CN109321431A - A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application - Google Patents
A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application Download PDFInfo
- Publication number
- CN109321431A CN109321431A CN201811423915.8A CN201811423915A CN109321431A CN 109321431 A CN109321431 A CN 109321431A CN 201811423915 A CN201811423915 A CN 201811423915A CN 109321431 A CN109321431 A CN 109321431A
- Authority
- CN
- China
- Prior art keywords
- electrode
- solution
- enzyme
- nano
- nano material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 208000031225 myocardial ischemia Diseases 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 238000003745 diagnosis Methods 0.000 title claims abstract description 44
- 102000004190 Enzymes Human genes 0.000 claims abstract description 165
- 108090000790 Enzymes Proteins 0.000 claims abstract description 165
- 239000002086 nanomaterial Substances 0.000 claims abstract description 131
- 239000000758 substrate Substances 0.000 claims abstract description 114
- 150000001875 compounds Chemical class 0.000 claims abstract description 30
- 239000004020 conductor Substances 0.000 claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 167
- 229910021389 graphene Inorganic materials 0.000 claims description 97
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 63
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 54
- 239000002002 slurry Substances 0.000 claims description 50
- 239000002131 composite material Substances 0.000 claims description 48
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 36
- 229910052709 silver Inorganic materials 0.000 claims description 36
- 239000004332 silver Substances 0.000 claims description 36
- 229910052799 carbon Inorganic materials 0.000 claims description 28
- 239000007853 buffer solution Substances 0.000 claims description 27
- 239000003550 marker Substances 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 21
- 102000004625 Aspartate Aminotransferases Human genes 0.000 claims description 20
- 108010003415 Aspartate Aminotransferases Proteins 0.000 claims description 20
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 17
- 238000001548 drop coating Methods 0.000 claims description 16
- 239000000872 buffer Substances 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 238000007650 screen-printing Methods 0.000 claims description 9
- 150000001336 alkenes Chemical class 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims 1
- 239000004575 stone Substances 0.000 claims 1
- 230000004044 response Effects 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000002848 electrochemical method Methods 0.000 abstract description 3
- 239000008280 blood Substances 0.000 description 15
- 210000004369 blood Anatomy 0.000 description 15
- 238000012360 testing method Methods 0.000 description 10
- 229910002804 graphite Inorganic materials 0.000 description 6
- 239000010439 graphite Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241000209094 Oryza Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 229910017052 cobalt Inorganic materials 0.000 description 4
- 239000010941 cobalt Substances 0.000 description 4
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000005842 biochemical reaction Methods 0.000 description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 3
- 229910001429 cobalt ion Inorganic materials 0.000 description 3
- 239000007772 electrode material Substances 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 238000005253 cladding Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 108010023015 ischemia-modified albumin Proteins 0.000 description 2
- 230000000302 ischemic effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000004165 myocardium Anatomy 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 238000000554 physical therapy Methods 0.000 description 2
- 238000006479 redox reaction Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- BFXUWDKAQDARCA-DKWTVANSSA-N (2s)-2-aminobutanedioic acid;azane Chemical compound [NH4+].OC(=O)[C@@H](N)CC([O-])=O BFXUWDKAQDARCA-DKWTVANSSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 101710128063 Carbohydrate oxidase Proteins 0.000 description 1
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 1
- 229910004835 Na2B4O7 Inorganic materials 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000011982 device technology Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
Abstract
The invention discloses a kind of electrode sheet devices of quick diagnosis myocardial ischemia, including substrate, it is provided with signal on the substrate and generates area, the signal, which generates, to be provided with to electrode, reference electrode and working electrode in area, is provided with the contact conductor to connect one to one with each electrode on the substrate；The working electrode includes one or more of enzyme electrode, nano material combination electrode and the compound enzyme electrode of nano material；In addition, the invention also discloses a kind of preparation methods of electrode sheet devices；Further, the invention also discloses a kind of application of the electrode sheet devices in diagnosis of myocardial ischemia.For electrode sheet devices of the invention based on the enzyme on working electrode and/or the response between nano material and tested substance, being converted biochemical signals to using electrode sheet devices can be by the electric signal of electrochemical method determining, so that the detection of IMA is more rapidly efficient；Electrode sheet devices preparation method of the invention is simple, performance is stable, specificity and high sensitivity.
Description
Technical field
The invention belongs to in-vitro diagnosis medical device technologies, and in particular to a kind of electrode of quick diagnosis myocardial ischemia is on chip
Set and its preparation method and application.
Background technique
Myocardial ischemia will cause the illnesss such as angina pectoris, myocardial infarction, while can also induce other diseases, if cannot get and
When diagnosis and treatment, it is most likely that jeopardize patients ' lives.Accurate Diagnosis and treatment early stage myocardial ischemia can greatly reduce sufferer because of cardiac muscle
Dead probability caused by ischemic delay treatment.
In diagnosis of myocardial ischemia, ischemia modified albumin IMA (Ischemia modified albumin, IMA) is reflection
The marker of early stage myocardial ischemia, currently in clinical application, usually using Cobalt binding test (albumin-cobalt
Binding, ACB) treating myocardial ischemia damage to be evaluated, Cobalt binding test common method is determination of absorbance, with
The cobalt ions binding ability of existing albumin is comparison, IMA combination transition in an active in the serum specimen of normal control
The ability of metal cobalt ions reduces, and free cobalt ions can generate red with dithiothreitol (DTT) and react, color intensity and IMA content at
Direct ratio, by measuring the absorbance of red solution, the content of IMA in indirect determination blood.Cobalt binding test needs head
First normal value is determined, and establishes that experimentation is complex, while the stability of the dithiothreitol (DTT) as coloring agent
It is poor, it is not easy to maintain.
With the continuous development of medical technology, is cooperated by the electrode sheet devices and detecting instrument of specific function and done for human body
Corresponding inspection or physical therapy, are increasingly becoming the trend of physical therapy development.
In electrode sheet devices, the sensing unit adhered on working electrode can identify detected material and not send out with other substances
Raw effect, for enzyme because sensitivity with higher and specificity are passed through frequently as sensing unit, common binding mode is oxidation
Or reduction reaction, redox reaction can be by electrochemical method determinings；In addition, as researcher is to the depth of nano materials research
Enter, has and show unique biochemical property simultaneously compared with the nanoparticle of small particle and very big specific surface area, it will be conductive
The nano material and biological enzyme of energy are fixed on electrode surface and are prepared into the extensive concern that enzyme electrode sensor is similarly subjected to people.
In research at home and abroad and clinical application, it is at present common that electrode sheet devices, which are applied to blood glucose or sundry item test,
Detection means, device generally comprise contacting blood and transmission signal process.The detection mechanism of blood sugar test paper mainly passes through Portugal
Redox reaction occurs for the glucose in grape carbohydrate oxidase or glucose dehydrogenase and blood, and the electronics of generation is effectively counted
After number, through Instrument measuring and blood glucose value is converted to.The universal test for greatly facilitating blood glucose of blood sugar test paper, price and detection
Result is that people are received.However, it has been reported that test paper or other electrode sheet devices cannot be used to detect the acute heart
Myocardial ischemia index IMA.
Therefore, the diagnosis that a kind of diagnosis of myocardial ischemia electrode sheet devices are used for myocardial ischemia is invented, its is made to can recognize cardiac muscle
The marker IMA of ischemic becomes current technical problem urgently to be resolved.
Summary of the invention
Technical problem to be solved by the present invention lies in view of the above shortcomings of the prior art, provide a kind of quick diagnosis heart
Electrode sheet devices of myocardial ischemia and its preparation method and application.The electrode sheet devices are based on the enzyme and/or nanometer on working electrode
Response between material and tested substance, electrochemical method determining can be passed through by being converted biochemical signals to using electrode sheet devices
Electric signal so that the detection of IMA is more rapidly efficient；Electrode sheet devices preparation method is simple, performance is stable for this, specificity and spirit
Sensitivity is high；Further, it is also possible to realize while detecting the purpose of many indexes by changing working electrode channel.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of electrode of quick diagnosis myocardial ischemia
Sheet devices, including substrate, which is characterized in that be provided with signal on the substrate and generate area, the signal generates to be provided in area
To electrode, reference electrode and working electrode, the contact conductor to connect one to one with each electrode is provided on the substrate；It is described
Working electrode includes one or more of enzyme electrode, nano material combination electrode and the compound enzyme electrode of nano material.
The electrode sheet devices of above-mentioned a kind of quick diagnosis myocardial ischemia, which is characterized in that be coated on the enzyme electrode
The enzyme that marker reacts can be detected with myocardial ischemia；The nano material combination electrode is by the ink print doped with nano material
It makes；It is coated with nano material on the compound enzyme electrode of nano material and can detect what marker reacted with myocardial ischemia
Enzyme.
A kind of electrode sheet devices of above-mentioned quick diagnosis myocardial ischemia, which is characterized in that it is described can be with myocardial ischemia
The enzyme for detecting marker reaction includes aspartate aminotransferase, and the nano material includes colloidal gold and/or nano-graphite
Alkene.
A kind of electrode sheet devices of above-mentioned quick diagnosis myocardial ischemia, which is characterized in that the myocardial ischemia detection mark
Will object includes IMA and/or Co2+。
The electrode sheet devices of above-mentioned a kind of quick diagnosis myocardial ischemia, which is characterized in that the contact conductor includes setting
The conductive pulp layer of first be placed on substrate and the second conductive pulp layer being coated in the first conductive pulp layer；Described first conductive pulp layer
For silver slurry layer, the described second conductive pulp layer is carbon paste layer or graphene pulp layer；Described includes be set on substrate to electrode
Three conductive pulp layer and the 4th conductive pulp layer being coated in third conduction pulp layer, the third conduction pulp layer is silver slurry layer, described
4th conductive pulp layer is carbon paste layer or graphene pulp layer.
The electrode sheet devices of above-mentioned a kind of quick diagnosis myocardial ischemia, which is characterized in that lid is equipped on the substrate
Body on the lid and is located at signal and generates and be provided with form and gas vent above area.
In addition, the present invention also provides a kind of methods for preparing above-mentioned electrode sheet devices, which is characterized in that including work electricity
The preparation method of pole, the preparation method of the working electrode include that the preparation method of nano material combination electrode or nano material are answered
The preparation method of synthase electrode；
The preparation method of the nano material combination electrode includes: that nano material and ink are prepared by mixing into conductive paste
Obtained electrocondution slurry, is printed on substrate by material using screen printing technique, obtains nano material combination electrode after dry；Institute
Stating the mass ratio of nano material and ink in electrocondution slurry is (0.02~0.2): 1.
The preparation method of the compound enzyme electrode of nano material includes: in substrate surface silver paste for covering layer, in silver paste
Carbon pulp layer is covered on layer, will select enzyme machine drop coating to the surface for the substrate for being covered with carbon pulp layer containing enzyme solutions, dry drop coating, which has, to be contained
The substrate of enzyme solutions obtains the compound enzyme electrode of nano material；The enzyme solutions that contain include colloidal gold composite enzyme solution, nano-graphite
Alkene composite enzyme solution or colloidal gold-nano-graphene composite enzyme solution.
Above-mentioned method, which is characterized in that
The preparation method of the colloidal gold composite enzyme solution includes: to mix colloidal gold with buffer solution, then to mixing
Enzyme is added in liquid, stirs to get colloidal gold composite enzyme solution；The mass ratio of colloidal gold and enzyme in the colloidal gold composite enzyme solution
For 1:(0.01~0.1)；
The preparation method of the nano-graphene composite enzyme solution includes: that enzyme and Tris-HCl solution are mixed to form solution
A is added PEG solution into solution A and forms solution B, nano-graphene is added into solution B, stirs, and ultrasonic disperse is received
Rice graphene composite enzyme solution；Wherein the quality of PEG solution is the 0.5%~2.0% of the quality of solution A；The nano-graphite
The mass ratio of nano-graphene and enzyme is (2~7): (0.1~1) in alkene composite enzyme solution；
The colloidal gold-nano-graphene composite enzyme solution preparation method includes: to mix colloidal gold with buffer solution,
Then enzyme is added into mixed liquor, Tris-HCl solution is added after stirring, forms solution C, PEG solution shape is added into solution C
At solution D, nano-graphene is added into solution D, stirs, it is molten to obtain colloidal gold-nano-graphene complex enzyme for ultrasonic disperse
Liquid；Wherein the quality of PEG solution is the 0.5%~2.0% of solution C quality；The colloidal gold-nano-graphene composite enzyme solution
The mass ratio of middle colloidal gold, enzyme and nano-graphene is 1:(0.01~0.1): (0.2~0.7).
Above-mentioned method, which is characterized in that buffer solution is PBS buffer solution or borax-borate buffer；The PBS is slow
The pH of fliud flushing is 7.0~8.0；The pH of the borax-borate buffer is 8.0~9.6；The pH of Tris-HCl solution be 7.0~
8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Further, the present invention also provides a kind of electrode sheet devices such as above-mentioned quick diagnosis myocardial ischemia to diagnose
Application in myocardial ischemia.
Compared with the prior art, the present invention has the following advantages:
1, the electrode sheet devices of quick diagnosis myocardial ischemia of the invention are based on the enzyme and/or nano material on working electrode
The response signal generated is contacted with tested substance, converts measurable telecommunications for response signal amplification using electrode sheet devices
Number, the signal of myocardial ischemia disease Testing index can be quick and precisely obtained, performance is stablized.
2, the compound enzyme electrode of nano material of the invention, which passes through, will contain enzyme solutions modification to the special of electrode surface formation IMA
Property recognition unit, containing in enzyme solutions added with large specific surface area and more active sites nano material, table can be increased
The enzyme content and reactivity in face effectively improve specificity, sensitivity and the response efficiency of myocardial ischemia disease Indexs measure.
3, nano material combination electrode of the invention is conductive by the way that nano material and ink are prepared by mixing into electrocondution slurry
Slurry is printed on substrate surface and forms working electrode, can effectively improve the absorption to detected material and response efficiency；Meanwhile colloid
Gold and nano-graphene have excellent electric conductivity, and the electric signal that can further enhance working electrode generates and stabilizing power,
The conductive process of signal is free from the influence of the external environment, can effectively extend the service life of electrode slice.
4, the working electrode of multichannel can be set in the electrode sheet devices of quick diagnosis myocardial ischemia of the invention, realizes same
When detect the purposes of a variety of disease indicators.
With reference to the accompanying drawings and examples, technical scheme of the present invention will be described in further detail.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 10.
Fig. 2 is that the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 10 remove the structural representation of lid
Figure.
Fig. 3 is that the structure of the contact conductor of the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 10 is shown
It is intended to.
Fig. 4 is the structural representation to electrode of the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 10
Figure.
Fig. 5 is that the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 11 remove the structural representation of lid
Figure.
Fig. 6 is that the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 14 remove the structural representation of lid
Figure.
Fig. 7 is that the electrode sheet devices of the quick diagnosis myocardial ischemia of the embodiment of the present invention 17 remove the structural representation of lid
Figure.
Description of symbols:
1-substrate；2-signals generate area；3-contact conductors；
The conductive pulp layer of 3-1-second；The conductive pulp layer of 3-2-first；4-working electrodes；
5-to electrode；5-1-third conduction pulp layer；The conductive pulp layer of 5-2-the 4th；
6-reference electrodes；7-lids；8-forms；
9-gas vents.
Specific embodiment
Embodiment 1
The preparation of colloidal gold composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, stirs to get
Colloidal gold composite enzyme solution；The mass ratio of colloidal gold and enzyme is 1:0.1 in the colloidal gold composite enzyme solution；The buffer solution
For PBS buffer solution, the pH of PBS buffer solution is 7.0~8.0.
Embodiment 2
The preparation of colloidal gold composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, stirs to get
Colloidal gold composite enzyme solution；The mass ratio of colloidal gold and enzyme is 1:0.01 in the colloidal gold composite enzyme solution；The buffering is molten
Liquid is PBS buffer solution, and the pH of PBS buffer solution is 7.0~8.0.
Embodiment 3
The preparation of colloidal gold composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, stirs to get
Colloidal gold composite enzyme solution；The mass ratio of colloidal gold and enzyme is 1:0.05 in the colloidal gold composite enzyme solution；The buffering is molten
Liquid is borax-borate buffer, and borax-borate buffer pH is 8.0~9.6.
Embodiment 4
The preparation of nano-graphene composite enzyme solution:
Aspartate aminotransferase and Tris-HCl solution are mixed to form solution A, PEG solution is added into solution A
Solution B is formed, nano-graphene is added into solution B, is stirred, ultrasonic disperse obtains nano-graphene composite enzyme solution；Wherein
The quality of PEG solution is the 0.5% of the quality of solution A；Nano-graphene and enzyme in the nano-graphene composite enzyme solution
Mass ratio is 5:0.1；The pH of Tris-HCl solution is 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 5
The preparation of nano-graphene composite enzyme solution:
Aspartate aminotransferase and Tris-HCl solution are mixed to form solution A, PEG solution is added into solution A
Solution B is formed, nano-graphene is added into solution B, is stirred, ultrasonic disperse obtains nano-graphene composite enzyme solution；Wherein
The quality of PEG solution is the 1.0% of the quality of solution A；Nano-graphene and enzyme in the nano-graphene composite enzyme solution
Mass ratio is 2:1；The pH of Tris-HCl solution is 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 6
The preparation of nano-graphene composite enzyme solution:
Aspartate aminotransferase and Tris-HCl solution are mixed to form solution A, PEG solution is added into solution A
Solution B is formed, nano-graphene is added into solution B, is stirred, ultrasonic disperse obtains nano-graphene composite enzyme solution；Wherein
The quality of PEG solution is the 2.0% of the quality of solution A；Nano-graphene and enzyme in the nano-graphene composite enzyme solution
Mass ratio is 7:0.5；The pH of Tris-HCl solution is 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 7
The preparation of colloidal gold-nano-graphene composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, is added after stirring
Enter Tris-HCl solution, form solution C, PEG solution is added into solution C and forms solution D, nano-graphite is added into solution D
Alkene, stirring, ultrasonic disperse obtain colloidal gold-nano-graphene composite enzyme solution；Wherein the quality of PEG solution is solution C quality
0.5%；The mass ratio of colloidal gold, enzyme and nano-graphene is 1 in the colloidal gold-nano-graphene composite enzyme solution:
0.01:0.5；Wherein, buffer solution is PBS buffer solution, and the pH of PBS buffer solution is 7.0~8.0；The pH of Tris-HCl solution is
7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 8
The preparation of colloidal gold-nano-graphene composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, is added after stirring
Enter Tris-HCl solution, form solution C, PEG solution is added into solution C and forms solution D, nano-graphite is added into solution D
Alkene, stirring, ultrasonic disperse obtain colloidal gold-nano-graphene composite enzyme solution；Wherein the quality of PEG solution is solution C quality
1.0%；The mass ratio of colloidal gold, enzyme and nano-graphene is 1 in the colloidal gold-nano-graphene composite enzyme solution:
0.1:0.2；Wherein, buffer solution is borax-borate buffer, and borax-borate buffer pH is 8.0~9.6；Tris-HCl
The pH of solution is 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 9
The preparation of colloidal gold-nano-graphene composite enzyme solution:
Colloidal gold is mixed with buffer solution, aspartate aminotransferase is then added into mixed liquor, is added after stirring
Enter Tris-HCl solution, form solution C, PEG solution is added into solution C and forms solution D, nano-graphite is added into solution D
Alkene, stirring, ultrasonic disperse obtain colloidal gold-nano-graphene composite enzyme solution；Wherein the quality of PEG solution is solution C quality
2.0%；The mass ratio of colloidal gold, enzyme and nano-graphene is 1 in the colloidal gold-nano-graphene composite enzyme solution:
0.05:0.7；Wherein, buffer solution is borax-borate buffer, and borax-borate buffer pH is 8.0~9.6；Tris-
The pH of HCl solution is 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
Embodiment 1 is into embodiment 9, the process for preparation of PBS buffer solution used are as follows: takes sodium chloride 7.89g, potassium chloride
0.2g, Anhydrous Disodium Phosphate 1.41g, potassium dihydrogen phosphate 0.27g are dissolved with purified water, and salt acid for adjusting pH, purified water is settled to
1000mL；
The process for preparation of borax-borate buffer used are as follows: take borax (molecular formula Na2B4O7·10H2O) 7.8g, boron
Sour 5.2g, is dissolved with purified water, and salt acid for adjusting pH, purified water is settled to 1000mL.
Embodiment 10
As shown in Figure 1, Figure 2, Fig. 3 and Fig. 4, the electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1,
It is provided with signal on the substrate 1 and generates area 2, the signal, which generates, to be provided with to electrode 5, reference electrode 6 and work electricity in area 2
Pole 4, the contact conductor 3 to connect one to one with each electrode is provided on the substrate 1, and the working electrode 4 is nano material
Compound enzyme electrode, the number of the compound enzyme electrode of nano material are 4.
It is coated with nano material on the compound enzyme electrode of nano material and marker can be detected with myocardial ischemia and reacts
Enzyme；It is described to detect the enzyme that marker reacts with myocardial ischemia as aspartate aminotransferase；The nano material packet
Include colloidal gold and/or nano-graphene；The myocardial ischemia detection marker is IMA.
Wherein the compound enzyme electrode of nano material the preparation method comprises the following steps:
Silver paste for covering layer at working electrode 4 is corresponded in substrate surface, carbon pulp layer is covered on silver paste layer, by embodiment
1 selects the institute that covering at working electrode 4 is corresponded in enzyme machine drop coating to substrate to the use containing enzyme solutions prepared by 9 any embodiment of embodiment
It states in carbon pulp layer, there is the substrate containing enzyme solutions to be dried drop coating, obtain the compound enzyme electrode of nano material.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is carbon paste layer；Institute
Stating to electrode 5 includes that the third conduction pulp layer 5-1 being set on substrate 1 and the 4th be coated on third conduction pulp layer 5-1 are led
Plasma-based layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is carbon paste layer.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 11
Such as Fig. 5, the electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are set on the substrate 1
It is equipped with signal and generates area 2, the signal, which generates, to be provided in area 2 to electrode 5, reference electrode 6 and working electrode 4, the substrate 1
On be provided with the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is nano material combination electrode, nanometer
The number of Material cladding electrode is 1, and the nano material combination electrode is printed by the ink doped with nano material.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, obtains nano material combination electrode after dry；The nano material be colloidal gold and nano-graphene, it is described to lead
The mass ratio of nano material and ink is 0.2:1 in plasma-based material, and the mass ratio of colloidal gold and nano-graphene is 1:1；This implementation
In example, the nano material may be colloidal gold or nano-graphene.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is carbon paste layer；Institute
Stating to electrode 5 includes that the third conduction pulp layer 5-1 being set on substrate 1 and the 4th be coated on third conduction pulp layer 5-1 are led
Plasma-based layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is graphene pulp layer；Graphene
Pulp layer is the ink layer doped with graphene.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 12
The electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are provided with letter on the substrate 1
Number generate area 2, the signal, which generates, to be provided with to electrode 5, reference electrode 6 and working electrode 4 in area 2, is arranged on the substrate 1
There is the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is nano material combination electrode, the nanometer material
Material combination electrode is printed by the ink doped with nano material.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, obtains nano material combination electrode after dry；The nano material be colloidal gold and nano-graphene, it is described to lead
The mass ratio of nano material and ink is 0.02:1 in plasma-based material, and the mass ratio of colloidal gold and nano-graphene is 1:1；This implementation
In example, the nano material may be colloidal gold or nano-graphene.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is graphene slurry
Layer；Graphene pulp layer is the ink layer doped with graphene；Described includes the third conductive paste being set on substrate 1 to electrode 5
Layer 5-1 and the 4th conductive pulp layer 5-2 being coated on third conduction pulp layer 5-1, the third conduction pulp layer 5-1 is silver slurry layer,
The 4th conduction pulp layer 5-2 is carbon paste layer.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 13
The electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are provided with letter on the substrate 1
Number generate area 2, the signal, which generates, to be provided with to electrode 5, reference electrode 6 and working electrode 4 in area 2, is arranged on the substrate 1
There is the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is nano material combination electrode, the nanometer material
Material combination electrode is printed by the ink doped with nano material.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, nano material combination electrode is obtained after drying process；The nano material is colloidal gold and nano-graphene, institute
Stating in electrocondution slurry the mass ratio of nano material and ink is 0.1:1, and the mass ratio of colloidal gold and nano-graphene is 3:2；This
In embodiment, the nano material may be colloidal gold or nano-graphene.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is graphene slurry
Layer；The for including the third conduction pulp layer 5-1 that is set on substrate 1 to electrode 5 and being coated on third conduction pulp layer 5-1
Four conductive pulp layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is graphene pulp layer；Stone
Black alkene pulp layer is the ink layer doped with graphene.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 14
Such as Fig. 6, the electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are set on the substrate 1
It is equipped with signal and generates area 2, the signal, which generates, to be provided in area 2 to electrode 5, reference electrode 6 and working electrode 4, the substrate 1
On be provided with the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is nano material combination electrode and nanometer
The number of Material cladding enzyme electrode, nano material combination electrode and the compound enzyme electrode of nano material is respectively 1.
The nano material combination electrode is printed by the ink doped with nano material；The nano material complex enzyme
It is coated with nano material on electrode and the enzyme that marker reacts can be detected with myocardial ischemia；It is described to be detected with myocardial ischemia
The enzyme of marker reaction is aspartate aminotransferase；The nano material includes colloidal gold and/or nano-graphene；It is described
It is IMA that myocardial ischemia, which detects marker,.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, obtains nano material combination electrode after dry；The nano material is nano-graphene, in the electrocondution slurry
The mass ratio of nano-graphene and ink is 0.02:1；In this implementation, the nano material may be colloidal gold, or to receive
Rice graphene and colloidal gold.
The compound enzyme electrode of nano material the preparation method comprises the following steps:
Silver paste for covering layer at working electrode 4 is corresponded in substrate surface, carbon pulp layer is covered on silver paste layer, by embodiment
1 selects the institute that covering at working electrode 4 is corresponded in enzyme machine drop coating to substrate to the use containing enzyme solutions prepared by 9 any embodiment of embodiment
It states in carbon pulp layer, dry drop coating has the substrate containing enzyme solutions, obtains the compound enzyme electrode of nano material.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is carbon paste layer；Institute
Stating to electrode 5 includes that the third conduction pulp layer 5-1 being set on substrate 1 and the 4th be coated on third conduction pulp layer 5-1 are led
Plasma-based layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is carbon paste layer.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 15
The electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are provided with letter on the substrate 1
Number generate area 2, the signal, which generates, to be provided with to electrode 5, reference electrode 6 and working electrode 4 in area 2, is arranged on the substrate 1
There is the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is that nano material combination electrode and nano material are multiple
Synthase electrode.
The nano material combination electrode is printed by the ink doped with nano material；The nano material complex enzyme
It is coated with nano material on electrode and the enzyme that marker reacts can be detected with myocardial ischemia；It is described to be detected with myocardial ischemia
The enzyme of marker reaction is aspartate aminotransferase；The nano material includes colloidal gold and/or nano-graphene；It is described
It is IMA that myocardial ischemia, which detects marker,.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, obtains nano material combination electrode after dry；The nano material is nano-graphene, in the electrocondution slurry
The mass ratio of nano-graphene and ink is 0.1:1；In this implementation, the nano material may be colloidal gold, or to receive
Rice graphene and colloidal gold.
The compound enzyme electrode of nano material the preparation method comprises the following steps:
Silver paste for covering layer at working electrode 4 is corresponded in substrate surface, carbon pulp layer is covered on silver paste layer, by embodiment
1 selects the institute that covering at working electrode 4 is corresponded in enzyme machine drop coating to substrate to the use containing enzyme solutions prepared by 9 any embodiment of embodiment
It states in carbon pulp layer, dry drop coating has the substrate containing enzyme solutions, obtains the compound enzyme electrode of nano material.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is carbon paste layer；Institute
Stating to electrode 5 includes that the third conduction pulp layer 5-1 being set on substrate 1 and the 4th be coated on third conduction pulp layer 5-1 are led
Plasma-based layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is graphene pulp layer；Graphene
Pulp layer is the ink layer doped with graphene.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 16
The electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are provided with letter on the substrate 1
Number generate area 2, the signal, which generates, to be provided with to electrode 5, reference electrode 6 and working electrode 4 in area 2, is arranged on the substrate 1
There is the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is that nano material combination electrode and nano material are multiple
Synthase electrode.
The nano material combination electrode is printed by the ink doped with nano material；The nano material complex enzyme
It is coated with nano material on electrode and the enzyme that marker reacts can be detected with myocardial ischemia；It is described to be detected with myocardial ischemia
The enzyme of marker reaction is aspartate aminotransferase；The nano material includes colloidal gold and/or nano-graphene；It is described
It is IMA that myocardial ischemia, which detects marker,.
Wherein nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, obtains nano material combination electrode after dry；The nano material is nano-graphene, in the electrocondution slurry
The mass ratio of nano-graphene and ink is 0.2:1；In this implementation, the nano material may be colloidal gold, or to receive
Rice graphene and colloidal gold.
The compound enzyme electrode of nano material the preparation method comprises the following steps:
Silver paste for covering layer at working electrode 4 is corresponded in substrate surface, carbon pulp layer is covered on silver paste layer, by embodiment
1 selects the institute that covering at working electrode 4 is corresponded in enzyme machine drop coating to substrate to the use containing enzyme solutions prepared by 9 any embodiment of embodiment
It states in carbon pulp layer, dry drop coating has the substrate containing enzyme solutions, obtains the compound enzyme electrode of nano material.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is graphene slurry
Layer；Graphene pulp layer is the ink layer doped with graphene；Described includes the third conductive paste being set on substrate 1 to electrode 5
Layer 5-1 and the 4th conductive pulp layer 5-2 being coated on third conduction pulp layer 5-1, the third conduction pulp layer 5-1 is silver slurry layer,
The 4th conduction pulp layer 5-2 is carbon paste layer.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
Embodiment 17
Such as Fig. 7, the electrode sheet devices of the quick diagnosis myocardial ischemia of the present embodiment, including substrate 1 are set on the substrate 1
It is equipped with signal and generates area 2, the signal, which generates, to be provided in area 2 to electrode 5, reference electrode 6 and working electrode 4, the substrate 1
On be provided with the contact conductor 3 to connect one to one with each electrode, the working electrode 4 is 3, wherein enzyme electrode, nanometer material
Expect combination electrode and the compound enzyme electrode of nano material each 1.
The enzyme that marker reacts can be detected with myocardial ischemia by being coated on the enzyme electrode；The nano material compound electric
It is printed by the ink doped with nano material pole；Nano material and can is coated on the compound enzyme electrode of nano material
The enzyme reacted with myocardial ischemia detection marker；It is described to detect the enzyme that marker reacts with myocardial ischemia as aspartic acid ammonia
Based transferase；The nano material includes colloidal gold and/or nano-graphene；The myocardial ischemia detection marker is IMA.
Wherein enzyme electrode the preparation method comprises the following steps: silver paste for covering layer at working electrode is corresponded in substrate surface, in silver paste
Carbon pulp layer is covered on layer, and aspartate aminotransferase and buffer solution are mixed into aspartate aminotransferase solution, it will
Aspartate aminotransferase solution is done with selecting in the carbon pulp layer for corresponding in enzyme machine drop coating to substrate and covering at working electrode
Dry drop coating has the substrate of aspartate aminotransferase solution, obtains enzyme electrode.
Nano material combination electrode the preparation method comprises the following steps:
Nano material and ink are prepared by mixing into electrocondution slurry, print obtained electrocondution slurry using screen printing technique
It brushes on substrate, nano material combination electrode is obtained after drying process；The nano material is colloidal gold, in the electrocondution slurry
The mass ratio of colloidal gold and ink is 0.2:1；In the present embodiment, the nano material may be nano-graphene, Huo Zhewei
Colloidal gold and nano-graphene.
The compound enzyme electrode of nano material the preparation method comprises the following steps:
Silver paste for covering layer at working electrode 4 is corresponded in substrate surface, carbon pulp layer is covered on silver paste layer, by embodiment
1 selects the institute that covering at working electrode 4 is corresponded in enzyme machine drop coating to substrate to the use containing enzyme solutions prepared by 9 any embodiment of embodiment
It states in carbon pulp layer, dry drop coating has the substrate containing enzyme solutions, obtains the compound enzyme electrode of nano material.
Contact conductor 3 includes the first conduction pulp layer 3-2 being set on substrate 1 and is coated on the first conductive pulp layer 3-2
The second conductive pulp layer 3-1；The first conduction pulp layer 3-2 is silver slurry layer, and the second conduction pulp layer 3-1 is graphene slurry
Layer；The for including the third conduction pulp layer 5-1 that is set on substrate 1 to electrode 5 and being coated on third conduction pulp layer 5-1
Four conductive pulp layer 5-2, the third conduction pulp layer 5-1 are silver slurry layer, and the 4th conduction pulp layer 5-2 is graphene pulp layer；Stone
Black alkene pulp layer is the ink layer doped with graphene.
In the present embodiment, substrate 1 is PET sheet, it is also possible to the replacement of other substrate materials.
Lid 7 is installed on the substrate 1, on the lid 7 and is located at signal and generates and be provided with 8 He of form above area 2
Gas vent 9.
The testing principle of electrode sheet devices of the invention are as follows: the signal of blood sample to be measured and electrode sheet devices is generated into area and is connect
Touching is observed blood sample to be measured by the form at upper cover and is spread to working electrode；
Working electrode is enzyme electrode, on enzyme electrode IMA in coated aspartate aminotransferase and blood sample to be measured
Biochemical reaction occurs, the amplified multi-channel electrochemical work station that is transmitted to of electric signal that biochemical reaction generates carries out at analysis
Reason, obtain IMA afterwards compared with standard curve contains numerical quantity；
Working electrode is nano material combination electrode, and the nanoparticle of electrode surface contacts production with the IMA in blood sample to be measured
Raw response signal, nano material and electrocondution slurry with high conductance act synergistically, and improve the transfer rate of electronics, telecommunications
Number conduction is analyzed and processed to multichannel electrochemical workstation, obtained afterwards compared with standard curve IMA containing numerical quantity；
Working electrode is the compound enzyme electrode of nano material, and coated aspartic acid amino turns on the compound enzyme electrode of nano material
Biochemical reaction occurs for the IMA moved in enzyme and blood sample to be measured, and the electric signal for reacting generation amplified is transmitted to multi-channel electrochemical
Work station is analyzed and processed, and obtain IMA afterwards compared with standard curve contains numerical quantity；
Blood sample and working electrode contact to be measured, if the content of IMA is more than detection range in blood sample to be measured, electrode is on chip
Set the numerical fluctuations that the electric signal to be formed is unstable, and electric signal exports after conducting to electrochemical workstation.
The above is only presently preferred embodiments of the present invention, not does any restrictions to the present invention, all according to invention skill
Art any simple modification substantially to the above embodiments, change and equivalent structural changes, still fall within the technology of the present invention
In the protection scope of scheme.
Claims (10)
1. a kind of electrode sheet devices of quick diagnosis myocardial ischemia, including substrate (1), which is characterized in that set on the substrate (1)
It is equipped with signal and generates area (2), the signal, which generates, to be provided in area (2) to electrode (5), reference electrode (6) and working electrode
(4), the contact conductor (3) to connect one to one with each electrode is provided on the substrate (1)；The working electrode (4) includes
One or more of enzyme electrode, nano material combination electrode and the compound enzyme electrode of nano material.
2. a kind of electrode sheet devices of quick diagnosis myocardial ischemia according to claim 1, which is characterized in that the enzyme electricity
The enzyme that marker reacts can be detected with myocardial ischemia by being coated on extremely；The nano material combination electrode is by doped with nanometer material
The ink of material is printed；It is coated with nano material on the compound enzyme electrode of nano material and can be detected with myocardial ischemia and marks
The enzyme of will object reaction.
3. a kind of electrode sheet devices of quick diagnosis myocardial ischemia according to claim 2, which is characterized in that it is described can
Include aspartate aminotransferase with the enzyme that react of myocardial ischemia detection marker, the nano material include colloidal gold with/
Or nano-graphene.
4. a kind of electrode sheet devices of quick diagnosis myocardial ischemia according to claim 2 or 3, which is characterized in that described
It includes IMA and/or Co that myocardial ischemia, which detects marker,2+。
5. a kind of electrode sheet devices of quick diagnosis myocardial ischemia according to claim 1, which is characterized in that the electrode
Lead (3) include the first conductive pulp layer (3-2) being set on substrate (1) and be coated in the first conduction pulp layer (3-2) the
Two conductive pulp layer (3-1)；The first conductive pulp layer (3-2) is silver slurry layer, the described second conductive pulp layer (3-1) be carbon paste layer or
Graphene pulp layer；It is described to include the third conduction pulp layer (5-1) that is set on substrate (1) to electrode (5) and be coated on third and lead
The 4th conductive pulp layer (5-2) on plasma-based layer (5-1), the third conduction pulp layer (5-1) is silver slurry layer, the 4th conductive paste
Layer (5-2) is carbon paste layer or graphene pulp layer.
6. a kind of electrode sheet devices of quick diagnosis myocardial ischemia according to claim 1, which is characterized in that the substrate
(1) it is equipped on lid (7), generates on the lid (7) and positioned at signal and be provided with form (8) and gas vent above area (2)
(9)。
7. a kind of method of the electrode sheet devices prepared as described in any claim in claims 1 to 3, which is characterized in that
Preparation method including working electrode, the preparation method of the working electrode include nano material combination electrode preparation method or
The preparation method of the compound enzyme electrode of nano material；
The preparation method of the nano material combination electrode includes: that nano material and ink are prepared by mixing into electrocondution slurry, is answered
Obtained electrocondution slurry is printed on substrate with screen printing technique, obtains nano material combination electrode after dry；It is described to lead
The mass ratio of nano material and ink is (0.02~0.2) in plasma-based material: 1.
The preparation method of the compound enzyme electrode of nano material includes: in substrate surface silver paste for covering layer, on silver paste layer
Carbon pulp layer is covered, enzyme machine drop coating will be selected to the surface for the substrate for being covered with carbon pulp layer containing enzyme solutions, dry drop coating has molten containing enzyme
The substrate of liquid obtains the compound enzyme electrode of nano material；Described containing enzyme solutions includes that colloidal gold composite enzyme solution, nano-graphene are multiple
Synthase solution or colloidal gold-nano-graphene composite enzyme solution.
8. the method according to the description of claim 7 is characterized in that
The preparation method of the colloidal gold composite enzyme solution includes: to mix colloidal gold with buffer solution, then into mixed liquor
Enzyme is added, stirs to get colloidal gold composite enzyme solution；The mass ratio of colloidal gold and enzyme is 1 in the colloidal gold composite enzyme solution:
(0.01~0.1)；
The preparation method of the nano-graphene composite enzyme solution includes: that enzyme and Tris-HCl solution are mixed to form solution A, to
PEG solution is added in solution A and forms solution B, nano-graphene is added into solution B, stirs, ultrasonic disperse obtains a nanometer stone
Black alkene composite enzyme solution；Wherein the quality of PEG solution is the 0.5%~2.0% of the quality of solution A；The nano-graphene is multiple
The mass ratio of nano-graphene and enzyme is (2~7): (0.1~1) in synthase solution；
The colloidal gold-nano-graphene composite enzyme solution preparation method includes: to mix colloidal gold with buffer solution, then
Enzyme is added into mixed liquor, Tris-HCl solution is added after stirring, forms solution C, addition PEG solution is formed molten into solution C
Nano-graphene is added into solution D by liquid D, and stirring, ultrasonic disperse obtains colloidal gold-nano-graphene composite enzyme solution；Its
The quality of middle PEG solution is the 0.5%~2.0% of solution C quality；Glue in the colloidal gold-nano-graphene composite enzyme solution
The mass ratio of body gold, enzyme and nano-graphene is 1:(0.01~0.1): (0.2~0.7).
9. according to the method described in claim 8, it is characterized in that, buffer solution is PBS buffer solution or borax-borate buffer
Liquid；The pH of the PBS buffer solution is 7.0~8.0；The pH of the borax-borate buffer is 8.0~9.6；Tris-HCl solution
PH be 7.0~8.0；The concentration of PEG is 0.1g/mL in PEG solution.
10. a kind of electrode sheet devices answering in diagnosis of myocardial ischemia of quick diagnosis myocardial ischemia as described in claim 1
With.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811423915.8A CN109321431A (en) | 2018-11-27 | 2018-11-27 | A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811423915.8A CN109321431A (en) | 2018-11-27 | 2018-11-27 | A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109321431A true CN109321431A (en) | 2019-02-12 |
Family
ID=65258986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811423915.8A Pending CN109321431A (en) | 2018-11-27 | 2018-11-27 | A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109321431A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114002292A (en) * | 2021-11-02 | 2022-02-01 | 安序源生物科技(深圳)有限公司 | Saliva blood sugar correction device and method |
| CN116087286A (en) * | 2023-03-10 | 2023-05-09 | 上海微创生命科技有限公司 | Biosensor and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004005908A1 (en) * | 2002-07-02 | 2004-01-15 | Matsushita Electric Industrial Co., Ltd. | Biosensor, biosensor chip, and biosensor device |
| EP1486778A2 (en) * | 2003-06-09 | 2004-12-15 | I-Sens, Inc. | Electrochemical biosensor |
| CN102023181A (en) * | 2009-09-21 | 2011-04-20 | 清华大学 | Enzyme electrode and preparation method thereof |
| CN105877694A (en) * | 2014-08-30 | 2016-08-24 | 孙宗正 | Tissue fluid constituent sensor |
| CN209178403U (en) * | 2018-11-27 | 2019-07-30 | 西安良升生物科技有限公司 | A kind of electrode sheet devices for myocardial ischemia quick diagnosis |
-
2018
- 2018-11-27 CN CN201811423915.8A patent/CN109321431A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004005908A1 (en) * | 2002-07-02 | 2004-01-15 | Matsushita Electric Industrial Co., Ltd. | Biosensor, biosensor chip, and biosensor device |
| EP1486778A2 (en) * | 2003-06-09 | 2004-12-15 | I-Sens, Inc. | Electrochemical biosensor |
| CN102023181A (en) * | 2009-09-21 | 2011-04-20 | 清华大学 | Enzyme electrode and preparation method thereof |
| CN105877694A (en) * | 2014-08-30 | 2016-08-24 | 孙宗正 | Tissue fluid constituent sensor |
| CN209178403U (en) * | 2018-11-27 | 2019-07-30 | 西安良升生物科技有限公司 | A kind of electrode sheet devices for myocardial ischemia quick diagnosis |
Non-Patent Citations (2)
| Title |
|---|
| 彭双清等: "《药物安全性评价关键技术》", 30 October 2013, 军事医学科学出版社, pages: 491 * |
| 邓小林等: "《心脏标志物实验室检测应用指南》", 31 July 2015, 中国科学技术出版社, pages: 146 - 147 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114002292A (en) * | 2021-11-02 | 2022-02-01 | 安序源生物科技(深圳)有限公司 | Saliva blood sugar correction device and method |
| CN114002292B (en) * | 2021-11-02 | 2024-03-08 | 深圳清华大学研究院 | Saliva blood glucose correction device and method |
| CN116087286A (en) * | 2023-03-10 | 2023-05-09 | 上海微创生命科技有限公司 | Biosensor and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ji et al. | Smartphone-based integrated voltammetry system for simultaneous detection of ascorbic acid, dopamine, and uric acid with graphene and gold nanoparticles modified screen-printed electrodes | |
| Pan et al. | Preparation of electrochemical sensor based on zinc oxide nanoparticles for simultaneous determination of AA, DA, and UA | |
| Bagheri et al. | Sensitive and simple simultaneous determination of morphine and codeine using a Zn 2 SnO 4 nanoparticle/graphene composite modified electrochemical sensor | |
| CN107153089B (en) | A kind of preparation method of dendroid nano-complex Doxorubicin electrochemical sensor | |
| Wei et al. | Fabrication of CeO2 nanoparticles modified glassy carbon electrode and its application for electrochemical determination of UA and AA simultaneously | |
| WO2021223316A1 (en) | Preparation method for ratio electrochemical uric acid sensor based on zinc-coordinated black phosphorus nanosheet composite and bio-enzyme catalysis | |
| CN111537589B (en) | Method for detecting glucose based on cobalt-based metal organic framework enzyme-free glucose sensor | |
| CN105067685B (en) | A kind of blood multi-analyte immunoassay test paper and preparation method thereof | |
| WO2016140543A1 (en) | Enzyme-based potentiometric glucose detection sensor and method for manufacturing same | |
| CN101021529A (en) | High-flux detection system of multianalyte simultaneous detection and electrochemical immunoanalytical method | |
| CN108548854B (en) | Sialic acid electrochemical test paper and preparation and detection method thereof | |
| CN105842321B (en) | The preparation method of the non-enzymatic glucose electrochemical sensor of cupric oxide nano pin/nitrogen-doped graphene composite | |
| CN103604849A (en) | Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid | |
| CN108872343A (en) | A kind of Dopamine Sensor and its preparation and application based on nitrogen-doped graphene | |
| CN109321431A (en) | A kind of electrode sheet devices of quick diagnosis myocardial ischemia and its preparation method and application | |
| Li et al. | Simultaneous detection of lactate and glucose by integrated printed circuit board based array sensing chip | |
| Lou et al. | A 3D bio-platform constructed by glucose oxidase adsorbed on Au nanoparticles assembled polyaniline nanowires to sensitively detect glucose by electrochemiluminescence | |
| CN104597093A (en) | Modified electrode for glucose biosensor as well as preparation method and application thereof | |
| Pal et al. | The detection of mercury, cadium, and arsenic by the deactivation of urease on rhodinized carbon | |
| CN108802390A (en) | A kind of preparation of the pancreatic tumour marker immunosensor based on graphene-gold-palladium nanocomposite | |
| CN1912626A (en) | Preparation of disposable carbon-base glucose chip and electrochemical detection method | |
| Sołoducho et al. | Electrochemical and optical biosensors in medical applications | |
| CN101393158B (en) | Biology sensing enzyme detection electrode for hydrogen peroxide and detection method thereof | |
| CN209178403U (en) | A kind of electrode sheet devices for myocardial ischemia quick diagnosis | |
| CN1908669B (en) | Hemoglobin chip preparation and electrochemical detecting method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |