CN109315438A - A kind of prevention and treatment biological agent of plant leaf mold and preparation method thereof - Google Patents
A kind of prevention and treatment biological agent of plant leaf mold and preparation method thereof Download PDFInfo
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- CN109315438A CN109315438A CN201811351969.8A CN201811351969A CN109315438A CN 109315438 A CN109315438 A CN 109315438A CN 201811351969 A CN201811351969 A CN 201811351969A CN 109315438 A CN109315438 A CN 109315438A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
Abstract
The invention discloses prevention and treatment biological agent of a kind of plant leaf mold and preparation method thereof, the raw material including following parts by weight: 48-72 parts of microbial cells, 20-40 parts of bacteriostatic agent, 25-45 parts of traditional Chinese medicinal components, 60-80 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: 12-16 parts of mustard seed enzyme preparation, 10-18 parts of colloid bacillus cereus, 15-23 parts of nitrogen-fixing bacteria, 11-15 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include the raw material of following parts by weight: 4-8 parts of chinaberry, 4-12 parts of alumen, plant are powder 15-19 parts viscous.Biological agent prepared by the present invention saves cost, reduces the usage amount of pesticide, reduces pesticide and endangers caused by human health, can be effectively prevented and treat plant leaf mold, while ensure that the quality and yield of plant.
Description
Technical field
The present invention relates to biological agent technical field, the prevention and treatment biological agent of specially a kind of plant leaf mold and its preparation
Method.
Background technique
Leaf mold is also known as red spot disease, and leaf muld of tomato is that greenhouse tomato generally occurs, endangers a kind of serious disease, usually
It causes that blade is withered and yellow and uproots plants after their edible portions have been harvested ahead of time, leaf mold belongs to Deuteromycotina fungi, and fungi is named as yellow branch spore bacterium, Fulvia fulva Ciferri
To be attached to the surface of the seed and the intracutaneous mycelia of adventicous species and remaining and the mycelia being attached in invalid body, frame material and soil and spore
Son is overwintering.Spring in next year under suitable temperature and humidity conditions, generates new conidium, spore is with the wind or the positions such as blade are arrived in ejection
Intrusion, continues up to autumn Mo.The disease epidemic rate is very fast, under the suitable conditions, only needs 10- to full incidence period from the phase is originated
15 days or so time, although and existing common pesticide have the characteristics that it is efficient, in the process of long-time service, disease
Insect pest is also easy to produce the problem of drug resistance, and pesticide very harmful pesticide residue easy to form, makes to atmosphere, water quality, soil
At serious pollution, cause significant damage to human health.
The patent application of Publication No. CN103621580A discloses a kind of manufacturer of the disease-resistant drug of leaf muld of tomato
Method, the component including following unit of weight: 41% 10-15 parts of poly- astatine pyrimethanil, 50% 8-12 parts of Bravo, 6% Fluoxastrobin 10-
15 parts, 4-8 parts of oxazole, 4-8 parts of chlorogenic acid, polyoxy is 6-10 parts clear, and 4-8 parts of the root of Dahurain angelica, 6-10 parts of arrowhead.Manufacturer of the present invention
Method is simple, and product proportion obtained is simple, easy to use, but the antiradiation drug matter of invention preparation, acts on the plants such as tomato
On, effect of drugs is slower.
The patent application of Publication No. CN108076990A, the invention discloses a kind of control method of leaf muld of tomato,
It comprises the following steps that step S1, applies 4000 kilograms of farmyard manure for every 667 square metres before plantation；Step S2, by 8 centimetres of spacing in the rows, greatly
6 centimetres of 20 centimetres of line-spacing, small line-spacing plant tomatoes；Step S3, every 667 square metres of seedling stage are spread fertilizer over the fields plant ash double centner；Step
S4, early spring intertill weeding 3-4 times that preserves soil moisture of loosening the soil；Step S5 grasps tomato growth stage soil moisture content, keeps maxmun field capacity
60%-70%；Step S6, in tomato growth early period, regular foliage-spray wither-resistant agent, phytophthora be clear or potassium dihydrogen phosphate；Step
S7 is sprayed clear water sooner or later, or sprays the sodium hydrogensulfite of 150 mg/kgs；Step S8, tomato is by leaf spot, anthracnose, aphid
Worm, red spider harm after can cause its generate leaf mold, spray medical fluid prevention and treatment.The present invention can significantly prevent and treat tomato leaf
Mildew, and it is safe and harmless to tomato products and plant strain growth.But agriculture of invention during tomato planting to tomato sprinkling
Medicine, is used for a long time, and tomato plant is also easy to produce drug resistance.
Summary of the invention
The purpose of the present invention is to provide prevention and treatment biological agents of a kind of plant leaf mold and preparation method thereof, have solved
State the problem of proposing in background technique.
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 48-72 parts of microbial cells,
20-40 parts of bacteriostatic agent, 25-45 parts of traditional Chinese medicinal components, 60-80 parts of solvent；Wherein microbial cells include the raw material of following parts by weight:
12-16 parts of mustard seed enzyme preparation, 10-18 parts of colloid bacillus cereus, 15-23 parts of nitrogen-fixing bacteria, 11-15 parts of bacillus subtilis；Wherein
Traditional Chinese medicinal components include the raw material of following parts by weight: 4-8 parts of chinaberry, 4-12 parts of alumen, plant are powder 15-19 parts viscous.
Preferably, the raw material including following parts by weight: 54-66 parts of microbial cells, 25-35 parts of bacteriostatic agent, traditional Chinese medicinal components
30-40 parts, 65-75 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: 13-15 parts of mustard seed enzyme preparation, colloid
12-16 parts of bacillus, 17-21 parts of nitrogen-fixing bacteria, 12-14 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: viscous powder 16-18 parts of 5-7 parts of chinaberry, 6-10 parts of alumen, plant.
Preferably, the raw material including following parts by weight: 60 parts of microbial cells, 30 parts of bacteriostatic agent, 35 parts of traditional Chinese medicinal components, molten
70 parts of agent；Wherein microbial cells include the raw material of following parts by weight: 14 parts of mustard seed enzyme preparation, 14 parts of colloid bacillus cereus, Gu
19 parts of nitrogen bacterium, 13 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include the raw material of following parts by weight: 6 parts of chinaberry, 8 parts of alumen,
Plant glues 17 parts of powder.
Preferably, the bacteriostatic agent includes the raw material of following parts by weight: 2-6 parts of Huang Shi purified powder, horseradish extract 3-11
Part, 10-18 parts of poly- astatine pyrimethanil, dish fruit strengthen 0.2-0.6 parts of base of a fruit spirit.
Preferably, the solvent is ethyl alcohol；The plant glues the raw material that powder includes following mass percent: water hemp skin powder
41-45%, elm bark powder are 55-59%.
Preferably, the mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture
To be put into vibration culture 2-4 days in the flask that fluid nutrient medium is housed, cultivation temperature is 25-29 DEG C, obtains liquid pure culture,
Liquid pure culture is crossed into gauze, the mycelia on gauze is collected, mycelia is stood after ultrasonic disruption, is centrifuged, collects supernatant
Liquid is up to mustard seed enzyme solution, after mustard seed enzyme solution carrier adsorption, myrosase system obtained by drying under the conditions of naturally dry or 22-30 DEG C
Agent.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution in 3-7, and the concentration of bacterium solution is 107-111/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 60-80%, glucose or sucrose 2-6%,
Yeast juice 10-14%, ferrous sulfate 0.02-0.06%, surplus are water, are uniformly mixed, pH is adjusted to 7.0-7.8；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:10-22, and oscillation and fermentation cultivation, fermentation temperature is 33-37 DEG C, fermentation time 4-6h, and hunting speed is
150-250rpm/min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 4-6h is carried out after being mixed evenly, it is cooling
After be filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is in 16-20
DEG C when relative density be 1.30-1.34；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
Preferably, in the step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, carry out powder
It is broken, cross 100-200 mesh, deionized water be added, the ratio of seaweed and deionized water is 1:4-6 part, be stirred it is uniform, then
Heating is dezymotized, and suspension is obtained, and the pH value for adjusting suspension is 7.0-7.4, and cellulase, neutral proteinase is added, and stirring is equal
It is even, ultrasonic wave enzymatic hydrolysis is carried out while agitating, and hydrolysis temperature is 40-60 DEG C, ultrasonic power 200-400W, the time of enzymatic hydrolysis
For 20-40min, enzymatic hydrolysis carries out enzyme deactivation after terminating, centrifugation takes supernatant to get seaweed extracted liquor.
Preferably, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution in 4-6, and the concentration of bacterium solution is 108-110/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 65-75%, glucose or sucrose 3-5%,
Yeast juice 11-13%, ferrous sulfate 0.03-0.05%, surplus are water, are uniformly mixed, pH is adjusted to 7.2-7.6；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:13-19, and oscillation and fermentation cultivation, fermentation temperature is 34-36 DEG C, fermentation time 4.5-5.5h, oscillation speed
Degree is 175-225rpm/min, obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, carry out decoction 4.5-5.5h after being mixed evenly,
It is filtered after cooling, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate exists
Relative density at 17-19 DEG C is 1.31-1.33；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
Preferably, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 5, and the concentration of bacterium solution is 109/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 70%, glucose or sucrose 4%, yeast juice
12%, ferrous sulfate 0.04%, surplus is water, is uniformly mixed, pH is adjusted to 7.4；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:16, and oscillation and fermentation cultivation, fermentation temperature is 35 DEG C, fermentation time 5h, hunting speed 200rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 5h are carried out after being mixed evenly, after cooling
It is filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 18 DEG C
Relative density be 1.32；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
Beneficial effects of the present invention:
(1), biological agent prepared by the present invention is added to microbial cells and traditional Chinese medicinal components, the use of microbial cells,
The drug effect for further enhancing Chinese medicine and bacteriostatic agent saves cost, and the addition of Chinese medicine reduces the usage amount of pesticide, drop
Low pesticide is endangered caused by human health, can be effectively prevented and treat plant leaf mold, while ensure that the quality of plant
And yield.
(2), the present invention adds seaweed extracted liquor in preparing nutrient solution, and seaweed extracted liquor is made jointly with microbial cells
With preventing the loss of microbial cells, realize the organic unity of seaweed and microbial cells.
(3), microbial cells and bacteriostatic agent being used in combination in the present invention, resulting biological agent is scientific, reasonable,
Nutrient solution is prepared with seaweed extracted liquor, meets the needs of the growth and development of plant, the collective effect of each component in biological agent
Abiotic persecution is reduced, the yield of plant is improved.
Specific embodiment
Below with reference to embodiment, further description of the specific embodiments of the present invention, and following embodiment is only used for more
Technical solution of the present invention is clearly demonstrated, and not intended to limit the protection scope of the present invention.
Embodiment 1
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 48 parts of microbial cells, antibacterial
20 parts of agent, 25 parts of traditional Chinese medicinal components, 60 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: mustard seed enzyme preparation 12
Part, 10 parts of colloid bacillus cereus, 15 parts of nitrogen-fixing bacteria, 11 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: 4 parts of chinaberry, 4 parts of alumen, plant glue 15 parts of powder.
Bacteriostatic agent includes the raw material of following parts by weight: 2 parts of Huang Shi purified powder, 3 parts of horseradish extract, poly- astatine pyrimethanil 10
Part, dish fruit strengthen 0.2 part of base of a fruit spirit.
Solvent is ethyl alcohol；Plant glues the raw material that powder includes following mass percent: water hemp skin powder 41%, elm bark powder are
59%.
Mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into be equipped with
Vibration culture 2 days in the flask of fluid nutrient medium, cultivation temperature are 25 DEG C, liquid pure culture are obtained, by liquid pure culture
Cross gauze, collect the mycelia on gauze, mycelia is stood after ultrasonic disruption, is centrifuged, collection supernatant up to mustard seed enzyme solution,
After mustard seed enzyme solution carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 22 DEG C.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 3, and the concentration of bacterium solution is 107/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 60%, glucose or sucrose 2%, yeast juice
10%, ferrous sulfate 0.02%, surplus is water, is uniformly mixed, pH is adjusted to 7.0；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:10, and oscillation and fermentation cultivation, fermentation temperature is 33 DEG C, fermentation time 4h, hunting speed 150rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 4h are carried out after being mixed evenly, after cooling
It is filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 16 DEG C
Relative density be 1.30；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
In step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, crushed, cross 100 mesh
The ratio of sieve, addition deionized water, seaweed and deionized water is 1:4 parts, is stirred uniformly, and then heating is dezymotized, and is hanged
Supernatant liquid, the pH value for adjusting suspension is 7.0, and cellulase, neutral proteinase is added, stirs evenly, carries out while agitating
Ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 40 DEG C, ultrasonic power 200W, and the time of enzymatic hydrolysis is 20min, and enzymatic hydrolysis goes out after terminating
Enzyme, centrifugation, takes supernatant to get seaweed extracted liquor.
Embodiment 2
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 54 parts of microbial cells, antibacterial
25 parts of agent, 30 parts of traditional Chinese medicinal components, 65 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: mustard seed enzyme preparation 13
Part, 12 parts of colloid bacillus cereus, 17 parts of nitrogen-fixing bacteria, 12 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: 5 parts of chinaberry, 6 parts of alumen, plant glue 16 parts of powder.
Bacteriostatic agent includes the raw material of following parts by weight: 3 parts of Huang Shi purified powder, 5 parts of horseradish extract, poly- astatine pyrimethanil 12
Part, dish fruit strengthen 0.3 part of base of a fruit spirit.
Solvent is ethyl alcohol；Plant glues the raw material that powder includes following mass percent: water hemp skin powder 42%, elm bark powder are
58%.
Mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into be equipped with
Vibration culture 2.5 days in the flask of fluid nutrient medium, cultivation temperature are 26 DEG C, liquid pure culture are obtained, by liquid pure culture
Object crosses gauze, collects the mycelia on gauze, mycelia is stood after ultrasonic disruption, is centrifuged, and collects supernatant up to myrosase
Liquid, after mustard seed enzyme solution carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 24 DEG C.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 4, and the concentration of bacterium solution is 108/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 65-75%, glucose or sucrose 3-5%,
Yeast juice 11%, ferrous sulfate 0.03%, surplus are water, are uniformly mixed, pH is adjusted to 7.2；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:13, and oscillation and fermentation cultivation, fermentation temperature is 34 DEG C, fermentation time 4.5h, hunting speed 175rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 4.5h is carried out after being mixed evenly, it is cooling
After be filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 17 DEG C
When relative density be 1.31；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
In step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, crushed, cross 125 mesh
The ratio of sieve, addition deionized water, seaweed and deionized water is 1:4.5 parts, is stirred uniformly, and then heating is dezymotized, and is obtained
Suspension, adjust suspension pH value be 7.1, be added cellulase, neutral proteinase, stir evenly, while agitating into
Row ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 45 DEG C, ultrasonic power 250W, and the time of enzymatic hydrolysis is 25min, and enzymatic hydrolysis carries out after terminating
Enzyme deactivation, centrifugation, takes supernatant to get seaweed extracted liquor.
Embodiment 3
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 60 parts of microbial cells, antibacterial
30 parts of agent, 35 parts of traditional Chinese medicinal components, 70 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: mustard seed enzyme preparation 14
Part, 14 parts of colloid bacillus cereus, 19 parts of nitrogen-fixing bacteria, 13 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: 6 parts of chinaberry, 8 parts of alumen, plant glue 17 parts of powder.
Bacteriostatic agent includes the raw material of following parts by weight: 4 parts of Huang Shi purified powder, 7 parts of horseradish extract, poly- astatine pyrimethanil 14
Part, dish fruit strengthen 0.4 part of base of a fruit spirit.
Solvent is ethyl alcohol；The plant glues the raw material that powder includes following mass percent: water hemp skin powder 43%, elm bark powder
It is 57%.
Mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into be equipped with
Vibration culture 3 days in the flask of fluid nutrient medium, cultivation temperature are 27 DEG C, liquid pure culture are obtained, by liquid pure culture
Cross gauze, collect the mycelia on gauze, mycelia is stood after ultrasonic disruption, is centrifuged, collection supernatant up to mustard seed enzyme solution,
After mustard seed enzyme solution carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 26 DEG C.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 5, and the concentration of bacterium solution is 109/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 70%, glucose or sucrose 4%, yeast juice
12%, ferrous sulfate 0.04%, surplus is water, is uniformly mixed, pH is adjusted to 7.4；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:16, and oscillation and fermentation cultivation, fermentation temperature is 35 DEG C, fermentation time 5h, hunting speed 200rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 5h are carried out after being mixed evenly, after cooling
It is filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 18 DEG C
Relative density be 1.32；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
In step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, crushed, cross 150 mesh
The ratio of sieve, addition deionized water, seaweed and deionized water is 1:5 parts, is stirred uniformly, and then heating is dezymotized, and is hanged
Supernatant liquid, the pH value for adjusting suspension is 7.2, and cellulase, neutral proteinase is added, stirs evenly, carries out while agitating
Ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 50 DEG C, ultrasonic power 300W, and the time of enzymatic hydrolysis is 30min, and enzymatic hydrolysis goes out after terminating
Enzyme, centrifugation, takes supernatant to get seaweed extracted liquor.
Embodiment 4
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 66 parts of microbial cells, antibacterial
35 parts of agent, 40 parts of traditional Chinese medicinal components, 75 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: mustard seed enzyme preparation 15
Part, 16 parts of colloid bacillus cereus, 21 parts of nitrogen-fixing bacteria, 14 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: 7 parts of chinaberry, 10 parts of alumen, plant glue 18 parts of powder.
Bacteriostatic agent includes the raw material of following parts by weight: 5 parts of Huang Shi purified powder, 9 parts of horseradish extract, poly- astatine pyrimethanil 16
Part, dish fruit strengthen 0.5 part of base of a fruit spirit.
Solvent is ethyl alcohol；The plant glues the raw material that powder includes following mass percent: water hemp skin powder 44%, elm bark powder
It is 56%.
Mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into be equipped with
Vibration culture 3.5 days in the flask of fluid nutrient medium, cultivation temperature are 28 DEG C, liquid pure culture are obtained, by liquid pure culture
Object crosses gauze, collects the mycelia on gauze, mycelia is stood after ultrasonic disruption, is centrifuged, and collects supernatant up to myrosase
Liquid, after mustard seed enzyme solution carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 28 DEG C.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 6, and the concentration of bacterium solution is 110/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 75%, glucose or sucrose 5%, yeast juice
13%, ferrous sulfate 0.05%, surplus is water, is uniformly mixed, pH is adjusted to 7.6；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:19, and oscillation and fermentation cultivation, fermentation temperature is 36 DEG C, fermentation time 5.5h, hunting speed 225rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 5.5h is carried out after being mixed evenly, it is cooling
After be filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 19 DEG C
When relative density be 1.33；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
In step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, crushed, cross 175 mesh
The ratio of sieve, addition deionized water, seaweed and deionized water is 1:5.5 parts, is stirred uniformly, and then heating is dezymotized, and is obtained
Suspension, adjust suspension pH value be 7.3, be added cellulase, neutral proteinase, stir evenly, while agitating into
Row ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 55 DEG C, ultrasonic power 350W, and the time of enzymatic hydrolysis is 35min, and enzymatic hydrolysis carries out after terminating
Enzyme deactivation, centrifugation, takes supernatant to get seaweed extracted liquor.
Embodiment 5
A kind of prevention and treatment biological agent of plant leaf mold, the raw material including following parts by weight: 72 parts of microbial cells, antibacterial
40 parts of agent, 45 parts of traditional Chinese medicinal components, 80 parts of solvent；Wherein microbial cells include the raw material of following parts by weight: mustard seed enzyme preparation 16
Part, 18 parts of colloid bacillus cereus, 23 parts of nitrogen-fixing bacteria, 15 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include following parts by weight
Raw material: 8 parts of chinaberry, 12 parts of alumen, plant glue 19 parts of powder.
Bacteriostatic agent includes the raw material of following parts by weight: 6 parts of Huang Shi purified powder, 11 parts of horseradish extract, poly- astatine pyrimethanil 18
Part, dish fruit strengthen 0.6 part of base of a fruit spirit.
Solvent is ethyl alcohol；Plant glues the raw material that powder includes following mass percent: water hemp skin powder 45%, elm bark powder are
55%.
Mustard seed enzyme preparation the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into be equipped with
Vibration culture 4 days in the flask of fluid nutrient medium, cultivation temperature are 29 DEG C, liquid pure culture are obtained, by liquid pure culture
Cross gauze, collect the mycelia on gauze, mycelia is stood after ultrasonic disruption, is centrifuged, collection supernatant up to mustard seed enzyme solution,
After mustard seed enzyme solution carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 30 DEG C.
The method for preparing a kind of prevention and treatment biological agent of plant leaf mold, comprising the following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium
Liquid compounds the pH range of bacterium solution 7, and the concentration of bacterium solution is 111/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 80%, glucose or sucrose 6%, yeast juice
14%, ferrous sulfate 0.06%, surplus is water, is uniformly mixed, pH is adjusted to 7.8；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and battalion
The ratio of nutrient solution is 1:22, and oscillation and fermentation cultivation, fermentation temperature is 37 DEG C, fermentation time 6h, hunting speed 250rpm/
Min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 6h are carried out after being mixed evenly, after cooling
It is filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 20 DEG C
Relative density be 1.34；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step
Suddenly the zymocyte liquid that (4) obtain adds the traditional Chinese medicinal components concentration that step (5) obtains after zymocyte liquid is sufficiently mixed completely
Liquid is stirred uniformly to get the prevention and treatment biological agent for arriving plant leaf mold.
In step (3) seaweed extracted liquor the preparation method is as follows: weigh the seaweed of parts by weight, crushed, cross 200 mesh
The ratio of sieve, addition deionized water, seaweed and deionized water is 1:6 parts, is stirred uniformly, and then heating is dezymotized, and is hanged
Supernatant liquid, the pH value for adjusting suspension is 7.4, and cellulase, neutral proteinase is added, stirs evenly, carries out while agitating
Ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 60 DEG C, ultrasonic power 400W, and the time of enzymatic hydrolysis is 40min, and enzymatic hydrolysis goes out after terminating
Enzyme, centrifugation, takes supernatant to get seaweed extracted liquor.
Comparative example group
Comparative example 1
The present invention relates to a kind of prevention and treatment biological agents of plant leaf mold, compared with Example 1, remove micro- life in component
Object thallus, remaining component and step are same as Example 1.
Comparative example 2
The present invention relates to a kind of prevention and treatment biological agents of plant leaf mold, compared with Example 1, remove the Chinese medicine in component
Component, remaining component and step are same as Example 1.
Comparative example 3
The present invention relates to a kind of prevention and treatment biological agents of plant leaf mold, compared with Example 1, remove in step (3)
The preparation method of seaweed extracted liquor and seaweed extracted liquor, remaining component and step are same as Example 1.
Below table is the performance test of embodiment 1-5 and comparative example 1-3:
| Project | 7 days preventive effects (%) after last medication | 14 days preventive effects (%) after last medication |
| Embodiment 1 | 88.10 | 92.31 |
| Embodiment 2 | 88.26 | 93.20 |
| Embodiment 3 | 87.91 | 91.87 |
| Embodiment 4 | 90.02 | 91.96 |
| Embodiment 5 | 91.23 | 94.53 |
| Comparative example 1 | 50.56 | 56.43 |
| Comparative example 2 | 61.80 | 68.62 |
| Comparative example 3 | 69.45 | 71.34 |
Above table result is that the prevention and treatment to leaf muld of tomato is tested, and is that 55-60kg progress is uniform by dosage per acre
It is spraying, it is administered 3 times, is divided between application every time 7 days altogether, the investigation result after the 3rd application.
From in above table as can be seen that 7 days preventive effects are all larger than 88% after last medication in embodiment 1-5, comparative example 1-3
7 days preventive effects are 50-70% after middle last medication, and 14 days preventive effects are all larger than 90% after last medication in embodiment 1-5, comparative example 1-
14 days preventive effects are 56-72% after last medication in 3, and the drug effect of embodiment 1-5 is better than the drug effect of comparative example 1-3 in summary,
Drug effect wherein in embodiment 5 is best.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although referring to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features.It is all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of prevention and treatment biological agent of plant leaf mold, it is characterised in that: the raw material including following parts by weight: microbial cells
48-72 parts, 20-40 parts of bacteriostatic agent, 25-45 parts of traditional Chinese medicinal components, 60-80 parts of solvent；Wherein microbial cells include following weight
The raw material of part: 12-16 parts of mustard seed enzyme preparation, 10-18 parts of colloid bacillus cereus, 15-23 parts of nitrogen-fixing bacteria, bacillus subtilis 11-
15 parts；Wherein traditional Chinese medicinal components include the raw material of following parts by weight: 4-8 parts of chinaberry, 4-12 parts of alumen, plant are powder 15-19 parts viscous.
2. a kind of prevention and treatment biological agent of plant leaf mold according to claim 1, it is characterised in that: including following weight
The raw material of part: 54-66 parts of microbial cells, 25-35 parts of bacteriostatic agent, 30-40 parts of traditional Chinese medicinal components, 65-75 parts of solvent；Wherein micro- life
Object thallus includes the raw material of following parts by weight: 13-15 parts of mustard seed enzyme preparation, 12-16 parts of colloid bacillus cereus, and nitrogen-fixing bacteria 17-21
Part, 12-14 parts of bacillus subtilis；Wherein traditional Chinese medicinal components include the raw material of following parts by weight: 5-7 parts of chinaberry, alumen 6-10
Part, plant are powder 16-18 parts viscous.
3. a kind of prevention and treatment biological agent of plant leaf mold according to claim 1, it is characterised in that: including following weight
The raw material of part: 60 parts of microbial cells, 30 parts of bacteriostatic agent, 35 parts of traditional Chinese medicinal components, 70 parts of solvent；Wherein microbial cells include with
The raw material of lower parts by weight: 14 parts of mustard seed enzyme preparation, 14 parts of colloid bacillus cereus, 19 parts of nitrogen-fixing bacteria, 13 parts of bacillus subtilis；Its
Middle traditional Chinese medicinal components include the raw material of following parts by weight: 6 parts of chinaberry, 8 parts of alumen, plant glue 17 parts of powder.
4. a kind of prevention and treatment biological agent of plant leaf mold according to claim 1, it is characterised in that: the bacteriostatic agent packet
Include the raw material of following parts by weight: 2-6 parts of Huang Shi purified powder, 3-11 parts of horseradish extract, 10-18 parts of poly- astatine pyrimethanil, dish fruit are strong
0.2-0.6 parts of base of a fruit spirit.
5. a kind of prevention and treatment biological agent of plant leaf mold according to claim 1, it is characterised in that: the solvent is second
Alcohol；The plant glues the raw material that powder includes following mass percent: water hemp skin powder 41-45%, and elm bark powder is 55-59%.
6. a kind of prevention and treatment biological agent of plant leaf mold according to claim 1, it is characterised in that: the myrosase system
Agent the preparation method is as follows: the bacterial strain of the dark green wooden enzyme is carried out pure culture, pure culture is to be put into the flask equipped with fluid nutrient medium
Middle vibration is cultivated 2-4 days, and cultivation temperature is 25-29 DEG C, obtains liquid pure culture, liquid pure culture is crossed gauze, is collected
Mycelia is stood after ultrasonic disruption, is centrifuged by the mycelia on gauze, collects supernatant up to mustard seed enzyme solution, by mustard seed enzyme solution
After carrier adsorption, mustard seed enzyme preparation obtained by drying under the conditions of naturally dry or 22-30 DEG C.
7. the method for preparing the prevention and treatment biological agent of any plant leaf mold described in -6 according to claim 1, feature
It is: the following steps are included:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium liquid, multiple
PH range with bacterium solution is 107-111/ml in 3-7, the concentration of bacterium solution；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 60-80%, glucose or sucrose 2-6%, yeast
Juice 10-14%, ferrous sulfate 0.02-0.06%, surplus are water, are uniformly mixed, pH is adjusted to 7.0-7.8；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and nutrient solution
Ratio be 1:10-22, oscillation and fermentation cultivation, fermentation temperature be 33-37 DEG C, fermentation time 4-6h, hunting speed 150-
250rpm/min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 4-6h is carried out after being mixed evenly, cooling is laggard
Row filtering, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is at 16-20 DEG C
Relative density be 1.30-1.34；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step (4)
Obtained zymocyte liquid adds the traditional Chinese medicinal components concentrate that step (5) obtains after zymocyte liquid is sufficiently mixed completely, carries out
It stirs evenly to get the prevention and treatment biological agent of plant leaf mold is arrived.
8. a kind of method of the prevention and treatment biological agent according to claim 7 for preparing plant leaf mold, it is characterised in that: institute
State seaweed extracted liquor in step (3) the preparation method is as follows: weighs the seaweed of parts by weight, is crushed, crosses 100-200 mesh,
The ratio of addition deionized water, seaweed and deionized water is 1:4-6 parts, is stirred uniformly, and then heating is dezymotized, and is suspended
Liquid, adjust suspension pH value be 7.0-7.4, be added cellulase, neutral proteinase, stir evenly, while agitating into
Row ultrasonic wave enzymatic hydrolysis, hydrolysis temperature are 40-60 DEG C, ultrasonic power 200-400W, and the time of enzymatic hydrolysis is 20-40min, enzymatic hydrolysis knot
Enzyme deactivation is carried out after beam, is centrifuged, takes supernatant to get seaweed extracted liquor.
9. a kind of method of the prevention and treatment biological agent according to claim 7 for preparing plant leaf mold, it is characterised in that: packet
Include following steps:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium liquid, multiple
PH range with bacterium solution is 108-110/ml in 4-6, the concentration of bacterium solution；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 65-75%, glucose or sucrose 3-5%, yeast
Juice 11-13%, ferrous sulfate 0.03-0.05%, surplus are water, are uniformly mixed, pH is adjusted to 7.2-7.6；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and nutrient solution
Ratio be 1:13-19, oscillation and fermentation cultivation, fermentation temperature is 34-36 DEG C, fermentation time 4.5-5.5h, and hunting speed is
175-225rpm/min obtains zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 4.5-5.5h is carried out after being mixed evenly, it is cooling
After be filtered, then traditional Chinese medicinal components decoction liquor be concentrated to give and buys traditional Chinese medicinal components concentrate, traditional Chinese medicinal components concentrate is in 17-19
DEG C when relative density be 1.31-1.33；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step (4)
Obtained zymocyte liquid adds the traditional Chinese medicinal components concentrate that step (5) obtains after zymocyte liquid is sufficiently mixed completely, carries out
It stirs evenly to get the prevention and treatment biological agent of plant leaf mold is arrived.
10. a kind of method of the prevention and treatment biological agent according to claim 7 for preparing plant leaf mold, it is characterised in that:
The following steps are included:
(1), the raw material of above-mentioned parts by weight is weighed；
(2), by mustard seed enzyme preparation, colloid bacillus cereus, nitrogen-fixing bacteria, bacillus subtilis, which is uniformly mixed, becomes Compound bacterium liquid, multiple
For pH range with bacterium solution 5, the concentration of bacterium solution is 109/ml；
(3), prepare nutrient solution: the ingredient of nutrient solution includes: seaweed extracted liquor 70%, glucose or sucrose 4%, yeast juice
12%, ferrous sulfate 0.04%, surplus is water, is uniformly mixed, pH is adjusted to 7.4；
(4), the bacterium solution for obtaining step (2) is added in the triangular flask of the nutrient solution equipped with step (3) preparation, bacterium solution and nutrient solution
Ratio be 1:16, oscillation and fermentation cultivation, fermentation temperature be 35 DEG C, fermentation time 5h, hunting speed 200rpm/min obtain
To zymocyte liquid；
(5), traditional Chinese medicinal components and deionized water are added in reaction pot, decoction 5h is carried out after being mixed evenly, is carried out after cooling
Filtering, then traditional Chinese medicinal components decoction liquor be concentrated to give and beats traditional Chinese medicinal components concentrate, phase of the traditional Chinese medicinal components concentrate at 18 DEG C
It is 1.32 to density；
(6), solvent is added in reaction vessel, bacteriostatic agent is then added and is stirred uniformly, is stirring evenly and then adding into step (4)
Obtained zymocyte liquid adds the traditional Chinese medicinal components concentrate that step (5) obtains after zymocyte liquid is sufficiently mixed completely, carries out
It stirs evenly to get the prevention and treatment biological agent of plant leaf mold is arrived.
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Application publication date: 20190212 |