus hydrolyzate is mainly by Pseuomonas denitrifican water
Solve liquid and yeast extract 1:3 in mass ratio composition.5, the 6- dimethylbenzimidazole active constituent content is 93%, the nicotinoyl
Amine mononucleotide active constituent content is 94%, and the glycine betaine active constituent content is 94%, the threonine, glycine, lemon
>=95%, the content of impurity trichlorine phosphoric acid is 0.05wt% to the active constituent content of lemon acid in the nicotinamide mononucleotide.
The Pseuomonas denitrifican hydrolyzate the preparation method comprises the following steps: select plate-frame filtering after the wet bacterium of Pseuomonas denitrifican
Body, in Pseuomonas denitrifican wet thallus: the 98% content concentrated sulfuric acid is the ratio of 50:1, the steam ebuillition of heated in ceramic reactor
10min, mixed after cooling with yeast extract to get.
A kind of composition for promoting vitamin B12 fermentation of embodiment 3
A kind of composition promoting vitamin B12 fermentation, is mainly made of the raw material of following mass fraction: composite precursor 1.4kg is multiple
Close amino organic acid 9kg, composite thallus hydrolyzate 38kg and tap water 51.6kg；The composite precursor is mainly by 5,6- dimethyl
Benzimidazole, nicotinamide mononucleotide and glycine betaine 1:2:2 in mass ratio composition；The compounded amino organic acid is mainly by Soviet Union's ammonia
Acid, glycine and citric acid 4:2.5:2.5 in mass ratio composition；The composite thallus hydrolyzate is mainly by Pseuomonas denitrifican water
Solve liquid and yeast extract 1:2.8 in mass ratio composition.5, the 6- dimethylbenzimidazole active constituent content is 94%, the cigarette
Amide mononucleotide active constituent content be 91%, the glycine betaine active constituent content be 95%, the threonine, glycine,
>=95%, the content of impurity trichlorine phosphoric acid is 0.001wt% to the active constituent content of citric acid in the nicotinamide mononucleotide.
The Pseuomonas denitrifican hydrolyzate the preparation method comprises the following steps: select plate-frame filtering after the wet bacterium of Pseuomonas denitrifican
Body, in Pseuomonas denitrifican wet thallus: the 98% content concentrated sulfuric acid is the ratio of 50:1, the steam ebuillition of heated in ceramic reactor
10min, mixed after cooling with yeast extract to get.
A kind of application of the composition for promoting vitamin B12 fermentation of embodiment 4 in vitamin B12 fermentation
A kind of application method of the composition promoting vitamin B12 fermentation in vitamin B12 fermentation, vitamin B12 fermentation
The following steps are included:
(1) preparation of seed culture medium: the prescription quality of the seed culture medium is than composition are as follows: maltose 30g, corn pulp 10g,
Ammonium sulfate 0.5g, diammonium hydrogen phosphate 0.5g, manganese sulfate 0.8g, cobalt chloride 0.01g, magnesia 0.1g, zinc sulfate 0.005g, carbonic acid
Calcium 1g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the seed culture medium is 7.2；
(2) preparation of fermentation medium: the prescription quality of the step fermentation medium is than composition are as follows: maltose 105g, corn
Starch 20g, ammonium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, cobalt chloride 0.06g, magnesia 0.4g, zinc sulfate 0.06g, calcium carbonate
0.8g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the fermentation medium is 7.8；
(3) seed culture, liquid amount 60ml, by the seed culture medium of step (1) seed culture: are carried out in the shaking flask of 300ml
It is sterilizing, cooling, be inoculated with and produce strain into Pseuomonas denitrifican, in 32 DEG C, 220rpm shaken cultivation into strain seed liquor thallus
OD value OD700 reach 0.26；
(4) the rush vitamin B12 fermentation composition additive amount in 25g embodiment 1 is added to the fermentation of step (2) by fermented and cultured
In culture medium, fermented and cultured is then based on 121 DEG C of sterilizing 30min, sterilize 30min at 122 DEG C, is then inoculated with into step
(1) the strain seed liquor 2.7ml in, is vibrated, aerobic fermentation culture in 32 DEG C, 220rpm, adds institute during the fermentation
It states and promotees vitamin B12 fermentation composition, beet alkali content is periodically detected during adding, glycine betaine contains in maintenance fermentation liquid
Amount is 0.5-0.8%.
The present embodiment fermentation period is 182h, and fermentation ends, the fermentation titer of vitamin B12 is 238mg/L.
A kind of application of the composition for promoting vitamin B12 fermentation of embodiment 5 in vitamin B12 fermentation
A kind of application method of the composition promoting vitamin B12 fermentation in vitamin B12 fermentation, vitamin B12 fermentation
The following steps are included:
(1) preparation of seed culture medium: the prescription quality of the seed culture medium is than composition are as follows: maltose 40g, corn pulp 15g,
Ammonium sulfate 0.6g, diammonium hydrogen phosphate 0.6g, manganese sulfate 0.6g, cobalt chloride 0.02g, magnesia 0.2g, zinc sulfate 0.006g, carbonic acid
Calcium 1.5g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the seed culture medium is 7.3；
(2) preparation of fermentation medium: the prescription quality of the step fermentation medium is than composition are as follows: maltose 100g, corn
Starch 18g, ammonium sulfate 0.6g, potassium dihydrogen phosphate 0.7g, cobalt chloride 0.08g, magnesia 0.5g, zinc sulfate 0.07g, calcium carbonate
0.9g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the fermentation medium is 7.9；
(3) seed culture, liquid amount 60ml, by the seed culture medium of step (1) seed culture: are carried out in the shaking flask of 300ml
It is sterilizing, cooling, be inoculated with and produce strain into Pseuomonas denitrifican, in 33 DEG C, 220rpm shaken cultivation into strain seed liquor thallus
OD value OD700 reach 0.24；
(4) the rush vitamin B12 fermentation composition additive amount in 35g embodiment 2 fermented and cultured: is added to the hair of step (2)
In ferment culture medium, fermented and cultured is based on 121 DEG C of sterilizing 35min, the strain seed liquor 3.6ml being then inoculated in step (1),
It is vibrated in 33 DEG C, 260rpm, aerobic fermentation culture, adds the rush vitamin B12 fermentation composition during the fermentation,
Beet alkali content is periodically detected during adding, maintaining the content of glycine betaine in fermentation liquid is 0.3-0.5%.
The present embodiment fermentation period is 178h, and fermentation ends, the fermentation titer of vitamin B12 is 263mg/L.
A kind of application of the composition for promoting vitamin B12 fermentation of embodiment 6 in vitamin B12 fermentation
A kind of application method of the composition promoting vitamin B12 fermentation in vitamin B12 fermentation, vitamin B12 fermentation
The following steps are included:
(1) preparation of seed culture medium: the prescription quality of the seed culture medium is than composition are as follows: maltose 40g, corn pulp 15g,
Ammonium sulfate 0.6g, diammonium hydrogen phosphate 0.6g, manganese sulfate 0.6g, cobalt chloride 0.02g, magnesia 0.2g, zinc sulfate 0.006g, carbonic acid
Calcium 1.5g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the seed culture medium is 7.3；
(2) preparation of fermentation medium: the prescription quality of the step fermentation medium is than composition are as follows: maltose 100g, corn
Starch 18g, ammonium sulfate 0.6g, potassium dihydrogen phosphate 0.7g, cobalt chloride 0.08g, magnesia 0.5g, zinc sulfate 0.07g, calcium carbonate
0.9g, above-mentioned raw materials are settled to 1000ml with tap water, and the pH of the fermentation medium is 7.9；
(3) seed culture, liquid amount 60ml, by the seed culture medium of step (1) seed culture: are carried out in the shaking flask of 300ml
It is sterilizing, cooling, be inoculated with and produce strain into Pseuomonas denitrifican, in 33 DEG C, 220rpm shaken cultivation into strain seed liquor thallus
OD value OD700 reach 0.24；
(4) the rush vitamin B12 fermentation composition additive amount in 35g embodiment 3 fermented and cultured: is added to the hair of step (2)
In ferment culture medium, fermented and cultured is based on 121 DEG C of sterilizing 35min, the strain seed liquor 3.6ml being then inoculated in step (1),
It is vibrated in 33 DEG C, 260rpm, aerobic fermentation culture, adds the rush vitamin B12 fermentation composition during the fermentation,
Beet alkali content is periodically detected during adding, maintaining the content of glycine betaine in fermentation liquid is 0.3-0.5%.
The present embodiment fermentation period is 168h, and fermentation ends, the fermentation titer of vitamin B12 is 255mg/L.
Above-mentioned specific embodiment is only specific case of the invention, and scope of patent protection of the invention includes but is not limited to
The product form and style of above-mentioned specific embodiment, any claims of the present invention and any technical field of meeting
The appropriate changes or modifications that those of ordinary skill does it, all shall fall within the protection scope of the present invention.
Claims (5)
1. a kind of composition for promoting vitamin B12 fermentation, it is characterised in that: be mainly made of the raw material of following mass fraction: multiple
Close precursor 1-1.55%, compounded amino organic acid 2-10%, composite thallus hydrolyzate 30-40% and tap water 50-70%；It is described compound
Precursor is mainly made of 5,6- dimethylbenzimidazole, nicotinamide mononucleotide and glycine betaine 1:2:2 in mass ratio；It is described compound
Amino organic acid is mainly made of threonine, glycine and citric acid 2-5:1-3:1-3.5 in mass ratio；The composite thallus water
Solution liquid is mainly made of Pseuomonas denitrifican hydrolyzate and yeast extract 2-3:5-8 in mass ratio.
2. a kind of composition for promoting vitamin B12 fermentation as described in claim 1, it is characterised in that: 5, the 6- dimethyl
Benzimidazole active constituent content is 90-98%, and the nicotinamide mononucleotide active constituent content is 90-95%, the beet
Alkali active constituent content be 90-98%, the threonine, glycine, citric acid active constituent content >=95%.
3. a kind of composition for promoting vitamin B12 fermentation as described in claim 1, it is characterised in that: the niacinamide monokaryon
The content of impurity trichlorine phosphoric acid is 0.001-0.1wt% in thuja acid.
4. a kind of composition for promoting vitamin B12 fermentation as described in claim 1, it is characterised in that: the denitrogenation vacation unit cell
Bacterium hydrolyzate the preparation method comprises the following steps: select plate-frame filtering after Pseuomonas denitrifican wet thallus, by Pseuomonas denitrifican wet thallus:
The 98% content concentrated sulfuric acid is the ratio of 50:1, and the steam ebuillition of heated 10-30min in ceramic reactor is mixed with yeast extract after cooling
Close to get.
5. a kind of application of the composition for promoting vitamin B12 fermentation as described in claim 1 in vitamin B12 fermentation,
Be characterized in that: vitamin B12 fermentation the following steps are included:
(1) preparation of seed culture medium: the prescription quality of the seed culture medium is than composition are as follows: maltose 3.0-5.0%, corn
Starch 1-1.5%, ammonium sulfate 0.05-0.15%, diammonium hydrogen phosphate 0.05-0.25%, manganese sulfate 0.08-0.1%, cobalt chloride 0.001-
0.003%, magnesia 0.01-0.03%, zinc sulfate 0.0005-0.0015%, calcium carbonate 0.1-0.2% and tap water 90-95%, institute
The pH for stating seed culture medium is 7.2-7.4；
(2) preparation of fermentation medium: the prescription quality of the step fermentation medium is than composition are as follows: maltose 10-12%, jade
Rice & peanut milk 1-2%, ammonium sulfate 0.05-0.1%, potassium dihydrogen phosphate 0.04-0.07%, cobalt chloride 0.006-0.008%, magnesia 0.04-
0.05%, zinc sulfate 0.005-0.008%, calcium carbonate 0.05-0.12% and tap water 80-90%, the pH of the fermentation medium are
7.5-8.0；
(3) seed culture: by seed culture medium sterilizing, the cooling of step (1), being inoculated with and produce strain into Pseuomonas denitrifican, in
30-32 DEG C of shaken cultivation OD value OD700 of thallus into strain seed liquor reaches 0.2-0.3；
(4) the rush vitamin B12 fermentation composition fermented and cultured: is added to step according to the additive amount of 2.0-4.0wt%
(2) in fermentation medium, fermented and cultured is then based on 121 DEG C of sterilizing 30min, is then connect according to the inoculum concentration of 10-15%
Kind enters the strain seed liquor in step (1), in 30-32 DEG C of progress aerobic fermentation culture, adds the rush dimension during the fermentation
Raw element B12 fermentation composition periodically detects beet alkali content during adding, and the content of glycine betaine is in maintenance fermentation liquid
0.2-0.8%, fermentation time 1-9 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205350A (en) * | 2019-05-24 | 2019-09-06 | 河北华荣制药有限公司 | One kind improving vitamin B based on the regulation of ammonia nitrogen index12The method of yield |
| CN110241166A (en) * | 2019-06-20 | 2019-09-17 | 山东泓达生物科技有限公司 | It is a kind of promote riboflavin fermentation composition and its application |
| CN111073922A (en) * | 2019-12-26 | 2020-04-28 | 湖北广济药业股份有限公司 | Vitamin B12Fermentation supplementing medium and supplementing method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1543026A1 (en) * | 2002-09-27 | 2005-06-22 | DSM IP Assets B.V. | Transcriptional activator gene for genes involved in cobalamin biosynthesis |
| CN102453740A (en) * | 2011-12-15 | 2012-05-16 | 宁夏多维药业有限公司 | Culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and fermentation method thereof |
| CN102719504A (en) * | 2012-07-04 | 2012-10-10 | 潍坊祥维斯化学品有限公司 | Vitamin B12 complex fermenting addition agent and application method thereof to fermenting vitamin B12 |
-
2018
- 2018-10-22 CN CN201811232188.7A patent/CN109321614A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1543026A1 (en) * | 2002-09-27 | 2005-06-22 | DSM IP Assets B.V. | Transcriptional activator gene for genes involved in cobalamin biosynthesis |
| CN102453740A (en) * | 2011-12-15 | 2012-05-16 | 宁夏多维药业有限公司 | Culture medium for producing vitamin B12 by fermenting pseudomonas denitrificans and fermentation method thereof |
| CN102719504A (en) * | 2012-07-04 | 2012-10-10 | 潍坊祥维斯化学品有限公司 | Vitamin B12 complex fermenting addition agent and application method thereof to fermenting vitamin B12 |
Non-Patent Citations (1)
| Title |
|---|
| 赵琳琳: "脱氮假单胞菌产维生素B_(12)发酵过程中甜菜碱作用机理及其补料策略的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205350A (en) * | 2019-05-24 | 2019-09-06 | 河北华荣制药有限公司 | One kind improving vitamin B based on the regulation of ammonia nitrogen index12The method of yield |
| CN110241166A (en) * | 2019-06-20 | 2019-09-17 | 山东泓达生物科技有限公司 | It is a kind of promote riboflavin fermentation composition and its application |
| CN111073922A (en) * | 2019-12-26 | 2020-04-28 | 湖北广济药业股份有限公司 | Vitamin B12Fermentation supplementing medium and supplementing method |
| CN111073922B (en) * | 2019-12-26 | 2023-04-07 | 湖北广济药业股份有限公司 | Vitamin B 12 Fermentation supplementing medium and supplementing method |
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