CN109317514A - Penicillium bacterial strain CN35 is in the application administered in heavy metal pollution - Google Patents
Penicillium bacterial strain CN35 is in the application administered in heavy metal pollution Download PDFInfo
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- CN109317514A CN109317514A CN201811477992.1A CN201811477992A CN109317514A CN 109317514 A CN109317514 A CN 109317514A CN 201811477992 A CN201811477992 A CN 201811477992A CN 109317514 A CN109317514 A CN 109317514A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/206—Manganese or manganese compounds
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Abstract
The invention discloses a kind of penicillium bacterial strain CN35 in the application administered in heavy metal pollution, and penicillium bacterial strain CN35 center quality is loose cotton-shaped or cord-like, and colony edge is low, neat, sporulation quantity is big, spore green, edge white mycelium, Later growth bacterium colony green are deepened.A kind of penicillium bacterial strain CN35 provided by the invention can be used for administering the heavy metal pollutions such as Cd, Pb, Zn, Cu in water body and soil, and will not cause secondary pollution to environment in the application administered in heavy metal pollution.
Description
Technical field
The present invention relates to biotic environment Prevention Technique field, more particularly to penicillium bacterial strain CN35 is administering a huge sum of money
Belong to the application in pollution.
Background technique
Cadmium and zinc are congeners, in nature cadmium often with zinc, lead symbiosis；After environment is by cadmium pollution, cadmium can be
Enrichment, enters human body by food chain and causes slow poisoning in organism.Zinc to the toxicity of fish and aquatic animal compare people and
Warm-blooded animal is many times larger；Zinc is concentrated in the soil, and can make also to be enriched in plant and lead to the people for eating this plant and move
Object is aggrieved；It is affected with rural sewage disposal farm containing zinc to crops especially wheat, will cause wheat uneven emergence, tiller
Few, plant is short and small, blade is sallow；Excessive zinc can also make soil lose activity, and number of bacteria is reduced, and the microorganism in soil makees
With decrease.The exploitation and smelting of copper zinc ore, intermetallic composite coating, machine-building, steel production etc. can all cause copper to atmosphere, water body and
The pollution of soil.The main source of lead contamination has: exploitation and smelting, battery processing containing lead ore, electron wastes return
Receive dismantling and vehicle exhaust etc..
The heavy metals such as Cd, Pb, Zn, Cu can enter human body by food chain, cause to be poisoned；Heavy metal is administered at present
The method multiplicity of pollution, but all exist and administer the problems such as being not thorough or cause new pollution.Therefore it provides one kind will not be to environment
The penicillium bacterial strain CN35 for causing secondary pollution, the problem of for administering heavy metal pollution, being those skilled in the art's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides the penicillium bacterial strain CN35 that one kind will not cause secondary pollution to environment, for controlling
Manage heavy metal pollution.
To achieve the goals above, the present invention adopts the following technical scheme:
Penicillium bacterial strain CN35 is in the application administered in heavy metal pollution.
Further, penicillium bacterial strain CN35 center quality is loose cotton-shaped or cord-like, and colony edge is low, neat,
Sporulation quantity is big, spore green, edge white mycelium, and Later growth bacterium colony green is deepened.
Further, the heavy metal is Cd, Pb, Zn, Cu.
Further, described Cd, Pb, Zn, Cu to the minimum inhibitory concentration of penicillium bacterial strain CN35 be respectively 46, > 80, > 80,
20mM。
Further, penicillium bacterial strain CN35 is to Cd2+The response external manifestation of stress are as follows: colony diameter is with Cd2+Concentration increase and
Reduce；Cd2+When concentration >=2mM, bacterium colony bacterium colony on the CYA plate for do not add Cu, Zn from yellow becomes red, Cd2+Concentration >=
When 5mM, bacterium colony becomes red from green on PDA plate；With Cd2+Concentration increases, and bacterium colony red is deepened on each plate；Bacterium colony
Form is also with Cd2+Concentration changes and changes: Cd2+When concentration is 20mM, bacterium colony surface forms protrusion.
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of penicillium bacterial strains
It is heavy metal-polluted to can be used for administering Cd, Pb, Zn, Cu in water body and soil etc. in the application administered in heavy metal pollution by CN35
Dye, and secondary pollution will not be caused to environment.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawing is schematic diagram of the bacterium colony of CN35 of the present invention on CYA, MEA, OA plate；
In figure: a.CN35 morphological feature: it is CYA reverse side, MEA anti-that upper row, which is respectively CYA, MEA, OA, lower row, from left to right
Face, YES；B. conidiophore and conidium；C. septate hypha；
Fig. 2 attached drawing is the ITS sequence schematic diagram of bacterial strain CN35 of the present invention；
Fig. 3 attached drawing is the BenA gene order phylogenetic tree schematic diagram of bacterial strain CN35 of the present invention；
Fig. 4 attached drawing is penicillium bacterial strain CN35 difference mycelia additive amount of the present invention and Cd2+The adsorption rate of different initial concentrations；
Fig. 5 attached drawing is influence of the different heavy metal ion of the present invention to penicillium bacterial strain CN35 strain growth.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The separation of 1 penicillium bacterial strain CN35 of embodiment is identified
Pedotheque picks up from the Songbo Town, Changning City, Hunan Province village Xin Tong Xiangjiang River basin rice field, sampled point GIS map tool
(Garmin) one season of positioning acquisition late rice 5-20cm soil 2kg is sub-packed in two sterile sealing bags after mixing, one bag is placed in ice
The preservation of 4 DEG C of laboratory is taken back in box, one bag air-dries measurement heavy metal content in soil and pH etc..The soil 10mg of 4 DEG C of preservations is dissolved in
In 90mL aseptic deionized water, magnetic agitation 30min prepares Soil Slurry.Soil Slurry 1mL is taken to be inoculated in Cd containing 2mM
(NO3)2PD fluid nutrient medium in, and streptomysin (30mg/L) is added and inhibits bacterial growth, 150rpm in 28 DEG C of shaken cultivation casees
Under the conditions of resistance to cadmium fungi enrichment culture 7d.Resistance to 100 μ L of cadmium fungi enrichment culture dilution is taken to be coated on 2mM Cd2+On PDA plate,
Well-grown bacterium colony is successively transferred in higher concentration (4,8,16mM) Cd after 28 DEG C of culture 7d2+On PDA plate, gradient domestication training
It supports, further screens, separates, purifying resistance to cadmium fungi；The strongest bacterial strain of cadmium patience is obtained as the strains tested further studied.
Soil sample collection point coordinate: 112 ° 36 ' 08.73 of 26 ° 34 ' 36.27 of N ", E ".Soil total Cd, Cu, Zn, Pb difference
For 17.73,32.42,103.9,58.2mg/kg, pH 6.1.It is true using the resistance to cadmium of concentration of cadmium ions gradient method for domesticating step-sizing
Bacterium, from 2mM Cd2+10 plants of fungi are screened on plate；Continue the Cd for being forwarded to 4mM, 8mM isoconcentration2+On plate, wherein 6 plants
Patience gradually weakens, and in addition still shows stronger cadmium patience for 3 plants；Until switching is in 16mM Cd2+On plate, wherein 3 plants are not given birth to
It is long, only it is left 1 plant and remains to grow；Wherein as the strains tested further studied, number is the strongest bacterial strain of cadmium patience for selection
CN35。
The morphological analysis of resistance to cadmium fungi CN35:
CN35 is inoculated on CYA, MEA, OA, YES, CYAS culture medium flat plate (90mm culture using 3 inocalation methods
Ware), 25 DEG C of dark culturing 7d；Colony diameter is measured after 7d, observation produces spore situation, and front and back sides colony colour and soluble pigment have
Nothing；The bacterium colony material that 7d is cultivated on MEA plate is used for microexamination, observes ascocarp, ascus, ascus spore with OA plate material
Son；Electronic scanner microscope is used in mycelia, conidiophore, pycnidia observation.
Colony characteristics: it is loose cotton-shaped or rope that the bacterium colony of CN35 is especially center quality on CYA, MEA, OA plate
Shape, colony edge is low, clear, neat, and sporulation quantity is big, spore green, edge white mycelium, and Later growth bacterium colony green is deepened
(see Fig. 1 a), and surface has red drop to ooze out；For the back side CYA white to rufous, the back side MEA and OA (not showing in picture) is grey
Green is to pale red；YES: it is slightly prominent in the middle part of bacterium colony, there is crackle；Edge is low, smooth, edge 3-4mm wide；White mycelium；Bacterium colony
Frizzle shape；The sparse formation of spore finally generates a large amount of celadon conidiums；No soluble pigment generates, and bacterium colony reverse side (does not mention
For picture) lark is to olive brown.CN35 can be grown on CYAS, have salt resistance ability.
Microcosmic morphological feature is shown in Fig. 1 b: conidiophore apex has the branch wheel of broom shape, and main two is verticillate, and phial drapes over one's shoulders
Aciculiform, then stigma top generate be in chain spheric conidium, 3 μm of spore diameter or so；Mycelia has diaphragm (Fig. 1 c)；
The morphological feature of these Morphological Features and Penicillium bacterium is coincide；Ascocarp is not observed on the OA culture medium of culture 14d.
ITS, BenA gene order PCR amplification and sequencing of bacterial strain CN35:
Sterilizing toothpick picking hypha,hyphae 100mg is extracted referring to specification with Omega fungal DNA Mini Kit
Separate fungal DNA；ITS sequence is most popular fungal marker sequence, and β-tubulin gene is that fungi second identifies label
Be preferably selected.With ITS universal primer ITS-1 (5 '-TCCTCCGTAGGTGAACCTGCGG-3 ') and ITS-4 (5 '-
TCCTCCGCTTATTGATATGC-3 ') and β-tubulin (BenA) Bt2a- (5 '-GGTAACCAAATCGGTGCTGCTTTC-
3 ') and Bt2b (5 '-ACCCTCAGTGTAGTGACCCTTGGC-3 ') extension increasing sequence.PCR reaction condition are as follows: 95 DEG C of initial denaturations
5min；35 circulations: 94 DEG C of denaturation 45s, 55 DEG C of annealing 45s, 72 DEG C of extension 1min, 72 DEG C, 7min, 10 DEG C of terminations；PCR product
Sequence is measured by Sangon Biotech (Shanghai) Co., Ltd..Blast in ncbi database is logged in, ITS and BenA is compared
Gene order, and therefrom obtain reference sequences and carry out phylogenetic analysis.Phylogenetic analysis is in Mega5.0 software
Neighbor-joining (NJ) method.
BLAST compare analysis the result shows that, ITS sequence, BenA gene order and the Penicillium (Penicillium of CN35
Sp.) and the homology of its epigamous category Talaromyces (Talaromyces sp.) is up to 99%, with 5.0 software building of MEGA
ITS sequence, BenA gene order phylogenetic tree (see Fig. 2,3) confirm the phyletic evolution of CN35 Yu Penicillium and Talaromyces
Relationship is very close；Combining form observation and molecular biological analysis are as a result, identification CN35 belongs to Penicillium (Penicillium
sp.)。
The activation culture of 2 penicillium bacterial strain CN35 of embodiment
The penicillium bacterial strain CN35 for taking laboratory to save, carries out activation culture, the mould of acquisition on CYA, PDA plate respectively
Bacterial strain CN35 center quality is loose cotton-shaped or cord-like, and colony edge is low, neat, and sporulation quantity is big, spore green, edge bacterium
Silk white, Later growth bacterium colony green are deepened.
Adsorption capacity of the 3 penicillium bacterial strain CN35 of embodiment to Cd
The mycothallus of the above-mentioned activation culture 7d on PDA plate is scraped in PD fluid nutrient medium, 150rpm, 28 DEG C of trainings
7d is supported, 8000rpm is centrifuged 15min, aseptically weighs 0.1g, 0.5g, 1g, 1.5g respectively and (1g wet mycelium is taken to be placed in
Dried in 60 DEG C of baking ovens, until mycelia constant mass is constant, calculate wet mycelium dry weight) mycelium is inoculated in 100mg/L respectively
With the Cd of 200mg/L2+In PD fluid nutrient medium, to avoid absorption of the culture medium to cadmium ion, respectively with 100mg/L, 200mg/L
Mycelial PD fluid nutrient medium is not added as control, 150rpm, 28 DEG C of culture 7d, 8000rpm centrifugation 15min use Atomic absorption
Spectrophotometry measures Cd in supernatant2+Concentration；Bacterial strain cadmium adsorption rate is calculated with following formula；All tests are all provided with to be weighed three times
Multiple, results are averaged analyzes for data；
Calculation formula: cadmium adsorption rate Ar (100%)=(Cc-Ct)/Cc × 100%；In formula, Cc is control supernatant
Cd2+Final concentration (mg/L)；Ct is Cd in supernatant after mycelia absorption2+Concentration (mg/L).
As a result, it has been found that being not added with mycelia, Cd2+Initial concentration is Cd in the culture medium of 100mg/L2+Concentration is maintained at
95.77 ± 2.6mg/L, 200mg/L's is maintained at 196.87 ± 5.11mg/L, shows that culture medium does not have the absorption of cadmium ion substantially
Have an impact；It is computed, as a result as shown in figure 4, initial concentration is 200mg/L, addition 1.5g wet mycelium (measures humidified mycelia
When water content is 83%), cadmium adsorption rate highest, and the adsorption rate otherness with addition 0.5g and 1.0g under same initial concentration
It is not significant；And Cd2+When initial concentration is 200mg/L, mycelia 0.5g is added, cadmium adsorption rate when addition 0.1g than significantly improving；When
Cd2+One timing of initial concentration, penicillium bacterial strain CN35 increases the adsorption rate of cadmium with the increase of mycelium additive amount, but continues to increase
Cadmium adsorption rate increasing degree is not significant when adding hyphae length.
Resistance test of the 4 penicillium bacterial strain CN35 of embodiment to heavy metal
The penicillium bacterial strain CN35 of the above-mentioned activation culture on PDA plate is taken successively to be inoculated in 20,25,30,35,40,45mM
Equal Cd2+On the PDA plate of concentration, 28 DEG C of culture 7d, until bacterium colony is not grown, Cd at this time2+Concentration is Cd to penicillium bacterial strain
The MIC value of CN35；Bacterium dish is got on the PDA plate with penicillium bacterial strain CN35 single colonie with diameter 5mm punch, is connect respectively
Kind is in Pb (NO3)2Concentration is respectively 0,20,40,60,80mM, ZnSO4Concentration is respectively 0,20,40,60,80mM, CuSO4Concentration
Respectively 0,2.5,5,10, on the PDA plate of 20mM, 28 DEG C of culture 7d, with ruler crossing method measurement colony diameter (under
Together), colony diameter size can reflect the biomass size that fungi grows on plate.All tests are all provided with to be repeated three times.
As a result, it has been found that penicillium bacterial strain CN35 is successively inoculated on 20-46mM PDA plate, with Cd2+Concentration increase and can be
45mM Cd2+It is grown on PDA plate, when 46mM cannot grow；As shown in Figure 5, penicillium bacterial strain CN35 colony diameter is with each heavy metal
Concentration raising reduces, and illustrates that each heavy metal has inhibiting effect to bacterial strain CN35 growth；In Cu2+It is sterile when concentration is 20mM
It falls and grows, Pb2+、Zn2+When for 80mM, bacterium colony can be grown, but by obvious inhibiting effect, and Pb2+It is smaller to its inhibiting effect
In Zn2+；Cu, Zn, Pb are respectively 20, > 80, > 80 to the MIC value of CN35, i.e., Cd, Cu, Zn, Pb are to penicillium bacterial strain CN35's
MIC value be much higher than soil environment quality secondary standard concentration (pH≤6.5, mg/kg, Pb:250, Zn:200, Cu:50, Cd:
0.3), illustrate that penicillium bacterial strain CN35 has patience to heavy metal Cd, Cu, Zn, Pb；MIC value indicates penicillium bacterial strain CN35 to a huge sum of money
The tolerant levels of category, MIC value is directly proportional to patience, so penicillium bacterial strain CN35 is to the strong and weak sequence of the patience of above several heavy metal species
Are as follows: Pb2+> Zn2+> Cd2+> Cu2+。
5 penicillium bacterial strain CN35 of embodiment is to various concentration Cd2+The response of stress
Take respectively CYA, on PDA plate activation culture penicillium bacterial strain CN35, be inoculated in 0,0.25,0.5,1,2,5,10,
14, the Cd of 20,40mM2+On PDA plate and CYA (to avoid other heavy metal ion to the interference of result, is not added with culture medium institute
Need ingredient-mark secondary element Zn, Cu) on plate, respectively repeat three times, 28 DEG C of continuous culture 7d measurement colony diameters simultaneously observe bacterium colony production
Spore situation, color, colouring matter secretion situation difference.
As a result, it has been found that penicillium bacterial strain CN35 colony colour on two kinds of culture mediums is different, but Cd2+Promote red pigments
Secretion；PDA: control bacterium colony surface is covered with light green color conidium, and center spore color is slightly deep；Cd2+When concentration < 5mM, bacterium
Green constantly intensification, colony edge white hypha is fallen to extend outward；With Cd2+Concentration increases, and bacterium colony conidium is reduced, 5mM
When there was only sparse spore there are bacterium colony center surface, bacterium colony front is red；Bacterium colony reverse side: control yellow green, center is slightly
Red, with Cd2+Concentration increases, and red color range is constantly expanded outward by center, and color is constantly deepened, and white edge part is continuous
Reduce；When red colonies switch back to no cadmium plate again, bacterium colony well-grown, surface is neat, restores green.CYA culture medium is just
Face, control bacterium colony are yellow green, and colony colour is orange red, pale red on 0.5-4mM culture medium, and color is compared with 2mM when 4mM
It is deep；Bacterium colony back side red is deeper, and red pigments are with Cd2+Concentration increases and increases；When red colonies switch back to no cadmium plate again,
Bacterium colony well-grown, surface is neat, and yellow green is restored in front.Colonial morphology is also with Cd2+Concentration changes, bacterium colony and culture medium
In conjunction with increasingly closer；As Cd in PDA2+When concentration 10mM, bacterium colony is smooth；When 20mM or so, when bacterium colony surface is by low concentration
Smooth to become umbilical protrusion, the back side is radial to split.
Penicillium bacterial strain CN35 is very fast in PDA cultured on solid medium, and colony diameter is about 22mm after 28 DEG C of culture 3d,
12d covers with the plate that diameter is 9cm；Different Cd2+On its diameter influence differ greatly, in inhibition concentration between 10-20mM, and
Cd2+When up to 40mM, bacterium colony remains to preferably grow, and shows that penicillium bacterial strain CN35 has stronger resistance to cadmium characteristic.
Influence of the 6 penicillium bacterial strain CN35 of embodiment to Security of rice
Microbe application is it to paddy growth safety in the premise that paddy field carries out cadmium pollution improvement, is not induced an illness,
Do not inhibit to grow.Seedling is sensitive to environment-stress, thus carry out CN35 to rice cause a disease situation and to emergence rate, young root, seedling it is raw
It is long to influence research, it is handled by A group design experiment；Design treatment group B probes into whether this resistance to cadmium fungi has Cd stress rice simultaneously
There is detoxication.Planting soil is the water paddy soil (Cd content is 0.28mg/kg) for picking up from academy of agricultural sciences of Hunan Province, 200mL beaker
In respectively be added air-dry soil 200mg.Applying penicillium bacterial strain CN35 fermentation liquid prior to seeding, (fermentation liquid system penicillium bacterial strain CN35 exists
150rpm, 30 DEG C of shaken cultivation 7d in PD fluid nutrient medium) and stir, 10 buddings of every glass of addition are neat, and (bud is long
Be grain it is long 2/3) rice seed, rice seed first uses 30%H2O2Sterilize 10min, and sterile deionized water soaks seed after cleaning 5 times, is broken
Chest, vernalization processing.3 repetitions of each processing.A group is handled: penicillium bacterial strain CN35 fermentation liquid 0,20,40,60,80mL, at B group
Reason: every glass of addition CdCl2Solution makes soil Cd2+Concentration is 200mg/kg, penicillium bacterial strain CN35 fermentation liquid 0,20,40,60,
80mL, CdCl2Solution is first added in fermentation liquid, and each aseptic deionized water to total volume that supplements is 120mL, is poured into after mixing well
It is stirred evenly in beaker.(32 DEG C) night in 12h daytime (26 DEG C) alternate culture counts emergence rate after 2d, and measurement rice seedlings are long after 8d, seedling
Fresh weight, root fresh weight.Calculate average seedling length and root long.
The rice of addition penicillium bacterial strain CN35 fermentation liquor treatment does not occur symptom during the growth process, and rice seedling rate is not
It is impacted；As shown in table 1, it is not compareed by cadmium pollution group and is with the emergence rate of addition penicillium bacterial strain CN35 fermentation liquor treatment
100%, penicillium bacterial strain CN35 does not influence emergence rate.20-40mL fermentation liquid is added, seedling length is increased slightly, but not with contrast difference
Significantly, when additive amount reaches 60L-80m, seedling is long to be significantly reduced, and high dose 80mL processing result and 60mL without significant difference.Add
Add 20-60mL fermentation liquid, seedling fresh weight is dramatically increased compared with control, and 80mL is handled and compareed difference with insignificance；Root fresh weight is also with hair
The variation of zymotic fluid additive capacity: when 20mL is added, root fresh weight is handled with no significant difference, 40-80mL is compareed, every increase 20mL,
Root fresh weight dramatically increases, and 80mL processing relatively control root fresh weight increases nearly 1 times.As addition Cd2+When 200mg/kg, rice is by bright
It is aobvious to inhibit, but after addition fermentation liquid, paddy growth situation changes: 20-80mL processing dramatically increases seedling length and seedling fresh weight,
60mL and the above dosage increase effect and become apparent from, but root fresh weight just significantly improves when only addition 20mL, as dosage increases,
Rice root fresh weight significantly reduces, and lower than control.The result shows that fermentation liquid 40mL/200g soil treatment dose effect is best.
Table 1
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (5)
1. penicillium bacterial strain CN35 is in the application administered in heavy metal pollution.
2. penicillium bacterial strain CN35 according to claim 1 is in the application administered in heavy metal pollution, which is characterized in that described
Penicillium bacterial strain CN35 center quality is loose cotton-shaped or cord-like, and colony edge is low, neat, and sporulation quantity is big, spore green, side
Edge white mycelium, Later growth bacterium colony green are deepened.
3. penicillium bacterial strain CN35 according to claim 1 is in the application administered in heavy metal pollution, which is characterized in that described
Heavy metal is Cd, Pb, Zn, Cu.
4. penicillium bacterial strain CN35 according to claim 3 is in the application administered in heavy metal pollution, which is characterized in that described
Cd, Pb, Zn, Cu are respectively 46, > 80, > 80,20mM to the minimum inhibitory concentration of penicillium bacterial strain CN35.
5. penicillium bacterial strain CN35 according to claim 5 is in the application administered in heavy metal pollution, which is characterized in that mould
Bacterial strain CN35 is to Cd2+The response external manifestation of stress are as follows: colony diameter is with Cd2+Concentration increases and reduces；Cd2+Concentration >=2mM
When, bacterium colony bacterium colony on the CYA plate for do not add Cu, Zn from yellow becomes red, Cd2+When concentration >=5mM, bacterium colony is flat in PDA
Become red from green on plate；With Cd2+Concentration increases, and bacterium colony red is deepened on each plate；Colonial morphology is also with Cd2+Concentration
Change and change: Cd2+When concentration is 20mM, bacterium colony surface forms protrusion.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468586A (en) * | 2013-08-29 | 2013-12-25 | 北京环氧环保科技发展有限公司 | Penicillium strain and application in treatment of sewage and odor pollution thereof |
| CN104805021A (en) * | 2015-05-15 | 2015-07-29 | 湖南省农业生物技术研究中心 | Cadmium-tolerant penicillium and separation method thereof |
| CN106244471A (en) * | 2016-10-08 | 2016-12-21 | 南京工业大学 | Heavy metal mercury-resistant fungus strain and application thereof |
| CN106434374A (en) * | 2016-09-30 | 2017-02-22 | 湖南省农业生物技术研究中心 | Trichoderma asperellum and application thereof in remediation for heavy metal pollution |
-
2018
- 2018-12-05 CN CN201811477992.1A patent/CN109317514A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468586A (en) * | 2013-08-29 | 2013-12-25 | 北京环氧环保科技发展有限公司 | Penicillium strain and application in treatment of sewage and odor pollution thereof |
| CN104805021A (en) * | 2015-05-15 | 2015-07-29 | 湖南省农业生物技术研究中心 | Cadmium-tolerant penicillium and separation method thereof |
| CN106434374A (en) * | 2016-09-30 | 2017-02-22 | 湖南省农业生物技术研究中心 | Trichoderma asperellum and application thereof in remediation for heavy metal pollution |
| CN106244471A (en) * | 2016-10-08 | 2016-12-21 | 南京工业大学 | Heavy metal mercury-resistant fungus strain and application thereof |
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